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Q9H3R5

- CENPH_HUMAN

UniProt

Q9H3R5 - CENPH_HUMAN

Protein

Centromere protein H

Gene

CENPH

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 109 (01 Oct 2014)
      Sequence version 1 (01 Mar 2001)
      Previous versions | rss
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    Functioni

    Component of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres. Required for chromosome congression and efficiently align the chromosomes on a metaphase plate.3 Publications

    GO - Molecular functioni

    1. kinetochore binding Source: UniProtKB
    2. protein binding Source: UniProtKB

    GO - Biological processi

    1. CENP-A containing nucleosome assembly Source: Reactome
    2. kinetochore assembly Source: InterPro
    3. kinetochore organization Source: UniProtKB
    4. mitotic cell cycle Source: Reactome
    5. nucleosome assembly Source: Reactome

    Enzyme and pathway databases

    ReactomeiREACT_150425. Resolution of Sister Chromatid Cohesion.
    REACT_150471. Separation of Sister Chromatids.
    REACT_22186. Deposition of new CENPA-containing nucleosomes at the centromere.
    REACT_682. Mitotic Prometaphase.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Centromere protein H
    Short name:
    CENP-H
    Alternative name(s):
    Interphase centromere complex protein 35
    Gene namesi
    Name:CENPHImported
    Synonyms:ICEN35
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 5

    Organism-specific databases

    HGNCiHGNC:17268. CENPH.

    Subcellular locationi

    Nucleus. Chromosomecentromerekinetochore
    Note: Associates with active centromere-kinetochore complexes throughout the cell cycle. Colocalizes with inner kinetochore plate proteins CENPA and CENPC during both interphase and metaphase.

    GO - Cellular componenti

    1. condensed chromosome kinetochore Source: UniProtKB-SubCell
    2. cytosol Source: Reactome
    3. kinetochore Source: UniProtKB
    4. nucleoplasm Source: Reactome
    5. nucleus Source: UniProtKB

    Keywords - Cellular componenti

    Centromere, Chromosome, Kinetochore, Nucleus

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA26402.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 247247Centromere protein HPRO_0000089478Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-acetylmethionine2 Publications

    Keywords - PTMi

    Acetylation

    Proteomic databases

    MaxQBiQ9H3R5.
    PaxDbiQ9H3R5.
    PRIDEiQ9H3R5.

    PTM databases

    PhosphoSiteiQ9H3R5.

    Expressioni

    Gene expression databases

    ArrayExpressiQ9H3R5.
    BgeeiQ9H3R5.
    CleanExiHS_CENPH.
    GenevestigatoriQ9H3R5.

    Organism-specific databases

    HPAiHPA036494.

    Interactioni

    Subunit structurei

    Self-associates. Component of the CENPA-NAC complex, at least composed of CENPA, CENPC, CENPH, CENPM, CENPN, CENPT and CENPU. The CENPA-NAC complex interacts with the CENPA-CAD complex, composed of CENPI, CENPK, CENPL, CENPO, CENPP, CENPQ, CENPR and CENPS. Interacts directly with CENPK. Interacts with KIF2C and NDC80. Interacts with TRIM36 By similarity.By similarity

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    NDC80O147773EBI-1003700,EBI-715849

    Protein-protein interaction databases

    BioGridi122355. 17 interactions.
    IntActiQ9H3R5. 1 interaction.
    MINTiMINT-4988967.
    STRINGi9606.ENSP00000283006.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9H3R5.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili47 – 192146Sequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Belongs to the centromere protein H family.Sequence Analysis

    Keywords - Domaini

    Coiled coil

    Phylogenomic databases

    eggNOGiNOG10470.
    HOGENOMiHOG000013068.
    HOVERGENiHBG081083.
    InParanoidiQ9H3R5.
    KOiK11500.
    OMAiQMEIKIT.
    OrthoDBiEOG7JQBRQ.
    PhylomeDBiQ9H3R5.
    TreeFamiTF101134.

    Family and domain databases

    InterProiIPR008426. CENP-H.
    [Graphical view]
    PfamiPF05837. CENP-H. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9H3R5-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEEQPQMQDA DEPADSGGEG RAGGPPQVAG AQAACSEDRM TLLLRLRAQT    50
    KQQLLEYKSM VDASEEKTPE QIMQEKQIEA KIEDLENEIE EVKVAFEIKK 100
    LALDRMRLST ALKKNLEKIS RQSSVLMDNM KHLLELNKLI MKSQQESWDL 150
    EEKLLDIRKK RLQLKQASES KLLEIQTEKN KQKIDLDSME NSERIKIIRQ 200
    NLQMEIKITT VIQHVFQNLI LGSKVNWAED PALKEIVLQL EKNVDMM 247
    Length:247
    Mass (Da):28,481
    Last modified:March 1, 2001 - v1
    Checksum:iE1BCD4022710168F
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti2 – 21E → K in a colorectal cancer sample; somatic mutation. 1 Publication
    VAR_036167

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB035124 mRNA. Translation: BAB18635.1.
    AK290746 mRNA. Translation: BAF83435.1.
    CH471137 Genomic DNA. Translation: EAW51304.1.
    BC015355 mRNA. Translation: AAH15355.1.
    BC012024 mRNA. Translation: AAH12024.1.
    CCDSiCCDS3998.1.
    RefSeqiNP_075060.1. NM_022909.3.
    UniGeneiHs.631967.

    Genome annotation databases

    EnsembliENST00000283006; ENSP00000283006; ENSG00000153044.
    GeneIDi64946.
    KEGGihsa:64946.
    UCSCiuc003jvp.3. human.

    Polymorphism databases

    DMDMi74733576.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB035124 mRNA. Translation: BAB18635.1 .
    AK290746 mRNA. Translation: BAF83435.1 .
    CH471137 Genomic DNA. Translation: EAW51304.1 .
    BC015355 mRNA. Translation: AAH15355.1 .
    BC012024 mRNA. Translation: AAH12024.1 .
    CCDSi CCDS3998.1.
    RefSeqi NP_075060.1. NM_022909.3.
    UniGenei Hs.631967.

    3D structure databases

    ProteinModelPortali Q9H3R5.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 122355. 17 interactions.
    IntActi Q9H3R5. 1 interaction.
    MINTi MINT-4988967.
    STRINGi 9606.ENSP00000283006.

    PTM databases

    PhosphoSitei Q9H3R5.

    Polymorphism databases

    DMDMi 74733576.

    Proteomic databases

    MaxQBi Q9H3R5.
    PaxDbi Q9H3R5.
    PRIDEi Q9H3R5.

    Protocols and materials databases

    DNASUi 64946.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000283006 ; ENSP00000283006 ; ENSG00000153044 .
    GeneIDi 64946.
    KEGGi hsa:64946.
    UCSCi uc003jvp.3. human.

    Organism-specific databases

    CTDi 64946.
    GeneCardsi GC05P068496.
    HGNCi HGNC:17268. CENPH.
    HPAi HPA036494.
    MIMi 605607. gene.
    neXtProti NX_Q9H3R5.
    PharmGKBi PA26402.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG10470.
    HOGENOMi HOG000013068.
    HOVERGENi HBG081083.
    InParanoidi Q9H3R5.
    KOi K11500.
    OMAi QMEIKIT.
    OrthoDBi EOG7JQBRQ.
    PhylomeDBi Q9H3R5.
    TreeFami TF101134.

    Enzyme and pathway databases

    Reactomei REACT_150425. Resolution of Sister Chromatid Cohesion.
    REACT_150471. Separation of Sister Chromatids.
    REACT_22186. Deposition of new CENPA-containing nucleosomes at the centromere.
    REACT_682. Mitotic Prometaphase.

    Miscellaneous databases

    ChiTaRSi CENPH. human.
    GeneWikii CENPH.
    GenomeRNAii 64946.
    NextBioi 67122.
    PROi Q9H3R5.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q9H3R5.
    Bgeei Q9H3R5.
    CleanExi HS_CENPH.
    Genevestigatori Q9H3R5.

    Family and domain databases

    InterProi IPR008426. CENP-H.
    [Graphical view ]
    Pfami PF05837. CENP-H. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Human CENP-H multimers colocalize with CENP-A and CENP-C at active centromere-kinetochore complexes."
      Sugata N., Li S., Earnshaw W.C., Yen T.J., Yoda K., Masumoto H., Munekata E., Warburton P.E., Todokoro K.
      Hum. Mol. Genet. 9:2919-2926(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH KIF2C, SUBCELLULAR LOCATION.
      Tissue: Cervix carcinoma.
    2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: PlacentaImported and TestisImported.
    5. Cited for: FUNCTION.
    6. "The functional region of CENP-H interacts with the Nuf2 complex that localizes to centromere during mitosis."
      Mikami Y., Hori T., Kimura H., Fukagawa T.
      Mol. Cell. Biol. 25:1958-1970(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH NDC80.
    7. Cited for: FUNCTION.
    8. "Comprehensive analysis of the ICEN (Interphase Centromere Complex) components enriched in the CENP-A chromatin of human cells."
      Izuta H., Ikeno M., Suzuki N., Tomonaga T., Nozaki N., Obuse C., Kisu Y., Goshima N., Nomura F., Nomura N., Yoda K.
      Genes Cells 11:673-684(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY.
    9. "The CENP-H-I complex is required for the efficient incorporation of newly synthesized CENP-A into centromeres."
      Okada M., Cheeseman I.M., Hori T., Okawa K., McLeod I.X., Yates J.R. III, Desai A., Fukagawa T.
      Nat. Cell Biol. 8:446-457(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN A COMPLEX WITH CENPI; CENPK; CENPN; CENPO; CENPP; CENPQ; CENPR AND CENPU.
    10. Cited for: IDENTIFICATION IN THE CENPA-NAC COMPLEX WITH CENPA; CENPC; CENPM; CENPN; CENPT AND CENPU.
    11. "The CENP-A NAC/CAD kinetochore complex controls chromosome congression and spindle bipolarity."
      McClelland S.E., Borusu S., Amaro A.C., Winter J.R., Belwal M., McAinsh A.D., Meraldi P.
      EMBO J. 26:5033-5047(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION.
    12. "Co-localization of CENP-C and CENP-H to discontinuous domains of CENP-A chromatin at human neocentromeres."
      Alonso A., Fritz B., Hasson D., Abrusan G., Cheung F., Yoda K., Radlwimmer B., Ladurner A.G., Warburton P.E.
      Genome Biol. 8:R148.1-R148.19(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.
    13. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
      Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
      Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    14. "CENP-K and CENP-H may form coiled-coils in the kinetochores."
      Qiu S., Wang J., Yu C., He D.
      Sci. China, Ser. C, Life Sci. 52:352-359(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH CENPK.
    15. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    16. Cited for: VARIANT [LARGE SCALE ANALYSIS] LYS-2.

    Entry informationi

    Entry nameiCENPH_HUMAN
    AccessioniPrimary (citable) accession number: Q9H3R5
    Secondary accession number(s): A8K3Y1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 8, 2005
    Last sequence update: March 1, 2001
    Last modified: October 1, 2014
    This is version 109 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 5
      Human chromosome 5: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3