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Protein

Phosphatidylinositol glycan anchor biosynthesis class U protein

Gene

PIGU

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the GPI transamidase complex. May be involved in the recognition of either the GPI attachment signal or the lipid portion of GPI.1 Publication

Pathwayi: glycosylphosphatidylinositol-anchor biosynthesis

This protein is involved in the pathway glycosylphosphatidylinositol-anchor biosynthesis, which is part of Glycolipid biosynthesis.
View all proteins of this organism that are known to be involved in the pathway glycosylphosphatidylinositol-anchor biosynthesis and in Glycolipid biosynthesis.

GO - Biological processi

  • attachment of GPI anchor to protein Source: UniProtKB
  • cellular protein metabolic process Source: Reactome
  • C-terminal protein lipidation Source: Reactome
  • GPI anchor biosynthetic process Source: HGNC
  • post-translational protein modification Source: Reactome
  • regulation of JAK-STAT cascade Source: HGNC
Complete GO annotation...

Keywords - Biological processi

GPI-anchor biosynthesis

Enzyme and pathway databases

ReactomeiR-HSA-162791. Attachment of GPI anchor to uPAR.
UniPathwayiUPA00196.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphatidylinositol glycan anchor biosynthesis class U protein
Alternative name(s):
Cell division cycle protein 91-like 1
Short name:
Protein CDC91-like 1
GPI transamidase component PIG-U
Gene namesi
Name:PIGU
Synonyms:CDC91L1
ORF Names:PSEC0205, UNQ3055/PRO9875
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 20

Organism-specific databases

HGNCiHGNC:15791. PIGU.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei66 – 8621HelicalSequence analysisAdd
BLAST
Transmembranei166 – 18621HelicalSequence analysisAdd
BLAST
Transmembranei188 – 20821HelicalSequence analysisAdd
BLAST
Transmembranei237 – 25721HelicalSequence analysisAdd
BLAST
Transmembranei259 – 27921HelicalSequence analysisAdd
BLAST
Transmembranei286 – 30621HelicalSequence analysisAdd
BLAST
Transmembranei313 – 33321HelicalSequence analysisAdd
BLAST
Transmembranei355 – 37521HelicalSequence analysisAdd
BLAST
Transmembranei386 – 40621HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

  • endoplasmic reticulum membrane Source: Reactome
  • GPI-anchor transamidase complex Source: UniProtKB
  • integral component of endoplasmic reticulum membrane Source: UniProtKB
  • membrane Source: UniProtKB
  • plasma membrane Source: HGNC
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA162399506.

Polymorphism and mutation databases

BioMutaiPIGU.
DMDMi29336947.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemoved1 Publication
Chaini2 – 435434Phosphatidylinositol glycan anchor biosynthesis class U proteinPRO_0000121394Add
BLAST

Proteomic databases

EPDiQ9H490.
MaxQBiQ9H490.
PaxDbiQ9H490.
PRIDEiQ9H490.

PTM databases

iPTMnetiQ9H490.
PhosphoSiteiQ9H490.

Expressioni

Gene expression databases

BgeeiQ9H490.
CleanExiHS_PIGU.
ExpressionAtlasiQ9H490. baseline and differential.
GenevisibleiQ9H490. HS.

Organism-specific databases

HPAiHPA041706.
HPA046766.

Interactioni

Subunit structurei

Forms a complex with PIGK/GPI8, PIGS, PIGT and GPAA1/GAA1.1 Publication

Protein-protein interaction databases

BioGridi126171. 27 interactions.
IntActiQ9H490. 4 interactions.
STRINGi9606.ENSP00000217446.

Structurei

3D structure databases

ProteinModelPortaliQ9H490.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni269 – 28921May be involved in recognition of long-chain fatty acids in GPIAdd
BLAST

Sequence similaritiesi

Belongs to the PIGU family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2552. Eukaryota.
ENOG410XST9. LUCA.
GeneTreeiENSGT00390000014941.
HOGENOMiHOG000189223.
HOVERGENiHBG082139.
InParanoidiQ9H490.
KOiK05293.
OMAiGKEIRQI.
OrthoDBiEOG7CNZFR.
PhylomeDBiQ9H490.
TreeFamiTF101063.

Family and domain databases

InterProiIPR009600. PIG-U.
[Graphical view]
PANTHERiPTHR13121. PTHR13121. 1 hit.
PfamiPF06728. PIG-U. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9H490-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAAPLVLVLV VAVTVRAALF RSSLAEFISE RVEVVSPLSS WKRVVEGLSL
60 70 80 90 100
LDLGVSPYSG AVFHETPLII YLFHFLIDYA ELVFMITDAL TAIALYFAIQ
110 120 130 140 150
DFNKVVFKKQ KLLLELDQYA PDVAELIRTP MEMRYIPLKV ALFYLLNPYT
160 170 180 190 200
ILSCVAKSTC AINNTLIAFF ILTTIKGSAF LSAIFLALAT YQSLYPLTLF
210 220 230 240 250
VPGLLYLLQR QYIPVKMKSK AFWIFSWEYA MMYVGSLVVI ICLSFFLLSS
260 270 280 290 300
WDFIPAVYGF ILSVPDLTPN IGLFWYFFAE MFEHFSLFFV CVFQINVFFY
310 320 330 340 350
TIPLAIKLKE HPIFFMFIQI AVIAIFKSYP TVGDVALYMA FFPVWNHLYR
360 370 380 390 400
FLRNIFVLTC IIIVCSLLFP VLWHLWIYAG SANSNFFYAI TLTFNVGQIL
410 420 430
LISDYFYAFL RREYYLTHGL YLTAKDGTEA MLVLK
Length:435
Mass (Da):50,052
Last modified:January 23, 2007 - v3
Checksum:i65FDD796AB533027
GO
Isoform 2 (identifier: Q9H490-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     66-85: Missing.

Note: No experimental confirmation available.
Show »
Length:415
Mass (Da):47,611
Checksum:i45BA0934D0273E71
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti407 – 4071Y → N in BAC11660 (PubMed:16303743).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei66 – 8520Missing in isoform 2. 1 PublicationVSP_009543Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB086842 mRNA. Translation: BAC53626.1.
AY422169 mRNA. Translation: AAR23798.1.
AY339061 mRNA. Translation: AAQ18022.1.
AY358816 mRNA. Translation: AAQ89175.1.
AK075507 mRNA. Translation: BAC11660.1.
AL118520 Genomic DNA. Translation: CAC14080.1.
BC030512 mRNA. Translation: AAH30512.1.
CCDSiCCDS13239.1. [Q9H490-1]
RefSeqiNP_536724.1. NM_080476.4. [Q9H490-1]
UniGeneiHs.253319.

Genome annotation databases

EnsembliENST00000217446; ENSP00000217446; ENSG00000101464. [Q9H490-1]
ENST00000374820; ENSP00000363953; ENSG00000101464. [Q9H490-2]
GeneIDi128869.
KEGGihsa:128869.
UCSCiuc002xas.4. human. [Q9H490-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB086842 mRNA. Translation: BAC53626.1.
AY422169 mRNA. Translation: AAR23798.1.
AY339061 mRNA. Translation: AAQ18022.1.
AY358816 mRNA. Translation: AAQ89175.1.
AK075507 mRNA. Translation: BAC11660.1.
AL118520 Genomic DNA. Translation: CAC14080.1.
BC030512 mRNA. Translation: AAH30512.1.
CCDSiCCDS13239.1. [Q9H490-1]
RefSeqiNP_536724.1. NM_080476.4. [Q9H490-1]
UniGeneiHs.253319.

3D structure databases

ProteinModelPortaliQ9H490.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi126171. 27 interactions.
IntActiQ9H490. 4 interactions.
STRINGi9606.ENSP00000217446.

PTM databases

iPTMnetiQ9H490.
PhosphoSiteiQ9H490.

Polymorphism and mutation databases

BioMutaiPIGU.
DMDMi29336947.

Proteomic databases

EPDiQ9H490.
MaxQBiQ9H490.
PaxDbiQ9H490.
PRIDEiQ9H490.

Protocols and materials databases

DNASUi128869.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000217446; ENSP00000217446; ENSG00000101464. [Q9H490-1]
ENST00000374820; ENSP00000363953; ENSG00000101464. [Q9H490-2]
GeneIDi128869.
KEGGihsa:128869.
UCSCiuc002xas.4. human. [Q9H490-1]

Organism-specific databases

CTDi128869.
GeneCardsiPIGU.
HGNCiHGNC:15791. PIGU.
HPAiHPA041706.
HPA046766.
MIMi608528. gene.
neXtProtiNX_Q9H490.
PharmGKBiPA162399506.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2552. Eukaryota.
ENOG410XST9. LUCA.
GeneTreeiENSGT00390000014941.
HOGENOMiHOG000189223.
HOVERGENiHBG082139.
InParanoidiQ9H490.
KOiK05293.
OMAiGKEIRQI.
OrthoDBiEOG7CNZFR.
PhylomeDBiQ9H490.
TreeFamiTF101063.

Enzyme and pathway databases

UniPathwayiUPA00196.
ReactomeiR-HSA-162791. Attachment of GPI anchor to uPAR.

Miscellaneous databases

ChiTaRSiPIGU. human.
GeneWikiiPIGU.
GenomeRNAii128869.
NextBioi82506.
PROiQ9H490.
SOURCEiSearch...

Gene expression databases

BgeeiQ9H490.
CleanExiHS_PIGU.
ExpressionAtlasiQ9H490. baseline and differential.
GenevisibleiQ9H490. HS.

Family and domain databases

InterProiIPR009600. PIG-U.
[Graphical view]
PANTHERiPTHR13121. PTHR13121. 1 hit.
PfamiPF06728. PIG-U. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Human PIG-U and yeast Cdc91p are the fifth subunit of GPI transamidase that attaches GPI-anchors to proteins."
    Hong Y., Ohishi K., Kang J.Y., Tanaka S., Inoue N., Nishimura J., Maeda Y., Kinoshita T.
    Mol. Biol. Cell 14:1780-1789(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), PROTEIN SEQUENCE OF 2-8, FUNCTION, SUBUNIT.
    Tissue: Embryo.
  2. "PIG-U is a novel oncogene in human bladder cancer."
    Guo Z., Wu G., Sidransky D., Trink B.
    Submitted (SEP-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
  3. Lin L., Yu R., Ke R., Li H., Zhou G., Shen C., Zheng G., Zhong G., Li M., Xiao W., Yang S.
    Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
  5. "Signal sequence and keyword trap in silico for selection of full-length human cDNAs encoding secretion or membrane proteins from oligo-capped cDNA libraries."
    Otsuki T., Ota T., Nishikawa T., Hayashi K., Suzuki Y., Yamamoto J., Wakamatsu A., Kimura K., Sakamoto K., Hatano N., Kawai Y., Ishii S., Saito K., Kojima S., Sugiyama T., Ono T., Okano K., Yoshikawa Y.
    , Aotsuka S., Sasaki N., Hattori A., Okumura K., Nagai K., Sugano S., Isogai T.
    DNA Res. 12:117-126(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Embryo.
  6. "The DNA sequence and comparative analysis of human chromosome 20."
    Deloukas P., Matthews L.H., Ashurst J.L., Burton J., Gilbert J.G.R., Jones M., Stavrides G., Almeida J.P., Babbage A.K., Bagguley C.L., Bailey J., Barlow K.F., Bates K.N., Beard L.M., Beare D.M., Beasley O.P., Bird C.P., Blakey S.E.
    , Bridgeman A.M., Brown A.J., Buck D., Burrill W.D., Butler A.P., Carder C., Carter N.P., Chapman J.C., Clamp M., Clark G., Clark L.N., Clark S.Y., Clee C.M., Clegg S., Cobley V.E., Collier R.E., Connor R.E., Corby N.R., Coulson A., Coville G.J., Deadman R., Dhami P.D., Dunn M., Ellington A.G., Frankland J.A., Fraser A., French L., Garner P., Grafham D.V., Griffiths C., Griffiths M.N.D., Gwilliam R., Hall R.E., Hammond S., Harley J.L., Heath P.D., Ho S., Holden J.L., Howden P.J., Huckle E., Hunt A.R., Hunt S.E., Jekosch K., Johnson C.M., Johnson D., Kay M.P., Kimberley A.M., King A., Knights A., Laird G.K., Lawlor S., Lehvaeslaiho M.H., Leversha M.A., Lloyd C., Lloyd D.M., Lovell J.D., Marsh V.L., Martin S.L., McConnachie L.J., McLay K., McMurray A.A., Milne S.A., Mistry D., Moore M.J.F., Mullikin J.C., Nickerson T., Oliver K., Parker A., Patel R., Pearce T.A.V., Peck A.I., Phillimore B.J.C.T., Prathalingam S.R., Plumb R.W., Ramsay H., Rice C.M., Ross M.T., Scott C.E., Sehra H.K., Shownkeen R., Sims S., Skuce C.D., Smith M.L., Soderlund C., Steward C.A., Sulston J.E., Swann R.M., Sycamore N., Taylor R., Tee L., Thomas D.W., Thorpe A., Tracey A., Tromans A.C., Vaudin M., Wall M., Wallis J.M., Whitehead S.L., Whittaker P., Willey D.L., Williams L., Williams S.A., Wilming L., Wray P.W., Hubbard T., Durbin R.M., Bentley D.R., Beck S., Rogers J.
    Nature 414:865-871(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  7. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Brain.

Entry informationi

Entry nameiPIGU_HUMAN
AccessioniPrimary (citable) accession number: Q9H490
Secondary accession number(s): Q7Z489, Q8N2F2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 25, 2003
Last sequence update: January 23, 2007
Last modified: March 16, 2016
This is version 119 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 20
    Human chromosome 20: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.