Q9H427 (KCNKF_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Potassium channel subfamily K member 15

Alternative name(s):
Acid-sensitive potassium channel protein TASK-5
TWIK-related acid-sensitive K(+) channel 5
Two pore potassium channel KT3.3
Short name=Two pore K(+) channel KT3.3

Gene names

Name:	KCNK15
Synonyms:	TASK5

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	330 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Probable potassium channel subunit. No channel activity observed in heterologous systems. May need to associate with another protein to form a functional channel.
Subunit structure	Heterodimer Potential.
Subcellular location	Membrane; Multi-pass membrane protein.
Tissue specificity	Detected in pancreas, heart, placenta, lung, liver, kidney, ovary, testis, skeletal muscle and adrenal gland, and at lower levels in prostate, spleen and thyroid gland. Ref.1 Ref.4
Polymorphism	Three variant polypeptides are known: TASK-5A, TASK-5B and TASK-5C. The sequence shown is that of TASK-5C.
Sequence similarities	Belongs to the two pore domain potassium channel (TC 1.A.1.8) family. [View classification]

Ontologies

Keywords
Biological process	Ion transport Potassium transport Transport
Cellular component	Membrane
Coding sequence diversity	Polymorphism
Domain	Transmembrane Transmembrane helix
Ligand	Potassium
Molecular function	Ion channel Potassium channel Voltage-gated channel
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	regulation of ion transmembrane transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	potassium channel activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 330	330	Potassium channel subfamily K member 15		PRO_0000101765
Regions
	Topological domain	1 – 8	8	Cytoplasmic Potential
	Transmembrane	9 – 29	21	Helical; Potential
	Intramembrane	80 – 101	22	Pore-forming; Name=Pore-forming 1; Potential
	Transmembrane	108 – 128	21	Helical; Potential
	Topological domain	129 – 158	30	Cytoplasmic Potential
	Transmembrane	159 – 179	21	Helical; Potential
	Intramembrane	189 – 209	21	Pore-forming; Name=Pore-forming 2; Potential
	Transmembrane	223 – 243	21	Helical; Potential
	Topological domain	244 – 330	87	Cytoplasmic Potential
Natural variations
	Natural variant	95	1	G → E in TASK-5B. Ref.1 Ref.3 Ref.5 Ref.6 Corresponds to variant rs1111032 [ dbSNP | Ensembl ].		VAR_014211
	Natural variant	260	1	P → T in TASK-5B. Ref.3 Ref.5 Ref.6 Corresponds to variant rs6073538 [ dbSNP | Ensembl ].		VAR_014212
	Natural variant	261	1	P → H in TASK-5A. Ref.3 Ref.6 Corresponds to variant rs13037900 [ dbSNP | Ensembl ].		VAR_014213
	Natural variant	323	1	P → L in TASK-5B. Ref.3 Ref.5 Corresponds to variant rs13042905 [ dbSNP | Ensembl ].		VAR_014214
Experimental info
	Mutagenesis	138	1	R → Y: No effect on lack of functional expression. Ref.3
	Mutagenesis	141 – 145	5	LAAKC → HRAKK: No effect on lack of functional expression. Ref.3
	Mutagenesis	151	1	W → R: No effect on lack of functional expression. Ref.3
	Mutagenesis	153	1	C → D: No effect on lack of functional expression. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q9H427 [UniParc]. Last modified November 1, 2002. Version 2. Checksum: AA2A54D0615BC53C	FASTA	330	36,130
	10 20 30 40 50 60 MRRPSVRAAG LVLCTLCYLL VGAAVFDALE SEAESGRQRL LVQKRGALRR KFGFSAEDYR 70 80 90 100 110 120 ELERLALQAE PHRAGRQWKF PGSFYFAITV ITTIGYGHAA PGTDSGKVFC MFYALLGIPL 130 140 150 160 170 180 TLVTFQSLGE RLNAVVRRLL LAAKCCLGLR WTCVSTENLV VAGLLACAAT LALGAVAFSH 190 200 210 220 230 240 FEGWTFFHAY YYCFITLTTI GFGDFVALQS GEALQRKLPY VAFSFLYILL GLTVIGAFLN 250 260 270 280 290 300 LVVLRFLVAS ADWPERAARP PSPRPPGAPE SRGLWLPRRP ARSVGSASVF CHVHKLERCA 310 320 330 RDNLGFSPPS SPGVVRGGQA PRPGARWKSI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"TASK-5, a new member of the tandem-pore K(+) channel family." Kim D., Gnatenco C. Biochem. Biophys. Res. Commun. 284:923-930(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, VARIANT GLU-95.
[2]	"KT3.2 and KT3.3, two novel human two-pore K(+) channels closely related to TASK-1." Vega-Saenz de Miera E., Lau D.H.P., Zhadina M., Pountney D., Coetzee W.A., Rudy B. J. Neurophysiol. 86:130-142(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain.
[3]	"Expression pattern in brain of TASK-1, TASK-3, and a tandem pore domain K(+) channel subunit, TASK-5, associated with the central auditory nervous system." Karschin C., Wischmeyer E., Preisig-Mueller R., Rajan S., Derst C., Grzeschik K.-H., Daut J., Karschin A. Mol. Cell. Neurosci. 18:632-648(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], POLYMORPHISM, VARIANTS GLU-95; THR-260; HIS-261 AND LEU-323, MUTAGENESIS OF ARG-138; 141-LEU--CYS-145; TRP-151 AND CYS-153. Tissue: Brain, Colon and Ovarian carcinoma.
[4]	"TASK-5, a novel member of the tandem pore K+ channel family." Ashmole I., Goodwin P.A., Stanfield P.R. Pflugers Arch. 442:828-833(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
[5]	"The DNA sequence and comparative analysis of human chromosome 20." Deloukas P., Matthews L.H., Ashurst J.L., Burton J., Gilbert J.G.R., Jones M., Stavrides G., Almeida J.P., Babbage A.K., Bagguley C.L., Bailey J., Barlow K.F., Bates K.N., Beard L.M., Beare D.M., Beasley O.P., Bird C.P., Blakey S.E. , Bridgeman A.M., Brown A.J., Buck D., Burrill W.D., Butler A.P., Carder C., Carter N.P., Chapman J.C., Clamp M., Clark G., Clark L.N., Clark S.Y., Clee C.M., Clegg S., Cobley V.E., Collier R.E., Connor R.E., Corby N.R., Coulson A., Coville G.J., Deadman R., Dhami P.D., Dunn M., Ellington A.G., Frankland J.A., Fraser A., French L., Garner P., Grafham D.V., Griffiths C., Griffiths M.N.D., Gwilliam R., Hall R.E., Hammond S., Harley J.L., Heath P.D., Ho S., Holden J.L., Howden P.J., Huckle E., Hunt A.R., Hunt S.E., Jekosch K., Johnson C.M., Johnson D., Kay M.P., Kimberley A.M., King A., Knights A., Laird G.K., Lawlor S., Lehvaeslaiho M.H., Leversha M.A., Lloyd C., Lloyd D.M., Lovell J.D., Marsh V.L., Martin S.L., McConnachie L.J., McLay K., McMurray A.A., Milne S.A., Mistry D., Moore M.J.F., Mullikin J.C., Nickerson T., Oliver K., Parker A., Patel R., Pearce T.A.V., Peck A.I., Phillimore B.J.C.T., Prathalingam S.R., Plumb R.W., Ramsay H., Rice C.M., Ross M.T., Scott C.E., Sehra H.K., Shownkeen R., Sims S., Skuce C.D., Smith M.L., Soderlund C., Steward C.A., Sulston J.E., Swann R.M., Sycamore N., Taylor R., Tee L., Thomas D.W., Thorpe A., Tracey A., Tromans A.C., Vaudin M., Wall M., Wallis J.M., Whitehead S.L., Whittaker P., Willey D.L., Williams L., Williams S.A., Wilming L., Wray P.W., Hubbard T., Durbin R.M., Bentley D.R., Beck S., Rogers J. Nature 414:865-871(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], VARIANTS GLU-95; THR-260 AND LEU-323.
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANTS GLU-95; THR-260 AND HIS-261. Tissue: Brain.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF257081 mRNA. Translation: AAG33127.1. AF294350 mRNA. Translation: AAK97091.1. AF294351 mRNA. Translation: AAK97092.1. AF294352 Genomic DNA. Translation: AAK97093.1. AF336342 mRNA. Translation: AAK37518.1. AL118522 Genomic DNA. Translation: CAC14068.1. BC093874 mRNA. Translation: AAH93874.1. BC143282 mRNA. Translation: AAI43283.1.
IPI	IPI00007134.
PIR	JC7703.
RefSeq	NP_071753.2. NM_022358.3.
UniGene	Hs.528664.
3D structure databases
ProteinModelPortal	Q9H427.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000361952.
Polymorphism databases
DMDM	24636282.
Proteomic databases
PaxDb	Q9H427.
PRIDE	Q9H427.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000372861; ENSP00000361952; ENSG00000124249.
GeneID	60598.
KEGG	hsa:60598.
UCSC	uc002xmr.3. human.
Organism-specific databases
CTD	60598.
GeneCards	GC20P043374.
H-InvDB	HIX0040517.
HGNC	HGNC:13814. KCNK15.
MIM	607368. gene.
neXtProt	NX_Q9H427.
PharmGKB	PA30056.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG259548.
HOGENOM	HOG000231463.
HOVERGEN	HBG052239.
InParanoid	Q9H427.
KO	K04923.
OrthoDB	EOG4VX25V.
Gene expression databases
Bgee	Q9H427.
CleanEx	HS_KCNK15.
Genevestigator	Q9H427.
Family and domain databases
InterPro	IPR003280. 2pore_dom_K_chnl. IPR003092. 2pore_dom_K_chnl_TASK. IPR022306. 2pore_dom_K_chnl_TASK/TWIK. IPR008073. 2pore_dom_K_chnl_TASK5. IPR013099. Ion_trans_2. [Graphical view]
PANTHER	PTHR11003:SF18. PTHR11003:SF18. 1 hit.
Pfam	PF07885. Ion_trans_2. 2 hits. [Graphical view]
PIRSF	PIRSF038061. K_channel_subfamily_K_type. 1 hit.
PRINTS	PR01333. 2POREKCHANEL. PR01690. TASK5CHANNEL. PR01095. TASKCHANNEL.
ProtoNet	Search...
Other
GenomeRNAi	60598.
NextBio	65457.
SOURCE	Search...

Entry information

Entry name

KCNKF_HUMAN

Accession

Primary (citable) accession number: Q9H427
Secondary accession number(s): Q52LL3, Q9HBC8

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 2002
Last sequence update:	November 1, 2002
Last modified:	May 1, 2013
This is version 93 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.