Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q9H2X0

- CHRD_HUMAN

UniProt

Q9H2X0 - CHRD_HUMAN

Protein

Chordin

Gene

CHRD

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 112 (01 Oct 2014)
      Sequence version 2 (28 Nov 2006)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Dorsalizing factor. Key developmental protein that dorsalizes early vertebrate embryonic tissues by binding to ventralizing TGF-beta family bone morphogenetic proteins (BMPs) and sequestering them in latent complexes By similarity.By similarity

    GO - Molecular functioni

    1. cytokine binding Source: BHF-UCL
    2. heparin binding Source: Ensembl
    3. protein binding Source: IntAct

    GO - Biological processi

    1. BMP signaling pathway involved in spinal cord dorsal/ventral patterning Source: BHF-UCL
    2. floor plate development Source: BHF-UCL
    3. forebrain development Source: Ensembl
    4. gastrulation with mouth forming second Source: Ensembl
    5. mesoderm formation Source: Ensembl
    6. negative regulation of BMP signaling pathway Source: BHF-UCL
    7. negative regulation of cell migration Source: BHF-UCL
    8. negative regulation of osteoblast differentiation Source: BHF-UCL
    9. osteoblast differentiation Source: Ensembl
    10. positive regulation of cell adhesion Source: BHF-UCL
    11. positive regulation of mesenchymal cell proliferation Source: BHF-UCL
    12. skeletal system development Source: UniProtKB

    Keywords - Molecular functioni

    Developmental protein

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Chordin
    Gene namesi
    Name:CHRD
    ORF Names:UNQ217/PRO243
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 3

    Organism-specific databases

    HGNCiHGNC:1949. CHRD.

    Subcellular locationi

    Secreted By similarity

    GO - Cellular componenti

    1. extracellular space Source: BHF-UCL

    Keywords - Cellular componenti

    Secreted

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA26482.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2626Sequence AnalysisAdd
    BLAST
    Chaini27 – 955929ChordinPRO_0000005364Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi217 – 2171N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi351 – 3511N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi365 – 3651N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi434 – 4341N-linked (GlcNAc...)Sequence Analysis

    Post-translational modificationi

    Cleaved by tolloid proteases; cleavage participates in dorsoventral patterning during early development.By similarity

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PaxDbiQ9H2X0.
    PRIDEiQ9H2X0.

    PTM databases

    PhosphoSiteiQ9H2X0.

    Miscellaneous databases

    PMAP-CutDBQ9H2X0.

    Expressioni

    Tissue specificityi

    Expressed at the highest level in liver.

    Gene expression databases

    ArrayExpressiQ9H2X0.
    BgeeiQ9H2X0.
    GenevestigatoriQ9H2X0.

    Organism-specific databases

    HPAiCAB020705.
    HPA035827.

    Interactioni

    Subunit structurei

    Interacts with TWSG1 and/or BMP4.By similarity

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    SMAD3P840222EBI-947551,EBI-347161

    Protein-protein interaction databases

    BioGridi114198. 8 interactions.
    DIPiDIP-48857N.
    IntActiQ9H2X0. 11 interactions.
    MINTiMINT-2817631.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9H2X0.
    SMRiQ9H2X0. Positions 46-131, 700-766, 780-855, 867-937.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini49 – 12678VWFC 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini168 – 277110CHRD 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini279 – 402124CHRD 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini403 – 524122CHRD 3PROSITE-ProRule annotationAdd
    BLAST
    Domaini530 – 650121CHRD 4PROSITE-ProRule annotationAdd
    BLAST
    Domaini703 – 76361VWFC 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini784 – 85067VWFC 3PROSITE-ProRule annotationAdd
    BLAST
    Domaini872 – 93261VWFC 4PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the chordin family.Curated
    Contains 4 CHRD domains.PROSITE-ProRule annotation
    Contains 4 VWFC domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat, Signal

    Phylogenomic databases

    eggNOGiNOG85639.
    HOVERGENiHBG005330.
    InParanoidiQ9H2X0.
    KOiK04657.
    OMAiRDPGEGC.
    OrthoDBiEOG7QNVK7.
    PhylomeDBiQ9H2X0.
    TreeFamiTF106451.

    Family and domain databases

    InterProiIPR016353. Chordin.
    IPR010895. CHRD.
    IPR001007. VWF_C.
    [Graphical view]
    PfamiPF07452. CHRD. 4 hits.
    PF00093. VWC. 3 hits.
    [Graphical view]
    PIRSFiPIRSF002496. Chordin. 1 hit.
    SMARTiSM00754. CHRD. 4 hits.
    SM00214. VWC. 4 hits.
    [Graphical view]
    PROSITEiPS50933. CHRD. 4 hits.
    PS01208. VWFC_1. 2 hits.
    PS50184. VWFC_2. 2 hits.
    [Graphical view]

    Sequences (5)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 5 isoformsi produced by alternative splicing. Align

    Note: Experimental confirmation may be lacking for some isoforms.

    Isoform 1 (identifier: Q9H2X0-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MPSLPAPPAP LLLLGLLLLG SRPARGAGPE PPVLPIRSEK EPLPVRGAAG    50
    CTFGGKVYAL DETWHPDLGE PFGVMRCVLC ACEAPQWGRR TRGPGRVSCK 100
    NIKPECPTPA CGQPRQLPGH CCQTCPQERS SSERQPSGLS FEYPRDPEHR 150
    SYSDRGEPGA EERARGDGHT DFVALLTGPR SQAVARARVS LLRSSLRFSI 200
    SYRRLDRPTR IRFSDSNGSV LFEHPAAPTQ DGLVCGVWRA VPRLSLRLLR 250
    AEQLHVALVT LTHPSGEVWG PLIRHRALAA ETFSAILTLE GPPQQGVGGI 300
    TLLTLSDTED SLHFLLLFRG LLEPRSGGLT QVPLRLQILH QGQLLRELQA 350
    NVSAQEPGFA EVLPNLTVQE MDWLVLGELQ MALEWAGRPG LRISGHIAAR 400
    KSCDVLQSVL CGADALIPVQ TGAAGSASLT LLGNGSLIYQ VQVVGTSSEV 450
    VAMTLETKPQ RRDQRTVLCH MAGLQPGGHT AVGICPGLGA RGAHMLLQNE 500
    LFLNVGTKDF PDGELRGHVA ALPYCGHSAR HDTLPVPLAG ALVLPPVKSQ 550
    AAGHAWLSLD THCHLHYEVL LAGLGGSEQG TVTAHLLGPP GTPGPRRLLK 600
    GFYGSEAQGV VKDLEPELLR HLAKGMASLM ITTKGSPRGE LRGQVHIANQ 650
    CEVGGLRLEA AGAEGVRALG APDTASAAPP VVPGLPALAP AKPGGPGRPR 700
    DPNTCFFEGQ QRPHGARWAP NYDPLCSLCT CQRRTVICDP VVCPPPSCPH 750
    PVQAPDQCCP VCPEKQDVRD LPGLPRSRDP GEGCYFDGDR SWRAAGTRWH 800
    PVVPPFGLIK CAVCTCKGGT GEVHCEKVQC PRLACAQPVR VNPTDCCKQC 850
    PVGSGAHPQL GDPMQADGPR GCRFAGQWFP ESQSWHPSVP PFGEMSCITC 900
    RCGAGVPHCE RDDCSLPLSC GSGKESRCCS RCTAHRRPAP ETRTDPELEK 950
    EAEGS 955
    Length:955
    Mass (Da):102,032
    Last modified:November 28, 2006 - v2
    Checksum:i53F9D9F39A517686
    GO
    Isoform 2 (identifier: Q9H2X0-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         85-86: PQ → GP
         87-955: Missing.

    Note: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.

    Show »
    Length:86
    Mass (Da):8,897
    Checksum:i0679D7166DF9F994
    GO
    Isoform 3 (identifier: Q9H2X0-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         85-94: PQWGRRTRGP → TGTLRPREMK
         95-955: Missing.

    Note: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.

    Show »
    Length:94
    Mass (Da):9,913
    Checksum:i74479F0AE711A2C4
    GO
    Isoform 4 (identifier: Q9H2X0-4) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         328-350: GLTQVPLRLQILHQGQLLRELQA → DSTPGAATARTSGQCLSPGTRLC
         351-955: Missing.

    Note: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.

    Show »
    Length:350
    Mass (Da):37,782
    Checksum:iF18D4B0ED3EB6A98
    GO
    Isoform 5 (identifier: Q9H2X0-5) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         441-480: Missing.

    Show »
    Length:915
    Mass (Da):97,731
    Checksum:i506ADC62F09ED062
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti70 – 701E → Q in AAQ89285. (PubMed:12975309)Curated
    Sequence conflicti115 – 1184RQLP → QVAA in AAC69835. (PubMed:9782094)Curated
    Sequence conflicti189 – 1891V → A in AAC69835. (PubMed:9782094)Curated
    Sequence conflicti216 – 2161S → P in AAC69835. (PubMed:9782094)Curated
    Sequence conflicti674 – 6741T → P in AAC69835. (PubMed:9782094)Curated
    Sequence conflicti939 – 9391Missing in AAQ89285. (PubMed:12975309)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti94 – 941P → S.
    Corresponds to variant rs34095724 [ dbSNP | Ensembl ].
    VAR_048727
    Natural varianti630 – 6301M → L.2 Publications
    Corresponds to variant rs16858780 [ dbSNP | Ensembl ].
    VAR_021517

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei85 – 9410PQWGRRTRGP → TGTLRPREMK in isoform 3. 1 PublicationVSP_001071
    Alternative sequencei85 – 862PQ → GP in isoform 2. 1 PublicationVSP_001069
    Alternative sequencei87 – 955869Missing in isoform 2. 1 PublicationVSP_001070Add
    BLAST
    Alternative sequencei95 – 955861Missing in isoform 3. 1 PublicationVSP_001072Add
    BLAST
    Alternative sequencei328 – 35023GLTQV…RELQA → DSTPGAATARTSGQCLSPGT RLC in isoform 4. 1 PublicationVSP_001073Add
    BLAST
    Alternative sequencei351 – 955605Missing in isoform 4. 1 PublicationVSP_001074Add
    BLAST
    Alternative sequencei441 – 48040Missing in isoform 5. 1 PublicationVSP_001075Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF209928 mRNA. Translation: AAG35767.1.
    AF209929 mRNA. Translation: AAG35768.1.
    AF209930 mRNA. Translation: AAG35769.1.
    AF283325 mRNA. Translation: AAG35784.1.
    AY358926 mRNA. Translation: AAQ89285.1.
    BC112345 mRNA. Translation: AAI12346.1.
    AF076612 mRNA. Translation: AAC69835.1.
    AF136632 Genomic DNA. Translation: AAF70236.1.
    AF136633 Genomic DNA. Translation: AAF70237.1.
    AF136634 Genomic DNA. Translation: AAF70238.1.
    AF136635 Genomic DNA. Translation: AAF70239.1.
    CCDSiCCDS3266.1. [Q9H2X0-1]
    RefSeqiNP_003732.2. NM_003741.2. [Q9H2X0-1]
    UniGeneiHs.166186.

    Genome annotation databases

    EnsembliENST00000204604; ENSP00000204604; ENSG00000090539. [Q9H2X0-1]
    ENST00000348986; ENSP00000334036; ENSG00000090539. [Q9H2X0-5]
    ENST00000356534; ENSP00000348930; ENSG00000090539. [Q9H2X0-2]
    ENST00000420973; ENSP00000392794; ENSG00000090539. [Q9H2X0-4]
    ENST00000448472; ENSP00000408624; ENSG00000090539. [Q9H2X0-3]
    GeneIDi8646.
    KEGGihsa:8646.
    UCSCiuc003fov.3. human. [Q9H2X0-1]
    uc010hyc.3. human. [Q9H2X0-5]

    Polymorphism databases

    DMDMi118572631.

    Keywords - Coding sequence diversityi

    Alternative splicing, Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF209928 mRNA. Translation: AAG35767.1 .
    AF209929 mRNA. Translation: AAG35768.1 .
    AF209930 mRNA. Translation: AAG35769.1 .
    AF283325 mRNA. Translation: AAG35784.1 .
    AY358926 mRNA. Translation: AAQ89285.1 .
    BC112345 mRNA. Translation: AAI12346.1 .
    AF076612 mRNA. Translation: AAC69835.1 .
    AF136632 Genomic DNA. Translation: AAF70236.1 .
    AF136633 Genomic DNA. Translation: AAF70237.1 .
    AF136634 Genomic DNA. Translation: AAF70238.1 .
    AF136635 Genomic DNA. Translation: AAF70239.1 .
    CCDSi CCDS3266.1. [Q9H2X0-1 ]
    RefSeqi NP_003732.2. NM_003741.2. [Q9H2X0-1 ]
    UniGenei Hs.166186.

    3D structure databases

    ProteinModelPortali Q9H2X0.
    SMRi Q9H2X0. Positions 46-131, 700-766, 780-855, 867-937.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 114198. 8 interactions.
    DIPi DIP-48857N.
    IntActi Q9H2X0. 11 interactions.
    MINTi MINT-2817631.

    PTM databases

    PhosphoSitei Q9H2X0.

    Polymorphism databases

    DMDMi 118572631.

    Proteomic databases

    PaxDbi Q9H2X0.
    PRIDEi Q9H2X0.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000204604 ; ENSP00000204604 ; ENSG00000090539 . [Q9H2X0-1 ]
    ENST00000348986 ; ENSP00000334036 ; ENSG00000090539 . [Q9H2X0-5 ]
    ENST00000356534 ; ENSP00000348930 ; ENSG00000090539 . [Q9H2X0-2 ]
    ENST00000420973 ; ENSP00000392794 ; ENSG00000090539 . [Q9H2X0-4 ]
    ENST00000448472 ; ENSP00000408624 ; ENSG00000090539 . [Q9H2X0-3 ]
    GeneIDi 8646.
    KEGGi hsa:8646.
    UCSCi uc003fov.3. human. [Q9H2X0-1 ]
    uc010hyc.3. human. [Q9H2X0-5 ]

    Organism-specific databases

    CTDi 8646.
    GeneCardsi GC03P184097.
    H-InvDB HIX0003911.
    HGNCi HGNC:1949. CHRD.
    HPAi CAB020705.
    HPA035827.
    MIMi 603475. gene.
    neXtProti NX_Q9H2X0.
    PharmGKBi PA26482.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG85639.
    HOVERGENi HBG005330.
    InParanoidi Q9H2X0.
    KOi K04657.
    OMAi RDPGEGC.
    OrthoDBi EOG7QNVK7.
    PhylomeDBi Q9H2X0.
    TreeFami TF106451.

    Miscellaneous databases

    GenomeRNAii 8646.
    NextBioi 32415.
    PMAP-CutDB Q9H2X0.
    PROi Q9H2X0.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q9H2X0.
    Bgeei Q9H2X0.
    Genevestigatori Q9H2X0.

    Family and domain databases

    InterProi IPR016353. Chordin.
    IPR010895. CHRD.
    IPR001007. VWF_C.
    [Graphical view ]
    Pfami PF07452. CHRD. 4 hits.
    PF00093. VWC. 3 hits.
    [Graphical view ]
    PIRSFi PIRSF002496. Chordin. 1 hit.
    SMARTi SM00754. CHRD. 4 hits.
    SM00214. VWC. 4 hits.
    [Graphical view ]
    PROSITEi PS50933. CHRD. 4 hits.
    PS01208. VWFC_1. 2 hits.
    PS50184. VWFC_2. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The human chordin gene encodes several differentially expressed spliced variants with distinct BMP opposing activities."
      Millet C., Lemaire P., Orsetti B., Guglielmi P., Francois V.
      Mech. Dev. 106:85-96(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2; 3 AND 4), VARIANT LEU-630.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    4. "Coding sequence and expression patterns of mouse chordin and mapping of the cognate mouse chrd and human CHRD genes."
      Pappano W.N., Scott I.C., Clark T.G., Eddy R.L., Shows T.B., Greenspan D.S.
      Genomics 52:236-239(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 115-955 (ISOFORM 5), VARIANT LEU-630.
    5. "BMP-binding modules in chordin: a model for signalling regulation in the extracellular space."
      Larrain J., Bachiller D., Lu B., Agius E., Piccolo S., De Robertis E.M.
      Development 127:821-830(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 51-125; 705-762; 784-850 AND 872-932.
    6. Cited for: SPLICE ISOFORM(S) THAT ARE POTENTIAL NMD TARGET(S).

    Entry informationi

    Entry nameiCHRD_HUMAN
    AccessioniPrimary (citable) accession number: Q9H2X0
    Secondary accession number(s): O95254
    , Q2M1I8, Q6UW83, Q9H2D3, Q9H2W8, Q9H2W9, Q9P0Z2, Q9P0Z3, Q9P0Z4, Q9P0Z5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 26, 2001
    Last sequence update: November 28, 2006
    Last modified: October 1, 2014
    This is version 112 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 3
      Human chromosome 3: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3