Q9H3Q3 (G3ST2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Galactose-3-O-sulfotransferase 2
Short name=Gal3ST-2
EC=2.8.2.-

Alternative name(s):
Beta-galactose-3-O-sulfotransferase 2
Gal-beta-1, 3-GalNAc 3'-sulfotransferase 2
Glycoprotein beta-Gal 3'-sulfotransferase 2

Gene names

Name:	GAL3ST2
Synonyms:	GP3ST

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	398 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Transfers a sulfate group to the hydroxyl group at C3 of non-reducing beta-galactosyl residues. Acts both on type 1 (Gal-beta-1,3-GlcNAc) and type 2 (Gal-beta-1,4-GlcNAc) chains with similar efficiency.
Enzyme regulation	Strongly inhibited by Cu2+ and Zn2+. Ref.1
Pathway	Protein modification; carbohydrate sulfation.
Subcellular location	Golgi apparatus › Golgi stack membrane; Single-pass type II membrane protein Probable Ref.1.
Tissue specificity	Ubiquitous. Detected in heart, stomach, colon, liver and spleen, in epithelial cells lining the lower to middle layer of the crypts in colonic mucosa, hepatocytes surrounding the central vein of the liver, extravillous cytotrophoblasts in the basal plate of the septum of the placenta, renal tubules of the kidney, and neuronal cells of the cerebral cortex. Ref.1
Sequence similarities	Belongs to the galactose-3-O-sulfotransferase family.
Biophysicochemical properties	pH dependence: Optimum pH is 6.0-6.5.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Coding sequence diversity	Polymorphism
Domain	Signal-anchor Transmembrane Transmembrane helix
Molecular function	Transferase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular_component	Golgi cisterna membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW membrane Traceable author statement Ref.1. Source: UniProtKB
Molecular_function	galactosylceramide sulfotransferase activity Inferred from electronic annotation. Source: InterPro sulfotransferase activity Inferred from direct assay Ref.1. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 398	398	Galactose-3-O-sulfotransferase 2		PRO_0000085203
Regions
	Topological domain	1 – 10	10	Cytoplasmic Potential
	Transmembrane	11 – 31	21	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	32 – 398	367	Lumenal Potential
	Compositional bias	211 – 315	105	Arg-rich
Amino acid modifications
	Glycosylation	79	1	N-linked (GlcNAc...) Potential
	Glycosylation	132	1	N-linked (GlcNAc...) Potential
	Glycosylation	179	1	N-linked (GlcNAc...) Potential
	Glycosylation	287	1	N-linked (GlcNAc...) Potential
	Glycosylation	330	1	N-linked (GlcNAc...) Potential
	Glycosylation	360	1	N-linked (GlcNAc...) Potential
Natural variations
	Natural variant	4	1	M → L. Ref.1 Ref.4 Corresponds to variant rs12469459 [ dbSNP | Ensembl ].		VAR_047060

Sequences

	Sequence		Length	Mass (Da)
	Q9H3Q3 [UniParc]. Last modified November 4, 2008. Version 2. Checksum: 900FF26B515E82D5	FASTA	398	46,110
	10 20 30 40 50 60 MMSMLGGLQR YFRVILLLLL ALTLLLLAGF LHSDLELDTP LFGGQAEGPP VTNIMFLKTH 70 80 90 100 110 120 KTASSTVLNI LYRFAETHNL SVALPAGSRV HLGYPWLFLA RYVEGVGSQQ RFNIMCNHLR 130 140 150 160 170 180 FNLPQVQKVM PNDTFYFSIL RNPVFQLESS FIYYKTYAPA FRGAPSLDAF LASPRTFYND 190 200 210 220 230 240 SRHLRNVYAK NNMWFDFGFD PNAQCEEGYV RARIAEVERR FRLVLIAEHL DESLVLLRRR 250 260 270 280 290 300 LRWALDDVVA FRLNSRSARS VARLSPETRE RARSWCALDW RLYEHFNRTL WAQLRAELGP 310 320 330 340 350 360 RRLRGEVERL RARRRELASL CLQDGGALKN HTQIRDPRLR PYQSGKADIL GYNLRPGLDN 370 380 390 QTLGVCQRLV MPELQYMARL YALQFPEKPL KNIPFLGA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning and characterization of a human beta-Gal-3'-sulfotransferase that acts on both type 1 and type 2 (Galbeta 1-3/1-4GlcNAc-R) oligosaccharides." Honke K., Tsuda M., Koyota S., Wada Y., Iida-Tanaka N., Ishizuka I., Nakayama J., Taniguchi N. J. Biol. Chem. 276:267-274(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, TISSUE SPECIFICITY, ENZYME REGULATION, VARIANT LEU-4. Tissue: Colon.
[2]	"Generation and annotation of the DNA sequences of human chromosomes 2 and 4." Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K. Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT LEU-4.
+	Additional computationally mapped references.

Web resources

GGDB

GlycoGene database

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB040610 mRNA. Translation: BAB16844.1. AC114730 Genomic DNA. Translation: AAX82021.1. CH471063 Genomic DNA. Translation: EAW71293.1. BC117293 mRNA. Translation: AAI17294.1. BC117295 mRNA. Translation: AAI17296.1.
IPI	IPI00301475.
RefSeq	NP_071417.2. NM_022134.2.
UniGene	Hs.632556.
3D structure databases
ProteinModelPortal	Q9H3Q3.
ModBase	Search...
PTM databases
PhosphoSite	Q9H3Q3.
Polymorphism databases
DMDM	212276434.
Proteomic databases
PaxDb	Q9H3Q3.
PRIDE	Q9H3Q3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000192314; ENSP00000192314; ENSG00000154252.
GeneID	64090.
KEGG	hsa:64090.
UCSC	uc002wcj.1. human.
Organism-specific databases
CTD	64090.
GeneCards	GC02P242716.
HGNC	HGNC:24869. GAL3ST2.
MIM	608237. gene.
neXtProt	NX_Q9H3Q3.
PharmGKB	PA134913637.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG26344.
HOGENOM	HOG000232148.
HOVERGEN	HBG051681.
InParanoid	Q9H3Q3.
KO	K09675.
OMA	KRWCALD.
OrthoDB	EOG4W6NWD.
Enzyme and pathway databases
UniPathway	UPA00353.
Gene expression databases
Bgee	Q9H3Q3.
CleanEx	HS_GAL3ST2.
Genevestigator	Q9H3Q3.
GermOnline	ENSG00000154252. Homo sapiens.
Family and domain databases
InterPro	IPR009729. Gal-3-0_sulfotransfrase. [Graphical view]
PANTHER	PTHR14647. PTHR14647. 1 hit.
Pfam	PF06990. Gal-3-0_sulfotr. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	64090.
NextBio	65896.
SOURCE	Search...

Entry information

Entry name

G3ST2_HUMAN

Accession

Primary (citable) accession number: Q9H3Q3
Secondary accession number(s): Q17RK0, Q57Z52

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2004
Last sequence update:	November 4, 2008
Last modified:	April 3, 2013
This is version 85 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.