Reviewed, UniProtKB/Swiss-Prot Q9H3P7 (GCP60_HUMAN)

Names and origin

Protein names

Recommended name:
    Golgi resident protein GCP60
Alternative name(s):
    Acyl-CoA-binding domain-containing protein 3
    Golgi phosphoprotein 1
      Short name=GOLPH1
    Golgi complex-associated protein 1
      Short name=GOCAP1
    PBR- and PKA-associated protein 7
    Peripheral benzodiazepine receptor-associated protein PAP7

Gene names

Name:	ACBD3
Synonyms:	GCP60, GOCAP1, GOLPH1

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	528 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Involved in the maintenance of Golgi structure by interacting with giantin, affecting protein transport between the endoplasmic reticulum and Golgi. Involved in hormone-induced steroid biosynthesis in testicular Leydig cells By similarity.
Subunit structure	Interacts with the C-terminal cytoplasmic domain of giantin/GOLGB1. Interacts with PBR and PKA regulatory subunit RI-alpha. Does not interact with PKA regulatory subunit RI-beta nor PKA regulatory subunit RII-alpha By similarity.
Subcellular location	Golgi apparatus membrane; Peripheral membrane protein; Cytoplasmic side By similarity. Mitochondrion By similarity. Note: Also mitochondrial (via its interaction with PBR) By similarity.
Tissue specificity	Ubiquitous, with highest expression in testis and ovary. Ref.1
Domain	The GOLD domain is essential for giantin binding.
Sequence similarities	Contains 1 ACB (acyl-CoA-binding) domain. Contains 1 GOLD domain.

Ontologies

Keywords
Biological process	Lipid synthesis Steroid biosynthesis
Cellular component	Golgi apparatus Membrane Mitochondrion
Coding sequence diversity	Polymorphism
Domain	Coiled coil
PTM	Phosphoprotein
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	steroid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	Golgi apparatus Inferred from direct assay. Source: HPA membrane Inferred from electronic annotation. Source: UniProtKB-KW mitochondrion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	acyl-CoA binding Inferred from electronic annotation. Source: InterPro protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
GOLGA3	Q08378	1	EBI-1791792,EBI-355782

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.7
	Chain	2 – 528	527	Golgi resident protein GCP60		PRO_0000214029
Regions
	Domain	83 – 174	92	ACB
	Domain	384 – 526	143	GOLD
	Coiled coil	174 – 257	84	Potential
	Compositional bias	21 – 60	40	Pro-rich
	Compositional bias	182 – 240	59	Glu-rich
	Compositional bias	196 – 238	43	Arg-rich
	Compositional bias	241 – 308	68	Gln-rich
Amino acid modifications
	Modified residue	43	1	Phosphoserine Ref.8
Natural variations
	Natural variant	187	1	E → D: dbSNP rs2306120. Ref.2 Ref.3		VAR_019615
Experimental info
	Sequence conflict	93	1	F → L in AAN60219. Ref.2
	Sequence conflict	230	1	R → I in AAN60219. Ref.2
	Sequence conflict	265	1	Q → R in AAN60219. Ref.2
	Sequence conflict	333	1	S → P in AAN60219. Ref.2
	Sequence conflict	384 – 397	14	KEKIQ…SVITV → QREDSAGCRFRDYS Ref.2
	Sequence conflict	425	1	Y → C in AAN60219. Ref.2
	Sequence conflict	473	1	K → R in AAH60792. Ref.6

Sequences

	Sequence		Length	Mass (Da)
	Q9H3P7-1 [UniParc]. Last modified January 23, 2007. Version 4. Checksum: B36EC550F8268FC2	FASTA	528	60,593
	10 20 30 40 50 60 MAAVLNAERL EVSVDGLTLS PDPEERPGAE GAPLLPPPLP PPSPPGSGRG PGASGEQPEP 70 80 90 100 110 120 GEAAAGGAAE EARRLEQRWG FGLEELYGLA LRFFKEKDGK AFHPTYEEKL KLVALHKQVL 130 140 150 160 170 180 MGPYNPDTCP EVGFFDVLGN DRRREWAALG NMSKEDAMVE FVKLLNRCCH LFSTYVASHK 190 200 210 220 230 240 IEKEEQEKKR KEEEERRRRE EEERERLQKE EEKRRREEEE RLRREEEERR RIEEERLRLE 250 260 270 280 290 300 QQKQQIMAAL NSQTAVQFQQ YAAQQYPGNY EQQQILIRQL QEQHYQQYMQ QLYQVQLAQQ 310 320 330 340 350 360 QAALQKQQEV VVAGSSLPTS SKVNATVPSN MMSVNGQAKT HTDSSEKELE PEAAEEALEN 370 380 390 400 410 420 GPKESLPVIA APSMWTRPQI KDFKEKIQQD ADSVITVGRG EVVTVRVPTH EEGSYLFWEF 430 440 450 460 470 480 ATDNYDIGFG VYFEWTDSPN TAVSVHVSES SDDDEEEEEN IGCEEKAKKN ANKPLLDEIV 490 500 510 520 PVYRRDCHEE VYAGSHQYPG RGVYLLKFDN SYSLWRSKSV YYRVYYTR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification and characterization of a novel Golgi protein, GCP60, that interacts with the integral membrane protein giantin." Sohda M., Misumi Y., Yamamoto A., Yano A., Nakamura N., Ikehara Y. J. Biol. Chem. 276:45298-45306(2001) [PubMed: 11590181] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH GOLGB1, TISSUE SPECIFICITY. Tissue: Cervix carcinoma.
[2]	Liu J., Tobin D., Tasken K., Papadopoulos V. Submitted (SEP-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT ASP-187. Tissue: Placenta.
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT ASP-187. Tissue: Trachea.
[4]	"The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R. Nature 441:315-321(2006) [PubMed: 16710414] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain and Ovary.
[7]	"Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides." Gevaert K., Goethals M., Martens L., Van Damme J., Staes A., Thomas G.R., Vandekerckhove J. Nat. Biotechnol. 21:566-569(2003) [PubMed: 12665801] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-9. Tissue: Platelet.
[8]	"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle." Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M. Mol. Cell 31:438-448(2008) [PubMed: 18691976] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-43, MASS SPECTROMETRY.
[9]	Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB043587 mRNA. Translation: BAB20592.2. AY150218 mRNA. Translation: AAN60219.1. AK025520 mRNA. Translation: BAB15159.1. AK314468 mRNA. Translation: BAG37076.1. AL592045 Genomic DNA. Translation: CAH71922.1. CH471098 Genomic DNA. Translation: EAW69775.1. BC034563 mRNA. Translation: AAH34563.2. BC045533 mRNA. Translation: AAH45533.1. BC060792 mRNA. Translation: AAH60792.1.
IPI	IPI00009315.
RefSeq	NP_073572.2.
UniGene	Hs.520207
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9H3P7. 1 interaction.
STRING	Q9H3P7.
PTM databases
PhosphoSite	Q9H3P7.
Proteomic databases
PeptideAtlas	Q9H3P7.
PRIDE	Q9H3P7.
Genome annotation databases
Ensembl	ENST00000366812; ENSP00000355777; ENSG00000182827; Homo sapiens. [Genome view]
GeneID	64746.
KEGG	hsa:64746.
UCSC	uc001hpy.1. human.
Organism-specific databases
CTD	64746.
GeneCards	GC01M224399.
H-InvDB	HIX0001643. HIX0056987.
HGNC	HGNC:15453. ACBD3.
HPA	HPA015594.
MIM	606809. gene.
PharmGKB	PA28803.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	Q9H3P7.
HOVERGEN	Q9H3P7.
OMA	ENGPKDS
OrthoDB	EOG9030BK
Gene expression databases
ArrayExpress	Q9H3P7.
Bgee	Q9H3P7.
CleanEx	HS_ACBD3.
Genevestigator	Q9H3P7.
GermOnline	ENSG00000182827. Homo sapiens.
Family and domain databases
InterPro	IPR000582. Acyl-CoA-binding_protein. IPR014352. FERM/acyl-CoA_bd_prot_3-hlx. IPR009038. GOLD. [Graphical view]
Gene3D	G3DSA:1.20.80.10. ACBP. 1 hit.
Pfam	PF00887. ACBP. 1 hit. [Graphical view]
PROSITE	PS00880. ACB_1. 1 hit. PS51228. ACB_2. 1 hit. PS50866. GOLD. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	66697.
SOURCE	Search...

Entry information

Entry name

GCP60_HUMAN

Accession

Primary (citable) accession number: Q9H3P7
Secondary accession number(s): B2RB29 , Q5VTJ0, Q6P9F1, Q8IZC5, Q8N4D6, Q9H6U3

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2004
Last sequence update:	January 23, 2007
Last modified:	November 24, 2009
This is version 73 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.