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Q9H223

- EHD4_HUMAN

UniProt

Q9H223 - EHD4_HUMAN

Protein

EH domain-containing protein 4

Gene

EHD4

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 128 (01 Oct 2014)
      Sequence version 1 (01 Mar 2001)
      Previous versions | rss
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    Functioni

    Plays a role in early endosomal transport.2 Publications

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei223 – 2231ATPBy similarity
    Binding sitei261 – 2611ATPBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi68 – 758ATPBy similarity
    Calcium bindingi492 – 50312PROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. calcium ion binding Source: InterPro
    3. GTPase activity Source: InterPro
    4. GTP binding Source: InterPro
    5. nucleic acid binding Source: ProtInc
    6. protein binding Source: UniProtKB

    GO - Biological processi

    1. cellular response to growth factor stimulus Source: Ensembl
    2. endocytic recycling Source: UniProtKB
    3. pinocytosis Source: Ensembl
    4. positive regulation of peptidyl-tyrosine phosphorylation Source: Ensembl
    5. protein homooligomerization Source: UniProtKB
    6. regulation of endocytosis Source: Ensembl

    Keywords - Ligandi

    ATP-binding, Calcium, Metal-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    EH domain-containing protein 4
    Alternative name(s):
    Hepatocellular carcinoma-associated protein 10/11
    PAST homolog 4
    Gene namesi
    Name:EHD4
    Synonyms:HCA10, HCA11, PAST4
    ORF Names:FKSG7
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 15

    Organism-specific databases

    HGNCiHGNC:3245. EHD4.

    Subcellular locationi

    Cell membrane By similarity. Early endosome membrane 1 Publication; Peripheral membrane protein 1 Publication. Recycling endosome membrane 1 Publication; Peripheral membrane protein 1 Publication

    GO - Cellular componenti

    1. early endosome membrane Source: UniProtKB-SubCell
    2. endoplasmic reticulum Source: LIFEdb
    3. extracellular vesicular exosome Source: UniProt
    4. membrane Source: UniProtKB
    5. nucleus Source: ProtInc
    6. perinuclear region of cytoplasm Source: Ensembl
    7. plasma membrane Source: UniProtKB-SubCell
    8. recycling endosome membrane Source: UniProtKB

    Keywords - Cellular componenti

    Cell membrane, Endosome, Membrane

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA27680.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 541541EH domain-containing protein 4PRO_0000146114Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei451 – 4511Phosphotyrosine1 Publication
    Modified residuei459 – 4591Phosphoserine2 Publications

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ9H223.
    PaxDbiQ9H223.
    PeptideAtlasiQ9H223.
    PRIDEiQ9H223.

    PTM databases

    PhosphoSiteiQ9H223.

    Expressioni

    Tissue specificityi

    Highly expressed in pancreas and heart.

    Gene expression databases

    BgeeiQ9H223.
    CleanExiHS_EHD4.
    GenevestigatoriQ9H223.

    Organism-specific databases

    HPAiHPA049890.
    HPA049986.
    HPA052729.

    Interactioni

    Subunit structurei

    Homooligomer, and heterooligomer with EHD1, EHD2 and EHD3.1 Publication

    Protein-protein interaction databases

    BioGridi119054. 21 interactions.
    IntActiQ9H223. 5 interactions.
    MINTiMINT-5003543.
    STRINGi9606.ENSP00000220325.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9H223.
    SMRiQ9H223. Positions 1-535.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini58 – 289232Dynamin-type GAdd
    BLAST
    Domaini447 – 53589EHPROSITE-ProRule annotationAdd
    BLAST
    Domaini479 – 51436EF-handPROSITE-ProRule annotationAdd
    BLAST

    Domaini

    The EH domain interacts with Asn-Pro-Phe (NPF) motifs of target proteins.By similarity

    Sequence similaritiesi

    Contains 1 EF-hand domain.PROSITE-ProRule annotation
    Contains 1 EH domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG136252.
    HOGENOMiHOG000242040.
    HOVERGENiHBG018183.
    InParanoidiQ9H223.
    KOiK12477.
    OMAiMPNQVLQ.
    OrthoDBiEOG757CX9.
    PhylomeDBiQ9H223.
    TreeFamiTF314429.

    Family and domain databases

    Gene3Di1.10.238.10. 1 hit.
    3.40.50.300. 3 hits.
    InterProiIPR001401. Dynamin_GTPase.
    IPR011992. EF-hand-dom_pair.
    IPR018247. EF_Hand_1_Ca_BS.
    IPR002048. EF_hand_dom.
    IPR000261. EPS15_homology.
    IPR027417. P-loop_NTPase.
    [Graphical view]
    PfamiPF00350. Dynamin_N. 1 hit.
    [Graphical view]
    SMARTiSM00027. EH. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 1 hit.
    PROSITEiPS00018. EF_HAND_1. 1 hit.
    PS50222. EF_HAND_2. 1 hit.
    PS50031. EH. 1 hit.
    PS51718. G_DYNAMIN_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9H223-1 [UniParc]FASTAAdd to Basket

    « Hide

    MFSWMGRQAG GRERAGGADA VQTVTGGLRS LYLRKVLPLE EAYRFHEFHS    50
    PALEDADFEN KPMILLVGQY STGKTTFIRY LLEQDFPGMR IGPEPTTDSF 100
    IAVMYGETEG STPGNALVVD PKKPFRKLSR FGNAFLNRFM CSQLPNQVLK 150
    SISVIDSPGI LSGEKQRISR GYDFCQVLQW FAERVDRIIL LFDAHKLDIS 200
    DEFSEAIKAF RGQDDKIRVV LNKADQVDTQ QLMRVYGALM WSLGKVINTP 250
    EVLRVYIGSF WAQPLQNTDN RRLFEAEAQD LFRDIQSLPQ KAAVRKLNDL 300
    IKRARLAKVH AYIISYLKKE MPSVFGKENK KRELISRLPE IYIQLQREYQ 350
    ISAGDFPEVK AMQEQLENYD FTKFHSLKPK LIEAVDNMLS NKISPLMNLI 400
    SQEETSTPTQ LVQGGAFDGT TEGPFNQGYG EGAKEGADEE EWVVAKDKPV 450
    YDELFYTLSP INGKISGVNA KKEMVTSKLP NSVLGKIWKL ADCDCDGMLD 500
    EEEFALAKHL IKIKLDGYEL PSSLPPHLVP PSHRKSLPKA D 541
    Length:541
    Mass (Da):61,175
    Last modified:March 1, 2001 - v1
    Checksum:i72DDE551829B7BF5
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti297 – 2971L → I in AAG28784. 1 PublicationCurated
    Sequence conflicti421 – 43313TEGPF…YGEGA → PRAPSTRATGRVP in AAG28784. 1 PublicationCuratedAdd
    BLAST
    Sequence conflicti439 – 4391E → Q in AAG28784. 1 PublicationCurated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti154 – 1541V → I.
    Corresponds to variant rs11549015 [ dbSNP | Ensembl ].
    VAR_053070

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF323924 mRNA. Translation: AAK11599.1.
    AF307137 mRNA. Translation: AAG28784.1.
    AF454953 mRNA. Translation: AAL51079.1.
    BC006287 mRNA. Translation: AAH06287.1.
    BC051823 mRNA. Translation: AAH51823.1.
    AF181265 mRNA. Translation: AAF40472.1.
    CCDSiCCDS10081.1.
    RefSeqiNP_644670.1. NM_139265.3.
    UniGeneiHs.143703.

    Genome annotation databases

    EnsembliENST00000220325; ENSP00000220325; ENSG00000103966.
    GeneIDi30844.
    KEGGihsa:30844.
    UCSCiuc001zot.3. human.

    Polymorphism databases

    DMDMi18202935.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF323924 mRNA. Translation: AAK11599.1 .
    AF307137 mRNA. Translation: AAG28784.1 .
    AF454953 mRNA. Translation: AAL51079.1 .
    BC006287 mRNA. Translation: AAH06287.1 .
    BC051823 mRNA. Translation: AAH51823.1 .
    AF181265 mRNA. Translation: AAF40472.1 .
    CCDSi CCDS10081.1.
    RefSeqi NP_644670.1. NM_139265.3.
    UniGenei Hs.143703.

    3D structure databases

    ProteinModelPortali Q9H223.
    SMRi Q9H223. Positions 1-535.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 119054. 21 interactions.
    IntActi Q9H223. 5 interactions.
    MINTi MINT-5003543.
    STRINGi 9606.ENSP00000220325.

    PTM databases

    PhosphoSitei Q9H223.

    Polymorphism databases

    DMDMi 18202935.

    Proteomic databases

    MaxQBi Q9H223.
    PaxDbi Q9H223.
    PeptideAtlasi Q9H223.
    PRIDEi Q9H223.

    Protocols and materials databases

    DNASUi 30844.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000220325 ; ENSP00000220325 ; ENSG00000103966 .
    GeneIDi 30844.
    KEGGi hsa:30844.
    UCSCi uc001zot.3. human.

    Organism-specific databases

    CTDi 30844.
    GeneCardsi GC15M042190.
    HGNCi HGNC:3245. EHD4.
    HPAi HPA049890.
    HPA049986.
    HPA052729.
    MIMi 605892. gene.
    neXtProti NX_Q9H223.
    PharmGKBi PA27680.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG136252.
    HOGENOMi HOG000242040.
    HOVERGENi HBG018183.
    InParanoidi Q9H223.
    KOi K12477.
    OMAi MPNQVLQ.
    OrthoDBi EOG757CX9.
    PhylomeDBi Q9H223.
    TreeFami TF314429.

    Miscellaneous databases

    GeneWikii EHD4.
    GenomeRNAii 30844.
    NextBioi 52964.
    PROi Q9H223.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q9H223.
    CleanExi HS_EHD4.
    Genevestigatori Q9H223.

    Family and domain databases

    Gene3Di 1.10.238.10. 1 hit.
    3.40.50.300. 3 hits.
    InterProi IPR001401. Dynamin_GTPase.
    IPR011992. EF-hand-dom_pair.
    IPR018247. EF_Hand_1_Ca_BS.
    IPR002048. EF_hand_dom.
    IPR000261. EPS15_homology.
    IPR027417. P-loop_NTPase.
    [Graphical view ]
    Pfami PF00350. Dynamin_N. 1 hit.
    [Graphical view ]
    SMARTi SM00027. EH. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 1 hit.
    PROSITEi PS00018. EF_HAND_1. 1 hit.
    PS50222. EF_HAND_2. 1 hit.
    PS50031. EH. 1 hit.
    PS51718. G_DYNAMIN_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Large scale identification of human hepatocellular carcinoma-associated antigens by autoantibodies."
      Wang Y., Han K.-J., Pang X.-W., Vaughan H.A., Qu W., Dong X.-Y., Peng J.-R., Zhao H.-T., Rui J.-A., Leng X.-S., Cebon J., Burgess A.W., Chen W.-F.
      J. Immunol. 169:1102-1109(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Hepatoma.
    2. "Cloning of FKSG7, a novel gene which encodes an EH domain-containing protein."
      Wang Y.-G.
      Submitted (SEP-2000) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Brain.
    3. "hEHD4, an EH domain containing protein-4."
      Benjamin S., Horowitz M.
      Submitted (DEC-2001) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Brain and Placenta.
    5. "EHD2, EHD3, and EHD4 encode novel members of a highly conserved family of EH domain-containing proteins."
      Pohl U., Smith J.S., Tachibana I., Ueki K., Lee H.K., Ramaswamy S., Wu Q., Mohrenweiser H.W., Jenkins R.B., Louis D.N.
      Genomics 63:255-262(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 60-541.
      Tissue: Brain.
    6. "Immunoaffinity profiling of tyrosine phosphorylation in cancer cells."
      Rush J., Moritz A., Lee K.A., Guo A., Goss V.L., Spek E.J., Zhang H., Zha X.-M., Polakiewicz R.D., Comb M.J.
      Nat. Biotechnol. 23:94-101(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-451, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    7. "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."
      Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M.
      Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    8. "Shared as well as distinct roles of EHD proteins revealed by biochemical and functional comparisons in mammalian cells and C. elegans."
      George M., Ying G., Rainey M.A., Solomon A., Parikh P.T., Gao Q., Band V., Band H.
      BMC Cell Biol. 8:3-3(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBUNIT.
    9. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-459, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    10. "A role for EHD4 in the regulation of early endosomal transport."
      Sharma M., Naslavsky N., Caplan S.
      Traffic 9:995-1018(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH EHD1 AND EHD3, SUBCELLULAR LOCATION.
    11. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
      Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
      Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-459, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    12. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiEHD4_HUMAN
    AccessioniPrimary (citable) accession number: Q9H223
    Secondary accession number(s): Q9HAR1, Q9NZN2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 26, 2001
    Last sequence update: March 1, 2001
    Last modified: October 1, 2014
    This is version 128 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 15
      Human chromosome 15: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3