Q9H3Q1 (BORG4_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cdc42 effector protein 4

Alternative name(s):
Binder of Rho GTPases 4

Gene names

Name:	CDC42EP4
Synonyms:	BORG4, CEP4

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	356 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Probably involved in the organization of the actin cytoskeleton. May act downstream of CDC42 to induce actin filament assembly leading to cell shape changes. Induces pseudopodia formation, when overexpressed in fibroblasts.
Subunit structure	Interacts with CDC42 and RHOQ, in a GTP-dependent manner By similarity.
Subcellular location	Endomembrane system; Peripheral membrane protein. Cytoplasm › cytoskeleton.
Tissue specificity	Not detected in any of the adult tissues tested. May be expressed only in fetal or embryonic tissues.
Sequence similarities	Belongs to the BORG/CEP family. Contains 1 CRIB domain.

Ontologies

Keywords
Biological process	Cell shape
Cellular component	Cytoplasm Cytoskeleton Membrane
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	Rho protein signal transduction Inferred from electronic annotation. Source: Compara positive regulation of pseudopodium assembly Inferred from direct assay Ref.2. Source: UniProtKB regulation of cell shape Inferred from direct assay Ref.2. Source: UniProtKB
Cellular_component	actin cytoskeleton Inferred from direct assay. Source: HPA cytoplasm Inferred from electronic annotation. Source: UniProtKB-KW endomembrane system Inferred from electronic annotation. Source: UniProtKB-SubCell membrane Inferred from electronic annotation. Source: UniProtKB-KW microtubule cytoskeleton Inferred from direct assay. Source: HPA
Molecular_function	GTP-Rho binding Non-traceable author statement Ref.2. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 356	356	Cdc42 effector protein 4		PRO_0000212655
Regions
	Domain	27 – 41	15	CRIB
	Compositional bias	70 – 74	5	Poly-Ser
Amino acid modifications
	Modified residue	64	1	Phosphoserine Ref.5 Ref.7
	Modified residue	109	1	Phosphoserine By similarity
	Modified residue	118	1	Phosphoserine Ref.7
	Modified residue	138	1	Phosphoserine Ref.5
	Modified residue	140	1	Phosphoserine Ref.7
	Modified residue	141	1	Phosphoserine By similarity
	Modified residue	142	1	Phosphoserine Ref.7
	Modified residue	174	1	Phosphoserine Ref.4 Ref.5 Ref.7
	Modified residue	292	1	Phosphoserine Ref.4 Ref.5
	Modified residue	295	1	Phosphoserine Ref.4 Ref.5 Ref.7
Experimental info
	Sequence conflict	2	1	P → L in AAH10451. Ref.3
	Sequence conflict	33	1	D → T in AAD16299. Ref.2
	Sequence conflict	288	1	A → T in AAD16299. Ref.2
	Sequence conflict	296	1	A → T in AAD16299. Ref.2
	Sequence conflict	339	1	P → PP in AAD16299. Ref.2
	Sequence conflict	355	1	Missing in AAD16299. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q9H3Q1 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: 2CF677C60C6EF1B5	FASTA	356	37,980
	10 20 30 40 50 60 MPILKQLVSS SVHSKRRSRA DLTAEMISAP LGDFRHTMHV GRAGDAFGDT SFLNSKAGEP 70 80 90 100 110 120 DGESLDEQPS SSSSKRSLLS RKFRGSKRSQ SVTRGEREQR DMLGSLRDSA LFVKNAMSLP 130 140 150 160 170 180 QLNEKEAAEK GTSKLPKSLS SSPVKKANDG EGGDEEAGTE EAVPRRNGAA GPHSPDPLLD 190 200 210 220 230 240 EQAFGDLTDL PVVPKATYGL KHAESIMSFH IDLGPSMLGD VLSIMDKEEW DPEEGEGGYH 250 260 270 280 290 300 GDEGAAGTIT QAPPYAVAAP PLARQEGKAG PDLPSLPSHA LEDEGWAAAA PSPGSARSMG 310 320 330 340 350 SHTTRDSSSL SSCTSGILEE RSPAFRGPDR ARAAVSRQPD KEFSFMDEEE EDEIRV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence analysis, gene expression, and chromosomal assignment of mouse Borg4 gene and its human orthologue." Osada N., Kusuda J., Suzuki Y., Sugano S., Hashimoto K. J. Hum. Genet. 45:374-377(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Ileal mucosa.
[2]	"A new family of Cdc42 effector proteins, CEPs, function in fibroblast and epithelial cell shape changes." Hirsch D.S., Pirone D.M., Burbelo P.D. J. Biol. Chem. 276:875-883(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION. Tissue: Eye.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain and Skin.
[4]	"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-174; SER-292 AND SER-295, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[5]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-64; SER-138; SER-174; SER-292 AND SER-295, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[6]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Leukemic T-cell.
[7]	"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis." Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M. Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-64; SER-118; SER-140; SER-142; SER-174 AND SER-295, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB042237 mRNA. Translation: BAB17272.1. AF099664 mRNA. Translation: AAD16299.1. BC002774 mRNA. Translation: AAH02774.1. BC010451 mRNA. Translation: AAH10451.1.
IPI	IPI00015894.
RefSeq	NP_036253.2. NM_012121.4.
UniGene	Hs.3903.
3D structure databases
ProteinModelPortal	Q9H3Q1.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9H3Q1. 3 interactions.
MINT	MINT-1464445.
STRING	9606.ENSP00000338258.
PTM databases
PhosphoSite	Q9H3Q1.
Polymorphism databases
DMDM	21362403.
Proteomic databases
PaxDb	Q9H3Q1.
PeptideAtlas	Q9H3Q1.
PRIDE	Q9H3Q1.
Protocols and materials databases
DNASU	23580.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000335793; ENSP00000338258; ENSG00000179604.
GeneID	23580.
KEGG	hsa:23580.
UCSC	uc002jjn.3. human.
Organism-specific databases
CTD	23580.
GeneCards	GC17M071279.
HGNC	HGNC:17147. CDC42EP4.
HPA	HPA023335. HPA024797.
MIM	605468. gene.
neXtProt	NX_Q9H3Q1.
PharmGKB	PA38439.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG44582.
HOGENOM	HOG000231054.
HOVERGEN	HBG052803.
InParanoid	Q9H3Q1.
OMA	IMDKEEW.
OrthoDB	EOG4WWRKP.
PhylomeDB	Q9H3Q1.
Gene expression databases
ArrayExpress	Q9H3Q1.
Bgee	Q9H3Q1.
CleanEx	HS_CDC42EP4.
Genevestigator	Q9H3Q1.
GermOnline	ENSG00000179604. Homo sapiens.
Family and domain databases
InterPro	IPR000095. PAK_box_Rho-bd. [Graphical view]
Pfam	PF00786. PBD. 1 hit. [Graphical view]
PROSITE	PS50108. CRIB. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	CDC42EP4. human.
GenomeRNAi	23580.
NextBio	46182.
SOURCE	Search...

Entry information

Entry name

BORG4_HUMAN

Accession

Primary (citable) accession number: Q9H3Q1
Secondary accession number(s): O95828, Q96FT3

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 6, 2002
Last sequence update:	March 1, 2001
Last modified:	April 3, 2013
This is version 102 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.