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Q9H223

- EHD4_HUMAN

UniProt

Q9H223 - EHD4_HUMAN

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Protein

EH domain-containing protein 4

Gene

EHD4

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Plays a role in early endosomal transport.2 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei223 – 2231ATPBy similarity
Binding sitei261 – 2611ATPBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi68 – 758ATPBy similarity
Calcium bindingi492 – 50312PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. calcium ion binding Source: InterPro
  3. GTPase activity Source: InterPro
  4. GTP binding Source: InterPro
  5. nucleic acid binding Source: ProtInc

GO - Biological processi

  1. cellular response to growth factor stimulus Source: Ensembl
  2. endocytic recycling Source: UniProtKB
  3. pinocytosis Source: Ensembl
  4. positive regulation of peptidyl-tyrosine phosphorylation Source: Ensembl
  5. protein homooligomerization Source: UniProtKB
  6. regulation of endocytosis Source: Ensembl
Complete GO annotation...

Keywords - Ligandi

ATP-binding, Calcium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
EH domain-containing protein 4
Alternative name(s):
Hepatocellular carcinoma-associated protein 10/11
PAST homolog 4
Gene namesi
Name:EHD4
Synonyms:HCA10, HCA11, PAST4
ORF Names:FKSG7
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 15

Organism-specific databases

HGNCiHGNC:3245. EHD4.

Subcellular locationi

Cell membrane By similarity. Early endosome membrane 1 Publication; Peripheral membrane protein 1 Publication. Recycling endosome membrane 1 Publication; Peripheral membrane protein 1 Publication

GO - Cellular componenti

  1. endoplasmic reticulum Source: LIFEdb
  2. extracellular vesicular exosome Source: UniProtKB
  3. membrane Source: UniProtKB
  4. nucleus Source: ProtInc
  5. perinuclear region of cytoplasm Source: Ensembl
  6. plasma membrane Source: UniProtKB-KW
  7. recycling endosome membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Endosome, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA27680.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 541541EH domain-containing protein 4PRO_0000146114Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei451 – 4511Phosphotyrosine1 Publication
Modified residuei459 – 4591Phosphoserine2 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ9H223.
PaxDbiQ9H223.
PeptideAtlasiQ9H223.
PRIDEiQ9H223.

PTM databases

PhosphoSiteiQ9H223.

Expressioni

Tissue specificityi

Highly expressed in pancreas and heart.

Gene expression databases

BgeeiQ9H223.
CleanExiHS_EHD4.
GenevestigatoriQ9H223.

Organism-specific databases

HPAiHPA049890.
HPA049986.
HPA052729.

Interactioni

Subunit structurei

Homooligomer, and heterooligomer with EHD1, EHD2 and EHD3.1 Publication

Protein-protein interaction databases

BioGridi119054. 26 interactions.
IntActiQ9H223. 5 interactions.
MINTiMINT-5003543.
STRINGi9606.ENSP00000220325.

Structurei

3D structure databases

ProteinModelPortaliQ9H223.
SMRiQ9H223. Positions 1-535.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini58 – 289232Dynamin-type GAdd
BLAST
Domaini447 – 53589EHPROSITE-ProRule annotationAdd
BLAST
Domaini479 – 51436EF-handPROSITE-ProRule annotationAdd
BLAST

Domaini

The EH domain interacts with Asn-Pro-Phe (NPF) motifs of target proteins.By similarity

Sequence similaritiesi

Contains 1 EF-hand domain.PROSITE-ProRule annotation
Contains 1 EH domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG136252.
GeneTreeiENSGT00760000118985.
HOGENOMiHOG000242040.
HOVERGENiHBG018183.
InParanoidiQ9H223.
KOiK12477.
OMAiMPNQVLQ.
OrthoDBiEOG757CX9.
PhylomeDBiQ9H223.
TreeFamiTF314429.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
3.40.50.300. 3 hits.
InterProiIPR001401. Dynamin_GTPase.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR000261. EPS15_homology.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00350. Dynamin_N. 1 hit.
[Graphical view]
SMARTiSM00027. EH. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 1 hit.
PS50031. EH. 1 hit.
PS51718. G_DYNAMIN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9H223-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MFSWMGRQAG GRERAGGADA VQTVTGGLRS LYLRKVLPLE EAYRFHEFHS
60 70 80 90 100
PALEDADFEN KPMILLVGQY STGKTTFIRY LLEQDFPGMR IGPEPTTDSF
110 120 130 140 150
IAVMYGETEG STPGNALVVD PKKPFRKLSR FGNAFLNRFM CSQLPNQVLK
160 170 180 190 200
SISVIDSPGI LSGEKQRISR GYDFCQVLQW FAERVDRIIL LFDAHKLDIS
210 220 230 240 250
DEFSEAIKAF RGQDDKIRVV LNKADQVDTQ QLMRVYGALM WSLGKVINTP
260 270 280 290 300
EVLRVYIGSF WAQPLQNTDN RRLFEAEAQD LFRDIQSLPQ KAAVRKLNDL
310 320 330 340 350
IKRARLAKVH AYIISYLKKE MPSVFGKENK KRELISRLPE IYIQLQREYQ
360 370 380 390 400
ISAGDFPEVK AMQEQLENYD FTKFHSLKPK LIEAVDNMLS NKISPLMNLI
410 420 430 440 450
SQEETSTPTQ LVQGGAFDGT TEGPFNQGYG EGAKEGADEE EWVVAKDKPV
460 470 480 490 500
YDELFYTLSP INGKISGVNA KKEMVTSKLP NSVLGKIWKL ADCDCDGMLD
510 520 530 540
EEEFALAKHL IKIKLDGYEL PSSLPPHLVP PSHRKSLPKA D
Length:541
Mass (Da):61,175
Last modified:March 1, 2001 - v1
Checksum:i72DDE551829B7BF5
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti297 – 2971L → I in AAG28784. 1 PublicationCurated
Sequence conflicti421 – 43313TEGPF…YGEGA → PRAPSTRATGRVP in AAG28784. 1 PublicationCuratedAdd
BLAST
Sequence conflicti439 – 4391E → Q in AAG28784. 1 PublicationCurated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti154 – 1541V → I.
Corresponds to variant rs11549015 [ dbSNP | Ensembl ].
VAR_053070

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF323924 mRNA. Translation: AAK11599.1.
AF307137 mRNA. Translation: AAG28784.1.
AF454953 mRNA. Translation: AAL51079.1.
BC006287 mRNA. Translation: AAH06287.1.
BC051823 mRNA. Translation: AAH51823.1.
AF181265 mRNA. Translation: AAF40472.1.
CCDSiCCDS10081.1.
RefSeqiNP_644670.1. NM_139265.3.
UniGeneiHs.143703.

Genome annotation databases

EnsembliENST00000220325; ENSP00000220325; ENSG00000103966.
GeneIDi30844.
KEGGihsa:30844.
UCSCiuc001zot.3. human.

Polymorphism databases

DMDMi18202935.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF323924 mRNA. Translation: AAK11599.1 .
AF307137 mRNA. Translation: AAG28784.1 .
AF454953 mRNA. Translation: AAL51079.1 .
BC006287 mRNA. Translation: AAH06287.1 .
BC051823 mRNA. Translation: AAH51823.1 .
AF181265 mRNA. Translation: AAF40472.1 .
CCDSi CCDS10081.1.
RefSeqi NP_644670.1. NM_139265.3.
UniGenei Hs.143703.

3D structure databases

ProteinModelPortali Q9H223.
SMRi Q9H223. Positions 1-535.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 119054. 26 interactions.
IntActi Q9H223. 5 interactions.
MINTi MINT-5003543.
STRINGi 9606.ENSP00000220325.

PTM databases

PhosphoSitei Q9H223.

Polymorphism databases

DMDMi 18202935.

Proteomic databases

MaxQBi Q9H223.
PaxDbi Q9H223.
PeptideAtlasi Q9H223.
PRIDEi Q9H223.

Protocols and materials databases

DNASUi 30844.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000220325 ; ENSP00000220325 ; ENSG00000103966 .
GeneIDi 30844.
KEGGi hsa:30844.
UCSCi uc001zot.3. human.

Organism-specific databases

CTDi 30844.
GeneCardsi GC15M042190.
HGNCi HGNC:3245. EHD4.
HPAi HPA049890.
HPA049986.
HPA052729.
MIMi 605892. gene.
neXtProti NX_Q9H223.
PharmGKBi PA27680.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG136252.
GeneTreei ENSGT00760000118985.
HOGENOMi HOG000242040.
HOVERGENi HBG018183.
InParanoidi Q9H223.
KOi K12477.
OMAi MPNQVLQ.
OrthoDBi EOG757CX9.
PhylomeDBi Q9H223.
TreeFami TF314429.

Miscellaneous databases

GeneWikii EHD4.
GenomeRNAii 30844.
NextBioi 52964.
PROi Q9H223.
SOURCEi Search...

Gene expression databases

Bgeei Q9H223.
CleanExi HS_EHD4.
Genevestigatori Q9H223.

Family and domain databases

Gene3Di 1.10.238.10. 1 hit.
3.40.50.300. 3 hits.
InterProi IPR001401. Dynamin_GTPase.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR000261. EPS15_homology.
IPR027417. P-loop_NTPase.
[Graphical view ]
Pfami PF00350. Dynamin_N. 1 hit.
[Graphical view ]
SMARTi SM00027. EH. 1 hit.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 1 hit.
PROSITEi PS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 1 hit.
PS50031. EH. 1 hit.
PS51718. G_DYNAMIN_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Large scale identification of human hepatocellular carcinoma-associated antigens by autoantibodies."
    Wang Y., Han K.-J., Pang X.-W., Vaughan H.A., Qu W., Dong X.-Y., Peng J.-R., Zhao H.-T., Rui J.-A., Leng X.-S., Cebon J., Burgess A.W., Chen W.-F.
    J. Immunol. 169:1102-1109(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Hepatoma.
  2. "Cloning of FKSG7, a novel gene which encodes an EH domain-containing protein."
    Wang Y.-G.
    Submitted (SEP-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Brain.
  3. "hEHD4, an EH domain containing protein-4."
    Benjamin S., Horowitz M.
    Submitted (DEC-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain and Placenta.
  5. "EHD2, EHD3, and EHD4 encode novel members of a highly conserved family of EH domain-containing proteins."
    Pohl U., Smith J.S., Tachibana I., Ueki K., Lee H.K., Ramaswamy S., Wu Q., Mohrenweiser H.W., Jenkins R.B., Louis D.N.
    Genomics 63:255-262(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 60-541.
    Tissue: Brain.
  6. "Immunoaffinity profiling of tyrosine phosphorylation in cancer cells."
    Rush J., Moritz A., Lee K.A., Guo A., Goss V.L., Spek E.J., Zhang H., Zha X.-M., Polakiewicz R.D., Comb M.J.
    Nat. Biotechnol. 23:94-101(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-451, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  7. "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."
    Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M.
    Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  8. "Shared as well as distinct roles of EHD proteins revealed by biochemical and functional comparisons in mammalian cells and C. elegans."
    George M., Ying G., Rainey M.A., Solomon A., Parikh P.T., Gao Q., Band V., Band H.
    BMC Cell Biol. 8:3-3(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBUNIT.
  9. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-459, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  10. "A role for EHD4 in the regulation of early endosomal transport."
    Sharma M., Naslavsky N., Caplan S.
    Traffic 9:995-1018(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH EHD1 AND EHD3, SUBCELLULAR LOCATION.
  11. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
    Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
    Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-459, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  12. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiEHD4_HUMAN
AccessioniPrimary (citable) accession number: Q9H223
Secondary accession number(s): Q9HAR1, Q9NZN2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: March 1, 2001
Last modified: October 29, 2014
This is version 129 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 15
    Human chromosome 15: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3