Q9H361 (PABP3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Polyadenylate-binding protein 3
Short name=PABP-3
Short name=Poly(A)-binding protein 3

Alternative name(s):
Testis-specific poly(A)-binding protein

Gene names

Name:	PABPC3
Synonyms:	PABP3, PABPL3

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	631 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Binds the poly(A) tail of mRNA. May be involved in cytoplasmic regulatory processes of mRNA metabolism. Binds poly(A) with a slightly lower affinity as compared to PABPC1.
Subcellular location	Cytoplasm By similarity.
Tissue specificity	Testis specific. Ref.1
Sequence similarities	Belongs to the polyadenylate-binding protein type-1 family. Contains 1 PABC domain. Contains 4 RRM (RNA recognition motif) domains.

Ontologies

Keywords
Cellular component	Cytoplasm
Domain	Repeat
Ligand	RNA-binding
PTM	Acetylation Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	mRNA metabolic process Non-traceable author statement Ref.1. Source: UniProtKB
Cellular component	cytoplasm Non-traceable author statement Ref.1. Source: UniProtKB
Molecular function	nucleotide binding Inferred from electronic annotation. Source: InterPro poly(A) RNA binding Inferred from direct assay Ref.1. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 631

631

Polyadenylate-binding protein 3

PRO_0000081702

Regions

Domain

11 – 89

79

RRM 1

Domain

99 – 175

77

RRM 2

Domain

191 – 268

78

RRM 3

Domain

294 – 370

77

RRM 4

Domain

537 – 614

78

PABC

Amino acid modifications

Modified residue

213

1

N6-acetyllysine Ref.5

Modified residue

259

1

N6-acetyllysine Ref.5

Modified residue

364

1

Phosphotyrosine Ref.4

Experimental info

Sequence conflict

221 – 222

2

KV → EL in CAB66834. Ref.2

Sequence conflict

492

1

A → R in AAG38953. Ref.1

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

631

Helix Strand Turn

Details...

Beta strand

296 – 299

4

Helix

307 – 312

6

Turn

313 – 316

4

Beta strand

320 – 323

4

Beta strand

338 – 342

5

Helix

343 – 353

11

Beta strand

357 – 362

6

Beta strand

364 – 367

4

Sequences

	Sequence		Length	Mass (Da)
	Q9H361 [UniParc]. Last modified February 1, 2003. Version 2. Checksum: 2F8C5BF6F508AAEF	FASTA	631	70,031
	10 20 30 40 50 60 MNPSTPSYPT ASLYVGDLHP DVTEAMLYEK FSPAGPILSI RICRDLITSG SSNYAYVNFQ 70 80 90 100 110 120 HTKDAEHALD TMNFDVIKGK PVRIMWSQRD PSLRKSGVGN IFVKNLDKSI NNKALYDTVS 130 140 150 160 170 180 AFGNILSCNV VCDENGSKGY GFVHFETHEA AERAIKKMNG MLLNGRKVFV GQFKSRKERE 190 200 210 220 230 240 AELGARAKEF PNVYIKNFGE DMDDERLKDL FGKFGPALSV KVMTDESGKS KGFGFVSFER 250 260 270 280 290 300 HEDAQKAVDE MNGKELNGKQ IYVGRAQKKV ERQTELKRTF EQMKQDRITR YQVVNLYVKN 310 320 330 340 350 360 LDDGIDDERL RKAFSPFGTI TSAKVMMEGG RSKGFGFVCF SSPEEATKAV TEMNGRIVAT 370 380 390 400 410 420 KPLYVALAQR KEERQAYLTN EYMQRMASVR AVPNQRAPPS GYFMTAVPQT QNHAAYYPPS 430 440 450 460 470 480 QIARLRPSPR WTAQGARPHP FQNKPSAIRP GAPRVPFSTM RPASSQVPRV MSTQRVANTS 490 500 510 520 530 540 TQTVGPRPAA AAAAAATPAV RTVPRYKYAA GVRNPQQHRN AQPQVTMQQL AVHVQGQETL 550 560 570 580 590 600 TASRLASAPP QKQKQMLGER LFPLIQAMHP TLAGKITGML LEIDNSELLY MLESPESLRS 610 620 630 KVDEAVAVLQ AHQAKEATQK AVNSATGVPT V

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Human testis expresses a specific poly(A)-binding protein." Feral C., Guellaen G., Pawlak A. Nucleic Acids Res. 29:1872-1883(2001) [PubMed: 11328870] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION, TISSUE SPECIFICITY. Tissue: Testis.
[2]	"Towards a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs." Wiemann S., Weil B., Wellenreuther R., Gassenhuber J., Glassl S., Ansorge W., Boecher M., Bloecker H., Bauersachs S., Blum H., Lauber J., Duesterhoeft A., Beyer A., Koehrer K., Strack N., Mewes H.-W., Ottenwaelder B., Obermaier B. , Tampe J., Heubner D., Wambutt R., Korn B., Klein M., Poustka A. Genome Res. 11:422-435(2001) [PubMed: 11230166] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Testis.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Testis.
[4]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed: 19690332] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-364, MASS SPECTROMETRY. Tissue: Leukemic T-cell.
[5]	"Lysine acetylation targets protein complexes and co-regulates major cellular functions." Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T., Olsen J.V., Mann M. Science 325:834-840(2009) [PubMed: 19608861] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-213 AND LYS-259, MASS SPECTROMETRY.
[6]	"Solution structure of RNA binding domain 4 in polyadenylation binding protein 3." RIKEN structural genomics initiative (RSGI) Submitted (JUN-2006) to the PDB data bank Cited for: STRUCTURE BY NMR OF 286-376.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF132026 mRNA. Translation: AAG38953.1.
AL136900 mRNA. Translation: CAB66834.2.
BC027617 mRNA. Translation: AAH27617.1.

IPI

IPI00301154.

RefSeq

NP_112241.2. NM_030979.2.

UniGene

Hs.458280.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2D9P	NMR	-	A	286-375	[»]

ProteinModelPortal

Q9H361.

SMR

Q9H361. Positions 11-376, 491-631.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q9H361. 2 interactions.

STRING

Q9H361.

PTM databases

PhosphoSite

Q9H361.

Polymorphism databases

DMDM

28201852.

Proteomic databases

PRIDE

Q9H361.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000281589; ENSP00000281589; ENSG00000151846.

GeneID

5042.

KEGG

hsa:5042.

UCSC

uc001upy.1. human.

Organism-specific databases

CTD

5042.

GeneCards

GC13P025670.

H-InvDB

HIX0011181.

HGNC

HGNC:8556. PABPC3.

MIM

604680. gene.

neXtProt

NX_Q9H361.

GenAtlas

Search...

Phylogenomic databases

GeneTree

ENSGT00590000082738.

HOGENOM

HBG756718.

HOVERGEN

HBG002295.

InParanoid

Q9H361.

OMA

NEYMQRM.

OrthoDB

EOG4KH2TN.

PhylomeDB

Q9H361.

Gene expression databases

ArrayExpress

Q9H361.

Bgee

Q9H361.

CleanEx

HS_PABPC3.

Genevestigator

Q9H361.

GermOnline

ENSG00000151846. Homo sapiens.

Family and domain databases

InterPro

IPR012677. Nucleotide-bd_a/b_plait.
IPR006515. PABP_1234.
IPR002004. PABP_HYD.
IPR000504. RRM_dom.
[Graphical view]

Gene3D

G3DSA:3.30.70.330. a_b_plait_nuc_bd. 4 hits.
G3DSA:1.10.1900.10. PABP_HYD. 1 hit.

KO

K13126.

Pfam

PF00658. PABP. 1 hit.
PF00076. RRM_1. 4 hits.
[Graphical view]

SMART

SM00517. PolyA. 1 hit.
SM00360. RRM. 4 hits.
[Graphical view]

SUPFAM

SSF63570. PABP_HYD. 1 hit.

TIGRFAMs

TIGR01628. PABP-1234. 1 hit.

PROSITE

PS51309. PABC. 1 hit.
PS50102. RRM. 4 hits.
[Graphical view]

ProtoNet

Search...

Other

NextBio

19414.

SOURCE

Search...

Entry information

Entry name

PABP3_HUMAN

Accession

Primary (citable) accession number: Q9H361
Secondary accession number(s): Q8NHV0, Q9H086

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 2003
Last sequence update:	February 1, 2003
Last modified:	January 25, 2012
This is version 92 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.