Q9H3R5 (CENPH_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Centromere protein H
Short name=CENP-H

Alternative name(s):
Interphase centromere complex protein 35

Gene names

Name:	CENPH
Synonyms:	ICEN35

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	247 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres. Required for chromosome congression and efficiently align the chromosomes on a metaphase plate. Ref.5 Ref.7 Ref.11
Subunit structure	Self-associates. Component of the CENPA-NAC complex, at least composed of CENPA, CENPC, CENPH, CENPM, CENPN, CENPT and MLF1IP/CENPU. The CENPA-NAC complex interacts with the CENPA-CAD complex, composed of CENPI, CENPK, CENPL, CENPO, CENPP, CENPQ, CENPR and CENPS. Interacts directly with CENPK. Interacts with KIF2C and NDC80. Interacts with TRIM36 By similarity. Ref.1 Ref.6 Ref.9 Ref.10 Ref.13
Subcellular location	Nucleus. Chromosome › centromere › kinetochore. Note: Associates with active centromere-kinetochore complexes throughout the cell cycle. Colocalizes with inner kinetochore plate proteins CENPA and CENPC1 during both interphase and metaphase. Ref.1 Ref.11 Ref.12
Sequence similarities	Belongs to the centromere protein H family.

Ontologies

Keywords
Cellular component	Centromere Chromosome Kinetochore Nucleus
Coding sequence diversity	Polymorphism
Domain	Coiled coil
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	CENP-A containing nucleosome assembly at centromere Traceable author statement. Source: Reactome cell division Inferred from electronic annotation. Source: InterPro chromosome segregation Inferred from electronic annotation. Source: InterPro kinetochore organization Traceable author statement Ref.1. Source: UniProtKB mitotic prometaphase Traceable author statement. Source: Reactome
Cellular_component	condensed chromosome kinetochore Inferred from electronic annotation. Source: UniProtKB-SubCell cytosol Traceable author statement. Source: Reactome kinetochore Inferred from mutant phenotype PubMed 20212317. Source: UniProtKB nucleoplasm Traceable author statement. Source: Reactome
Molecular_function	kinetochore binding Inferred from direct assay Ref.1. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
NDC80	O14777	3	EBI-1003700,EBI-715849

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 247	247	Centromere protein H		PRO_0000089478
Regions
	Coiled coil	47 – 192	146	Potential
Natural variations
	Natural variant	2	1	E → K in a colorectal cancer sample; somatic mutation. Ref.14		VAR_036167

Sequences

	Sequence		Length	Mass (Da)
	Q9H3R5 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: E1BCD4022710168F	FASTA	247	28,481
	10 20 30 40 50 60 MEEQPQMQDA DEPADSGGEG RAGGPPQVAG AQAACSEDRM TLLLRLRAQT KQQLLEYKSM 70 80 90 100 110 120 VDASEEKTPE QIMQEKQIEA KIEDLENEIE EVKVAFEIKK LALDRMRLST ALKKNLEKIS 130 140 150 160 170 180 RQSSVLMDNM KHLLELNKLI MKSQQESWDL EEKLLDIRKK RLQLKQASES KLLEIQTEKN 190 200 210 220 230 240 KQKIDLDSME NSERIKIIRQ NLQMEIKITT VIQHVFQNLI LGSKVNWAED PALKEIVLQL EKNVDMM

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Human CENP-H multimers colocalize with CENP-A and CENP-C at active centromere-kinetochore complexes." Sugata N., Li S., Earnshaw W.C., Yen T.J., Yoda K., Masumoto H., Munekata E., Warburton P.E., Todokoro K. Hum. Mol. Genet. 9:2919-2926(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH KIF2C, SUBCELLULAR LOCATION. Tissue: Cervix carcinoma.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Placenta and Testis.
[5]	"Transcription within a functional human centromere." Saffery R., Sumer H., Hassan S., Wong L.H., Craig J.M., Todokoro K., Anderson M., Stafford A., Choo K.H.A. Mol. Cell 12:509-516(2003) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[6]	"The functional region of CENP-H interacts with the Nuf2 complex that localizes to centromere during mitosis." Mikami Y., Hori T., Kimura H., Fukagawa T. Mol. Cell. Biol. 25:1958-1970(2005) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH NDC80.
[7]	"RNAi knockdown of human kinetochore protein CENP-H." Orthaus S., Ohndorf S., Diekmann S. Biochem. Biophys. Res. Commun. 348:36-46(2006) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[8]	"Comprehensive analysis of the ICEN (Interphase Centromere Complex) components enriched in the CENP-A chromatin of human cells." Izuta H., Ikeno M., Suzuki N., Tomonaga T., Nozaki N., Obuse C., Kisu Y., Goshima N., Nomura F., Nomura N., Yoda K. Genes Cells 11:673-684(2006) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY.
[9]	"The CENP-H-I complex is required for the efficient incorporation of newly synthesized CENP-A into centromeres." Okada M., Cheeseman I.M., Hori T., Okawa K., McLeod I.X., Yates J.R. III, Desai A., Fukagawa T. Nat. Cell Biol. 8:446-457(2006) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN A COMPLEX WITH CENPI; CENPK; CENPN; CENPO; CENPP; CENPQ; CENPR AND CENPU.
[10]	"The human CENP-A centromeric nucleosome-associated complex." Foltz D.R., Jansen L.E.T., Black B.E., Bailey A.O., Yates J.R. III, Cleveland D.W. Nat. Cell Biol. 8:458-469(2006) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN THE CENPA-NAC COMPLEX WITH CENPA; CENPC; CENPM; CENPN; CENPT AND CENPU.
[11]	"The CENP-A NAC/CAD kinetochore complex controls chromosome congression and spindle bipolarity." McClelland S.E., Borusu S., Amaro A.C., Winter J.R., Belwal M., McAinsh A.D., Meraldi P. EMBO J. 26:5033-5047(2007) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[12]	"Co-localization of CENP-C and CENP-H to discontinuous domains of CENP-A chromatin at human neocentromeres." Alonso A., Fritz B., Hasson D., Abrusan G., Cheung F., Yoda K., Radlwimmer B., Ladurner A.G., Warburton P.E. Genome Biol. 8:R148.1-R148.19(2007) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION.
[13]	"CENP-K and CENP-H may form coiled-coils in the kinetochores." Qiu S., Wang J., Yu C., He D. Sci. China, Ser. C, Life Sci. 52:352-359(2009) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH CENPK.
[14]	"The consensus coding sequences of human breast and colorectal cancers." Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D., Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P., Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V. , Gazdar A.F., Hartigan J., Wu L., Liu C., Parmigiani G., Park B.H., Bachman K.E., Papadopoulos N., Vogelstein B., Kinzler K.W., Velculescu V.E. Science 314:268-274(2006) [PubMed] [Europe PMC] [Abstract] Cited for: VARIANT [LARGE SCALE ANALYSIS] LYS-2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB035124 mRNA. Translation: BAB18635.1. AK290746 mRNA. Translation: BAF83435.1. CH471137 Genomic DNA. Translation: EAW51304.1. BC015355 mRNA. Translation: AAH15355.1. BC012024 mRNA. Translation: AAH12024.1.
IPI	IPI00009668.
RefSeq	NP_075060.1. NM_022909.3.
UniGene	Hs.631967.
3D structure databases
ProteinModelPortal	Q9H3R5.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9H3R5. 1 interaction.
STRING	9606.ENSP00000283006.
PTM databases
PhosphoSite	Q9H3R5.
Polymorphism databases
DMDM	74733576.
Proteomic databases
PaxDb	Q9H3R5.
PRIDE	Q9H3R5.
Protocols and materials databases
DNASU	64946.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000283006; ENSP00000283006; ENSG00000153044.
GeneID	64946.
KEGG	hsa:64946.
UCSC	uc003jvp.3. human.
Organism-specific databases
CTD	64946.
GeneCards	GC05P068486.
HGNC	HGNC:17268. CENPH.
HPA	HPA036494.
MIM	605607. gene.
neXtProt	NX_Q9H3R5.
PharmGKB	PA26402.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG10470.
HOGENOM	HOG000013068.
HOVERGEN	HBG081083.
InParanoid	Q9H3R5.
KO	K11500.
OMA	LMDNMKH.
OrthoDB	EOG4TF0M5.
PhylomeDB	Q9H3R5.
Enzyme and pathway databases
Reactome	REACT_115566. Cell Cycle. REACT_21300. Mitotic M-M/G1 phases.
Gene expression databases
ArrayExpress	Q9H3R5.
Bgee	Q9H3R5.
CleanEx	HS_CENPH.
Genevestigator	Q9H3R5.
GermOnline	ENSG00000153044. Homo sapiens.
Family and domain databases
InterPro	IPR008426. Centromere_CenpH. [Graphical view]
Pfam	PF05837. CENP-H. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	CENPH. human.
GenomeRNAi	64946.
NextBio	67122.
SOURCE	Search...

Entry information

Entry name

CENPH_HUMAN

Accession

Primary (citable) accession number: Q9H3R5
Secondary accession number(s): A8K3Y1

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 8, 2005
Last sequence update:	March 1, 2001
Last modified:	April 3, 2013
This is version 95 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.