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Q9H4A5 (GLP3L_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 97. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Golgi phosphoprotein 3-like
Alternative name(s):
GPP34-related protein
Gene names
Name:GOLPH3L
Synonyms:GPP34R
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length285 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Phosphatidylinositol-4-phosphate-binding protein that may antagonize the action of GOLPH3 which is required for the process of vesicle budding at the Golgi and anterograde transport to the plasma membrane. Ref.8

Subunit structure

Homooligomer By similarity. Does not interact MYO18; differs from GOLPH3 by its inability to interact with MYO18. May interact with ARF1.

Subcellular location

Golgi apparatusGolgi stack membrane; Peripheral membrane protein; Cytoplasmic side. Golgi apparatustrans-Golgi network membrane; Peripheral membrane protein; Cytoplasmic side. Note: Phosphatidylinositol 4-phosphate (PtdIns4P)-binding mediates recruitment to Golgi membranes. Ref.8

Sequence similarities

Belongs to the GOLPH3/VPS74 family.

Sequence caution

The sequence BAA91750.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Binary interactions

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9H4A5-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9H4A5-2)

The sequence of this isoform differs from the canonical sequence as follows:
     62-105: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 285285Golgi phosphoprotein 3-like
PRO_0000324135

Regions

Region176 – 18712Beta-hairpin required for oligomerization By similarity

Sites

Binding site671PtdIns4P By similarity
Binding site761PtdIns4P By similarity
Binding site1571PtdIns4P By similarity
Binding site1601PtdIns4P By similarity

Natural variations

Alternative sequence62 – 10544Missing in isoform 2.
VSP_055229

Experimental info

Mutagenesis7 – 104RARR → AAAA: Loss of binding to coatomer. Ref.7
Sequence conflict1121S → G in BAH13319. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified March 1, 2001. Version 1.
Checksum: E618915FD86F3772

FASTA28532,767
        10         20         30         40         50         60 
MTTLTHRARR TEISKNSEKK MESEEDSNWE KSPDNEDSGD SKDIRLTLME EVLLLGLKDK 

        70         80         90        100        110        120 
EGYTSFWNDC ISSGLRGGIL IELAMRGRIY LEPPTMRKKR LLDRKVLLKS DSPTGDVLLD 

       130        140        150        160        170        180 
ETLKHIKATE PTETVQTWIE LLTGETWNPF KLQYQLRNVR ERIAKNLVEK GILTTEKQNF 

       190        200        210        220        230        240 
LLFDMTTHPV TNTTEKQRLV KKLQDSVLER WVNDPQRMDK RTLALLVLAH SSDVLENVFS 

       250        260        270        280 
SLTDDKYDVA MNRAKDLVEL DPEVEGTKPS ATEMIWAVLA AFNKS 

« Hide

Isoform 2 [UniParc].

Checksum: EB4C9E1203CEEAEA
Show »

FASTA24127,672

References

« Hide 'large scale' references
[1]"Proteomics characterization of abundant Golgi membrane proteins."
Bell A.W., Ward M.A., Blackstock W.P., Freeman H.N.M., Choudhary J.S., Lewis A.P., Chotai D., Fazel A., Gushue J.N., Paiement J., Palcy S., Chevet E., Lafreniere-Roula M., Solari R., Thomas D.Y., Rowley A., Bergeron J.J.M.
J. Biol. Chem. 276:5152-5165(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Tissue: Placenta.
[3]"The DNA sequence and biological annotation of human chromosome 1."
Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. expand/collapse author list , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.
Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Ovary.
[6]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[7]"A conserved N-terminal arginine-motif in GOLPH3-family proteins mediates binding to coatomer."
Tu L., Chen L., Banfield D.K.
Traffic 13:1496-1507(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: MUTAGENESIS OF 7-ARG--ARG-10, COATOMER-BINDING, INTERACTION WITH ARF1.
[8]"GOLPH3L antagonizes GOLPH3 to determine Golgi morphology."
Ng M.M., Dippold H.C., Buschman M.D., Noakes C.J., Field S.J.
Mol. Biol. Cell 24:796-808(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, LIPID-BINDING, SUBCELLULAR LOCATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ296153 mRNA. Translation: CAC13125.1.
AK001549 mRNA. Translation: BAA91750.1. Different initiation.
AK300635 mRNA. Translation: BAH13319.1.
AK315594 mRNA. Translation: BAG37966.1.
AL356292, AL356356 Genomic DNA. Translation: CAI13650.1.
AL356356, AL356292 Genomic DNA. Translation: CAI15495.1.
CH471121 Genomic DNA. Translation: EAW53526.1.
BC013870 mRNA. Translation: AAH13870.1.
CCDSCCDS966.1.
RefSeqNP_060648.2. NM_018178.5.
UniGeneHs.203699.

3D structure databases

ProteinModelPortalQ9H4A5.
SMRQ9H4A5. Positions 45-282.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid120500. 4 interactions.
IntActQ9H4A5. 3 interactions.
STRING9606.ENSP00000271732.

PTM databases

PhosphoSiteQ9H4A5.

Polymorphism databases

DMDM74752638.

Proteomic databases

MaxQBQ9H4A5.
PaxDbQ9H4A5.
PeptideAtlasQ9H4A5.
PRIDEQ9H4A5.

Protocols and materials databases

DNASU55204.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000271732; ENSP00000271732; ENSG00000143457.
GeneID55204.
KEGGhsa:55204.
UCSCuc001evj.2. human.

Organism-specific databases

CTD55204.
GeneCardsGC01M150618.
HGNCHGNC:24882. GOLPH3L.
HPAHPA028558.
MIM612208. gene.
neXtProtNX_Q9H4A5.
PharmGKBPA134922683.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG79916.
HOGENOMHOG000209848.
HOVERGENHBG059607.
KOK15620.
OMADKYDMAM.
OrthoDBEOG7ZKSBV.
PhylomeDBQ9H4A5.
TreeFamTF314360.

Gene expression databases

ArrayExpressQ9H4A5.
BgeeQ9H4A5.
CleanExHS_GOLPH3L.
GenevestigatorQ9H4A5.

Family and domain databases

InterProIPR008628. GPP34.
[Graphical view]
PANTHERPTHR12704. PTHR12704. 1 hit.
PfamPF05719. GPP34. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSGOLPH3L. human.
GenomeRNAi55204.
NextBio59098.
PROQ9H4A5.
SOURCESearch...

Entry information

Entry nameGLP3L_HUMAN
AccessionPrimary (citable) accession number: Q9H4A5
Secondary accession number(s): B1AN09, B7Z6N3, Q9NVK0
Entry history
Integrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: March 1, 2001
Last modified: July 9, 2014
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 1

Human chromosome 1: entries, gene names and cross-references to MIM