Reviewed, UniProtKB/Swiss-Prot Q9H4B6 (SAV1_HUMAN)

Names and origin

Protein names

Recommended name:
    Protein salvador homolog 1
Alternative name(s):
    45 kDa WW domain protein
      Short name=hWW45

Gene names

Name:	SAV1
Synonyms:	WW45

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	383 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Potential scaffold protein. Ref.6
Subunit structure	Homodimer. Stabilized through interaction with STK3 or STK4. Ref.6
Tissue specificity	Ubiquitously expressed in adult tissues with highest expression in the pancreas, aorta and interventricular septum and lowest expression in skeletal muscle. Expression was higher in fetal than in the adult heart.
Post-translational modification	Phosphorylated by STK3 and STK4. Phosphorylation is not required for SAV1 stability and may increase the number of protein binding sites on the scaffold molecule. Ref.6 Ref.7
Sequence similarities	Contains 1 SARAH domain. Contains 2 WW domains.

Ontologies

Keywords
Disease	Disease mutation
Domain	Coiled coil Repeat
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	signal transduction Inferred from electronic annotation. Source: InterPro
Molecular function	identical protein binding Ref.6 Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
itself		1	EBI-1017775,EBI-1017775
STK3	Q13188	2	EBI-1017775,EBI-992580
STK4	Q13043	2	EBI-1017775,EBI-367376

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 383	383	Protein salvador homolog 1		PRO_0000076060
Regions
	Domain	199 – 232	34	WW 1
	Domain	234 – 267	34	WW 2
	Domain	321 – 368	48	SARAH
	Coiled coil	344 – 373	30	Potential
Amino acid modifications
	Modified residue	26	1	Phosphothreonine Ref.7
Natural variations
	Natural variant	185	1	A → D in a colon cancer cell line. Ref.8		VAR_015880
Experimental info
	Sequence conflict	5	1	K → Q in CAC13972. Ref.1
	Sequence conflict	18	1	Q → R in CAG33578. Ref.3
	Sequence conflict	292	1	L → F in BAB13835. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q9H4B6-1 [UniParc]. Last modified July 11, 2003. Version 2. Checksum: 4012C40A8601417A	FASTA	383	44,634
	10 20 30 40 50 60 MLSRKKTKNE VSKPAEVQGK YVKKETSPLL RNLMPSFIRH GPTIPRRTDI CLPDSSPNAF 70 80 90 100 110 120 STSGDVVSRN QSFLRTPIQR TPHEIMRRES NRLSAPSYLA RSLADVPREY GSSQSFVTEV 130 140 150 160 170 180 SFAVENGDSG SRYYYSDNFF DGQRKRPLGD RAHEDYRYYE YNHDLFQRMP QNQGRHASGI 190 200 210 220 230 240 GRVAATSLGN LTNHGSEDLP LPPGWSVDWT MRGRKYYIDH NTNTTHWSHP LEREGLPPGW 250 260 270 280 290 300 ERVESSEFGT YYVDHTNKKA QYRHPCAPSV PRYDQPPPVT YQPQQTERNQ SLLVPANPYH 310 320 330 340 350 360 TAEIPDWLQV YARAPVKYDH ILKWELFQLA DLDTYQGMLK LLFMKELEQI VKMYEAYRQA 370 380 LLTELENRKQ RQQWYAQQHG KNF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning, expression and mapping of hWW45, a novel WW-domain containing gene." Valverde P. Biochem. Biophys. Res. Commun. 276:990-998(2000) [PubMed: 11027580] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Fetus and Neuron.
[3]	"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Kidney.
[5]	"The Ste20-like kinase Mst2 activates the human large tumor suppressor kinase Lats1." Chan E.H.Y., Nousiainen M., Chalamalasetty R.B., Schaefer A., Nigg E.A., Sillje H.H.W. Oncogene 24:2076-2086(2005) [PubMed: 15688006] [Abstract] Cited for: INTERACTION WITH STK3.
[6]	"Association of mammalian sterile twenty kinases, Mst1 and Mst2, with hSalvador via C-terminal coiled-coil domains, leads to its stabilization and phosphorylation." Callus B.A., Verhagen A.M., Vaux D.L. FEBS J. 273:4264-4276(2006) [PubMed: 16930133] [Abstract] Cited for: FUNCTION, HOMODIMERIZATION, INTERACTION WITH STK3 AND STK4, PHOSPHORYLATION.
[7]	"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle." Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M. Mol. Cell 31:438-448(2008) [PubMed: 18691976] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-26, MASS SPECTROMETRY.
[8]	"Salvador promotes both cell cycle exit and apoptosis in Drosophila and is mutated in human cancer cell lines." Tapon N., Harvey K.F., Bell D.W., Wahrer D.C.R., Schiripo T.A., Haber D.A., Hariharan I.K. Cell 110:467-478(2002) [PubMed: 12202036] [Abstract] Cited for: VARIANT ASP-185.
+	Additional computationally mapped references.

Web resources

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ292969 mRNA. Translation: CAC13972.1. AK021500 mRNA. Translation: BAB13835.1. AK023071 mRNA. Translation: BAB14390.1. CR457297 mRNA. Translation: CAG33578.1. BC020537 mRNA. Translation: AAH20537.1.
IPI	IPI00301738.
PIR	JC7507.
RefSeq	NP_068590.1.
UniGene	Hs.642842
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9H4B6. 3 interactions.
PTM databases
PhosphoSite	Q9H4B6.
Proteomic databases
PRIDE	Q9H4B6.
Genome annotation databases
Ensembl	ENSG00000151748. Homo sapiens. [Contig view]
GeneID	60485.
KEGG	hsa:60485.
Organism-specific databases
GeneCards	GC14M050170.
H-InvDB	HIX0011644.
HGNC	HGNC:17795. SAV1.
MIM	607203. gene.
PharmGKB	PA134875018.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	Q9H4B6.
HOVERGEN	Q9H4B6.
OMA	Q9H4B6. DWTIRGR.
Gene expression databases
ArrayExpress	Q9H4B6.
Bgee	Q9H4B6.
CleanEx	HS_SAV1.
GermOnline	ENSG00000151748. Homo sapiens.
Family and domain databases
InterPro	IPR011524. SARAH. IPR001202. WW_Rsp5_WWP. [Graphical view]
Pfam	PF00397. WW. 2 hits. [Graphical view]
SMART	SM00456. WW. 2 hits. [Graphical view]
PROSITE	PS50951. SARAH. 1 hit. PS01159. WW_DOMAIN_1. False negative. PS50020. WW_DOMAIN_2. 2 hits. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	65363.
SOURCE	Search...

Entry information

Entry name

SAV1_HUMAN

Accession

Primary (citable) accession number: Q9H4B6
Secondary accession number(s): Q6IA58, Q9H949, Q9HAK9

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 11, 2003
Last sequence update:	July 11, 2003
Last modified:	June 16, 2009
This is version 53 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)