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Protein

Golgi phosphoprotein 3

Gene

GOLPH3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Phosphatidylinositol-4-phosphate-binding protein that links Golgi membranes to the cytoskeleton and may participate in the tensile force required for vesicle budding from the Golgi. Thereby, may play a role in Golgi membrane trafficking and could indirectly give its flattened shape to the Golgi apparatus. May also bind to the coatomer to regulate Golgi membrane trafficking. May play a role in anterograde transport from the Golgi to the plasma membrane and regulate secretion. Has also been involved in the control of the localization of Golgi enzymes through interaction with their cytoplasmic part. May play an indirect role in cell migration. Has also been involved in the modulation of mTOR signaling. May also be involved in the regulation of mitochondrial lipids biosynthesis.7 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei81PtdIns4PCurated1
Binding sitei90PtdIns4PCurated1
Binding sitei171PtdIns4PCurated1
Binding sitei174PtdIns4PCurated1

GO - Molecular functioni

  • enzyme binding Source: UniProtKB
  • phosphatidylinositol-4-phosphate binding Source: UniProtKB

GO - Biological processi

  • asymmetric Golgi ribbon formation Source: CACAO
  • cell adhesion molecule production Source: UniProtKB
  • cell migration Source: UniProtKB
  • cell proliferation Source: UniProtKB
  • gene expression Source: UniProtKB
  • glycoprotein biosynthetic process Source: UniProtKB
  • Golgi organization Source: UniProtKB
  • Golgi ribbon formation Source: CACAO
  • Golgi to plasma membrane protein transport Source: UniProtKB
  • Golgi vesicle budding Source: UniProtKB
  • lamellipodium assembly Source: UniProtKB
  • leukocyte tethering or rolling Source: UniProtKB
  • negative regulation of apoptotic process Source: CACAO
  • positive regulation of protein secretion Source: UniProtKB
  • positive regulation of TOR signaling Source: UniProtKB
  • protein retention in Golgi apparatus Source: UniProtKB
  • protein secretion Source: UniProtKB
  • regulation of mitochondrion organization Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Keywords - Ligandi

Lipid-binding

Enzyme and pathway databases

BioCyciZFISH:ENSG00000113384-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Golgi phosphoprotein 3
Alternative name(s):
Coat protein GPP34
Mitochondrial DNA absence factor
Short name:
MIDAS
Gene namesi
Name:GOLPH3
Synonyms:GPP34
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 5

Organism-specific databases

HGNCiHGNC:15452. GOLPH3.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: UniProtKB
  • endosome Source: UniProtKB-SubCell
  • Golgi apparatus Source: CACAO
  • Golgi cisterna Source: UniProtKB
  • Golgi cisterna membrane Source: UniProtKB-SubCell
  • intracellular membrane-bounded organelle Source: HPA
  • mitochondrial intermembrane space Source: UniProtKB-SubCell
  • mitochondrion Source: UniProtKB
  • nucleoplasm Source: HPA
  • plasma membrane Source: UniProtKB-SubCell
  • trans-Golgi network Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Endosome, Golgi apparatus, Membrane, Mitochondrion

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi7R → A: Altered binding to coatomer. 1 Publication1
Mutagenesisi14 – 15RR → AA: Loss of binding to coatomer. 1 Publication2
Mutagenesisi81W → A: Abolishes phosphoinositide binding and localization to the Golgi apparatus; when associated with A-90. 1 Publication1
Mutagenesisi90R → A: Abolishes phosphoinositide binding and localization to the Golgi apparatus; when associated with A-81. 2 Publications1
Mutagenesisi90R → L: Loss of function in vesicle budding, abolishes phosphoinositide binding and localization to the Golgi apparatus. 2 Publications1
Mutagenesisi171R → A or L: Abolishes phosphoinositide binding and localization to the Golgi apparatus; when associated with A-174. 2 Publications1
Mutagenesisi174R → A: Abolishes phosphoinositide binding and localization to the Golgi apparatus; when associated with A-171 or L-171. 2 Publications1

Organism-specific databases

DisGeNETi64083.
OpenTargetsiENSG00000113384.
PharmGKBiPA28812.

Polymorphism and mutation databases

BioMutaiGOLPH3.
DMDMi50400651.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001238191 – 298Golgi phosphoprotein 3Add BLAST298

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei35PhosphoserineCombined sources1
Modified residuei36PhosphoserineCombined sources1
Disulfide bondi84 ↔ 1081 Publication

Post-translational modificationi

Phosphorylated.By similarity

Keywords - PTMi

Disulfide bond, Phosphoprotein

Proteomic databases

EPDiQ9H4A6.
MaxQBiQ9H4A6.
PaxDbiQ9H4A6.
PeptideAtlasiQ9H4A6.
PRIDEiQ9H4A6.

PTM databases

iPTMnetiQ9H4A6.
PhosphoSitePlusiQ9H4A6.

Expressioni

Tissue specificityi

Detected in muscle fibers of patients with mitochondrial diseases; not detected in normal muscle fibers.

Inductioni

Activated by depletion of mitochondrial DNA.

Gene expression databases

BgeeiENSG00000113384.
CleanExiHS_GOLPH3.
ExpressionAtlasiQ9H4A6. baseline and differential.
GenevisibleiQ9H4A6. HS.

Organism-specific databases

HPAiHPA044564.
HPA055841.

Interactioni

Subunit structurei

Homodimer. Interacts with the coatomer complex. Interacts with MYO18A; the interaction is direct and may link Golgi membranes to the actin cytoskeleton. Interacts with GCNT1; may control its retention in the Golgi. Interacts with VPS35.5 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
ARL6IP1Q150413EBI-2465479,EBI-714543
GCNT1Q027426EBI-2465479,EBI-8766035
MYO18AQ926146EBI-2465479,EBI-949059
vprP125203EBI-2465479,EBI-6164519From a different organism.
XRCC5P130102EBI-2465479,EBI-357997

GO - Molecular functioni

  • enzyme binding Source: UniProtKB

Protein-protein interaction databases

BioGridi122048. 40 interactors.
IntActiQ9H4A6. 9 interactors.
MINTiMINT-5001986.
STRINGi9606.ENSP00000265070.

Structurei

Secondary structure

1298
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi62 – 69Combined sources8
Beta strandi73 – 75Combined sources3
Helixi83 – 99Combined sources17
Beta strandi102 – 105Combined sources4
Turni110 – 112Combined sources3
Turni115 – 117Combined sources3
Beta strandi118 – 123Combined sources6
Helixi131 – 142Combined sources12
Helixi149 – 156Combined sources8
Helixi163 – 166Combined sources4
Helixi173 – 183Combined sources11
Beta strandi190 – 194Combined sources5
Beta strandi197 – 201Combined sources5
Helixi207 – 220Combined sources14
Turni221 – 224Combined sources4
Helixi229 – 231Combined sources3
Helixi234 – 245Combined sources12
Helixi250 – 253Combined sources4
Helixi258 – 273Combined sources16
Helixi276 – 279Combined sources4
Beta strandi282 – 284Combined sources3
Helixi287 – 295Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3KN1X-ray2.90A52-298[»]
ProteinModelPortaliQ9H4A6.
SMRiQ9H4A6.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9H4A6.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni190 – 201Beta-hairpin required for oligomerizationBy similarityAdd BLAST12

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi48 – 51Poly-Asp4

Sequence similaritiesi

Belongs to the GOLPH3/VPS74 family.Curated

Phylogenomic databases

eggNOGiKOG3983. Eukaryota.
ENOG410XRT6. LUCA.
GeneTreeiENSGT00390000007153.
HOGENOMiHOG000209848.
HOVERGENiHBG059607.
InParanoidiQ9H4A6.
KOiK15620.
OMAiESMKANT.
OrthoDBiEOG091G0M99.
PhylomeDBiQ9H4A6.
TreeFamiTF314360.

Family and domain databases

InterProiIPR008628. GPP34.
[Graphical view]
PfamiPF05719. GPP34. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9H4A6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTSLTQRSSG LVQRRTEASR NAADKERAAG GGAGSSEDDA QSRRDEQDDD
60 70 80 90 100
DKGDSKETRL TLMEEVLLLG LKDREGYTSF WNDCISSGLR GCMLIELALR
110 120 130 140 150
GRLQLEACGM RRKSLLTRKV ICKSDAPTGD VLLDEALKHV KETQPPETVQ
160 170 180 190 200
NWIELLSGET WNPLKLHYQL RNVRERLAKN LVEKGVLTTE KQNFLLFDMT
210 220 230 240 250
THPLTNNNIK QRLIKKVQEA VLDKWVNDPH RMDRRLLALI YLAHASDVLE
260 270 280 290
NAFAPLLDEQ YDLATKRVRQ LLDLDPEVEC LKANTNEVLW AVVAAFTK
Length:298
Mass (Da):33,811
Last modified:March 1, 2001 - v1
Checksum:i34FAD78155CC214A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ296152 mRNA. Translation: CAC13124.1.
AK075156 mRNA. Translation: BAC11438.1.
AL356292 Genomic DNA. No translation available.
AL356356 Genomic DNA. No translation available.
BC012123 mRNA. Translation: AAH12123.1.
BC033725 mRNA. Translation: AAH33725.1.
BC063586 mRNA. Translation: AAH63586.1.
AL133078 mRNA. Translation: CAB61398.1.
CCDSiCCDS3896.1.
PIRiT42677.
RefSeqiNP_071413.1. NM_022130.3.
UniGeneiHs.408909.

Genome annotation databases

EnsembliENST00000265070; ENSP00000265070; ENSG00000113384.
GeneIDi64083.
KEGGihsa:64083.
UCSCiuc003jhp.2. human.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ296152 mRNA. Translation: CAC13124.1.
AK075156 mRNA. Translation: BAC11438.1.
AL356292 Genomic DNA. No translation available.
AL356356 Genomic DNA. No translation available.
BC012123 mRNA. Translation: AAH12123.1.
BC033725 mRNA. Translation: AAH33725.1.
BC063586 mRNA. Translation: AAH63586.1.
AL133078 mRNA. Translation: CAB61398.1.
CCDSiCCDS3896.1.
PIRiT42677.
RefSeqiNP_071413.1. NM_022130.3.
UniGeneiHs.408909.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3KN1X-ray2.90A52-298[»]
ProteinModelPortaliQ9H4A6.
SMRiQ9H4A6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi122048. 40 interactors.
IntActiQ9H4A6. 9 interactors.
MINTiMINT-5001986.
STRINGi9606.ENSP00000265070.

PTM databases

iPTMnetiQ9H4A6.
PhosphoSitePlusiQ9H4A6.

Polymorphism and mutation databases

BioMutaiGOLPH3.
DMDMi50400651.

Proteomic databases

EPDiQ9H4A6.
MaxQBiQ9H4A6.
PaxDbiQ9H4A6.
PeptideAtlasiQ9H4A6.
PRIDEiQ9H4A6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000265070; ENSP00000265070; ENSG00000113384.
GeneIDi64083.
KEGGihsa:64083.
UCSCiuc003jhp.2. human.

Organism-specific databases

CTDi64083.
DisGeNETi64083.
GeneCardsiGOLPH3.
H-InvDBHIX0032097.
HGNCiHGNC:15452. GOLPH3.
HPAiHPA044564.
HPA055841.
MIMi612207. gene.
neXtProtiNX_Q9H4A6.
OpenTargetsiENSG00000113384.
PharmGKBiPA28812.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3983. Eukaryota.
ENOG410XRT6. LUCA.
GeneTreeiENSGT00390000007153.
HOGENOMiHOG000209848.
HOVERGENiHBG059607.
InParanoidiQ9H4A6.
KOiK15620.
OMAiESMKANT.
OrthoDBiEOG091G0M99.
PhylomeDBiQ9H4A6.
TreeFamiTF314360.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000113384-MONOMER.

Miscellaneous databases

ChiTaRSiGOLPH3. human.
EvolutionaryTraceiQ9H4A6.
GenomeRNAii64083.
PROiQ9H4A6.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000113384.
CleanExiHS_GOLPH3.
ExpressionAtlasiQ9H4A6. baseline and differential.
GenevisibleiQ9H4A6. HS.

Family and domain databases

InterProiIPR008628. GPP34.
[Graphical view]
PfamiPF05719. GPP34. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGOLP3_HUMAN
AccessioniPrimary (citable) accession number: Q9H4A6
Secondary accession number(s): Q9UIW5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: March 1, 2001
Last modified: November 2, 2016
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Miscellaneous

Modulates sensitivity to rapamycin. Tumors expressing this protein are more sensitive to rapamycin in vivo.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 5
    Human chromosome 5: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.