Reviewed, UniProtKB/Swiss-Prot Q9H3S4 (TPK1_HUMAN)

Names and origin

Protein names

Recommended name:
    Thiamin pyrophosphokinase 1
    EC=2.7.6.2
Alternative name(s):
    Thiamine pyrophosphokinase 1
      Short name=hTPK1
    Placental protein 20
      Short name=PP20

Gene names

Name:

TPK1

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	243 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the phosphorylation of thiamine to thiamine pyrophosphate. Can also catalyze the phosphorylation of pyrithiamine to pyrithiamine pyrophosphate. Ref.1
Catalytic activity	ATP + thiamine = AMP + thiamine diphosphate.
Pathway	Cofactor biosynthesis; thiamine diphosphate biosynthesis; thiamine diphosphate from thiamine: step 1/1.
Subunit structure	Homodimer.
Tissue specificity	Detected in heart, kidney, testis, small intestine and peripheral blood leukocytes, and at very low levels in a variety of tissues. Ref.1 Ref.2
Sequence similarities	Belongs to the thiamine pyrophosphokinase family.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	thiamin diphosphate biosynthetic process Inferred from electronic annotation. Source: InterPro thiamin metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW kinase activity Inferred from electronic annotation. Source: UniProtKB-KW thiamin diphosphokinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 243	243	Thiamin pyrophosphokinase 1		PRO_0000072647

Sequences

	Sequence		Length	Mass (Da)
	Q9H3S4-1 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: F7E96F8127CB4FA5	FASTA	243	27,265
	10 20 30 40 50 60 MEHAFTPLEP LLSTGNLKYC LVILNQPLDN YFRHLWNKAL LRACADGGAN RLYDITEGER 70 80 90 100 110 120 ESFLPEFING DFDSIRPEVR EYYATKGCEL ISTPDQDHTD FTKCLKMLQK KIEEKDLKVD 130 140 150 160 170 180 VIVTLGGLAG RFDQIMASVN TLFQATHITP FPIIIIQEES LIYLLQPGKH RLHVDTGMEG 190 200 210 220 230 240 DWCGLIPVGQ PCMQVTTTGL KWNLTNDVLA FGTLVSTSNT YDGSGVVTVE TDHPLLWTMA IKS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation and characterization of a human thiamine pyrophosphokinase cDNA." Nosaka K., Onozuka M., Kakazu N., Hibi S., Nishimura H., Nishino H., Abe T. Biochim. Biophys. Acta 1517:293-297(2001) [PubMed: 11342111] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY. Tissue: Liver.
[2]	"Molecular cloning of human thiamin pyrophosphokinase." Zhao R., Gao F., Goldman I.D. Biochim. Biophys. Acta 1517:320-322(2001) [PubMed: 11342117] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY. Tissue: Liver.
[3]	"Cloning, sequencing, structural and molecular biological characterization of placental protein 20 (PP20)/human thiamin pyrophosphokinase (hTPK)." Bellyei S., Szigeti A., Boronkai A., Szabo Z., Bene J., Janaky T., Barna L., Sipos K., Minik O., Kravjak A., Ohmacht R., Melegh B., Zavodszky P., Than G.N., Sumegi B., Bohn H., Than N.G. Placenta 26:34-46(2005) [PubMed: 15664409] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 11-243. Tissue: Pancreas.
[5]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 31-243. Tissue: Small intestine.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

AB028138 mRNA. Translation: BAB20326.1.
AF297710 mRNA. Translation: AAK01351.1.
AY206415 mRNA. Translation: AAO38775.1.
BC014552 mRNA. Translation: AAH14552.2.
AK026374 mRNA. Translation: BAB15465.1. Different initiation.

IPI

IPI00072523.

RefSeq

NP_071890.2.

UniGene

Hs.660232

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1OLY	model	-	A/B	1-243	[»]

SMR

Q9H3S4. Positions 1-243.

ModBase

Search...

Protein-protein interaction databases

STRING

Q9H3S4.

Proteomic databases

PRIDE

Q9H3S4.

Genome annotation databases

Ensembl

ENST00000360057; ENSP00000353165; ENSG00000196511; Homo sapiens. [Genome view]
ENST00000378098; ENSP00000367338; ENSG00000196511; Homo sapiens. [Genome view]
ENST00000378099; ENSP00000367339; ENSG00000196511; Homo sapiens. [Genome view]

GeneID

27010.

UCSC

uc003weq.1. human.

Organism-specific databases

CTD

27010.

GeneCards

GC07M143779.

H-InvDB

HIX0007177.

HGNC

HGNC:17358. TPK1.

HPA

HPA021545.
HPA021849.

MIM

606370. gene.

PharmGKB

PA29974.

GenAtlas

Search...

Phylogenomic databases

HOGENOM

Q9H3S4.

HOVERGEN

Q9H3S4.

OMA

CNQVTTT.

Enzyme and pathway databases

BRENDA

2.7.6.2. 247.

Reactome

REACT_11193. Metabolism of vitamins and cofactors.

Gene expression databases

ArrayExpress

Q9H3S4.

Bgee

Q9H3S4.

CleanEx

HS_TPK1.

Genevestigator

Q9H3S4.

GermOnline

ENSG00000196511. Homo sapiens.

Family and domain databases

InterPro

IPR006282. Thi_PPkinase.
IPR016966. Thiamin_pyrophosphokinase_euk.
IPR007373. TPK_B1_bd.
IPR007371. TPK_catalytic.
[Graphical view]

Gene3D

G3DSA:3.40.50.10240. TPK_catalytic. 1 hit.

Pfam

PF04265. TPK_B1_binding. 1 hit.
PF04263. TPK_catalytic. 1 hit.
[Graphical view]

PIRSF

PIRSF031057. Thiamin_pyrophosphokinase. 1 hit.

TIGRFAMs

TIGR01378. thi_PPkinase. 1 hit.

ProtoNet

Search...

Other Resources

DrugBank

DB00152. Thiamine.

NextBio

49516.

SOURCE

Search...

Entry information

Entry name

TPK1_HUMAN

Accession

Primary (citable) accession number: Q9H3S4
Secondary accession number(s): Q9H602

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 2004
Last sequence update:	March 1, 2001
Last modified:	November 3, 2009
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.