Q9H204 (MED28_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Mediator of RNA polymerase II transcription subunit 28

Alternative name(s):
Endothelial-derived protein 1
Mediator complex subunit 28
Merlin and Grb2-interacting cytoskeletal protein
Short name=Magicin
Tumor angiogenesis marker EG-1

Gene names

Name:	MED28
Synonyms:	EG1
ORF Names:	FKSG20

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	178 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. May be part of a complex containing NF2/merlin that participates in cellular signaling to the actin cytoskeleton downstream of tyrosine kinase signaling pathways. Ref.6
Subunit structure	Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP. Forms a ternary complex with NF2/merlin and GRB2. Binds to actin. Ref.5 Ref.6 Ref.7
Subcellular location	Nucleus. Cytoplasm. Membrane; Peripheral membrane protein. Note: According to Ref.6, it is cytoplasmic and mainly membrane-associated. Ref.6
Tissue specificity	Widely expressed. Highly expressed in vascular tissues such as placenta, testis and liver. Ref.6
Induction	Up-regulated by endothelial cells when exposed to tumor conditional media. Ref.1
Sequence similarities	Belongs to the Mediator complex subunit 28 family.
Sequence caution	The sequence AAK11563.1 differs from that shown. Reason: Frameshift at position 165.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Cytoplasm Membrane Nucleus
Domain	Coiled coil
Ligand	Actin-binding
Molecular function	Activator
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	negative regulation of smooth muscle cell differentiation Inferred from electronic annotation. Source: Ensembl regulation of transcription, DNA-templated Inferred from electronic annotation. Source: UniProtKB-KW stem cell maintenance Inferred from electronic annotation. Source: Ensembl transcription, DNA-templated Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cortical actin cytoskeleton Inferred from electronic annotation. Source: Ensembl mediator complex Inferred from electronic annotation. Source: Ensembl membrane Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from direct assay. Source: HPA
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
	P03255	2	EBI-514199,EBI-2603114	From a different organism.
FYN	P06241	6	EBI-514199,EBI-515315
GRB2	P62993	3	EBI-514199,EBI-401755
LCK	P06239	4	EBI-514199,EBI-1348
MAGI3	Q5TCQ9	2	EBI-514199,EBI-310506
NF2	P35240	4	EBI-514199,EBI-1014472
NF2	P35240-1	2	EBI-514199,EBI-1014500
PRMT6	Q96LA8	2	EBI-514199,EBI-912440
SRC	P12931	3	EBI-514199,EBI-621482
YES1	P07947	2	EBI-514199,EBI-515331

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 178	178	Mediator of RNA polymerase II transcription subunit 28		PRO_0000113981
Regions
	Coiled coil	109 – 145	37	Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9H204 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: 63DE023972908066	FASTA	178	19,520
	10 20 30 40 50 60 MAAPLGGMFS GQPPGPPQAP PGLPGQASLL QAAPGAPRPS SSTLVDELES SFEACFASLV 70 80 90 100 110 120 SQDYVNGTDQ EEIRTGVDQC IQKFLDIARQ TECFFLQKRL QLSVQKPEQV IKEDVSELRN 130 140 150 160 170 ELQRKDALVQ KHLTKLRHWQ QVLEDINVQH KKPADIPQGS LAYLEQASAN IPAPLKPT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of a novel endothelial-derived gene EG-1." Liu C., Zhang L., Shao Z.-M., Beatty P., Sartippour M., Lane T.F., Barsky S.H., Livingston E., Nguyen M.H. Biochem. Biophys. Res. Commun. 290:602-612(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], INDUCTION.
[2]	"Cloning of FKSG20, a novel gene expressed in adrenal gland tumor tissues." Wang Y.-G., Gong L. Submitted (NOV-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"A gene from tumor." Mai N., Liu C. Submitted (NOV-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Lung.
[5]	"A set of consensus mammalian mediator subunits identified by multidimensional protein identification technology." Sato S., Tomomori-Sato C., Parmely T.J., Florens L., Zybailov B., Swanson S.K., Banks C.A.S., Jin J., Cai Y., Washburn M.P., Conaway J.W., Conaway R.C. Mol. Cell 14:685-691(2004) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE MEDIATOR COMPLEX.
[6]	"Magicin, a novel cytoskeletal protein associates with the NF2 tumor suppressor merlin and Grb2." Wiederhold T., Lee M.-F., James M., Neujahr R., Smith N., Murthy A., Hartwig J., Gusella J.F., Ramesh V. Oncogene 23:8815-8825(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION IN SIGNALING, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INTERACTION WITH ACTIN; NF2 AND GRB2.
[7]	"MED1/TRAP220 exists predominantly in a TRAP/Mediator subpopulation enriched in RNA polymerase II and is required for ER-mediated transcription." Zhang X., Krutchinsky A., Fukuda A., Chen W., Yamamura S., Chait B.T., Roeder R.G. Mol. Cell 19:89-100(2005) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH CCNC; MED1; MED6; MED12; MED13; MED16; MED17; MED20; MED21 AND MED24, IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE MEDIATOR COMPLEX, ASSOCIATION OF THE MEDIATOR COMPLEX WITH RNA POLYMERASE II.
[8]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Web resources

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF321617 mRNA. Translation: AAG38612.1. AF358829 mRNA. Translation: AAK32724.1. AF318059 mRNA. Translation: AAL26906.1. AF317680 mRNA. Translation: AAK11563.1. Frameshift. BC011936 mRNA. Translation: AAH11936.1.
RefSeq	NP_079481.2. NM_025205.3.
UniGene	Hs.731966.
3D structure databases
ProteinModelPortal	Q9H204.
ModBase	Search...
MobiDB	Search...
Protein-protein interaction databases
BioGrid	123219. 75 interactions.
IntAct	Q9H204. 47 interactions.
MINT	MINT-1434492.
STRING	9606.ENSP00000237380.
PTM databases
PhosphoSite	Q9H204.
Polymorphism databases
DMDM	59799838.
Proteomic databases
PaxDb	Q9H204.
PRIDE	Q9H204.
Protocols and materials databases
DNASU	80306.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000237380; ENSP00000237380; ENSG00000118579.
GeneID	80306.
KEGG	hsa:80306.
UCSC	uc003gpi.1. human.
Organism-specific databases
CTD	80306.
GeneCards	GC04P017616.
HGNC	HGNC:24628. MED28.
HPA	HPA035900. HPA035901.
MIM	610311. gene.
neXtProt	NX_Q9H204.
PharmGKB	PA134935853.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG78801.
HOGENOM	HOG000113540.
HOVERGEN	HBG052448.
InParanoid	Q9H204.
KO	K15141.
OrthoDB	EOG70ZZPZ.
TreeFam	TF326988.
Gene expression databases
ArrayExpress	Q9H204.
Bgee	Q9H204.
CleanEx	HS_MED28.
Genevestigator	Q9H204.
Family and domain databases
InterPro	IPR021640. Mediator_Med28. [Graphical view]
Pfam	PF11594. Med28. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	MED28. human.
GeneWiki	MED28.
GenomeRNAi	80306.
NextBio	70776.
PRO	Q9H204.
SOURCE	Search...

Entry information

Entry name

MED28_HUMAN

Accession

Primary (citable) accession number: Q9H204
Secondary accession number(s): Q9BZJ5

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 2005
Last sequence update:	March 1, 2001
Last modified:	February 19, 2014
This is version 107 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.