Q9H2G9 (GO45_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Golgin-45

Alternative name(s):
Basic leucine zipper nuclear factor 1
JEM-1
p45 basic leucine-zipper nuclear factor

Gene names

Name:	BLZF1
Synonyms:	JEM1

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	400 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required for normal Golgi structure and for protein transport from the endoplasmic reticulum (ER) through the Golgi apparatus to the cell surface.
Subunit structure	Interacts with GORASP2 and with the GTP-bound form of RAB2, but not with other Golgi Rab proteins. GORASP2 and BLZF1 form a RAB2 effector complex on medial Golgi. Ref.3
Subcellular location	Golgi apparatus lumen Ref.1 Ref.2 Ref.3. Isoform 1: Nucleus Ref.1 Ref.2 Ref.3. Isoform 2: Cytoplasm Ref.1 Ref.2 Ref.3.
Tissue specificity	Ubiquitous. Also found in cell lines derived from several hematopoietic pathologies, such as T-cell leukemia, pro-B, pre-B, myeloma, and plasmacytoma cell lines, but not in Burkitt lymphoma cells. Ref.1 Ref.2 Ref.3
Induction	Up-regulated by retinoids. Ref.1 Ref.2
Domain	The tankyrase-binding motif (also named TBD) is required for interaction with tankyrase TNKS and TNKS2. Ref.9
Post-translational modification	ADP-ribosylated by tankyrase TNKS and TNKS2. Poly-ADP-ribosylated protein is recognized by RNF146, followed by ubiquitination. Ubiquitinated by RNF146 when poly-ADP-ribosylated, leading to its degradation. Ref.9
Caution	Because of the presence of a potential basic motif and leucine-zipper domain, Ref.1 and Ref.2 have thought that BLZF1 is a potential transcription factor. They found it localized in the nucleus, except isoform 2, which was cytoplasmic. However, homology at several typical position for basic or hydrophobic residues is missing.

Ontologies

Keywords
Biological process	ER-Golgi transport Protein transport Transport
Cellular component	Cytoplasm Golgi apparatus Nucleus
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Coiled coil
PTM	ADP-ribosylation Phosphoprotein Ubl conjugation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	Golgi organization Inferred from direct assay Ref.3. Source: MGI Golgi to plasma membrane protein transport Inferred from direct assay Ref.3. Source: MGI cell proliferation Traceable author statement Ref.1. Source: ProtInc regulation of cell growth Non-traceable author statement Ref.1. Source: UniProtKB regulation of transcription from RNA polymerase II promoter Traceable author statement Ref.1. Source: ProtInc
Cellular_component	Golgi lumen Inferred from electronic annotation. Source: UniProtKB-SubCell Golgi membrane Inferred from electronic annotation. Source: Compara cytoplasm Non-traceable author statement Ref.2. Source: UniProtKB nucleus Non-traceable author statement Ref.1. Source: UniProtKB
Molecular_function	DNA binding Non-traceable author statement Ref.1. Source: UniProtKB sequence-specific DNA binding transcription factor activity Non-traceable author statement Ref.1. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9H2G9-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9H2G9-2)

Also known as: JEM1s;

The sequence of this isoform differs from the canonical sequence as follows:
     158-174: NRELKKLLVASVGDDLQ → RRALIDKMSTTLYVWTF
     175-400: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 400	400	Golgin-45		PRO_0000087539
Regions
	Region	394 – 400	7	Essential for the interaction with GORASP2
	Coiled coil	120 – 213	94	Potential
	Motif	18 – 22	5	Tankyrase-binding motif
Amino acid modifications
	Modified residue	49	1	Phosphoserine Ref.8
	Modified residue	348	1	Phosphothreonine Ref.7
	Modified residue	353	1	Phosphoserine Ref.7
Natural variations
	Alternative sequence	158 – 174	17	NRELK…GDDLQ → RRALIDKMSTTLYVWTF in isoform 2.		VSP_011186
	Alternative sequence	175 – 400	226	Missing in isoform 2.		VSP_011187
	Natural variant	40	1	Q → R. Corresponds to variant rs1028180 [ dbSNP | Ensembl ].		VAR_028142
	Natural variant	196	1	R → Q. Corresponds to variant rs1064274 [ dbSNP | Ensembl ].		VAR_028143
Experimental info
	Sequence conflict	368	1	A → V in AAG37822. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 17, 2006. Version 2. Checksum: F3F9DA9B13DF45B5	FASTA	400	44,910
	10 20 30 40 50 60 MTTKNLETKV TVTSSPIRGA GDGMETEEPP KSVEVTSGVQ SRKHHSLQSP WKKAVPSESP 70 80 90 100 110 120 GVLQLGKMLT EKAMEVKAVR ILVPKAAITH DIPNKNTKVK SLGHHKGEFL GQSEGVIEPN 130 140 150 160 170 180 KELSEVKNVL EKLKNSERRL LQDKEGLSNQ LRVQTEVNRE LKKLLVASVG DDLQYHFERL 190 200 210 220 230 240 AREKNQLILE NEALGRNTAQ LSEQLERMSI QCDVWRSKFL ASRVMADELT NSRAALQRQN 250 260 270 280 290 300 RDAHGAIQDL LSEREQFRQE MIATQKLLEE LLVSLQWGRE QTYSPSVQPH STAELALTNH 310 320 330 340 350 360 KLAKAVNSHL LGNVGINNQK KIPSTVEFCS TPAEKMAETV LRILDPVTCK ESSPDNPFFE 370 380 390 400 SSPTTLLATK KNIGRFHPYT RYENITFNCC NHCRGELIAL
	Isoform 2 (JEM1s) [UniParc]. Checksum: 42524E04C956A3AB	FASTA	174	19,362
	10 20 30 40 50 60 MTTKNLETKV TVTSSPIRGA GDGMETEEPP KSVEVTSGVQ SRKHHSLQSP WKKAVPSESP 70 80 90 100 110 120 GVLQLGKMLT EKAMEVKAVR ILVPKAAITH DIPNKNTKVK SLGHHKGEFL GQSEGVIEPN 130 140 150 160 170 KELSEVKNVL EKLKNSERRL LQDKEGLSNQ LRVQTEVRRA LIDKMSTTLY VWTF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"JEM-1, a novel gene encoding a leucine-zipper nuclear factor upregulated during retinoid-induced maturation of NB4 promyelocytic leukaemia." Duprez E., Tong J.-H., Derre J., Chen S.-J., Berger R., Chen Z., Lanotte M. Oncogene 14:1563-1570(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INDUCTION.
[2]	"Genomic organization of the JEM-1 (BLZF1) gene on human chromosome 1q24: molecular cloning and analysis of its promoter region." Tong J.-H., Fant X., Benoit G., Chen S.-J., Chen Z., Lanotte M. Genomics 69:380-390(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORMS 1 AND 2), SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INDUCTION.
[3]	"A GRASP55-rab2 effector complex linking Golgi structure to membrane traffic." Short B., Preisinger C., Koerner R., Kopajtich R., Byron O., Barr F.A. J. Cell Biol. 155:877-883(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH GORASP2 AND THE GTP FORM OF RAB2, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, IDENTIFICATION BY MASS SPECTROMETRY. Tissue: Testis.
[4]	"The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R. Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Liver.
[7]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-348 AND SER-353, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[8]	"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis." Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M. Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-49, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[9]	"RNF146 is a poly(ADP-ribose)-directed E3 ligase that regulates axin degradation and Wnt signalling." Zhang Y., Liu S., Mickanin C., Feng Y., Charlat O., Michaud G.A., Schirle M., Shi X., Hild M., Bauer A., Myer V.E., Finan P.M., Porter J.A., Huang S.M., Cong F. Nat. Cell Biol. 13:623-629(2011) [PubMed] [Europe PMC] [Abstract] Cited for: ADP-RIBOSYLATION, UBIQUITINATION, DOMAIN TANKYRASE-BINDING MOTIF.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U79751 mRNA. Translation: AAB63110.1. AF288161 mRNA. Translation: AAG37835.1. AF273875, AF288162 Genomic DNA. Translation: AAG37821.1. AF272386 , AF272387, AF272388, AF273875, AF288162 Genomic DNA. Translation: AAG37822.1. AL356852 Genomic DNA. Translation: CAI19240.1. AL356852 Genomic DNA. Translation: CAI19242.1. CH471067 Genomic DNA. Translation: EAW90837.1. BC020716 mRNA. Translation: AAH20716.1.
IPI	IPI00386502. IPI00455985.
RefSeq	NP_003657.1. NM_003666.2.
UniGene	Hs.130746.
3D structure databases
ProteinModelPortal	Q9H2G9.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9H2G9. 4 interactions.
STRING	9606.ENSP00000327541.
PTM databases
PhosphoSite	Q9H2G9.
Polymorphism databases
DMDM	116242501.
Proteomic databases
PaxDb	Q9H2G9.
PRIDE	Q9H2G9.
Protocols and materials databases
DNASU	8548.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000329281; ENSP00000327541; ENSG00000117475. ENST00000367807; ENSP00000356781; ENSG00000117475. ENST00000367808; ENSP00000356782; ENSG00000117475.
GeneID	8548.
KEGG	hsa:8548.
UCSC	uc001gfw.3. human. uc001gfx.2. human.
Organism-specific databases
CTD	8548.
GeneCards	GC01P169337.
HGNC	HGNC:1065. BLZF1.
HPA	HPA025703. HPA027331.
MIM	608692. gene.
neXtProt	NX_Q9H2G9.
PharmGKB	PA25375.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG279660.
HOGENOM	HOG000088649.
HOVERGEN	HBG051750.
InParanoid	Q9H2G9.
OMA	QFRQEMI.
OrthoDB	EOG43R3N2.
PhylomeDB	Q9H2G9.
Enzyme and pathway databases
Reactome	REACT_115566. Cell Cycle. REACT_21300. Mitotic M-M/G1 phases.
Gene expression databases
ArrayExpress	Q9H2G9.
Bgee	Q9H2G9.
CleanEx	HS_BLZF1.
Genevestigator	Q9H2G9.
GermOnline	ENSG00000117475. Homo sapiens.
Family and domain databases
InterPro	IPR027095. Golgin-45. IPR013183. Hsk3_like. [Graphical view]
PANTHER	PTHR13066. PTHR13066. 1 hit.
Pfam	PF08227. DASH_Hsk3. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	8548.
NextBio	32028.
SOURCE	Search...

Entry information

Entry name

GO45_HUMAN

Accession

Primary (citable) accession number: Q9H2G9
Secondary accession number(s): O15298 , Q5T531, Q5T533, Q9GZX4

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2004
Last sequence update:	October 17, 2006
Last modified:	April 3, 2013
This is version 90 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.