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Q9H223 (EHD4_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 127. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
EH domain-containing protein 4
Alternative name(s):
Hepatocellular carcinoma-associated protein 10/11
PAST homolog 4
Gene names
Name:EHD4
Synonyms:HCA10, HCA11, PAST4
ORF Names:FKSG7
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length541 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Plays a role in early endosomal transport. Ref.8 Ref.10

Subunit structure

Homooligomer, and heterooligomer with EHD1, EHD2 and EHD3. Ref.8

Subcellular location

Cell membrane By similarity. Early endosome membrane; Peripheral membrane protein. Recycling endosome membrane; Peripheral membrane protein Ref.10.

Tissue specificity

Highly expressed in pancreas and heart.

Domain

The EH domain interacts with Asn-Pro-Phe (NPF) motifs of target proteins By similarity.

Sequence similarities

Belongs to the TRAFAC class dynamin-like GTPase superfamily. Dynamin/Fzo/YdjA family. EHD subfamily.

Contains 1 dynamin-type G (guanine nucleotide-binding) domain.

Contains 1 EF-hand domain.

Contains 1 EH domain.

Ontologies

Keywords
   Cellular componentCell membrane
Endosome
Membrane
   Coding sequence diversityPolymorphism
   LigandATP-binding
Calcium
Metal-binding
Nucleotide-binding
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcellular response to growth factor stimulus

Inferred from electronic annotation. Source: Ensembl

endocytic recycling

Inferred from genetic interaction Ref.8. Source: UniProtKB

pinocytosis

Inferred from electronic annotation. Source: Ensembl

positive regulation of peptidyl-tyrosine phosphorylation

Inferred from electronic annotation. Source: Ensembl

protein homooligomerization

Inferred from physical interaction Ref.8. Source: UniProtKB

regulation of endocytosis

Inferred from electronic annotation. Source: Ensembl

   Cellular_componentearly endosome membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

endoplasmic reticulum

Inferred from direct assay. Source: LIFEdb

extracellular vesicular exosome

Inferred from direct assay PubMed 19056867PubMed 20458337PubMed 23376485. Source: UniProt

nucleus

Traceable author statement Ref.5. Source: ProtInc

perinuclear region of cytoplasm

Inferred from electronic annotation. Source: Ensembl

plasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

recycling endosome membrane

Inferred from direct assay Ref.8. Source: UniProtKB

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

GTP binding

Inferred from electronic annotation. Source: InterPro

GTPase activity

Inferred from electronic annotation. Source: InterPro

calcium ion binding

Inferred from electronic annotation. Source: InterPro

nucleic acid binding

Traceable author statement Ref.5. Source: ProtInc

protein binding

Inferred from physical interaction Ref.8. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 541541EH domain-containing protein 4
PRO_0000146114

Regions

Domain58 – 289232Dynamin-type G
Domain447 – 53589EH
Domain479 – 51436EF-hand
Nucleotide binding68 – 758ATP By similarity
Calcium binding492 – 50312 By similarity

Sites

Binding site2231ATP By similarity
Binding site2611ATP By similarity

Amino acid modifications

Modified residue4511Phosphotyrosine Ref.6
Modified residue4591Phosphoserine Ref.9 Ref.11

Natural variations

Natural variant1541V → I.
Corresponds to variant rs11549015 [ dbSNP | Ensembl ].
VAR_053070

Experimental info

Sequence conflict2971L → I in AAG28784. Ref.2
Sequence conflict421 – 43313TEGPF…YGEGA → PRAPSTRATGRVP in AAG28784. Ref.2
Sequence conflict4391E → Q in AAG28784. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Q9H223 [UniParc].

Last modified March 1, 2001. Version 1.
Checksum: 72DDE551829B7BF5

FASTA54161,175
        10         20         30         40         50         60 
MFSWMGRQAG GRERAGGADA VQTVTGGLRS LYLRKVLPLE EAYRFHEFHS PALEDADFEN 

        70         80         90        100        110        120 
KPMILLVGQY STGKTTFIRY LLEQDFPGMR IGPEPTTDSF IAVMYGETEG STPGNALVVD 

       130        140        150        160        170        180 
PKKPFRKLSR FGNAFLNRFM CSQLPNQVLK SISVIDSPGI LSGEKQRISR GYDFCQVLQW 

       190        200        210        220        230        240 
FAERVDRIIL LFDAHKLDIS DEFSEAIKAF RGQDDKIRVV LNKADQVDTQ QLMRVYGALM 

       250        260        270        280        290        300 
WSLGKVINTP EVLRVYIGSF WAQPLQNTDN RRLFEAEAQD LFRDIQSLPQ KAAVRKLNDL 

       310        320        330        340        350        360 
IKRARLAKVH AYIISYLKKE MPSVFGKENK KRELISRLPE IYIQLQREYQ ISAGDFPEVK 

       370        380        390        400        410        420 
AMQEQLENYD FTKFHSLKPK LIEAVDNMLS NKISPLMNLI SQEETSTPTQ LVQGGAFDGT 

       430        440        450        460        470        480 
TEGPFNQGYG EGAKEGADEE EWVVAKDKPV YDELFYTLSP INGKISGVNA KKEMVTSKLP 

       490        500        510        520        530        540 
NSVLGKIWKL ADCDCDGMLD EEEFALAKHL IKIKLDGYEL PSSLPPHLVP PSHRKSLPKA 


D 

« Hide

References

« Hide 'large scale' references
[1]"Large scale identification of human hepatocellular carcinoma-associated antigens by autoantibodies."
Wang Y., Han K.-J., Pang X.-W., Vaughan H.A., Qu W., Dong X.-Y., Peng J.-R., Zhao H.-T., Rui J.-A., Leng X.-S., Cebon J., Burgess A.W., Chen W.-F.
J. Immunol. 169:1102-1109(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Hepatoma.
[2]"Cloning of FKSG7, a novel gene which encodes an EH domain-containing protein."
Wang Y.-G.
Submitted (SEP-2000) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Brain.
[3]"hEHD4, an EH domain containing protein-4."
Benjamin S., Horowitz M.
Submitted (DEC-2001) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Brain and Placenta.
[5]"EHD2, EHD3, and EHD4 encode novel members of a highly conserved family of EH domain-containing proteins."
Pohl U., Smith J.S., Tachibana I., Ueki K., Lee H.K., Ramaswamy S., Wu Q., Mohrenweiser H.W., Jenkins R.B., Louis D.N.
Genomics 63:255-262(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 60-541.
Tissue: Brain.
[6]"Immunoaffinity profiling of tyrosine phosphorylation in cancer cells."
Rush J., Moritz A., Lee K.A., Guo A., Goss V.L., Spek E.J., Zhang H., Zha X.-M., Polakiewicz R.D., Comb M.J.
Nat. Biotechnol. 23:94-101(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-451, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[7]"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."
Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M.
Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[8]"Shared as well as distinct roles of EHD proteins revealed by biochemical and functional comparisons in mammalian cells and C. elegans."
George M., Ying G., Rainey M.A., Solomon A., Parikh P.T., Gao Q., Band V., Band H.
BMC Cell Biol. 8:3-3(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBUNIT.
[9]"A quantitative atlas of mitotic phosphorylation."
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-459, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[10]"A role for EHD4 in the regulation of early endosomal transport."
Sharma M., Naslavsky N., Caplan S.
Traffic 9:995-1018(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH EHD1 AND EHD3, SUBCELLULAR LOCATION.
[11]"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-459, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[12]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF323924 mRNA. Translation: AAK11599.1.
AF307137 mRNA. Translation: AAG28784.1.
AF454953 mRNA. Translation: AAL51079.1.
BC006287 mRNA. Translation: AAH06287.1.
BC051823 mRNA. Translation: AAH51823.1.
AF181265 mRNA. Translation: AAF40472.1.
CCDSCCDS10081.1.
RefSeqNP_644670.1. NM_139265.3.
UniGeneHs.143703.

3D structure databases

ProteinModelPortalQ9H223.
SMRQ9H223. Positions 1-535.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid119054. 21 interactions.
IntActQ9H223. 5 interactions.
MINTMINT-5003543.
STRING9606.ENSP00000220325.

PTM databases

PhosphoSiteQ9H223.

Polymorphism databases

DMDM18202935.

Proteomic databases

MaxQBQ9H223.
PaxDbQ9H223.
PeptideAtlasQ9H223.
PRIDEQ9H223.

Protocols and materials databases

DNASU30844.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000220325; ENSP00000220325; ENSG00000103966.
GeneID30844.
KEGGhsa:30844.
UCSCuc001zot.3. human.

Organism-specific databases

CTD30844.
GeneCardsGC15M042190.
HGNCHGNC:3245. EHD4.
HPAHPA049890.
HPA049986.
HPA052729.
MIM605892. gene.
neXtProtNX_Q9H223.
PharmGKBPA27680.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG136252.
HOGENOMHOG000242040.
HOVERGENHBG018183.
InParanoidQ9H223.
KOK12477.
OMAMPNQVLQ.
OrthoDBEOG757CX9.
PhylomeDBQ9H223.
TreeFamTF314429.

Gene expression databases

BgeeQ9H223.
CleanExHS_EHD4.
GenevestigatorQ9H223.

Family and domain databases

Gene3D1.10.238.10. 1 hit.
3.40.50.300. 3 hits.
InterProIPR001401. Dynamin_GTPase.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR000261. EPS15_homology.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamPF00350. Dynamin_N. 1 hit.
[Graphical view]
SMARTSM00027. EH. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 1 hit.
PROSITEPS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 1 hit.
PS50031. EH. 1 hit.
PS51718. G_DYNAMIN_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiEHD4.
GenomeRNAi30844.
NextBio52964.
PROQ9H223.
SOURCESearch...

Entry information

Entry nameEHD4_HUMAN
AccessionPrimary (citable) accession number: Q9H223
Secondary accession number(s): Q9HAR1, Q9NZN2
Entry history
Integrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: March 1, 2001
Last modified: July 9, 2014
This is version 127 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 15

Human chromosome 15: entries, gene names and cross-references to MIM