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Reviewed, UniProtKB/Swiss-Prot Q9H3S4 (TPK1_HUMAN)

Last modified November 3, 2009. Version 66. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Thiamin pyrophosphokinase 1
    EC=2.7.6.2
Alternative name(s):
    Thiamine pyrophosphokinase 1
      Short name=hTPK1
    Placental protein 20
      Short name=PP20
Gene names
Name: TPK1
OrganismHomo sapiens (Human) [Complete proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length243 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at transcript level.

General annotation (Comments)

Function

Catalyzes the phosphorylation of thiamine to thiamine pyrophosphate. Can also catalyze the phosphorylation of pyrithiamine to pyrithiamine pyrophosphate. Ref.1

Catalytic activity

ATP + thiamine = AMP + thiamine diphosphate.

Pathway

Cofactor biosynthesis; thiamine diphosphate biosynthesis; thiamine diphosphate from thiamine: step 1/1.

Subunit structure

Homodimer.

Tissue specificity

Detected in heart, kidney, testis, small intestine and peripheral blood leukocytes, and at very low levels in a variety of tissues. Ref.1 Ref.2

Sequence similarities

Belongs to the thiamine pyrophosphokinase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 243243Thiamin pyrophosphokinase 1
PRO_0000072647

Sequences

Sequence LengthMass (Da)Tools
Q9H3S4-1 [UniParc].

Last modified March 1, 2001. Version 1.
Checksum: F7E96F8127CB4FA5

FASTA24327,265
        10         20         30         40         50         60 
MEHAFTPLEP LLSTGNLKYC LVILNQPLDN YFRHLWNKAL LRACADGGAN RLYDITEGER 

        70         80         90        100        110        120 
ESFLPEFING DFDSIRPEVR EYYATKGCEL ISTPDQDHTD FTKCLKMLQK KIEEKDLKVD 

       130        140        150        160        170        180 
VIVTLGGLAG RFDQIMASVN TLFQATHITP FPIIIIQEES LIYLLQPGKH RLHVDTGMEG 

       190        200        210        220        230        240 
DWCGLIPVGQ PCMQVTTTGL KWNLTNDVLA FGTLVSTSNT YDGSGVVTVE TDHPLLWTMA 


IKS 

« Hide

References

« Hide 'large scale' references
[1]"Isolation and characterization of a human thiamine pyrophosphokinase cDNA."
Nosaka K., Onozuka M., Kakazu N., Hibi S., Nishimura H., Nishino H., Abe T.
Biochim. Biophys. Acta 1517:293-297(2001) [PubMed: 11342111] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY.
Tissue: Liver.
[2]"Molecular cloning of human thiamin pyrophosphokinase."
Zhao R., Gao F., Goldman I.D.
Biochim. Biophys. Acta 1517:320-322(2001) [PubMed: 11342117] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
Tissue: Liver.
[3]"Cloning, sequencing, structural and molecular biological characterization of placental protein 20 (PP20)/human thiamin pyrophosphokinase (hTPK)."
Bellyei S., Szigeti A., Boronkai A., Szabo Z., Bene J., Janaky T., Barna L., Sipos K., Minik O., Kravjak A., Ohmacht R., Melegh B., Zavodszky P., Than G.N., Sumegi B., Bohn H., Than N.G.
Placenta 26:34-46(2005) [PubMed: 15664409] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 11-243.
Tissue: Pancreas.
[5]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 31-243.
Tissue: Small intestine.
+Additional computationally mapped references.

Cross-references

Sequence databases

AB028138 mRNA. Translation: BAB20326.1.
AF297710 mRNA. Translation: AAK01351.1.
AY206415 mRNA. Translation: AAO38775.1.
BC014552 mRNA. Translation: AAH14552.2.
AK026374 mRNA. Translation: BAB15465.1. Different initiation.
IPIIPI00072523.
RefSeqNP_071890.2.
UniGeneHs.660232

3D structure databases

EntryMethodResolution (Å)ChainPositionsPDBsum
1OLYmodel-A/B1-243[»]
SMRQ9H3S4. Positions 1-243.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ9H3S4.

Proteomic databases

PRIDEQ9H3S4.

Genome annotation databases

EnsemblENST00000360057; ENSP00000353165; ENSG00000196511; Homo sapiens. [Genome view]
ENST00000378098; ENSP00000367338; ENSG00000196511; Homo sapiens. [Genome view]
ENST00000378099; ENSP00000367339; ENSG00000196511; Homo sapiens. [Genome view]
GeneID27010.
UCSCuc003weq.1. human.

Organism-specific databases

CTD27010.
GeneCardsGC07M143779.
H-InvDBHIX0007177.
HGNCHGNC:17358. TPK1.
HPAHPA021545.
HPA021849.
MIM606370. gene.
PharmGKBPA29974.
GenAtlasSearch...

Phylogenomic databases

HOGENOMQ9H3S4.
HOVERGENQ9H3S4.
OMACNQVTTT.

Enzyme and pathway databases

BRENDA2.7.6.2. 247.
ReactomeREACT_11193. Metabolism of vitamins and cofactors.

Gene expression databases

ArrayExpressQ9H3S4.
BgeeQ9H3S4.
CleanExHS_TPK1.
GenevestigatorQ9H3S4.
GermOnlineENSG00000196511. Homo sapiens.

Family and domain databases

InterProIPR006282. Thi_PPkinase.
IPR016966. Thiamin_pyrophosphokinase_euk.
IPR007373. TPK_B1_bd.
IPR007371. TPK_catalytic.
[Graphical view]
Gene3DG3DSA:3.40.50.10240. TPK_catalytic. 1 hit.
PfamPF04265. TPK_B1_binding. 1 hit.
PF04263. TPK_catalytic. 1 hit.
[Graphical view]
PIRSFPIRSF031057. Thiamin_pyrophosphokinase. 1 hit.
TIGRFAMsTIGR01378. thi_PPkinase. 1 hit.
ProtoNetSearch...

Other Resources

DrugBankDB00152. Thiamine.
NextBio49516.
SOURCESearch...

Entry information

Entry nameTPK1_HUMAN
AccessionPrimary (citable) accession number: Q9H3S4
Secondary accession number(s): Q9H602
Entry history
Integrated into UniProtKB/Swiss-Prot: March 1, 2004
Last sequence update: March 1, 2001
Last modified: November 3, 2009
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

Human chromosome 7

Human chromosome 7: entries, gene names and cross-references to MIM

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

PATHWAY comments

Index of metabolic and biosynthesis pathways

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents