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Q9H2G4 (TSYL2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 106. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Testis-specific Y-encoded-like protein 2

Short name=TSPY-like protein 2
Alternative name(s):
Cell division autoantigen 1
Cutaneous T-cell lymphoma-associated antigen se20-4
Short name=CTCL-associated antigen se20-4
Differentially-expressed nucleolar TGF-beta1 target protein
Nuclear protein of 79 kDa
Short name=NP79
Gene names
Name:TSPYL2
Synonyms:CDA1, DENTT, TSPX
ORF Names:HRIHFB2216
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length693 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Part of the CASK/TBR1/TSPYL2 transcriptional complex which modulates gene expression in response to neuronal synaptic activity, probably by facilitating nucleosome assembly. May inhibit cell proliferation by inducing p53-dependent CDKN1A expression. Ref.1 Ref.12

Subunit structure

Interacts with histones. Interacts with CASK. Part of a complex containing CASK, TBR1 and TSPYL2 By similarity.

Subcellular location

Nucleus. Cytoplasm By similarity. Note: Enriched in transcriptionally active regions of chromatin in neurons By similarity. Ref.1 Ref.6

Tissue specificity

Ubiquitously expressed, with highest levels in brain, testis and heart, and lowest levels in liver and pancreas. Ref.2 Ref.6 Ref.8 Ref.9

Induction

Up-regulated in growth-arrested T-cells. Induced by TGFB1 and all-trans retinoic acid (ATRA) in lung cancer cells (at protein level). Ref.6 Ref.10 Ref.11

Post-translational modification

Phosphorylation at Ser-20 and/or Thr-340 impairs function on cell proliferation Probable.

Miscellaneous

Synaptic activity down-regulates TSPYL2 protein levels by inducing rapid proteasomal degradation By similarity.

Subject to X inactivation.

Sequence similarities

Belongs to the nucleosome assembly protein (NAP) family.

Sequence caution

The sequence AAG53596.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Biological processCell cycle
Transcription
Transcription regulation
   Cellular componentCytoplasm
Nucleus
   Molecular functionChromatin regulator
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processactivation of signaling protein activity involved in unfolded protein response

Traceable author statement. Source: Reactome

cell cycle

Inferred from electronic annotation. Source: UniProtKB-KW

cellular protein metabolic process

Traceable author statement. Source: Reactome

chromatin modification

Inferred from electronic annotation. Source: UniProtKB-KW

endoplasmic reticulum unfolded protein response

Traceable author statement. Source: Reactome

negative regulation of DNA replication

Inferred from direct assay Ref.1. Source: UniProtKB

negative regulation of cell cycle

Non-traceable author statement Ref.1. Source: UniProtKB

negative regulation of cell growth

Inferred from direct assay Ref.1. Source: UniProtKB

nucleosome assembly

Inferred from electronic annotation. Source: InterPro

regulation of protein kinase activity

Inferred from direct assay Ref.1. Source: UniProtKB

regulation of signal transduction

Non-traceable author statement Ref.1. Source: UniProtKB

regulation of transcription, DNA-templated

Inferred from electronic annotation. Source: UniProtKB-KW

transcription, DNA-templated

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentcentrosome

Inferred from direct assay. Source: HPA

cytoplasm

Inferred from direct assay. Source: HPA

nucleolus

Inferred from direct assay Ref.1. Source: UniProtKB

nucleoplasm

Traceable author statement. Source: Reactome

nucleus

Inferred from direct assay Ref.1. Source: UniProtKB

plasma membrane

Inferred from direct assay. Source: HPA

   Molecular_functionrDNA binding

Non-traceable author statement Ref.1. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 693693Testis-specific Y-encoded-like protein 2
PRO_0000289100

Regions

Compositional bias4 – 7572Pro-rich
Compositional bias180 – 21132Arg-rich
Compositional bias473 – 54270Asn-rich
Compositional bias557 – 675119Asp-rich

Amino acid modifications

Modified residue181Phosphoserine Ref.13
Modified residue201Phosphoserine Ref.1
Modified residue3401Phosphothreonine Probable

Experimental info

Mutagenesis201S → A: Impairs effect on cell proliferation; when associated with A-340. Ref.1
Mutagenesis3401T → A: Impairs effect on cell proliferation; when associated with A-20. Ref.1
Sequence conflict4421D → N in AAG53596. Ref.6
Sequence conflict5661D → V in AAG53596. Ref.6
Sequence conflict6091V → G in BAA34802. Ref.7
Sequence conflict6151K → R in BAA34802. Ref.7

Sequences

Sequence LengthMass (Da)Tools
Q9H2G4 [UniParc].

Last modified March 1, 2001. Version 1.
Checksum: 726F3BDC10A36165

FASTA69379,435
        10         20         30         40         50         60 
MDRPDEGPPA KTRRLSSSES PQRDPPPPPP PPPLLRLPLP PPQQRPRLQE ETEAAQVLAD 

        70         80         90        100        110        120 
MRGVGLGPAL PPPPPYVILE EGGIRAYFTL GAECPGWDST IESGYGEAPP PTESLEALPT 

       130        140        150        160        170        180 
PEASGGSLEI DFQVVQSSSF GGEGALETCS AVGWAPQRLV DPKSKEEAII IVEDEDEDER 

       190        200        210        220        230        240 
ESMRSSRRRR RRRRRKQRKV KRESRERNAE RMESILQALE DIQLDLEAVN IKAGKAFLRL 

       250        260        270        280        290        300 
KRKFIQMRRP FLERRDLIIQ HIPGFWVKAF LNHPRISILI NRRDEDIFRY LTNLQVQDLR 

       310        320        330        340        350        360 
HISMGYKMKL YFQTNPYFTN MVIVKEFQRN RSGRLVSHST PIRWHRGQEP QARRHGNQDA 

       370        380        390        400        410        420 
SHSFFSWFSN HSLPEADRIA EIIKNDLWVN PLRYYLRERG SRIKRKKQEM KKRKTRGRCE 

       430        440        450        460        470        480 
VVIMEDAPDY YAVEDIFSEI SDIDETIHDI KISDFMETTD YFETTDNEIT DINENICDSE 

       490        500        510        520        530        540 
NPDHNEVPNN ETTDNNESAD DHETTDNNES ADDNNENPED NNKNTDDNEE NPNNNENTYG 

       550        560        570        580        590        600 
NNFFKGGFWG SHGNNQDSSD SDNEADEASD DEDNDGNEGD NEGSDDDGNE GDNEGSDDDD 

       610        620        630        640        650        660 
RDIEYYEKVI EDFDKDQADY EDVIEIISDE SVEEEGIEEG IQQDEDIYEE GNYEEEGSED 

       670        680        690 
VWEEGEDSDD SDLEDVLQVP NGWANPGKRG KTG 

« Hide

References

« Hide 'large scale' references
[1]"SET-related cell division autoantigen-1 (CDA1) arrests cell growth."
Chai Z., Sarcevic B., Mawson A., Toh B.-H.
J. Biol. Chem. 276:33665-33674(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, PHOSPHORYLATION AT SER-20 AND THR-340, MUTAGENESIS OF SER-20 AND THR-340, FUNCTION.
Tissue: Testis.
[2]"Serological detection of cutaneous T-cell lymphoma-associated antigens."
Eichmueller S., Usener D., Dummer R., Stein A., Thiel D., Schadendorf D.
Proc. Natl. Acad. Sci. U.S.A. 98:629-634(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
Tissue: Testis.
[3]"The full-ORF clone resource of the German cDNA consortium."
Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I.
BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Amygdala.
[4]"The DNA sequence of the human X chromosome."
Ross M.T., Grafham D.V., Coffey A.J., Scherer S., McLay K., Muzny D., Platzer M., Howell G.R., Burrows C., Bird C.P., Frankish A., Lovell F.L., Howe K.L., Ashurst J.L., Fulton R.S., Sudbrak R., Wen G., Jones M.C. expand/collapse author list , Hurles M.E., Andrews T.D., Scott C.E., Searle S., Ramser J., Whittaker A., Deadman R., Carter N.P., Hunt S.E., Chen R., Cree A., Gunaratne P., Havlak P., Hodgson A., Metzker M.L., Richards S., Scott G., Steffen D., Sodergren E., Wheeler D.A., Worley K.C., Ainscough R., Ambrose K.D., Ansari-Lari M.A., Aradhya S., Ashwell R.I., Babbage A.K., Bagguley C.L., Ballabio A., Banerjee R., Barker G.E., Barlow K.F., Barrett I.P., Bates K.N., Beare D.M., Beasley H., Beasley O., Beck A., Bethel G., Blechschmidt K., Brady N., Bray-Allen S., Bridgeman A.M., Brown A.J., Brown M.J., Bonnin D., Bruford E.A., Buhay C., Burch P., Burford D., Burgess J., Burrill W., Burton J., Bye J.M., Carder C., Carrel L., Chako J., Chapman J.C., Chavez D., Chen E., Chen G., Chen Y., Chen Z., Chinault C., Ciccodicola A., Clark S.Y., Clarke G., Clee C.M., Clegg S., Clerc-Blankenburg K., Clifford K., Cobley V., Cole C.G., Conquer J.S., Corby N., Connor R.E., David R., Davies J., Davis C., Davis J., Delgado O., Deshazo D., Dhami P., Ding Y., Dinh H., Dodsworth S., Draper H., Dugan-Rocha S., Dunham A., Dunn M., Durbin K.J., Dutta I., Eades T., Ellwood M., Emery-Cohen A., Errington H., Evans K.L., Faulkner L., Francis F., Frankland J., Fraser A.E., Galgoczy P., Gilbert J., Gill R., Gloeckner G., Gregory S.G., Gribble S., Griffiths C., Grocock R., Gu Y., Gwilliam R., Hamilton C., Hart E.A., Hawes A., Heath P.D., Heitmann K., Hennig S., Hernandez J., Hinzmann B., Ho S., Hoffs M., Howden P.J., Huckle E.J., Hume J., Hunt P.J., Hunt A.R., Isherwood J., Jacob L., Johnson D., Jones S., de Jong P.J., Joseph S.S., Keenan S., Kelly S., Kershaw J.K., Khan Z., Kioschis P., Klages S., Knights A.J., Kosiura A., Kovar-Smith C., Laird G.K., Langford C., Lawlor S., Leversha M., Lewis L., Liu W., Lloyd C., Lloyd D.M., Loulseged H., Loveland J.E., Lovell J.D., Lozado R., Lu J., Lyne R., Ma J., Maheshwari M., Matthews L.H., McDowall J., McLaren S., McMurray A., Meidl P., Meitinger T., Milne S., Miner G., Mistry S.L., Morgan M., Morris S., Mueller I., Mullikin J.C., Nguyen N., Nordsiek G., Nyakatura G., O'dell C.N., Okwuonu G., Palmer S., Pandian R., Parker D., Parrish J., Pasternak S., Patel D., Pearce A.V., Pearson D.M., Pelan S.E., Perez L., Porter K.M., Ramsey Y., Reichwald K., Rhodes S., Ridler K.A., Schlessinger D., Schueler M.G., Sehra H.K., Shaw-Smith C., Shen H., Sheridan E.M., Shownkeen R., Skuce C.D., Smith M.L., Sotheran E.C., Steingruber H.E., Steward C.A., Storey R., Swann R.M., Swarbreck D., Tabor P.E., Taudien S., Taylor T., Teague B., Thomas K., Thorpe A., Timms K., Tracey A., Trevanion S., Tromans A.C., d'Urso M., Verduzco D., Villasana D., Waldron L., Wall M., Wang Q., Warren J., Warry G.L., Wei X., West A., Whitehead S.L., Whiteley M.N., Wilkinson J.E., Willey D.L., Williams G., Williams L., Williamson A., Williamson H., Wilming L., Woodmansey R.L., Wray P.W., Yen J., Zhang J., Zhou J., Zoghbi H., Zorilla S., Buck D., Reinhardt R., Poustka A., Rosenthal A., Lehrach H., Meindl A., Minx P.J., Hillier L.W., Willard H.F., Wilson R.K., Waterston R.H., Rice C.M., Vaudin M., Coulson A., Nelson D.L., Weinstock G., Sulston J.E., Durbin R.M., Hubbard T., Gibbs R.A., Beck S., Rogers J., Bentley D.R.
Nature 434:325-337(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Lung and Placenta.
[6]"Identification of Differentially expressed nucleolar TGF-beta1 target (DENTT) in human lung cancer cells that is a new member of the TSPY/SET/NAP-1 superfamily."
Ozbun L.L., You L., Kiang S., Angdisen J., Martinez A., Jakowlew S.B.
Genomics 73:179-193(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 42-693, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, INDUCTION BY TGFB1.
[7]"Selection system for genes encoding nuclear-targeted proteins."
Ueki N., Oda T., Kondo M., Yano K., Noguchi T., Muramatsu M.-A.
Nat. Biotechnol. 16:1338-1342(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 208-693.
Tissue: Fetal brain.
[8]"Isolation of differentially expressed genes in human heart tissues."
Sun G., Yuen Chan S., Yuan Y., Wang Chan K., Qiu G., Sun K., Ping Leung M.
Biochim. Biophys. Acta 1588:241-246(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION, TISSUE SPECIFICITY.
[9]"TSPY, the candidate gonadoblastoma gene on the human Y chromosome, has a widely expressed homologue on the X -- implications for Y chromosome evolution."
Delbridge M.L., Longepied G., Depetris D., Mattei M.-G., Disteche C.M., Marshall Graves J.A., Mitchell M.J.
Chromosome Res. 12:345-356(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION, TISSUE SPECIFICITY.
[10]"A constitutively active arylhydrocarbon receptor induces growth inhibition of jurkat T cells through changes in the expression of genes related to apoptosis and cell cycle arrest."
Ito T., Tsukumo S., Suzuki N., Motohashi H., Yamamoto M., Fujii-Kuriyama Y., Mimura J., Lin T.-M., Peterson R.E., Tohyama C., Nohara K.
J. Biol. Chem. 279:25204-25210(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION.
[11]"Differentially expressed nucleolar TGF-beta1 target (DENTT) shows tissue-specific nuclear and cytoplasmic localization and increases TGF-beta1-responsive transcription in primates."
Ozbun L.L., Martinez A., Jakowlew S.B.
Biochim. Biophys. Acta 1728:163-180(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION.
[12]"Antiproliferative autoantigen CDA1 transcriptionally up-regulates p21(Waf1/Cip1) by activating p53 and MEK/ERK1/2 MAPK pathways."
Tu Y., Wu W., Wu T., Cao Z., Wilkins R., Toh B.-H., Cooper M.E., Chai Z.
J. Biol. Chem. 282:11722-11731(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[13]"A quantitative atlas of mitotic phosphorylation."
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-18, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[14]"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF273046 mRNA. Translation: AAG34906.1.
AY040871 mRNA. Translation: AAK72407.1.
AL713652 mRNA. Translation: CAD28461.1.
BX322635 Genomic DNA. Translation: CAI42531.1.
BC001566 mRNA. Translation: AAH01566.1.
BC024270 mRNA. Translation: AAH24270.1.
AF254794 mRNA. Translation: AAG53596.1. Different initiation.
AB015345 mRNA. Translation: BAA34802.1.
RefSeqNP_071400.1. NM_022117.3.
UniGeneHs.136164.
Hs.592916.

3D structure databases

ProteinModelPortalQ9H2G4.
SMRQ9H2G4. Positions 210-396.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid122034. 14 interactions.
IntActQ9H2G4. 8 interactions.
MINTMINT-4719361.
STRING9606.ENSP00000364591.

PTM databases

PhosphoSiteQ9H2G4.

Polymorphism databases

DMDM74752604.

Proteomic databases

PaxDbQ9H2G4.
PRIDEQ9H2G4.

Protocols and materials databases

DNASU64061.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000375442; ENSP00000364591; ENSG00000184205.
ENST00000598656; ENSP00000469414; ENSG00000268269.
GeneID64061.
KEGGhsa:64061.
UCSCuc004drw.3. human.

Organism-specific databases

CTD64061.
GeneCardsGC0XP053111.
HGNCHGNC:24358. TSPYL2.
HPAHPA044133.
MIM300564. gene.
neXtProtNX_Q9H2G4.
PharmGKBPA134930188.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG295286.
HOGENOMHOG000154699.
HOVERGENHBG014779.
InParanoidQ9H2G4.
KOK11285.
OMADSESPDH.
PhylomeDBQ9H2G4.
TreeFamTF313386.

Enzyme and pathway databases

ReactomeREACT_17015. Metabolism of proteins.

Gene expression databases

ArrayExpressQ9H2G4.
BgeeQ9H2G4.
CleanExHS_TSPYL2.
GenevestigatorQ9H2G4.

Family and domain databases

InterProIPR002164. NAP_family.
[Graphical view]
PANTHERPTHR11875. PTHR11875. 1 hit.
PfamPF00956. NAP. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSTSPYL2. human.
GeneWikiTSPYL2.
GenomeRNAi64061.
NextBio65816.
PROQ9H2G4.
SOURCESearch...

Entry information

Entry nameTSYL2_HUMAN
AccessionPrimary (citable) accession number: Q9H2G4
Secondary accession number(s): O94799, Q96DG7, Q9BZW6
Entry history
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: March 1, 2001
Last modified: April 16, 2014
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome X

Human chromosome X: entries, gene names and cross-references to MIM