Q9H2D6 (TARA_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
TRIO and F-actin-binding protein

Alternative name(s):
Protein Tara
Trio-associated repeat on actin

Gene names

Name:	TRIOBP
Synonyms:	KIAA1662, TARA
ORF Names:	HRIHFB2122

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	2365 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May regulate actin cytoskeletal organization, cell spreading and cell contraction by directly binding and stabilizing filamentous F-actin. The localized formation of TARA and TRIO complexes coordinates the amount of F-actin present in stress fibers. May also serve as a linker protein to recruit proteins required for F-actin formation and turnover. Ref.9
Subunit structure	Binds to TRIO and F-actin. May also interact with myosin II. Interacts with HECTD3. Ref.9
Subcellular location	Nucleus. Cytoplasm › cytoskeleton. Note: Localized to F-actin in a periodic pattern. Ref.6
Tissue specificity	Widely expressed. Highly expressed in heart and placenta. Isoform 3 is expressed in fetal brain, retina and cochlea but is not detectable in the other tissues. Ref.3
Domain	Contains at least 2 actin-binding sites per coiled-coil dimer.
Post-translational modification	Ubiquitinated by HECTD3, leading to its degradation by the proteasome. Ref.9
Involvement in disease	Deafness, autosomal recessive, 28 (DFNB28) [MIM:609823]: A form of non-syndromic sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information. Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.2 Ref.3
Miscellaneous	Has been identified in Ref.6 by a selection system for genes encoding nuclear-targeted protein.
Sequence similarities	Contains 1 PH domain.
Sequence caution	The sequence BAA34800.1 differs from that shown. Reason: Frameshift at position 478.

Ontologies

Keywords
Cellular component	Cytoplasm Cytoskeleton Nucleus
Coding sequence diversity	Alternative splicing Polymorphism
Disease	Deafness Disease mutation Non-syndromic deafness
Domain	Coiled coil
Ligand	Actin-binding
PTM	Phosphoprotein Ubl conjugation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	actin modification Non-traceable author statement Ref.1. Source: UniProtKB barbed-end actin filament capping Non-traceable author statement Ref.1. Source: UniProtKB positive regulation of substrate adhesion-dependent cell spreading Inferred from direct assay Ref.1. Source: MGI
Cellular_component	actin cytoskeleton Non-traceable author statement Ref.1. Source: UniProtKB cytoplasm Inferred from direct assay. Source: HPA nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	GTP-Rho binding Non-traceable author statement Ref.1. Source: UniProtKB actin filament binding Inferred from direct assay Ref.1. Source: MGI myosin II binding Non-traceable author statement Ref.1. Source: UniProtKB phospholipid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 5 isoforms produced by alternative splicing. [Align] [Select]

Isoform 2 (identifier: Q9H2D6-1)

Also known as: TRIOBP-6;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 3 (identifier: Q9H2D6-2)

Also known as: Long isoform;

The sequence of this isoform differs from the canonical sequence as follows:
     1317-1355: Missing.
     1729-1774: Missing.
     1794-1806: Missing.

Isoform 4 (identifier: Q9H2D6-3)

Also known as: TRIOBP-3;

The sequence of this isoform differs from the canonical sequence as follows:
     1-172: Missing.

Isoform 5 (identifier: Q9H2D6-4)

Also known as: TRIOBP-4;

The sequence of this isoform differs from the canonical sequence as follows:
     1-172: Missing.
     1317-2365: Missing.

Isoform 1 (identifier: Q9H2D6-5)

Also known as: TRIOBP-1;

The sequence of this isoform differs from the canonical sequence as follows:
     1-1772: Missing.
     1773-1774: RR → MT

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 2365	2365	TRIO and F-actin-binding protein		PRO_0000072433
Regions
	Domain	1778 – 1887	110	PH
	Coiled coil	2062 – 2247	186	Potential
	Coiled coil	2281 – 2361	81	Potential
Amino acid modifications
	Modified residue	1949	1	Phosphoserine Ref.10 Ref.12
	Modified residue	1955	1	Phosphoserine Ref.10 Ref.11 Ref.12
Natural variations
	Alternative sequence	1 – 1772	1772	Missing in isoform 1.		VSP_017711
	Alternative sequence	1 – 172	172	Missing in isoform 4 and isoform 5.		VSP_017712
	Alternative sequence	1317 – 2365	1049	Missing in isoform 5.		VSP_017713
	Alternative sequence	1317 – 1355	39	Missing in isoform 3.		VSP_017714
	Alternative sequence	1729 – 1774	46	Missing in isoform 3.		VSP_017715
	Alternative sequence	1773 – 1774	2	RR → MT in isoform 1.		VSP_017716
	Alternative sequence	1794 – 1806	13	Missing in isoform 3.		VSP_017717
	Natural variant	217	1	S → N. Corresponds to variant rs12628603 [ dbSNP | Ensembl ].		VAR_059725
	Natural variant	493	1	S → N. Corresponds to variant rs4821700 [ dbSNP | Ensembl ].		VAR_059726
	Natural variant	817	1	T → S. Corresponds to variant rs41302575 [ dbSNP | Ensembl ].		VAR_061708
	Natural variant	863	1	N → K. Corresponds to variant rs9610841 [ dbSNP | Ensembl ].		VAR_051412
	Natural variant	1019	1	G → R in DFNB28. Ref.3		VAR_025719
	Natural variant	1187	1	F → L. Corresponds to variant rs5756795 [ dbSNP | Ensembl ].		VAR_059727
	Natural variant	1300	1	H → R. Corresponds to variant rs739138 [ dbSNP | Ensembl ].		VAR_059728
	Natural variant	1372	1	E → D. Corresponds to variant rs8140207 [ dbSNP | Ensembl ].		VAR_051413
	Natural variant	1377	1	W → R. Ref.3 Ref.7 Corresponds to variant rs8140958 [ dbSNP | Ensembl ].		VAR_051414
Experimental info
	Sequence conflict	713 – 719	7	RTTQQEN → KSTFGCL in BAB33332. Ref.7
	Sequence conflict	1421	1	R → S in ABB77204. Ref.3
	Sequence conflict	1689	1	S → G in ABB77204. Ref.3
	Sequence conflict	1896	1	Missing in ABB77204. Ref.3
	Sequence conflict	1904	1	N → D in ABB77204. Ref.3
	Sequence conflict	2114	1	S → T in AAG44841. Ref.1
	Sequence conflict	2141	1	Q → L in AAH04303. Ref.5
	Sequence conflict	2274	1	S → T in AAG44841. Ref.1
	Sequence conflict	2283	1	E → EHLYPQ in AAH04303. Ref.5
	Sequence conflict	2359	1	M → I in AAG44841. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 2 (TRIOBP-6) [UniParc]. Last modified March 21, 2006. Version 3. Checksum: B2CF9AD57E930283	FASTA	2,365	261,376
	10 20 30 40 50 60 MEEVPGDALC EHFEANILTQ NRCQNCFHPE EAHGARYQEL RSPSGAEVPY CDLPRCPPAP 70 80 90 100 110 120 EDPLSASTSG CQSVVDPGLR PGPKRGPSPS AGLPEEGPTA APRSRSRELE AVPYLEGLTT 130 140 150 160 170 180 SLCGSCNEDP GSDPTSSPDS ATPDDTSNSS SVDWDTVERQ EEEAPSWDEL AVMIPRRPRE 190 200 210 220 230 240 GPRADSSQRA PSLLTRSPVG GDAAGQKKED TGGGGRSAGQ HWARLRGESG LSLERHRSTL 250 260 270 280 290 300 TQASSMTPHS GPRSTTSQAS PAQRDTAQAA STREIPRASS PHRITQRDTS RASSTQQEIS 310 320 330 340 350 360 RASSTQQETS RASSTQEDTP RASSTQEDTP RASSTQWNTP RASSPSRSTQ LDNPRTSSTQ 370 380 390 400 410 420 QDNPQTSFPT CTPQRENPRT PCVQQDDPRA SSPNRTTQRE NSRTSCAQRD NPKASRTSSP 430 440 450 460 470 480 NRATRDNPRT SCAQRDNPRA SSPSRATRDN PTTSCAQRDN PRASRTSSPN RATRDNPRTS 490 500 510 520 530 540 CAQRDNPRAS SPSRATRDNP TTSCAQRDNP RASRTSSPNR ATRDNPRTSC AQRDNPRASS 550 560 570 580 590 600 PNRAARDNPT TSCAQRDNPR ASRTSSPNRA TRDNPRTSCA QRDNPRASSP NRATRDNPTT 610 620 630 640 650 660 SCAQRDNPRA SRTSSPNRAT RDNPRTSCAQ RDNPRASSPN RTTQQDSPRT SCARRDDPRA 670 680 690 700 710 720 SSPNRTIQQE NPRTSCALRD NPRASSPSRT IQQENPRTSC AQRDDPRASS PNRTTQQENP 730 740 750 760 770 780 RTSCARRDNP RASSRNRTIQ RDNPRTSCAQ RDNPRASSPN RTIQQENLRT SCTRQDNPRT 790 800 810 820 830 840 SSPNRATRDN PRTSCAQRDN LRASSPIRAT QQDNPRTCIQ QNIPRSSSTQ QDNPKTSCTK 850 860 870 880 890 900 RDNLRPTCTQ RDRTQSFSFQ RDNPGTSSSQ CCTQKENLRP SSPHRSTQWN NPRNSSPHRT 910 920 930 940 950 960 NKDIPWASFP LRPTQSDGPR TSSPSRSKQS EVPWASIALR PTQGDRPQTS SPSRPAQHDP 970 980 990 1000 1010 1020 PQSSFGPTQY NLPSRATSSS HNPGHQSTSR TSSPVYPAAY GAPLTSPEPS QPPCAVCIGH 1030 1040 1050 1060 1070 1080 RDAPRASSPP RYLQHDPFPF FPEPRAPESE PPHHEPPYIP PAVCIGHRDA PRASSPPRHT 1090 1100 1110 1120 1130 1140 QFDPFPFLPD TSDAEHQCQS PQHEPLQLPA PVCIGYRDAP RASSPPRQAP EPSLLFQDLP 1150 1160 1170 1180 1190 1200 RASTESLVPS MDSLHECPHI PTPVCIGHRD APSFSSPPRQ APEPSLFFQD PPGTSMESLA 1210 1220 1230 1240 1250 1260 PSTDSLHGSP VLIPQVCIGH RDAPRASSPP RHPPSDLAFL APSPSPGSSG GSRGSAPPGE 1270 1280 1290 1300 1310 1320 TRHNLEREEY TVLADLPPPR RLAQRQPGPQ AQCSSGGRTH SPGRAEVERL FGQERRKSEA 1330 1340 1350 1360 1370 1380 AGAFQAQDEG RSQQPSQGQS QLLRRQSSPA PSRQVTMLPA KQAELTRRSQ AEPPHPWSPE 1390 1400 1410 1420 1430 1440 KRPEGDRQLQ GSPLPPRTSA RTPERELRTQ RPLESGQAGP RQPLGVWQSQ EEPPGSQGPH 1450 1460 1470 1480 1490 1500 RHLERSWSSQ EGGLGPGGWW GCGEPSLGAA KAPEGAWGGT SREYKESWGQ PEAWEEKPTH 1510 1520 1530 1540 1550 1560 ELPRELGKRS PLTSPPENWG GPAESSQSWH SGTPTAVGWG AEGACPYPRG SERRPELDWR 1570 1580 1590 1600 1610 1620 DLLGLLRAPG EGVWARVPSL DWEGLLELLQ ARLPRKDPAG HRDDLARALG PELGPPGTND 1630 1640 1650 1660 1670 1680 VPEQESHSQP EGWAEATPVN GHSPALQSQS PVQLPSPACT STQWPKIKVT RGPATATLAG 1690 1700 1710 1720 1730 1740 LEQTGPLGSR STAKGPSLPE LQFQPEEPEE SEPSRGQDPL TDQKQADSAD KRPAEGKAGS 1750 1760 1770 1780 1790 1800 PLKGRLVTSW RMPGDRPTLF NPFLLSLGVL RWRRPDLLNF KKGWMSILDE PGEPPSPSLT 1810 1820 1830 1840 1850 1860 TTSTSQWKKH WFVLTDSSLK YYRDSTAEEA DELDGEIDLR SCTDVTEYAV QRNYGFQIHT 1870 1880 1890 1900 1910 1920 KDAVYTLSAM TSGIRRNWIE ALRKTVRPTS APDVTKLSDS NKENALHSYS TQKGPLKAGE 1930 1940 1950 1960 1970 1980 QRAGSEVISR GGPRKADGQR QALDYVELSP LTQASPQRAR TPARTPDRLA KQEELERDLA 1990 2000 2010 2020 2030 2040 QRSEERRKWF EATDSRTPEV PAGEGPRRGL GAPLTEDQQN RLSEEIEKKW QELEKLPLRE 2050 2060 2070 2080 2090 2100 NKRVPLTALL NQSRGERRGP PSDGHEALEK EVQALRAQLE AWRLQGEAPQ SALRSQEDGH 2110 2120 2130 2140 2150 2160 IPPGYISQEA CERSLAEMES SHQQVMEELQ RHHERELQRL QQEKEWLLAE ETAATASAIE 2170 2180 2190 2200 2210 2220 AMKKAYQEEL SRELSKTRSL QQGPDGLRKQ HQSDVEALKR ELQVLSEQYS QKCLEIGALM 2230 2240 2250 2260 2270 2280 RQAEEREHTL RRCQQEGQEL LRHNQELHGR LSEEIDQLRG FIASQGMGNG CGRSNERSSC 2290 2300 2310 2320 2330 2340 ELEVLLRVKE NELQYLKKEV QCLRDELQMM QKDKRFTSGK YQDVYVELSH IKTRSEREIE 2350 2360 QLKEHLRLAM AALQEKESMR NSLAE
	Isoform 3 (Long isoform) [UniParc]. Checksum: A0055359F5C3D655	FASTA	2,267	250,724
	10 20 30 40 50 60 MEEVPGDALC EHFEANILTQ NRCQNCFHPE EAHGARYQEL RSPSGAEVPY CDLPRCPPAP 70 80 90 100 110 120 EDPLSASTSG CQSVVDPGLR PGPKRGPSPS AGLPEEGPTA APRSRSRELE AVPYLEGLTT 130 140 150 160 170 180 SLCGSCNEDP GSDPTSSPDS ATPDDTSNSS SVDWDTVERQ EEEAPSWDEL AVMIPRRPRE 190 200 210 220 230 240 GPRADSSQRA PSLLTRSPVG GDAAGQKKED TGGGGRSAGQ HWARLRGESG LSLERHRSTL 250 260 270 280 290 300 TQASSMTPHS GPRSTTSQAS PAQRDTAQAA STREIPRASS PHRITQRDTS RASSTQQEIS 310 320 330 340 350 360 RASSTQQETS RASSTQEDTP RASSTQEDTP RASSTQWNTP RASSPSRSTQ LDNPRTSSTQ 370 380 390 400 410 420 QDNPQTSFPT CTPQRENPRT PCVQQDDPRA SSPNRTTQRE NSRTSCAQRD NPKASRTSSP 430 440 450 460 470 480 NRATRDNPRT SCAQRDNPRA SSPSRATRDN PTTSCAQRDN PRASRTSSPN RATRDNPRTS 490 500 510 520 530 540 CAQRDNPRAS SPSRATRDNP TTSCAQRDNP RASRTSSPNR ATRDNPRTSC AQRDNPRASS 550 560 570 580 590 600 PNRAARDNPT TSCAQRDNPR ASRTSSPNRA TRDNPRTSCA QRDNPRASSP NRATRDNPTT 610 620 630 640 650 660 SCAQRDNPRA SRTSSPNRAT RDNPRTSCAQ RDNPRASSPN RTTQQDSPRT SCARRDDPRA 670 680 690 700 710 720 SSPNRTIQQE NPRTSCALRD NPRASSPSRT IQQENPRTSC AQRDDPRASS PNRTTQQENP 730 740 750 760 770 780 RTSCARRDNP RASSRNRTIQ RDNPRTSCAQ RDNPRASSPN RTIQQENLRT SCTRQDNPRT 790 800 810 820 830 840 SSPNRATRDN PRTSCAQRDN LRASSPIRAT QQDNPRTCIQ QNIPRSSSTQ QDNPKTSCTK 850 860 870 880 890 900 RDNLRPTCTQ RDRTQSFSFQ RDNPGTSSSQ CCTQKENLRP SSPHRSTQWN NPRNSSPHRT 910 920 930 940 950 960 NKDIPWASFP LRPTQSDGPR TSSPSRSKQS EVPWASIALR PTQGDRPQTS SPSRPAQHDP 970 980 990 1000 1010 1020 PQSSFGPTQY NLPSRATSSS HNPGHQSTSR TSSPVYPAAY GAPLTSPEPS QPPCAVCIGH 1030 1040 1050 1060 1070 1080 RDAPRASSPP RYLQHDPFPF FPEPRAPESE PPHHEPPYIP PAVCIGHRDA PRASSPPRHT 1090 1100 1110 1120 1130 1140 QFDPFPFLPD TSDAEHQCQS PQHEPLQLPA PVCIGYRDAP RASSPPRQAP EPSLLFQDLP 1150 1160 1170 1180 1190 1200 RASTESLVPS MDSLHECPHI PTPVCIGHRD APSFSSPPRQ APEPSLFFQD PPGTSMESLA 1210 1220 1230 1240 1250 1260 PSTDSLHGSP VLIPQVCIGH RDAPRASSPP RHPPSDLAFL APSPSPGSSG GSRGSAPPGE 1270 1280 1290 1300 1310 1320 TRHNLEREEY TVLADLPPPR RLAQRQPGPQ AQCSSGGRTH SPGRAEVERL FGQERRTMLP 1330 1340 1350 1360 1370 1380 AKQAELTRRS QAEPPHPWSP EKRPEGDRQL QGSPLPPRTS ARTPERELRT QRPLESGQAG 1390 1400 1410 1420 1430 1440 PRQPLGVWQS QEEPPGSQGP HRHLERSWSS QEGGLGPGGW WGCGEPSLGA AKAPEGAWGG 1450 1460 1470 1480 1490 1500 TSREYKESWG QPEAWEEKPT HELPRELGKR SPLTSPPENW GGPAESSQSW HSGTPTAVGW 1510 1520 1530 1540 1550 1560 GAEGACPYPR GSERRPELDW RDLLGLLRAP GEGVWARVPS LDWEGLLELL QARLPRKDPA 1570 1580 1590 1600 1610 1620 GHRDDLARAL GPELGPPGTN DVPEQESHSQ PEGWAEATPV NGHSPALQSQ SPVQLPSPAC 1630 1640 1650 1660 1670 1680 TSTQWPKIKV TRGPATATLA GLEQTGPLGS RSTAKGPSLP ELQFQPEEPE ESEPSRGQDP 1690 1700 1710 1720 1730 1740 LTDQKQADSP DLLNFKKGWM SILDEPGEWK KHWFVLTDSS LKYYRDSTAE EADELDGEID 1750 1760 1770 1780 1790 1800 LRSCTDVTEY AVQRNYGFQI HTKDAVYTLS AMTSGIRRNW IEALRKTVRP TSAPDVTKLS 1810 1820 1830 1840 1850 1860 DSNKENALHS YSTQKGPLKA GEQRAGSEVI SRGGPRKADG QRQALDYVEL SPLTQASPQR 1870 1880 1890 1900 1910 1920 ARTPARTPDR LAKQEELERD LAQRSEERRK WFEATDSRTP EVPAGEGPRR GLGAPLTEDQ 1930 1940 1950 1960 1970 1980 QNRLSEEIEK KWQELEKLPL RENKRVPLTA LLNQSRGERR GPPSDGHEAL EKEVQALRAQ 1990 2000 2010 2020 2030 2040 LEAWRLQGEA PQSALRSQED GHIPPGYISQ EACERSLAEM ESSHQQVMEE LQRHHERELQ 2050 2060 2070 2080 2090 2100 RLQQEKEWLL AEETAATASA IEAMKKAYQE ELSRELSKTR SLQQGPDGLR KQHQSDVEAL 2110 2120 2130 2140 2150 2160 KRELQVLSEQ YSQKCLEIGA LMRQAEEREH TLRRCQQEGQ ELLRHNQELH GRLSEEIDQL 2170 2180 2190 2200 2210 2220 RGFIASQGMG NGCGRSNERS SCELEVLLRV KENELQYLKK EVQCLRDELQ MMQKDKRFTS 2230 2240 2250 2260 GKYQDVYVEL SHIKTRSERE IEQLKEHLRL AMAALQEKES MRNSLAE
	Isoform 4 (TRIOBP-3) [UniParc]. Checksum: 2056F789C0E11E01	FASTA	2,193	243,071
	10 20 30 40 50 60 MIPRRPREGP RADSSQRAPS LLTRSPVGGD AAGQKKEDTG GGGRSAGQHW ARLRGESGLS 70 80 90 100 110 120 LERHRSTLTQ ASSMTPHSGP RSTTSQASPA QRDTAQAAST REIPRASSPH RITQRDTSRA 130 140 150 160 170 180 SSTQQEISRA SSTQQETSRA SSTQEDTPRA SSTQEDTPRA SSTQWNTPRA SSPSRSTQLD 190 200 210 220 230 240 NPRTSSTQQD NPQTSFPTCT PQRENPRTPC VQQDDPRASS PNRTTQRENS RTSCAQRDNP 250 260 270 280 290 300 KASRTSSPNR ATRDNPRTSC AQRDNPRASS PSRATRDNPT TSCAQRDNPR ASRTSSPNRA 310 320 330 340 350 360 TRDNPRTSCA QRDNPRASSP SRATRDNPTT SCAQRDNPRA SRTSSPNRAT RDNPRTSCAQ 370 380 390 400 410 420 RDNPRASSPN RAARDNPTTS CAQRDNPRAS RTSSPNRATR DNPRTSCAQR DNPRASSPNR 430 440 450 460 470 480 ATRDNPTTSC AQRDNPRASR TSSPNRATRD NPRTSCAQRD NPRASSPNRT TQQDSPRTSC 490 500 510 520 530 540 ARRDDPRASS PNRTIQQENP RTSCALRDNP RASSPSRTIQ QENPRTSCAQ RDDPRASSPN 550 560 570 580 590 600 RTTQQENPRT SCARRDNPRA SSRNRTIQRD NPRTSCAQRD NPRASSPNRT IQQENLRTSC 610 620 630 640 650 660 TRQDNPRTSS PNRATRDNPR TSCAQRDNLR ASSPIRATQQ DNPRTCIQQN IPRSSSTQQD 670 680 690 700 710 720 NPKTSCTKRD NLRPTCTQRD RTQSFSFQRD NPGTSSSQCC TQKENLRPSS PHRSTQWNNP 730 740 750 760 770 780 RNSSPHRTNK DIPWASFPLR PTQSDGPRTS SPSRSKQSEV PWASIALRPT QGDRPQTSSP 790 800 810 820 830 840 SRPAQHDPPQ SSFGPTQYNL PSRATSSSHN PGHQSTSRTS SPVYPAAYGA PLTSPEPSQP 850 860 870 880 890 900 PCAVCIGHRD APRASSPPRY LQHDPFPFFP EPRAPESEPP HHEPPYIPPA VCIGHRDAPR 910 920 930 940 950 960 ASSPPRHTQF DPFPFLPDTS DAEHQCQSPQ HEPLQLPAPV CIGYRDAPRA SSPPRQAPEP 970 980 990 1000 1010 1020 SLLFQDLPRA STESLVPSMD SLHECPHIPT PVCIGHRDAP SFSSPPRQAP EPSLFFQDPP 1030 1040 1050 1060 1070 1080 GTSMESLAPS TDSLHGSPVL IPQVCIGHRD APRASSPPRH PPSDLAFLAP SPSPGSSGGS 1090 1100 1110 1120 1130 1140 RGSAPPGETR HNLEREEYTV LADLPPPRRL AQRQPGPQAQ CSSGGRTHSP GRAEVERLFG 1150 1160 1170 1180 1190 1200 QERRKSEAAG AFQAQDEGRS QQPSQGQSQL LRRQSSPAPS RQVTMLPAKQ AELTRRSQAE 1210 1220 1230 1240 1250 1260 PPHPWSPEKR PEGDRQLQGS PLPPRTSART PERELRTQRP LESGQAGPRQ PLGVWQSQEE 1270 1280 1290 1300 1310 1320 PPGSQGPHRH LERSWSSQEG GLGPGGWWGC GEPSLGAAKA PEGAWGGTSR EYKESWGQPE 1330 1340 1350 1360 1370 1380 AWEEKPTHEL PRELGKRSPL TSPPENWGGP AESSQSWHSG TPTAVGWGAE GACPYPRGSE 1390 1400 1410 1420 1430 1440 RRPELDWRDL LGLLRAPGEG VWARVPSLDW EGLLELLQAR LPRKDPAGHR DDLARALGPE 1450 1460 1470 1480 1490 1500 LGPPGTNDVP EQESHSQPEG WAEATPVNGH SPALQSQSPV QLPSPACTST QWPKIKVTRG 1510 1520 1530 1540 1550 1560 PATATLAGLE QTGPLGSRST AKGPSLPELQ FQPEEPEESE PSRGQDPLTD QKQADSADKR 1570 1580 1590 1600 1610 1620 PAEGKAGSPL KGRLVTSWRM PGDRPTLFNP FLLSLGVLRW RRPDLLNFKK GWMSILDEPG 1630 1640 1650 1660 1670 1680 EPPSPSLTTT STSQWKKHWF VLTDSSLKYY RDSTAEEADE LDGEIDLRSC TDVTEYAVQR 1690 1700 1710 1720 1730 1740 NYGFQIHTKD AVYTLSAMTS GIRRNWIEAL RKTVRPTSAP DVTKLSDSNK ENALHSYSTQ 1750 1760 1770 1780 1790 1800 KGPLKAGEQR AGSEVISRGG PRKADGQRQA LDYVELSPLT QASPQRARTP ARTPDRLAKQ 1810 1820 1830 1840 1850 1860 EELERDLAQR SEERRKWFEA TDSRTPEVPA GEGPRRGLGA PLTEDQQNRL SEEIEKKWQE 1870 1880 1890 1900 1910 1920 LEKLPLRENK RVPLTALLNQ SRGERRGPPS DGHEALEKEV QALRAQLEAW RLQGEAPQSA 1930 1940 1950 1960 1970 1980 LRSQEDGHIP PGYISQEACE RSLAEMESSH QQVMEELQRH HERELQRLQQ EKEWLLAEET 1990 2000 2010 2020 2030 2040 AATASAIEAM KKAYQEELSR ELSKTRSLQQ GPDGLRKQHQ SDVEALKREL QVLSEQYSQK 2050 2060 2070 2080 2090 2100 CLEIGALMRQ AEEREHTLRR CQQEGQELLR HNQELHGRLS EEIDQLRGFI ASQGMGNGCG 2110 2120 2130 2140 2150 2160 RSNERSSCEL EVLLRVKENE LQYLKKEVQC LRDELQMMQK DKRFTSGKYQ DVYVELSHIK 2170 2180 2190 TRSEREIEQL KEHLRLAMAA LQEKESMRNS LAE
	Isoform 5 (TRIOBP-4) [UniParc]. Checksum: 90E403C1C0B946FD	FASTA	1,144	125,524
	10 20 30 40 50 60 MIPRRPREGP RADSSQRAPS LLTRSPVGGD AAGQKKEDTG GGGRSAGQHW ARLRGESGLS 70 80 90 100 110 120 LERHRSTLTQ ASSMTPHSGP RSTTSQASPA QRDTAQAAST REIPRASSPH RITQRDTSRA 130 140 150 160 170 180 SSTQQEISRA SSTQQETSRA SSTQEDTPRA SSTQEDTPRA SSTQWNTPRA SSPSRSTQLD 190 200 210 220 230 240 NPRTSSTQQD NPQTSFPTCT PQRENPRTPC VQQDDPRASS PNRTTQRENS RTSCAQRDNP 250 260 270 280 290 300 KASRTSSPNR ATRDNPRTSC AQRDNPRASS PSRATRDNPT TSCAQRDNPR ASRTSSPNRA 310 320 330 340 350 360 TRDNPRTSCA QRDNPRASSP SRATRDNPTT SCAQRDNPRA SRTSSPNRAT RDNPRTSCAQ 370 380 390 400 410 420 RDNPRASSPN RAARDNPTTS CAQRDNPRAS RTSSPNRATR DNPRTSCAQR DNPRASSPNR 430 440 450 460 470 480 ATRDNPTTSC AQRDNPRASR TSSPNRATRD NPRTSCAQRD NPRASSPNRT TQQDSPRTSC 490 500 510 520 530 540 ARRDDPRASS PNRTIQQENP RTSCALRDNP RASSPSRTIQ QENPRTSCAQ RDDPRASSPN 550 560 570 580 590 600 RTTQQENPRT SCARRDNPRA SSRNRTIQRD NPRTSCAQRD NPRASSPNRT IQQENLRTSC 610 620 630 640 650 660 TRQDNPRTSS PNRATRDNPR TSCAQRDNLR ASSPIRATQQ DNPRTCIQQN IPRSSSTQQD 670 680 690 700 710 720 NPKTSCTKRD NLRPTCTQRD RTQSFSFQRD NPGTSSSQCC TQKENLRPSS PHRSTQWNNP 730 740 750 760 770 780 RNSSPHRTNK DIPWASFPLR PTQSDGPRTS SPSRSKQSEV PWASIALRPT QGDRPQTSSP 790 800 810 820 830 840 SRPAQHDPPQ SSFGPTQYNL PSRATSSSHN PGHQSTSRTS SPVYPAAYGA PLTSPEPSQP 850 860 870 880 890 900 PCAVCIGHRD APRASSPPRY LQHDPFPFFP EPRAPESEPP HHEPPYIPPA VCIGHRDAPR 910 920 930 940 950 960 ASSPPRHTQF DPFPFLPDTS DAEHQCQSPQ HEPLQLPAPV CIGYRDAPRA SSPPRQAPEP 970 980 990 1000 1010 1020 SLLFQDLPRA STESLVPSMD SLHECPHIPT PVCIGHRDAP SFSSPPRQAP EPSLFFQDPP 1030 1040 1050 1060 1070 1080 GTSMESLAPS TDSLHGSPVL IPQVCIGHRD APRASSPPRH PPSDLAFLAP SPSPGSSGGS 1090 1100 1110 1120 1130 1140 RGSAPPGETR HNLEREEYTV LADLPPPRRL AQRQPGPQAQ CSSGGRTHSP GRAEVERLFG QERR
	Isoform 1 (TRIOBP-1) [UniParc]. Checksum: 109AA7D9CC10B21A	FASTA	593	68,041
	10 20 30 40 50 60 MTPDLLNFKK GWMSILDEPG EPPSPSLTTT STSQWKKHWF VLTDSSLKYY RDSTAEEADE 70 80 90 100 110 120 LDGEIDLRSC TDVTEYAVQR NYGFQIHTKD AVYTLSAMTS GIRRNWIEAL RKTVRPTSAP 130 140 150 160 170 180 DVTKLSDSNK ENALHSYSTQ KGPLKAGEQR AGSEVISRGG PRKADGQRQA LDYVELSPLT 190 200 210 220 230 240 QASPQRARTP ARTPDRLAKQ EELERDLAQR SEERRKWFEA TDSRTPEVPA GEGPRRGLGA 250 260 270 280 290 300 PLTEDQQNRL SEEIEKKWQE LEKLPLRENK RVPLTALLNQ SRGERRGPPS DGHEALEKEV 310 320 330 340 350 360 QALRAQLEAW RLQGEAPQSA LRSQEDGHIP PGYISQEACE RSLAEMESSH QQVMEELQRH 370 380 390 400 410 420 HERELQRLQQ EKEWLLAEET AATASAIEAM KKAYQEELSR ELSKTRSLQQ GPDGLRKQHQ 430 440 450 460 470 480 SDVEALKREL QVLSEQYSQK CLEIGALMRQ AEEREHTLRR CQQEGQELLR HNQELHGRLS 490 500 510 520 530 540 EEIDQLRGFI ASQGMGNGCG RSNERSSCEL EVLLRVKENE LQYLKKEVQC LRDELQMMQK 550 560 570 580 590 DKRFTSGKYQ DVYVELSHIK TRSEREIEQL KEHLRLAMAA LQEKESMRNS LAE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Tara, a novel F-actin binding protein, associates with the Trio guanine nucleotide exchange factor and regulates actin cytoskeletal organization." Seipel K., O'Brien S.P., Iannotti E., Medley Q.G., Streuli M. J. Cell Sci. 114:389-399(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]	"Mutations in TRIOBP, which encodes a putative cytoskeletal-organizing protein, are associated with nonsyndromic recessive deafness." Riazuddin S., Khan S.N., Ahmed Z.M., Ghosh M., Caution K., Nazli S., Kabra M., Zafar A.U., Chen K., Naz S., Antonellis A., Pavan W.J., Green E.D., Wilcox E.R., Friedman P.L., Morell R.J., Riazuddin S., Friedman T.B. Am. J. Hum. Genet. 78:137-143(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 2; 4 AND 5), INVOLVEMENT IN DFNB28. Tissue: Inner ear.
[3]	"Mutations in a novel isoform of TRIOBP that encodes a filamentous-actin binding protein are responsible for DFNB28 recessive nonsyndromic hearing loss." Shahin H., Walsh T., Sobe T., Abu Sa'ed J., Abu Rayan A., Lynch E.D., Lee M.K., Avraham K.B., King M.-C., Kanaan M. Am. J. Hum. Genet. 78:144-152(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3), TISSUE SPECIFICITY, VARIANTS DFNB28 ARG-1019 AND ARG-1377. Tissue: Brain.
[4]	"The DNA sequence of human chromosome 22." Dunham I., Hunt A.R., Collins J.E., Bruskiewich R., Beare D.M., Clamp M., Smink L.J., Ainscough R., Almeida J.P., Babbage A.K., Bagguley C., Bailey J., Barlow K.F., Bates K.N., Beasley O.P., Bird C.P., Blakey S.E., Bridgeman A.M. , Buck D., Burgess J., Burrill W.D., Burton J., Carder C., Carter N.P., Chen Y., Clark G., Clegg S.M., Cobley V.E., Cole C.G., Collier R.E., Connor R., Conroy D., Corby N.R., Coville G.J., Cox A.V., Davis J., Dawson E., Dhami P.D., Dockree C., Dodsworth S.J., Durbin R.M., Ellington A.G., Evans K.L., Fey J.M., Fleming K., French L., Garner A.A., Gilbert J.G.R., Goward M.E., Grafham D.V., Griffiths M.N.D., Hall C., Hall R.E., Hall-Tamlyn G., Heathcott R.W., Ho S., Holmes S., Hunt S.E., Jones M.C., Kershaw J., Kimberley A.M., King A., Laird G.K., Langford C.F., Leversha M.A., Lloyd C., Lloyd D.M., Martyn I.D., Mashreghi-Mohammadi M., Matthews L.H., Mccann O.T., Mcclay J., Mclaren S., McMurray A.A., Milne S.A., Mortimore B.J., Odell C.N., Pavitt R., Pearce A.V., Pearson D., Phillimore B.J.C.T., Phillips S.H., Plumb R.W., Ramsay H., Ramsey Y., Rogers L., Ross M.T., Scott C.E., Sehra H.K., Skuce C.D., Smalley S., Smith M.L., Soderlund C., Spragon L., Steward C.A., Sulston J.E., Swann R.M., Vaudin M., Wall M., Wallis J.M., Whiteley M.N., Willey D.L., Williams L., Williams S.A., Williamson H., Wilmer T.E., Wilming L., Wright C.L., Hubbard T., Bentley D.R., Beck S., Rogers J., Shimizu N., Minoshima S., Kawasaki K., Sasaki T., Asakawa S., Kudoh J., Shintani A., Shibuya K., Yoshizaki Y., Aoki N., Mitsuyama S., Roe B.A., Chen F., Chu L., Crabtree J., Deschamps S., Do A., Do T., Dorman A., Fang F., Fu Y., Hu P., Hua A., Kenton S., Lai H., Lao H.I., Lewis J., Lewis S., Lin S.-P., Loh P., Malaj E., Nguyen T., Pan H., Phan S., Qi S., Qian Y., Ray L., Ren Q., Shaull S., Sloan D., Song L., Wang Q., Wang Y., Wang Z., White J., Willingham D., Wu H., Yao Z., Zhan M., Zhang G., Chissoe S., Murray J., Miller N., Minx P., Fulton R., Johnson D., Bemis G., Bentley D., Bradshaw H., Bourne S., Cordes M., Du Z., Fulton L., Goela D., Graves T., Hawkins J., Hinds K., Kemp K., Latreille P., Layman D., Ozersky P., Rohlfing T., Scheet P., Walker C., Wamsley A., Wohldmann P., Pepin K., Nelson J., Korf I., Bedell J.A., Hillier L.W., Mardis E., Waterston R., Wilson R., Emanuel B.S., Shaikh T., Kurahashi H., Saitta S., Budarf M.L., McDermid H.E., Johnson A., Wong A.C.C., Morrow B.E., Edelmann L., Kim U.J., Shizuya H., Simon M.I., Dumanski J.P., Peyrard M., Kedra D., Seroussi E., Fransson I., Tapia I., Bruder C.E., O'Brien K.P., Wilkinson P., Bodenteich A., Hartman K., Hu X., Khan A.S., Lane L., Tilahun Y., Wright H. Nature 402:489-495(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Colon and Pancreas.
[6]	"Selection system for genes encoding nuclear-targeted proteins." Ueki N., Oda T., Kondo M., Yano K., Noguchi T., Muramatsu M.-A. Nat. Biotechnol. 16:1338-1342(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 340-563, SUBCELLULAR LOCATION. Tissue: Fetal brain.
[7]	"Identification of novel transcribed sequences on human chromosome 22 by expressed sequence tag mapping." Hirosawa M., Nagase T., Murahashi Y., Kikuno R., Ohara O. DNA Res. 8:1-9(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 713-2365, VARIANT ARG-1377. Tissue: Brain.
[8]	"Construction of expression-ready cDNA clones for KIAA genes: manual curation of 330 KIAA cDNA clones." Nakajima D., Okazaki N., Yamakawa H., Kikuno R., Ohara O., Nagase T. DNA Res. 9:99-106(2002) [PubMed] [Europe PMC] [Abstract] Cited for: SEQUENCE REVISION.
[9]	"The E3 ubiquitin ligase HECTD3 regulates ubiquitination and degradation of Tara." Yu J., Lan J., Zhu Y., Li X., Lai X., Xue Y., Jin C., Huang H. Biochem. Biophys. Res. Commun. 367:805-812(2008) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH HECTD3, UBIQUITINATION.
[10]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1949 AND SER-1955, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[11]	"Large-scale proteomics analysis of the human kinome." Oppermann F.S., Gnad F., Olsen J.V., Hornberger R., Greff Z., Keri G., Mann M., Daub H. Mol. Cell. Proteomics 8:1751-1764(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1955, MASS SPECTROMETRY.
[12]	"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis." Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M. Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1949 AND SER-1955, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[13]	"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation." Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B. Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF281030 mRNA. Translation: AAG44841.1. DQ228003 mRNA. Translation: ABB59559.1. DQ228004 mRNA. Translation: ABB59560.1. DQ228005 mRNA. Translation: ABB59561.1. DQ278603 mRNA. Translation: ABB77204.1. Z83844 Genomic DNA. Translation: CAQ09783.1. Z83844 Genomic DNA. Translation: CAQ09786.1. BC003618 mRNA. Translation: AAH03618.1. BC004303 mRNA. Translation: AAH04303.1. BC013278 mRNA. Translation: AAH13278.2. AB015343 mRNA. Translation: BAA34800.1. Frameshift. AB051449 mRNA. Translation: BAB33332.2.
IPI	IPI00148768. IPI00413160. IPI00736522. IPI00741684. IPI00952918.
RefSeq	NP_001034230.1. NM_001039141.2. NP_008963.3. NM_007032.5. NP_619538.2. NM_138632.2.
UniGene	Hs.533030.
3D structure databases
ProteinModelPortal	Q9H2D6.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9H2D6. 8 interactions.
MINT	MINT-1397991.
PTM databases
PhosphoSite	Q9H2D6.
Polymorphism databases
DMDM	90110075.
Proteomic databases
PaxDb	Q9H2D6.
PRIDE	Q9H2D6.
Protocols and materials databases
DNASU	11078.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000406386; ENSP00000384312; ENSG00000100106.
GeneID	11078.
KEGG	hsa:11078.
UCSC	uc003atq.1. human. uc003ats.1. human.
Organism-specific databases
CTD	11078.
GeneCards	GC22P038092.
HGNC	HGNC:17009. TRIOBP.
HPA	HPA003747. HPA019769.
MIM	609761. gene. 609823. phenotype.
neXtProt	NX_Q9H2D6.
Orphanet	90636. Autosomal recessive nonsyndromic sensorineural deafness type DFNB.
PharmGKB	PA142670699.
HUGE	Search...
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG12793.
HOVERGEN	HBG094025.
InParanoid	Q9H2D6.
OMA	SPNRATR.
OrthoDB	EOG4RFKST.
Gene expression databases
ArrayExpress	Q9H2D6.
Bgee	Q9H2D6.
Genevestigator	Q9H2D6.
GermOnline	ENSG00000100106. Homo sapiens.
Family and domain databases
Gene3D	2.30.29.30. 1 hit.
InterPro	IPR011993. PH_like_dom. IPR001849. Pleckstrin_homology. [Graphical view]
Pfam	PF00169. PH. 1 hit. [Graphical view]
SMART	SM00233. PH. 1 hit. [Graphical view]
PROSITE	PS50003. PH_DOMAIN. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	TRIOBP. human.
GenomeRNAi	11078.
NextBio	42114.
SOURCE	Search...

Entry information

Entry name

TARA_HUMAN

Accession

Primary (citable) accession number: Q9H2D6
Secondary accession number(s): B1AHD4 , B1AHD7, O94797, Q2PZW8, Q2Q3Z9, Q2Q400, Q5R3M6, Q96DW1, Q9BT77, Q9BTL7, Q9BY98, Q9Y3L4

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 2, 2002
Last sequence update:	March 21, 2006
Last modified:	May 1, 2013
This is version 117 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.