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Protein

Centromere protein H

Gene

CENPH

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres. Required for chromosome congression and efficiently align the chromosomes on a metaphase plate.3 Publications

GO - Molecular functioni

  • kinetochore binding Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Enzyme and pathway databases

ReactomeiREACT_150425. Resolution of Sister Chromatid Cohesion.
REACT_150471. Separation of Sister Chromatids.
REACT_22186. Deposition of new CENPA-containing nucleosomes at the centromere.
REACT_355252. RHO GTPases Activate Formins.
REACT_682. Mitotic Prometaphase.

Names & Taxonomyi

Protein namesi
Recommended name:
Centromere protein H
Short name:
CENP-H
Alternative name(s):
Interphase centromere complex protein 35
Gene namesi
Name:CENPHImported
Synonyms:ICEN35
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 5

Organism-specific databases

HGNCiHGNC:17268. CENPH.

Subcellular locationi

  • Nucleus
  • Chromosomecentromerekinetochore

  • Note: Associates with active centromere-kinetochore complexes throughout the cell cycle. Colocalizes with inner kinetochore plate proteins CENPA and CENPC during both interphase and metaphase.

GO - Cellular componenti

  • condensed chromosome kinetochore Source: UniProtKB-SubCell
  • cytosol Source: Reactome
  • kinetochore Source: UniProtKB
  • nucleoplasm Source: Reactome
  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Centromere, Chromosome, Kinetochore, Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA26402.

Polymorphism and mutation databases

BioMutaiCENPH.
DMDMi74733576.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 247247Centromere protein HPRO_0000089478Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionine2 Publications

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiQ9H3R5.
PaxDbiQ9H3R5.
PRIDEiQ9H3R5.

PTM databases

PhosphoSiteiQ9H3R5.

Expressioni

Gene expression databases

BgeeiQ9H3R5.
CleanExiHS_CENPH.
ExpressionAtlasiQ9H3R5. baseline and differential.
GenevisibleiQ9H3R5. HS.

Organism-specific databases

HPAiHPA036494.

Interactioni

Subunit structurei

Self-associates. Component of the CENPA-NAC complex, at least composed of CENPA, CENPC, CENPH, CENPM, CENPN, CENPT and CENPU. The CENPA-NAC complex interacts with the CENPA-CAD complex, composed of CENPI, CENPK, CENPL, CENPO, CENPP, CENPQ, CENPR and CENPS. Interacts directly with CENPK. Interacts with KIF2C and NDC80. Interacts with TRIM36 (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
CENPKQ9BS163EBI-1003700,EBI-6871750
MTAPQ131263EBI-1003700,EBI-2547776
NDC80O147773EBI-1003700,EBI-715849

Protein-protein interaction databases

BioGridi122355. 26 interactions.
IntActiQ9H3R5. 4 interactions.
MINTiMINT-4988967.
STRINGi9606.ENSP00000283006.

Structurei

3D structure databases

ProteinModelPortaliQ9H3R5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili47 – 192146Sequence AnalysisAdd
BLAST

Sequence similaritiesi

Belongs to the centromere protein H family.Sequence Analysis

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiNOG10470.
GeneTreeiENSGT00390000009578.
HOGENOMiHOG000013068.
HOVERGENiHBG081083.
InParanoidiQ9H3R5.
KOiK11500.
OMAiQMEIKIT.
OrthoDBiEOG7JQBRQ.
PhylomeDBiQ9H3R5.
TreeFamiTF101134.

Family and domain databases

InterProiIPR008426. CENP-H.
[Graphical view]
PfamiPF05837. CENP-H. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9H3R5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEEQPQMQDA DEPADSGGEG RAGGPPQVAG AQAACSEDRM TLLLRLRAQT
60 70 80 90 100
KQQLLEYKSM VDASEEKTPE QIMQEKQIEA KIEDLENEIE EVKVAFEIKK
110 120 130 140 150
LALDRMRLST ALKKNLEKIS RQSSVLMDNM KHLLELNKLI MKSQQESWDL
160 170 180 190 200
EEKLLDIRKK RLQLKQASES KLLEIQTEKN KQKIDLDSME NSERIKIIRQ
210 220 230 240
NLQMEIKITT VIQHVFQNLI LGSKVNWAED PALKEIVLQL EKNVDMM
Length:247
Mass (Da):28,481
Last modified:March 1, 2001 - v1
Checksum:iE1BCD4022710168F
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti2 – 21E → K in a colorectal cancer sample; somatic mutation. 1 Publication
VAR_036167

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB035124 mRNA. Translation: BAB18635.1.
AK290746 mRNA. Translation: BAF83435.1.
CH471137 Genomic DNA. Translation: EAW51304.1.
BC015355 mRNA. Translation: AAH15355.1.
BC012024 mRNA. Translation: AAH12024.1.
CCDSiCCDS3998.1.
RefSeqiNP_075060.1. NM_022909.3.
UniGeneiHs.631967.

Genome annotation databases

EnsembliENST00000283006; ENSP00000283006; ENSG00000153044.
GeneIDi64946.
KEGGihsa:64946.
UCSCiuc003jvp.3. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB035124 mRNA. Translation: BAB18635.1.
AK290746 mRNA. Translation: BAF83435.1.
CH471137 Genomic DNA. Translation: EAW51304.1.
BC015355 mRNA. Translation: AAH15355.1.
BC012024 mRNA. Translation: AAH12024.1.
CCDSiCCDS3998.1.
RefSeqiNP_075060.1. NM_022909.3.
UniGeneiHs.631967.

3D structure databases

ProteinModelPortaliQ9H3R5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi122355. 26 interactions.
IntActiQ9H3R5. 4 interactions.
MINTiMINT-4988967.
STRINGi9606.ENSP00000283006.

PTM databases

PhosphoSiteiQ9H3R5.

Polymorphism and mutation databases

BioMutaiCENPH.
DMDMi74733576.

Proteomic databases

MaxQBiQ9H3R5.
PaxDbiQ9H3R5.
PRIDEiQ9H3R5.

Protocols and materials databases

DNASUi64946.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000283006; ENSP00000283006; ENSG00000153044.
GeneIDi64946.
KEGGihsa:64946.
UCSCiuc003jvp.3. human.

Organism-specific databases

CTDi64946.
GeneCardsiGC05P068508.
HGNCiHGNC:17268. CENPH.
HPAiHPA036494.
MIMi605607. gene.
neXtProtiNX_Q9H3R5.
PharmGKBiPA26402.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG10470.
GeneTreeiENSGT00390000009578.
HOGENOMiHOG000013068.
HOVERGENiHBG081083.
InParanoidiQ9H3R5.
KOiK11500.
OMAiQMEIKIT.
OrthoDBiEOG7JQBRQ.
PhylomeDBiQ9H3R5.
TreeFamiTF101134.

Enzyme and pathway databases

ReactomeiREACT_150425. Resolution of Sister Chromatid Cohesion.
REACT_150471. Separation of Sister Chromatids.
REACT_22186. Deposition of new CENPA-containing nucleosomes at the centromere.
REACT_355252. RHO GTPases Activate Formins.
REACT_682. Mitotic Prometaphase.

Miscellaneous databases

ChiTaRSiCENPH. human.
GeneWikiiCENPH.
GenomeRNAii64946.
NextBioi67122.
PROiQ9H3R5.
SOURCEiSearch...

Gene expression databases

BgeeiQ9H3R5.
CleanExiHS_CENPH.
ExpressionAtlasiQ9H3R5. baseline and differential.
GenevisibleiQ9H3R5. HS.

Family and domain databases

InterProiIPR008426. CENP-H.
[Graphical view]
PfamiPF05837. CENP-H. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Human CENP-H multimers colocalize with CENP-A and CENP-C at active centromere-kinetochore complexes."
    Sugata N., Li S., Earnshaw W.C., Yen T.J., Yoda K., Masumoto H., Munekata E., Warburton P.E., Todokoro K.
    Hum. Mol. Genet. 9:2919-2926(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH KIF2C, SUBCELLULAR LOCATION.
    Tissue: Cervix carcinoma.
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: PlacentaImported and TestisImported.
  5. Cited for: FUNCTION.
  6. "The functional region of CENP-H interacts with the Nuf2 complex that localizes to centromere during mitosis."
    Mikami Y., Hori T., Kimura H., Fukagawa T.
    Mol. Cell. Biol. 25:1958-1970(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH NDC80.
  7. Cited for: FUNCTION.
  8. "Comprehensive analysis of the ICEN (Interphase Centromere Complex) components enriched in the CENP-A chromatin of human cells."
    Izuta H., Ikeno M., Suzuki N., Tomonaga T., Nozaki N., Obuse C., Kisu Y., Goshima N., Nomura F., Nomura N., Yoda K.
    Genes Cells 11:673-684(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY.
  9. "The CENP-H-I complex is required for the efficient incorporation of newly synthesized CENP-A into centromeres."
    Okada M., Cheeseman I.M., Hori T., Okawa K., McLeod I.X., Yates J.R. III, Desai A., Fukagawa T.
    Nat. Cell Biol. 8:446-457(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN A COMPLEX WITH CENPI; CENPK; CENPN; CENPO; CENPP; CENPQ; CENPR AND CENPU.
  10. Cited for: IDENTIFICATION IN THE CENPA-NAC COMPLEX WITH CENPA; CENPC; CENPM; CENPN; CENPT AND CENPU.
  11. "The CENP-A NAC/CAD kinetochore complex controls chromosome congression and spindle bipolarity."
    McClelland S.E., Borusu S., Amaro A.C., Winter J.R., Belwal M., McAinsh A.D., Meraldi P.
    EMBO J. 26:5033-5047(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  12. "Co-localization of CENP-C and CENP-H to discontinuous domains of CENP-A chromatin at human neocentromeres."
    Alonso A., Fritz B., Hasson D., Abrusan G., Cheung F., Yoda K., Radlwimmer B., Ladurner A.G., Warburton P.E.
    Genome Biol. 8:R148.1-R148.19(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  13. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
    Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
    Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  14. "CENP-K and CENP-H may form coiled-coils in the kinetochores."
    Qiu S., Wang J., Yu C., He D.
    Sci. China, Ser. C, Life Sci. 52:352-359(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH CENPK.
  15. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  16. Cited for: VARIANT [LARGE SCALE ANALYSIS] LYS-2.

Entry informationi

Entry nameiCENPH_HUMAN
AccessioniPrimary (citable) accession number: Q9H3R5
Secondary accession number(s): A8K3Y1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 8, 2005
Last sequence update: March 1, 2001
Last modified: July 22, 2015
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 5
    Human chromosome 5: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.