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Protein

tRNA dimethylallyltransferase

Gene

TRIT1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 of both cytosolic and mitochondrial tRNAs, leading to the formation of N6-(dimethylallyl)adenosine (i6A).1 Publication

Catalytic activityi

Dimethylallyl diphosphate + adenine(37) in tRNA = diphosphate + N6-dimethylallyladenine(37) in tRNA.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri395 – 425Matrin-typeAdd BLAST31

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processtRNA processing
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BRENDAi2.5.1.75 2681
ReactomeiR-HSA-6782315 tRNA modification in the nucleus and cytosol
R-HSA-6787450 tRNA modification in the mitochondrion

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA dimethylallyltransferase (EC:2.5.1.75)
Alternative name(s):
Isopentenyl-diphosphate:tRNA isopentenyltransferase
Short name:
IPP transferase
Short name:
IPPT
hGRO1
tRNA isopentenyltransferase 1
Short name:
IPTase
Gene namesi
Name:TRIT1
Synonyms:IPT, MOD5
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

EuPathDBiHostDB:ENSG00000043514.15
HGNCiHGNC:20286 TRIT1
MIMi617840 gene
neXtProtiNX_Q9H3H1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Mitochondrion

Pathology & Biotechi

Organism-specific databases

DisGeNETi54802
OpenTargetsiENSG00000043514
PharmGKBiPA134943037

Polymorphism and mutation databases

BioMutaiTRIT1
DMDMi56405066

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 47MitochondrionSequence analysisAdd BLAST47
ChainiPRO_000001902348 – 467tRNA dimethylallyltransferaseAdd BLAST420

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei443PhosphoserineCombined sources1
Modified residuei455PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9H3H1
MaxQBiQ9H3H1
PaxDbiQ9H3H1
PeptideAtlasiQ9H3H1
PRIDEiQ9H3H1

PTM databases

iPTMnetiQ9H3H1
PhosphoSitePlusiQ9H3H1

Expressioni

Gene expression databases

BgeeiENSG00000043514
CleanExiHS_TRIT1
ExpressionAtlasiQ9H3H1 baseline and differential
GenevisibleiQ9H3H1 HS

Organism-specific databases

HPAiHPA024174

Interactioni

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei122Interaction with substrate tRNABy similarity1
Sitei206Interaction with substrate tRNABy similarity1

Protein-protein interaction databases

BioGridi120161, 4 interactors
IntActiQ9H3H1, 4 interactors
MINTiQ9H3H1
STRINGi9606.ENSP00000321810

Structurei

3D structure databases

ProteinModelPortaliQ9H3H1
SMRiQ9H3H1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni32 – 37Substrate bindingBy similarity6
Regioni55 – 58Interaction with substrate tRNABy similarity4
Regioni183 – 187Interaction with substrate tRNABy similarity5
Regioni233 – 255Interaction with isopentenylpyrophosphate transferaseBy similarityAdd BLAST23
Regioni281 – 283Interaction with substrate tRNABy similarity3
Regioni313 – 331Interaction with substrate tRNABy similarityAdd BLAST19
Regioni323 – 330Interaction with substrate tRNABy similarity8

Sequence similaritiesi

Belongs to the IPP transferase family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri395 – 425Matrin-typeAdd BLAST31

Keywords - Domaini

Transit peptide, Zinc-finger

Phylogenomic databases

eggNOGiKOG1384 Eukaryota
COG0324 LUCA
GeneTreeiENSGT00390000015214
HOVERGENiHBG052492
InParanoidiQ9H3H1
KOiK00791
OMAiGTHYYTQ
OrthoDBiEOG091G0VY8
PhylomeDBiQ9H3H1
TreeFamiTF315069

Family and domain databases

HAMAPiMF_00185 IPP_trans, 1 hit
InterProiView protein in InterPro
IPR030666 IPP_transferase_euk
IPR018022 IPT
IPR003604 Matrin/U1-like-C_Znf_C2H2
IPR027417 P-loop_NTPase
IPR022755 Znf_C2H2_jaz
IPR036236 Znf_C2H2_sf
PfamiView protein in Pfam
PF01715 IPPT, 1 hit
PF12171 zf-C2H2_jaz, 1 hit
PIRSFiPIRSF039110 IPP_transferase, 1 hit
SMARTiView protein in SMART
SM00451 ZnF_U1, 1 hit
SUPFAMiSSF52540 SSF52540, 2 hits
SSF57667 SSF57667, 1 hit
TIGRFAMsiTIGR00174 miaA, 1 hit

Sequences (6)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 6 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9H3H1-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MASVAAARAV PVGSGLRGLQ RTLPLVVILG ATGTGKSTLA LQLGQRLGGE
60 70 80 90 100
IVSADSMQVY EGLDIITNKV SAQEQRICRH HMISFVDPLV TNYTVVDFRN
110 120 130 140 150
RATALIEDIF ARDKIPIVVG GTNYYIESLL WKVLVNTKPQ EMGTEKVIDR
160 170 180 190 200
KVELEKEDGL VLHKRLSQVD PEMAAKLHPH DKRKVARSLQ VFEETGISHS
210 220 230 240 250
EFLHRQHTEE GGGPLGGPLK FSNPCILWLH ADQAVLDERL DKRVDDMLAA
260 270 280 290 300
GLLEELRDFH RRYNQKNVSE NSQDYQHGIF QSIGFKEFHE YLITEGKCTL
310 320 330 340 350
ETSNQLLKKG IEALKQVTKR YARKQNRWVK NRFLSRPGPI VPPVYGLEVS
360 370 380 390 400
DVSKWEESVL EPALEIVQSF IQGHKPTATP IKMPYNEAEN KRSYHLCDLC
410 420 430 440 450
DRIIIGDREW AAHIKSKSHL NQLKKRRRLD SDAVNTIESQ SVSPDHNKEP
460
KEKGSPGQND QELKCSV
Length:467
Mass (Da):52,725
Last modified:March 1, 2001 - v1
Checksum:i634469919D7F56A5
GO
Isoform 2 (identifier: Q9H3H1-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-141: Missing.

Note: No experimental confirmation available.
Show »
Length:326
Mass (Da):37,435
Checksum:iEAB3F0F9664B7ACE
GO
Isoform 3 (identifier: Q9H3H1-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-141: Missing.
     235-236: Missing.

Note: No experimental confirmation available.
Show »
Length:324
Mass (Da):37,223
Checksum:i1E6835D7C09126A9
GO
Isoform 4 (identifier: Q9H3H1-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     311-336: Missing.

Show »
Length:441
Mass (Da):49,453
Checksum:i28DB69A67BD6C4B8
GO
Isoform 5 (identifier: Q9H3H1-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     59-138: Missing.
     235-236: Missing.

Note: No experimental confirmation available.
Show »
Length:385
Mass (Da):43,305
Checksum:iD1282514B2CDCF8A
GO
Isoform 6 (identifier: Q9H3H1-6) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-5: MASVA → MFRKI
     6-309: Missing.

Show »
Length:163
Mass (Da):18,758
Checksum:iF66F2BFD015B21F5
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti303S → G in CAG33507 (Ref. 5) Curated1
Sequence conflicti446H → Y in AAL14107 (PubMed:11560893).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_020486202F → L1 PublicationCorresponds to variant dbSNP:rs3738671Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0107191 – 141Missing in isoform 2 and isoform 3. 2 PublicationsAdd BLAST141
Alternative sequenceiVSP_0537461 – 5MASVA → MFRKI in isoform 6. 1 Publication5
Alternative sequenceiVSP_0537476 – 309Missing in isoform 6. 1 PublicationAdd BLAST304
Alternative sequenceiVSP_01241559 – 138Missing in isoform 5. 1 PublicationAdd BLAST80
Alternative sequenceiVSP_010720235 – 236Missing in isoform 3 and isoform 5. 2 Publications2
Alternative sequenceiVSP_010721311 – 336Missing in isoform 4. 1 PublicationAdd BLAST26

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF074918 Transcribed RNA Translation: AAG31324.1
AY702944 mRNA Translation: AAW63405.1
AY702945 mRNA Translation: AAW63406.1
AY303390 mRNA Translation: AAP60111.1
AK000068 mRNA Translation: BAA90923.1
CR457226 mRNA Translation: CAG33507.1
AL033527 Genomic DNA No translation available.
BC010741 mRNA Translation: AAH10741.2
BC107569 mRNA Translation: AAI07570.1
BC128155 mRNA Translation: AAI28156.1
AY052768 mRNA Translation: AAL14107.1
CCDSiCCDS30681.1 [Q9H3H1-1]
CCDS81302.1 [Q9H3H1-5]
CCDS81303.1 [Q9H3H1-4]
RefSeqiNP_001299620.1, NM_001312691.1 [Q9H3H1-4]
NP_001299621.1, NM_001312692.1 [Q9H3H1-5]
NP_060116.2, NM_017646.5 [Q9H3H1-1]
XP_006710769.1, XM_006710706.1 [Q9H3H1-2]
UniGeneiHs.356554

Genome annotation databases

EnsembliENST00000316891; ENSP00000321810; ENSG00000043514 [Q9H3H1-1]
ENST00000372818; ENSP00000361905; ENSG00000043514 [Q9H3H1-4]
ENST00000441669; ENSP00000388333; ENSG00000043514 [Q9H3H1-5]
ENST00000537440; ENSP00000437700; ENSG00000043514 [Q9H3H1-6]
GeneIDi54802
KEGGihsa:54802
UCSCiuc001cem.5 human [Q9H3H1-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiMOD5_HUMAN
AccessioniPrimary (citable) accession number: Q9H3H1
Secondary accession number(s): A1A4X7
, Q3T7B5, Q5QPK5, Q5QPK6, Q6IAC9, Q96FJ3, Q96L45, Q9NXT7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 5, 2004
Last sequence update: March 1, 2001
Last modified: March 28, 2018
This is version 142 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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