ID   KLK15_HUMAN             Reviewed;         256 AA.
AC   Q9H2R5; A0AUY8; Q15358; Q6ISI0; Q9H2R3; Q9H2R4; Q9H2R6; Q9HBG9;
DT   18-OCT-2001, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   27-MAR-2024, entry version 176.
DE   RecName: Full=Kallikrein-15;
DE            EC=3.4.21.-;
DE   AltName: Full=ACO protease;
DE   Flags: Precursor;
GN   Name=KLK15;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], ALTERNATIVE SPLICING, AND TISSUE
RP   SPECIFICITY.
RX   PubMed=11010966; DOI=10.1074/jbc.m005432200;
RA   Yousef G.M., Scorilas A., Jung K., Ashworth L.K., Diamandis E.P.;
RT   "Molecular cloning of the human kallikrein 15 gene (KLK15). Up-regulation
RT   in prostate cancer.";
RL   J. Biol. Chem. 276:53-61(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=11054574; DOI=10.1016/s0378-1119(00)00382-6;
RA   Gan L., Lee I., Smith R., Argonza-Barrett R., Lei H., McCuaig J., Moss P.,
RA   Paeper B., Wang K.;
RT   "Sequencing and expression analysis of the serine protease gene cluster
RT   located in chromosome 19q13 region.";
RL   Gene 257:119-130(2000).
RN   [3]
RP   PRELIMINARY PARTIAL NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Brain;
RX   PubMed=8018728; DOI=10.1016/0167-4781(94)90018-3;
RA   Dihanich M.E., Spiess M.;
RT   "A novel serine proteinase-like sequence from human brain.";
RL   Biochim. Biophys. Acta 1218:225-228(1994).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Mural R.J., Istrail S., Sutton G., Florea L., Halpern A.L., Mobarry C.M.,
RA   Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA   Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA   Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA   Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA   Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA   Hunkapiller M.W., Myers E.W., Venter J.C.;
RL   Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 5).
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [6]
RP   SPLICE ISOFORM(S) THAT ARE POTENTIAL NMD TARGET(S).
RX   PubMed=14759258; DOI=10.1186/gb-2004-5-2-r8;
RA   Hillman R.T., Green R.E., Brenner S.E.;
RT   "An unappreciated role for RNA surveillance.";
RL   Genome Biol. 5:R8.1-R8.16(2004).
RN   [7]
RP   VARIANT [LARGE SCALE ANALYSIS] THR-137.
RX   PubMed=16959974; DOI=10.1126/science.1133427;
RA   Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D.,
RA   Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P.,
RA   Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V.,
RA   Gazdar A.F., Hartigan J., Wu L., Liu C., Parmigiani G., Park B.H.,
RA   Bachman K.E., Papadopoulos N., Vogelstein B., Kinzler K.W.,
RA   Velculescu V.E.;
RT   "The consensus coding sequences of human breast and colorectal cancers.";
RL   Science 314:268-274(2006).
CC   -!- FUNCTION: Protease whose physiological substrate is not yet known.
CC   -!- INTERACTION:
CC       Q9H2R5; P63172: DYNLT1; NbExp=3; IntAct=EBI-8645371, EBI-1176455;
CC       Q9H2R5; Q15654: TRIP6; NbExp=3; IntAct=EBI-8645371, EBI-742327;
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=5;
CC       Name=1;
CC         IsoId=Q9H2R5-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=Q9H2R5-2; Sequence=VSP_005405;
CC       Name=3;
CC         IsoId=Q9H2R5-3; Sequence=VSP_005406, VSP_005407;
CC       Name=4;
CC         IsoId=Q9H2R5-4; Sequence=VSP_005404;
CC       Name=5;
CC         IsoId=Q9H2R5-5; Sequence=VSP_054621;
CC   -!- TISSUE SPECIFICITY: Highest expression in the thyroid gland. Also
CC       expressed in the prostate, salivary, and adrenal glands and in the
CC       colon testis and kidney. {ECO:0000269|PubMed:11010966}.
CC   -!- MISCELLANEOUS: [Isoform 2]: May be produced at very low levels due to a
CC       premature stop codon in the mRNA, leading to nonsense-mediated mRNA
CC       decay. {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the peptidase S1 family. Kallikrein subfamily.
CC       {ECO:0000255|PROSITE-ProRule:PRU00274}.
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DR   EMBL; AF242195; AAG09469.1; -; Genomic_DNA.
DR   EMBL; AF242195; AAG09470.1; -; Genomic_DNA.
DR   EMBL; AF242195; AAG09471.1; -; Genomic_DNA.
DR   EMBL; AF242195; AAG09472.1; -; Genomic_DNA.
DR   EMBL; AF243527; AAG33354.1; -; Genomic_DNA.
DR   EMBL; X75363; CAA53145.1; ALT_SEQ; mRNA.
DR   EMBL; CH471135; EAW71918.1; -; Genomic_DNA.
DR   EMBL; BC069480; AAH69480.1; -; mRNA.
DR   EMBL; BC069507; AAH69507.1; -; mRNA.
DR   EMBL; BC069518; AAH69518.1; -; mRNA.
DR   EMBL; BC126137; AAI26138.1; -; mRNA.
DR   EMBL; BC144046; AAI44047.1; -; mRNA.
DR   CCDS; CCDS12805.1; -. [Q9H2R5-1]
DR   CCDS; CCDS62766.1; -. [Q9H2R5-5]
DR   PIR; S45356; S45356.
DR   RefSeq; NP_001264010.1; NM_001277081.1. [Q9H2R5-5]
DR   RefSeq; NP_001264011.1; NM_001277082.1.
DR   RefSeq; NP_059979.2; NM_017509.3. [Q9H2R5-1]
DR   RefSeq; XP_006723328.1; XM_006723265.3.
DR   RefSeq; XP_011525387.1; XM_011527085.2.
DR   RefSeq; XP_011525389.1; XM_011527087.2. [Q9H2R5-4]
DR   RefSeq; XP_011525390.1; XM_011527088.2. [Q9H2R5-3]
DR   RefSeq; XP_016882432.1; XM_017026943.1. [Q9H2R5-2]
DR   AlphaFoldDB; Q9H2R5; -.
DR   SMR; Q9H2R5; -.
DR   BioGRID; 120715; 187.
DR   IntAct; Q9H2R5; 7.
DR   MINT; Q9H2R5; -.
DR   STRING; 9606.ENSP00000469315; -.
DR   MEROPS; S01.081; -.
DR   GlyCosmos; Q9H2R5; 2 sites, No reported glycans.
DR   GlyGen; Q9H2R5; 2 sites.
DR   iPTMnet; Q9H2R5; -.
DR   PhosphoSitePlus; Q9H2R5; -.
DR   BioMuta; KLK15; -.
DR   DMDM; 18202940; -.
DR   jPOST; Q9H2R5; -.
DR   MassIVE; Q9H2R5; -.
DR   PaxDb; 9606-ENSP00000469315; -.
DR   PeptideAtlas; Q9H2R5; -.
DR   ProteomicsDB; 80579; -. [Q9H2R5-1]
DR   Antibodypedia; 18915; 275 antibodies from 28 providers.
DR   DNASU; 55554; -.
DR   Ensembl; ENST00000326856.8; ENSP00000314783.4; ENSG00000174562.14. [Q9H2R5-5]
DR   Ensembl; ENST00000598239.6; ENSP00000469315.1; ENSG00000174562.14. [Q9H2R5-1]
DR   Ensembl; ENST00000695965.1; ENSP00000512291.1; ENSG00000174562.14. [Q9H2R5-4]
DR   Ensembl; ENST00000695998.1; ENSP00000512319.1; ENSG00000174562.14. [Q9H2R5-5]
DR   GeneID; 55554; -.
DR   KEGG; hsa:55554; -.
DR   MANE-Select; ENST00000598239.6; ENSP00000469315.1; NM_017509.4; NP_059979.2.
DR   UCSC; uc002ptl.4; human. [Q9H2R5-1]
DR   AGR; HGNC:20453; -.
DR   CTD; 55554; -.
DR   DisGeNET; 55554; -.
DR   GeneCards; KLK15; -.
DR   HGNC; HGNC:20453; KLK15.
DR   HPA; ENSG00000174562; Tissue enhanced (intestine, salivary gland, testis).
DR   MIM; 610601; gene.
DR   neXtProt; NX_Q9H2R5; -.
DR   OpenTargets; ENSG00000174562; -.
DR   PharmGKB; PA134977502; -.
DR   VEuPathDB; HostDB:ENSG00000174562; -.
DR   eggNOG; KOG3627; Eukaryota.
DR   GeneTree; ENSGT00940000162074; -.
DR   HOGENOM; CLU_006842_1_1_1; -.
DR   InParanoid; Q9H2R5; -.
DR   OMA; IISDASC; -.
DR   OrthoDB; 4629979at2759; -.
DR   PhylomeDB; Q9H2R5; -.
DR   TreeFam; TF331065; -.
DR   BRENDA; 3.4.21.35; 2681.
DR   PathwayCommons; Q9H2R5; -.
DR   SignaLink; Q9H2R5; -.
DR   BioGRID-ORCS; 55554; 14 hits in 1149 CRISPR screens.
DR   ChiTaRS; KLK15; human.
DR   GeneWiki; KLK15; -.
DR   GenomeRNAi; 55554; -.
DR   Pharos; Q9H2R5; Tbio.
DR   PRO; PR:Q9H2R5; -.
DR   Proteomes; UP000005640; Chromosome 19.
DR   RNAct; Q9H2R5; Protein.
DR   Bgee; ENSG00000174562; Expressed in vena cava and 77 other cell types or tissues.
DR   ExpressionAtlas; Q9H2R5; baseline and differential.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0030141; C:secretory granule; IBA:GO_Central.
DR   GO; GO:0004252; F:serine-type endopeptidase activity; NAS:UniProtKB.
DR   GO; GO:0008236; F:serine-type peptidase activity; TAS:ProtInc.
DR   GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR   CDD; cd00190; Tryp_SPc; 1.
DR   Gene3D; 2.40.10.10; Trypsin-like serine proteases; 2.
DR   InterPro; IPR009003; Peptidase_S1_PA.
DR   InterPro; IPR043504; Peptidase_S1_PA_chymotrypsin.
DR   InterPro; IPR001314; Peptidase_S1A.
DR   InterPro; IPR001254; Trypsin_dom.
DR   InterPro; IPR018114; TRYPSIN_HIS.
DR   PANTHER; PTHR24271:SF60; KALLIKREIN-15; 1.
DR   PANTHER; PTHR24271; KALLIKREIN-RELATED; 1.
DR   Pfam; PF00089; Trypsin; 1.
DR   PRINTS; PR00722; CHYMOTRYPSIN.
DR   SMART; SM00020; Tryp_SPc; 1.
DR   SUPFAM; SSF50494; Trypsin-like serine proteases; 1.
DR   PROSITE; PS50240; TRYPSIN_DOM; 1.
DR   PROSITE; PS00134; TRYPSIN_HIS; 1.
DR   Genevisible; Q9H2R5; HS.
PE   1: Evidence at protein level;
KW   Alternative splicing; Disulfide bond; Glycoprotein; Hydrolase; Protease;
KW   Reference proteome; Secreted; Serine protease; Signal; Zymogen.
FT   SIGNAL          1..16
FT                   /evidence="ECO:0000255"
FT   PROPEP          17..21
FT                   /note="Activation peptide"
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_0000027960"
FT   CHAIN           22..256
FT                   /note="Kallikrein-15"
FT                   /id="PRO_0000027961"
FT   DOMAIN          22..254
FT                   /note="Peptidase S1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00274"
FT   ACT_SITE        62
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        106
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        209
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250"
FT   CARBOHYD        171
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        232
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   DISULFID        47..63
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00274"
FT   DISULFID        138..215
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00274"
FT   DISULFID        180..194
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00274"
FT   DISULFID        205..230
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00274"
FT   VAR_SEQ         15
FT                   /note="Missing (in isoform 5)"
FT                   /evidence="ECO:0000303|PubMed:15489334"
FT                   /id="VSP_054621"
FT   VAR_SEQ         122..256
FT                   /note="Missing (in isoform 2)"
FT                   /evidence="ECO:0000305"
FT                   /id="VSP_005405"
FT   VAR_SEQ         122..206
FT                   /note="Missing (in isoform 4)"
FT                   /evidence="ECO:0000305"
FT                   /id="VSP_005404"
FT   VAR_SEQ         161
FT                   /note="V -> G (in isoform 3)"
FT                   /evidence="ECO:0000305"
FT                   /id="VSP_005406"
FT   VAR_SEQ         162..256
FT                   /note="Missing (in isoform 3)"
FT                   /evidence="ECO:0000305"
FT                   /id="VSP_005407"
FT   VARIANT         134
FT                   /note="P -> L (in dbSNP:rs3212805)"
FT                   /id="VAR_020179"
FT   VARIANT         137
FT                   /note="A -> T (in a breast cancer sample; somatic
FT                   mutation)"
FT                   /evidence="ECO:0000269|PubMed:16959974"
FT                   /id="VAR_036298"
FT   CONFLICT        147..160
FT                   /note="SHNEPGTAGSPRSQ -> PLSSP (in Ref. 2)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   256 AA;  28087 MW;  B5EBF8D6022786B5 CRC64;
     MWLLLTLSFL LASTAAQDGD KLLEGDECAP HSQPWQVALY ERGRFNCGAS LISPHWVLSA
     AHCQSRFMRV RLGEHNLRKR DGPEQLRTTS RVIPHPRYEA RSHRNDIMLL RLVQPARLNP
     QVRPAVLPTR CPHPGEACVV SGWGLVSHNE PGTAGSPRSQ VSLPDTLHCA NISIISDTSC
     DKSYPGRLTN TMVCAGAEGR GAESCEGDSG GPLVCGGILQ GIVSWGDVPC DNTTKPGVYT
     KVCHYLEWIR ETMKRN
//