Reviewed, UniProtKB/Swiss-Prot Q9H2R5 (KLK15_HUMAN)

Names and origin

Protein names

Recommended name:
    Kallikrein-15
    EC=3.4.21.-
Alternative name(s):
    ACO protease

Gene names

Name:

KLK15

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	256 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Protease whose physiological substrate is not yet known.
Subcellular location	Secreted Probable.
Tissue specificity	Highest expression in the thyroid gland. Also expressed in the prostate, salivary, and adrenal glands and in the colon testis and kidney. Ref.1
Sequence similarities	Belongs to the peptidase S1 family. Kallikrein subfamily. Contains 1 peptidase S1 domain.

Ontologies

Keywords
Cellular component	Secreted
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Signal
Molecular function	Hydrolase Protease Serine protease
PTM	Disulfide bond Glycoprotein Zymogen
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Non-traceable author statement. Source: UniProtKB
Cellular component	extracellular region Non-traceable author statement. Source: UniProtKB
Molecular function	serine-type endopeptidase activity Ref.1 Non-traceable author statement. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9H2R5-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9H2R5-2)

The sequence of this isoform differs from the canonical sequence as follows:
     122-256: Missing.

Note: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.

Isoform 3 (identifier: Q9H2R5-3)

The sequence of this isoform differs from the canonical sequence as follows:
     161-161: V → G
     162-256: Missing.

Isoform 4 (identifier: Q9H2R5-4)

The sequence of this isoform differs from the canonical sequence as follows:
     122-206: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 16	16	Potential
	Propeptide	17 – 21	5	Activation peptide Potential		PRO_0000027960
	Chain	22 – 256	235	Kallikrein-15		PRO_0000027961
Regions
	Domain	22 – 254	233	Peptidase S1
Sites
	Active site	62	1	Charge relay system By similarity
	Active site	106	1	Charge relay system By similarity
	Active site	209	1	Charge relay system By similarity
Amino acid modifications
	Glycosylation	171	1	N-linked (GlcNAc...) Potential
	Glycosylation	232	1	N-linked (GlcNAc...) Potential
	Disulfide bond	47 ↔ 63		By similarity
	Disulfide bond	138 ↔ 215		By similarity
	Disulfide bond	180 ↔ 194		By similarity
	Disulfide bond	205 ↔ 230		By similarity
Natural variations
	Alternative sequence	122 – 256	135	Missing in isoform 2.		VSP_005405
	Alternative sequence	122 – 206	85	Missing in isoform 4.		VSP_005404
	Alternative sequence	161	1	V → G in isoform 3.		VSP_005406
	Alternative sequence	162 – 256	95	Missing in isoform 3.		VSP_005407
	Natural variant	134	1	P → L: dbSNP rs3212805.		VAR_020179
	Natural variant	137	1	A → T in a breast cancer sample; somatic mutation. Ref.6		VAR_036298
Experimental info
	Sequence conflict	147 – 160	14	SHNEP…SPRSQ → PLSSP Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: B5EBF8D6022786B5	FASTA	256	28,087
	10 20 30 40 50 60 MWLLLTLSFL LASTAAQDGD KLLEGDECAP HSQPWQVALY ERGRFNCGAS LISPHWVLSA 70 80 90 100 110 120 AHCQSRFMRV RLGEHNLRKR DGPEQLRTTS RVIPHPRYEA RSHRNDIMLL RLVQPARLNP 130 140 150 160 170 180 QVRPAVLPTR CPHPGEACVV SGWGLVSHNE PGTAGSPRSQ VSLPDTLHCA NISIISDTSC 190 200 210 220 230 240 DKSYPGRLTN TMVCAGAEGR GAESCEGDSG GPLVCGGILQ GIVSWGDVPC DNTTKPGVYT 250 KVCHYLEWIR ETMKRN
	Isoform 2. Checksum: 43BD650A394BF369	FASTA	121	13,907
	10 20 30 40 50 60 MWLLLTLSFL LASTAAQDGD KLLEGDECAP HSQPWQVALY ERGRFNCGAS LISPHWVLSA 70 80 90 100 110 120 AHCQSRFMRV RLGEHNLRKR DGPEQLRTTS RVIPHPRYEA RSHRNDIMLL RLVQPARLNP Q
	Isoform 3. Checksum: 539D5C6163B16285	FASTA	161	17,954
	10 20 30 40 50 60 MWLLLTLSFL LASTAAQDGD KLLEGDECAP HSQPWQVALY ERGRFNCGAS LISPHWVLSA 70 80 90 100 110 120 AHCQSRFMRV RLGEHNLRKR DGPEQLRTTS RVIPHPRYEA RSHRNDIMLL RLVQPARLNP 130 140 150 160 QVRPAVLPTR CPHPGEACVV SGWGLVSHNE PGTAGSPRSQ G
	Isoform 4. Checksum: 20CDE27B3E7B89E4	FASTA	171	19,341
	10 20 30 40 50 60 MWLLLTLSFL LASTAAQDGD KLLEGDECAP HSQPWQVALY ERGRFNCGAS LISPHWVLSA 70 80 90 100 110 120 AHCQSRFMRV RLGEHNLRKR DGPEQLRTTS RVIPHPRYEA RSHRNDIMLL RLVQPARLNP 130 140 150 160 170 QGDSGGPLVC GGILQGIVSW GDVPCDNTTK PGVYTKVCHY LEWIRETMKR N

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning of the human kallikrein 15 gene (KLK15). Up-regulation in prostate cancer." Yousef G.M., Scorilas A., Jung K., Ashworth L.K., Diamandis E.P. J. Biol. Chem. 276:53-61(2001) [PubMed: 11010966] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], ALTERNATIVE SPLICING, TISSUE SPECIFICITY.
[2]	"Sequencing and expression analysis of the serine protease gene cluster located in chromosome 19q13 region." Gan L., Lee I., Smith R., Argonza-Barrett R., Lei H., McCuaig J., Moss P., Paeper B., Wang K. Gene 257:119-130(2000) [PubMed: 11054574] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"A novel serine proteinase-like sequence from human brain." Dihanich M.E., Spiess M. Biochim. Biophys. Acta 1218:225-228(1994) [PubMed: 8018728] [Abstract] Cited for: PRELIMINARY PARTIAL NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[5]	"An unappreciated role for RNA surveillance." Hillman R.T., Green R.E., Brenner S.E. Genome Biol. 5:RESEARCH008.1-RESEARCH008.16(2004) [PubMed: 14759258] [Abstract] Cited for: SPLICE ISOFORM(S) THAT ARE POTENTIAL NMD TARGET(S).
[6]	"The consensus coding sequences of human breast and colorectal cancers." Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D., Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P., Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V. , Gazdar A.F., Hartigan J., Wu L., Liu C., Parmigiani G., Park B.H., Bachman K.E., Papadopoulos N., Vogelstein B., Kinzler K.W., Velculescu V.E. Science 314:268-274(2006) [PubMed: 16959974] [Abstract] Cited for: VARIANT [LARGE SCALE ANALYSIS] THR-137.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF242195 Genomic DNA. Translation: AAG09469.1. AF242195 Genomic DNA. Translation: AAG09470.1. AF242195 Genomic DNA. Translation: AAG09471.1. AF242195 Genomic DNA. Translation: AAG09472.1. AF243527 Genomic DNA. Translation: AAG33354.1. X75363 mRNA. Translation: CAA53145.1. Sequence problems. BC069507 mRNA. Translation: AAH69507.1. BC126137 mRNA. Translation: AAI26138.1.
IPI	IPI00033970. IPI00150552. IPI00219692. IPI00219693.
PIR	S45356.
RefSeq	NP_059979.2. NP_612630.1. NP_612631.1.
UniGene	Hs.567535
3D structure databases
HSSP	HSSP built from PDB template 1EZX based on UniProtKB P00760.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9H2R5.
Protein family/group databases
MEROPS	S01.081.
Proteomic databases
PRIDE	Q9H2R5.
Genome annotation databases
Ensembl	ENST00000301421; ENSP00000301421; ENSG00000174562; Homo sapiens. [Genome view] ENST00000326856; ENSP00000314783; ENSG00000174562; Homo sapiens. [Genome view] ENST00000416184; ENSP00000415136; ENSG00000174562; Homo sapiens. [Genome view]
GeneID	55554.
KEGG	hsa:55554.
UCSC	uc002ptl.1. human. uc002ptm.1. human. uc002ptn.1. human.
Organism-specific databases
CTD	55554.
GeneCards	GC19M056020.
H-InvDB	HIX0039991.
HGNC	HGNC:20453. KLK15.
HPA	HPA019802.
MIM	610601. gene.
PharmGKB	PA134977502.
GenAtlas	Search...
Phylogenomic databases
HOVERGEN	Q9H2R5.
OMA	PGEACVV.
Gene expression databases
ArrayExpress	Q9H2R5.
Bgee	Q9H2R5.
CleanEx	HS_KLK15.
Genevestigator	Q9H2R5.
GermOnline	ENSG00000174562. Homo sapiens.
Family and domain databases
InterPro	IPR018114. Peptidase_S1/S6_AS. IPR001254. Peptidase_S1_S6. IPR001314. Peptidase_S1A. [Graphical view]
Pfam	PF00089. Trypsin. 1 hit. [Graphical view]
PRINTS	PR00722. CHYMOTRYPSIN.
SMART	SM00020. Tryp_SPc. 1 hit. [Graphical view]
PROSITE	PS50240. TRYPSIN_DOM. 1 hit. PS00134. TRYPSIN_HIS. 1 hit. PS00135. TRYPSIN_SER. False negative. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	60018.
SOURCE	Search...

Entry information

Entry name

KLK15_HUMAN

Accession

Primary (citable) accession number: Q9H2R5
Secondary accession number(s): A0AUY8 , Q15358, Q9H2R3, Q9H2R4, Q9H2R6, Q9HBG9

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 18, 2001
Last sequence update:	March 1, 2001
Last modified:	November 3, 2009
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.