Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Testis-specific Y-encoded-like protein 2

Gene

TSPYL2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Part of the CASK/TBR1/TSPYL2 transcriptional complex which modulates gene expression in response to neuronal synaptic activity, probably by facilitating nucleosome assembly. May inhibit cell proliferation by inducing p53-dependent CDKN1A expression.2 Publications

GO - Molecular functioni

  • rDNA binding Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Chromatin regulator

Keywords - Biological processi

Cell cycle, Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiREACT_18273. XBP1(S) activates chaperone genes.

Names & Taxonomyi

Protein namesi
Recommended name:
Testis-specific Y-encoded-like protein 2
Short name:
TSPY-like protein 2
Alternative name(s):
Cell division autoantigen 1
Cutaneous T-cell lymphoma-associated antigen se20-4
Short name:
CTCL-associated antigen se20-4
Differentially-expressed nucleolar TGF-beta1 target protein
Nuclear protein of 79 kDa
Short name:
NP79
Gene namesi
Name:TSPYL2
Synonyms:CDA1, DENTT, TSPX
ORF Names:HRIHFB2216
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome X

Organism-specific databases

HGNCiHGNC:24358. TSPYL2.

Subcellular locationi

GO - Cellular componenti

  • centrosome Source: HPA
  • cytoplasm Source: HPA
  • nucleolus Source: UniProtKB
  • nucleoplasm Source: HPA
  • nucleus Source: UniProtKB
  • plasma membrane Source: HPA
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi20 – 201S → A: Impairs effect on cell proliferation; when associated with A-340. 1 Publication
Mutagenesisi340 – 3401T → A: Impairs effect on cell proliferation; when associated with A-20. 1 Publication

Organism-specific databases

PharmGKBiPA134930188.

Polymorphism and mutation databases

BioMutaiTSPYL2.
DMDMi74752604.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 693693Testis-specific Y-encoded-like protein 2PRO_0000289100Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei18 – 181Phosphoserine1 Publication
Modified residuei20 – 201Phosphoserine1 Publication
Modified residuei340 – 3401Phosphothreonine1 Publication

Post-translational modificationi

Phosphorylation at Ser-20 and/or Thr-340 impairs function on cell proliferation.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ9H2G4.
PaxDbiQ9H2G4.
PRIDEiQ9H2G4.

PTM databases

PhosphoSiteiQ9H2G4.

Expressioni

Tissue specificityi

Ubiquitously expressed, with highest levels in brain, testis and heart, and lowest levels in liver and pancreas.4 Publications

Inductioni

Up-regulated in growth-arrested T-cells. Induced by TGFB1 and all-trans retinoic acid (ATRA) in lung cancer cells (at protein level).3 Publications

Gene expression databases

BgeeiQ9H2G4.
CleanExiHS_TSPYL2.
ExpressionAtlasiQ9H2G4. baseline and differential.
GenevisibleiQ9H2G4. HS.

Organism-specific databases

HPAiHPA044133.

Interactioni

Subunit structurei

Interacts with histones. Interacts with CASK. Part of a complex containing CASK, TBR1 and TSPYL2 (By similarity).By similarity

Protein-protein interaction databases

BioGridi122034. 19 interactions.
IntActiQ9H2G4. 8 interactions.
MINTiMINT-4719361.

Structurei

3D structure databases

ProteinModelPortaliQ9H2G4.
SMRiQ9H2G4. Positions 210-396.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi4 – 7572Pro-richAdd
BLAST
Compositional biasi180 – 21132Arg-richAdd
BLAST
Compositional biasi473 – 54270Asn-richAdd
BLAST
Compositional biasi557 – 675119Asp-richAdd
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiNOG295286.
GeneTreeiENSGT00530000062882.
HOGENOMiHOG000154699.
HOVERGENiHBG014779.
InParanoidiQ9H2G4.
KOiK11285.
OMAiDSESPDH.
PhylomeDBiQ9H2G4.
TreeFamiTF313386.

Family and domain databases

InterProiIPR002164. NAP_family.
[Graphical view]
PANTHERiPTHR11875. PTHR11875. 1 hit.
PfamiPF00956. NAP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9H2G4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDRPDEGPPA KTRRLSSSES PQRDPPPPPP PPPLLRLPLP PPQQRPRLQE
60 70 80 90 100
ETEAAQVLAD MRGVGLGPAL PPPPPYVILE EGGIRAYFTL GAECPGWDST
110 120 130 140 150
IESGYGEAPP PTESLEALPT PEASGGSLEI DFQVVQSSSF GGEGALETCS
160 170 180 190 200
AVGWAPQRLV DPKSKEEAII IVEDEDEDER ESMRSSRRRR RRRRRKQRKV
210 220 230 240 250
KRESRERNAE RMESILQALE DIQLDLEAVN IKAGKAFLRL KRKFIQMRRP
260 270 280 290 300
FLERRDLIIQ HIPGFWVKAF LNHPRISILI NRRDEDIFRY LTNLQVQDLR
310 320 330 340 350
HISMGYKMKL YFQTNPYFTN MVIVKEFQRN RSGRLVSHST PIRWHRGQEP
360 370 380 390 400
QARRHGNQDA SHSFFSWFSN HSLPEADRIA EIIKNDLWVN PLRYYLRERG
410 420 430 440 450
SRIKRKKQEM KKRKTRGRCE VVIMEDAPDY YAVEDIFSEI SDIDETIHDI
460 470 480 490 500
KISDFMETTD YFETTDNEIT DINENICDSE NPDHNEVPNN ETTDNNESAD
510 520 530 540 550
DHETTDNNES ADDNNENPED NNKNTDDNEE NPNNNENTYG NNFFKGGFWG
560 570 580 590 600
SHGNNQDSSD SDNEADEASD DEDNDGNEGD NEGSDDDGNE GDNEGSDDDD
610 620 630 640 650
RDIEYYEKVI EDFDKDQADY EDVIEIISDE SVEEEGIEEG IQQDEDIYEE
660 670 680 690
GNYEEEGSED VWEEGEDSDD SDLEDVLQVP NGWANPGKRG KTG
Length:693
Mass (Da):79,435
Last modified:March 1, 2001 - v1
Checksum:i726F3BDC10A36165
GO

Sequence cautioni

The sequence AAG53596.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti442 – 4421D → N in AAG53596 (PubMed:11318608).Curated
Sequence conflicti566 – 5661D → V in AAG53596 (PubMed:11318608).Curated
Sequence conflicti609 – 6091V → G in BAA34802 (PubMed:9853615).Curated
Sequence conflicti615 – 6151K → R in BAA34802 (PubMed:9853615).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF273046 mRNA. Translation: AAG34906.1.
AY040871 mRNA. Translation: AAK72407.1.
AL713652 mRNA. Translation: CAD28461.1.
BX322635 Genomic DNA. Translation: CAI42531.1.
BC001566 mRNA. Translation: AAH01566.1.
BC024270 mRNA. Translation: AAH24270.1.
AF254794 mRNA. Translation: AAG53596.1. Different initiation.
AB015345 mRNA. Translation: BAA34802.1.
CCDSiCCDS14350.1.
RefSeqiNP_071400.1. NM_022117.3.
UniGeneiHs.136164.
Hs.592916.

Genome annotation databases

EnsembliENST00000375442; ENSP00000364591; ENSG00000184205.
GeneIDi64061.
KEGGihsa:64061.
UCSCiuc004drw.3. human.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF273046 mRNA. Translation: AAG34906.1.
AY040871 mRNA. Translation: AAK72407.1.
AL713652 mRNA. Translation: CAD28461.1.
BX322635 Genomic DNA. Translation: CAI42531.1.
BC001566 mRNA. Translation: AAH01566.1.
BC024270 mRNA. Translation: AAH24270.1.
AF254794 mRNA. Translation: AAG53596.1. Different initiation.
AB015345 mRNA. Translation: BAA34802.1.
CCDSiCCDS14350.1.
RefSeqiNP_071400.1. NM_022117.3.
UniGeneiHs.136164.
Hs.592916.

3D structure databases

ProteinModelPortaliQ9H2G4.
SMRiQ9H2G4. Positions 210-396.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi122034. 19 interactions.
IntActiQ9H2G4. 8 interactions.
MINTiMINT-4719361.

PTM databases

PhosphoSiteiQ9H2G4.

Polymorphism and mutation databases

BioMutaiTSPYL2.
DMDMi74752604.

Proteomic databases

MaxQBiQ9H2G4.
PaxDbiQ9H2G4.
PRIDEiQ9H2G4.

Protocols and materials databases

DNASUi64061.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000375442; ENSP00000364591; ENSG00000184205.
GeneIDi64061.
KEGGihsa:64061.
UCSCiuc004drw.3. human.

Organism-specific databases

CTDi64061.
GeneCardsiGC0XP053111.
HGNCiHGNC:24358. TSPYL2.
HPAiHPA044133.
MIMi300564. gene.
neXtProtiNX_Q9H2G4.
PharmGKBiPA134930188.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG295286.
GeneTreeiENSGT00530000062882.
HOGENOMiHOG000154699.
HOVERGENiHBG014779.
InParanoidiQ9H2G4.
KOiK11285.
OMAiDSESPDH.
PhylomeDBiQ9H2G4.
TreeFamiTF313386.

Enzyme and pathway databases

ReactomeiREACT_18273. XBP1(S) activates chaperone genes.

Miscellaneous databases

ChiTaRSiTSPYL2. human.
GeneWikiiTSPYL2.
GenomeRNAii64061.
NextBioi65816.
PROiQ9H2G4.
SOURCEiSearch...

Gene expression databases

BgeeiQ9H2G4.
CleanExiHS_TSPYL2.
ExpressionAtlasiQ9H2G4. baseline and differential.
GenevisibleiQ9H2G4. HS.

Family and domain databases

InterProiIPR002164. NAP_family.
[Graphical view]
PANTHERiPTHR11875. PTHR11875. 1 hit.
PfamiPF00956. NAP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "SET-related cell division autoantigen-1 (CDA1) arrests cell growth."
    Chai Z., Sarcevic B., Mawson A., Toh B.-H.
    J. Biol. Chem. 276:33665-33674(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, PHOSPHORYLATION AT SER-20 AND THR-340, MUTAGENESIS OF SER-20 AND THR-340, FUNCTION.
    Tissue: Testis.
  2. "Serological detection of cutaneous T-cell lymphoma-associated antigens."
    Eichmueller S., Usener D., Dummer R., Stein A., Thiel D., Schadendorf D.
    Proc. Natl. Acad. Sci. U.S.A. 98:629-634(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    Tissue: Testis.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Amygdala.
  4. "The DNA sequence of the human X chromosome."
    Ross M.T., Grafham D.V., Coffey A.J., Scherer S., McLay K., Muzny D., Platzer M., Howell G.R., Burrows C., Bird C.P., Frankish A., Lovell F.L., Howe K.L., Ashurst J.L., Fulton R.S., Sudbrak R., Wen G., Jones M.C.
    , Hurles M.E., Andrews T.D., Scott C.E., Searle S., Ramser J., Whittaker A., Deadman R., Carter N.P., Hunt S.E., Chen R., Cree A., Gunaratne P., Havlak P., Hodgson A., Metzker M.L., Richards S., Scott G., Steffen D., Sodergren E., Wheeler D.A., Worley K.C., Ainscough R., Ambrose K.D., Ansari-Lari M.A., Aradhya S., Ashwell R.I., Babbage A.K., Bagguley C.L., Ballabio A., Banerjee R., Barker G.E., Barlow K.F., Barrett I.P., Bates K.N., Beare D.M., Beasley H., Beasley O., Beck A., Bethel G., Blechschmidt K., Brady N., Bray-Allen S., Bridgeman A.M., Brown A.J., Brown M.J., Bonnin D., Bruford E.A., Buhay C., Burch P., Burford D., Burgess J., Burrill W., Burton J., Bye J.M., Carder C., Carrel L., Chako J., Chapman J.C., Chavez D., Chen E., Chen G., Chen Y., Chen Z., Chinault C., Ciccodicola A., Clark S.Y., Clarke G., Clee C.M., Clegg S., Clerc-Blankenburg K., Clifford K., Cobley V., Cole C.G., Conquer J.S., Corby N., Connor R.E., David R., Davies J., Davis C., Davis J., Delgado O., Deshazo D., Dhami P., Ding Y., Dinh H., Dodsworth S., Draper H., Dugan-Rocha S., Dunham A., Dunn M., Durbin K.J., Dutta I., Eades T., Ellwood M., Emery-Cohen A., Errington H., Evans K.L., Faulkner L., Francis F., Frankland J., Fraser A.E., Galgoczy P., Gilbert J., Gill R., Gloeckner G., Gregory S.G., Gribble S., Griffiths C., Grocock R., Gu Y., Gwilliam R., Hamilton C., Hart E.A., Hawes A., Heath P.D., Heitmann K., Hennig S., Hernandez J., Hinzmann B., Ho S., Hoffs M., Howden P.J., Huckle E.J., Hume J., Hunt P.J., Hunt A.R., Isherwood J., Jacob L., Johnson D., Jones S., de Jong P.J., Joseph S.S., Keenan S., Kelly S., Kershaw J.K., Khan Z., Kioschis P., Klages S., Knights A.J., Kosiura A., Kovar-Smith C., Laird G.K., Langford C., Lawlor S., Leversha M., Lewis L., Liu W., Lloyd C., Lloyd D.M., Loulseged H., Loveland J.E., Lovell J.D., Lozado R., Lu J., Lyne R., Ma J., Maheshwari M., Matthews L.H., McDowall J., McLaren S., McMurray A., Meidl P., Meitinger T., Milne S., Miner G., Mistry S.L., Morgan M., Morris S., Mueller I., Mullikin J.C., Nguyen N., Nordsiek G., Nyakatura G., O'dell C.N., Okwuonu G., Palmer S., Pandian R., Parker D., Parrish J., Pasternak S., Patel D., Pearce A.V., Pearson D.M., Pelan S.E., Perez L., Porter K.M., Ramsey Y., Reichwald K., Rhodes S., Ridler K.A., Schlessinger D., Schueler M.G., Sehra H.K., Shaw-Smith C., Shen H., Sheridan E.M., Shownkeen R., Skuce C.D., Smith M.L., Sotheran E.C., Steingruber H.E., Steward C.A., Storey R., Swann R.M., Swarbreck D., Tabor P.E., Taudien S., Taylor T., Teague B., Thomas K., Thorpe A., Timms K., Tracey A., Trevanion S., Tromans A.C., d'Urso M., Verduzco D., Villasana D., Waldron L., Wall M., Wang Q., Warren J., Warry G.L., Wei X., West A., Whitehead S.L., Whiteley M.N., Wilkinson J.E., Willey D.L., Williams G., Williams L., Williamson A., Williamson H., Wilming L., Woodmansey R.L., Wray P.W., Yen J., Zhang J., Zhou J., Zoghbi H., Zorilla S., Buck D., Reinhardt R., Poustka A., Rosenthal A., Lehrach H., Meindl A., Minx P.J., Hillier L.W., Willard H.F., Wilson R.K., Waterston R.H., Rice C.M., Vaudin M., Coulson A., Nelson D.L., Weinstock G., Sulston J.E., Durbin R.M., Hubbard T., Gibbs R.A., Beck S., Rogers J., Bentley D.R.
    Nature 434:325-337(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Lung and Placenta.
  6. "Identification of Differentially expressed nucleolar TGF-beta1 target (DENTT) in human lung cancer cells that is a new member of the TSPY/SET/NAP-1 superfamily."
    Ozbun L.L., You L., Kiang S., Angdisen J., Martinez A., Jakowlew S.B.
    Genomics 73:179-193(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 42-693, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, INDUCTION BY TGFB1.
  7. "Selection system for genes encoding nuclear-targeted proteins."
    Ueki N., Oda T., Kondo M., Yano K., Noguchi T., Muramatsu M.-A.
    Nat. Biotechnol. 16:1338-1342(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 208-693.
    Tissue: Fetal brain.
  8. "Isolation of differentially expressed genes in human heart tissues."
    Sun G., Yuen Chan S., Yuan Y., Wang Chan K., Qiu G., Sun K., Ping Leung M.
    Biochim. Biophys. Acta 1588:241-246(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION, TISSUE SPECIFICITY.
  9. "TSPY, the candidate gonadoblastoma gene on the human Y chromosome, has a widely expressed homologue on the X -- implications for Y chromosome evolution."
    Delbridge M.L., Longepied G., Depetris D., Mattei M.-G., Disteche C.M., Marshall Graves J.A., Mitchell M.J.
    Chromosome Res. 12:345-356(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION, TISSUE SPECIFICITY.
  10. "A constitutively active arylhydrocarbon receptor induces growth inhibition of jurkat T cells through changes in the expression of genes related to apoptosis and cell cycle arrest."
    Ito T., Tsukumo S., Suzuki N., Motohashi H., Yamamoto M., Fujii-Kuriyama Y., Mimura J., Lin T.-M., Peterson R.E., Tohyama C., Nohara K.
    J. Biol. Chem. 279:25204-25210(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  11. "Differentially expressed nucleolar TGF-beta1 target (DENTT) shows tissue-specific nuclear and cytoplasmic localization and increases TGF-beta1-responsive transcription in primates."
    Ozbun L.L., Martinez A., Jakowlew S.B.
    Biochim. Biophys. Acta 1728:163-180(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  12. "Antiproliferative autoantigen CDA1 transcriptionally up-regulates p21(Waf1/Cip1) by activating p53 and MEK/ERK1/2 MAPK pathways."
    Tu Y., Wu W., Wu T., Cao Z., Wilkins R., Toh B.-H., Cooper M.E., Chai Z.
    J. Biol. Chem. 282:11722-11731(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  13. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-18, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  14. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
    Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
    Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.

Entry informationi

Entry nameiTSYL2_HUMAN
AccessioniPrimary (citable) accession number: Q9H2G4
Secondary accession number(s): O94799, Q96DG7, Q9BZW6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: March 1, 2001
Last modified: June 24, 2015
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Miscellaneous

Synaptic activity down-regulates TSPYL2 protein levels by inducing rapid proteasomal degradation.By similarity
Subject to X inactivation.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome X
    Human chromosome X: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.