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Protein

Microtubule-associated proteins 1A/1B light chain 3A

Gene

MAP1LC3A

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes) (PubMed:20713600, PubMed:24290141). Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation (PubMed:20713600).2 Publications

GO - Molecular functioni

  • microtubule binding Source: GO_Central
  • phosphatidylethanolamine binding Source: UniProtKB
  • phospholipid binding Source: UniProtKB
  • ubiquitin protein ligase binding Source: UniProtKB

GO - Biological processi

  • autophagosome assembly Source: UniProtKB
  • cellular response to nitrogen starvation Source: GO_Central
  • macroautophagy Source: Reactome
  • mitophagy Source: MGI
Complete GO annotation...

Keywords - Biological processi

Autophagy, Ubl conjugation pathway

Enzyme and pathway databases

BioCyciZFISH:ENSG00000101460-MONOMER.
ReactomeiR-HSA-1632852. Macroautophagy.
R-HSA-5205685. Pink/Parkin Mediated Mitophagy.
SIGNORiQ9H492.

Names & Taxonomyi

Protein namesi
Recommended name:
Microtubule-associated proteins 1A/1B light chain 3A
Alternative name(s):
Autophagy-related protein LC3 A
Autophagy-related ubiquitin-like modifier LC3 A
MAP1 light chain 3-like protein 1
MAP1A/MAP1B light chain 3 A
Short name:
MAP1A/MAP1B LC3 A
Microtubule-associated protein 1 light chain 3 alpha
Gene namesi
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 20

Organism-specific databases

HGNCiHGNC:6838. MAP1LC3A.

Subcellular locationi

GO - Cellular componenti

  • autolysosome Source: MGI
  • autophagosome Source: UniProtKB
  • autophagosome membrane Source: GO_Central
  • cytoplasmic vesicle Source: UniProtKB-KW
  • cytosol Source: UniProtKB
  • late endosome Source: Ensembl
  • microtubule Source: UniProtKB-KW
  • organelle membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoplasmic vesicle, Cytoskeleton, Membrane, Microtubule

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi49K → A: Increases interaction with ATG13 and strongly reduces autophagosome formation. 1 Publication1
Mutagenesisi51K → A: Decreases interaction with ATG13 and strongly reduces autophagosome formation. 1 Publication1
Mutagenesisi53L → A: Decreases interaction with ATG13. 1 Publication1
Mutagenesisi120G → A: No processing of precursor. 1 Publication1

Organism-specific databases

DisGeNETi84557.
OpenTargetsiENSG00000101460.
PharmGKBiPA30582.

Polymorphism and mutation databases

BioMutaiMAP1LC3A.
DMDMi85701362.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000171921 – 120Microtubule-associated proteins 1A/1B light chain 3AAdd BLAST120
PropeptideiPRO_0000017193121Removed in mature form1

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei12Phosphoserine; by PKA1 Publication1
Lipidationi120Phosphatidylethanolamine amidated glycine1 Publication1

Post-translational modificationi

The precursor molecule is cleaved by ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II (PubMed:15187094).1 Publication
The Legionella effector RavZ is a deconjugating enzyme that produces an ATG8 product that would be resistant to reconjugation by the host machinery due to the cleavage of the reactive C-terminal glycine.1 Publication
Phosphorylation at Ser-12 by PKA inhibits conjugation to phosphatidylethanolamine (PE). Interaction with MAPK15 reduces the inhibitory phosphorylation and increases autophagy activity.2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei120 – 121Cleavage; by ATG4BBy similarity2

Keywords - PTMi

Lipoprotein, Phosphoprotein

Proteomic databases

EPDiQ9H492.
MaxQBiQ9H492.
PaxDbiQ9H492.
PeptideAtlasiQ9H492.
PRIDEiQ9H492.

PTM databases

iPTMnetiQ9H492.
PhosphoSitePlusiQ9H492.

Miscellaneous databases

PMAP-CutDBQ9H492.

Expressioni

Tissue specificityi

Most abundant in heart, brain, liver, skeletal muscle and testis but absent in thymus and peripheral blood leukocytes.1 Publication

Gene expression databases

BgeeiENSG00000101460.
CleanExiHS_MAP1LC3A.
GenevisibleiQ9H492. HS.

Organism-specific databases

HPAiCAB037174.
HPA007649.
HPA052474.
HPA052484.

Interactioni

Subunit structurei

3 different light chains, LC1, LC2 and LC3, can associate with MAP1A and MAP1B proteins (By similarity). Interacts with TP53INP1 and TP53INP2 (PubMed:19056683, PubMed:22470510). Directly interacts with SQSTM1; this interaction leads to MAP1LC3A recruitment to inclusion bodies containing polyubiquitinated protein aggregates and to inclusion body degradation by autophagy (PubMed:17580304). Interacts with ATG13 (PubMed:24290141, PubMed:23043107). Interacts with ULK1 (PubMed:23043107). Interacts with TBC1D5 (PubMed:22354992). Found in a complex with UBQLN1 and UBQLN2 (PubMed:20529957). Interacts with UBQLN4 (via STI1 1 and 2 domains). Interacts with UBQLN1 in the presence of UBQLN4 (PubMed:23459205). Interacts with TRIM5 (PubMed:25127057). Interacts with MEFV (PubMed:26347139). Interacts with FAM134A, FAM134B and FAM134C (PubMed:26040720).By similarity13 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
ATG4BQ9Y4P15EBI-720768,EBI-712014
Bnip3lQ9Z2F77EBI-720768,EBI-1774669From a different organism.
FAM134CQ86VR25EBI-720768,EBI-10192441
FUNDC1Q8IVP54EBI-720768,EBI-3059266
KXD1Q9BQD33EBI-720768,EBI-739657
NBR1Q145965EBI-720768,EBI-742698
SQSTM1Q135019EBI-720768,EBI-307104
TNIP1Q150255EBI-720768,EBI-357849
TOLLIPQ9H0E23EBI-720768,EBI-74615
UBQLN4Q9NRR53EBI-720768,EBI-711226

GO - Molecular functioni

  • microtubule binding Source: GO_Central
  • ubiquitin protein ligase binding Source: UniProtKB

Protein-protein interaction databases

BioGridi124137. 116 interactors.
DIPiDIP-49052N.
IntActiQ9H492. 386 interactors.
MINTiMINT-1420598.
STRINGi9606.ENSP00000363970.

Structurei

Secondary structure

1121
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi2 – 4Combined sources3
Helixi7 – 10Combined sources4
Helixi13 – 26Combined sources14
Beta strandi30 – 37Combined sources8
Beta strandi42 – 44Combined sources3
Beta strandi51 – 55Combined sources5
Helixi60 – 70Combined sources11
Beta strandi80 – 83Combined sources4
Turni84 – 86Combined sources3
Beta strandi91 – 93Combined sources3
Helixi95 – 102Combined sources8
Beta strandi109 – 116Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3ECIX-ray2.65A/B1-121[»]
3WALX-ray2.00A2-121[»]
3WANX-ray1.77A/B2-121[»]
4ZDVX-ray1.80A2-120[»]
5CX3X-ray2.30A/B/C/D1-121[»]
5DPRX-ray2.50A/B/C/D2-121[»]
ProteinModelPortaliQ9H492.
SMRiQ9H492.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9H492.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni49 – 53Important for interaction with ATG13 and for autophagosome formation1 Publication5

Sequence similaritiesi

Belongs to the ATG8 family.Curated

Phylogenomic databases

eggNOGiKOG1654. Eukaryota.
ENOG4111JAT. LUCA.
GeneTreeiENSGT00390000012937.
HOGENOMiHOG000232034.
HOVERGENiHBG051706.
InParanoidiQ9H492.
KOiK10435.
OMAiRTFADRC.
OrthoDBiEOG091G18ST.
PhylomeDBiQ9H492.
TreeFamiTF312964.

Family and domain databases

CDDicd01611. GABARAP. 1 hit.
InterProiIPR004241. Atg8-like.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PANTHERiPTHR10969. PTHR10969. 1 hit.
PfamiPF02991. Atg8. 1 hit.
[Graphical view]
SUPFAMiSSF54236. SSF54236. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9H492-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPSDRPFKQR RSFADRCKEV QQIRDQHPSK IPVIIERYKG EKQLPVLDKT
60 70 80 90 100
KFLVPDHVNM SELVKIIRRR LQLNPTQAFF LLVNQHSMVS VSTPIADIYE
110 120
QEKDEDGFLY MVYASQETFG F
Length:121
Mass (Da):14,272
Last modified:May 10, 2005 - v2
Checksum:i48C1FBE8F7892AF3
GO
Isoform 2 (identifier: Q9H492-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-13: MPSDRPFKQRRSF → MKMRFFSSPCGKAAVDP

Note: No experimental confirmation available.
Show »
Length:125
Mass (Da):14,493
Checksum:i097697B5424FC425
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0136601 – 13MPSDR…QRRSF → MKMRFFSSPCGKAAVDP in isoform 2. CuratedAdd BLAST13

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF276658 mRNA. Translation: AAK35151.1.
BT007452 mRNA. Translation: AAP36120.1.
AL833855 mRNA. Translation: CAD38714.1.
AL118520 Genomic DNA. Translation: CAC14078.1.
AL118520 Genomic DNA. Translation: CAI40290.1.
CH471077 Genomic DNA. Translation: EAW76263.1.
CH471077 Genomic DNA. Translation: EAW76264.1.
CH471077 Genomic DNA. Translation: EAW76265.1.
CH471077 Genomic DNA. Translation: EAW76266.1.
BC015810 mRNA. Translation: AAH15810.1.
CCDSiCCDS13237.1. [Q9H492-2]
CCDS13238.1. [Q9H492-1]
RefSeqiNP_115903.1. NM_032514.3. [Q9H492-1]
NP_852610.1. NM_181509.2. [Q9H492-2]
UniGeneiHs.632273.

Genome annotation databases

EnsembliENST00000360668; ENSP00000353886; ENSG00000101460. [Q9H492-1]
ENST00000374837; ENSP00000363970; ENSG00000101460. [Q9H492-2]
ENST00000397709; ENSP00000380821; ENSG00000101460. [Q9H492-1]
GeneIDi84557.
KEGGihsa:84557.
UCSCiuc002xap.3. human. [Q9H492-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF276658 mRNA. Translation: AAK35151.1.
BT007452 mRNA. Translation: AAP36120.1.
AL833855 mRNA. Translation: CAD38714.1.
AL118520 Genomic DNA. Translation: CAC14078.1.
AL118520 Genomic DNA. Translation: CAI40290.1.
CH471077 Genomic DNA. Translation: EAW76263.1.
CH471077 Genomic DNA. Translation: EAW76264.1.
CH471077 Genomic DNA. Translation: EAW76265.1.
CH471077 Genomic DNA. Translation: EAW76266.1.
BC015810 mRNA. Translation: AAH15810.1.
CCDSiCCDS13237.1. [Q9H492-2]
CCDS13238.1. [Q9H492-1]
RefSeqiNP_115903.1. NM_032514.3. [Q9H492-1]
NP_852610.1. NM_181509.2. [Q9H492-2]
UniGeneiHs.632273.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3ECIX-ray2.65A/B1-121[»]
3WALX-ray2.00A2-121[»]
3WANX-ray1.77A/B2-121[»]
4ZDVX-ray1.80A2-120[»]
5CX3X-ray2.30A/B/C/D1-121[»]
5DPRX-ray2.50A/B/C/D2-121[»]
ProteinModelPortaliQ9H492.
SMRiQ9H492.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi124137. 116 interactors.
DIPiDIP-49052N.
IntActiQ9H492. 386 interactors.
MINTiMINT-1420598.
STRINGi9606.ENSP00000363970.

PTM databases

iPTMnetiQ9H492.
PhosphoSitePlusiQ9H492.

Polymorphism and mutation databases

BioMutaiMAP1LC3A.
DMDMi85701362.

Proteomic databases

EPDiQ9H492.
MaxQBiQ9H492.
PaxDbiQ9H492.
PeptideAtlasiQ9H492.
PRIDEiQ9H492.

Protocols and materials databases

DNASUi84557.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000360668; ENSP00000353886; ENSG00000101460. [Q9H492-1]
ENST00000374837; ENSP00000363970; ENSG00000101460. [Q9H492-2]
ENST00000397709; ENSP00000380821; ENSG00000101460. [Q9H492-1]
GeneIDi84557.
KEGGihsa:84557.
UCSCiuc002xap.3. human. [Q9H492-1]

Organism-specific databases

CTDi84557.
DisGeNETi84557.
GeneCardsiMAP1LC3A.
HGNCiHGNC:6838. MAP1LC3A.
HPAiCAB037174.
HPA007649.
HPA052474.
HPA052484.
MIMi601242. gene.
neXtProtiNX_Q9H492.
OpenTargetsiENSG00000101460.
PharmGKBiPA30582.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1654. Eukaryota.
ENOG4111JAT. LUCA.
GeneTreeiENSGT00390000012937.
HOGENOMiHOG000232034.
HOVERGENiHBG051706.
InParanoidiQ9H492.
KOiK10435.
OMAiRTFADRC.
OrthoDBiEOG091G18ST.
PhylomeDBiQ9H492.
TreeFamiTF312964.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000101460-MONOMER.
ReactomeiR-HSA-1632852. Macroautophagy.
R-HSA-5205685. Pink/Parkin Mediated Mitophagy.
SIGNORiQ9H492.

Miscellaneous databases

ChiTaRSiMAP1LC3A. human.
EvolutionaryTraceiQ9H492.
GeneWikiiMAP1LC3A.
GenomeRNAii84557.
PMAP-CutDBQ9H492.
PROiQ9H492.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000101460.
CleanExiHS_MAP1LC3A.
GenevisibleiQ9H492. HS.

Family and domain databases

CDDicd01611. GABARAP. 1 hit.
InterProiIPR004241. Atg8-like.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PANTHERiPTHR10969. PTHR10969. 1 hit.
PfamiPF02991. Atg8. 1 hit.
[Graphical view]
SUPFAMiSSF54236. SSF54236. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMLP3A_HUMAN
AccessioniPrimary (citable) accession number: Q9H492
Secondary accession number(s): E1P5P4, E1P5P5, Q9BXW5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 8, 2002
Last sequence update: May 10, 2005
Last modified: November 30, 2016
This is version 140 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 20
    Human chromosome 20: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.