Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Solute carrier organic anion transporter family member 5A1

Gene

SLCO5A1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Transport

Protein family/group databases

MEROPSiI01.972.
TCDBi2.A.60.1.16. the organo anion transporter (oat) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Solute carrier organic anion transporter family member 5A1
Alternative name(s):
Organic anion transporter polypeptide-related protein 4
Short name:
OATP-RP4
Short name:
OATPRP4
Solute carrier family 21 member 15
Gene namesi
Name:SLCO5A1
Synonyms:OATP5A1, SLC21A15
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 8

Organism-specific databases

HGNCiHGNC:19046. SLCO5A1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 128128CytoplasmicSequence analysisAdd
BLAST
Transmembranei129 – 14921Helical; Name=1Sequence analysisAdd
BLAST
Topological domaini150 – 16819ExtracellularSequence analysisAdd
BLAST
Transmembranei169 – 18921Helical; Name=2Sequence analysisAdd
BLAST
Topological domaini190 – 1956CytoplasmicSequence analysis
Transmembranei196 – 22025Helical; Name=3Sequence analysisAdd
BLAST
Topological domaini221 – 26040ExtracellularSequence analysisAdd
BLAST
Transmembranei261 – 29030Helical; Name=4Sequence analysisAdd
BLAST
Topological domaini291 – 30919CytoplasmicSequence analysisAdd
BLAST
Transmembranei310 – 32920Helical; Name=5Sequence analysisAdd
BLAST
Topological domaini330 – 34415ExtracellularSequence analysisAdd
BLAST
Transmembranei345 – 36925Helical; Name=6Sequence analysisAdd
BLAST
Topological domaini370 – 42758CytoplasmicSequence analysisAdd
BLAST
Transmembranei428 – 44922Helical; Name=7Sequence analysisAdd
BLAST
Topological domaini450 – 46920ExtracellularSequence analysisAdd
BLAST
Transmembranei470 – 49324Helical; Name=8Sequence analysisAdd
BLAST
Topological domaini494 – 4974CytoplasmicSequence analysis
Transmembranei498 – 52124Helical; Name=9Sequence analysisAdd
BLAST
Topological domaini522 – 651130ExtracellularSequence analysisAdd
BLAST
Transmembranei652 – 67423Helical; Name=10Sequence analysisAdd
BLAST
Topological domaini675 – 6839CytoplasmicSequence analysis
Transmembranei684 – 70926Helical; Name=11Sequence analysisAdd
BLAST
Topological domaini710 – 74233ExtracellularSequence analysisAdd
BLAST
Transmembranei743 – 76018Helical; Name=12Sequence analysisAdd
BLAST
Topological domaini761 – 80848CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134948571.

Polymorphism and mutation databases

BioMutaiSLCO5A1.
DMDMi296452911.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 848848Solute carrier organic anion transporter family member 5A1PRO_0000191070Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi228 – 2281N-linked (GlcNAc...)Sequence analysis
Glycosylationi241 – 2411N-linked (GlcNAc...)Sequence analysis
Glycosylationi469 – 4691N-linked (GlcNAc...)Sequence analysis
Glycosylationi550 – 5501N-linked (GlcNAc...)Sequence analysis
Disulfide bondi555 ↔ 581PROSITE-ProRule annotation
Disulfide bondi559 ↔ 570PROSITE-ProRule annotation
Disulfide bondi561 ↔ 585PROSITE-ProRule annotation
Glycosylationi590 – 5901N-linked (GlcNAc...)Sequence analysis
Glycosylationi597 – 5971N-linked (GlcNAc...)Sequence analysis
Glycosylationi730 – 7301N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ9H2Y9.
PRIDEiQ9H2Y9.

PTM databases

iPTMnetiQ9H2Y9.
PhosphoSiteiQ9H2Y9.

Expressioni

Gene expression databases

BgeeiQ9H2Y9.
CleanExiHS_SLCO5A1.
ExpressionAtlasiQ9H2Y9. baseline and differential.
GenevisibleiQ9H2Y9. HS.

Organism-specific databases

HPAiHPA025062.

Interactioni

Protein-protein interaction databases

IntActiQ9H2Y9. 1 interaction.
STRINGi9606.ENSP00000260126.

Structurei

3D structure databases

ProteinModelPortaliQ9H2Y9.
SMRiQ9H2Y9. Positions 553-585.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini549 – 60355Kazal-likePROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi377 – 3837Poly-Lys

Sequence similaritiesi

Contains 1 Kazal-like domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3626. Eukaryota.
ENOG410XRSF. LUCA.
GeneTreeiENSGT00760000119014.
HOGENOMiHOG000060097.
HOVERGENiHBG069241.
InParanoidiQ9H2Y9.
KOiK14356.
OMAiCFDIGNL.
OrthoDBiEOG75TMBK.
PhylomeDBiQ9H2Y9.
TreeFamiTF317540.

Family and domain databases

InterProiIPR002350. Kazal_dom.
IPR020846. MFS_dom.
IPR004156. OA_transporter.
[Graphical view]
PANTHERiPTHR11388. PTHR11388. 2 hits.
PfamiPF07648. Kazal_2. 1 hit.
PF03137. OATP. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 4 hits.
TIGRFAMsiTIGR00805. oat. 1 hit.
PROSITEiPS51465. KAZAL_2. 1 hit.
PS50850. MFS. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9H2Y9-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDEGTGLQPG AGEQLEAPAT AEAVQERCEP ETLRSKSLPV LSSASCRPSL
60 70 80 90 100
SPTSGDANPA FGCVDSSGHQ ELKQGPNPLA PSPSAPSTSA GLGDCNHRVD
110 120 130 140 150
LSKTFSVSSA LAMLQERRCL YVVLTDSRCF LVCMCFLTFI QALMVSGYLS
160 170 180 190 200
SVITTIERRY SLKSSESGLL VSCFDIGNLV VVVFVSYFGG RGRRPLWLAV
210 220 230 240 250
GGLLIAFGAA LFALPHFISP PYQIQELNAS APNDGLCQGG NSTATLEPPA
260 270 280 290 300
CPKDSGGNNH WVYVALFICA QILIGMGSTP IYTLGPTYLD DNVKKENSSL
310 320 330 340 350
YLAIMYVMGA LGPAVGYLLG GLLIGFYVDP RNPVHLDQND PRFIGNWWSG
360 370 380 390 400
FLLCAIAMFL VIFPMFTFPK KLPPRHKKKK KKKFSVDAVS DDDVLKEKSN
410 420 430 440 450
NSEQADKKVS SMGFGKDVRD LPRAAVRILS NMTFLFVSLS YTAESAIVTA
460 470 480 490 500
FITFIPKFIE SQFGIPASNA SIYTGVIIVP SAGVGIVLGG YIIKKLKLGA
510 520 530 540 550
RESAKLAMIC SGVSLLCFST LFIVGCESIN LGGINIPYTT GPSLTMPHRN
560 570 580 590 600
LTGSCNVNCG CKIHEYEPVC GSDGITYFNP CLAGCVNSGN LSTGIRNYTE
610 620 630 640 650
CTCVQSRQVI TPPTVGQRSQ LRVVIVKTYL NENGYAVSGK CKRTCNTLIP
660 670 680 690 700
FLVFLFIVTF ITACAQPSAI IVTLRSVEDE ERPFALGMQF VLLRTLAYIP
710 720 730 740 750
TPIYFGAVID TTCMLWQQEC GVQGSCWEYN VTSFRFVYFG LAAGLKFVGF
760 770 780 790 800
IFIFLAWYSI KYKEDGLQRR RQREFPLSTV SERVGHPDNA RTRSCPAFST
810 820 830 840
QGEFHEETGL QKGIQCAAQT YPGPFPEAIS SSADPGLEES PAALEPPS
Length:848
Mass (Da):91,864
Last modified:May 18, 2010 - v2
Checksum:iF213B18FE8389596
GO
Isoform 2 (identifier: Q9H2Y9-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     420-474: Missing.

Note: No experimental confirmation available.
Show »
Length:793
Mass (Da):85,850
Checksum:iB1031ACFCC20CCB8
GO
Isoform 3 (identifier: Q9H2Y9-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     675-687: RSVEDEERPFALG → SIHSYSNLLWSSH
     688-848: Missing.

Note: No experimental confirmation available.
Show »
Length:687
Mass (Da):73,843
Checksum:i772B469C80E2BEEE
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti40 – 401V → A in BAG51109 (PubMed:14702039).Curated
Sequence conflicti264 – 2641V → L in AAG42207 (Ref. 1) Curated
Sequence conflicti457 – 4571K → E in BAG51109 (PubMed:14702039).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti33 – 331L → F.1 Publication
Corresponds to variant rs3750266 [ dbSNP | Ensembl ].
VAR_022041

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei420 – 47455Missing in isoform 2. 1 PublicationVSP_045498Add
BLAST
Alternative sequencei675 – 68713RSVED…PFALG → SIHSYSNLLWSSH in isoform 3. 1 PublicationVSP_045499Add
BLAST
Alternative sequencei688 – 848161Missing in isoform 3. 1 PublicationVSP_045500Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF205075 mRNA. Translation: AAG42207.1.
AK022760 mRNA. Translation: BAG51109.1.
AC024680 Genomic DNA. No translation available.
AC079089 Genomic DNA. No translation available.
AC091047 Genomic DNA. No translation available.
CH471068 Genomic DNA. Translation: EAW86957.1.
CH471068 Genomic DNA. Translation: EAW86958.1.
BC137424 mRNA. Translation: AAI37425.1.
BC139755 mRNA. Translation: AAI39756.1.
CCDSiCCDS55242.1. [Q9H2Y9-2]
CCDS55243.1. [Q9H2Y9-3]
CCDS6205.1. [Q9H2Y9-1]
RefSeqiNP_001139480.1. NM_001146008.1. [Q9H2Y9-3]
NP_001139481.1. NM_001146009.1. [Q9H2Y9-2]
NP_112220.2. NM_030958.2. [Q9H2Y9-1]
XP_005251370.1. XM_005251313.2. [Q9H2Y9-2]
UniGeneiHs.152460.
Hs.443609.

Genome annotation databases

EnsembliENST00000260126; ENSP00000260126; ENSG00000137571. [Q9H2Y9-1]
ENST00000524945; ENSP00000434422; ENSG00000137571. [Q9H2Y9-3]
ENST00000530307; ENSP00000431611; ENSG00000137571. [Q9H2Y9-2]
GeneIDi81796.
KEGGihsa:81796.
UCSCiuc003xyk.4. human. [Q9H2Y9-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF205075 mRNA. Translation: AAG42207.1.
AK022760 mRNA. Translation: BAG51109.1.
AC024680 Genomic DNA. No translation available.
AC079089 Genomic DNA. No translation available.
AC091047 Genomic DNA. No translation available.
CH471068 Genomic DNA. Translation: EAW86957.1.
CH471068 Genomic DNA. Translation: EAW86958.1.
BC137424 mRNA. Translation: AAI37425.1.
BC139755 mRNA. Translation: AAI39756.1.
CCDSiCCDS55242.1. [Q9H2Y9-2]
CCDS55243.1. [Q9H2Y9-3]
CCDS6205.1. [Q9H2Y9-1]
RefSeqiNP_001139480.1. NM_001146008.1. [Q9H2Y9-3]
NP_001139481.1. NM_001146009.1. [Q9H2Y9-2]
NP_112220.2. NM_030958.2. [Q9H2Y9-1]
XP_005251370.1. XM_005251313.2. [Q9H2Y9-2]
UniGeneiHs.152460.
Hs.443609.

3D structure databases

ProteinModelPortaliQ9H2Y9.
SMRiQ9H2Y9. Positions 553-585.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9H2Y9. 1 interaction.
STRINGi9606.ENSP00000260126.

Protein family/group databases

MEROPSiI01.972.
TCDBi2.A.60.1.16. the organo anion transporter (oat) family.

PTM databases

iPTMnetiQ9H2Y9.
PhosphoSiteiQ9H2Y9.

Polymorphism and mutation databases

BioMutaiSLCO5A1.
DMDMi296452911.

Proteomic databases

PaxDbiQ9H2Y9.
PRIDEiQ9H2Y9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000260126; ENSP00000260126; ENSG00000137571. [Q9H2Y9-1]
ENST00000524945; ENSP00000434422; ENSG00000137571. [Q9H2Y9-3]
ENST00000530307; ENSP00000431611; ENSG00000137571. [Q9H2Y9-2]
GeneIDi81796.
KEGGihsa:81796.
UCSCiuc003xyk.4. human. [Q9H2Y9-1]

Organism-specific databases

CTDi81796.
GeneCardsiSLCO5A1.
HGNCiHGNC:19046. SLCO5A1.
HPAiHPA025062.
MIMi613543. gene.
neXtProtiNX_Q9H2Y9.
PharmGKBiPA134948571.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3626. Eukaryota.
ENOG410XRSF. LUCA.
GeneTreeiENSGT00760000119014.
HOGENOMiHOG000060097.
HOVERGENiHBG069241.
InParanoidiQ9H2Y9.
KOiK14356.
OMAiCFDIGNL.
OrthoDBiEOG75TMBK.
PhylomeDBiQ9H2Y9.
TreeFamiTF317540.

Miscellaneous databases

GenomeRNAii81796.
NextBioi72114.
PROiQ9H2Y9.
SOURCEiSearch...

Gene expression databases

BgeeiQ9H2Y9.
CleanExiHS_SLCO5A1.
ExpressionAtlasiQ9H2Y9. baseline and differential.
GenevisibleiQ9H2Y9. HS.

Family and domain databases

InterProiIPR002350. Kazal_dom.
IPR020846. MFS_dom.
IPR004156. OA_transporter.
[Graphical view]
PANTHERiPTHR11388. PTHR11388. 2 hits.
PfamiPF07648. Kazal_2. 1 hit.
PF03137. OATP. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 4 hits.
TIGRFAMsiTIGR00805. oat. 1 hit.
PROSITEiPS51465. KAZAL_2. 1 hit.
PS50850. MFS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification and characterization of novel human OATP family members."
    Wu Y., Hsiang B.H., Zhu Y., Yang W.-P., Kirchgessner T.G.
    Submitted (NOV-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
  3. "DNA sequence and analysis of human chromosome 8."
    Nusbaum C., Mikkelsen T.S., Zody M.C., Asakawa S., Taudien S., Garber M., Kodira C.D., Schueler M.G., Shimizu A., Whittaker C.A., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Yang X., Allen N.R., Anderson S., Asakawa T.
    , Blechschmidt K., Bloom T., Borowsky M.L., Butler J., Cook A., Corum B., DeArellano K., DeCaprio D., Dooley K.T., Dorris L. III, Engels R., Gloeckner G., Hafez N., Hagopian D.S., Hall J.L., Ishikawa S.K., Jaffe D.B., Kamat A., Kudoh J., Lehmann R., Lokitsang T., Macdonald P., Major J.E., Matthews C.D., Mauceli E., Menzel U., Mihalev A.H., Minoshima S., Murayama Y., Naylor J.W., Nicol R., Nguyen C., O'Leary S.B., O'Neill K., Parker S.C.J., Polley A., Raymond C.K., Reichwald K., Rodriguez J., Sasaki T., Schilhabel M., Siddiqui R., Smith C.L., Sneddon T.P., Talamas J.A., Tenzin P., Topham K., Venkataraman V., Wen G., Yamazaki S., Young S.K., Zeng Q., Zimmer A.R., Rosenthal A., Birren B.W., Platzer M., Shimizu N., Lander E.S.
    Nature 439:331-335(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2), VARIANT PHE-33.

Entry informationi

Entry nameiSO5A1_HUMAN
AccessioniPrimary (citable) accession number: Q9H2Y9
Secondary accession number(s): A4QPC2
, B2RPF7, B3KMU7, E9PKK5, G3V1C0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 10, 2003
Last sequence update: May 18, 2010
Last modified: May 11, 2016
This is version 128 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 8
    Human chromosome 8: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.