Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q9H3R5 (CENPH_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 104. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Centromere protein H

Short name=CENP-H
Alternative name(s):
Interphase centromere complex protein 35
Gene names
Name:CENPH
Synonyms:ICEN35
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length247 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Component of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres. Required for chromosome congression and efficiently align the chromosomes on a metaphase plate. Ref.5 Ref.7 Ref.11

Subunit structure

Self-associates. Component of the CENPA-NAC complex, at least composed of CENPA, CENPC, CENPH, CENPM, CENPN, CENPT and CENPU. The CENPA-NAC complex interacts with the CENPA-CAD complex, composed of CENPI, CENPK, CENPL, CENPO, CENPP, CENPQ, CENPR and CENPS. Interacts directly with CENPK. Interacts with KIF2C and NDC80. Interacts with TRIM36 By similarity. Ref.1 Ref.6 Ref.9 Ref.10 Ref.14

Subcellular location

Nucleus. Chromosomecentromerekinetochore. Note: Associates with active centromere-kinetochore complexes throughout the cell cycle. Colocalizes with inner kinetochore plate proteins CENPA and CENPC during both interphase and metaphase. Ref.1 Ref.11 Ref.12

Sequence similarities

Belongs to the centromere protein H family.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

NDC80O147773EBI-1003700,EBI-715849

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 247247Centromere protein H
PRO_0000089478

Regions

Coiled coil47 – 192146 Potential

Amino acid modifications

Modified residue11N-acetylmethionine Ref.13 Ref.15

Natural variations

Natural variant21E → K in a colorectal cancer sample; somatic mutation. Ref.16
VAR_036167

Sequences

Sequence LengthMass (Da)Tools
Q9H3R5 [UniParc].

Last modified March 1, 2001. Version 1.
Checksum: E1BCD4022710168F

FASTA24728,481
        10         20         30         40         50         60 
MEEQPQMQDA DEPADSGGEG RAGGPPQVAG AQAACSEDRM TLLLRLRAQT KQQLLEYKSM 

        70         80         90        100        110        120 
VDASEEKTPE QIMQEKQIEA KIEDLENEIE EVKVAFEIKK LALDRMRLST ALKKNLEKIS 

       130        140        150        160        170        180 
RQSSVLMDNM KHLLELNKLI MKSQQESWDL EEKLLDIRKK RLQLKQASES KLLEIQTEKN 

       190        200        210        220        230        240 
KQKIDLDSME NSERIKIIRQ NLQMEIKITT VIQHVFQNLI LGSKVNWAED PALKEIVLQL 


EKNVDMM 

« Hide

References

« Hide 'large scale' references
[1]"Human CENP-H multimers colocalize with CENP-A and CENP-C at active centromere-kinetochore complexes."
Sugata N., Li S., Earnshaw W.C., Yen T.J., Yoda K., Masumoto H., Munekata E., Warburton P.E., Todokoro K.
Hum. Mol. Genet. 9:2919-2926(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH KIF2C, SUBCELLULAR LOCATION.
Tissue: Cervix carcinoma.
[2]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Placenta and Testis.
[5]"Transcription within a functional human centromere."
Saffery R., Sumer H., Hassan S., Wong L.H., Craig J.M., Todokoro K., Anderson M., Stafford A., Choo K.H.A.
Mol. Cell 12:509-516(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[6]"The functional region of CENP-H interacts with the Nuf2 complex that localizes to centromere during mitosis."
Mikami Y., Hori T., Kimura H., Fukagawa T.
Mol. Cell. Biol. 25:1958-1970(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH NDC80.
[7]"RNAi knockdown of human kinetochore protein CENP-H."
Orthaus S., Ohndorf S., Diekmann S.
Biochem. Biophys. Res. Commun. 348:36-46(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[8]"Comprehensive analysis of the ICEN (Interphase Centromere Complex) components enriched in the CENP-A chromatin of human cells."
Izuta H., Ikeno M., Suzuki N., Tomonaga T., Nozaki N., Obuse C., Kisu Y., Goshima N., Nomura F., Nomura N., Yoda K.
Genes Cells 11:673-684(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY.
[9]"The CENP-H-I complex is required for the efficient incorporation of newly synthesized CENP-A into centromeres."
Okada M., Cheeseman I.M., Hori T., Okawa K., McLeod I.X., Yates J.R. III, Desai A., Fukagawa T.
Nat. Cell Biol. 8:446-457(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN A COMPLEX WITH CENPI; CENPK; CENPN; CENPO; CENPP; CENPQ; CENPR AND CENPU.
[10]"The human CENP-A centromeric nucleosome-associated complex."
Foltz D.R., Jansen L.E.T., Black B.E., Bailey A.O., Yates J.R. III, Cleveland D.W.
Nat. Cell Biol. 8:458-469(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN THE CENPA-NAC COMPLEX WITH CENPA; CENPC; CENPM; CENPN; CENPT AND CENPU.
[11]"The CENP-A NAC/CAD kinetochore complex controls chromosome congression and spindle bipolarity."
McClelland S.E., Borusu S., Amaro A.C., Winter J.R., Belwal M., McAinsh A.D., Meraldi P.
EMBO J. 26:5033-5047(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION.
[12]"Co-localization of CENP-C and CENP-H to discontinuous domains of CENP-A chromatin at human neocentromeres."
Alonso A., Fritz B., Hasson D., Abrusan G., Cheung F., Yoda K., Radlwimmer B., Ladurner A.G., Warburton P.E.
Genome Biol. 8:R148.1-R148.19(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
[13]"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[14]"CENP-K and CENP-H may form coiled-coils in the kinetochores."
Qiu S., Wang J., Yu C., He D.
Sci. China, Ser. C, Life Sci. 52:352-359(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH CENPK.
[15]"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A., Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[16]"The consensus coding sequences of human breast and colorectal cancers."
Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D., Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P., Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V. expand/collapse author list , Gazdar A.F., Hartigan J., Wu L., Liu C., Parmigiani G., Park B.H., Bachman K.E., Papadopoulos N., Vogelstein B., Kinzler K.W., Velculescu V.E.
Science 314:268-274(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: VARIANT [LARGE SCALE ANALYSIS] LYS-2.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB035124 mRNA. Translation: BAB18635.1.
AK290746 mRNA. Translation: BAF83435.1.
CH471137 Genomic DNA. Translation: EAW51304.1.
BC015355 mRNA. Translation: AAH15355.1.
BC012024 mRNA. Translation: AAH12024.1.
RefSeqNP_075060.1. NM_022909.3.
UniGeneHs.631967.

3D structure databases

ProteinModelPortalQ9H3R5.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid122355. 17 interactions.
IntActQ9H3R5. 1 interaction.
MINTMINT-4988967.
STRING9606.ENSP00000283006.

PTM databases

PhosphoSiteQ9H3R5.

Polymorphism databases

DMDM74733576.

Proteomic databases

PaxDbQ9H3R5.
PRIDEQ9H3R5.

Protocols and materials databases

DNASU64946.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000283006; ENSP00000283006; ENSG00000153044.
GeneID64946.
KEGGhsa:64946.
UCSCuc003jvp.3. human.

Organism-specific databases

CTD64946.
GeneCardsGC05P068496.
HGNCHGNC:17268. CENPH.
HPAHPA036494.
MIM605607. gene.
neXtProtNX_Q9H3R5.
PharmGKBPA26402.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG10470.
HOGENOMHOG000013068.
HOVERGENHBG081083.
InParanoidQ9H3R5.
KOK11500.
OMALMDNMKH.
OrthoDBEOG7JQBRQ.
PhylomeDBQ9H3R5.
TreeFamTF101134.

Enzyme and pathway databases

ReactomeREACT_115566. Cell Cycle.
REACT_21300. Mitotic M-M/G1 phases.

Gene expression databases

ArrayExpressQ9H3R5.
BgeeQ9H3R5.
CleanExHS_CENPH.
GenevestigatorQ9H3R5.

Family and domain databases

InterProIPR008426. CENP-H.
[Graphical view]
PfamPF05837. CENP-H. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSCENPH. human.
GeneWikiCENPH.
GenomeRNAi64946.
NextBio67122.
PROQ9H3R5.
SOURCESearch...

Entry information

Entry nameCENPH_HUMAN
AccessionPrimary (citable) accession number: Q9H3R5
Secondary accession number(s): A8K3Y1
Entry history
Integrated into UniProtKB/Swiss-Prot: November 8, 2005
Last sequence update: March 1, 2001
Last modified: April 16, 2014
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 5

Human chromosome 5: entries, gene names and cross-references to MIM