Q9H490 (PIGU_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphatidylinositol glycan anchor biosynthesis class U protein

Alternative name(s):
Cell division cycle protein 91-like 1
Short name=Protein CDC91-like 1
GPI transamidase component PIG-U

Gene names

Name:	PIGU
Synonyms:	CDC91L1
ORF Names:	PSEC0205, UNQ3055/PRO9875

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	435 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of the GPI transamidase complex. May be involved in the recognition of either the GPI attachment signal or the lipid portion of GPI. Ref.1
Pathway	Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.
Subunit structure	Forms a complex with PIGK/GPI8, PIGS, PIGT and GPAA1/GAA1. Ref.1
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein Potential.
Sequence similarities	Belongs to the PIGU family.

Ontologies

Keywords
Biological process	GPI-anchor biosynthesis
Cellular component	Endoplasmic reticulum Membrane
Coding sequence diversity	Alternative splicing
Domain	Transmembrane Transmembrane helix
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	C-terminal protein lipidation Traceable author statement. Source: Reactome attachment of GPI anchor to protein Inferred from mutant phenotype Ref.1. Source: UniProtKB regulation of JAK-STAT cascade Inferred from direct assay PubMed 15034568. Source: HGNC
Cellular_component	GPI-anchor transamidase complex Inferred from direct assay Ref.1. Source: UniProtKB plasma membrane Inferred from direct assay PubMed 15034568. Source: HGNC
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9H490-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9H490-2)

The sequence of this isoform differs from the canonical sequence as follows:
     66-85: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.1
	Chain	2 – 435	434	Phosphatidylinositol glycan anchor biosynthesis class U protein		PRO_0000121394
Regions
	Transmembrane	66 – 86	21	Helical; Potential
	Transmembrane	166 – 186	21	Helical; Potential
	Transmembrane	188 – 208	21	Helical; Potential
	Transmembrane	237 – 257	21	Helical; Potential
	Transmembrane	259 – 279	21	Helical; Potential
	Transmembrane	286 – 306	21	Helical; Potential
	Transmembrane	313 – 333	21	Helical; Potential
	Transmembrane	355 – 375	21	Helical; Potential
	Transmembrane	386 – 406	21	Helical; Potential
	Region	269 – 289	21	May be involved in recognition of long-chain fatty acids in GPI
Natural variations
	Alternative sequence	66 – 85	20	Missing in isoform 2.		VSP_009543
Experimental info
	Sequence conflict	407	1	Y → N in BAC11660. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 65FDD796AB533027	FASTA	435	50,052
	10 20 30 40 50 60 MAAPLVLVLV VAVTVRAALF RSSLAEFISE RVEVVSPLSS WKRVVEGLSL LDLGVSPYSG 70 80 90 100 110 120 AVFHETPLII YLFHFLIDYA ELVFMITDAL TAIALYFAIQ DFNKVVFKKQ KLLLELDQYA 130 140 150 160 170 180 PDVAELIRTP MEMRYIPLKV ALFYLLNPYT ILSCVAKSTC AINNTLIAFF ILTTIKGSAF 190 200 210 220 230 240 LSAIFLALAT YQSLYPLTLF VPGLLYLLQR QYIPVKMKSK AFWIFSWEYA MMYVGSLVVI 250 260 270 280 290 300 ICLSFFLLSS WDFIPAVYGF ILSVPDLTPN IGLFWYFFAE MFEHFSLFFV CVFQINVFFY 310 320 330 340 350 360 TIPLAIKLKE HPIFFMFIQI AVIAIFKSYP TVGDVALYMA FFPVWNHLYR FLRNIFVLTC 370 380 390 400 410 420 IIIVCSLLFP VLWHLWIYAG SANSNFFYAI TLTFNVGQIL LISDYFYAFL RREYYLTHGL 430 YLTAKDGTEA MLVLK
	Isoform 2 [UniParc]. Checksum: 45BA0934D0273E71	FASTA	415	47,611
	10 20 30 40 50 60 MAAPLVLVLV VAVTVRAALF RSSLAEFISE RVEVVSPLSS WKRVVEGLSL LDLGVSPYSG 70 80 90 100 110 120 AVFHEITDAL TAIALYFAIQ DFNKVVFKKQ KLLLELDQYA PDVAELIRTP MEMRYIPLKV 130 140 150 160 170 180 ALFYLLNPYT ILSCVAKSTC AINNTLIAFF ILTTIKGSAF LSAIFLALAT YQSLYPLTLF 190 200 210 220 230 240 VPGLLYLLQR QYIPVKMKSK AFWIFSWEYA MMYVGSLVVI ICLSFFLLSS WDFIPAVYGF 250 260 270 280 290 300 ILSVPDLTPN IGLFWYFFAE MFEHFSLFFV CVFQINVFFY TIPLAIKLKE HPIFFMFIQI 310 320 330 340 350 360 AVIAIFKSYP TVGDVALYMA FFPVWNHLYR FLRNIFVLTC IIIVCSLLFP VLWHLWIYAG 370 380 390 400 410 SANSNFFYAI TLTFNVGQIL LISDYFYAFL RREYYLTHGL YLTAKDGTEA MLVLK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Human PIG-U and yeast Cdc91p are the fifth subunit of GPI transamidase that attaches GPI-anchors to proteins." Hong Y., Ohishi K., Kang J.Y., Tanaka S., Inoue N., Nishimura J., Maeda Y., Kinoshita T. Mol. Biol. Cell 14:1780-1789(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), PROTEIN SEQUENCE OF 2-8, FUNCTION, SUBUNIT. Tissue: Embryo.
[2]	"PIG-U is a novel oncogene in human bladder cancer." Guo Z., Wu G., Sidransky D., Trink B. Submitted (SEP-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[3]	Lin L., Yu R., Ke R., Li H., Zhou G., Shen C., Zheng G., Zhong G., Li M., Xiao W., Yang S. Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
[4]	"The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment." Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J., Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P., Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E. , Heldens S., Huang A., Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D., Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L., Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C., Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J., Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M. Genome Res. 13:2265-2270(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[5]	"Signal sequence and keyword trap in silico for selection of full-length human cDNAs encoding secretion or membrane proteins from oligo-capped cDNA libraries." Otsuki T., Ota T., Nishikawa T., Hayashi K., Suzuki Y., Yamamoto J., Wakamatsu A., Kimura K., Sakamoto K., Hatano N., Kawai Y., Ishii S., Saito K., Kojima S., Sugiyama T., Ono T., Okano K., Yoshikawa Y. , Aotsuka S., Sasaki N., Hattori A., Okumura K., Nagai K., Sugano S., Isogai T. DNA Res. 12:117-126(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Embryo.
[6]	"The DNA sequence and comparative analysis of human chromosome 20." Deloukas P., Matthews L.H., Ashurst J.L., Burton J., Gilbert J.G.R., Jones M., Stavrides G., Almeida J.P., Babbage A.K., Bagguley C.L., Bailey J., Barlow K.F., Bates K.N., Beard L.M., Beare D.M., Beasley O.P., Bird C.P., Blakey S.E. , Bridgeman A.M., Brown A.J., Buck D., Burrill W.D., Butler A.P., Carder C., Carter N.P., Chapman J.C., Clamp M., Clark G., Clark L.N., Clark S.Y., Clee C.M., Clegg S., Cobley V.E., Collier R.E., Connor R.E., Corby N.R., Coulson A., Coville G.J., Deadman R., Dhami P.D., Dunn M., Ellington A.G., Frankland J.A., Fraser A., French L., Garner P., Grafham D.V., Griffiths C., Griffiths M.N.D., Gwilliam R., Hall R.E., Hammond S., Harley J.L., Heath P.D., Ho S., Holden J.L., Howden P.J., Huckle E., Hunt A.R., Hunt S.E., Jekosch K., Johnson C.M., Johnson D., Kay M.P., Kimberley A.M., King A., Knights A., Laird G.K., Lawlor S., Lehvaeslaiho M.H., Leversha M.A., Lloyd C., Lloyd D.M., Lovell J.D., Marsh V.L., Martin S.L., McConnachie L.J., McLay K., McMurray A.A., Milne S.A., Mistry D., Moore M.J.F., Mullikin J.C., Nickerson T., Oliver K., Parker A., Patel R., Pearce T.A.V., Peck A.I., Phillimore B.J.C.T., Prathalingam S.R., Plumb R.W., Ramsay H., Rice C.M., Ross M.T., Scott C.E., Sehra H.K., Shownkeen R., Sims S., Skuce C.D., Smith M.L., Soderlund C., Steward C.A., Sulston J.E., Swann R.M., Sycamore N., Taylor R., Tee L., Thomas D.W., Thorpe A., Tracey A., Tromans A.C., Vaudin M., Wall M., Wallis J.M., Whitehead S.L., Whittaker P., Willey D.L., Williams L., Williams S.A., Wilming L., Wray P.W., Hubbard T., Durbin R.M., Bentley D.R., Beck S., Rogers J. Nature 414:865-871(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB086842 mRNA. Translation: BAC53626.1. AY422169 mRNA. Translation: AAR23798.1. AY339061 mRNA. Translation: AAQ18022.1. AY358816 mRNA. Translation: AAQ89175.1. AK075507 mRNA. Translation: BAC11660.1. AL118520 Genomic DNA. Translation: CAC14080.1. BC030512 mRNA. Translation: AAH30512.1.
IPI	IPI00026044. IPI00402109.
RefSeq	NP_536724.1. NM_080476.4.
UniGene	Hs.253319.
3D structure databases
ProteinModelPortal	Q9H490.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000217446.
PTM databases
PhosphoSite	Q9H490.
Polymorphism databases
DMDM	29336947.
Proteomic databases
PaxDb	Q9H490.
PRIDE	Q9H490.
Protocols and materials databases
DNASU	128869.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000217446; ENSP00000217446; ENSG00000101464. ENST00000374820; ENSP00000363953; ENSG00000101464.
GeneID	128869.
KEGG	hsa:128869.
UCSC	uc002xas.3. human. uc002xat.3. human.
Organism-specific databases
CTD	128869.
GeneCards	GC20M033148.
HGNC	HGNC:15791. PIGU.
HPA	HPA041706. HPA046766.
MIM	608528. gene.
neXtProt	NX_Q9H490.
PharmGKB	PA162399506.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG297573.
HOGENOM	HOG000189223.
HOVERGEN	HBG082139.
InParanoid	Q9H490.
KO	K05293.
OMA	FFTEMFD.
OrthoDB	EOG4H9XKH.
PhylomeDB	Q9H490.
Enzyme and pathway databases
Reactome	REACT_17015. Metabolism of proteins.
UniPathway	UPA00196.
Gene expression databases
ArrayExpress	Q9H490.
Bgee	Q9H490.
CleanEx	HS_PIGU.
Genevestigator	Q9H490.
GermOnline	ENSG00000101464. Homo sapiens.
Family and domain databases
InterPro	IPR009600. PIG-U. [Graphical view]
PANTHER	PTHR13121. PTHR13121. 1 hit.
Pfam	PF06728. PIG-U. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	PIGU. human.
GenomeRNAi	128869.
NextBio	82506.
SOURCE	Search...

Entry information

Entry name

PIGU_HUMAN

Accession

Primary (citable) accession number: Q9H490
Secondary accession number(s): Q7Z489, Q8N2F2

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 25, 2003
Last sequence update:	January 23, 2007
Last modified:	May 1, 2013
This is version 97 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.