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Q9H204 (MED28_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 108. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Mediator of RNA polymerase II transcription subunit 28
Alternative name(s):
Endothelial-derived protein 1
Mediator complex subunit 28
Merlin and Grb2-interacting cytoskeletal protein
Short name=Magicin
Tumor angiogenesis marker EG-1
Gene names
Name:MED28
Synonyms:EG1
ORF Names:FKSG20
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length178 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. May be part of a complex containing NF2/merlin that participates in cellular signaling to the actin cytoskeleton downstream of tyrosine kinase signaling pathways. Ref.6

Subunit structure

Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP. Forms a ternary complex with NF2/merlin and GRB2. Binds to actin. Ref.5 Ref.6 Ref.7

Subcellular location

Nucleus. Cytoplasm. Membrane; Peripheral membrane protein. Note: According to Ref.6, it is cytoplasmic and mainly membrane-associated. Ref.6

Tissue specificity

Widely expressed. Highly expressed in vascular tissues such as placenta, testis and liver. Ref.6

Induction

Up-regulated by endothelial cells when exposed to tumor conditional media. Ref.1

Sequence similarities

Belongs to the Mediator complex subunit 28 family.

Sequence caution

The sequence AAK11563.1 differs from that shown. Reason: Frameshift at position 165.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 178178Mediator of RNA polymerase II transcription subunit 28
PRO_0000113981

Regions

Coiled coil109 – 14537 Potential

Sequences

Sequence LengthMass (Da)Tools
Q9H204 [UniParc].

Last modified March 1, 2001. Version 1.
Checksum: 63DE023972908066

FASTA17819,520
        10         20         30         40         50         60 
MAAPLGGMFS GQPPGPPQAP PGLPGQASLL QAAPGAPRPS SSTLVDELES SFEACFASLV 

        70         80         90        100        110        120 
SQDYVNGTDQ EEIRTGVDQC IQKFLDIARQ TECFFLQKRL QLSVQKPEQV IKEDVSELRN 

       130        140        150        160        170 
ELQRKDALVQ KHLTKLRHWQ QVLEDINVQH KKPADIPQGS LAYLEQASAN IPAPLKPT 

« Hide

References

« Hide 'large scale' references
[1]"Identification of a novel endothelial-derived gene EG-1."
Liu C., Zhang L., Shao Z.-M., Beatty P., Sartippour M., Lane T.F., Barsky S.H., Livingston E., Nguyen M.H.
Biochem. Biophys. Res. Commun. 290:602-612(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], INDUCTION.
[2]"Cloning of FKSG20, a novel gene expressed in adrenal gland tumor tissues."
Wang Y.-G., Gong L.
Submitted (NOV-2000) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"A gene from tumor."
Mai N., Liu C.
Submitted (NOV-2000) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Lung.
[5]"A set of consensus mammalian mediator subunits identified by multidimensional protein identification technology."
Sato S., Tomomori-Sato C., Parmely T.J., Florens L., Zybailov B., Swanson S.K., Banks C.A.S., Jin J., Cai Y., Washburn M.P., Conaway J.W., Conaway R.C.
Mol. Cell 14:685-691(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE MEDIATOR COMPLEX.
[6]"Magicin, a novel cytoskeletal protein associates with the NF2 tumor suppressor merlin and Grb2."
Wiederhold T., Lee M.-F., James M., Neujahr R., Smith N., Murthy A., Hartwig J., Gusella J.F., Ramesh V.
Oncogene 23:8815-8825(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION IN SIGNALING, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INTERACTION WITH ACTIN; NF2 AND GRB2.
[7]"MED1/TRAP220 exists predominantly in a TRAP/Mediator subpopulation enriched in RNA polymerase II and is required for ER-mediated transcription."
Zhang X., Krutchinsky A., Fukuda A., Chen W., Yamamura S., Chait B.T., Roeder R.G.
Mol. Cell 19:89-100(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH CCNC; MED1; MED6; MED12; MED13; MED16; MED17; MED20; MED21 AND MED24, IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE MEDIATOR COMPLEX, ASSOCIATION OF THE MEDIATOR COMPLEX WITH RNA POLYMERASE II.
[8]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF321617 mRNA. Translation: AAG38612.1.
AF358829 mRNA. Translation: AAK32724.1.
AF318059 mRNA. Translation: AAL26906.1.
AF317680 mRNA. Translation: AAK11563.1. Frameshift.
BC011936 mRNA. Translation: AAH11936.1.
RefSeqNP_079481.2. NM_025205.3.
UniGeneHs.731966.

3D structure databases

ProteinModelPortalQ9H204.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid123219. 75 interactions.
IntActQ9H204. 47 interactions.
MINTMINT-1434492.
STRING9606.ENSP00000237380.

PTM databases

PhosphoSiteQ9H204.

Polymorphism databases

DMDM59799838.

Proteomic databases

PaxDbQ9H204.
PRIDEQ9H204.

Protocols and materials databases

DNASU80306.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000237380; ENSP00000237380; ENSG00000118579.
GeneID80306.
KEGGhsa:80306.
UCSCuc003gpi.1. human.

Organism-specific databases

CTD80306.
GeneCardsGC04P017616.
HGNCHGNC:24628. MED28.
HPAHPA035900.
HPA035901.
MIM610311. gene.
neXtProtNX_Q9H204.
PharmGKBPA134935853.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG78801.
HOGENOMHOG000113540.
HOVERGENHBG052448.
InParanoidQ9H204.
KOK15141.
OrthoDBEOG70ZZPZ.
PhylomeDBQ9H204.
TreeFamTF326988.

Gene expression databases

ArrayExpressQ9H204.
BgeeQ9H204.
CleanExHS_MED28.
GenevestigatorQ9H204.

Family and domain databases

InterProIPR021640. Mediator_Med28.
[Graphical view]
PfamPF11594. Med28. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSMED28. human.
GeneWikiMED28.
GenomeRNAi80306.
NextBio70776.
PROQ9H204.
SOURCESearch...

Entry information

Entry nameMED28_HUMAN
AccessionPrimary (citable) accession number: Q9H204
Secondary accession number(s): Q9BZJ5
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 2005
Last sequence update: March 1, 2001
Last modified: April 16, 2014
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 4

Human chromosome 4: entries, gene names and cross-references to MIM