Reviewed, UniProtKB/Swiss-Prot Q9H490 (PIGU_HUMAN)

Names and origin

Protein names

Recommended name:
    Phosphatidylinositol glycan anchor biosynthesis class U protein
Alternative name(s):
    GPI transamidase component PIG-U
    Cell division cycle protein 91-like 1
      Short name=Protein CDC91-like 1

Gene names

Name:	PIGU
Synonyms:	CDC91L1
ORF Names:	PSEC0205, UNQ3055/PRO9875

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	435 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Component of the GPI transamidase complex. May be involved in the recognition of either the GPI attachment signal or the lipid portion of GPI. Ref.1
Pathway	Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.
Subunit structure	Forms a complex with PIGK/GPI8, PIGS, PIGT and GPAA1/GAA1. Ref.1
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein Potential.
Sequence similarities	Belongs to the PIGU family.

Ontologies

Keywords
Biological process	GPI-anchor biosynthesis
Cellular component	Endoplasmic reticulum Membrane
Coding sequence diversity	Alternative splicing
Domain	Transmembrane
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	attachment of GPI anchor to protein Traceable author statement. Source: UniProtKB regulation of JAK-STAT cascade Inferred from direct assay. Source: HGNC
Cellular component	GPI-anchor transamidase complex Ref.1 Inferred from mutant phenotype. Source: UniProtKB plasma membrane Inferred from direct assay. Source: HGNC
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9H490-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9H490-2)

The sequence of this isoform differs from the canonical sequence as follows:
     66-85: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.1
	Chain	2 – 435	434	Phosphatidylinositol glycan anchor biosynthesis class U protein		PRO_0000121394
Regions
	Transmembrane	66 – 86	21	Potential
	Transmembrane	166 – 186	21	Potential
	Transmembrane	188 – 208	21	Potential
	Transmembrane	237 – 257	21	Potential
	Transmembrane	259 – 279	21	Potential
	Transmembrane	286 – 306	21	Potential
	Transmembrane	313 – 333	21	Potential
	Transmembrane	355 – 375	21	Potential
	Transmembrane	386 – 406	21	Potential
	Region	269 – 289	21	May be involved in recognition of long-chain fatty acids in GPI
Natural variations
	Alternative sequence	66 – 85	20	Missing in isoform 2.		VSP_009543
Experimental info
	Sequence conflict	407	1	Y → N in BAC11660. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 65FDD796AB533027	FASTA	435	50,052
	10 20 30 40 50 60 MAAPLVLVLV VAVTVRAALF RSSLAEFISE RVEVVSPLSS WKRVVEGLSL LDLGVSPYSG 70 80 90 100 110 120 AVFHETPLII YLFHFLIDYA ELVFMITDAL TAIALYFAIQ DFNKVVFKKQ KLLLELDQYA 130 140 150 160 170 180 PDVAELIRTP MEMRYIPLKV ALFYLLNPYT ILSCVAKSTC AINNTLIAFF ILTTIKGSAF 190 200 210 220 230 240 LSAIFLALAT YQSLYPLTLF VPGLLYLLQR QYIPVKMKSK AFWIFSWEYA MMYVGSLVVI 250 260 270 280 290 300 ICLSFFLLSS WDFIPAVYGF ILSVPDLTPN IGLFWYFFAE MFEHFSLFFV CVFQINVFFY 310 320 330 340 350 360 TIPLAIKLKE HPIFFMFIQI AVIAIFKSYP TVGDVALYMA FFPVWNHLYR FLRNIFVLTC 370 380 390 400 410 420 IIIVCSLLFP VLWHLWIYAG SANSNFFYAI TLTFNVGQIL LISDYFYAFL RREYYLTHGL 430 YLTAKDGTEA MLVLK
	Isoform 2. Checksum: 45BA0934D0273E71	FASTA	415	47,611
	10 20 30 40 50 60 MAAPLVLVLV VAVTVRAALF RSSLAEFISE RVEVVSPLSS WKRVVEGLSL LDLGVSPYSG 70 80 90 100 110 120 AVFHEITDAL TAIALYFAIQ DFNKVVFKKQ KLLLELDQYA PDVAELIRTP MEMRYIPLKV 130 140 150 160 170 180 ALFYLLNPYT ILSCVAKSTC AINNTLIAFF ILTTIKGSAF LSAIFLALAT YQSLYPLTLF 190 200 210 220 230 240 VPGLLYLLQR QYIPVKMKSK AFWIFSWEYA MMYVGSLVVI ICLSFFLLSS WDFIPAVYGF 250 260 270 280 290 300 ILSVPDLTPN IGLFWYFFAE MFEHFSLFFV CVFQINVFFY TIPLAIKLKE HPIFFMFIQI 310 320 330 340 350 360 AVIAIFKSYP TVGDVALYMA FFPVWNHLYR FLRNIFVLTC IIIVCSLLFP VLWHLWIYAG 370 380 390 400 410 SANSNFFYAI TLTFNVGQIL LISDYFYAFL RREYYLTHGL YLTAKDGTEA MLVLK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Human PIG-U and yeast Cdc91p are the fifth subunit of GPI transamidase that attaches GPI-anchors to proteins." Hong Y., Ohishi K., Kang J.Y., Tanaka S., Inoue N., Nishimura J., Maeda Y., Kinoshita T. Mol. Biol. Cell 14:1780-1789(2003) [PubMed: 12802054] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), PROTEIN SEQUENCE OF 2-8, FUNCTION, SUBUNIT. Tissue: Embryo.
[2]	"PIG-U is a novel oncogene in human bladder cancer." Guo Z., Wu G., Sidransky D., Trink B. Submitted (SEP-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[3]	"HRI human cDNA sequencing project." Ota T., Nishikawa T., Suzuki Y., Kawai-Hio Y., Hayashi K., Ishii S., Saito K., Yamamoto J., Wakamatsu A., Nagai T., Nakamura Y., Nagahari K., Sugano S., Isogai T. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Embryo.
[4]	Lin L., Yu R., Ke R., Li H., Zhou G., Shen C., Zheng G., Zhong G., Li M., Xiao W., Yang S. Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
[5]	"The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment." Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J., Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P., Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E. , Heldens S., Huang A., Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D., Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L., Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C., Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J., Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M. Genome Res. 13:2265-2270(2003) [PubMed: 12975309] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[6]	"The DNA sequence and comparative analysis of human chromosome 20." Deloukas P., Matthews L.H., Ashurst J.L., Burton J., Gilbert J.G.R., Jones M., Stavrides G., Almeida J.P., Babbage A.K., Bagguley C.L., Bailey J., Barlow K.F., Bates K.N., Beard L.M., Beare D.M., Beasley O.P., Bird C.P., Blakey S.E. , Bridgeman A.M., Brown A.J., Buck D., Burrill W.D., Butler A.P., Carder C., Carter N.P., Chapman J.C., Clamp M., Clark G., Clark L.N., Clark S.Y., Clee C.M., Clegg S., Cobley V.E., Collier R.E., Connor R.E., Corby N.R., Coulson A., Coville G.J., Deadman R., Dhami P.D., Dunn M., Ellington A.G., Frankland J.A., Fraser A., French L., Garner P., Grafham D.V., Griffiths C., Griffiths M.N.D., Gwilliam R., Hall R.E., Hammond S., Harley J.L., Heath P.D., Ho S., Holden J.L., Howden P.J., Huckle E., Hunt A.R., Hunt S.E., Jekosch K., Johnson C.M., Johnson D., Kay M.P., Kimberley A.M., King A., Knights A., Laird G.K., Lawlor S., Lehvaeslaiho M.H., Leversha M.A., Lloyd C., Lloyd D.M., Lovell J.D., Marsh V.L., Martin S.L., McConnachie L.J., McLay K., McMurray A.A., Milne S.A., Mistry D., Moore M.J.F., Mullikin J.C., Nickerson T., Oliver K., Parker A., Patel R., Pearce T.A.V., Peck A.I., Phillimore B.J.C.T., Prathalingam S.R., Plumb R.W., Ramsay H., Rice C.M., Ross M.T., Scott C.E., Sehra H.K., Shownkeen R., Sims S., Skuce C.D., Smith M.L., Soderlund C., Steward C.A., Sulston J.E., Swann R.M., Sycamore N., Taylor R., Tee L., Thomas D.W., Thorpe A., Tracey A., Tromans A.C., Vaudin M., Wall M., Wallis J.M., Whitehead S.L., Whittaker P., Willey D.L., Williams L., Williams S.A., Wilming L., Wray P.W., Hubbard T., Durbin R.M., Bentley D.R., Beck S., Rogers J. Nature 414:865-871(2001) [PubMed: 11780052] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB086842 mRNA. Translation: BAC53626.1. AY422169 mRNA. Translation: AAR23798.1. AK075507 mRNA. Translation: BAC11660.1. AY339061 mRNA. Translation: AAQ18022.1. AY358816 mRNA. Translation: AAQ89175.1. AL118520 Genomic DNA. Translation: CAC14080.1. BC030512 mRNA. Translation: AAH30512.1.
IPI	IPI00026044. IPI00402109.
RefSeq	NP_536724.1.
UniGene	Hs.253319
3D structure databases
ModBase	Search...
Proteomic databases
PRIDE	Q9H490.
Genome annotation databases
Ensembl	ENSG00000101464. Homo sapiens. [Contig view]
GeneID	128869.
KEGG	hsa:128869.
Organism-specific databases
GeneCards	GC20M032613.
H-InvDB	HIX0015749. HIX0040493.
HGNC	HGNC:15791. PIGU.
MIM	608528. gene.
PharmGKB	PA26273.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	Q9H490.
HOVERGEN	Q9H490.
OMA	Q9H490. EMFEHFR.
Enzyme and pathway databases
Reactome	REACT_1069. Post-translational protein modification.
Gene expression databases
ArrayExpress	Q9H490.
Bgee	Q9H490.
CleanEx	HS_PIGU.
GermOnline	ENSG00000101464. Homo sapiens.
Family and domain databases
InterPro	IPR009600. PIG-U. [Graphical view]
PANTHER	PTHR13121. PIG-U. 1 hit.
Pfam	PF06728. PIG-U. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	82506.
SOURCE	Search...

Entry information

Entry name

PIGU_HUMAN

Accession

Primary (citable) accession number: Q9H490
Secondary accession number(s): Q7Z489, Q8N2F2

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 25, 2003
Last sequence update:	January 23, 2007
Last modified:	June 16, 2009
This is version 63 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)