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Protein

Zinc finger protein Eos

Gene

IKZF4

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

DNA-binding protein that binds to the 5'GGGAATRCC-3' Ikaros-binding sequence. Transcriptional repressor. Interacts with SPI1 and MITF to repress transcription of the CTSK and ACP5 promoters via recruitment of corepressors SIN3A and CTBP2. May be involved in the development of central and peripheral nervous systems. Essential for the inhibitory function of regulatory T-cells (Treg). Mediates FOXP3-mediated gene silencing in regulatory T-cells (Treg) via recruitment of corepressor CTBP1 (By similarity).By similarity3 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri159 – 18123C2H2-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri187 – 20923C2H2-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri215 – 23723C2H2-type 3PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri248 – 27124C2H2-type 4PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri530 – 55223C2H2-type 5PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri558 – 58225C2H2-type 6PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Zinc finger protein Eos
Alternative name(s):
Ikaros family zinc finger protein 4
Gene namesi
Name:IKZF4
Synonyms:KIAA1782, ZNFN1A4
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 12

Organism-specific databases

HGNCiHGNC:13179. IKZF4.

Subcellular locationi

  • Nucleus 1 Publication

GO - Cellular componenti

  • centrosome Source: HPA
  • nucleoplasm Source: HPA
  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi425 – 4273PED → AAA: No effect on CTBP2 interaction. 1 Publication

Organism-specific databases

PharmGKBiPA162391948.

Polymorphism and mutation databases

BioMutaiIKZF4.
DMDMi158564025.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 585585Zinc finger protein EosPRO_0000299468Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei105 – 1051PhosphoserineBy similarity
Modified residuei335 – 3351N6-acetyllysineBy similarity
Cross-linki500 – 500Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiQ9H2S9.
PaxDbiQ9H2S9.
PRIDEiQ9H2S9.

PTM databases

iPTMnetiQ9H2S9.
PhosphoSiteiQ9H2S9.

Expressioni

Tissue specificityi

Highly expressed in skeletal muscle, low levels of expression in heart, thymus, kidney, liver, and spleen. Expressed in the hematopoietic cell lines MOLT-4, NALM-6 and K-562. Highly expressed in THP-1 and M-07e cell lines, which have characteristics of myeloid and early megakaryocytic cells respectively.1 Publication

Gene expression databases

BgeeiQ9H2S9.
CleanExiHS_IKZF4.
ExpressionAtlasiQ9H2S9. baseline and differential.
GenevisibleiQ9H2S9. HS.

Organism-specific databases

HPAiHPA049016.

Interactioni

Subunit structurei

Self-associates. Interacts with other family members; IKZF1, IKZF2, IKZF3 and IKZF5. Interacts with CTBP2. Interacts with SPI1, MITF, FOXP3 and CTBP1 (By similarity).By similarity1 Publication

Protein-protein interaction databases

BioGridi122146. 16 interactions.
IntActiQ9H2S9. 2 interactions.
STRINGi9606.ENSP00000262032.

Structurei

Secondary structure

1
585
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi173 – 1819Combined sources
Beta strandi187 – 1893Combined sources
Turni190 – 1934Combined sources
Beta strandi194 – 1974Combined sources
Helixi199 – 2057Combined sources
Turni206 – 2094Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2MA7NMR-A155-216[»]
DisProtiDP00376.
ProteinModelPortaliQ9H2S9.
SMRiQ9H2S9. Positions 154-267, 521-561.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni281 – 585305Interaction with FOXP3By similarityAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi425 – 4295CTBP-binding motif PEDLA

Domaini

The N-terminal zinc fingers are involved in sequence-specific DNA binding and heterotypic associations with other family members.
C-terminal zinc fingers mediate homodimerization.

Sequence similaritiesi

Contains 6 C2H2-type zinc fingers.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri159 – 18123C2H2-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri187 – 20923C2H2-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri215 – 23723C2H2-type 3PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri248 – 27124C2H2-type 4PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri530 – 55223C2H2-type 5PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri558 – 58225C2H2-type 6PROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG1721. Eukaryota.
COG5048. LUCA.
GeneTreeiENSGT00550000074392.
HOGENOMiHOG000049114.
HOVERGENiHBG004752.
InParanoidiQ9H2S9.
KOiK09220.
OMAiQPSSDKM.
OrthoDBiEOG79GT61.
PhylomeDBiQ9H2S9.
TreeFamiTF331189.

Family and domain databases

Gene3Di3.30.160.60. 4 hits.
InterProiIPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
SMARTiSM00355. ZnF_C2H2. 6 hits.
[Graphical view]
PROSITEiPS00028. ZINC_FINGER_C2H2_1. 5 hits.
PS50157. ZINC_FINGER_C2H2_2. 4 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9H2S9-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MHTPPALPRR FQGGGRVRTP GSHRQGKDNL ERDPSGGCVP DFLPQAQDSN
60 70 80 90 100
HFIMESLFCE SSGDSSLEKE FLGAPVGPSV STPNSQHSSP SRSLSANSIK
110 120 130 140 150
VEMYSDEESS RLLGPDERLL EKDDSVIVED SLSEPLGYCD GSGPEPHSPG
160 170 180 190 200
GIRLPNGKLK CDVCGMVCIG PNVLMVHKRS HTGERPFHCN QCGASFTQKG
210 220 230 240 250
NLLRHIKLHS GEKPFKCPFC NYACRRRDAL TGHLRTHSVS SPTVGKPYKC
260 270 280 290 300
NYCGRSYKQQ STLEEHKERC HNYLQSLSTE AQALAGQPGD EIRDLEMVPD
310 320 330 340 350
SMLHSSSERP TFIDRLANSL TKRKRSTPQK FVGEKQMRFS LSDLPYDVNS
360 370 380 390 400
GGYEKDVELV AHHSLEPGFG SSLAFVGAEH LRPLRLPPTN CISELTPVIS
410 420 430 440 450
SVYTQMQPLP GRLELPGSRE AGEGPEDLAD GGPLLYRPRG PLTDPGASPS
460 470 480 490 500
NGCQDSTDTE SNHEDRVAGV VSLPQGPPPQ PPPTIVVGRH SPAYAKEDPK
510 520 530 540 550
PQEGLLRGTP GPSKEVLRVV GESGEPVKAF KCEHCRILFL DHVMFTIHMG
560 570 580
CHGFRDPFEC NICGYHSQDR YEFSSHIVRG EHKVG
Length:585
Mass (Da):64,106
Last modified:September 11, 2007 - v2
Checksum:i42AE169A3E2E14D3
GO
Isoform 2 (identifier: Q9H2S9-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-61: MHTPPALPRR...FIMESLFCES → MDSRYLQLQLYLPSCSLLQG

Note: No experimental confirmation available.
Show »
Length:544
Mass (Da):59,671
Checksum:i68743F2C2449BD84
GO

Sequence cautioni

The sequence AAG39221.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence BAB47411.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti145 – 1451E → K in AAG39221 (PubMed:10978333).Curated
Sequence conflicti310 – 3101P → A in AAG39221 (PubMed:10978333).Curated
Sequence conflicti361 – 3611A → G in AAG39221 (PubMed:10978333).Curated
Sequence conflicti390 – 3901N → H in AAG39221 (PubMed:10978333).Curated
Sequence conflicti524 – 5241G → S in AAG39221 (PubMed:10978333).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 6161MHTPP…LFCES → MDSRYLQLQLYLPSCSLLQG in isoform 2. 1 PublicationVSP_027688Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB058685 mRNA. Translation: BAB47411.1. Different initiation.
BX647761 mRNA. No translation available.
AF230809 mRNA. Translation: AAG39221.1. Different initiation.
CCDSiCCDS44917.1. [Q9H2S9-1]
RefSeqiNP_071910.3. NM_022465.3. [Q9H2S9-1]
XP_011536969.1. XM_011538667.1. [Q9H2S9-2]
UniGeneiHs.553221.

Genome annotation databases

EnsembliENST00000262032; ENSP00000262032; ENSG00000123411. [Q9H2S9-1]
ENST00000431367; ENSP00000412101; ENSG00000123411. [Q9H2S9-1]
ENST00000547167; ENSP00000448419; ENSG00000123411. [Q9H2S9-1]
GeneIDi64375.
KEGGihsa:64375.
UCSCiuc001sjb.1. human. [Q9H2S9-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB058685 mRNA. Translation: BAB47411.1. Different initiation.
BX647761 mRNA. No translation available.
AF230809 mRNA. Translation: AAG39221.1. Different initiation.
CCDSiCCDS44917.1. [Q9H2S9-1]
RefSeqiNP_071910.3. NM_022465.3. [Q9H2S9-1]
XP_011536969.1. XM_011538667.1. [Q9H2S9-2]
UniGeneiHs.553221.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2MA7NMR-A155-216[»]
DisProtiDP00376.
ProteinModelPortaliQ9H2S9.
SMRiQ9H2S9. Positions 154-267, 521-561.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi122146. 16 interactions.
IntActiQ9H2S9. 2 interactions.
STRINGi9606.ENSP00000262032.

PTM databases

iPTMnetiQ9H2S9.
PhosphoSiteiQ9H2S9.

Polymorphism and mutation databases

BioMutaiIKZF4.
DMDMi158564025.

Proteomic databases

MaxQBiQ9H2S9.
PaxDbiQ9H2S9.
PRIDEiQ9H2S9.

Protocols and materials databases

DNASUi64375.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000262032; ENSP00000262032; ENSG00000123411. [Q9H2S9-1]
ENST00000431367; ENSP00000412101; ENSG00000123411. [Q9H2S9-1]
ENST00000547167; ENSP00000448419; ENSG00000123411. [Q9H2S9-1]
GeneIDi64375.
KEGGihsa:64375.
UCSCiuc001sjb.1. human. [Q9H2S9-1]

Organism-specific databases

CTDi64375.
GeneCardsiIKZF4.
HGNCiHGNC:13179. IKZF4.
HPAiHPA049016.
MIMi606239. gene.
neXtProtiNX_Q9H2S9.
PharmGKBiPA162391948.
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1721. Eukaryota.
COG5048. LUCA.
GeneTreeiENSGT00550000074392.
HOGENOMiHOG000049114.
HOVERGENiHBG004752.
InParanoidiQ9H2S9.
KOiK09220.
OMAiQPSSDKM.
OrthoDBiEOG79GT61.
PhylomeDBiQ9H2S9.
TreeFamiTF331189.

Miscellaneous databases

GeneWikiiIKZF4.
GenomeRNAii64375.
PROiQ9H2S9.
SOURCEiSearch...

Gene expression databases

BgeeiQ9H2S9.
CleanExiHS_IKZF4.
ExpressionAtlasiQ9H2S9. baseline and differential.
GenevisibleiQ9H2S9. HS.

Family and domain databases

Gene3Di3.30.160.60. 4 hits.
InterProiIPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
SMARTiSM00355. ZnF_C2H2. 6 hits.
[Graphical view]
PROSITEiPS00028. ZINC_FINGER_C2H2_1. 5 hits.
PS50157. ZINC_FINGER_C2H2_2. 4 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Prediction of the coding sequences of unidentified human genes. XX. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro."
    Nagase T., Nakayama M., Nakajima D., Kikuno R., Ohara O.
    DNA Res. 8:85-95(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Brain.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
  3. "Eos and pegasus, two members of the Ikaros family of proteins with distinct DNA binding activities."
    Perdomo J., Holmes M., Chong B., Crossley M.
    J. Biol. Chem. 275:38347-38354(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 53-585 (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY, INTERACTION WITH IKZF1; IKZF2; IKZF3 AND IKZF5.
  4. "A molecular dissection of the repression circuitry of Ikaros."
    Koipally J., Georgopoulos K.
    J. Biol. Chem. 277:27697-27705(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  5. "The Ikaros family protein Eos associates with C-terminal-binding protein corepressors."
    Perdomo J., Crossley M.
    Eur. J. Biochem. 269:5885-5892(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH CTBP2, SUBCELLULAR LOCATION, MUTAGENESIS OF 425-PRO--ASP-427.
  6. "Solution NMR structure of zinc finger protein EOS from Homo sapiens, Northeast structural genomics consortium (NESG) target HR7992A."
    Northeast structural genomics consortium (NESG)
    Submitted (SEP-2013) to the PDB data bank
    Cited for: STRUCTURE BY NMR OF 155-216 IN COMPLEX WITH ZINC IONS.

Entry informationi

Entry nameiIKZF4_HUMAN
AccessioniPrimary (citable) accession number: Q9H2S9
Secondary accession number(s): Q96JP3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: September 11, 2007
Last modified: June 8, 2016
This is version 120 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Miscellaneous

'Eos' means 'rising sun' in Greek.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 12
    Human chromosome 12: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.