Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

RWD domain-containing protein 1

Gene

RWDD1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Protects DRG2 from proteolytic degradation.By similarity

Names & Taxonomyi

Protein namesi
Recommended name:
RWD domain-containing protein 1
Alternative name(s):
DRG family-regulatory protein 2
Gene namesi
Name:RWDD1
Synonyms:DFRP2
ORF Names:CGI-24, PTD013
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 6

Organism-specific databases

HGNCiHGNC:20993. RWDD1.

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134967923.

Polymorphism and mutation databases

DMDMi34098713.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedCombined sources
Chaini2 – 243242RWD domain-containing protein 1PRO_0000097540Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylthreonineCombined sources
Modified residuei144 – 1441PhosphothreonineCombined sources

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9H446.
MaxQBiQ9H446.
PaxDbiQ9H446.
PRIDEiQ9H446.

PTM databases

iPTMnetiQ9H446.
PhosphoSiteiQ9H446.

Expressioni

Gene expression databases

BgeeiQ9H446.
CleanExiHS_RWDD1.
ExpressionAtlasiQ9H446. baseline and differential.
GenevisibleiQ9H446. HS.

Organism-specific databases

HPAiHPA028712.
HPA028713.

Interactioni

Subunit structurei

Interacts with DRG2. Interacts with androgen receptor (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
DRG1Q9Y2955EBI-748952,EBI-719554
DRG2P550394EBI-748952,EBI-750565

Protein-protein interaction databases

BioGridi119518. 4 interactions.
DIPiDIP-34520N.
IntActiQ9H446. 5 interactions.
MINTiMINT-1436573.
STRINGi9606.ENSP00000420357.

Structurei

Secondary structure

1
243
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi4 – 1815Combined sources
Beta strandi20 – 256Combined sources
Beta strandi28 – 303Combined sources
Beta strandi32 – 354Combined sources
Beta strandi49 – 557Combined sources
Turni60 – 623Combined sources
Beta strandi66 – 749Combined sources
Helixi77 – 9418Combined sources
Helixi100 – 11718Combined sources
Helixi118 – 1203Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2EBMNMR-A1-121[»]
ProteinModelPortaliQ9H446.
SMRiQ9H446. Positions 1-121.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9H446.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini10 – 114105RWDPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni142 – 19756Interaction with DRG2By similarityAdd
BLAST

Sequence similaritiesi

Belongs to the RWDD1/GIR2 family.Curated
Contains 1 RWD domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG4018. Eukaryota.
ENOG410XT0M. LUCA.
GeneTreeiENSGT00390000009168.
HOGENOMiHOG000237961.
HOVERGENiHBG060864.
InParanoidiQ9H446.
OMAiPPLWEIY.
OrthoDBiEOG779NZV.
PhylomeDBiQ9H446.
TreeFamiTF313662.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR006575. RWD-domain.
IPR016135. UBQ-conjugating_enzyme/RWD.
IPR032378. ZC3H15/TMA46_C.
[Graphical view]
PfamiPF16543. DFRP_C. 1 hit.
PF05773. RWD. 1 hit.
[Graphical view]
SMARTiSM00591. RWD. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiPS50908. RWD. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9H446-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTDYGEEQRN ELEALESIYP DSFTVLSENP PSFTITVTSE AGENDETVQT
60 70 80 90 100
TLKFTYSEKY PDEAPLYEIF SQENLEDNDV SDILKLLALQ AEENLGMVMI
110 120 130 140 150
FTLVTAVQEK LNEIVDQIKT RREEEKKQKE KEAEEAEKQL FHGTPVTIEN
160 170 180 190 200
FLNWKAKFDA ELLEIKKKRM KEEEQAGKNK LSGKQLFETD HNLDTSDIQF
210 220 230 240
LEDAGNNVEV DESLFQEMDD LELEDDEDDP DYNPADPESD SAD
Length:243
Mass (Da):27,940
Last modified:March 1, 2001 - v1
Checksum:iA7AABD457136B4DA
GO
Isoform 2 (identifier: Q9H446-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-96: Missing.

Show »
Length:147
Mass (Da):17,082
Checksum:iA2927D314AE84CE2
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti120 – 13011TRREEEKKQKE → LEERRKNKR in AAD27733 (PubMed:10810093).CuratedAdd
BLAST
Sequence conflicti139 – 1391Q → R in AAD40376 (Ref. 2) Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 9696Missing in isoform 2. 1 PublicationVSP_044500Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF132958 mRNA. Translation: AAD27733.1.
AF092134 mRNA. Translation: AAD40376.1.
AK290727 mRNA. Translation: BAF83416.1.
AK301089 mRNA. Translation: BAG62691.1.
AL121953 Genomic DNA. Translation: CAC14427.1.
CH471051 Genomic DNA. Translation: EAW48221.1.
CH471051 Genomic DNA. Translation: EAW48222.1.
BC015802 mRNA. Translation: AAH15802.1.
CCDSiCCDS34520.1. [Q9H446-1]
CCDS43496.1. [Q9H446-2]
RefSeqiNP_001007465.1. NM_001007464.2. [Q9H446-2]
NP_057036.2. NM_015952.3. [Q9H446-1]
NP_057188.2. NM_016104.3. [Q9H446-2]
UniGeneiHs.532164.

Genome annotation databases

EnsembliENST00000466444; ENSP00000420357; ENSG00000111832. [Q9H446-1]
ENST00000487832; ENSP00000428778; ENSG00000111832. [Q9H446-2]
GeneIDi51389.
KEGGihsa:51389.
UCSCiuc003pxc.5. human. [Q9H446-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF132958 mRNA. Translation: AAD27733.1.
AF092134 mRNA. Translation: AAD40376.1.
AK290727 mRNA. Translation: BAF83416.1.
AK301089 mRNA. Translation: BAG62691.1.
AL121953 Genomic DNA. Translation: CAC14427.1.
CH471051 Genomic DNA. Translation: EAW48221.1.
CH471051 Genomic DNA. Translation: EAW48222.1.
BC015802 mRNA. Translation: AAH15802.1.
CCDSiCCDS34520.1. [Q9H446-1]
CCDS43496.1. [Q9H446-2]
RefSeqiNP_001007465.1. NM_001007464.2. [Q9H446-2]
NP_057036.2. NM_015952.3. [Q9H446-1]
NP_057188.2. NM_016104.3. [Q9H446-2]
UniGeneiHs.532164.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2EBMNMR-A1-121[»]
ProteinModelPortaliQ9H446.
SMRiQ9H446. Positions 1-121.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi119518. 4 interactions.
DIPiDIP-34520N.
IntActiQ9H446. 5 interactions.
MINTiMINT-1436573.
STRINGi9606.ENSP00000420357.

PTM databases

iPTMnetiQ9H446.
PhosphoSiteiQ9H446.

Polymorphism and mutation databases

DMDMi34098713.

Proteomic databases

EPDiQ9H446.
MaxQBiQ9H446.
PaxDbiQ9H446.
PRIDEiQ9H446.

Protocols and materials databases

DNASUi51389.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000466444; ENSP00000420357; ENSG00000111832. [Q9H446-1]
ENST00000487832; ENSP00000428778; ENSG00000111832. [Q9H446-2]
GeneIDi51389.
KEGGihsa:51389.
UCSCiuc003pxc.5. human. [Q9H446-1]

Organism-specific databases

CTDi51389.
GeneCardsiRWDD1.
HGNCiHGNC:20993. RWDD1.
HPAiHPA028712.
HPA028713.
neXtProtiNX_Q9H446.
PharmGKBiPA134967923.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG4018. Eukaryota.
ENOG410XT0M. LUCA.
GeneTreeiENSGT00390000009168.
HOGENOMiHOG000237961.
HOVERGENiHBG060864.
InParanoidiQ9H446.
OMAiPPLWEIY.
OrthoDBiEOG779NZV.
PhylomeDBiQ9H446.
TreeFamiTF313662.

Miscellaneous databases

ChiTaRSiRWDD1. human.
EvolutionaryTraceiQ9H446.
GenomeRNAii51389.
PROiQ9H446.

Gene expression databases

BgeeiQ9H446.
CleanExiHS_RWDD1.
ExpressionAtlasiQ9H446. baseline and differential.
GenevisibleiQ9H446. HS.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR006575. RWD-domain.
IPR016135. UBQ-conjugating_enzyme/RWD.
IPR032378. ZC3H15/TMA46_C.
[Graphical view]
PfamiPF16543. DFRP_C. 1 hit.
PF05773. RWD. 1 hit.
[Graphical view]
SMARTiSM00591. RWD. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiPS50908. RWD. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification of novel human genes evolutionarily conserved in Caenorhabditis elegans by comparative proteomics."
    Lai C.-H., Chou C.-Y., Ch'ang L.-Y., Liu C.-S., Lin W.-C.
    Genome Res. 10:703-713(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
  2. Song H., Peng Y., Dai M., Huang Q., Mao Y., Zhang Q., Mao M., Fu G., Luo M., Chen J., Hu R.
    Submitted (SEP-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Pituitary tumor.
  3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
  4. "The DNA sequence and analysis of human chromosome 6."
    Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D.
    , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J., French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S.
    Nature 425:805-811(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Uterus.
  7. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
    Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
    Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-144, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  8. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT THR-2, CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS], IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  10. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."
    Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L., Ye M., Zou H.
    J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  11. "Solution structure of the RWD domain of human RWD domain containing protein 1."
    RIKEN structural genomics initiative (RSGI)
    Submitted (AUG-2007) to the PDB data bank
    Cited for: STRUCTURE BY NMR OF 1-121.

Entry informationi

Entry nameiRWDD1_HUMAN
AccessioniPrimary (citable) accession number: Q9H446
Secondary accession number(s): A8K3W2
, A8MT24, Q9Y313, Q9Y6B3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 15, 2003
Last sequence update: March 1, 2001
Last modified: June 8, 2016
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 6
    Human chromosome 6: entries, gene names and cross-references to MIM
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.