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Protein

Microtubule-associated proteins 1A/1B light chain 3A

Gene

MAP1LC3A

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes) (PubMed:20713600, PubMed:24290141). Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation (PubMed:20713600).2 Publications

Caution

GO - Molecular functioni

  • phosphatidylethanolamine binding Source: UniProtKB
  • phospholipid binding Source: UniProtKB
  • ubiquitin protein ligase binding Source: UniProtKB

GO - Biological processi

  • autophagosome assembly Source: UniProtKB
  • autophagosome maturation Source: UniProtKB
  • autophagy of mitochondrion Source: MGI
  • cellular response to amino acid starvation Source: Ensembl
  • cellular response to copper ion Source: Ensembl
  • cellular response to hydrogen peroxide Source: Ensembl
  • cellular response to nitrogen starvation Source: GO_Central
  • cellular response to starvation Source: UniProtKB
  • macroautophagy Source: UniProtKB
  • response to iron(II) ion Source: Ensembl
  • response to lead ion Source: Ensembl
  • response to morphine Source: Ensembl

Keywordsi

Biological processAutophagy, Ubl conjugation pathway

Enzyme and pathway databases

ReactomeiR-HSA-1632852 Macroautophagy
R-HSA-5205685 Pink/Parkin Mediated Mitophagy
R-HSA-8934903 Receptor Mediated Mitophagy
SIGNORiQ9H492

Names & Taxonomyi

Protein namesi
Recommended name:
Microtubule-associated proteins 1A/1B light chain 3A
Alternative name(s):
Autophagy-related protein LC3 A
Autophagy-related ubiquitin-like modifier LC3 A
MAP1 light chain 3-like protein 1
MAP1A/MAP1B light chain 3 A
Short name:
MAP1A/MAP1B LC3 A
Microtubule-associated protein 1 light chain 3 alpha
Gene namesi
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 20

Organism-specific databases

EuPathDBiHostDB:ENSG00000101460.12
HGNCiHGNC:6838 MAP1LC3A
MIMi601242 gene
neXtProtiNX_Q9H492

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoplasmic vesicle, Cytoskeleton, Membrane, Microtubule

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi49K → A: Increases interaction with ATG13 and strongly reduces autophagosome formation. 1 Publication1
Mutagenesisi51K → A: Decreases interaction with ATG13 and strongly reduces autophagosome formation. 1 Publication1
Mutagenesisi53L → A: Decreases interaction with ATG13. 1 Publication1
Mutagenesisi120G → A: No processing of precursor. 1 Publication1

Organism-specific databases

DisGeNETi84557
OpenTargetsiENSG00000101460
PharmGKBiPA30582

Polymorphism and mutation databases

BioMutaiMAP1LC3A
DMDMi85701362

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000171921 – 120Microtubule-associated proteins 1A/1B light chain 3AAdd BLAST120
PropeptideiPRO_0000017193121Removed in mature form1

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei12Phosphoserine; by PKA1 Publication1
Lipidationi120Phosphatidylethanolamine amidated glycine1 Publication1

Post-translational modificationi

The precursor molecule is cleaved by ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II (PubMed:15187094).1 Publication
The Legionella effector RavZ is a deconjugating enzyme that produces an ATG8 product that would be resistant to reconjugation by the host machinery due to the cleavage of the reactive C-terminal glycine.1 Publication
Phosphorylation at Ser-12 by PKA inhibits conjugation to phosphatidylethanolamine (PE). Interaction with MAPK15 reduces the inhibitory phosphorylation and increases autophagy activity.2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei120 – 121Cleavage; by ATG4BBy similarity2

Keywords - PTMi

Lipoprotein, Phosphoprotein

Proteomic databases

EPDiQ9H492
MaxQBiQ9H492
PaxDbiQ9H492
PeptideAtlasiQ9H492
PRIDEiQ9H492

PTM databases

iPTMnetiQ9H492
PhosphoSitePlusiQ9H492

Miscellaneous databases

PMAP-CutDBiQ9H492

Expressioni

Tissue specificityi

Most abundant in heart, brain, liver, skeletal muscle and testis but absent in thymus and peripheral blood leukocytes.1 Publication

Gene expression databases

BgeeiENSG00000101460
CleanExiHS_MAP1LC3A
GenevisibleiQ9H492 HS

Organism-specific databases

HPAiCAB037174
HPA007649
HPA043000
HPA052474
HPA052484

Interactioni

Subunit structurei

3 different light chains, LC1, LC2 and LC3, can associate with MAP1A and MAP1B proteins (By similarity). Interacts with TP53INP1 and TP53INP2 (PubMed:19056683, PubMed:22470510). Directly interacts with SQSTM1; this interaction leads to MAP1LC3A recruitment to inclusion bodies containing polyubiquitinated protein aggregates and to inclusion body degradation by autophagy (PubMed:17580304). Interacts with ATG13 (PubMed:24290141, PubMed:23043107). Interacts with ULK1 (PubMed:23043107). Interacts with TBC1D5 (PubMed:22354992). Found in a complex with UBQLN1 and UBQLN2 (PubMed:20529957). Interacts with UBQLN4 (via STI1 1 and 2 domains). Interacts with UBQLN1 in the presence of UBQLN4 (PubMed:23459205). Interacts with TRIM5 (PubMed:25127057). Interacts with MEFV (PubMed:26347139). Interacts with RETREG1, RETREG2 and RETREG3 (PubMed:26040720).By similarity13 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • ubiquitin protein ligase binding Source: UniProtKB

Protein-protein interaction databases

BioGridi124137120 interactors.
CORUMiQ9H492
DIPiDIP-49052N
ELMiQ9H492
IntActiQ9H492 394 interactors.
MINTiQ9H492
STRINGi9606.ENSP00000363970

Structurei

Secondary structure

1121
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi2 – 4Combined sources3
Helixi7 – 10Combined sources4
Helixi13 – 26Combined sources14
Beta strandi30 – 37Combined sources8
Beta strandi42 – 44Combined sources3
Beta strandi51 – 55Combined sources5
Helixi60 – 70Combined sources11
Beta strandi80 – 83Combined sources4
Turni84 – 86Combined sources3
Beta strandi91 – 93Combined sources3
Helixi95 – 102Combined sources8
Beta strandi109 – 116Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3ECIX-ray2.65A/B1-121[»]
3WALX-ray2.00A2-121[»]
3WANX-ray1.77A/B2-121[»]
4ZDVX-ray1.80A2-120[»]
5CX3X-ray2.30A/B/C/D1-121[»]
5DPRX-ray2.50A/B/C/D2-121[»]
ProteinModelPortaliQ9H492
SMRiQ9H492
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9H492

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni49 – 53Important for interaction with ATG13 and for autophagosome formation1 Publication5

Sequence similaritiesi

Belongs to the ATG8 family.Curated

Phylogenomic databases

eggNOGiKOG1654 Eukaryota
ENOG4111JAT LUCA
GeneTreeiENSGT00390000012937
HOGENOMiHOG000232034
HOVERGENiHBG051706
InParanoidiQ9H492
KOiK10435
OMAiRTFADRC
OrthoDBiEOG091G18ST
PhylomeDBiQ9H492
TreeFamiTF312964

Family and domain databases

CDDicd01611 GABARAP, 1 hit
InterProiView protein in InterPro
IPR004241 Atg8-like
IPR029071 Ubiquitin-like_domsf
PANTHERiPTHR10969 PTHR10969, 1 hit
PfamiView protein in Pfam
PF02991 Atg8, 1 hit
SUPFAMiSSF54236 SSF54236, 1 hit

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9H492-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPSDRPFKQR RSFADRCKEV QQIRDQHPSK IPVIIERYKG EKQLPVLDKT
60 70 80 90 100
KFLVPDHVNM SELVKIIRRR LQLNPTQAFF LLVNQHSMVS VSTPIADIYE
110 120
QEKDEDGFLY MVYASQETFG F
Length:121
Mass (Da):14,272
Last modified:May 10, 2005 - v2
Checksum:i48C1FBE8F7892AF3
GO
Isoform 2 (identifier: Q9H492-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-13: MPSDRPFKQRRSF → MKMRFFSSPCGKAAVDP

Note: No experimental confirmation available.
Show »
Length:125
Mass (Da):14,493
Checksum:i097697B5424FC425
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0136601 – 13MPSDR…QRRSF → MKMRFFSSPCGKAAVDP in isoform 2. CuratedAdd BLAST13

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF276658 mRNA Translation: AAK35151.1
BT007452 mRNA Translation: AAP36120.1
AL833855 mRNA Translation: CAD38714.1
AL118520 Genomic DNA No translation available.
CH471077 Genomic DNA Translation: EAW76263.1
CH471077 Genomic DNA Translation: EAW76264.1
CH471077 Genomic DNA Translation: EAW76265.1
CH471077 Genomic DNA Translation: EAW76266.1
BC015810 mRNA Translation: AAH15810.1
CCDSiCCDS13237.1 [Q9H492-2]
CCDS13238.1 [Q9H492-1]
RefSeqiNP_115903.1, NM_032514.3 [Q9H492-1]
NP_852610.1, NM_181509.2 [Q9H492-2]
UniGeneiHs.632273

Genome annotation databases

EnsembliENST00000360668; ENSP00000353886; ENSG00000101460 [Q9H492-1]
ENST00000374837; ENSP00000363970; ENSG00000101460 [Q9H492-2]
ENST00000397709; ENSP00000380821; ENSG00000101460 [Q9H492-1]
GeneIDi84557
KEGGihsa:84557
UCSCiuc002xap.3 human [Q9H492-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiMLP3A_HUMAN
AccessioniPrimary (citable) accession number: Q9H492
Secondary accession number(s): E1P5P4, E1P5P5, Q9BXW5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 8, 2002
Last sequence update: May 10, 2005
Last modified: April 25, 2018
This is version 153 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome