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Protein

Ras association domain-containing protein 4

Gene

RASSF4

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Potential tumor suppressor. May act as a KRAS effector protein. May promote apoptosis and cell cycle arrest.1 Publication

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Cell cycle

Names & Taxonomyi

Protein namesi
Recommended name:
Ras association domain-containing protein 4
Gene namesi
Name:RASSF4
ORF Names:AD037
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 10

Organism-specific databases

HGNCiHGNC:20793. RASSF4.

Pathology & Biotechi

Keywords - Diseasei

Tumor suppressor

Organism-specific databases

DisGeNETi83937.
OpenTargetsiENSG00000107551.
PharmGKBiPA134951366.

Polymorphism and mutation databases

BioMutaiRASSF4.
DMDMi109892952.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002403981 – 321Ras association domain-containing protein 4Add BLAST321

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei141PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9H2L5.
MaxQBiQ9H2L5.
PaxDbiQ9H2L5.
PeptideAtlasiQ9H2L5.
PRIDEiQ9H2L5.

PTM databases

iPTMnetiQ9H2L5.
PhosphoSitePlusiQ9H2L5.

Expressioni

Tissue specificityi

Widely expressed. Frequently down-regulated in tumor cell lines.1 Publication

Gene expression databases

BgeeiENSG00000107551.
CleanExiHS_RASSF4.
ExpressionAtlasiQ9H2L5. baseline and differential.
GenevisibleiQ9H2L5. HS.

Organism-specific databases

HPAiHPA038834.

Interactioni

Subunit structurei

Interacts directly with activated KRAS in a GTP-dependent manner.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
MAGEA6P433605EBI-2933362,EBI-1045155
STK3Q131888EBI-2933362,EBI-992580
STK4Q130436EBI-2933362,EBI-367376

Protein-protein interaction databases

BioGridi123820. 3 interactors.
IntActiQ9H2L5. 6 interactors.
STRINGi9606.ENSP00000339692.

Structurei

3D structure databases

ProteinModelPortaliQ9H2L5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini174 – 262Ras-associatingPROSITE-ProRule annotationAdd BLAST89
Domaini270 – 317SARAHPROSITE-ProRule annotationAdd BLAST48

Sequence similaritiesi

Contains 1 Ras-associating domain.PROSITE-ProRule annotation
Contains 1 SARAH domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG4239. Eukaryota.
ENOG410Y2N1. LUCA.
GeneTreeiENSGT00510000046426.
HOGENOMiHOG000231738.
HOVERGENiHBG054302.
InParanoidiQ9H2L5.
KOiK09851.
OMAiMFLMEAD.
OrthoDBiEOG091G0BOA.
PhylomeDBiQ9H2L5.
TreeFamiTF319243.

Family and domain databases

InterProiIPR033614. C-RASSF.
IPR000159. RA_dom.
IPR033622. RASSF4.
IPR011524. SARAH_dom.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PANTHERiPTHR22738. PTHR22738. 1 hit.
PTHR22738:SF4. PTHR22738:SF4. 1 hit.
PfamiPF16517. Nore1-SARAH. 1 hit.
PF00788. RA. 1 hit.
[Graphical view]
SMARTiSM00314. RA. 1 hit.
[Graphical view]
SUPFAMiSSF54236. SSF54236. 1 hit.
PROSITEiPS50200. RA. 1 hit.
PS50951. SARAH. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9H2L5-1) [UniParc]FASTAAdd to basket
Also known as: A

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKEDCLPSSH VPISDSKSIQ KSELLGLLKT YNCYHEGKSF QLRHREEEGT
60 70 80 90 100
LIIEGLLNIA WGLRRPIRLQ MQDDREQVHL PSTSWMPRRP SCPLKEPSPQ
110 120 130 140 150
NGNITAQGPS IQPVHKAESS TDSSGPLEEA EEAPQLMRTK SDASCMSQRR
160 170 180 190 200
PKCRAPGEAQ RIRRHRFSIN GHFYNHKTSV FTPAYGSVTN VRVNSTMTTL
210 220 230 240 250
QVLTLLLNKF RVEDGPSEFA LYIVHESGER TKLKDCEYPL ISRILHGPCE
260 270 280 290 300
KIARIFLMEA DLGVEVPHEV AQYIKFEMPV LDSFVEKLKE EEEREIIKLT
310 320
MKFQALRLTM LQRLEQLVEA K
Length:321
Mass (Da):36,748
Last modified:June 27, 2006 - v2
Checksum:i8EB5990165886D99
GO
Isoform 2 (identifier: Q9H2L5-2) [UniParc]FASTAAdd to basket
Also known as: C

The sequence of this isoform differs from the canonical sequence as follows:
     29-46: Missing.
     94-94: L → LGCWSLLLGLSSLSLPAAISALQLSVFR

Show »
Length:330
Mass (Da):37,255
Checksum:i1A4C69ED22754F32
GO
Isoform 3 (identifier: Q9H2L5-3) [UniParc]FASTAAdd to basket
Also known as: B

The sequence of this isoform differs from the canonical sequence as follows:
     1-70: Missing.

Show »
Length:251
Mass (Da):28,689
Checksum:i29DEAD2F42FE7C1E
GO
Isoform 4 (identifier: Q9H2L5-4) [UniParc]FASTAAdd to basket
Also known as: D

The sequence of this isoform differs from the canonical sequence as follows:
     95-126: KEPSPQNGNITAQGPSIQPVHKAESSTDSSGP → APGGGRGGPPADADQERRQLHEPEEAQ
     127-321: Missing.

Show »
Length:121
Mass (Da):13,735
Checksum:i8F3E99BD8313440E
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti107Q → K in AAG44666 (Ref. 1) Curated1
Sequence conflicti107Q → K in AAO61138 (Ref. 2) Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_03443810H → Y.Corresponds to variant rs34692238dbSNPEnsembl.1
Natural variantiVAR_03443988R → G.Corresponds to variant rs870957dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0193551 – 70Missing in isoform 3. 1 PublicationAdd BLAST70
Alternative sequenceiVSP_01935629 – 46Missing in isoform 2. 1 PublicationAdd BLAST18
Alternative sequenceiVSP_01935794L → LGCWSLLLGLSSLSLPAAIS ALQLSVFR in isoform 2. 1 Publication1
Alternative sequenceiVSP_01935895 – 126KEPSP…DSSGP → APGGGRGGPPADADQERRQL HEPEEAQ in isoform 4. 1 PublicationAdd BLAST32
Alternative sequenceiVSP_019359127 – 321Missing in isoform 4. 1 PublicationAdd BLAST195

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY216713 mRNA. Translation: AAO61138.1.
AY216714 mRNA. Translation: AAO61139.1.
AY216715 mRNA. Translation: AAO61140.1.
AY216716 mRNA. Translation: AAO61141.1.
AF260335 mRNA. Translation: AAG44666.1.
AL353801 Genomic DNA. Translation: CAI13063.1.
BC032593 mRNA. Translation: AAH32593.1.
AL713716 mRNA. Translation: CAD28511.1.
CCDSiCCDS7208.1. [Q9H2L5-1]
RefSeqiNP_114412.2. NM_032023.3. [Q9H2L5-1]
UniGeneiHs.522895.

Genome annotation databases

EnsembliENST00000340258; ENSP00000339692; ENSG00000107551. [Q9H2L5-1]
GeneIDi83937.
KEGGihsa:83937.
UCSCiuc001jbo.4. human. [Q9H2L5-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY216713 mRNA. Translation: AAO61138.1.
AY216714 mRNA. Translation: AAO61139.1.
AY216715 mRNA. Translation: AAO61140.1.
AY216716 mRNA. Translation: AAO61141.1.
AF260335 mRNA. Translation: AAG44666.1.
AL353801 Genomic DNA. Translation: CAI13063.1.
BC032593 mRNA. Translation: AAH32593.1.
AL713716 mRNA. Translation: CAD28511.1.
CCDSiCCDS7208.1. [Q9H2L5-1]
RefSeqiNP_114412.2. NM_032023.3. [Q9H2L5-1]
UniGeneiHs.522895.

3D structure databases

ProteinModelPortaliQ9H2L5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi123820. 3 interactors.
IntActiQ9H2L5. 6 interactors.
STRINGi9606.ENSP00000339692.

PTM databases

iPTMnetiQ9H2L5.
PhosphoSitePlusiQ9H2L5.

Polymorphism and mutation databases

BioMutaiRASSF4.
DMDMi109892952.

Proteomic databases

EPDiQ9H2L5.
MaxQBiQ9H2L5.
PaxDbiQ9H2L5.
PeptideAtlasiQ9H2L5.
PRIDEiQ9H2L5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000340258; ENSP00000339692; ENSG00000107551. [Q9H2L5-1]
GeneIDi83937.
KEGGihsa:83937.
UCSCiuc001jbo.4. human. [Q9H2L5-1]

Organism-specific databases

CTDi83937.
DisGeNETi83937.
GeneCardsiRASSF4.
HGNCiHGNC:20793. RASSF4.
HPAiHPA038834.
MIMi610559. gene.
neXtProtiNX_Q9H2L5.
OpenTargetsiENSG00000107551.
PharmGKBiPA134951366.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG4239. Eukaryota.
ENOG410Y2N1. LUCA.
GeneTreeiENSGT00510000046426.
HOGENOMiHOG000231738.
HOVERGENiHBG054302.
InParanoidiQ9H2L5.
KOiK09851.
OMAiMFLMEAD.
OrthoDBiEOG091G0BOA.
PhylomeDBiQ9H2L5.
TreeFamiTF319243.

Miscellaneous databases

ChiTaRSiRASSF4. human.
GenomeRNAii83937.
PROiQ9H2L5.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000107551.
CleanExiHS_RASSF4.
ExpressionAtlasiQ9H2L5. baseline and differential.
GenevisibleiQ9H2L5. HS.

Family and domain databases

InterProiIPR033614. C-RASSF.
IPR000159. RA_dom.
IPR033622. RASSF4.
IPR011524. SARAH_dom.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PANTHERiPTHR22738. PTHR22738. 1 hit.
PTHR22738:SF4. PTHR22738:SF4. 1 hit.
PfamiPF16517. Nore1-SARAH. 1 hit.
PF00788. RA. 1 hit.
[Graphical view]
SMARTiSM00314. RA. 1 hit.
[Graphical view]
SUPFAMiSSF54236. SSF54236. 1 hit.
PROSITEiPS50200. RA. 1 hit.
PS50951. SARAH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRASF4_HUMAN
AccessioniPrimary (citable) accession number: Q9H2L5
Secondary accession number(s): Q86WH5
, Q86WH6, Q86WH7, Q8N5A9, Q8TCK6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 27, 2006
Last sequence update: June 27, 2006
Last modified: November 30, 2016
This is version 104 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 10
    Human chromosome 10: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.