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Protein

Pinin

Gene

PNN

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcriptional activator binding to the E-box 1 core sequence of the E-cadherin promoter gene; the core-binding sequence is 5'CAGGTG-3'. Capable of reversing CTBP1-mediated transcription repression. Auxiliary component of the splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of core proteins and several peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. Participates in the regulation of alternative pre-mRNA splicing. Associates to spliced mRNA within 60 nt upstream of the 5'-splice sites. Component of the PSAP complex which binds RNA in a sequence-independent manner and is proposed to be recruited to the EJC prior to or during the splicing process and to regulate specific excision of introns in specific transcription subsets. Involved in the establishment and maintenance of epithelia cell-cell adhesion. Potential tumor suppressor for renal cell carcinoma.5 Publications

GO - Molecular functioni

  • DNA binding Source: UniProtKB-KW
  • poly(A) RNA binding Source: UniProtKB
  • structural molecule activity Source: ProtInc

GO - Biological processi

  • cell adhesion Source: ProtInc
  • mRNA splicing, via spliceosome Source: UniProtKB
  • regulation of transcription, DNA-templated Source: UniProtKB-KW
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

mRNA processing, mRNA splicing, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Protein family/group databases

TCDBi3.A.18.1.1. the nuclear mrna exporter (mrna-e) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Pinin
Alternative name(s):
140 kDa nuclear and cell adhesion-related phosphoprotein
Desmosome-associated protein
Domain-rich serine protein
Short name:
DRS protein
Short name:
DRSP
Melanoma metastasis clone A protein
Nuclear protein SDK3
SR-like protein
Gene namesi
Name:PNN
Synonyms:DRS, MEMA
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 14

Organism-specific databases

HGNCiHGNC:9162. PNN.

Subcellular locationi

GO - Cellular componenti

  • catalytic step 2 spliceosome Source: UniProtKB
  • cell-cell junction Source: ProtInc
  • desmosome Source: UniProtKB-SubCell
  • intermediate filament Source: ProtInc
  • membrane Source: UniProtKB
  • nuclear speck Source: UniProtKB-SubCell
  • nucleoplasm Source: HPA
  • plasma membrane Source: ProtInc
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Nucleus, Spliceosome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi8 – 81L → P: Abolishes interaction with KRT18. 1 Publication
Mutagenesisi19 – 191L → P: Abolishes interaction with KRT18. 1 Publication
Mutagenesisi502 – 5032PE → AA: Abolishes interaction with CTBP1 and shows moderate relief of CTBP1-mediated repression. 1 Publication

Keywords - Diseasei

Tumor suppressor

Organism-specific databases

PharmGKBiPA33484.

Polymorphism and mutation databases

BioMutaiPNN.
DMDMi73921750.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedCombined sources1 Publication
Chaini2 – 717716PininPRO_0000190242Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineCombined sources1 Publication
Modified residuei48 – 481PhosphoserineCombined sources
Modified residuei58 – 581PhosphoserineCombined sources
Modified residuei66 – 661PhosphoserineCombined sources
Modified residuei96 – 961PhosphoserineCombined sources
Modified residuei100 – 1001PhosphoserineCombined sources
Cross-linki109 – 109Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei114 – 1141PhosphoserineCombined sources
Modified residuei115 – 1151PhosphoserineCombined sources
Modified residuei124 – 1241PhosphothreonineCombined sources
Cross-linki157 – 157Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1)Combined sources
Cross-linki157 – 157Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei238 – 2381N6-acetyllysine; alternateCombined sources
Modified residuei238 – 2381N6-succinyllysine; alternateBy similarity
Cross-linki304 – 304Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei347 – 3471PhosphoserineCombined sources
Modified residuei375 – 3751PhosphoserineCombined sources
Modified residuei381 – 3811PhosphoserineCombined sources
Modified residuei441 – 4411PhosphoserineCombined sources
Modified residuei443 – 4431PhosphoserineCombined sources
Modified residuei450 – 4501PhosphoserineCombined sources
Cross-linki528 – 528Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei552 – 5521PhosphoserineCombined sources
Cross-linki553 – 553Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei658 – 6581PhosphoserineCombined sources
Modified residuei692 – 6921PhosphoserineCombined sources
Modified residuei695 – 6951PhosphoserineCombined sources

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ9H307.
MaxQBiQ9H307.
PaxDbiQ9H307.
PeptideAtlasiQ9H307.
PRIDEiQ9H307.

PTM databases

iPTMnetiQ9H307.
PhosphoSiteiQ9H307.
SwissPalmiQ9H307.

Expressioni

Tissue specificityi

Expressed in placenta, lung, liver, kidney, pancreas, spleen, thymus, prostate, testis, ovary, small intestine, colon, heart, epidermis, esophagus, brain and smooth and skeletal muscle. Expressed strongly in melanoma metastasis lesions and advanced primary tumors.3 Publications

Gene expression databases

BgeeiENSG00000100941.
CleanExiHS_PNN.
ExpressionAtlasiQ9H307. baseline and differential.
GenevisibleiQ9H307. HS.

Organism-specific databases

HPAiHPA001378.

Interactioni

Subunit structurei

Found in a mRNA splicing-dependent exon junction complex (EJC). Found in a complex with SR proteins. Found in a mRNP complex with RNPS1. Component of the PSAP complex consisting of RNPS1, SAP18 and PNN. Interacts with PNISR, CTBP1, CTBP2, KRT8, KRT18, KRT19, PS1D/PNO40, PPIG, RNPS1, SFRS4 and SRRM2. Identified in the spliceosome C complex.10 Publications

Protein-protein interaction databases

BioGridi111412. 105 interactions.
DIPiDIP-32950N.
IntActiQ9H307. 31 interactions.
MINTiMINT-1683388.
STRINGi9606.ENSP00000216832.

Structurei

3D structure databases

ProteinModelPortaliQ9H307.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni2 – 284283Necessary for interaction with RNPS1Add
BLAST
Regioni2 – 167166Necessary for mediating alternative 5' splicingAdd
BLAST
Regioni2 – 9897Necessary for interactions with KRT8, KRT18 and KRT19Add
BLAST
Regioni221 – 28464Sufficient for PSAP complex assemblyAdd
BLAST
Regioni606 – 717112Necessary for interaction with PPIGAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili2 – 3231Sequence analysisAdd
BLAST
Coiled coili163 – 23472Sequence analysisAdd
BLAST
Coiled coili287 – 37993Sequence analysisAdd
BLAST
Coiled coili446 – 46722Sequence analysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi172 – 471300Glu-richAdd
BLAST
Compositional biasi469 – 52052Gln-richAdd
BLAST
Compositional biasi552 – 704153Ser-richAdd
BLAST
Compositional biasi632 – 71786Arg-richAdd
BLAST

Sequence similaritiesi

Belongs to the pinin family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG3756. Eukaryota.
ENOG4110W63. LUCA.
HOVERGENiHBG053104.
InParanoidiQ9H307.
KOiK13114.
OrthoDBiEOG091G0S2R.
PhylomeDBiQ9H307.
TreeFamiTF331859.

Family and domain databases

InterProiIPR006786. Pinin_SDK_MemA.
IPR006787. Pinin_SDK_N.
[Graphical view]
PfamiPF04696. Pinin_SDK_memA. 1 hit.
PF04697. Pinin_SDK_N. 1 hit.
[Graphical view]
ProDomiPD011048. Pinin_SDK_N. 1 hit.
[Graphical view] [Entries sharing at least one domain]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9H307-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAVAVRTLQE QLEKAKESLK NVDENIRKLT GRDPNDVRPI QARLLALSGP
60 70 80 90 100
GGGRGRGSLL LRRGFSDSGG GPPAKQRDLE GAVSRLGGER RTRRESRQES
110 120 130 140 150
DPEDDDVKKP ALQSSVVATS KERTRRDLIQ DQNMDEKGKQ RNRRIFGLLM
160 170 180 190 200
GTLQKFKQES TVATERQKRR QEIEQKLEVQ AEEERKQVEN ERRELFEERR
210 220 230 240 250
AKQTELRLLE QKVELAQLQE EWNEHNAKII KYIRTKTKPH LFYIPGRMCP
260 270 280 290 300
ATQKLIEESQ RKMNALFEGR RIEFAEQINK MEARPRRQSM KEKEHQVVRN
310 320 330 340 350
EEQKAEQEEG KVAQREEELE ETGNQHNDVE IEEAGEEEEK EIAIVHSDAE
360 370 380 390 400
KEQEEEEQKQ EMEVKMEEET EVRESEKQQD SQPEEVMDVL EMVENVKHVI
410 420 430 440 450
ADQEVMETNR VESVEPSENE ASKELEPEME FEIEPDKECK SLSPGKENVS
460 470 480 490 500
ALDMEKESEE KEEKESEPQP EPVAQPQPQS QPQLQLQSQS QPVLQSQPPS
510 520 530 540 550
QPEDLSLAVL QPTPQVTQEQ GHLLPERKDF PVESVKLTEV PVEPVLTVHP
560 570 580 590 600
ESKSKTKTRS RSRGRARNKT SKSRSRSSSS SSSSSSSTSS SSGSSSSSGS
610 620 630 640 650
SSSRSSSSSS SSTSGSSSRD SSSSTSSSSE SRSRSRGRGH NRDRKHRRSV
660 670 680 690 700
DRKRRDTSGL ERSHKSSKGG SSRDTKGSKD KNSRSDRKRS ISESSRSGKR
710
SSRSERDRKS DRKDKRR
Length:717
Mass (Da):81,614
Last modified:January 23, 2007 - v4
Checksum:i1C05E1E12F54299F
GO
Isoform 2 (identifier: Q9H307-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-133: Missing.

Show »
Length:584
Mass (Da):67,005
Checksum:i7D96168629D5C4DA
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti69 – 691Missing in AAB48304 (PubMed:8922384).Curated
Sequence conflicti168 – 1681K → N in AAB48304 (PubMed:8922384).Curated
Sequence conflicti268 – 2681E → D in AAB48304 (PubMed:8922384).Curated
Sequence conflicti303 – 3031Q → H in AAB48304 (PubMed:8922384).Curated
Sequence conflicti320 – 3201E → V in AAB48304 (PubMed:8922384).Curated
Sequence conflicti343 – 3431A → G in AAB48304 (PubMed:8922384).Curated
Sequence conflicti402 – 4032DQ → EE in AAG33941 (PubMed:10645008).Curated
Sequence conflicti459 – 4591E → D in AAB48304 (PubMed:8922384).Curated
Sequence conflicti478 – 4781P → A in AAB48304 (PubMed:8922384).Curated
Sequence conflicti491 – 4922QP → EPQPQLQPEPAQPQLQSQPQ LQLQSQCHA AA sequence (PubMed:8922384).Curated
Sequence conflicti497 – 4971Q → H in AAB48304 (PubMed:8922384).Curated
Sequence conflicti520 – 5201Q → H in AAB48304 (PubMed:8922384).Curated
Sequence conflicti523 – 5231L → F in AAB48304 (PubMed:8922384).Curated
Sequence conflicti541 – 5411P → T AA sequence (PubMed:8922384).Curated
Sequence conflicti543 – 5431E → D in AAB48304 (PubMed:8922384).Curated
Sequence conflicti547 – 5471T → I AA sequence (PubMed:8922384).Curated
Sequence conflicti550 – 5501P → S AA sequence (PubMed:8922384).Curated
Sequence conflicti551 – 5511E → D AA sequence (PubMed:8922384).Curated
Sequence conflicti553 – 5575KSKTK → ESETN in AAB48304 (PubMed:8922384).Curated
Sequence conflicti566 – 5661A → T in AAB48304 (PubMed:8922384).Curated
Sequence conflicti569 – 5713KTS → RTT in AAB48304 (PubMed:8922384).Curated
Sequence conflicti618 – 6181S → G in AAF17209 (PubMed:10931946).Curated
Sequence conflicti618 – 6181S → G in CAA71377 (PubMed:9447706).Curated
Sequence conflicti626 – 6261S → STS in CAA70874 (PubMed:10095061).Curated
Sequence conflicti664 – 6641H → P in AAG33941 (PubMed:10645008).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti441 – 4411S → T.
Corresponds to variant rs2180792 [ dbSNP | Ensembl ].
VAR_050540
Natural varianti671 – 6711S → G.2 Publications
Corresponds to variant rs13021 [ dbSNP | Ensembl ].
VAR_023368

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 133133Missing in isoform 2. 2 PublicationsVSP_015307Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U77718 mRNA. Translation: AAB48304.1.
Y09703 mRNA. Translation: CAA70874.1.
AF195139 Genomic DNA. Translation: AAG33941.1.
AF112222 mRNA. Translation: AAF17209.1.
AK303136 mRNA. Translation: BAG64240.1.
AK223612 mRNA. Translation: BAD97332.1.
BC062602 mRNA. Translation: AAH62602.1.
Y10351 mRNA. Translation: CAA71377.1.
CCDSiCCDS9671.1. [Q9H307-1]
RefSeqiNP_002678.2. NM_002687.3. [Q9H307-1]
UniGeneiHs.409965.

Genome annotation databases

EnsembliENST00000216832; ENSP00000216832; ENSG00000100941.
GeneIDi5411.
KEGGihsa:5411.
UCSCiuc001wuw.5. human. [Q9H307-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U77718 mRNA. Translation: AAB48304.1.
Y09703 mRNA. Translation: CAA70874.1.
AF195139 Genomic DNA. Translation: AAG33941.1.
AF112222 mRNA. Translation: AAF17209.1.
AK303136 mRNA. Translation: BAG64240.1.
AK223612 mRNA. Translation: BAD97332.1.
BC062602 mRNA. Translation: AAH62602.1.
Y10351 mRNA. Translation: CAA71377.1.
CCDSiCCDS9671.1. [Q9H307-1]
RefSeqiNP_002678.2. NM_002687.3. [Q9H307-1]
UniGeneiHs.409965.

3D structure databases

ProteinModelPortaliQ9H307.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi111412. 105 interactions.
DIPiDIP-32950N.
IntActiQ9H307. 31 interactions.
MINTiMINT-1683388.
STRINGi9606.ENSP00000216832.

Protein family/group databases

TCDBi3.A.18.1.1. the nuclear mrna exporter (mrna-e) family.

PTM databases

iPTMnetiQ9H307.
PhosphoSiteiQ9H307.
SwissPalmiQ9H307.

Polymorphism and mutation databases

BioMutaiPNN.
DMDMi73921750.

Proteomic databases

EPDiQ9H307.
MaxQBiQ9H307.
PaxDbiQ9H307.
PeptideAtlasiQ9H307.
PRIDEiQ9H307.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000216832; ENSP00000216832; ENSG00000100941.
GeneIDi5411.
KEGGihsa:5411.
UCSCiuc001wuw.5. human. [Q9H307-1]

Organism-specific databases

CTDi5411.
GeneCardsiPNN.
H-InvDBHIX0037750.
HGNCiHGNC:9162. PNN.
HPAiHPA001378.
MIMi603154. gene.
neXtProtiNX_Q9H307.
PharmGKBiPA33484.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3756. Eukaryota.
ENOG4110W63. LUCA.
HOVERGENiHBG053104.
InParanoidiQ9H307.
KOiK13114.
OrthoDBiEOG091G0S2R.
PhylomeDBiQ9H307.
TreeFamiTF331859.

Miscellaneous databases

ChiTaRSiPNN. human.
GeneWikiiPinin.
GenomeRNAii5411.
PROiQ9H307.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000100941.
CleanExiHS_PNN.
ExpressionAtlasiQ9H307. baseline and differential.
GenevisibleiQ9H307. HS.

Family and domain databases

InterProiIPR006786. Pinin_SDK_MemA.
IPR006787. Pinin_SDK_N.
[Graphical view]
PfamiPF04696. Pinin_SDK_memA. 1 hit.
PF04697. Pinin_SDK_N. 1 hit.
[Graphical view]
ProDomiPD011048. Pinin_SDK_N. 1 hit.
[Graphical view] [Entries sharing at least one domain]
ProtoNetiSearch...

Entry informationi

Entry nameiPININ_HUMAN
AccessioniPrimary (citable) accession number: Q9H307
Secondary accession number(s): B4DZX8
, O60899, Q53EM7, Q6P5X4, Q7KYL1, Q99738, Q9UHZ9, Q9UQR9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 30, 2005
Last sequence update: January 23, 2007
Last modified: September 7, 2016
This is version 132 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 14
    Human chromosome 14: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.