Q9H2G4 (TSYL2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Testis-specific Y-encoded-like protein 2
Short name=TSPY-like protein 2

Alternative name(s):
Cell division autoantigen 1
Cutaneous T-cell lymphoma-associated antigen se20-4
Short name=CTCL-associated antigen se20-4
Differentially-expressed nucleolar TGF-beta1 target protein
Nuclear protein of 79 kDa
Short name=NP79

Gene names

Name:	TSPYL2
Synonyms:	CDA1, DENTT, TSPX
ORF Names:	HRIHFB2216

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	693 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Part of the CASK/TRB1/TSPYL2 transcriptional complex which modulates gene expression in response to neuronal synaptic activity, probably by facilitating nucleosome assembly. May inhibit cell proliferation by inducing p53-dependent CDKN1A expression. Ref.1 Ref.12
Subunit structure	Interacts with histones. Interacts with CASK. Part of a complex containing CASK, TRB1 and TSPYL2 By similarity.
Subcellular location	Nucleus. Cytoplasm By similarity. Note: Enriched in transcriptionally active regions of chromatin in neurons By similarity. Ref.1 Ref.6
Tissue specificity	Ubiquitously expressed, with highest levels in brain, testis and heart, and lowest levels in liver and pancreas. Ref.2 Ref.6 Ref.8 Ref.9
Induction	Up-regulated in growth-arrested T-cells. Induced by TGFB1 and all-trans retinoic acid (ATRA) in lung cancer cells (at protein level). Ref.6 Ref.10 Ref.11
Post-translational modification	Phosphorylation at Ser-20 and/or Thr-340 impairs function on cell proliferation Probable.
Miscellaneous	Synaptic activity down-regulates TSPYL2 protein levels by inducing rapid proteasomal degradation By similarity. Subject to X inactivation.
Sequence similarities	Belongs to the nucleosome assembly protein (NAP) family.
Sequence caution	The sequence AAG53596.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Cell cycle Transcription Transcription regulation
Cellular component	Cytoplasm Nucleus
Molecular function	Chromatin regulator
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	activation of signaling protein activity involved in unfolded protein response Traceable author statement. Source: Reactome cell cycle Inferred from electronic annotation. Source: UniProtKB-KW chromatin modification Inferred from electronic annotation. Source: UniProtKB-KW negative regulation of DNA replication Inferred from direct assay Ref.1. Source: UniProtKB negative regulation of cell cycle Non-traceable author statement Ref.1. Source: UniProtKB negative regulation of cell growth Inferred from direct assay Ref.1. Source: UniProtKB nucleosome assembly Inferred from electronic annotation. Source: InterPro regulation of signal transduction Non-traceable author statement Ref.1. Source: UniProtKB regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	centrosome Inferred from direct assay. Source: HPA cytoplasm Inferred from direct assay. Source: HPA nucleolus Inferred from direct assay Ref.1. Source: UniProtKB nucleoplasm Traceable author statement. Source: Reactome plasma membrane Inferred from direct assay. Source: HPA
Molecular_function	rDNA binding Non-traceable author statement Ref.1. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 693	693	Testis-specific Y-encoded-like protein 2		PRO_0000289100
Regions
	Compositional bias	4 – 75	72	Pro-rich
	Compositional bias	180 – 211	32	Arg-rich
	Compositional bias	473 – 542	70	Asn-rich
	Compositional bias	557 – 675	119	Asp-rich
Amino acid modifications
	Modified residue	18	1	Phosphoserine Ref.13
	Modified residue	20	1	Phosphoserine Ref.1
	Modified residue	340	1	Phosphothreonine Probable
Experimental info
	Mutagenesis	20	1	S → A: Impairs effect on cell proliferation; when associated with A-340. Ref.1
	Mutagenesis	340	1	T → A: Impairs effect on cell proliferation; when associated with A-20. Ref.1
	Sequence conflict	442	1	D → N in AAG53596. Ref.6
	Sequence conflict	566	1	D → V in AAG53596. Ref.6
	Sequence conflict	609	1	V → G in BAA34802. Ref.7
	Sequence conflict	615	1	K → R in BAA34802. Ref.7

Sequences

	Sequence		Length	Mass (Da)
	Q9H2G4 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: 726F3BDC10A36165	FASTA	693	79,435
	10 20 30 40 50 60 MDRPDEGPPA KTRRLSSSES PQRDPPPPPP PPPLLRLPLP PPQQRPRLQE ETEAAQVLAD 70 80 90 100 110 120 MRGVGLGPAL PPPPPYVILE EGGIRAYFTL GAECPGWDST IESGYGEAPP PTESLEALPT 130 140 150 160 170 180 PEASGGSLEI DFQVVQSSSF GGEGALETCS AVGWAPQRLV DPKSKEEAII IVEDEDEDER 190 200 210 220 230 240 ESMRSSRRRR RRRRRKQRKV KRESRERNAE RMESILQALE DIQLDLEAVN IKAGKAFLRL 250 260 270 280 290 300 KRKFIQMRRP FLERRDLIIQ HIPGFWVKAF LNHPRISILI NRRDEDIFRY LTNLQVQDLR 310 320 330 340 350 360 HISMGYKMKL YFQTNPYFTN MVIVKEFQRN RSGRLVSHST PIRWHRGQEP QARRHGNQDA 370 380 390 400 410 420 SHSFFSWFSN HSLPEADRIA EIIKNDLWVN PLRYYLRERG SRIKRKKQEM KKRKTRGRCE 430 440 450 460 470 480 VVIMEDAPDY YAVEDIFSEI SDIDETIHDI KISDFMETTD YFETTDNEIT DINENICDSE 490 500 510 520 530 540 NPDHNEVPNN ETTDNNESAD DHETTDNNES ADDNNENPED NNKNTDDNEE NPNNNENTYG 550 560 570 580 590 600 NNFFKGGFWG SHGNNQDSSD SDNEADEASD DEDNDGNEGD NEGSDDDGNE GDNEGSDDDD 610 620 630 640 650 660 RDIEYYEKVI EDFDKDQADY EDVIEIISDE SVEEEGIEEG IQQDEDIYEE GNYEEEGSED 670 680 690 VWEEGEDSDD SDLEDVLQVP NGWANPGKRG KTG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"SET-related cell division autoantigen-1 (CDA1) arrests cell growth." Chai Z., Sarcevic B., Mawson A., Toh B.-H. J. Biol. Chem. 276:33665-33674(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, PHOSPHORYLATION AT SER-20 AND THR-340, MUTAGENESIS OF SER-20 AND THR-340, FUNCTION. Tissue: Testis.
[2]	"Serological detection of cutaneous T-cell lymphoma-associated antigens." Eichmueller S., Usener D., Dummer R., Stein A., Thiel D., Schadendorf D. Proc. Natl. Acad. Sci. U.S.A. 98:629-634(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY. Tissue: Testis.
[3]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Amygdala.
[4]	"The DNA sequence of the human X chromosome." Ross M.T., Grafham D.V., Coffey A.J., Scherer S., McLay K., Muzny D., Platzer M., Howell G.R., Burrows C., Bird C.P., Frankish A., Lovell F.L., Howe K.L., Ashurst J.L., Fulton R.S., Sudbrak R., Wen G., Jones M.C. , Hurles M.E., Andrews T.D., Scott C.E., Searle S., Ramser J., Whittaker A., Deadman R., Carter N.P., Hunt S.E., Chen R., Cree A., Gunaratne P., Havlak P., Hodgson A., Metzker M.L., Richards S., Scott G., Steffen D., Sodergren E., Wheeler D.A., Worley K.C., Ainscough R., Ambrose K.D., Ansari-Lari M.A., Aradhya S., Ashwell R.I., Babbage A.K., Bagguley C.L., Ballabio A., Banerjee R., Barker G.E., Barlow K.F., Barrett I.P., Bates K.N., Beare D.M., Beasley H., Beasley O., Beck A., Bethel G., Blechschmidt K., Brady N., Bray-Allen S., Bridgeman A.M., Brown A.J., Brown M.J., Bonnin D., Bruford E.A., Buhay C., Burch P., Burford D., Burgess J., Burrill W., Burton J., Bye J.M., Carder C., Carrel L., Chako J., Chapman J.C., Chavez D., Chen E., Chen G., Chen Y., Chen Z., Chinault C., Ciccodicola A., Clark S.Y., Clarke G., Clee C.M., Clegg S., Clerc-Blankenburg K., Clifford K., Cobley V., Cole C.G., Conquer J.S., Corby N., Connor R.E., David R., Davies J., Davis C., Davis J., Delgado O., Deshazo D., Dhami P., Ding Y., Dinh H., Dodsworth S., Draper H., Dugan-Rocha S., Dunham A., Dunn M., Durbin K.J., Dutta I., Eades T., Ellwood M., Emery-Cohen A., Errington H., Evans K.L., Faulkner L., Francis F., Frankland J., Fraser A.E., Galgoczy P., Gilbert J., Gill R., Gloeckner G., Gregory S.G., Gribble S., Griffiths C., Grocock R., Gu Y., Gwilliam R., Hamilton C., Hart E.A., Hawes A., Heath P.D., Heitmann K., Hennig S., Hernandez J., Hinzmann B., Ho S., Hoffs M., Howden P.J., Huckle E.J., Hume J., Hunt P.J., Hunt A.R., Isherwood J., Jacob L., Johnson D., Jones S., de Jong P.J., Joseph S.S., Keenan S., Kelly S., Kershaw J.K., Khan Z., Kioschis P., Klages S., Knights A.J., Kosiura A., Kovar-Smith C., Laird G.K., Langford C., Lawlor S., Leversha M., Lewis L., Liu W., Lloyd C., Lloyd D.M., Loulseged H., Loveland J.E., Lovell J.D., Lozado R., Lu J., Lyne R., Ma J., Maheshwari M., Matthews L.H., McDowall J., McLaren S., McMurray A., Meidl P., Meitinger T., Milne S., Miner G., Mistry S.L., Morgan M., Morris S., Mueller I., Mullikin J.C., Nguyen N., Nordsiek G., Nyakatura G., O'dell C.N., Okwuonu G., Palmer S., Pandian R., Parker D., Parrish J., Pasternak S., Patel D., Pearce A.V., Pearson D.M., Pelan S.E., Perez L., Porter K.M., Ramsey Y., Reichwald K., Rhodes S., Ridler K.A., Schlessinger D., Schueler M.G., Sehra H.K., Shaw-Smith C., Shen H., Sheridan E.M., Shownkeen R., Skuce C.D., Smith M.L., Sotheran E.C., Steingruber H.E., Steward C.A., Storey R., Swann R.M., Swarbreck D., Tabor P.E., Taudien S., Taylor T., Teague B., Thomas K., Thorpe A., Timms K., Tracey A., Trevanion S., Tromans A.C., d'Urso M., Verduzco D., Villasana D., Waldron L., Wall M., Wang Q., Warren J., Warry G.L., Wei X., West A., Whitehead S.L., Whiteley M.N., Wilkinson J.E., Willey D.L., Williams G., Williams L., Williamson A., Williamson H., Wilming L., Woodmansey R.L., Wray P.W., Yen J., Zhang J., Zhou J., Zoghbi H., Zorilla S., Buck D., Reinhardt R., Poustka A., Rosenthal A., Lehrach H., Meindl A., Minx P.J., Hillier L.W., Willard H.F., Wilson R.K., Waterston R.H., Rice C.M., Vaudin M., Coulson A., Nelson D.L., Weinstock G., Sulston J.E., Durbin R.M., Hubbard T., Gibbs R.A., Beck S., Rogers J., Bentley D.R. Nature 434:325-337(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Lung and Placenta.
[6]	"Identification of Differentially expressed nucleolar TGF-beta1 target (DENTT) in human lung cancer cells that is a new member of the TSPY/SET/NAP-1 superfamily." Ozbun L.L., You L., Kiang S., Angdisen J., Martinez A., Jakowlew S.B. Genomics 73:179-193(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 42-693, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, INDUCTION BY TGFB1.
[7]	"Selection system for genes encoding nuclear-targeted proteins." Ueki N., Oda T., Kondo M., Yano K., Noguchi T., Muramatsu M.-A. Nat. Biotechnol. 16:1338-1342(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 208-693. Tissue: Fetal brain.
[8]	"Isolation of differentially expressed genes in human heart tissues." Sun G., Yuen Chan S., Yuan Y., Wang Chan K., Qiu G., Sun K., Ping Leung M. Biochim. Biophys. Acta 1588:241-246(2002) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION, TISSUE SPECIFICITY.
[9]	"TSPY, the candidate gonadoblastoma gene on the human Y chromosome, has a widely expressed homologue on the X -- implications for Y chromosome evolution." Delbridge M.L., Longepied G., Depetris D., Mattei M.-G., Disteche C.M., Marshall Graves J.A., Mitchell M.J. Chromosome Res. 12:345-356(2004) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION, TISSUE SPECIFICITY.
[10]	"A constitutively active arylhydrocarbon receptor induces growth inhibition of jurkat T cells through changes in the expression of genes related to apoptosis and cell cycle arrest." Ito T., Tsukumo S., Suzuki N., Motohashi H., Yamamoto M., Fujii-Kuriyama Y., Mimura J., Lin T.-M., Peterson R.E., Tohyama C., Nohara K. J. Biol. Chem. 279:25204-25210(2004) [PubMed] [Europe PMC] [Abstract] Cited for: INDUCTION.
[11]	"Differentially expressed nucleolar TGF-beta1 target (DENTT) shows tissue-specific nuclear and cytoplasmic localization and increases TGF-beta1-responsive transcription in primates." Ozbun L.L., Martinez A., Jakowlew S.B. Biochim. Biophys. Acta 1728:163-180(2005) [PubMed] [Europe PMC] [Abstract] Cited for: INDUCTION.
[12]	"Antiproliferative autoantigen CDA1 transcriptionally up-regulates p21(Waf1/Cip1) by activating p53 and MEK/ERK1/2 MAPK pathways." Tu Y., Wu W., Wu T., Cao Z., Wilkins R., Toh B.-H., Cooper M.E., Chai Z. J. Biol. Chem. 282:11722-11731(2007) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[13]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-18, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[14]	"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis." Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M. Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Cervix carcinoma.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF273046 mRNA. Translation: AAG34906.1. AY040871 mRNA. Translation: AAK72407.1. AL713652 mRNA. Translation: CAD28461.1. BX322635 Genomic DNA. Translation: CAI42531.1. BC001566 mRNA. Translation: AAH01566.1. BC024270 mRNA. Translation: AAH24270.1. AF254794 mRNA. Translation: AAG53596.1. Different initiation. AB015345 mRNA. Translation: BAA34802.1.
IPI	IPI00005465.
RefSeq	NP_071400.1. NM_022117.3.
UniGene	Hs.136164. Hs.592916.
3D structure databases
ProteinModelPortal	Q9H2G4.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9H2G4. 8 interactions.
MINT	MINT-4719361.
STRING	9606.ENSP00000364591.
PTM databases
PhosphoSite	Q9H2G4.
Polymorphism databases
DMDM	74752604.
Proteomic databases
PaxDb	Q9H2G4.
PRIDE	Q9H2G4.
Protocols and materials databases
DNASU	64061.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000375442; ENSP00000364591; ENSG00000184205. ENST00000598656; ENSP00000469414; ENSG00000268269.
GeneID	64061.
KEGG	hsa:64061.
UCSC	uc004drw.3. human.
Organism-specific databases
CTD	64061.
GeneCards	GC0XP053111.
HGNC	HGNC:24358. TSPYL2.
HPA	HPA044133.
MIM	300564. gene.
neXtProt	NX_Q9H2G4.
PharmGKB	PA134930188.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG295286.
HOGENOM	HOG000154699.
HOVERGEN	HBG014779.
InParanoid	Q9H2G4.
KO	K11285.
OMA	DYFETTD.
OrthoDB	EOG4894MQ.
PhylomeDB	Q9H2G4.
Enzyme and pathway databases
Reactome	REACT_116125. Disease.
Gene expression databases
Bgee	Q9H2G4.
CleanEx	HS_TSPYL2.
Genevestigator	Q9H2G4.
Family and domain databases
InterPro	IPR002164. NAP_family. [Graphical view]
PANTHER	PTHR11875. PTHR11875. 1 hit.
Pfam	PF00956. NAP. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	TSPYL2. human.
GenomeRNAi	64061.
NextBio	65816.
SOURCE	Search...

Entry information

Entry name

TSYL2_HUMAN

Accession

Primary (citable) accession number: Q9H2G4
Secondary accession number(s): O94799, Q96DG7, Q9BZW6

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 29, 2007
Last sequence update:	March 1, 2001
Last modified:	May 1, 2013
This is version 97 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.