Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q9H204

- MED28_HUMAN

UniProt

Q9H204 - MED28_HUMAN

Protein

Mediator of RNA polymerase II transcription subunit 28

Gene

MED28

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 112 (01 Oct 2014)
      Sequence version 1 (01 Mar 2001)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. May be part of a complex containing NF2/merlin that participates in cellular signaling to the actin cytoskeleton downstream of tyrosine kinase signaling pathways.1 Publication

    GO - Molecular functioni

    1. protein binding Source: IntAct

    GO - Biological processi

    1. negative regulation of smooth muscle cell differentiation Source: Ensembl
    2. regulation of transcription, DNA-templated Source: UniProtKB-KW
    3. stem cell maintenance Source: Ensembl
    4. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Activator

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    Actin-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Mediator of RNA polymerase II transcription subunit 28
    Alternative name(s):
    Endothelial-derived protein 1
    Mediator complex subunit 28
    Merlin and Grb2-interacting cytoskeletal protein
    Short name:
    Magicin
    Tumor angiogenesis marker EG-1
    Gene namesi
    Name:MED28
    Synonyms:EG1
    ORF Names:FKSG20
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 4

    Organism-specific databases

    HGNCiHGNC:24628. MED28.

    Subcellular locationi

    Nucleus 1 Publication. Cytoplasm 1 Publication. Membrane 1 Publication; Peripheral membrane protein 1 Publication
    Note: According to PubMed:15467741, it is cytoplasmic and mainly membrane-associated.

    GO - Cellular componenti

    1. cortical actin cytoskeleton Source: Ensembl
    2. mediator complex Source: Ensembl
    3. membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm, Membrane, Nucleus

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA134935853.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 178178Mediator of RNA polymerase II transcription subunit 28PRO_0000113981Add
    BLAST

    Proteomic databases

    MaxQBiQ9H204.
    PaxDbiQ9H204.
    PRIDEiQ9H204.

    PTM databases

    PhosphoSiteiQ9H204.

    Expressioni

    Tissue specificityi

    Widely expressed. Highly expressed in vascular tissues such as placenta, testis and liver.1 Publication

    Inductioni

    Up-regulated by endothelial cells when exposed to tumor conditional media.1 Publication

    Gene expression databases

    ArrayExpressiQ9H204.
    BgeeiQ9H204.
    CleanExiHS_MED28.
    GenevestigatoriQ9H204.

    Organism-specific databases

    HPAiHPA035900.
    HPA035901.

    Interactioni

    Subunit structurei

    Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP. Forms a ternary complex with NF2/merlin and GRB2. Binds to actin.2 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    P032552EBI-514199,EBI-2603114From a different organism.
    FYNP062416EBI-514199,EBI-515315
    GRB2P629933EBI-514199,EBI-401755
    LCKP062394EBI-514199,EBI-1348
    MAGI3Q5TCQ92EBI-514199,EBI-310506
    NF2P352404EBI-514199,EBI-1014472
    NF2P35240-12EBI-514199,EBI-1014500
    PRMT6Q96LA82EBI-514199,EBI-912440
    SRCP129313EBI-514199,EBI-621482
    YES1P079472EBI-514199,EBI-515331

    Protein-protein interaction databases

    BioGridi123219. 75 interactions.
    IntActiQ9H204. 47 interactions.
    MINTiMINT-1434492.
    STRINGi9606.ENSP00000237380.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9H204.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili109 – 14537Sequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Belongs to the Mediator complex subunit 28 family.Curated

    Keywords - Domaini

    Coiled coil

    Phylogenomic databases

    eggNOGiNOG78801.
    HOGENOMiHOG000113540.
    HOVERGENiHBG052448.
    InParanoidiQ9H204.
    KOiK15141.
    OrthoDBiEOG70ZZPZ.
    PhylomeDBiQ9H204.
    TreeFamiTF326988.

    Family and domain databases

    InterProiIPR021640. Mediator_Med28.
    [Graphical view]
    PfamiPF11594. Med28. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9H204-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAAPLGGMFS GQPPGPPQAP PGLPGQASLL QAAPGAPRPS SSTLVDELES    50
    SFEACFASLV SQDYVNGTDQ EEIRTGVDQC IQKFLDIARQ TECFFLQKRL 100
    QLSVQKPEQV IKEDVSELRN ELQRKDALVQ KHLTKLRHWQ QVLEDINVQH 150
    KKPADIPQGS LAYLEQASAN IPAPLKPT 178
    Length:178
    Mass (Da):19,520
    Last modified:March 1, 2001 - v1
    Checksum:i63DE023972908066
    GO

    Sequence cautioni

    The sequence AAK11563.1 differs from that shown. Reason: Frameshift at position 165.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF321617 mRNA. Translation: AAG38612.1.
    AF358829 mRNA. Translation: AAK32724.1.
    AF318059 mRNA. Translation: AAL26906.1.
    AF317680 mRNA. Translation: AAK11563.1. Frameshift.
    BC011936 mRNA. Translation: AAH11936.1.
    CCDSiCCDS33963.1.
    RefSeqiNP_079481.2. NM_025205.3.
    UniGeneiHs.731966.

    Genome annotation databases

    EnsembliENST00000237380; ENSP00000237380; ENSG00000118579.
    GeneIDi80306.
    KEGGihsa:80306.
    UCSCiuc003gpi.1. human.

    Polymorphism databases

    DMDMi59799838.

    Cross-referencesi

    Web resourcesi

    Atlas of Genetics and Cytogenetics in Oncology and Haematology

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF321617 mRNA. Translation: AAG38612.1 .
    AF358829 mRNA. Translation: AAK32724.1 .
    AF318059 mRNA. Translation: AAL26906.1 .
    AF317680 mRNA. Translation: AAK11563.1 . Frameshift.
    BC011936 mRNA. Translation: AAH11936.1 .
    CCDSi CCDS33963.1.
    RefSeqi NP_079481.2. NM_025205.3.
    UniGenei Hs.731966.

    3D structure databases

    ProteinModelPortali Q9H204.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 123219. 75 interactions.
    IntActi Q9H204. 47 interactions.
    MINTi MINT-1434492.
    STRINGi 9606.ENSP00000237380.

    PTM databases

    PhosphoSitei Q9H204.

    Polymorphism databases

    DMDMi 59799838.

    Proteomic databases

    MaxQBi Q9H204.
    PaxDbi Q9H204.
    PRIDEi Q9H204.

    Protocols and materials databases

    DNASUi 80306.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000237380 ; ENSP00000237380 ; ENSG00000118579 .
    GeneIDi 80306.
    KEGGi hsa:80306.
    UCSCi uc003gpi.1. human.

    Organism-specific databases

    CTDi 80306.
    GeneCardsi GC04P017616.
    HGNCi HGNC:24628. MED28.
    HPAi HPA035900.
    HPA035901.
    MIMi 610311. gene.
    neXtProti NX_Q9H204.
    PharmGKBi PA134935853.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG78801.
    HOGENOMi HOG000113540.
    HOVERGENi HBG052448.
    InParanoidi Q9H204.
    KOi K15141.
    OrthoDBi EOG70ZZPZ.
    PhylomeDBi Q9H204.
    TreeFami TF326988.

    Miscellaneous databases

    ChiTaRSi MED28. human.
    GeneWikii MED28.
    GenomeRNAii 80306.
    NextBioi 70776.
    PROi Q9H204.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q9H204.
    Bgeei Q9H204.
    CleanExi HS_MED28.
    Genevestigatori Q9H204.

    Family and domain databases

    InterProi IPR021640. Mediator_Med28.
    [Graphical view ]
    Pfami PF11594. Med28. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [MRNA], INDUCTION.
    2. "Cloning of FKSG20, a novel gene expressed in adrenal gland tumor tissues."
      Wang Y.-G., Gong L.
      Submitted (NOV-2000) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    3. "A gene from tumor."
      Mai N., Liu C.
      Submitted (NOV-2000) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Lung.
    5. "A set of consensus mammalian mediator subunits identified by multidimensional protein identification technology."
      Sato S., Tomomori-Sato C., Parmely T.J., Florens L., Zybailov B., Swanson S.K., Banks C.A.S., Jin J., Cai Y., Washburn M.P., Conaway J.W., Conaway R.C.
      Mol. Cell 14:685-691(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE MEDIATOR COMPLEX.
    6. "Magicin, a novel cytoskeletal protein associates with the NF2 tumor suppressor merlin and Grb2."
      Wiederhold T., Lee M.-F., James M., Neujahr R., Smith N., Murthy A., Hartwig J., Gusella J.F., Ramesh V.
      Oncogene 23:8815-8825(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION IN SIGNALING, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INTERACTION WITH ACTIN; NF2 AND GRB2.
    7. "MED1/TRAP220 exists predominantly in a TRAP/Mediator subpopulation enriched in RNA polymerase II and is required for ER-mediated transcription."
      Zhang X., Krutchinsky A., Fukuda A., Chen W., Yamamura S., Chait B.T., Roeder R.G.
      Mol. Cell 19:89-100(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH CCNC; MED1; MED6; MED12; MED13; MED16; MED17; MED20; MED21 AND MED24, IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE MEDIATOR COMPLEX, ASSOCIATION OF THE MEDIATOR COMPLEX WITH RNA POLYMERASE II.
    8. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiMED28_HUMAN
    AccessioniPrimary (citable) accession number: Q9H204
    Secondary accession number(s): Q9BZJ5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 2005
    Last sequence update: March 1, 2001
    Last modified: October 1, 2014
    This is version 112 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 4
      Human chromosome 4: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3