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Protein

Testis-specific Y-encoded-like protein 2

Gene

TSPYL2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Part of the CASK/TBR1/TSPYL2 transcriptional complex which modulates gene expression in response to neuronal synaptic activity, probably by facilitating nucleosome assembly. May inhibit cell proliferation by inducing p53-dependent CDKN1A expression.2 Publications

Miscellaneous

Synaptic activity down-regulates TSPYL2 protein levels by inducing rapid proteasomal degradation.By similarity
Subject to X inactivation.

GO - Molecular functioni

  • rDNA binding Source: UniProtKB

GO - Biological processi

  • cell cycle Source: UniProtKB-KW
  • IRE1-mediated unfolded protein response Source: Reactome
  • negative regulation of cell cycle Source: UniProtKB
  • negative regulation of cell growth Source: UniProtKB
  • negative regulation of DNA replication Source: UniProtKB
  • nucleosome assembly Source: InterPro
  • regulation of protein kinase activity Source: UniProtKB
  • regulation of signal transduction Source: UniProtKB
  • regulation of transcription, DNA-templated Source: UniProtKB-KW
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Molecular functionChromatin regulator
Biological processCell cycle, Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-HSA-381038 XBP1(S) activates chaperone genes
SIGNORiQ9H2G4

Names & Taxonomyi

Protein namesi
Recommended name:
Testis-specific Y-encoded-like protein 2
Short name:
TSPY-like protein 2
Alternative name(s):
Cell division autoantigen 1
Cutaneous T-cell lymphoma-associated antigen se20-4
Short name:
CTCL-associated antigen se20-4
Differentially-expressed nucleolar TGF-beta1 target protein
Nuclear protein of 79 kDa
Short name:
NP79
Gene namesi
Name:TSPYL2
Synonyms:CDA1, DENTT, TSPX
ORF Names:HRIHFB2216
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome X

Organism-specific databases

EuPathDBiHostDB:ENSG00000184205.14
HGNCiHGNC:24358 TSPYL2
MIMi300564 gene
neXtProtiNX_Q9H2G4

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi20S → A: Impairs effect on cell proliferation; when associated with A-340. 1 Publication1
Mutagenesisi340T → A: Impairs effect on cell proliferation; when associated with A-20. 1 Publication1

Organism-specific databases

DisGeNETi64061
OpenTargetsiENSG00000184205
PharmGKBiPA134930188

Polymorphism and mutation databases

BioMutaiTSPYL2
DMDMi74752604

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002891001 – 693Testis-specific Y-encoded-like protein 2Add BLAST693

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki11Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei18PhosphoserineCombined sources1
Modified residuei20Phosphoserine1 Publication1
Cross-linki163Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki165Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei340Phosphothreonine1 Publication1
Modified residuei658PhosphoserineBy similarity1
Modified residuei668PhosphoserineBy similarity1
Modified residuei671PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylation at Ser-20 and/or Thr-340 impairs function on cell proliferation.1 Publication

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiQ9H2G4
PaxDbiQ9H2G4
PeptideAtlasiQ9H2G4
PRIDEiQ9H2G4
ProteomicsDBi80546

PTM databases

iPTMnetiQ9H2G4
PhosphoSitePlusiQ9H2G4

Expressioni

Tissue specificityi

Ubiquitously expressed, with highest levels in brain, testis and heart, and lowest levels in liver and pancreas.4 Publications

Inductioni

Up-regulated in growth-arrested T-cells. Induced by TGFB1 and all-trans retinoic acid (ATRA) in lung cancer cells (at protein level).3 Publications

Gene expression databases

BgeeiENSG00000184205
CleanExiHS_TSPYL2
ExpressionAtlasiQ9H2G4 baseline and differential
GenevisibleiQ9H2G4 HS

Organism-specific databases

HPAiHPA044133

Interactioni

Subunit structurei

Interacts with histones. Interacts with CASK. Part of a complex containing CASK, TBR1 and TSPYL2 (By similarity).By similarity

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi122034, 30 interactors
IntActiQ9H2G4, 43 interactors
STRINGi9606.ENSP00000364591

Structurei

3D structure databases

ProteinModelPortaliQ9H2G4
SMRiQ9H2G4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi4 – 75Pro-richAdd BLAST72
Compositional biasi180 – 211Arg-richAdd BLAST32
Compositional biasi473 – 542Asn-richAdd BLAST70
Compositional biasi557 – 675Asp-richAdd BLAST119

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1508 Eukaryota
ENOG410XQRX LUCA
GeneTreeiENSGT00530000062882
HOGENOMiHOG000154699
HOVERGENiHBG014779
InParanoidiQ9H2G4
KOiK11285
OMAiESPDHNE
OrthoDBiEOG091G076D
PhylomeDBiQ9H2G4
TreeFamiTF313386

Family and domain databases

InterProiView protein in InterPro
IPR037231 NAP-like_sf
IPR002164 NAP_family
PANTHERiPTHR11875 PTHR11875, 1 hit
PfamiView protein in Pfam
PF00956 NAP, 1 hit
SUPFAMiSSF143113 SSF143113, 1 hit

Sequencei

Sequence statusi: Complete.

Q9H2G4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDRPDEGPPA KTRRLSSSES PQRDPPPPPP PPPLLRLPLP PPQQRPRLQE
60 70 80 90 100
ETEAAQVLAD MRGVGLGPAL PPPPPYVILE EGGIRAYFTL GAECPGWDST
110 120 130 140 150
IESGYGEAPP PTESLEALPT PEASGGSLEI DFQVVQSSSF GGEGALETCS
160 170 180 190 200
AVGWAPQRLV DPKSKEEAII IVEDEDEDER ESMRSSRRRR RRRRRKQRKV
210 220 230 240 250
KRESRERNAE RMESILQALE DIQLDLEAVN IKAGKAFLRL KRKFIQMRRP
260 270 280 290 300
FLERRDLIIQ HIPGFWVKAF LNHPRISILI NRRDEDIFRY LTNLQVQDLR
310 320 330 340 350
HISMGYKMKL YFQTNPYFTN MVIVKEFQRN RSGRLVSHST PIRWHRGQEP
360 370 380 390 400
QARRHGNQDA SHSFFSWFSN HSLPEADRIA EIIKNDLWVN PLRYYLRERG
410 420 430 440 450
SRIKRKKQEM KKRKTRGRCE VVIMEDAPDY YAVEDIFSEI SDIDETIHDI
460 470 480 490 500
KISDFMETTD YFETTDNEIT DINENICDSE NPDHNEVPNN ETTDNNESAD
510 520 530 540 550
DHETTDNNES ADDNNENPED NNKNTDDNEE NPNNNENTYG NNFFKGGFWG
560 570 580 590 600
SHGNNQDSSD SDNEADEASD DEDNDGNEGD NEGSDDDGNE GDNEGSDDDD
610 620 630 640 650
RDIEYYEKVI EDFDKDQADY EDVIEIISDE SVEEEGIEEG IQQDEDIYEE
660 670 680 690
GNYEEEGSED VWEEGEDSDD SDLEDVLQVP NGWANPGKRG KTG
Length:693
Mass (Da):79,435
Last modified:March 1, 2001 - v1
Checksum:i726F3BDC10A36165
GO

Sequence cautioni

The sequence AAG53596 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti442D → N in AAG53596 (PubMed:11318608).Curated1
Sequence conflicti566D → V in AAG53596 (PubMed:11318608).Curated1
Sequence conflicti609V → G in BAA34802 (PubMed:9853615).Curated1
Sequence conflicti615K → R in BAA34802 (PubMed:9853615).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_075536262I → M Found in patients with mild intellectual disability, unknown pathological significance. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF273046 mRNA Translation: AAG34906.1
AY040871 mRNA Translation: AAK72407.1
AL713652 mRNA Translation: CAD28461.1
BX322635 Genomic DNA No translation available.
BC001566 mRNA Translation: AAH01566.1
BC024270 mRNA Translation: AAH24270.1
AF254794 mRNA Translation: AAG53596.1 Different initiation.
AB015345 mRNA Translation: BAA34802.1
CCDSiCCDS14350.1
RefSeqiNP_071400.1, NM_022117.3
UniGeneiHs.136164
Hs.592916

Genome annotation databases

EnsembliENST00000375442; ENSP00000364591; ENSG00000184205
GeneIDi64061
KEGGihsa:64061
UCSCiuc004drw.4 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiTSYL2_HUMAN
AccessioniPrimary (citable) accession number: Q9H2G4
Secondary accession number(s): O94799, Q96DG7, Q9BZW6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: March 1, 2001
Last modified: June 20, 2018
This is version 145 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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