Q9H492 (MLP3A_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Microtubule-associated proteins 1A/1B light chain 3A

Alternative name(s):
Autophagy-related protein LC3 A
Autophagy-related ubiquitin-like modifier LC3 A
MAP1 light chain 3-like protein 1
MAP1A/MAP1B light chain 3 A
Short name=MAP1A/MAP1B LC3 A
Microtubule-associated protein 1 light chain 3 alpha

Gene names

Name:

MAP1LC3A

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	121 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in formation of autophagosomal vacuoles (autophagosomes).
Subunit structure	3 different light chains, LC1, LC2 and LC3, can associate with MAP1A and MAP1B proteins By similarity. Interacts with SQSTM1 By similarity. Interacts with TP53INP1 and TP53INP2. Directly interacts with SQSTM1; this interaction leads to MAP1LC3A recruitment to inclusion bodies containing polyubiquitinated protein aggregates and to inclusion body degradation by autophagy. Ref.8 Ref.9 Ref.11
Subcellular location	Cytoplasm › cytoskeleton. Endomembrane system; Lipid-anchor. Cytoplasmic vesicle › autophagosome membrane; Lipid-anchor. Cytoplasmic vesicle › autophagosome. Note: LC3-II binds to the autophagic membranes. Ref.1 Ref.8 Ref.9 Ref.10
Tissue specificity	Most abundant in heart, brain, liver, skeletal muscle and testis but absent in thymus and peripheral blood leukocytes. Ref.1
Post-translational modification	The precursor molecule is cleaved by APG4B/ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II. Ref.7
Sequence similarities	Belongs to the MAP1 LC3 family.

Ontologies

Keywords
Biological process	Autophagy Ubl conjugation pathway
Cellular component	Cytoplasm Cytoplasmic vesicle Cytoskeleton Membrane Microtubule
Coding sequence diversity	Alternative splicing
PTM	Lipoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	autophagic vacuole assembly Inferred from sequence or structural similarity. Source: UniProtKB mitochondrion degradation Inferred from genetic interaction PubMed 22267086. Source: MGI
Cellular_component	autophagic vacuole Inferred from direct assay Ref.1Ref.7Ref.9Ref.10. Source: UniProtKB autophagic vacuole membrane Inferred from electronic annotation. Source: UniProtKB-SubCell cytoplasmic vesicle Inferred from electronic annotation. Source: UniProtKB-KW cytosol Inferred from direct assay Ref.7. Source: UniProtKB endomembrane system Inferred from electronic annotation. Source: UniProtKB-SubCell late endosome Inferred from electronic annotation. Source: Compara microtubule Inferred from electronic annotation. Source: UniProtKB-KW organelle membrane Inferred from direct assay Ref.1. Source: UniProtKB
Molecular_function	phosphatidylethanolamine binding Inferred from direct assay PubMed 16303767. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
NBR1	Q14596	2	EBI-720768,EBI-742698
SQSTM1	Q13501	5	EBI-720768,EBI-307104

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9H492-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9H492-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-13: MPSDRPFKQRRSF → MKMRFFSSPCGKAAVDP

Note: No experimental confirmation available.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 120

120

Microtubule-associated proteins 1A/1B light chain 3A

PRO_0000017192

Propeptide

121

1

Removed in mature form

PRO_0000017193

Amino acid modifications

Lipidation

120

1

Phosphatidylethanolamine amidated glycine Probable

Natural variations

Alternative sequence

1 – 13

13

MPSDR…QRRSF → MKMRFFSSPCGKAAVDP in isoform 2.

VSP_013660

Experimental info

Mutagenesis

120

1

G → A: No processing of precursor. Ref.1

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

121

Helix Strand Turn

Details...

Helix

7 – 10

4

Helix

13 – 26

14

Beta strand

30 – 37

8

Beta strand

51 – 55

5

Helix

60 – 71

12

Beta strand

79 – 83

5

Turn

84 – 86

3

Helix

95 – 102

8

Beta strand

109 – 115

7

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified May 10, 2005. Version 2. Checksum: 48C1FBE8F7892AF3	FASTA	121	14,272
	10 20 30 40 50 60 MPSDRPFKQR RSFADRCKEV QQIRDQHPSK IPVIIERYKG EKQLPVLDKT KFLVPDHVNM 70 80 90 100 110 120 SELVKIIRRR LQLNPTQAFF LLVNQHSMVS VSTPIADIYE QEKDEDGFLY MVYASQETFG F
	Isoform 2 [UniParc]. Checksum: 097697B5424FC425	FASTA	125	14,493
	10 20 30 40 50 60 MKMRFFSSPC GKAAVDPADR CKEVQQIRDQ HPSKIPVIIE RYKGEKQLPV LDKTKFLVPD 70 80 90 100 110 120 HVNMSELVKI IRRRLQLNPT QAFFLLVNQH SMVSVSTPIA DIYEQEKDED GFLYMVYASQ ETFGF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Post-translational modifications of three members of the human MAP1LC3 family and detection of a novel type of modification for MAP1LC3B." He H., Dang Y., Dai F., Guo Z., Wu J., She X., Pei Y., Chen Y., Ling W., Wu C., Zhao S., Liu J.O., Yu L. J. Biol. Chem. 278:29278-29287(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), SUBCELLULAR LOCATION, TISSUE SPECIFICITY, MUTAGENESIS OF GLY-120.
[2]	"Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[3]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Amygdala.
[4]	"The DNA sequence and comparative analysis of human chromosome 20." Deloukas P., Matthews L.H., Ashurst J.L., Burton J., Gilbert J.G.R., Jones M., Stavrides G., Almeida J.P., Babbage A.K., Bagguley C.L., Bailey J., Barlow K.F., Bates K.N., Beard L.M., Beare D.M., Beasley O.P., Bird C.P., Blakey S.E. , Bridgeman A.M., Brown A.J., Buck D., Burrill W.D., Butler A.P., Carder C., Carter N.P., Chapman J.C., Clamp M., Clark G., Clark L.N., Clark S.Y., Clee C.M., Clegg S., Cobley V.E., Collier R.E., Connor R.E., Corby N.R., Coulson A., Coville G.J., Deadman R., Dhami P.D., Dunn M., Ellington A.G., Frankland J.A., Fraser A., French L., Garner P., Grafham D.V., Griffiths C., Griffiths M.N.D., Gwilliam R., Hall R.E., Hammond S., Harley J.L., Heath P.D., Ho S., Holden J.L., Howden P.J., Huckle E., Hunt A.R., Hunt S.E., Jekosch K., Johnson C.M., Johnson D., Kay M.P., Kimberley A.M., King A., Knights A., Laird G.K., Lawlor S., Lehvaeslaiho M.H., Leversha M.A., Lloyd C., Lloyd D.M., Lovell J.D., Marsh V.L., Martin S.L., McConnachie L.J., McLay K., McMurray A.A., Milne S.A., Mistry D., Moore M.J.F., Mullikin J.C., Nickerson T., Oliver K., Parker A., Patel R., Pearce T.A.V., Peck A.I., Phillimore B.J.C.T., Prathalingam S.R., Plumb R.W., Ramsay H., Rice C.M., Ross M.T., Scott C.E., Sehra H.K., Shownkeen R., Sims S., Skuce C.D., Smith M.L., Soderlund C., Steward C.A., Sulston J.E., Swann R.M., Sycamore N., Taylor R., Tee L., Thomas D.W., Thorpe A., Tracey A., Tromans A.C., Vaudin M., Wall M., Wallis J.M., Whitehead S.L., Whittaker P., Willey D.L., Williams L., Williams S.A., Wilming L., Wray P.W., Hubbard T., Durbin R.M., Bentley D.R., Beck S., Rogers J. Nature 414:865-871(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] (ISOFORMS 1 AND 2).
[5]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Uterus.
[7]	"HsAtg4B/HsApg4B/autophagin-1 cleaves the carboxyl termini of three human Atg8 homologues and delipidates microtubule-associated protein light chain 3- and GABAA receptor-associated protein-phospholipid conjugates." Tanida I., Sou Y.-S., Ezaki J., Minematsu-Ikeguchi N., Ueno T., Kominami E. J. Biol. Chem. 279:36268-36276(2004) [PubMed] [Europe PMC] [Abstract] Cited for: LIPIDATION, CLEAVAGE BY APG4B.
[8]	"p62/SQSTM1 binds directly to Atg8/LC3 to facilitate degradation of ubiquitinated protein aggregates by autophagy." Pankiv S., Clausen T.H., Lamark T., Brech A., Bruun J.A., Outzen H., Overvatn A., Bjorkoy G., Johansen T. J. Biol. Chem. 282:24131-24145(2007) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH SQSTM1, SUBCELLULAR LOCATION.
[9]	"The TP53INP2 protein is required for autophagy in mammalian cells." Nowak J., Archange C., Tardivel-Lacombe J., Pontarotti P., Pebusque M.J., Vaccaro M.I., Velasco G., Dagorn J.C., Iovanna J.L. Mol. Biol. Cell 20:870-881(2009) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION, INTERACTION WITH TP53INP2.
[10]	"TP53INP1, a tumor suppressor, interacts with LC3 and ATG8-family proteins through the LC3-interacting region (LIR) and promotes autophagy-dependent cell death." Seillier M., Peuget S., Gayet O., Gauthier C., N'guessan P., Monte M., Carrier A., Iovanna J.L., Dusetti N.J. Cell Death Differ. 19:1525-1535(2012) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION.
[11]	"DOR/Tp53inp2 and Tp53inp1 constitute a metazoan gene family encoding dual regulators of autophagy and transcription." Sancho A., Duran J., Garcia-Espana A., Mauvezin C., Alemu E.A., Lamark T., Macias M.J., Desalle R., Royo M., Sala D., Chicote J.U., Palacin M., Johansen T., Zorzano A. PLoS ONE 7:E34034-E34034(2012) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH TP53INP1 AND TP53INP2.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF276658 mRNA. Translation: AAK35151.1.
BT007452 mRNA. Translation: AAP36120.1.
AL833855 mRNA. Translation: CAD38714.1.
AL118520 Genomic DNA. Translation: CAC14078.1.
AL118520 Genomic DNA. Translation: CAI40290.1.
CH471077 Genomic DNA. Translation: EAW76263.1.
CH471077 Genomic DNA. Translation: EAW76264.1.
CH471077 Genomic DNA. Translation: EAW76265.1.
CH471077 Genomic DNA. Translation: EAW76266.1.
BC015810 mRNA. Translation: AAH15810.1.

IPI

IPI00015423.
IPI00415014.

RefSeq

NP_115903.1. NM_032514.3.
NP_852610.1. NM_181509.2.

UniGene

Hs.632273.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3ECI	X-ray	2.65	A/B	1-121	[»]

ProteinModelPortal

Q9H492.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q9H492. 375 interactions.

MINT

MINT-1420598.

STRING

9606.ENSP00000363970.

PTM databases

PhosphoSite

Q9H492.

Polymorphism databases

DMDM

85701362.

Proteomic databases

PaxDb

Q9H492.

PRIDE

Q9H492.

Protocols and materials databases

DNASU

84557.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000360668; ENSP00000353886; ENSG00000101460.
ENST00000374837; ENSP00000363970; ENSG00000101460.
ENST00000397709; ENSP00000380821; ENSG00000101460.

GeneID

84557.

KEGG

hsa:84557.

UCSC

uc002xap.1. human.
uc002xaq.1. human.

Organism-specific databases

CTD

84557.

GeneCards

GC20P033134.

HGNC

HGNC:6838. MAP1LC3A.

HPA

HPA007649.

MIM

601242. gene.

neXtProt

NX_Q9H492.

PharmGKB

PA30582.

GenAtlas

Search...

Phylogenomic databases

eggNOG

NOG264390.

HOGENOM

HOG000232034.

HOVERGEN

HBG051706.

KO

K10435.

OMA

TPISEIY.

OrthoDB

EOG4PC9TJ.

Gene expression databases

Bgee

Q9H492.

CleanEx

HS_MAP1LC3A.

Genevestigator

Q9H492.

GermOnline

ENSG00000101460. Homo sapiens.

Family and domain databases

InterPro

IPR004241. Atg8_ubiquitin-like.
[Graphical view]

PANTHER

PTHR10969. PTHR10969. 1 hit.

Pfam

PF02991. Atg8. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

Q9H492.

GenomeRNAi

84557.

NextBio

74441.

PMAP-CutDB

Q9H492.

SOURCE

Search...

Entry information

Entry name

MLP3A_HUMAN

Accession

Primary (citable) accession number: Q9H492
Secondary accession number(s): E1P5P4, E1P5P5, Q9BXW5

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 8, 2002
Last sequence update:	May 10, 2005
Last modified:	May 1, 2013
This is version 102 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.