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Reviewed, UniProtKB/Swiss-Prot Q9H4B4 (PLK3_HUMAN)

Last modified February 9, 2010. Version 92. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Web resources · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Serine/threonine-protein kinase PLK3
    EC=2.7.11.21
Alternative name(s):
    Polo-like kinase 3
      Short name=PLK-3
    Cytokine-inducible serine/threonine-protein kinase
    FGF-inducible kinase
    Proliferation-related kinase
Gene names
Name: PLK3
Synonyms: CNK, FNK, PRK
OrganismHomo sapiens (Human) [Complete proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length646 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

Serine/threonine protein kinase involved in regulating M phase functions during the cell cycle. May also be part of the signaling network controlling cellular adhesion. In vitro, is able to phosphorylate CDC25C and casein.

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

Subunit structure

Binds to the calcium/integrin-binding protein (CIB). This interaction probably occurs via the POLO-box domain.

Subcellular location

Membrane; Peripheral membrane protein.

Tissue specificity

Transcripts are highly detected in placenta, lung, followed by skeletal muscle, heart, pancreas, ovaries and kidney and weakly detected in liver and brain. May have a short half-live. In cells of hematopoietic origin, strongly and exclusively detected in terminally differentiated macrophages. Transcript expression appears to be down-regulated in primary lung tumor.

Induction

Cytokine and cellular adhesion trigger FNK induction.

Post-translational modification

Phosphorylated as cells enter mitosis and dephosphorylated as cells exit mitosis By similarity. Ref.9

Sequence similarities

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDC5/Polo subfamily.

Contains 2 POLO box domains.

Contains 1 protein kinase domain.

Ontologies

Keywords
   Cellular componentMembrane
   Coding sequence diversityPolymorphism
   DomainRepeat
   LigandATP-binding
Nucleotide-binding
   Molecular functionKinase
Serine/threonine-protein kinase
Transferase
   PTMPhosphoprotein
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processcell cycle

Inferred from electronic annotation. Source: InterPro

protein amino acid phosphorylation Ref.6

Traceable author statement. Source: ProtInc

   Cellular componentextrinsic to membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

polo kinase kinase activity

Inferred from electronic annotation. Source: InterPro

protein binding

Inferred from physical interaction. Source: IntAct

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

VRK1Q999866EBI-751877,EBI-1769146

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 646646Serine/threonine-protein kinase PLK3
PRO_0000086564

Regions

Domain62 – 314253Protein kinase
Domain470 – 53768POLO box 1
Domain567 – 63771POLO box 2
Nucleotide binding68 – 769ATP By similarity

Sites

Active site1851Proton acceptor By similarity
Binding site911ATP By similarity

Amino acid modifications

Modified residue3481Phosphothreonine Ref.9

Natural variations

Natural variant611T → S: dbSNP rs17884581. Ref.2
VAR_021091
Natural variant681L → F: dbSNP rs17884316. Ref.2
VAR_021092
Natural variant2831L → F: dbSNP rs17880471. Ref.2
VAR_021093
Natural variant4831R → C: dbSNP rs17884653. Ref.2
VAR_021094
Natural variant4911D → N: dbSNP rs17855444.
VAR_062384
Natural variant4981S → L: dbSNP rs17880829. Ref.2
VAR_021095
Natural variant6181S → P: dbSNP rs17881786. Ref.2
VAR_021096

Experimental info

Sequence conflict161A → T in CAC10659. Ref.1
Sequence conflict201P → T in CAC10659. Ref.1
Sequence conflict991A → V in CAC10659. Ref.1
Sequence conflict3531G → V in CAC10659. Ref.1
Sequence conflict4191D → H in CAC10659. Ref.1
Sequence conflict464 – 4707VSKWVDY → FSEWVGF in CAC10659. Ref.1
Sequence conflict5221P → R in CAC10659. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q9H4B4-1 [UniParc].

Last modified August 16, 2004. Version 2.
Checksum: 324CA3E9DE482514

FASTA64671,629
        10         20         30         40         50         60 
MEPAAGFLSP RPFQRAAAAP APPAGPGPPP SALRGPELEM LAGLPTSDPG RLITDPRSGR 

        70         80         90        100        110        120 
TYLKGRLLGK GGFARCYEAT DTETGSAYAV KVIPQSRVAK PHQREKILNE IELHRDLQHR 

       130        140        150        160        170        180 
HIVRFSHHFE DADNIYIFLE LCSRKSLAHI WKARHTLLEP EVRYYLRQIL SGLKYLHQRG 

       190        200        210        220        230        240 
ILHRDLKLGN FFITENMELK VGDFGLAARL EPPEQRKKTI CGTPNYVAPE VLLRQGHGPE 

       250        260        270        280        290        300 
ADVWSLGCVM YTLLCGSPPF ETADLKETYR CIKQVHYTLP ASLSLPARQL LAAILRASPR 

       310        320        330        340        350        360 
DRPSIDQILR HDFFTKGYTP DRLPISSCVT VPDLTPPNPA RSLFAKVTKS LFGRKKKSKN 

       370        380        390        400        410        420 
HAQERDEVSG LVSGLMRTSV GHQDARPEAP AASGPAPVSL VETAPEDSSP RGTLASSGDG 

       430        440        450        460        470        480 
FEEGLTVATV VESALCALRN CIAFMPPAEQ NPAPLAQPEP LVWVSKWVDY SNKFGFGYQL 

       490        500        510        520        530        540 
SSRRVAVLFN DGTHMALSAN RKTVHYNPTS TKHFSFSVGA VPRALQPQLG ILRYFASYME 

       550        560        570        580        590        600 
QHLMKGGDLP SVEEVEVPAP PLLLQWVKTD QALLMLFSDG TVQVNFYGDH TKLILSGWEP 

       610        620        630        640 
LLVTFVARNR SACTYLASHL RQLGCSPDLR QRLRYALRLL RDRSPA 

« Hide

References

« Hide 'large scale' references
[1]"Adhesion induced expression of the serine/threonine kinase Fnk in human macrophages."
Holtrich U., Wolf G., Yuan J., Bereiter-Hahn J., Karn T., Weiler M., Kauselmann G., Rehli M., Andreesen R., Kaufmann M., Kuhl D., Strebhardt K.
Oncogene 19:4832-4839(2000) [PubMed: 11039900] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Embryo.
[2]NIEHS SNPs program
Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS SER-61; PHE-68; PHE-283; CYS-483; LEU-498 AND PRO-618.
[3]"The DNA sequence and biological annotation of human chromosome 1."
Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. expand/collapse author list , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.
Nature 441:315-321(2006) [PubMed: 16710414] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT ASN-491.
Tissue: Brain.
[6]"Prk, a cytokine-inducible human protein serine/threonine kinase whose expression appears to be down-regulated in lung carcinomas."
Li B., Ouyang B., Pan H., Reissmann P.T., Slamon D.J., Arceci R., Lu L., Dai W.
J. Biol. Chem. 271:19402-19408(1996) [PubMed: 8702627] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 28-646.
Tissue: Placenta.
[7]"Human Prk is a conserved protein serine/threonine kinase involved in regulating M phase functions."
Ouyang B., Pan H., Lu L., Li J., Stambrook P., Li B., Dai W.
J. Biol. Chem. 272:28646-28651(1997) [PubMed: 9353331] [Abstract]
Cited for: CHARACTERIZATION.
[8]"The physical association and phosphorylation of Cdc25C protein phosphatase by Prk."
Ouyang B., Li W., Pan H., Meadows J., Hoffmann I., Dai W.
Oncogene 18:6029-6036(1999) [PubMed: 10557092] [Abstract]
Cited for: CHARACTERIZATION.
[9]"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
Anal. Chem. 81:4493-4501(2009) [PubMed: 19413330] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-348, MASS SPECTROMETRY.
+Additional computationally mapped references.

Web resources

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ293866 mRNA. Translation: CAC10659.1.
AY764184 Genomic DNA. Translation: AAU88146.1.
AL592166 Genomic DNA. Translation: CAI13006.1.
CH471059 Genomic DNA. Translation: EAX07021.1.
BC013899 mRNA. Translation: AAH13899.1.
U56998 mRNA. Translation: AAC50637.1. Different initiation.
IPIIPI00304186.
RefSeqNP_004064.2.
UniGeneHs.632415

3D structure databases

SMRQ9H4B4. Positions 52-329, 458-642.
ModBaseSearch...

Protein-protein interaction databases

IntActQ9H4B4. 3 interactions.
STRINGQ9H4B4.

Proteomic databases

PRIDEQ9H4B4.

Genome annotation databases

EnsemblENST00000372201; ENSP00000361275; ENSG00000173846; Homo sapiens. [Genome view]
GeneID1263.
KEGGhsa:1263.
UCSCuc001cmn.1. human.

Organism-specific databases

CTD1263.
GeneCardsGC01P045038.
H-InvDBHIX0000520.
HGNCHGNC:2154. PLK3.
MIM602913. gene.
PharmGKBPA26664.
GenAtlasSearch...

Phylogenomic databases

eggNOGprNOG10409.
HOGENOMHBG738952.
HOVERGENQ9H4B4.
InParanoidQ9H4B4.
OMAPRSGRTY.
OrthoDBEOG9NW252.
PhylomeDBQ9H4B4.

Enzyme and pathway databases

BRENDA2.7.11.21. 247.

Gene expression databases

ArrayExpressQ9H4B4.
BgeeQ9H4B4.
CleanExHS_PLK3.
GenevestigatorQ9H4B4.
GermOnlineENSG00000173846. Homo sapiens.

Family and domain databases

InterProIPR020636. Ca/CaM-dep_prot_kinase-like.
IPR011009. Kinase-like_dom.
IPR000959. POLO_box_duplicated_dom.
IPR000719. Prot_kinase_cat_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR017442. Se/Thr_prot_kinase-like_dom.
IPR008271. Ser/Thr_prot_kinase_AS.
IPR002290. Ser/Thr_prot_kinase_dom.
IPR015728. Ser/Thr_prot_kinase_Polo-like.
[Graphical view]
PANTHERPTHR22982. Ca/CaM-dep_prot_kinase-like. 1 hit.
PTHR22982:SF10. Polo-like_kinase. 1 hit.
PfamPF00069. Pkinase. 1 hit.
PF00659. POLO_box. 2 hits.
[Graphical view]
SMARTSM00220. S_TKc. 1 hit.
[Graphical view]
PROSITEPS50078. POLO_BOX. 2 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio5111.
SOURCESearch...

Entry information

Entry namePLK3_HUMAN
AccessionPrimary (citable) accession number: Q9H4B4
Secondary accession number(s): Q15767, Q5JR99, Q96CV1
Entry history
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: August 16, 2004
Last modified: February 9, 2010
This is version 92 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

Human chromosome 1

Human chromosome 1: entries, gene names and cross-references to MIM

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human and mouse protein kinases

Human and mouse protein kinases: classification and index

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Web resources · Cross-references · Entry information · Relevant documents