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Protein

Golgi resident protein GCP60

Gene

ACBD3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the maintenance of Golgi structure by interacting with giantin, affecting protein transport between the endoplasmic reticulum and Golgi (PubMed:11590181). Involved in hormone-induced steroid biosynthesis in testicular Leydig cells (By similarity). Recruits PI4KB to the Golgi apparatus membrane; enhances the enzyme activity of PI4KB activity via its membrane recruitment thereby increasing the local concentration of the substrate in the vicinity of the kinase (PubMed:27009356).By similarity2 Publications
(Microbial infection) Sensitizes PI4KB activation by Kobuviral (Aichi) 3A protein.1 Publication

GO - Molecular functioni

  • fatty-acyl-CoA binding Source: InterPro
  • protein kinase A regulatory subunit binding Source: UniProtKB

GO - Biological processi

Keywordsi

Biological processLipid biosynthesis, Lipid metabolism, Steroid biosynthesis

Enzyme and pathway databases

ReactomeiR-HSA-432722. Golgi Associated Vesicle Biogenesis.

Names & Taxonomyi

Protein namesi
Recommended name:
Golgi resident protein GCP60
Alternative name(s):
Acyl-CoA-binding domain-containing protein 3
Golgi complex-associated protein 1
Short name:
GOCAP1
Golgi phosphoprotein 1
Short name:
GOLPH1
PBR- and PKA-associated protein 7
Peripheral benzodiazepine receptor-associated protein PAP7
Cleaved into the following chain:
Gene namesi
Name:ACBD3
Synonyms:GCP60, GOCAP1, GOLPH1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

EuPathDBiHostDB:ENSG00000182827.8.
HGNCiHGNC:15453. ACBD3.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Golgi apparatus, Membrane, Mitochondrion

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi243K → A: No effect on PI4KB- and TBC1D22B-binding. 1 Publication1
Mutagenesisi244Q → A: No effect on PI4KB- and TBC1D22B-binding. 1 Publication1
Mutagenesisi245Q → A: No effect on PI4KB- and TBC1D22B-binding. 1 Publication1
Mutagenesisi246I → A: Partial loss of PI4KB- and TBC1D22B-binding. 1 Publication1
Mutagenesisi253Q → A: No effect on PI4KB- and TBC1D22B-binding. 1 Publication1
Mutagenesisi254T → A: No effect on PI4KB- and TBC1D22B-binding. 1 Publication1
Mutagenesisi256 – 258VQF → AAA: Loss of PI4KB-, TBC1D22A- and TBC1D22B-binding. 1 Publication3
Mutagenesisi257Q → A: Partial loss of PI4KB- and TBC1D22B-binding. 1 Publication1
Mutagenesisi258 – 259FQ → AA: Loss of interaction with PI4KB. 1 Publication2
Mutagenesisi258F → A: Differential effect on PI4KB- and TBC1D22B-binding, with PI4KB-binding being much more affected than TBC1D22B-binding. 2 Publications1
Mutagenesisi259Q → A: No effect on PI4KB- and TBC1D22B-binding. 1 Publication1
Mutagenesisi260Q → A: No effect on PI4KB- and TBC1D22B-binding. 1 Publication1
Mutagenesisi261Y → A: No effect on PI4KB- and TBC1D22B-binding. 1 Publication1
Mutagenesisi264 – 266QQY → AAA: No effect on PI4KB-, TBC1D22A- and TBC1D22B-binding. 1 Publication3
Mutagenesisi266Y → A: No loss of interaction with PI4KB. 1 Publication1
Mutagenesisi267 – 269PGN → AAA: Loss of PI4KB-, TBC1D22A- and TBC1D22B-binding. 1 Publication3
Mutagenesisi275I → A: No effect on PI4KB- and TBC1D22B-binding. 1 Publication1
Mutagenesisi276L → A: No effect on PI4KB- and TBC1D22B-binding. 1 Publication1
Mutagenesisi282 – 285EQHY → AAAA: Loss of PI4KB-, TBC1D22A- and TBC1D22B-binding. 1 Publication4
Mutagenesisi284H → A: Almost complete loss of PI4KB- and TBC1D22B-binding. 2 Publications1
Mutagenesisi285Y → A: Differential loss of PI4KB- and TBC1D22B-binding, with PI4KB-binding being much more affected than TBC1D22B-binding. 2 Publications1
Mutagenesisi286Q → A: No effect on PI4KB- and TBC1D22B-binding. 1 Publication1
Mutagenesisi287Q → A: No effect on PI4KB- and TBC1D22B-binding. 1 Publication1
Mutagenesisi288Y → A: Almost complete loss of PI4KB- and TBC1D22B-binding. 2 Publications1
Mutagenesisi344 – 345SS → AA: No effect on PI4KB-, TBC1D22A- and TBC1D22B-binding. 1 Publication2
Mutagenesisi380 – 381IK → AE: No effect on interaction with PI4KB but loss of interaction with Kobuviral (Aichi) 3A protein. Loss of ability to sensitize PI4KB activation by Kobuviral (Aichi) 3A protein. 1 Publication2
Mutagenesisi414 – 416SYL → AAA: No effect on PI4KB-, TBC1D22A- and TBC1D22B-binding. 1 Publication3
Mutagenesisi417 – 420FWEF → AAAA: No effect on PI4KB-, TBC1D22A- and TBC1D22B-binding. 1 Publication4
Mutagenesisi433 – 435FEW → AAA: No effect on PI4KB-, TBC1D22A- and TBC1D22B-binding. 1 Publication3
Mutagenesisi494 – 496GSH → AAA: No effect on PI4KB-, TBC1D22A- and TBC1D22B-binding. 1 Publication3
Mutagenesisi511 – 513SYS → AAA: No effect on PI4KB-, TBC1D22A- and TBC1D22B-binding. 1 Publication3
Mutagenesisi511S → A: Partial loss of PI4KB- and TBC1D22B-binding. 1 Publication1
Mutagenesisi525Y → A: No effect on interaction with PI4KB but loss of interaction with Kobuviral (Aichi) 3A protein. Loss of ability to sensitize PI4KB activation by Kobuviral (Aichi) 3A protein. 1 Publication1

Organism-specific databases

DisGeNETi64746.
OpenTargetsiENSG00000182827.
PharmGKBiPA28803.

Polymorphism and mutation databases

BioMutaiACBD3.
DMDMi51316096.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004364491 – 528Golgi resident protein GCP60Add BLAST528
Initiator methionineiRemoved; alternateCombined sources1 Publication
ChainiPRO_00002140292 – 528Golgi resident protein GCP60, N-terminally processedAdd BLAST527

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanine; in Golgi resident protein GCP60, N-terminally processedCombined sources1
Modified residuei13PhosphoserineCombined sources1
Modified residuei18PhosphothreonineCombined sources1
Modified residuei20PhosphoserineCombined sources1
Modified residuei43PhosphoserineCombined sources1
Modified residuei47PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9H3P7.
MaxQBiQ9H3P7.
PaxDbiQ9H3P7.
PeptideAtlasiQ9H3P7.
PRIDEiQ9H3P7.

PTM databases

iPTMnetiQ9H3P7.
PhosphoSitePlusiQ9H3P7.
SwissPalmiQ9H3P7.

Expressioni

Tissue specificityi

Ubiquitous, with highest expression in testis and ovary.1 Publication

Gene expression databases

BgeeiENSG00000182827.
CleanExiHS_ACBD3.
ExpressionAtlasiQ9H3P7. baseline and differential.
GenevisibleiQ9H3P7. HS.

Organism-specific databases

HPAiHPA015594.

Interactioni

Subunit structurei

Interacts with the C-terminal cytoplasmic domain of giantin/GOLGB1 (PubMed:11590181). Interacts with PBR and PKA regulatory subunit RI-alpha. Does not interact with PKA regulatory subunit RI-beta nor PKA regulatory subunit RII-alpha (By similarity). Interacts with PI4KB, TBC1D22A AND TBC1D22B; interactions with PI4KB and with TBC1D22A and TBC1D22B are mutually exclusive (PubMed:23572552, PubMed:27009356, PubMed:27989622). Interacts with C10ORF76 and RAB11B (PubMed:23572552).By similarity4 Publications
(Microbial infection) Interacts with 3A proteins from various picornaviruses, including hepatitis A virus, human parechovirus 1, and human klassevirus. Kobuviral (Aichi) 3A protein binding may compete the physiological interaction with TBC1D22A, while enteroviral (poliovirus) 3A protein does not seem to interfere (PubMed:23572552). Interacts (via GOLD domain) with Kobuviral (Aichi) 3A protein (PubMed:27989622, PubMed:23572552).2 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • protein kinase A regulatory subunit binding Source: UniProtKB

Protein-protein interaction databases

BioGridi122262. 34 interactors.
DIPiDIP-40673N.
IntActiQ9H3P7. 18 interactors.
MINTiMINT-5003562.
STRINGi9606.ENSP00000355777.

Chemistry databases

BindingDBiQ9H3P7.

Structurei

Secondary structure

1528
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi243 – 265Combined sources23
Helixi270 – 304Combined sources35
Beta strandi373 – 378Combined sources6
Helixi380 – 387Combined sources8
Helixi391 – 393Combined sources3
Beta strandi394 – 397Combined sources4
Beta strandi402 – 408Combined sources7
Beta strandi414 – 421Combined sources8
Beta strandi423 – 425Combined sources3
Beta strandi427 – 434Combined sources8
Beta strandi444 – 448Combined sources5
Beta strandi476 – 485Combined sources10
Turni487 – 489Combined sources3
Beta strandi490 – 497Combined sources8
Beta strandi503 – 509Combined sources7
Beta strandi518 – 527Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2N72NMR-A241-308[»]
2N73NMR-A241-308[»]
5LZ1X-ray2.00A364-528[»]
5LZ3X-ray3.00A364-528[»]
5LZ6X-ray2.60A364-528[»]
5TDQX-ray2.49A367-528[»]
ProteinModelPortaliQ9H3P7.
SMRiQ9H3P7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini83 – 174ACBPROSITE-ProRule annotationAdd BLAST92
Domaini384 – 526GOLDPROSITE-ProRule annotationAdd BLAST143

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni241 – 308Interaction with PI4KB1 PublicationAdd BLAST68

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili174 – 257Sequence analysisAdd BLAST84

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi21 – 60Pro-richAdd BLAST40
Compositional biasi182 – 240Glu-richAdd BLAST59
Compositional biasi196 – 238Arg-richAdd BLAST43
Compositional biasi241 – 308Gln-richAdd BLAST68

Domaini

The GOLD domain is essential for giantin binding.

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG3878. Eukaryota.
ENOG410XS5V. LUCA.
GeneTreeiENSGT00530000063651.
HOVERGENiHBG051712.
InParanoidiQ9H3P7.
OMAiGSHSYPG.
OrthoDBiEOG091G0KJD.
PhylomeDBiQ9H3P7.
TreeFamiTF321667.

Family and domain databases

Gene3Di1.20.80.10. 1 hit.
InterProiView protein in InterPro
IPR022408. Acyl-CoA-binding_prot_CS.
IPR000582. Acyl-CoA-binding_protein.
IPR035984. Acyl-CoA-binding_sf.
IPR014352. FERM/acyl-CoA-bd_prot_sf.
IPR009038. GOLD_dom.
IPR036598. GOLD_dom_sf.
PfamiView protein in Pfam
PF00887. ACBP. 1 hit.
PF13897. GOLD_2. 1 hit.
SUPFAMiSSF101576. SSF101576. 2 hits.
SSF47027. SSF47027. 1 hit.
PROSITEiView protein in PROSITE
PS00880. ACB_1. 1 hit.
PS51228. ACB_2. 1 hit.
PS50866. GOLD. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9H3P7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAVLNAERL EVSVDGLTLS PDPEERPGAE GAPLLPPPLP PPSPPGSGRG
60 70 80 90 100
PGASGEQPEP GEAAAGGAAE EARRLEQRWG FGLEELYGLA LRFFKEKDGK
110 120 130 140 150
AFHPTYEEKL KLVALHKQVL MGPYNPDTCP EVGFFDVLGN DRRREWAALG
160 170 180 190 200
NMSKEDAMVE FVKLLNRCCH LFSTYVASHK IEKEEQEKKR KEEEERRRRE
210 220 230 240 250
EEERERLQKE EEKRRREEEE RLRREEEERR RIEEERLRLE QQKQQIMAAL
260 270 280 290 300
NSQTAVQFQQ YAAQQYPGNY EQQQILIRQL QEQHYQQYMQ QLYQVQLAQQ
310 320 330 340 350
QAALQKQQEV VVAGSSLPTS SKVNATVPSN MMSVNGQAKT HTDSSEKELE
360 370 380 390 400
PEAAEEALEN GPKESLPVIA APSMWTRPQI KDFKEKIQQD ADSVITVGRG
410 420 430 440 450
EVVTVRVPTH EEGSYLFWEF ATDNYDIGFG VYFEWTDSPN TAVSVHVSES
460 470 480 490 500
SDDDEEEEEN IGCEEKAKKN ANKPLLDEIV PVYRRDCHEE VYAGSHQYPG
510 520
RGVYLLKFDN SYSLWRSKSV YYRVYYTR
Length:528
Mass (Da):60,593
Last modified:January 23, 2007 - v4
Checksum:iB36EC550F8268FC2
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti93F → L in AAN60219 (Ref. 2) Curated1
Sequence conflicti230R → I in AAN60219 (Ref. 2) Curated1
Sequence conflicti265Q → R in AAN60219 (Ref. 2) Curated1
Sequence conflicti333S → P in AAN60219 (Ref. 2) Curated1
Sequence conflicti384 – 397KEKIQ…SVITV → QREDSAGCRFRDYS (Ref. 2) CuratedAdd BLAST14
Sequence conflicti425Y → C in AAN60219 (Ref. 2) Curated1
Sequence conflicti473K → R in AAH60792 (PubMed:15489334).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_019615187E → D2 PublicationsCorresponds to variant dbSNP:rs2306120Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB043587 mRNA. Translation: BAB20592.2.
AY150218 mRNA. Translation: AAN60219.1.
AK025520 mRNA. Translation: BAB15159.1.
AK314468 mRNA. Translation: BAG37076.1.
AL592045 Genomic DNA. No translation available.
CH471098 Genomic DNA. Translation: EAW69775.1.
BC034563 mRNA. Translation: AAH34563.2.
BC045533 mRNA. Translation: AAH45533.1.
BC060792 mRNA. Translation: AAH60792.1.
CCDSiCCDS1551.1.
RefSeqiNP_073572.2. NM_022735.3.
UniGeneiHs.520207.

Genome annotation databases

EnsembliENST00000366812; ENSP00000355777; ENSG00000182827.
GeneIDi64746.
KEGGihsa:64746.
UCSCiuc001hpy.4. human.

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiGCP60_HUMAN
AccessioniPrimary (citable) accession number: Q9H3P7
Secondary accession number(s): B2RB29
, Q5VTJ0, Q6P9F1, Q8IZC5, Q8N4D6, Q9H6U3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: January 23, 2007
Last modified: November 22, 2017
This is version 144 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references