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Protein

Pinin

Gene

PNN

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcriptional activator binding to the E-box 1 core sequence of the E-cadherin promoter gene; the core-binding sequence is 5'CAGGTG-3'. Capable of reversing CTBP1-mediated transcription repression. Auxiliary component of the splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of core proteins and several peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. Participates in the regulation of alternative pre-mRNA splicing. Associates to spliced mRNA within 60 nt upstream of the 5'-splice sites. Component of the PSAP complex which binds RNA in a sequence-independent manner and is proposed to be recruited to the EJC prior to or during the splicing process and to regulate specific excision of introns in specific transcription subsets. Involved in the establishment and maintenance of epithelia cell-cell adhesion. Potential tumor suppressor for renal cell carcinoma.5 Publications

GO - Molecular functioni

  • DNA binding Source: UniProtKB-KW
  • poly(A) RNA binding Source: UniProtKB
  • structural molecule activity Source: ProtInc

GO - Biological processi

  • cell adhesion Source: ProtInc
  • mRNA splicing, via spliceosome Source: UniProtKB
  • regulation of transcription, DNA-templated Source: UniProtKB-KW
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

mRNA processing, mRNA splicing, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Protein family/group databases

TCDBi3.A.18.1.1. the nuclear mrna exporter (mrna-e) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Pinin
Alternative name(s):
140 kDa nuclear and cell adhesion-related phosphoprotein
Desmosome-associated protein
Domain-rich serine protein
Short name:
DRS protein
Short name:
DRSP
Melanoma metastasis clone A protein
Nuclear protein SDK3
SR-like protein
Gene namesi
Name:PNN
Synonyms:DRS, MEMA
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 14

Organism-specific databases

HGNCiHGNC:9162. PNN.

Subcellular locationi

GO - Cellular componenti

  • catalytic step 2 spliceosome Source: UniProtKB
  • cell-cell junction Source: ProtInc
  • desmosome Source: UniProtKB-SubCell
  • intermediate filament Source: ProtInc
  • membrane Source: UniProtKB
  • nuclear speck Source: UniProtKB-SubCell
  • nucleoplasm Source: HPA
  • plasma membrane Source: ProtInc
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Nucleus, Spliceosome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi8L → P: Abolishes interaction with KRT18. 1 Publication1
Mutagenesisi19L → P: Abolishes interaction with KRT18. 1 Publication1
Mutagenesisi502 – 503PE → AA: Abolishes interaction with CTBP1 and shows moderate relief of CTBP1-mediated repression. 1 Publication2

Keywords - Diseasei

Tumor suppressor

Organism-specific databases

DisGeNETi5411.
PharmGKBiPA33484.

Polymorphism and mutation databases

BioMutaiPNN.
DMDMi73921750.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources1 Publication
ChainiPRO_00001902422 – 717PininAdd BLAST716

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1 Publication1
Modified residuei48PhosphoserineCombined sources1
Modified residuei54Omega-N-methylarginineBy similarity1
Modified residuei58PhosphoserineCombined sources1
Modified residuei66PhosphoserineCombined sources1
Modified residuei96PhosphoserineCombined sources1
Modified residuei100PhosphoserineCombined sources1
Cross-linki109Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei114PhosphoserineCombined sources1
Modified residuei115PhosphoserineCombined sources1
Modified residuei124PhosphothreonineCombined sources1
Cross-linki157Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1)Combined sources
Cross-linki157Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei238N6-acetyllysine; alternateCombined sources1
Modified residuei238N6-succinyllysine; alternateBy similarity1
Cross-linki304Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei347PhosphoserineCombined sources1
Modified residuei375PhosphoserineCombined sources1
Modified residuei381PhosphoserineCombined sources1
Modified residuei441PhosphoserineCombined sources1
Modified residuei443PhosphoserineCombined sources1
Modified residuei450PhosphoserineCombined sources1
Cross-linki528Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei552PhosphoserineCombined sources1
Cross-linki553Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei658PhosphoserineCombined sources1
Modified residuei692PhosphoserineCombined sources1
Modified residuei695PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Isopeptide bond, Methylation, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ9H307.
MaxQBiQ9H307.
PaxDbiQ9H307.
PeptideAtlasiQ9H307.
PRIDEiQ9H307.

PTM databases

iPTMnetiQ9H307.
PhosphoSitePlusiQ9H307.
SwissPalmiQ9H307.

Expressioni

Tissue specificityi

Expressed in placenta, lung, liver, kidney, pancreas, spleen, thymus, prostate, testis, ovary, small intestine, colon, heart, epidermis, esophagus, brain and smooth and skeletal muscle. Expressed strongly in melanoma metastasis lesions and advanced primary tumors.3 Publications

Gene expression databases

BgeeiENSG00000100941.
CleanExiHS_PNN.
ExpressionAtlasiQ9H307. baseline and differential.
GenevisibleiQ9H307. HS.

Organism-specific databases

HPAiHPA001378.

Interactioni

Subunit structurei

Found in a mRNA splicing-dependent exon junction complex (EJC). Found in a complex with SR proteins. Found in a mRNP complex with RNPS1. Component of the PSAP complex consisting of RNPS1, SAP18 and PNN. Interacts with PNISR, CTBP1, CTBP2, KRT8, KRT18, KRT19, PS1D/PNO40, PPIG, RNPS1, SFRS4 and SRRM2. Identified in the spliceosome C complex.10 Publications

Protein-protein interaction databases

BioGridi111412. 105 interactors.
DIPiDIP-32950N.
IntActiQ9H307. 32 interactors.
MINTiMINT-1683388.
STRINGi9606.ENSP00000216832.

Structurei

3D structure databases

ProteinModelPortaliQ9H307.
SMRiQ9H307.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni2 – 284Necessary for interaction with RNPS1Add BLAST283
Regioni2 – 167Necessary for mediating alternative 5' splicingAdd BLAST166
Regioni2 – 98Necessary for interactions with KRT8, KRT18 and KRT19Add BLAST97
Regioni221 – 284Sufficient for PSAP complex assemblyAdd BLAST64
Regioni606 – 717Necessary for interaction with PPIG1 PublicationAdd BLAST112

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili2 – 32Sequence analysisAdd BLAST31
Coiled coili163 – 234Sequence analysisAdd BLAST72
Coiled coili287 – 379Sequence analysisAdd BLAST93
Coiled coili446 – 467Sequence analysisAdd BLAST22

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi172 – 471Glu-richAdd BLAST300
Compositional biasi469 – 520Gln-richAdd BLAST52
Compositional biasi552 – 704Ser-richAdd BLAST153
Compositional biasi632 – 717Arg-richAdd BLAST86

Sequence similaritiesi

Belongs to the pinin family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG3756. Eukaryota.
ENOG4110W63. LUCA.
HOVERGENiHBG053104.
InParanoidiQ9H307.
KOiK13114.
OrthoDBiEOG091G0S2R.
PhylomeDBiQ9H307.
TreeFamiTF331859.

Family and domain databases

InterProiIPR006786. Pinin_SDK_MemA.
IPR006787. Pinin_SDK_N.
[Graphical view]
PfamiPF04696. Pinin_SDK_memA. 1 hit.
PF04697. Pinin_SDK_N. 1 hit.
[Graphical view]
ProDomiPD011048. Pinin_SDK_N. 1 hit.
[Graphical view] [Entries sharing at least one domain]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9H307-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAVAVRTLQE QLEKAKESLK NVDENIRKLT GRDPNDVRPI QARLLALSGP
60 70 80 90 100
GGGRGRGSLL LRRGFSDSGG GPPAKQRDLE GAVSRLGGER RTRRESRQES
110 120 130 140 150
DPEDDDVKKP ALQSSVVATS KERTRRDLIQ DQNMDEKGKQ RNRRIFGLLM
160 170 180 190 200
GTLQKFKQES TVATERQKRR QEIEQKLEVQ AEEERKQVEN ERRELFEERR
210 220 230 240 250
AKQTELRLLE QKVELAQLQE EWNEHNAKII KYIRTKTKPH LFYIPGRMCP
260 270 280 290 300
ATQKLIEESQ RKMNALFEGR RIEFAEQINK MEARPRRQSM KEKEHQVVRN
310 320 330 340 350
EEQKAEQEEG KVAQREEELE ETGNQHNDVE IEEAGEEEEK EIAIVHSDAE
360 370 380 390 400
KEQEEEEQKQ EMEVKMEEET EVRESEKQQD SQPEEVMDVL EMVENVKHVI
410 420 430 440 450
ADQEVMETNR VESVEPSENE ASKELEPEME FEIEPDKECK SLSPGKENVS
460 470 480 490 500
ALDMEKESEE KEEKESEPQP EPVAQPQPQS QPQLQLQSQS QPVLQSQPPS
510 520 530 540 550
QPEDLSLAVL QPTPQVTQEQ GHLLPERKDF PVESVKLTEV PVEPVLTVHP
560 570 580 590 600
ESKSKTKTRS RSRGRARNKT SKSRSRSSSS SSSSSSSTSS SSGSSSSSGS
610 620 630 640 650
SSSRSSSSSS SSTSGSSSRD SSSSTSSSSE SRSRSRGRGH NRDRKHRRSV
660 670 680 690 700
DRKRRDTSGL ERSHKSSKGG SSRDTKGSKD KNSRSDRKRS ISESSRSGKR
710
SSRSERDRKS DRKDKRR
Length:717
Mass (Da):81,614
Last modified:January 23, 2007 - v4
Checksum:i1C05E1E12F54299F
GO
Isoform 2 (identifier: Q9H307-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-133: Missing.

Show »
Length:584
Mass (Da):67,005
Checksum:i7D96168629D5C4DA
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti69Missing in AAB48304 (PubMed:8922384).Curated1
Sequence conflicti168K → N in AAB48304 (PubMed:8922384).Curated1
Sequence conflicti268E → D in AAB48304 (PubMed:8922384).Curated1
Sequence conflicti303Q → H in AAB48304 (PubMed:8922384).Curated1
Sequence conflicti320E → V in AAB48304 (PubMed:8922384).Curated1
Sequence conflicti343A → G in AAB48304 (PubMed:8922384).Curated1
Sequence conflicti402 – 403DQ → EE in AAG33941 (PubMed:10645008).Curated2
Sequence conflicti459E → D in AAB48304 (PubMed:8922384).Curated1
Sequence conflicti478P → A in AAB48304 (PubMed:8922384).Curated1
Sequence conflicti491 – 492QP → EPQPQLQPEPAQPQLQSQPQ LQLQSQCHA AA sequence (PubMed:8922384).Curated2
Sequence conflicti497Q → H in AAB48304 (PubMed:8922384).Curated1
Sequence conflicti520Q → H in AAB48304 (PubMed:8922384).Curated1
Sequence conflicti523L → F in AAB48304 (PubMed:8922384).Curated1
Sequence conflicti541P → T AA sequence (PubMed:8922384).Curated1
Sequence conflicti543E → D in AAB48304 (PubMed:8922384).Curated1
Sequence conflicti547T → I AA sequence (PubMed:8922384).Curated1
Sequence conflicti550P → S AA sequence (PubMed:8922384).Curated1
Sequence conflicti551E → D AA sequence (PubMed:8922384).Curated1
Sequence conflicti553 – 557KSKTK → ESETN in AAB48304 (PubMed:8922384).Curated5
Sequence conflicti566A → T in AAB48304 (PubMed:8922384).Curated1
Sequence conflicti569 – 571KTS → RTT in AAB48304 (PubMed:8922384).Curated3
Sequence conflicti618S → G in AAF17209 (PubMed:10931946).Curated1
Sequence conflicti618S → G in CAA71377 (PubMed:9447706).Curated1
Sequence conflicti626S → STS in CAA70874 (PubMed:10095061).Curated1
Sequence conflicti664H → P in AAG33941 (PubMed:10645008).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_050540441S → T.Corresponds to variant rs2180792dbSNPEnsembl.1
Natural variantiVAR_023368671S → G.2 PublicationsCorresponds to variant rs13021dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0153071 – 133Missing in isoform 2. 2 PublicationsAdd BLAST133

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U77718 mRNA. Translation: AAB48304.1.
Y09703 mRNA. Translation: CAA70874.1.
AF195139 Genomic DNA. Translation: AAG33941.1.
AF112222 mRNA. Translation: AAF17209.1.
AK303136 mRNA. Translation: BAG64240.1.
AK223612 mRNA. Translation: BAD97332.1.
BC062602 mRNA. Translation: AAH62602.1.
Y10351 mRNA. Translation: CAA71377.1.
CCDSiCCDS9671.1. [Q9H307-1]
RefSeqiNP_002678.2. NM_002687.3. [Q9H307-1]
UniGeneiHs.409965.

Genome annotation databases

EnsembliENST00000216832; ENSP00000216832; ENSG00000100941.
GeneIDi5411.
KEGGihsa:5411.
UCSCiuc001wuw.5. human. [Q9H307-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U77718 mRNA. Translation: AAB48304.1.
Y09703 mRNA. Translation: CAA70874.1.
AF195139 Genomic DNA. Translation: AAG33941.1.
AF112222 mRNA. Translation: AAF17209.1.
AK303136 mRNA. Translation: BAG64240.1.
AK223612 mRNA. Translation: BAD97332.1.
BC062602 mRNA. Translation: AAH62602.1.
Y10351 mRNA. Translation: CAA71377.1.
CCDSiCCDS9671.1. [Q9H307-1]
RefSeqiNP_002678.2. NM_002687.3. [Q9H307-1]
UniGeneiHs.409965.

3D structure databases

ProteinModelPortaliQ9H307.
SMRiQ9H307.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi111412. 105 interactors.
DIPiDIP-32950N.
IntActiQ9H307. 32 interactors.
MINTiMINT-1683388.
STRINGi9606.ENSP00000216832.

Protein family/group databases

TCDBi3.A.18.1.1. the nuclear mrna exporter (mrna-e) family.

PTM databases

iPTMnetiQ9H307.
PhosphoSitePlusiQ9H307.
SwissPalmiQ9H307.

Polymorphism and mutation databases

BioMutaiPNN.
DMDMi73921750.

Proteomic databases

EPDiQ9H307.
MaxQBiQ9H307.
PaxDbiQ9H307.
PeptideAtlasiQ9H307.
PRIDEiQ9H307.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000216832; ENSP00000216832; ENSG00000100941.
GeneIDi5411.
KEGGihsa:5411.
UCSCiuc001wuw.5. human. [Q9H307-1]

Organism-specific databases

CTDi5411.
DisGeNETi5411.
GeneCardsiPNN.
H-InvDBHIX0037750.
HGNCiHGNC:9162. PNN.
HPAiHPA001378.
MIMi603154. gene.
neXtProtiNX_Q9H307.
PharmGKBiPA33484.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3756. Eukaryota.
ENOG4110W63. LUCA.
HOVERGENiHBG053104.
InParanoidiQ9H307.
KOiK13114.
OrthoDBiEOG091G0S2R.
PhylomeDBiQ9H307.
TreeFamiTF331859.

Miscellaneous databases

ChiTaRSiPNN. human.
GeneWikiiPinin.
GenomeRNAii5411.
PROiQ9H307.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000100941.
CleanExiHS_PNN.
ExpressionAtlasiQ9H307. baseline and differential.
GenevisibleiQ9H307. HS.

Family and domain databases

InterProiIPR006786. Pinin_SDK_MemA.
IPR006787. Pinin_SDK_N.
[Graphical view]
PfamiPF04696. Pinin_SDK_memA. 1 hit.
PF04697. Pinin_SDK_N. 1 hit.
[Graphical view]
ProDomiPD011048. Pinin_SDK_N. 1 hit.
[Graphical view] [Entries sharing at least one domain]
ProtoNetiSearch...

Entry informationi

Entry nameiPININ_HUMAN
AccessioniPrimary (citable) accession number: Q9H307
Secondary accession number(s): B4DZX8
, O60899, Q53EM7, Q6P5X4, Q7KYL1, Q99738, Q9UHZ9, Q9UQR9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 30, 2005
Last sequence update: January 23, 2007
Last modified: November 30, 2016
This is version 135 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 14
    Human chromosome 14: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.