Q9H4D0 (CSTN2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Calsyntenin-2

Alternative name(s):
Alcadein-gamma
Short name=Alc-gamma

Gene names

Name:	CLSTN2
Synonyms:	CS2

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	955 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May modulate calcium-mediated postsynaptic signals By similarity.
Subcellular location	Cell membrane; Single-pass type I membrane protein Potential. Endoplasmic reticulum membrane. Golgi apparatus membrane. Note: Most prominent in the postsynaptic specializations of asymmetric (type I) synapses with both axodendritic and axospinous localization By similarity.
Tissue specificity	Restricted to the brain. Ref.1
Domain	Binds synaptic Ca2+ with its cytoplasmic domain By similarity.
Post-translational modification	Proteolytically processed under normal cellular conditions. A primary zeta-cleavage generates a large extracellular (soluble) N-terminal domain (sAlc) and a short C-terminal transmembrane fragment (CTF1). A secondary cleavage catalyzed by gamma-secretase within the transmembrane domain releases the beta-Alc-gamma chain in the extracellular milieu and produces an intracellular fragment (AlcICD). This processing is strongly suppressed in the tripartite complex formed with APBA2 and APP, which seems to prevent the association with PSEN1 By similarity.
Sequence similarities	Contains 2 cadherin domains.

Ontologies

Keywords
Biological process	Cell adhesion
Cellular component	Cell membrane Endoplasmic reticulum Golgi apparatus Membrane
Coding sequence diversity	Polymorphism
Domain	Repeat Signal Transmembrane Transmembrane helix
Ligand	Calcium
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	homophilic cell adhesion Inferred from electronic annotation. Source: InterPro
Cellular_component	Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell postsynaptic membrane Inferred from electronic annotation. Source: Compara
Molecular_function	calcium ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 20	20	Potential
	Chain	21 – 955	935	Calsyntenin-2		PRO_0000004023
Regions
	Topological domain	21 – 831	811	Extracellular Potential
	Transmembrane	832 – 852	21	Helical; Potential
	Topological domain	853 – 955	103	Cytoplasmic Potential
	Domain	44 – 160	117	Cadherin 1
	Domain	161 – 280	120	Cadherin 2
	Compositional bias	889 – 929	41	Glu-rich (highly acidic)
Amino acid modifications
	Glycosylation	56	1	N-linked (GlcNAc...) Potential
	Glycosylation	98	1	N-linked (GlcNAc...) Potential
	Glycosylation	342	1	N-linked (GlcNAc...) Potential
	Glycosylation	374	1	N-linked (GlcNAc...) Potential
	Glycosylation	716	1	N-linked (GlcNAc...) Potential
	Glycosylation	729	1	N-linked (GlcNAc...) Potential
Natural variations
	Natural variant	193	1	S → I in a colorectal cancer sample; somatic mutation. Ref.8		VAR_036112
	Natural variant	331	1	I → T. Corresponds to variant rs17348572 [ dbSNP | Ensembl ].		VAR_055615
	Natural variant	366	1	V → I. Ref.1 Ref.2 Ref.5 Corresponds to variant rs7632885 [ dbSNP | Ensembl ].		VAR_039557
	Natural variant	765	1	R → Q in a colorectal cancer sample; somatic mutation. Ref.8		VAR_036113
Experimental info
	Sequence conflict	847	1	V → D in AAI03507. Ref.6
	Sequence conflict	946	1	L → P in AAI03507. Ref.6

Sequences

	Sequence		Length	Mass (Da)
	Q9H4D0 [UniParc]. Last modified May 18, 2010. Version 2. Checksum: 6059F3277F152E75	FASTA	955	107,006
	10 20 30 40 50 60 MLPGRLCWVP LLLALGVGSG SGGGGDSRQR RLLAAKVNKH KPWIETSYHG VITENNDTVI 70 80 90 100 110 120 LDPPLVALDK DAPVPFAGEI CAFKIHGQEL PFEAVVLNKT SGEGRLRAKS PIDCELQKEY 130 140 150 160 170 180 TFIIQAYDCG AGPHETAWKK SHKAVVHIQV KDVNEFAPTF KEPAYKAVVT EGKIYDSILQ 190 200 210 220 230 240 VEAIDEDCSP QYSQICNYEI VTTDVPFAID RNGNIRNTEK LSYDKQHQYE ILVTAYDCGQ 250 260 270 280 290 300 KPAAQDTLVQ VDVKPVCKPG WQDWTKRIEY QPGSGSMPLF PSIHLETCDG AVSSLQIVTE 310 320 330 340 350 360 LQTNYIGKGC DRETYSEKSL QKLCGASSGI IDLLPSPSAA TNWTAGLLVD SSEMIFKFDG 370 380 390 400 410 420 RQGAKVPDGI VPKNLTDQFT ITMWMKHGPS PGVRAEKETI LCNSDKTEMN RHHYALYVHN 430 440 450 460 470 480 CRLVFLLRKD FDQADTFRPA EFHWKLDQIC DKEWHYYVIN VEFPVVTLYM DGATYEPYLV 490 500 510 520 530 540 TNDWPIHPSH IAMQLTVGAC WQGGEVTKPQ FAQFFHGSLA SLTIRPGKME SQKVISCLQA 550 560 570 580 590 600 CKEGLDINSL ESLGQGIKYH FNPSQSILVM EGDDIGNINR ALQKVSYINS RQFPTAGVRR 610 620 630 640 650 660 LKVSSKVQCF GEDVCISIPE VDAYVMVLQA IEPRITLRGT DHFWRPAAQF ESARGVTLFP 670 680 690 700 710 720 DIKIVSTFAK TEAPGDVKTT DPKSEVLEEM LHNLDFCDIL VIGGDLDPRQ ECLELNHSEL 730 740 750 760 770 780 HQRHLDATNS TAGYSIYGVG SMSRYEQVLH HIRYRNWRPA SLEARRFRIK CSELNGRYTS 790 800 810 820 830 840 NEFNLEVSIL HEDQVSDKEH VNHLIVQPPF LQSVHHPESR SSIQHSSVVP SIATVVIIIS 850 860 870 880 890 900 VCMLVFVVAM GVYRVRIAHQ HFIQETEAAK ESEMDWDDSA LTITVNPMEK HEGPGHGEDE 910 920 930 940 950 TEGEEEEEAE EEMSSSSGSD DSEEEEEEEG MGRGRHGQNG ARQAQLEWDD STLPY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The calsyntenins - a family of postsynaptic membrane proteins with distinct neuronal expression patterns." Hintsch G., Zurlinden A., Meskenaite V., Steuble M., Fink-Widmer K., Kinter J., Sonderegger P. Mol. Cell. Neurosci. 21:393-409(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, VARIANT ILE-366. Tissue: Fetal brain.
[2]	"Novel cadherin-related membrane proteins, Alcadeins, enhance the X11-like protein-mediated stabilization of amyloid beta-protein precursor metabolism." Araki Y., Tomita S., Yamaguchi H., Miyagi N., Sumioka A., Kirino Y., Suzuki T. J. Biol. Chem. 278:49448-49458(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT ILE-366.
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Uterus.
[4]	"The DNA sequence, annotation and analysis of human chromosome 3." Muzny D.M., Scherer S.E., Kaul R., Wang J., Yu J., Sudbrak R., Buhay C.J., Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J. , Jackson A., Khan Z.M., Kovar-Smith C., Lewis L.R., Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R., Morgan M.B., Nazareth L.V., Scott G., Sodergren E., Song X.-Z., Steffen D., Wei S., Wheeler D.A., Wright M.W., Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G., Chen Z., Clendenning J., Clerc-Blankenburg K.P., Chen R., Chen Z., Davis C., Delgado O., Dinh H.H., Dong W., Draper H., Ernst S., Fu G., Gonzalez-Garay M.L., Garcia D.K., Gillett W., Gu J., Hao B., Haugen E., Havlak P., He X., Hennig S., Hu S., Huang W., Jackson L.R., Jacob L.S., Kelly S.H., Kube M., Levy R., Li Z., Liu B., Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O., Palmeiri A., Pasternak S., Perez L.M., Phelps K.A., Plopper F.J., Qiang B., Raymond C., Rodriguez R., Saenphimmachak C., Santibanez J., Shen H., Shen Y., Subramanian S., Tabor P.E., Verduzco D., Waldron L., Wang J., Wang J., Wang Q., Williams G.A., Wong G.K.-S., Yao Z., Zhang J., Zhang X., Zhao G., Zhou J., Zhou Y., Nelson D., Lehrach H., Reinhardt R., Naylor S.L., Yang H., Olson M., Weinstock G., Gibbs R.A. Nature 440:1194-1198(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], VARIANT ILE-366.
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain and Lung.
[7]	"Coordinated metabolism of Alcadein and amyloid beta-protein precursor regulates FE65-dependent gene transactivation." Araki Y., Miyagi N., Kato N., Yoshida T., Wada S., Nishimura M., Komano H., Yamamoto T., De Strooper B., Yamamoto K., Suzuki T. J. Biol. Chem. 279:24343-24354(2004) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEOLYTIC CLEAVAGE.
[8]	"The consensus coding sequences of human breast and colorectal cancers." Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D., Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P., Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V. , Gazdar A.F., Hartigan J., Wu L., Liu C., Parmigiani G., Park B.H., Bachman K.E., Papadopoulos N., Vogelstein B., Kinzler K.W., Velculescu V.E. Science 314:268-274(2006) [PubMed] [Europe PMC] [Abstract] Cited for: VARIANTS [LARGE SCALE ANALYSIS] ILE-193 AND GLN-765.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ278018 mRNA. Translation: CAC14924.1. AY753303 mRNA. Translation: AAV30553.1. AK315308 mRNA. Translation: BAG37712.1. AC010181 Genomic DNA. No translation available. AC048346 Genomic DNA. No translation available. AC092988 Genomic DNA. No translation available. AC108744 Genomic DNA. No translation available. AC117390 Genomic DNA. No translation available. AC117396 Genomic DNA. No translation available. AC117449 Genomic DNA. No translation available. CH471052 Genomic DNA. Translation: EAW79018.1. BC004871 mRNA. Translation: AAH04871.2. BC007943 mRNA. Translation: AAH07943.2. BC103496 mRNA. Translation: AAI03497.1. BC103506 mRNA. Translation: AAI03507.1. BC104485 mRNA. Translation: AAI04486.1.
IPI	IPI00005491.
RefSeq	NP_071414.2. NM_022131.2.
UniGene	Hs.158529.
3D structure databases
ProteinModelPortal	Q9H4D0.
SMR	Q9H4D0. Positions 148-254.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000402460.
PTM databases
PhosphoSite	Q9H4D0.
Polymorphism databases
DMDM	296434469.
Proteomic databases
PaxDb	Q9H4D0.
PRIDE	Q9H4D0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000458420; ENSP00000402460; ENSG00000158258.
GeneID	64084.
KEGG	hsa:64084.
UCSC	uc003etm.2. human.
Organism-specific databases
CTD	64084.
GeneCards	GC03P139654.
H-InvDB	HIX0003719.
HGNC	HGNC:17448. CLSTN2.
HPA	HPA031342.
MIM	611323. gene.
neXtProt	NX_Q9H4D0.
PharmGKB	PA38239.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG246161.
HOVERGEN	HBG051146.
InParanoid	Q9H4D0.
OMA	AKPRFAQ.
OrthoDB	EOG4XSKP5.
PhylomeDB	Q9H4D0.
Gene expression databases
ArrayExpress	Q9H4D0.
Bgee	Q9H4D0.
CleanEx	HS_CLSTN2.
Genevestigator	Q9H4D0.
GermOnline	ENSG00000158258. Homo sapiens.
Family and domain databases
Gene3D	2.60.120.200. 1 hit. 2.60.40.60. 2 hits.
InterPro	IPR002126. Cadherin. IPR015919. Cadherin-like. IPR026914. Calsyntenin. IPR008985. ConA-like_lec_gl_sf. IPR013320. ConA-like_subgrp. [Graphical view]
PANTHER	PTHR14139. PTHR14139. 1 hit.
Pfam	PF00028. Cadherin. 1 hit. [Graphical view]
PRINTS	PR00205. CADHERIN.
SMART	SM00112. CA. 2 hits. [Graphical view]
SUPFAM	SSF49313. Cadherin. 2 hits. SSF49899. ConA_like_lec_gl. 1 hit.
PROSITE	PS00232. CADHERIN_1. False negative. PS50268. CADHERIN_2. 2 hits. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	64084.
NextBio	65880.
SOURCE	Search...

Entry information

Entry name

CSTN2_HUMAN

Accession

Primary (citable) accession number: Q9H4D0
Secondary accession number(s): B2RCW5 , D3DNF4, Q3SX54, Q3ZB76, Q5UE56, Q96HZ2, Q9BSS0

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 19, 2002
Last sequence update:	May 18, 2010
Last modified:	May 1, 2013
This is version 105 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.