Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Band 4.1-like protein 4B

Gene

EPB41L4B

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Up-regulates the activity of the Rho guanine nucleotide exchange factor ARHGEF18 (By similarity). Involved in the regulation of the circumferential actomyosin belt in epithelial cells (PubMed:22006950). Promotes cellular adhesion, migration and motility in vitro and may play a role in wound healing (PubMed:23664528). May have a role in mediating cytoskeletal changes associated with steroid-induced cell differentiation (PubMed:14521927).By similarity3 Publications

GO - Molecular functioni

  • structural constituent of cytoskeleton Source: ProtInc

GO - Biological processi

  • actomyosin structure organization Source: MGI
  • positive regulation of cell adhesion Source: UniProtKB
  • positive regulation of gene expression Source: UniProtKB
  • positive regulation of keratinocyte migration Source: UniProtKB
  • wound healing Source: UniProtKB
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Band 4.1-like protein 4B
Alternative name(s):
FERM-containing protein CG1
Protein EHM2
Gene namesi
Name:EPB41L4B
Synonyms:EHM2, LULU21 Publication
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 9

Organism-specific databases

HGNCiHGNC:19818. EPB41L4B.

Subcellular locationi

  • Cytoplasm 1 Publication
  • Cell junctiontight junction 1 Publication

  • Note: Accumulates along apical cell-cell boundaries and is also detected in the cytoplasm in a punctate manner.1 Publication

GO - Cellular componenti

  • apical part of cell Source: MGI
  • bicellular tight junction Source: UniProtKB-SubCell
  • cytoplasm Source: UniProtKB
  • cytoskeleton Source: ProtInc
  • extrinsic component of membrane Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cytoplasm, Tight junction

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134986250.

Polymorphism and mutation databases

BioMutaiEPB41L4B.
DMDMi209572611.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 900900Band 4.1-like protein 4BPRO_0000219404Add
BLAST

Post-translational modificationi

May be negatively regulated by phosphorylation.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9H329.
MaxQBiQ9H329.
PaxDbiQ9H329.
PeptideAtlasiQ9H329.
PRIDEiQ9H329.

PTM databases

iPTMnetiQ9H329.
PhosphoSiteiQ9H329.

Expressioni

Tissue specificityi

Expressed at higher levels in acute wounds than chronic wounds with increased expression in healing wounds, especially at the leading wound edge (PubMed:23664528). Isoform 1 is highly expressed in brain. Isoform 2 is highly expressed in testis with lower levels in prostate and breast (PubMed:14521927).2 Publications

Inductioni

By the androgen dihydrotestosterone (DHT).1 Publication

Gene expression databases

BgeeiENSG00000095203.
CleanExiHS_EPB41L4B.
GenevisibleiQ9H329. HS.

Organism-specific databases

HPAiHPA042862.

Interactioni

Subunit structurei

Interacts (via FERM domain) with ARHGEF18 (via C-terminus); the interaction activates ARHGEF18.By similarity

Protein-protein interaction databases

BioGridi120044. 16 interactions.
IntActiQ9H329. 12 interactions.
STRINGi9606.ENSP00000363694.

Structurei

3D structure databases

ProteinModelPortaliQ9H329.
SMRiQ9H329. Positions 84-367.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini85 – 369285FERMPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi504 – 51714His-richAdd
BLAST

Sequence similaritiesi

Contains 1 FERM domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG410IQ5Y. Eukaryota.
ENOG410YG1S. LUCA.
GeneTreeiENSGT00760000118823.
HOGENOMiHOG000231632.
HOVERGENiHBG051434.
InParanoidiQ9H329.
OMAiDPNVRSP.
OrthoDBiEOG091G032O.
PhylomeDBiQ9H329.
TreeFamiTF319780.

Family and domain databases

Gene3Di1.20.80.10. 1 hit.
2.30.29.30. 1 hit.
InterProiIPR030698. Band4.1-like4B.
IPR019749. Band_41_domain.
IPR000798. Ez/rad/moesin-like.
IPR014847. FERM-adjacent.
IPR014352. FERM/acyl-CoA-bd_prot_3-hlx.
IPR019748. FERM_central.
IPR019747. FERM_CS.
IPR000299. FERM_domain.
IPR018979. FERM_N.
IPR018980. FERM_PH-like_C.
IPR011993. PH_dom-like.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PANTHERiPTHR23280:SF18. PTHR23280:SF18. 2 hits.
PfamiPF08736. FA. 1 hit.
PF09380. FERM_C. 1 hit.
PF00373. FERM_M. 1 hit.
PF09379. FERM_N. 1 hit.
[Graphical view]
PRINTSiPR00935. BAND41.
PR00661. ERMFAMILY.
SMARTiSM00295. B41. 1 hit.
SM01195. FA. 1 hit.
SM01196. FERM_C. 1 hit.
[Graphical view]
SUPFAMiSSF47031. SSF47031. 1 hit.
SSF50729. SSF50729. 1 hit.
SSF54236. SSF54236. 1 hit.
PROSITEiPS00661. FERM_2. 1 hit.
PS50057. FERM_3. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9H329-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLRFLRRTFG RRSMQRYARG AAGRGAAGLG DERDGGPRGG PAAAASSSAL
60 70 80 90 100
PAAPGGSVFP AGGGPLLTGG AAVHISAAGA AKATLYCRVF LLDGTEVSVD
110 120 130 140 150
LPKHAKGQDL FDQIVYHLDL VETDYFGLQF LDSAQVAHWL DHAKPIKKQM
160 170 180 190 200
KIGPAYALHF RVKYYSSEPN NLREEFTRYL FVLQLRHDIL SGKLKCPYET
210 220 230 240 250
AVELAALCLQ AELGECELPE HTPELVSEFR FIPNQTEAME FDIFQRWKEC
260 270 280 290 300
RGKSPAQAEL SYLNKAKWLE MYGVDMHVVR GRDGCEYSLG LTPTGILIFE
310 320 330 340 350
GANKIGLFFW PKITKMDFKK SKLTLVVVED DDQGREQEHT FVFRLDSART
360 370 380 390 400
CKHLWKCAVE HHAFFRLRTP GNSKSNRSDF IRLGSRFRFS GRTEYQATHG
410 420 430 440 450
SRLRRTSTFE RKPSKRYPSR RHSTFKASNP VIAAQLCSKT NPEVHNYQPQ
460 470 480 490 500
YHPNIHPSQP RWHPHSPNVS YPLPSPVLSS SDRLPFGIEE NGGTPFLTAA
510 520 530 540 550
SGRHHHQHQH QHQHQHHSNY SLSLTLENKE GPLRSPNSSS KSLTKLSPGT
560 570 580 590 600
PALFSEAAAH LKKLELETVK AAGPWPPLHI NINKAEEKKV SEKTLQTPLL
610 620 630 640 650
PSPVADHVKC NILKAQLENA SRVNIQGGKE ESPFVNINKK SSLQDASVRS
660 670 680 690 700
PIPIRVETAQ PAVEKPEIKP PRVRKLTRQY SFDEDDLPPD LAEAVGVTTS
710 720 730 740 750
TTTNTTTAAT QVSVPLPSPK VQNVSSPHKS EGKGLLSPGA KSPSDRGGAF
760 770 780 790 800
TLEPGDLLMD FTEATPLAEP ASNPHCAHSR CSPPLSLPMK EETTGVCMYP
810 820 830 840 850
PIKTRLIKTF PVDTMNPFPD TFTTGPQFTA DFRDSKLQCC PGPTSPLIPA
860 870 880 890 900
ATLRPLTETV STVQTIYTTR KPVSLAASAE TLRQELEREK MMKRLLMTEL
Length:900
Mass (Da):99,712
Last modified:October 14, 2008 - v2
Checksum:i9A4BD658DBE65BDE
GO
Isoform 2 (identifier: Q9H329-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     470-518: SYPLPSPVLS...QHQHQHQHHS → RPSFQDDRSH...MYRDKLMTAL
     519-900: Missing.

Show »
Length:518
Mass (Da):58,566
Checksum:i7CF6295CD2BE4E21
GO

Sequence cautioni

The sequence AAG43366 differs from that shown. Reason: Frameshift at positions 19, 35 and 870. Curated
The sequence AAG43368 differs from that shown. Reason: Frameshift at positions 19 and 35. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti73 – 731V → M in BAA96079 (PubMed:10783258).Curated
Sequence conflicti899 – 8991E → R in AAG43366 (PubMed:10603000).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti816 – 8161N → T.1 Publication
Corresponds to variant rs3750450 [ dbSNP | Ensembl ].
VAR_048356

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei470 – 51849SYPLP…HQHHS → RPSFQDDRSHWKASASGDDS HFDYVHDQNQKNLGGMQSMM YRDKLMTAL in isoform 2. 2 PublicationsVSP_007202Add
BLAST
Alternative sequencei519 – 900382Missing in isoform 2. 2 PublicationsVSP_007203Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF153416 mRNA. Translation: AAG43366.1. Frameshift.
AF153418 mRNA. Translation: AAG43368.1. Frameshift.
AB032179 mRNA. Translation: BAA96079.2.
AL359963, AL358815 Genomic DNA. Translation: CAH71092.1.
AL359963, AL358815 Genomic DNA. Translation: CAH71093.1.
AL358815, AL359963 Genomic DNA. Translation: CAI15130.1.
AL358815, AL359963 Genomic DNA. Translation: CAI15129.1.
CCDSiCCDS43859.1. [Q9H329-1]
CCDS43860.1. [Q9H329-2]
RefSeqiNP_060894.2. NM_018424.3. [Q9H329-2]
NP_061987.3. NM_019114.4. [Q9H329-1]
UniGeneiHs.591901.

Genome annotation databases

EnsembliENST00000374557; ENSP00000363685; ENSG00000095203. [Q9H329-2]
ENST00000374566; ENSP00000363694; ENSG00000095203. [Q9H329-1]
GeneIDi54566.
KEGGihsa:54566.
UCSCiuc004bdz.3. human. [Q9H329-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF153416 mRNA. Translation: AAG43366.1. Frameshift.
AF153418 mRNA. Translation: AAG43368.1. Frameshift.
AB032179 mRNA. Translation: BAA96079.2.
AL359963, AL358815 Genomic DNA. Translation: CAH71092.1.
AL359963, AL358815 Genomic DNA. Translation: CAH71093.1.
AL358815, AL359963 Genomic DNA. Translation: CAI15130.1.
AL358815, AL359963 Genomic DNA. Translation: CAI15129.1.
CCDSiCCDS43859.1. [Q9H329-1]
CCDS43860.1. [Q9H329-2]
RefSeqiNP_060894.2. NM_018424.3. [Q9H329-2]
NP_061987.3. NM_019114.4. [Q9H329-1]
UniGeneiHs.591901.

3D structure databases

ProteinModelPortaliQ9H329.
SMRiQ9H329. Positions 84-367.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi120044. 16 interactions.
IntActiQ9H329. 12 interactions.
STRINGi9606.ENSP00000363694.

PTM databases

iPTMnetiQ9H329.
PhosphoSiteiQ9H329.

Polymorphism and mutation databases

BioMutaiEPB41L4B.
DMDMi209572611.

Proteomic databases

EPDiQ9H329.
MaxQBiQ9H329.
PaxDbiQ9H329.
PeptideAtlasiQ9H329.
PRIDEiQ9H329.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000374557; ENSP00000363685; ENSG00000095203. [Q9H329-2]
ENST00000374566; ENSP00000363694; ENSG00000095203. [Q9H329-1]
GeneIDi54566.
KEGGihsa:54566.
UCSCiuc004bdz.3. human. [Q9H329-1]

Organism-specific databases

CTDi54566.
GeneCardsiEPB41L4B.
HGNCiHGNC:19818. EPB41L4B.
HPAiHPA042862.
MIMi610340. gene.
neXtProtiNX_Q9H329.
PharmGKBiPA134986250.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IQ5Y. Eukaryota.
ENOG410YG1S. LUCA.
GeneTreeiENSGT00760000118823.
HOGENOMiHOG000231632.
HOVERGENiHBG051434.
InParanoidiQ9H329.
OMAiDPNVRSP.
OrthoDBiEOG091G032O.
PhylomeDBiQ9H329.
TreeFamiTF319780.

Miscellaneous databases

ChiTaRSiEPB41L4B. human.
GenomeRNAii54566.
PROiQ9H329.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000095203.
CleanExiHS_EPB41L4B.
GenevisibleiQ9H329. HS.

Family and domain databases

Gene3Di1.20.80.10. 1 hit.
2.30.29.30. 1 hit.
InterProiIPR030698. Band4.1-like4B.
IPR019749. Band_41_domain.
IPR000798. Ez/rad/moesin-like.
IPR014847. FERM-adjacent.
IPR014352. FERM/acyl-CoA-bd_prot_3-hlx.
IPR019748. FERM_central.
IPR019747. FERM_CS.
IPR000299. FERM_domain.
IPR018979. FERM_N.
IPR018980. FERM_PH-like_C.
IPR011993. PH_dom-like.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PANTHERiPTHR23280:SF18. PTHR23280:SF18. 2 hits.
PfamiPF08736. FA. 1 hit.
PF09380. FERM_C. 1 hit.
PF00373. FERM_M. 1 hit.
PF09379. FERM_N. 1 hit.
[Graphical view]
PRINTSiPR00935. BAND41.
PR00661. ERMFAMILY.
SMARTiSM00295. B41. 1 hit.
SM01195. FA. 1 hit.
SM01196. FERM_C. 1 hit.
[Graphical view]
SUPFAMiSSF47031. SSF47031. 1 hit.
SSF50729. SSF50729. 1 hit.
SSF54236. SSF54236. 1 hit.
PROSITEiPS00661. FERM_2. 1 hit.
PS50057. FERM_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiE41LB_HUMAN
AccessioniPrimary (citable) accession number: Q9H329
Secondary accession number(s): Q5T4G5
, Q5T4G6, Q9H328, Q9P2V3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 23, 2003
Last sequence update: October 14, 2008
Last modified: September 7, 2016
This is version 127 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 9
    Human chromosome 9: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.