UniProtKB - Q9H204 (MED28_HUMAN)
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Protein
Mediator of RNA polymerase II transcription subunit 28
Gene
MED28
Organism
Homo sapiens (Human)
Status
Functioni
Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. May be part of a complex containing NF2/merlin that participates in cellular signaling to the actin cytoskeleton downstream of tyrosine kinase signaling pathways.1 Publication
GO - Molecular functioni
- actin binding Source: UniProtKB-KW
GO - Biological processi
- negative regulation of smooth muscle cell differentiation Source: Ensembl
- regulation of transcription, DNA-templated Source: UniProtKB-KW
- stem cell population maintenance Source: Ensembl
- transcription, DNA-templated Source: UniProtKB-KW
Keywordsi
Molecular function | Actin-binding, Activator |
Biological process | Transcription, Transcription regulation |
Enzyme and pathway databases
Reactomei | R-HSA-1989781. PPARA activates gene expression. R-HSA-381340. Transcriptional regulation of white adipocyte differentiation. |
SIGNORi | Q9H204. |
Names & Taxonomyi
Protein namesi | Recommended name: Mediator of RNA polymerase II transcription subunit 28Alternative name(s): Endothelial-derived protein 1 Mediator complex subunit 28 Merlin and Grb2-interacting cytoskeletal protein Short name: Magicin Tumor angiogenesis marker EG-1 |
Gene namesi | Name:MED28 Synonyms:EG1 ORF Names:FKSG20 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000118579.11. |
HGNCi | HGNC:24628. MED28. |
MIMi | 610311. gene. |
neXtProti | NX_Q9H204. |
Pathology & Biotechi
Organism-specific databases
DisGeNETi | 80306. |
OpenTargetsi | ENSG00000118579. |
PharmGKBi | PA134935853. |
Polymorphism and mutation databases
BioMutai | MED28. |
DMDMi | 59799838. |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000113981 | 1 – 178 | Mediator of RNA polymerase II transcription subunit 28Add BLAST | 178 |
Proteomic databases
EPDi | Q9H204. |
MaxQBi | Q9H204. |
PaxDbi | Q9H204. |
PeptideAtlasi | Q9H204. |
PRIDEi | Q9H204. |
PTM databases
iPTMneti | Q9H204. |
PhosphoSitePlusi | Q9H204. |
Expressioni
Tissue specificityi
Widely expressed. Highly expressed in vascular tissues such as placenta, testis and liver.1 Publication
Inductioni
Up-regulated by endothelial cells when exposed to tumor conditional media.1 Publication
Gene expression databases
Bgeei | ENSG00000118579. |
CleanExi | HS_MED28. |
ExpressionAtlasi | Q9H204. baseline and differential. |
Genevisiblei | Q9H204. HS. |
Organism-specific databases
HPAi | HPA035900. HPA035901. |
Interactioni
Subunit structurei
Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP. Forms a ternary complex with NF2/merlin and GRB2. Binds to actin.2 Publications
Binary interactionsi
GO - Molecular functioni
- actin binding Source: UniProtKB-KW
Protein-protein interaction databases
BioGridi | 123219. 86 interactors. |
CORUMi | Q9H204. |
IntActi | Q9H204. 57 interactors. |
MINTi | Q9H204. |
STRINGi | 9606.ENSP00000237380. |
Structurei
3D structure databases
ProteinModelPortali | Q9H204. |
SMRi | Q9H204. |
ModBasei | Search... |
MobiDBi | Search... |
Family & Domainsi
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 109 – 145 | Sequence analysisAdd BLAST | 37 |
Sequence similaritiesi
Belongs to the Mediator complex subunit 28 family.Curated
Keywords - Domaini
Coiled coilPhylogenomic databases
eggNOGi | ENOG410IETV. Eukaryota. ENOG4111HUA. LUCA. |
GeneTreei | ENSGT00390000006192. |
HOGENOMi | HOG000113540. |
HOVERGENi | HBG052448. |
InParanoidi | Q9H204. |
KOi | K15141. |
PhylomeDBi | Q9H204. |
TreeFami | TF326988. |
Family and domain databases
InterProi | View protein in InterPro IPR021640. Mediator_Med28. |
PANTHERi | PTHR13512. PTHR13512. 1 hit. |
i Sequence
Sequence statusi: Complete.
Q9H204-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAAPLGGMFS GQPPGPPQAP PGLPGQASLL QAAPGAPRPS SSTLVDELES
60 70 80 90 100
SFEACFASLV SQDYVNGTDQ EEIRTGVDQC IQKFLDIARQ TECFFLQKRL
110 120 130 140 150
QLSVQKPEQV IKEDVSELRN ELQRKDALVQ KHLTKLRHWQ QVLEDINVQH
160 170
KKPADIPQGS LAYLEQASAN IPAPLKPT
Sequence cautioni
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF321617 mRNA. Translation: AAG38612.1. AF358829 mRNA. Translation: AAK32724.1. AF318059 mRNA. Translation: AAL26906.1. AF317680 mRNA. Translation: AAK11563.1. Frameshift. BC011936 mRNA. Translation: AAH11936.1. |
CCDSi | CCDS33963.1. |
RefSeqi | NP_079481.2. NM_025205.4. |
UniGenei | Hs.731966. |
Genome annotation databases
Ensembli | ENST00000237380; ENSP00000237380; ENSG00000118579. |
GeneIDi | 80306. |
KEGGi | hsa:80306. |
UCSCi | uc003gpk.2. human. |
Similar proteinsi
Entry informationi
Entry namei | MED28_HUMAN | |
Accessioni | Q9H204Primary (citable) accession number: Q9H204 Secondary accession number(s): Q9BZJ5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 2005 |
Last sequence update: | March 1, 2001 | |
Last modified: | February 28, 2018 | |
This is version 137 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |