Reviewed,
UniProtKB/Swiss-Prot Q9H3P7 (GCP60_HUMAN)
Last modified
November 24, 2009.
Version 73.
History...
Clusters with 100%,
90%,
50% identity |
Documents (5) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Golgi resident protein GCP60 Alternative name(s): Acyl-CoA-binding domain-containing protein 3 Golgi phosphoprotein 1 Short name=GOLPH1 Golgi complex-associated protein 1 Short name=GOCAP1 PBR- and PKA-associated protein 7 Peripheral benzodiazepine receptor-associated protein PAP7 | ||||
| Gene names |
| ||||
| Organism | Homo sapiens (Human) [Complete proteome] | ||||
| Taxonomic identifier | 9606 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Protein attributes
| Sequence length | 528 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Involved in the maintenance of Golgi structure by interacting with giantin, affecting protein transport between the endoplasmic reticulum and Golgi. Involved in hormone-induced steroid biosynthesis in testicular Leydig cells By similarity. |
| Subunit structure | Interacts with the C-terminal cytoplasmic domain of giantin/GOLGB1. Interacts with PBR and PKA regulatory subunit RI-alpha. Does not interact with PKA regulatory subunit RI-beta nor PKA regulatory subunit RII-alpha By similarity. |
| Subcellular location | Golgi apparatus membrane; Peripheral membrane protein; Cytoplasmic side By similarity. Mitochondrion By similarity. Note: Also mitochondrial (via its interaction with PBR) By similarity. |
| Tissue specificity | Ubiquitous, with highest expression in testis and ovary. Ref.1 |
| Domain | The GOLD domain is essential for giantin binding. |
| Sequence similarities | Contains 1 ACB (acyl-CoA-binding) domain. Contains 1 GOLD domain. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Lipid synthesis Steroid biosynthesis |
| Cellular component | Golgi apparatus Membrane Mitochondrion |
| Coding sequence diversity | Polymorphism |
| Domain | Coiled coil |
| PTM | Phosphoprotein |
| Technical term | Complete proteome Direct protein sequencing |
| Gene Ontology (GO) | |
| Biological process | steroid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW |
| Cellular component | Golgi apparatus Inferred from direct assay. Source: HPA membraneInferred from electronic annotation. Source: UniProtKB-KW mitochondrionInferred from electronic annotation. Source: UniProtKB-SubCell |
| Molecular function | acyl-CoA binding Inferred from electronic annotation. Source: InterPro protein bindingInferred from physical interaction. Source: IntAct |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Initiator methionine | 1 | 1 | Removed Ref.7 | ||||||
| Chain | 2 – 528 | 527 | Golgi resident protein GCP60 | PRO_0000214029 | |||||
Regions | |||||||||
| Domain | 83 – 174 | 92 | ACB | ||||||
| Domain | 384 – 526 | 143 | GOLD | ||||||
| Coiled coil | 174 – 257 | 84 | Potential | ||||||
| Compositional bias | 21 – 60 | 40 | Pro-rich | ||||||
| Compositional bias | 182 – 240 | 59 | Glu-rich | ||||||
| Compositional bias | 196 – 238 | 43 | Arg-rich | ||||||
| Compositional bias | 241 – 308 | 68 | Gln-rich | ||||||
Amino acid modifications | |||||||||
| Modified residue | 43 | 1 | Phosphoserine Ref.8 | ||||||
Natural variations | |||||||||
| Natural variant | 187 | 1 | E → D: dbSNP rs2306120. Ref.2 Ref.3 | VAR_019615 | |||||
Experimental info | |||||||||
| Sequence conflict | 93 | 1 | F → L in AAN60219. Ref.2 | ||||||
| Sequence conflict | 230 | 1 | R → I in AAN60219. Ref.2 | ||||||
| Sequence conflict | 265 | 1 | Q → R in AAN60219. Ref.2 | ||||||
| Sequence conflict | 333 | 1 | S → P in AAN60219. Ref.2 | ||||||
| Sequence conflict | 384 – 397 | 14 | KEKIQ…SVITV → QREDSAGCRFRDYS Ref.2 | ||||||
| Sequence conflict | 425 | 1 | Y → C in AAN60219. Ref.2 | ||||||
| Sequence conflict | 473 | 1 | K → R in AAH60792. Ref.6 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Identification and characterization of a novel Golgi protein, GCP60, that interacts with the integral membrane protein giantin." Sohda M., Misumi Y., Yamamoto A., Yano A., Nakamura N., Ikehara Y. J. Biol. Chem. 276:45298-45306(2001) [PubMed: 11590181] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH GOLGB1, TISSUE SPECIFICITY. Tissue: Cervix carcinoma. |
| [2] | Liu J., Tobin D., Tasken K., Papadopoulos V. Submitted (SEP-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT ASP-187. Tissue: Placenta. |
| [3] | "Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. Sugano S.Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT ASP-187. Tissue: Trachea. |
| [4] | "The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. Bentley D.R.Nature 441:315-321(2006) [PubMed: 16710414] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. |
| [5] | Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. Venter J.C.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. |
| [6] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain and Ovary. |
| [7] | "Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides." Gevaert K., Goethals M., Martens L., Van Damme J., Staes A., Thomas G.R., Vandekerckhove J. Nat. Biotechnol. 21:566-569(2003) [PubMed: 12665801] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-9. Tissue: Platelet. |
| [8] | "Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle." Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M. Mol. Cell 31:438-448(2008) [PubMed: 18691976] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-43, MASS SPECTROMETRY. |
| [9] | Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| AB043587 mRNA. Translation: BAB20592.2. AY150218 mRNA. Translation: AAN60219.1. AK025520 mRNA. Translation: BAB15159.1. AK314468 mRNA. Translation: BAG37076.1. AL592045 Genomic DNA. Translation: CAH71922.1. CH471098 Genomic DNA. Translation: EAW69775.1. BC034563 mRNA. Translation: AAH34563.2. BC045533 mRNA. Translation: AAH45533.1. BC060792 mRNA. Translation: AAH60792.1. | |
| IPI | IPI00009315. |
| RefSeq | NP_073572.2. |
| UniGene | Hs.520207 |
3D structure databases | |
| ModBase | Search... |
Protein-protein interaction databases | |
| IntAct | Q9H3P7. 1 interaction. |
| STRING | Q9H3P7. |
PTM databases | |
| PhosphoSite | Q9H3P7. |
Proteomic databases | |
| PeptideAtlas | Q9H3P7. |
| PRIDE | Q9H3P7. |
Genome annotation databases | |
| Ensembl | ENST00000366812; ENSP00000355777; ENSG00000182827; Homo sapiens. [Genome view] |
| GeneID | 64746. |
| KEGG | hsa:64746. |
| UCSC | uc001hpy.1. human. |
Organism-specific databases | |
| CTD | 64746. |
| GeneCards | GC01M224399. |
| H-InvDB | HIX0001643. HIX0056987. |
| HGNC | HGNC:15453. ACBD3. |
| HPA | HPA015594. |
| MIM | 606809. gene. |
| PharmGKB | PA28803. |
| GenAtlas | Search... |
Phylogenomic databases | |
| HOGENOM | Q9H3P7. |
| HOVERGEN | Q9H3P7. |
| OMA | ENGPKDS |
| OrthoDB | EOG9030BK |
Gene expression databases | |
| ArrayExpress | Q9H3P7. |
| Bgee | Q9H3P7. |
| CleanEx | HS_ACBD3. |
| Genevestigator | Q9H3P7. |
| GermOnline | ENSG00000182827. Homo sapiens. |
Family and domain databases | |
| InterPro | IPR000582. Acyl-CoA-binding_protein. IPR014352. FERM/acyl-CoA_bd_prot_3-hlx. IPR009038. GOLD. [Graphical view] |
| Gene3D | G3DSA:1.20.80.10. ACBP. 1 hit. |
| Pfam | PF00887. ACBP. 1 hit. [Graphical view] |
| PROSITE | PS00880. ACB_1. 1 hit. PS51228. ACB_2. 1 hit. PS50866. GOLD. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 66697. |
| SOURCE | Search... |
Entry information
| Entry name | GCP60_HUMAN | ||||||||
| Accession | Primary (citable) accession number: Q9H3P7 Secondary accession number(s): B2RB29 Q9H6U3 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
| Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. | ||||||||
Relevant documents
| Human chromosome 1 Human chromosome 1: entries, gene names and cross-references to MIM |
| Human entries with polymorphisms or disease mutations List of human entries with polymorphisms or disease mutations |
| Human polymorphisms and disease mutations Index of human polymorphisms and disease mutations |
| MIM cross-references Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot |
| SIMILARITY comments Index of protein domains and families |

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