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Q9H2X0 (CHRD_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 111. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Chordin
Gene names
Name:CHRD
ORF Names:UNQ217/PRO243
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length955 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Dorsalizing factor. Key developmental protein that dorsalizes early vertebrate embryonic tissues by binding to ventralizing TGF-beta family bone morphogenetic proteins (BMPs) and sequestering them in latent complexes By similarity.

Subunit structure

Interacts with TWSG1 and/or BMP4 By similarity.

Subcellular location

Secreted By similarity.

Tissue specificity

Expressed at the highest level in liver.

Post-translational modification

Cleaved by tolloid proteases; cleavage participates in dorsoventral patterning during early development By similarity.

Sequence similarities

Belongs to the chordin family.

Contains 4 CHRD domains.

Contains 4 VWFC domains.

Ontologies

Keywords
   Cellular componentSecreted
   Coding sequence diversityAlternative splicing
Polymorphism
   DomainRepeat
Signal
   Molecular functionDevelopmental protein
   PTMGlycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processBMP signaling pathway involved in spinal cord dorsal/ventral patterning

Inferred from mutant phenotype Ref.1. Source: BHF-UCL

floor plate development

Traceable author statement PubMed 11790302. Source: BHF-UCL

forebrain development

Inferred from electronic annotation. Source: Ensembl

gastrulation with mouth forming second

Inferred from electronic annotation. Source: Ensembl

mesoderm formation

Inferred from electronic annotation. Source: Ensembl

negative regulation of BMP signaling pathway

Inferred from mutant phenotype Ref.1. Source: BHF-UCL

negative regulation of cell migration

Inferred from direct assay PubMed 16449796. Source: BHF-UCL

negative regulation of osteoblast differentiation

Inferred from mutant phenotype PubMed 18533030. Source: BHF-UCL

osteoblast differentiation

Inferred from electronic annotation. Source: Ensembl

positive regulation of cell adhesion

Inferred from direct assay PubMed 16449796. Source: BHF-UCL

positive regulation of mesenchymal cell proliferation

Inferred from mutant phenotype PubMed 18533030. Source: BHF-UCL

skeletal system development

Traceable author statement Ref.4. Source: UniProtKB

   Cellular_componentextracellular space

Non-traceable author statement PubMed 16449796. Source: BHF-UCL

   Molecular_functioncytokine binding

Non-traceable author statement PubMed 16449796. Source: BHF-UCL

heparin binding

Inferred from electronic annotation. Source: Ensembl

protein binding

Inferred from physical interaction PubMed 21988832. Source: IntAct

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

SMAD3P840222EBI-947551,EBI-347161

Alternative products

This entry describes 5 isoforms produced by alternative splicing. [Align] [Select]

Note: Experimental confirmation may be lacking for some isoforms.
Isoform 1 (identifier: Q9H2X0-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9H2X0-2)

The sequence of this isoform differs from the canonical sequence as follows:
     85-86: PQ → GP
     87-955: Missing.
Note: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.
Isoform 3 (identifier: Q9H2X0-3)

The sequence of this isoform differs from the canonical sequence as follows:
     85-94: PQWGRRTRGP → TGTLRPREMK
     95-955: Missing.
Note: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.
Isoform 4 (identifier: Q9H2X0-4)

The sequence of this isoform differs from the canonical sequence as follows:
     328-350: GLTQVPLRLQILHQGQLLRELQA → DSTPGAATARTSGQCLSPGTRLC
     351-955: Missing.
Note: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.
Isoform 5 (identifier: Q9H2X0-5)

The sequence of this isoform differs from the canonical sequence as follows:
     441-480: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2626 Potential
Chain27 – 955929Chordin
PRO_0000005364

Regions

Domain49 – 12678VWFC 1
Domain168 – 277110CHRD 1
Domain279 – 402124CHRD 2
Domain403 – 524122CHRD 3
Domain530 – 650121CHRD 4
Domain703 – 76361VWFC 2
Domain784 – 85067VWFC 3
Domain872 – 93261VWFC 4

Amino acid modifications

Glycosylation2171N-linked (GlcNAc...) Potential
Glycosylation3511N-linked (GlcNAc...) Potential
Glycosylation3651N-linked (GlcNAc...) Potential
Glycosylation4341N-linked (GlcNAc...) Potential

Natural variations

Alternative sequence85 – 9410PQWGRRTRGP → TGTLRPREMK in isoform 3.
VSP_001071
Alternative sequence85 – 862PQ → GP in isoform 2.
VSP_001069
Alternative sequence87 – 955869Missing in isoform 2.
VSP_001070
Alternative sequence95 – 955861Missing in isoform 3.
VSP_001072
Alternative sequence328 – 35023GLTQV…RELQA → DSTPGAATARTSGQCLSPGT RLC in isoform 4.
VSP_001073
Alternative sequence351 – 955605Missing in isoform 4.
VSP_001074
Alternative sequence441 – 48040Missing in isoform 5.
VSP_001075
Natural variant941P → S.
Corresponds to variant rs34095724 [ dbSNP | Ensembl ].
VAR_048727
Natural variant6301M → L. Ref.1 Ref.4
Corresponds to variant rs16858780 [ dbSNP | Ensembl ].
VAR_021517

Experimental info

Sequence conflict701E → Q in AAQ89285. Ref.2
Sequence conflict115 – 1184RQLP → QVAA in AAC69835. Ref.4
Sequence conflict1891V → A in AAC69835. Ref.4
Sequence conflict2161S → P in AAC69835. Ref.4
Sequence conflict6741T → P in AAC69835. Ref.4
Sequence conflict9391Missing in AAQ89285. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified November 28, 2006. Version 2.
Checksum: 53F9D9F39A517686

FASTA955102,032
        10         20         30         40         50         60 
MPSLPAPPAP LLLLGLLLLG SRPARGAGPE PPVLPIRSEK EPLPVRGAAG CTFGGKVYAL 

        70         80         90        100        110        120 
DETWHPDLGE PFGVMRCVLC ACEAPQWGRR TRGPGRVSCK NIKPECPTPA CGQPRQLPGH 

       130        140        150        160        170        180 
CCQTCPQERS SSERQPSGLS FEYPRDPEHR SYSDRGEPGA EERARGDGHT DFVALLTGPR 

       190        200        210        220        230        240 
SQAVARARVS LLRSSLRFSI SYRRLDRPTR IRFSDSNGSV LFEHPAAPTQ DGLVCGVWRA 

       250        260        270        280        290        300 
VPRLSLRLLR AEQLHVALVT LTHPSGEVWG PLIRHRALAA ETFSAILTLE GPPQQGVGGI 

       310        320        330        340        350        360 
TLLTLSDTED SLHFLLLFRG LLEPRSGGLT QVPLRLQILH QGQLLRELQA NVSAQEPGFA 

       370        380        390        400        410        420 
EVLPNLTVQE MDWLVLGELQ MALEWAGRPG LRISGHIAAR KSCDVLQSVL CGADALIPVQ 

       430        440        450        460        470        480 
TGAAGSASLT LLGNGSLIYQ VQVVGTSSEV VAMTLETKPQ RRDQRTVLCH MAGLQPGGHT 

       490        500        510        520        530        540 
AVGICPGLGA RGAHMLLQNE LFLNVGTKDF PDGELRGHVA ALPYCGHSAR HDTLPVPLAG 

       550        560        570        580        590        600 
ALVLPPVKSQ AAGHAWLSLD THCHLHYEVL LAGLGGSEQG TVTAHLLGPP GTPGPRRLLK 

       610        620        630        640        650        660 
GFYGSEAQGV VKDLEPELLR HLAKGMASLM ITTKGSPRGE LRGQVHIANQ CEVGGLRLEA 

       670        680        690        700        710        720 
AGAEGVRALG APDTASAAPP VVPGLPALAP AKPGGPGRPR DPNTCFFEGQ QRPHGARWAP 

       730        740        750        760        770        780 
NYDPLCSLCT CQRRTVICDP VVCPPPSCPH PVQAPDQCCP VCPEKQDVRD LPGLPRSRDP 

       790        800        810        820        830        840 
GEGCYFDGDR SWRAAGTRWH PVVPPFGLIK CAVCTCKGGT GEVHCEKVQC PRLACAQPVR 

       850        860        870        880        890        900 
VNPTDCCKQC PVGSGAHPQL GDPMQADGPR GCRFAGQWFP ESQSWHPSVP PFGEMSCITC 

       910        920        930        940        950 
RCGAGVPHCE RDDCSLPLSC GSGKESRCCS RCTAHRRPAP ETRTDPELEK EAEGS 

« Hide

Isoform 2 [UniParc].

Checksum: 0679D7166DF9F994
Show »

FASTA868,897
Isoform 3 [UniParc].

Checksum: 74479F0AE711A2C4
Show »

FASTA949,913
Isoform 4 [UniParc].

Checksum: F18D4B0ED3EB6A98
Show »

FASTA35037,782
Isoform 5 [UniParc].

Checksum: 506ADC62F09ED062
Show »

FASTA91597,731

References

« Hide 'large scale' references
[1]"The human chordin gene encodes several differentially expressed spliced variants with distinct BMP opposing activities."
Millet C., Lemaire P., Orsetti B., Guglielmi P., Francois V.
Mech. Dev. 106:85-96(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2; 3 AND 4), VARIANT LEU-630.
[2]"The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment."
Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J., Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P., Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E. expand/collapse author list , Heldens S., Huang A., Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D., Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L., Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C., Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J., Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M.
Genome Res. 13:2265-2270(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[4]"Coding sequence and expression patterns of mouse chordin and mapping of the cognate mouse chrd and human CHRD genes."
Pappano W.N., Scott I.C., Clark T.G., Eddy R.L., Shows T.B., Greenspan D.S.
Genomics 52:236-239(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 115-955 (ISOFORM 5), VARIANT LEU-630.
[5]"BMP-binding modules in chordin: a model for signalling regulation in the extracellular space."
Larrain J., Bachiller D., Lu B., Agius E., Piccolo S., De Robertis E.M.
Development 127:821-830(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 51-125; 705-762; 784-850 AND 872-932.
[6]"An unappreciated role for RNA surveillance."
Hillman R.T., Green R.E., Brenner S.E.
Genome Biol. 5:R8.1-R8.16(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: SPLICE ISOFORM(S) THAT ARE POTENTIAL NMD TARGET(S).
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF209928 mRNA. Translation: AAG35767.1.
AF209929 mRNA. Translation: AAG35768.1.
AF209930 mRNA. Translation: AAG35769.1.
AF283325 mRNA. Translation: AAG35784.1.
AY358926 mRNA. Translation: AAQ89285.1.
BC112345 mRNA. Translation: AAI12346.1.
AF076612 mRNA. Translation: AAC69835.1.
AF136632 Genomic DNA. Translation: AAF70236.1.
AF136633 Genomic DNA. Translation: AAF70237.1.
AF136634 Genomic DNA. Translation: AAF70238.1.
AF136635 Genomic DNA. Translation: AAF70239.1.
CCDSCCDS3266.1. [Q9H2X0-1]
RefSeqNP_003732.2. NM_003741.2. [Q9H2X0-1]
UniGeneHs.166186.

3D structure databases

ProteinModelPortalQ9H2X0.
SMRQ9H2X0. Positions 46-131, 700-766, 780-855, 867-937.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid114198. 8 interactions.
DIPDIP-48857N.
IntActQ9H2X0. 11 interactions.
MINTMINT-2817631.

PTM databases

PhosphoSiteQ9H2X0.

Polymorphism databases

DMDM118572631.

Proteomic databases

PaxDbQ9H2X0.
PRIDEQ9H2X0.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000204604; ENSP00000204604; ENSG00000090539. [Q9H2X0-1]
ENST00000310236; ENSP00000308559; ENSG00000090539. [Q9H2X0-3]
ENST00000348986; ENSP00000334036; ENSG00000090539. [Q9H2X0-5]
ENST00000356534; ENSP00000348930; ENSG00000090539. [Q9H2X0-2]
ENST00000420973; ENSP00000392794; ENSG00000090539. [Q9H2X0-4]
ENST00000448472; ENSP00000408624; ENSG00000090539. [Q9H2X0-3]
GeneID8646.
KEGGhsa:8646.
UCSCuc003fov.3. human. [Q9H2X0-1]
uc010hyc.3. human. [Q9H2X0-5]

Organism-specific databases

CTD8646.
GeneCardsGC03P184097.
H-InvDBHIX0003911.
HGNCHGNC:1949. CHRD.
HPACAB020705.
HPA035827.
MIM603475. gene.
neXtProtNX_Q9H2X0.
PharmGKBPA26482.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG85639.
HOVERGENHBG005330.
InParanoidQ9H2X0.
KOK04657.
OMARDPGEGC.
OrthoDBEOG7QNVK7.
PhylomeDBQ9H2X0.
TreeFamTF106451.

Gene expression databases

ArrayExpressQ9H2X0.
BgeeQ9H2X0.
GenevestigatorQ9H2X0.

Family and domain databases

InterProIPR016353. Chordin.
IPR010895. CHRD.
IPR001007. VWF_C.
[Graphical view]
PfamPF07452. CHRD. 4 hits.
PF00093. VWC. 3 hits.
[Graphical view]
PIRSFPIRSF002496. Chordin. 1 hit.
SMARTSM00754. CHRD. 4 hits.
SM00214. VWC. 4 hits.
[Graphical view]
PROSITEPS50933. CHRD. 4 hits.
PS01208. VWFC_1. 2 hits.
PS50184. VWFC_2. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

GenomeRNAi8646.
NextBio32415.
PMAP-CutDBQ9H2X0.
PROQ9H2X0.
SOURCESearch...

Entry information

Entry nameCHRD_HUMAN
AccessionPrimary (citable) accession number: Q9H2X0
Secondary accession number(s): O95254 expand/collapse secondary AC list , Q2M1I8, Q6UW83, Q9H2D3, Q9H2W8, Q9H2W9, Q9P0Z2, Q9P0Z3, Q9P0Z4, Q9P0Z5
Entry history
Integrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: November 28, 2006
Last modified: July 9, 2014
This is version 111 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 3

Human chromosome 3: entries, gene names and cross-references to MIM