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Q9H2G4

- TSYL2_HUMAN

UniProt

Q9H2G4 - TSYL2_HUMAN

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Protein
Testis-specific Y-encoded-like protein 2
Gene
TSPYL2, CDA1, DENTT, TSPX, HRIHFB2216
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Part of the CASK/TBR1/TSPYL2 transcriptional complex which modulates gene expression in response to neuronal synaptic activity, probably by facilitating nucleosome assembly. May inhibit cell proliferation by inducing p53-dependent CDKN1A expression.2 Publications

GO - Molecular functioni

  1. rDNA binding Source: UniProtKB
Complete GO annotation...

GO - Biological processi

  1. activation of signaling protein activity involved in unfolded protein response Source: Reactome
  2. cell cycle Source: UniProtKB-KW
  3. cellular protein metabolic process Source: Reactome
  4. chromatin modification Source: UniProtKB-KW
  5. endoplasmic reticulum unfolded protein response Source: Reactome
  6. negative regulation of DNA replication Source: UniProtKB
  7. negative regulation of cell cycle Source: UniProtKB
  8. negative regulation of cell growth Source: UniProtKB
  9. nucleosome assembly Source: InterPro
  10. regulation of protein kinase activity Source: UniProtKB
  11. regulation of signal transduction Source: UniProtKB
  12. regulation of transcription, DNA-templated Source: UniProtKB-KW
  13. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Chromatin regulator

Keywords - Biological processi

Cell cycle, Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiREACT_18273. XBP1(S) activates chaperone genes.

Names & Taxonomyi

Protein namesi
Recommended name:
Testis-specific Y-encoded-like protein 2
Short name:
TSPY-like protein 2
Alternative name(s):
Cell division autoantigen 1
Cutaneous T-cell lymphoma-associated antigen se20-4
Short name:
CTCL-associated antigen se20-4
Differentially-expressed nucleolar TGF-beta1 target protein
Nuclear protein of 79 kDa
Short name:
NP79
Gene namesi
Name:TSPYL2
Synonyms:CDA1, DENTT, TSPX
ORF Names:HRIHFB2216
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome X

Organism-specific databases

HGNCiHGNC:24358. TSPYL2.

Subcellular locationi

Nucleus. Cytoplasm By similarity
Note: Enriched in transcriptionally active regions of chromatin in neurons By similarity.2 Publications

GO - Cellular componenti

  1. centrosome Source: HPA
  2. cytoplasm Source: HPA
  3. nucleolus Source: UniProtKB
  4. nucleoplasm Source: Reactome
  5. nucleus Source: UniProtKB
  6. plasma membrane Source: HPA
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi20 – 201S → A: Impairs effect on cell proliferation; when associated with A-340. 1 Publication
Mutagenesisi340 – 3401T → A: Impairs effect on cell proliferation; when associated with A-20. 1 Publication

Organism-specific databases

PharmGKBiPA134930188.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 693693Testis-specific Y-encoded-like protein 2
PRO_0000289100Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei18 – 181Phosphoserine1 Publication
Modified residuei20 – 201Phosphoserine1 Publication
Modified residuei340 – 3401Phosphothreonine Inferred

Post-translational modificationi

Phosphorylation at Ser-20 and/or Thr-340 impairs function on cell proliferation Inferred.

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ9H2G4.
PaxDbiQ9H2G4.
PRIDEiQ9H2G4.

PTM databases

PhosphoSiteiQ9H2G4.

Expressioni

Tissue specificityi

Ubiquitously expressed, with highest levels in brain, testis and heart, and lowest levels in liver and pancreas.4 Publications

Inductioni

Up-regulated in growth-arrested T-cells. Induced by TGFB1 and all-trans retinoic acid (ATRA) in lung cancer cells (at protein level).3 Publications

Gene expression databases

ArrayExpressiQ9H2G4.
BgeeiQ9H2G4.
CleanExiHS_TSPYL2.
GenevestigatoriQ9H2G4.

Organism-specific databases

HPAiHPA044133.

Interactioni

Subunit structurei

Interacts with histones. Interacts with CASK. Part of a complex containing CASK, TBR1 and TSPYL2 By similarity.

Protein-protein interaction databases

BioGridi122034. 13 interactions.
IntActiQ9H2G4. 8 interactions.
MINTiMINT-4719361.
STRINGi9606.ENSP00000364591.

Structurei

3D structure databases

ProteinModelPortaliQ9H2G4.
SMRiQ9H2G4. Positions 210-396.

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi4 – 7572Pro-rich
Add
BLAST
Compositional biasi180 – 21132Arg-rich
Add
BLAST
Compositional biasi473 – 54270Asn-rich
Add
BLAST
Compositional biasi557 – 675119Asp-rich
Add
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiNOG295286.
HOGENOMiHOG000154699.
HOVERGENiHBG014779.
InParanoidiQ9H2G4.
KOiK11285.
OMAiDSESPDH.
PhylomeDBiQ9H2G4.
TreeFamiTF313386.

Family and domain databases

InterProiIPR002164. NAP_family.
[Graphical view]
PANTHERiPTHR11875. PTHR11875. 1 hit.
PfamiPF00956. NAP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9H2G4-1 [UniParc]FASTAAdd to Basket

« Hide

MDRPDEGPPA KTRRLSSSES PQRDPPPPPP PPPLLRLPLP PPQQRPRLQE    50
ETEAAQVLAD MRGVGLGPAL PPPPPYVILE EGGIRAYFTL GAECPGWDST 100
IESGYGEAPP PTESLEALPT PEASGGSLEI DFQVVQSSSF GGEGALETCS 150
AVGWAPQRLV DPKSKEEAII IVEDEDEDER ESMRSSRRRR RRRRRKQRKV 200
KRESRERNAE RMESILQALE DIQLDLEAVN IKAGKAFLRL KRKFIQMRRP 250
FLERRDLIIQ HIPGFWVKAF LNHPRISILI NRRDEDIFRY LTNLQVQDLR 300
HISMGYKMKL YFQTNPYFTN MVIVKEFQRN RSGRLVSHST PIRWHRGQEP 350
QARRHGNQDA SHSFFSWFSN HSLPEADRIA EIIKNDLWVN PLRYYLRERG 400
SRIKRKKQEM KKRKTRGRCE VVIMEDAPDY YAVEDIFSEI SDIDETIHDI 450
KISDFMETTD YFETTDNEIT DINENICDSE NPDHNEVPNN ETTDNNESAD 500
DHETTDNNES ADDNNENPED NNKNTDDNEE NPNNNENTYG NNFFKGGFWG 550
SHGNNQDSSD SDNEADEASD DEDNDGNEGD NEGSDDDGNE GDNEGSDDDD 600
RDIEYYEKVI EDFDKDQADY EDVIEIISDE SVEEEGIEEG IQQDEDIYEE 650
GNYEEEGSED VWEEGEDSDD SDLEDVLQVP NGWANPGKRG KTG 693
Length:693
Mass (Da):79,435
Last modified:March 1, 2001 - v1
Checksum:i726F3BDC10A36165
GO

Sequence cautioni

The sequence AAG53596.1 differs from that shown. Reason: Erroneous initiation.

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti442 – 4421D → N in AAG53596. 1 Publication
Sequence conflicti566 – 5661D → V in AAG53596. 1 Publication
Sequence conflicti609 – 6091V → G in BAA34802. 1 Publication
Sequence conflicti615 – 6151K → R in BAA34802. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF273046 mRNA. Translation: AAG34906.1.
AY040871 mRNA. Translation: AAK72407.1.
AL713652 mRNA. Translation: CAD28461.1.
BX322635 Genomic DNA. Translation: CAI42531.1.
BC001566 mRNA. Translation: AAH01566.1.
BC024270 mRNA. Translation: AAH24270.1.
AF254794 mRNA. Translation: AAG53596.1. Different initiation.
AB015345 mRNA. Translation: BAA34802.1.
CCDSiCCDS14350.1.
RefSeqiNP_071400.1. NM_022117.3.
UniGeneiHs.136164.
Hs.592916.

Genome annotation databases

EnsembliENST00000375442; ENSP00000364591; ENSG00000184205.
ENST00000598656; ENSP00000469414; ENSG00000268269.
GeneIDi64061.
KEGGihsa:64061.
UCSCiuc004drw.3. human.

Polymorphism databases

DMDMi74752604.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF273046 mRNA. Translation: AAG34906.1 .
AY040871 mRNA. Translation: AAK72407.1 .
AL713652 mRNA. Translation: CAD28461.1 .
BX322635 Genomic DNA. Translation: CAI42531.1 .
BC001566 mRNA. Translation: AAH01566.1 .
BC024270 mRNA. Translation: AAH24270.1 .
AF254794 mRNA. Translation: AAG53596.1 . Different initiation.
AB015345 mRNA. Translation: BAA34802.1 .
CCDSi CCDS14350.1.
RefSeqi NP_071400.1. NM_022117.3.
UniGenei Hs.136164.
Hs.592916.

3D structure databases

ProteinModelPortali Q9H2G4.
SMRi Q9H2G4. Positions 210-396.
ModBasei Search...

Protein-protein interaction databases

BioGridi 122034. 13 interactions.
IntActi Q9H2G4. 8 interactions.
MINTi MINT-4719361.
STRINGi 9606.ENSP00000364591.

PTM databases

PhosphoSitei Q9H2G4.

Polymorphism databases

DMDMi 74752604.

Proteomic databases

MaxQBi Q9H2G4.
PaxDbi Q9H2G4.
PRIDEi Q9H2G4.

Protocols and materials databases

DNASUi 64061.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000375442 ; ENSP00000364591 ; ENSG00000184205 .
ENST00000598656 ; ENSP00000469414 ; ENSG00000268269 .
GeneIDi 64061.
KEGGi hsa:64061.
UCSCi uc004drw.3. human.

Organism-specific databases

CTDi 64061.
GeneCardsi GC0XP053111.
HGNCi HGNC:24358. TSPYL2.
HPAi HPA044133.
MIMi 300564. gene.
neXtProti NX_Q9H2G4.
PharmGKBi PA134930188.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG295286.
HOGENOMi HOG000154699.
HOVERGENi HBG014779.
InParanoidi Q9H2G4.
KOi K11285.
OMAi DSESPDH.
PhylomeDBi Q9H2G4.
TreeFami TF313386.

Enzyme and pathway databases

Reactomei REACT_18273. XBP1(S) activates chaperone genes.

Miscellaneous databases

ChiTaRSi TSPYL2. human.
GeneWikii TSPYL2.
GenomeRNAii 64061.
NextBioi 65816.
PROi Q9H2G4.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q9H2G4.
Bgeei Q9H2G4.
CleanExi HS_TSPYL2.
Genevestigatori Q9H2G4.

Family and domain databases

InterProi IPR002164. NAP_family.
[Graphical view ]
PANTHERi PTHR11875. PTHR11875. 1 hit.
Pfami PF00956. NAP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "SET-related cell division autoantigen-1 (CDA1) arrests cell growth."
    Chai Z., Sarcevic B., Mawson A., Toh B.-H.
    J. Biol. Chem. 276:33665-33674(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, PHOSPHORYLATION AT SER-20 AND THR-340, MUTAGENESIS OF SER-20 AND THR-340, FUNCTION.
    Tissue: Testis.
  2. "Serological detection of cutaneous T-cell lymphoma-associated antigens."
    Eichmueller S., Usener D., Dummer R., Stein A., Thiel D., Schadendorf D.
    Proc. Natl. Acad. Sci. U.S.A. 98:629-634(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    Tissue: Testis.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Amygdala.
  4. "The DNA sequence of the human X chromosome."
    Ross M.T., Grafham D.V., Coffey A.J., Scherer S., McLay K., Muzny D., Platzer M., Howell G.R., Burrows C., Bird C.P., Frankish A., Lovell F.L., Howe K.L., Ashurst J.L., Fulton R.S., Sudbrak R., Wen G., Jones M.C.
    , Hurles M.E., Andrews T.D., Scott C.E., Searle S., Ramser J., Whittaker A., Deadman R., Carter N.P., Hunt S.E., Chen R., Cree A., Gunaratne P., Havlak P., Hodgson A., Metzker M.L., Richards S., Scott G., Steffen D., Sodergren E., Wheeler D.A., Worley K.C., Ainscough R., Ambrose K.D., Ansari-Lari M.A., Aradhya S., Ashwell R.I., Babbage A.K., Bagguley C.L., Ballabio A., Banerjee R., Barker G.E., Barlow K.F., Barrett I.P., Bates K.N., Beare D.M., Beasley H., Beasley O., Beck A., Bethel G., Blechschmidt K., Brady N., Bray-Allen S., Bridgeman A.M., Brown A.J., Brown M.J., Bonnin D., Bruford E.A., Buhay C., Burch P., Burford D., Burgess J., Burrill W., Burton J., Bye J.M., Carder C., Carrel L., Chako J., Chapman J.C., Chavez D., Chen E., Chen G., Chen Y., Chen Z., Chinault C., Ciccodicola A., Clark S.Y., Clarke G., Clee C.M., Clegg S., Clerc-Blankenburg K., Clifford K., Cobley V., Cole C.G., Conquer J.S., Corby N., Connor R.E., David R., Davies J., Davis C., Davis J., Delgado O., Deshazo D., Dhami P., Ding Y., Dinh H., Dodsworth S., Draper H., Dugan-Rocha S., Dunham A., Dunn M., Durbin K.J., Dutta I., Eades T., Ellwood M., Emery-Cohen A., Errington H., Evans K.L., Faulkner L., Francis F., Frankland J., Fraser A.E., Galgoczy P., Gilbert J., Gill R., Gloeckner G., Gregory S.G., Gribble S., Griffiths C., Grocock R., Gu Y., Gwilliam R., Hamilton C., Hart E.A., Hawes A., Heath P.D., Heitmann K., Hennig S., Hernandez J., Hinzmann B., Ho S., Hoffs M., Howden P.J., Huckle E.J., Hume J., Hunt P.J., Hunt A.R., Isherwood J., Jacob L., Johnson D., Jones S., de Jong P.J., Joseph S.S., Keenan S., Kelly S., Kershaw J.K., Khan Z., Kioschis P., Klages S., Knights A.J., Kosiura A., Kovar-Smith C., Laird G.K., Langford C., Lawlor S., Leversha M., Lewis L., Liu W., Lloyd C., Lloyd D.M., Loulseged H., Loveland J.E., Lovell J.D., Lozado R., Lu J., Lyne R., Ma J., Maheshwari M., Matthews L.H., McDowall J., McLaren S., McMurray A., Meidl P., Meitinger T., Milne S., Miner G., Mistry S.L., Morgan M., Morris S., Mueller I., Mullikin J.C., Nguyen N., Nordsiek G., Nyakatura G., O'dell C.N., Okwuonu G., Palmer S., Pandian R., Parker D., Parrish J., Pasternak S., Patel D., Pearce A.V., Pearson D.M., Pelan S.E., Perez L., Porter K.M., Ramsey Y., Reichwald K., Rhodes S., Ridler K.A., Schlessinger D., Schueler M.G., Sehra H.K., Shaw-Smith C., Shen H., Sheridan E.M., Shownkeen R., Skuce C.D., Smith M.L., Sotheran E.C., Steingruber H.E., Steward C.A., Storey R., Swann R.M., Swarbreck D., Tabor P.E., Taudien S., Taylor T., Teague B., Thomas K., Thorpe A., Timms K., Tracey A., Trevanion S., Tromans A.C., d'Urso M., Verduzco D., Villasana D., Waldron L., Wall M., Wang Q., Warren J., Warry G.L., Wei X., West A., Whitehead S.L., Whiteley M.N., Wilkinson J.E., Willey D.L., Williams G., Williams L., Williamson A., Williamson H., Wilming L., Woodmansey R.L., Wray P.W., Yen J., Zhang J., Zhou J., Zoghbi H., Zorilla S., Buck D., Reinhardt R., Poustka A., Rosenthal A., Lehrach H., Meindl A., Minx P.J., Hillier L.W., Willard H.F., Wilson R.K., Waterston R.H., Rice C.M., Vaudin M., Coulson A., Nelson D.L., Weinstock G., Sulston J.E., Durbin R.M., Hubbard T., Gibbs R.A., Beck S., Rogers J., Bentley D.R.
    Nature 434:325-337(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Lung and Placenta.
  6. "Identification of Differentially expressed nucleolar TGF-beta1 target (DENTT) in human lung cancer cells that is a new member of the TSPY/SET/NAP-1 superfamily."
    Ozbun L.L., You L., Kiang S., Angdisen J., Martinez A., Jakowlew S.B.
    Genomics 73:179-193(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 42-693, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, INDUCTION BY TGFB1.
  7. "Selection system for genes encoding nuclear-targeted proteins."
    Ueki N., Oda T., Kondo M., Yano K., Noguchi T., Muramatsu M.-A.
    Nat. Biotechnol. 16:1338-1342(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 208-693.
    Tissue: Fetal brain.
  8. "Isolation of differentially expressed genes in human heart tissues."
    Sun G., Yuen Chan S., Yuan Y., Wang Chan K., Qiu G., Sun K., Ping Leung M.
    Biochim. Biophys. Acta 1588:241-246(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION, TISSUE SPECIFICITY.
  9. "TSPY, the candidate gonadoblastoma gene on the human Y chromosome, has a widely expressed homologue on the X -- implications for Y chromosome evolution."
    Delbridge M.L., Longepied G., Depetris D., Mattei M.-G., Disteche C.M., Marshall Graves J.A., Mitchell M.J.
    Chromosome Res. 12:345-356(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION, TISSUE SPECIFICITY.
  10. "A constitutively active arylhydrocarbon receptor induces growth inhibition of jurkat T cells through changes in the expression of genes related to apoptosis and cell cycle arrest."
    Ito T., Tsukumo S., Suzuki N., Motohashi H., Yamamoto M., Fujii-Kuriyama Y., Mimura J., Lin T.-M., Peterson R.E., Tohyama C., Nohara K.
    J. Biol. Chem. 279:25204-25210(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  11. "Differentially expressed nucleolar TGF-beta1 target (DENTT) shows tissue-specific nuclear and cytoplasmic localization and increases TGF-beta1-responsive transcription in primates."
    Ozbun L.L., Martinez A., Jakowlew S.B.
    Biochim. Biophys. Acta 1728:163-180(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  12. "Antiproliferative autoantigen CDA1 transcriptionally up-regulates p21(Waf1/Cip1) by activating p53 and MEK/ERK1/2 MAPK pathways."
    Tu Y., Wu W., Wu T., Cao Z., Wilkins R., Toh B.-H., Cooper M.E., Chai Z.
    J. Biol. Chem. 282:11722-11731(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  13. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-18, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  14. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
    Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
    Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.

Entry informationi

Entry nameiTSYL2_HUMAN
AccessioniPrimary (citable) accession number: Q9H2G4
Secondary accession number(s): O94799, Q96DG7, Q9BZW6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: March 1, 2001
Last modified: September 3, 2014
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Miscellaneous

Synaptic activity down-regulates TSPYL2 protein levels by inducing rapid proteasomal degradation By similarity.
Subject to X inactivation.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome X
    Human chromosome X: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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