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Q9H4A5

- GLP3L_HUMAN

UniProt

Q9H4A5 - GLP3L_HUMAN

Protein

Golgi phosphoprotein 3-like

Gene

GOLPH3L

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 99 (01 Oct 2014)
      Sequence version 1 (01 Mar 2001)
      Previous versions | rss
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    Functioni

    Phosphatidylinositol-4-phosphate-binding protein that may antagonize the action of GOLPH3 which is required for the process of vesicle budding at the Golgi and anterograde transport to the plasma membrane.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei67 – 671PtdIns4PBy similarity
    Binding sitei76 – 761PtdIns4PBy similarity
    Binding sitei157 – 1571PtdIns4PBy similarity
    Binding sitei160 – 1601PtdIns4PBy similarity

    GO - Molecular functioni

    1. phosphatidylinositol-4-phosphate binding Source: UniProtKB
    2. protein binding Source: IntAct

    GO - Biological processi

    1. Golgi organization Source: UniProtKB
    2. positive regulation of protein secretion Source: UniProtKB

    Keywords - Ligandi

    Lipid-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Golgi phosphoprotein 3-like
    Alternative name(s):
    GPP34-related protein
    Gene namesi
    Name:GOLPH3L
    Synonyms:GPP34R
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 1

    Organism-specific databases

    HGNCiHGNC:24882. GOLPH3L.

    Subcellular locationi

    Golgi apparatusGolgi stack membrane 1 Publication; Peripheral membrane protein 1 Publication; Cytoplasmic side 1 Publication. Golgi apparatustrans-Golgi network membrane 1 Publication; Peripheral membrane protein 1 Publication; Cytoplasmic side 1 Publication
    Note: Phosphatidylinositol 4-phosphate (PtdIns4P)-binding mediates recruitment to Golgi membranes.

    GO - Cellular componenti

    1. Golgi cisterna membrane Source: UniProtKB-SubCell
    2. Golgi membrane Source: UniProtKB
    3. trans-Golgi network membrane Source: UniProtKB

    Keywords - Cellular componenti

    Golgi apparatus, Membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi7 – 104RARR → AAAA: Loss of binding to coatomer. 1 Publication

    Organism-specific databases

    PharmGKBiPA134922683.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 285285Golgi phosphoprotein 3-likePRO_0000324135Add
    BLAST

    Proteomic databases

    MaxQBiQ9H4A5.
    PaxDbiQ9H4A5.
    PeptideAtlasiQ9H4A5.
    PRIDEiQ9H4A5.

    PTM databases

    PhosphoSiteiQ9H4A5.

    Expressioni

    Gene expression databases

    ArrayExpressiQ9H4A5.
    BgeeiQ9H4A5.
    CleanExiHS_GOLPH3L.
    GenevestigatoriQ9H4A5.

    Organism-specific databases

    HPAiHPA028558.

    Interactioni

    Subunit structurei

    Homooligomer By similarity. Does not interact MYO18; differs from GOLPH3 by its inability to interact with MYO18. May interact with ARF1.By similarity

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    HTTP428582EBI-4403434,EBI-466029
    ZDHHC17Q8IUH52EBI-4403434,EBI-524753

    Protein-protein interaction databases

    BioGridi120500. 4 interactions.
    IntActiQ9H4A5. 3 interactions.
    STRINGi9606.ENSP00000271732.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9H4A5.
    SMRiQ9H4A5. Positions 45-282.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni176 – 18712Beta-hairpin required for oligomerizationBy similarityAdd
    BLAST

    Sequence similaritiesi

    Belongs to the GOLPH3/VPS74 family.Curated

    Phylogenomic databases

    eggNOGiNOG79916.
    HOGENOMiHOG000209848.
    HOVERGENiHBG059607.
    KOiK15620.
    OMAiDKYDMAM.
    OrthoDBiEOG7ZKSBV.
    PhylomeDBiQ9H4A5.
    TreeFamiTF314360.

    Family and domain databases

    InterProiIPR008628. GPP34.
    [Graphical view]
    PANTHERiPTHR12704. PTHR12704. 1 hit.
    PfamiPF05719. GPP34. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q9H4A5-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MTTLTHRARR TEISKNSEKK MESEEDSNWE KSPDNEDSGD SKDIRLTLME    50
    EVLLLGLKDK EGYTSFWNDC ISSGLRGGIL IELAMRGRIY LEPPTMRKKR 100
    LLDRKVLLKS DSPTGDVLLD ETLKHIKATE PTETVQTWIE LLTGETWNPF 150
    KLQYQLRNVR ERIAKNLVEK GILTTEKQNF LLFDMTTHPV TNTTEKQRLV 200
    KKLQDSVLER WVNDPQRMDK RTLALLVLAH SSDVLENVFS SLTDDKYDVA 250
    MNRAKDLVEL DPEVEGTKPS ATEMIWAVLA AFNKS 285
    Length:285
    Mass (Da):32,767
    Last modified:March 1, 2001 - v1
    Checksum:iE618915FD86F3772
    GO
    Isoform 2 (identifier: Q9H4A5-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         62-105: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:241
    Mass (Da):27,672
    Checksum:iEB4C9E1203CEEAEA
    GO

    Sequence cautioni

    The sequence BAA91750.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti112 – 1121S → G in BAH13319. (PubMed:14702039)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei62 – 10544Missing in isoform 2. 1 PublicationVSP_055229Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ296153 mRNA. Translation: CAC13125.1.
    AK001549 mRNA. Translation: BAA91750.1. Different initiation.
    AK300635 mRNA. Translation: BAH13319.1.
    AK315594 mRNA. Translation: BAG37966.1.
    AL356292, AL356356 Genomic DNA. Translation: CAI13650.1.
    AL356356, AL356292 Genomic DNA. Translation: CAI15495.1.
    CH471121 Genomic DNA. Translation: EAW53526.1.
    BC013870 mRNA. Translation: AAH13870.1.
    CCDSiCCDS966.1. [Q9H4A5-1]
    RefSeqiNP_060648.2. NM_018178.5.
    UniGeneiHs.203699.

    Genome annotation databases

    EnsembliENST00000271732; ENSP00000271732; ENSG00000143457. [Q9H4A5-1]
    GeneIDi55204.
    KEGGihsa:55204.
    UCSCiuc001evj.2. human. [Q9H4A5-1]

    Polymorphism databases

    DMDMi74752638.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ296153 mRNA. Translation: CAC13125.1 .
    AK001549 mRNA. Translation: BAA91750.1 . Different initiation.
    AK300635 mRNA. Translation: BAH13319.1 .
    AK315594 mRNA. Translation: BAG37966.1 .
    AL356292 , AL356356 Genomic DNA. Translation: CAI13650.1 .
    AL356356 , AL356292 Genomic DNA. Translation: CAI15495.1 .
    CH471121 Genomic DNA. Translation: EAW53526.1 .
    BC013870 mRNA. Translation: AAH13870.1 .
    CCDSi CCDS966.1. [Q9H4A5-1 ]
    RefSeqi NP_060648.2. NM_018178.5.
    UniGenei Hs.203699.

    3D structure databases

    ProteinModelPortali Q9H4A5.
    SMRi Q9H4A5. Positions 45-282.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 120500. 4 interactions.
    IntActi Q9H4A5. 3 interactions.
    STRINGi 9606.ENSP00000271732.

    PTM databases

    PhosphoSitei Q9H4A5.

    Polymorphism databases

    DMDMi 74752638.

    Proteomic databases

    MaxQBi Q9H4A5.
    PaxDbi Q9H4A5.
    PeptideAtlasi Q9H4A5.
    PRIDEi Q9H4A5.

    Protocols and materials databases

    DNASUi 55204.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000271732 ; ENSP00000271732 ; ENSG00000143457 . [Q9H4A5-1 ]
    GeneIDi 55204.
    KEGGi hsa:55204.
    UCSCi uc001evj.2. human. [Q9H4A5-1 ]

    Organism-specific databases

    CTDi 55204.
    GeneCardsi GC01M150618.
    HGNCi HGNC:24882. GOLPH3L.
    HPAi HPA028558.
    MIMi 612208. gene.
    neXtProti NX_Q9H4A5.
    PharmGKBi PA134922683.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG79916.
    HOGENOMi HOG000209848.
    HOVERGENi HBG059607.
    KOi K15620.
    OMAi DKYDMAM.
    OrthoDBi EOG7ZKSBV.
    PhylomeDBi Q9H4A5.
    TreeFami TF314360.

    Miscellaneous databases

    ChiTaRSi GOLPH3L. human.
    GenomeRNAii 55204.
    NextBioi 35480017.
    PROi Q9H4A5.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q9H4A5.
    Bgeei Q9H4A5.
    CleanExi HS_GOLPH3L.
    Genevestigatori Q9H4A5.

    Family and domain databases

    InterProi IPR008628. GPP34.
    [Graphical view ]
    PANTHERi PTHR12704. PTHR12704. 1 hit.
    Pfami PF05719. GPP34. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
      Tissue: Placenta.
    3. "The DNA sequence and biological annotation of human chromosome 1."
      Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K.
      , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.
      Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Ovary.
    6. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    7. "A conserved N-terminal arginine-motif in GOLPH3-family proteins mediates binding to coatomer."
      Tu L., Chen L., Banfield D.K.
      Traffic 13:1496-1507(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: MUTAGENESIS OF 7-ARG--ARG-10, COATOMER-BINDING, INTERACTION WITH ARF1.
    8. "GOLPH3L antagonizes GOLPH3 to determine Golgi morphology."
      Ng M.M., Dippold H.C., Buschman M.D., Noakes C.J., Field S.J.
      Mol. Biol. Cell 24:796-808(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, LIPID-BINDING, SUBCELLULAR LOCATION.

    Entry informationi

    Entry nameiGLP3L_HUMAN
    AccessioniPrimary (citable) accession number: Q9H4A5
    Secondary accession number(s): B1AN09, B7Z6N3, Q9NVK0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 18, 2008
    Last sequence update: March 1, 2001
    Last modified: October 1, 2014
    This is version 99 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 1
      Human chromosome 1: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3