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Protein

EH domain-containing protein 4

Gene

EHD4

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

ATP- and membrane-binding protein that probably controls membrane reorganization/tubulation upon ATP hydrolysis. Plays a role in early endosomal transport.2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei223ATPBy similarity1
Binding sitei261ATPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi68 – 75ATPBy similarity8
Calcium bindingi492 – 503PROSITE-ProRule annotationBy similarityAdd BLAST12

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • cadherin binding Source: BHF-UCL
  • calcium ion binding Source: InterPro
  • GTP binding Source: InterPro
  • nucleic acid binding Source: ProtInc

GO - Biological processi

  • cellular response to growth factor stimulus Source: Ensembl
  • endocytic recycling Source: UniProtKB
  • pinocytosis Source: Ensembl
  • positive regulation of peptidyl-tyrosine phosphorylation Source: Ensembl
  • protein homooligomerization Source: UniProtKB
  • regulation of endocytosis Source: Ensembl

Keywordsi

LigandATP-binding, Calcium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
EH domain-containing protein 4Curated
Alternative name(s):
Hepatocellular carcinoma-associated protein 10/111 Publication
PAST homolog 4Curated
Gene namesi
Name:EHD4Imported
Synonyms:HCA101 Publication, HCA111 Publication, PAST4Imported
ORF Names:FKSG7Imported
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 15

Organism-specific databases

EuPathDBiHostDB:ENSG00000103966.9
HGNCiHGNC:3245 EHD4
MIMi605892 gene
neXtProtiNX_Q9H223

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Endosome, Membrane

Pathology & Biotechi

Organism-specific databases

OpenTargetsiENSG00000103966
PharmGKBiPA27680

Polymorphism and mutation databases

BioMutaiEHD4
DMDMi18202935

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001461141 – 541EH domain-containing protein 4Add BLAST541

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei162PhosphoserineCombined sources1
Modified residuei451PhosphotyrosineCombined sources1
Modified residuei459PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9H223
MaxQBiQ9H223
PaxDbiQ9H223
PeptideAtlasiQ9H223
PRIDEiQ9H223

PTM databases

iPTMnetiQ9H223
PhosphoSitePlusiQ9H223

Expressioni

Tissue specificityi

Highly expressed in pancreas and heart.1 Publication

Gene expression databases

BgeeiENSG00000103966
CleanExiHS_EHD4
ExpressionAtlasiQ9H223 baseline and differential
GenevisibleiQ9H223 HS

Organism-specific databases

HPAiHPA049890
HPA049986
HPA052729
HPA066751

Interactioni

Subunit structurei

Homooligomer, and heterooligomer with EHD1, EHD2 and EHD3.1 Publication

GO - Molecular functioni

  • cadherin binding Source: BHF-UCL

Protein-protein interaction databases

BioGridi11905461 interactors.
IntActiQ9H223 15 interactors.
MINTiQ9H223
STRINGi9606.ENSP00000220325

Structurei

3D structure databases

ProteinModelPortaliQ9H223
SMRiQ9H223
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini58 – 289Dynamin-type GAdd BLAST232
Domaini447 – 535EHPROSITE-ProRule annotationAdd BLAST89
Domaini479 – 514EF-handPROSITE-ProRule annotationAdd BLAST36

Domaini

The EH domain interacts with Asn-Pro-Phe (NPF) motifs of target proteins.By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1954 Eukaryota
ENOG410XYGB LUCA
GeneTreeiENSGT00760000118985
HOGENOMiHOG000242040
HOVERGENiHBG018183
InParanoidiQ9H223
KOiK12477
OMAiGEMYQTV
OrthoDBiEOG091G04KA
PhylomeDBiQ9H223
TreeFamiTF314429

Family and domain databases

InterProiView protein in InterPro
IPR022812 Dynamin_SF
IPR011992 EF-hand-dom_pair
IPR018247 EF_Hand_1_Ca_BS
IPR002048 EF_hand_dom
IPR000261 EH_dom
IPR029952 EHD4
IPR031692 EHD_N
IPR030381 G_DYNAMIN_dom
IPR027417 P-loop_NTPase
PANTHERiPTHR11216:SF57 PTHR11216:SF57, 1 hit
PfamiView protein in Pfam
PF00350 Dynamin_N, 1 hit
PF12763 EF-hand_4, 1 hit
PF16880 EHD_N, 1 hit
SMARTiView protein in SMART
SM00027 EH, 1 hit
SUPFAMiSSF47473 SSF47473, 1 hit
SSF52540 SSF52540, 1 hit
PROSITEiView protein in PROSITE
PS00018 EF_HAND_1, 1 hit
PS50222 EF_HAND_2, 1 hit
PS50031 EH, 1 hit
PS51718 G_DYNAMIN_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q9H223-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFSWMGRQAG GRERAGGADA VQTVTGGLRS LYLRKVLPLE EAYRFHEFHS
60 70 80 90 100
PALEDADFEN KPMILLVGQY STGKTTFIRY LLEQDFPGMR IGPEPTTDSF
110 120 130 140 150
IAVMYGETEG STPGNALVVD PKKPFRKLSR FGNAFLNRFM CSQLPNQVLK
160 170 180 190 200
SISVIDSPGI LSGEKQRISR GYDFCQVLQW FAERVDRIIL LFDAHKLDIS
210 220 230 240 250
DEFSEAIKAF RGQDDKIRVV LNKADQVDTQ QLMRVYGALM WSLGKVINTP
260 270 280 290 300
EVLRVYIGSF WAQPLQNTDN RRLFEAEAQD LFRDIQSLPQ KAAVRKLNDL
310 320 330 340 350
IKRARLAKVH AYIISYLKKE MPSVFGKENK KRELISRLPE IYIQLQREYQ
360 370 380 390 400
ISAGDFPEVK AMQEQLENYD FTKFHSLKPK LIEAVDNMLS NKISPLMNLI
410 420 430 440 450
SQEETSTPTQ LVQGGAFDGT TEGPFNQGYG EGAKEGADEE EWVVAKDKPV
460 470 480 490 500
YDELFYTLSP INGKISGVNA KKEMVTSKLP NSVLGKIWKL ADCDCDGMLD
510 520 530 540
EEEFALAKHL IKIKLDGYEL PSSLPPHLVP PSHRKSLPKA D
Length:541
Mass (Da):61,175
Last modified:March 1, 2001 - v1
Checksum:i72DDE551829B7BF5
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti297L → I in AAG28784 (Ref. 2) Curated1
Sequence conflicti421 – 433TEGPF…YGEGA → PRAPSTRATGRVP in AAG28784 (Ref. 2) CuratedAdd BLAST13
Sequence conflicti439E → Q in AAG28784 (Ref. 2) Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_053070154V → I. Corresponds to variant dbSNP:rs11549015Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF323924 mRNA Translation: AAK11599.1
AF307137 mRNA Translation: AAG28784.1
AF454953 mRNA Translation: AAL51079.1
BC006287 mRNA Translation: AAH06287.1
BC051823 mRNA Translation: AAH51823.1
AF181265 mRNA Translation: AAF40472.1
CCDSiCCDS10081.1
RefSeqiNP_644670.1, NM_139265.3
UniGeneiHs.143703

Genome annotation databases

EnsembliENST00000220325; ENSP00000220325; ENSG00000103966
GeneIDi30844
KEGGihsa:30844
UCSCiuc001zot.4 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiEHD4_HUMAN
AccessioniPrimary (citable) accession number: Q9H223
Secondary accession number(s): Q9HAR1, Q9NZN2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: March 1, 2001
Last modified: March 28, 2018
This is version 162 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome