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Protein

Aminopeptidase B

Gene

RNPEP

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Exopeptidase which selectively removes arginine and/or lysine residues from the N-terminus of several peptide substrates including Arg0-Leu-enkephalin, Arg0-Met-enkephalin and Arg(-1)-Lys0-somatostatin-14. Can hydrolyze leukotriene A4 (LTA-4) into leukotriene B4 (LTB-4) (By similarity).By similarity

Catalytic activityi

Release of N-terminal Arg and Lys from oligopeptides when P1' is not Pro. Also acts on arylamides of Arg and Lys.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi325Zinc; catalyticPROSITE-ProRule annotation1
Active sitei326Proton acceptorPROSITE-ProRule annotation1
Metal bindingi329Zinc; catalyticPROSITE-ProRule annotation1
Metal bindingi348Zinc; catalyticPROSITE-ProRule annotation1
Sitei414Transition state stabilizerBy similarity1

GO - Molecular functioni

  • aminopeptidase activity Source: UniProtKB
  • epoxide hydrolase activity Source: UniProtKB
  • metalloaminopeptidase activity Source: GO_Central
  • metalloexopeptidase activity Source: UniProtKB
  • peptide binding Source: GO_Central
  • zinc ion binding Source: GO_Central

GO - Biological processi

Keywordsi

Molecular functionAminopeptidase, Hydrolase, Metalloprotease, Protease
LigandMetal-binding, Zinc

Protein family/group databases

MEROPSiM01.014.

Names & Taxonomyi

Protein namesi
Recommended name:
Aminopeptidase B (EC:3.4.11.6)
Short name:
AP-B
Alternative name(s):
Arginine aminopeptidase
Arginyl aminopeptidase
Gene namesi
Name:RNPEP
Synonyms:APB
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

EuPathDBiHostDB:ENSG00000176393.10.
HGNCiHGNC:10078. RNPEP.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Organism-specific databases

DisGeNETi6051.
OpenTargetsiENSG00000176393.
PharmGKBiPA34451.

Chemistry databases

ChEMBLiCHEMBL2432.

Polymorphism and mutation databases

BioMutaiRNPEP.
DMDMi20137480.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00000950882 – 650Aminopeptidase BAdd BLAST649

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1
Modified residuei7PhosphoserineCombined sources1
Modified residuei446N6-acetyllysineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9H4A4.
MaxQBiQ9H4A4.
PaxDbiQ9H4A4.
PeptideAtlasiQ9H4A4.
PRIDEiQ9H4A4.
TopDownProteomicsiQ9H4A4.

PTM databases

iPTMnetiQ9H4A4.
PhosphoSitePlusiQ9H4A4.
SwissPalmiQ9H4A4.

Expressioni

Gene expression databases

BgeeiENSG00000176393.
CleanExiHS_RNPEP.
ExpressionAtlasiQ9H4A4. baseline and differential.
GenevisibleiQ9H4A4. HS.

Organism-specific databases

HPAiHPA036074.
HPA036075.
HPA061487.

Interactioni

Protein-protein interaction databases

BioGridi111978. 7 interactors.
IntActiQ9H4A4. 2 interactors.
MINTiMINT-1182309.
STRINGi9606.ENSP00000295640.

Chemistry databases

BindingDBiQ9H4A4.

Structurei

3D structure databases

ProteinModelPortaliQ9H4A4.
SMRiQ9H4A4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni298 – 302Substrate bindingBy similarity5

Sequence similaritiesi

Belongs to the peptidase M1 family.Curated

Phylogenomic databases

eggNOGiKOG1047. Eukaryota.
COG0308. LUCA.
GeneTreeiENSGT00530000063003.
HOVERGENiHBG001274.
InParanoidiQ9H4A4.
KOiK01260.
OMAiEGPGVCW.
OrthoDBiEOG091G02UX.
PhylomeDBiQ9H4A4.
TreeFamiTF300758.

Family and domain databases

CDDicd09599. M1_LTA4H. 1 hit.
InterProiView protein in InterPro
IPR016024. ARM-type_fold.
IPR034015. M1_LTA4H.
IPR015571. Pept_M1_aminopeptidase-B.
IPR001930. Peptidase_M1.
IPR015211. Peptidase_M1_C.
IPR014782. Peptidase_M1_N.
PANTHERiPTHR11533. PTHR11533. 1 hit.
PTHR11533:SF153. PTHR11533:SF153. 1 hit.
PfamiView protein in Pfam
PF09127. Leuk-A4-hydro_C. 1 hit.
PF01433. Peptidase_M1. 1 hit.
PRINTSiPR00756. ALADIPTASE.
SMARTiView protein in SMART
SM01263. Leuk-A4-hydro_C. 1 hit.
SUPFAMiSSF48371. SSF48371. 1 hit.
PROSITEiView protein in PROSITE
PS00142. ZINC_PROTEASE. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9H4A4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASGEHSPGS GAARRPLHSA QAVDVASASN FRAFELLHLH LDLRAEFGPP
60 70 80 90 100
GPGAGSRGLS GTAVLDLRCL EPEGAAELRL DSHPCLEVTA AALRRERPGS
110 120 130 140 150
EEPPAEPVSF YTQPFSHYGQ ALCVSFPQPC RAAERLQVLL TYRVGEGPGV
160 170 180 190 200
CWLAPEQTAG KKKPFVYTQG QAVLNRAFFP CFDTPAVKYK YSALIEVPDG
210 220 230 240 250
FTAVMSASTW EKRGPNKFFF QMCQPIPSYL IALAIGDLVS AEVGPRSRVW
260 270 280 290 300
AEPCLIDAAK EEYNGVIEEF LATGEKLFGP YVWGRYDLLF MPPSFPFGGM
310 320 330 340 350
ENPCLTFVTP CLLAGDRSLA DVIIHEISHS WFGNLVTNAN WGEFWLNEGF
360 370 380 390 400
TMYAQRRIST ILFGAAYTCL EAATGRALLR QHMDITGEEN PLNKLRVKIE
410 420 430 440 450
PGVDPDDTYN ETPYEKGFCF VSYLAHLVGD QDQFDSFLKA YVHEFKFRSI
460 470 480 490 500
LADDFLDFYL EYFPELKKKR VDIIPGFEFD RWLNTPGWPP YLPDLSPGDS
510 520 530 540 550
LMKPAEELAQ LWAAEELDMK AIEAVAISPW KTYQLVYFLD KILQKSPLPP
560 570 580 590 600
GNVKKLGDTY PSISNARNAE LRLRWGQIVL KNDHQEDFWK VKEFLHNQGK
610 620 630 640 650
QKYTLPLYHA MMGGSEVAQT LAKETFASTA SQLHSNVVNY VQQIVAPKGS
Length:650
Mass (Da):72,596
Last modified:March 27, 2002 - v2
Checksum:i4C04FE09689F2487
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti2A → V in CAC14047 (Ref. 2) Curated1
Sequence conflicti11 – 14GAAR → ARPGGRCTPRRL in CAC12957 (Ref. 1) Curated4
Sequence conflicti149G → R in CAC12957 (Ref. 1) Curated1
Sequence conflicti153L → V in CAC12957 (Ref. 1) Curated1
Sequence conflicti208 – 210STW → RPG in CAC12957 (Ref. 1) Curated3
Sequence conflicti262Missing in CAC12957 (Ref. 1) Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_051566579V → I. Corresponds to variant dbSNP:rs3820439Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ242586 mRNA. Translation: CAC12957.1.
AJ296161 mRNA. Translation: CAC14047.1.
BC001064 mRNA. Translation: AAH01064.1.
BC012166 mRNA. Translation: AAH12166.1.
AL390139 mRNA. Translation: CAB99087.1.
CCDSiCCDS1418.1.
PIRiT51870.
RefSeqiNP_001306111.1. NM_001319182.1.
NP_001306112.1. NM_001319183.1.
NP_001306113.1. NM_001319184.1.
NP_064601.3. NM_020216.3.
XP_005245478.1. XM_005245421.1.
XP_016857511.1. XM_017002022.1.
UniGeneiHs.497391.

Genome annotation databases

EnsembliENST00000295640; ENSP00000295640; ENSG00000176393.
GeneIDi6051.
KEGGihsa:6051.
UCSCiuc001gxd.4. human.

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiAMPB_HUMAN
AccessioniPrimary (citable) accession number: Q9H4A4
Secondary accession number(s): Q9BVM9, Q9H1D4, Q9NPT7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 27, 2002
Last sequence update: March 27, 2002
Last modified: November 22, 2017
This is version 160 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. Peptidase families
    Classification of peptidase families and list of entries
  6. SIMILARITY comments
    Index of protein domains and families