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Reviewed, UniProtKB/Swiss-Prot Q9H2S9 (IKZF4_HUMAN)

Last modified January 19, 2010. Version 56. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Zinc finger protein Eos
Alternative name(s):
    Ikaros family zinc finger protein 4
Gene names
Name: IKZF4
Synonyms: KIAA1782, ZNFN1A4
OrganismHomo sapiens (Human) [Complete proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length585 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

DNA-binding protein that binds to the 5'GGGAATRCC-3' Ikaros-binding sequence. Transcriptional repressor. Interacts with SPI1 and MITF to repress transcription of the CTSK and ACP5 promoters via recruitment of corepressors SIN3A and CTBP2. May be involved in the development of central and peripheral nervous systems. Ref.3 Ref.4 Ref.5

Subunit structure

Self-associates and interacts with other family members; IKZF1, IKZF2, IKZF3 AND IKZF5. Interacts with CTBP2, SPI1 and MITF By similarity. Ref.3 Ref.5

Subcellular location

Nucleus Ref.5.

Tissue specificity

Highly expressed in skeletal muscle, low levels of expression in heart, thymus, kidney, liver, and spleen. Expressed in the hematopoietic cell lines MOLT-4, NALM-6 and K562. Highly expressed in THP-1 and Mo7e-IL3 cell lines, which have characteristics of myeloid and early megakaryocytic cells respectively. Ref.3

Domain

The N-terminal zinc fingers are involved in sequence-specific DNA binding and heterotypic associations with other family members.

C-terminal zinc fingers mediate homodimerization.

Miscellaneous

'Eos' is a Greek word for rising sun.

Sequence similarities

Belongs to the Ikaros C2H2-type zinc-finger protein family.

Contains 6 C2H2-type zinc fingers.

Ontologies

Keywords
   Biological processTranscription
Transcription regulation
   Cellular componentNucleus
   Coding sequence diversityAlternative splicing
   DomainRepeat
Zinc-finger
   LigandDNA-binding
Metal-binding
Zinc
   Molecular functionRepressor
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processregulation of transcription

Inferred from electronic annotation. Source: UniProtKB-KW

transcription

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentnucleus Ref.3

Traceable author statement. Source: UniProtKB

   Molecular functiontranscription factor activity Ref.3

Traceable author statement. Source: UniProtKB

transcription repressor activity Ref.3

Traceable author statement. Source: UniProtKB

zinc ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9H2S9-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9H2S9-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-61: MHTPPALPRR...FIMESLFCES → MDSRYLQLQLYLPSCSLLQG
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 585585Zinc finger protein Eos
PRO_0000299468

Regions

Zinc finger159 – 18123C2H2-type 1
Zinc finger187 – 20923C2H2-type 2
Zinc finger215 – 23723C2H2-type 3
Zinc finger248 – 27124C2H2-type 4
Zinc finger530 – 55223C2H2-type 5
Zinc finger558 – 58225C2H2-type 6
Motif425 – 4295CTBP-binding motif PEDLA

Natural variations

Alternative sequence1 – 6161MHTPP…LFCES → MDSRYLQLQLYLPSCSLLQG in isoform 2.
VSP_027688

Experimental info

Mutagenesis425 – 4273PED → AAA: No effect on CTBP2 interaction. Ref.5
Sequence conflict1451E → K in AAG39221. Ref.3
Sequence conflict3101P → A in AAG39221. Ref.3
Sequence conflict3611A → G in AAG39221. Ref.3
Sequence conflict3901N → H in AAG39221. Ref.3
Sequence conflict5241G → S in AAG39221. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified September 11, 2007. Version 2.
Checksum: 42AE169A3E2E14D3

FASTA58564,106
        10         20         30         40         50         60 
MHTPPALPRR FQGGGRVRTP GSHRQGKDNL ERDPSGGCVP DFLPQAQDSN HFIMESLFCE 

        70         80         90        100        110        120 
SSGDSSLEKE FLGAPVGPSV STPNSQHSSP SRSLSANSIK VEMYSDEESS RLLGPDERLL 

       130        140        150        160        170        180 
EKDDSVIVED SLSEPLGYCD GSGPEPHSPG GIRLPNGKLK CDVCGMVCIG PNVLMVHKRS 

       190        200        210        220        230        240 
HTGERPFHCN QCGASFTQKG NLLRHIKLHS GEKPFKCPFC NYACRRRDAL TGHLRTHSVS 

       250        260        270        280        290        300 
SPTVGKPYKC NYCGRSYKQQ STLEEHKERC HNYLQSLSTE AQALAGQPGD EIRDLEMVPD 

       310        320        330        340        350        360 
SMLHSSSERP TFIDRLANSL TKRKRSTPQK FVGEKQMRFS LSDLPYDVNS GGYEKDVELV 

       370        380        390        400        410        420 
AHHSLEPGFG SSLAFVGAEH LRPLRLPPTN CISELTPVIS SVYTQMQPLP GRLELPGSRE 

       430        440        450        460        470        480 
AGEGPEDLAD GGPLLYRPRG PLTDPGASPS NGCQDSTDTE SNHEDRVAGV VSLPQGPPPQ 

       490        500        510        520        530        540 
PPPTIVVGRH SPAYAKEDPK PQEGLLRGTP GPSKEVLRVV GESGEPVKAF KCEHCRILFL 

       550        560        570        580 
DHVMFTIHMG CHGFRDPFEC NICGYHSQDR YEFSSHIVRG EHKVG 

« Hide

Isoform 2.

Checksum: 68743F2C2449BD84
Show »

FASTA54459,671

References

« Hide 'large scale' references
[1]"Prediction of the coding sequences of unidentified human genes. XX. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro."
Nagase T., Nakayama M., Nakajima D., Kikuno R., Ohara O.
DNA Res. 8:85-95(2001) [PubMed: 11347906] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Tissue: Brain.
[2]"The full-ORF clone resource of the German cDNA consortium."
Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I.
BMC Genomics 8:399-399(2007) [PubMed: 17974005] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[3]"Eos and pegasus, two members of the Ikaros family of proteins with distinct DNA binding activities."
Perdomo J., Holmes M., Chong B., Crossley M.
J. Biol. Chem. 275:38347-38354(2000) [PubMed: 10978333] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 53-585 (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY, INTERACTION WITH IKZF1; IKZF2; IKZF3 AND IKZF5.
[4]"A molecular dissection of the repression circuitry of Ikaros."
Koipally J., Georgopoulos K.
J. Biol. Chem. 277:27697-27705(2002) [PubMed: 12015313] [Abstract]
Cited for: FUNCTION.
[5]"The Ikaros family protein Eos associates with C-terminal-binding protein corepressors."
Perdomo J., Crossley M.
Eur. J. Biochem. 269:5885-5892(2002) [PubMed: 12444977] [Abstract]
Cited for: FUNCTION, INTERACTION WITH CTBP2, SUBCELLULAR LOCATION, MUTAGENESIS OF 425-PRO--ASP-427.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB058685 mRNA. Translation: BAB47411.1. Different initiation.
BX647761 mRNA. No translation available.
AF230809 mRNA. Translation: AAG39221.1. Different initiation.
IPIIPI00064241.
IPI00797730.
RefSeqNP_071910.3.
UniGeneHs.553221

3D structure databases

HSSPHSSP built from PDB template 2CT1 based on UniProtKB P49711.
SMRQ9H2S9. Positions 159-263.
ModBaseSearch...

Protein-protein interaction databases

IntActQ9H2S9. 2 interactions.
STRINGQ9H2S9.

Proteomic databases

PRIDEQ9H2S9.

Genome annotation databases

EnsemblENST00000262032; ENSP00000262032; ENSG00000123411; Homo sapiens. [Genome view]
GeneID64375.
KEGGhsa:64375.
UCSCuc001sjb.1. human.
uc001sje.1. human.

Organism-specific databases

CTD64375.
GeneCardsGC12P054705.
H-InvDBHIX0010708.
HGNCHGNC:13179. IKZF4.
MIM606239. gene.
HUGESearch...
GenAtlasSearch...

Phylogenomic databases

eggNOGprNOG19881.
HOGENOMHBG447329.
HOVERGENQ9H2S9.
InParanoidQ9H2S9.
OMAYAKEDPK.
OrthoDBEOG9PG8K4.

Gene expression databases

ArrayExpressQ9H2S9.
BgeeQ9H2S9.
CleanExHS_IKZF4.
GenevestigatorQ9H2S9.

Family and domain databases

InterProIPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
Gene3DG3DSA:3.30.160.60. Znf_C2H2/integrase_DNA-bd. 1 hit.
PfamPF00096. zf-C2H2. 1 hit.
[Graphical view]
SMARTSM00355. ZnF_C2H2. 6 hits.
[Graphical view]
PROSITEPS00028. ZINC_FINGER_C2H2_1. 5 hits.
PS50157. ZINC_FINGER_C2H2_2. 4 hits.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio66301.
SOURCESearch...

Entry information

Entry nameIKZF4_HUMAN
AccessionPrimary (citable) accession number: Q9H2S9
Secondary accession number(s): Q96JP3
Entry history
Integrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: September 11, 2007
Last modified: January 19, 2010
This is version 56 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

Human chromosome 12

Human chromosome 12: entries, gene names and cross-references to MIM

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents