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Q9H204

- MED28_HUMAN

UniProt

Q9H204 - MED28_HUMAN

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Protein
Mediator of RNA polymerase II transcription subunit 28
Gene
MED28, EG1, FKSG20
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. May be part of a complex containing NF2/merlin that participates in cellular signaling to the actin cytoskeleton downstream of tyrosine kinase signaling pathways.1 Publication

GO - Molecular functioni

  1. protein binding Source: IntAct

GO - Biological processi

  1. negative regulation of smooth muscle cell differentiation Source: Ensembl
  2. regulation of transcription, DNA-templated Source: UniProtKB-KW
  3. stem cell maintenance Source: Ensembl
  4. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

Actin-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Mediator of RNA polymerase II transcription subunit 28
Alternative name(s):
Endothelial-derived protein 1
Mediator complex subunit 28
Merlin and Grb2-interacting cytoskeletal protein
Short name:
Magicin
Tumor angiogenesis marker EG-1
Gene namesi
Name:MED28
Synonyms:EG1
ORF Names:FKSG20
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 4

Organism-specific databases

HGNCiHGNC:24628. MED28.

Subcellular locationi

Nucleus. Cytoplasm. Membrane; Peripheral membrane protein
Note: According to 1 Publication, it is cytoplasmic and mainly membrane-associated.1 Publication

GO - Cellular componenti

  1. cortical actin cytoskeleton Source: Ensembl
  2. mediator complex Source: Ensembl
  3. membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane, Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134935853.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 178178Mediator of RNA polymerase II transcription subunit 28
PRO_0000113981Add
BLAST

Proteomic databases

MaxQBiQ9H204.
PaxDbiQ9H204.
PRIDEiQ9H204.

PTM databases

PhosphoSiteiQ9H204.

Expressioni

Tissue specificityi

Widely expressed. Highly expressed in vascular tissues such as placenta, testis and liver.1 Publication

Inductioni

Up-regulated by endothelial cells when exposed to tumor conditional media.1 Publication

Gene expression databases

ArrayExpressiQ9H204.
BgeeiQ9H204.
CleanExiHS_MED28.
GenevestigatoriQ9H204.

Organism-specific databases

HPAiHPA035900.
HPA035901.

Interactioni

Subunit structurei

Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP. Forms a ternary complex with NF2/merlin and GRB2. Binds to actin.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
P032552EBI-514199,EBI-2603114From a different organism.
FYNP062416EBI-514199,EBI-515315
GRB2P629933EBI-514199,EBI-401755
LCKP062394EBI-514199,EBI-1348
MAGI3Q5TCQ92EBI-514199,EBI-310506
NF2P352404EBI-514199,EBI-1014472
NF2P35240-12EBI-514199,EBI-1014500
PRMT6Q96LA82EBI-514199,EBI-912440
SRCP129313EBI-514199,EBI-621482
YES1P079472EBI-514199,EBI-515331

Protein-protein interaction databases

BioGridi123219. 75 interactions.
IntActiQ9H204. 47 interactions.
MINTiMINT-1434492.
STRINGi9606.ENSP00000237380.

Structurei

3D structure databases

ProteinModelPortaliQ9H204.

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili109 – 14537 Reviewed prediction
Add
BLAST

Sequence similaritiesi

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiNOG78801.
HOGENOMiHOG000113540.
HOVERGENiHBG052448.
InParanoidiQ9H204.
KOiK15141.
OrthoDBiEOG70ZZPZ.
PhylomeDBiQ9H204.
TreeFamiTF326988.

Family and domain databases

InterProiIPR021640. Mediator_Med28.
[Graphical view]
PfamiPF11594. Med28. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9H204-1 [UniParc]FASTAAdd to Basket

« Hide

MAAPLGGMFS GQPPGPPQAP PGLPGQASLL QAAPGAPRPS SSTLVDELES    50
SFEACFASLV SQDYVNGTDQ EEIRTGVDQC IQKFLDIARQ TECFFLQKRL 100
QLSVQKPEQV IKEDVSELRN ELQRKDALVQ KHLTKLRHWQ QVLEDINVQH 150
KKPADIPQGS LAYLEQASAN IPAPLKPT 178
Length:178
Mass (Da):19,520
Last modified:March 1, 2001 - v1
Checksum:i63DE023972908066
GO

Sequence cautioni

The sequence AAK11563.1 differs from that shown. Reason: Frameshift at position 165.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF321617 mRNA. Translation: AAG38612.1.
AF358829 mRNA. Translation: AAK32724.1.
AF318059 mRNA. Translation: AAL26906.1.
AF317680 mRNA. Translation: AAK11563.1. Frameshift.
BC011936 mRNA. Translation: AAH11936.1.
CCDSiCCDS33963.1.
RefSeqiNP_079481.2. NM_025205.3.
UniGeneiHs.731966.

Genome annotation databases

EnsembliENST00000237380; ENSP00000237380; ENSG00000118579.
GeneIDi80306.
KEGGihsa:80306.
UCSCiuc003gpi.1. human.

Polymorphism databases

DMDMi59799838.

Cross-referencesi

Web resourcesi

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF321617 mRNA. Translation: AAG38612.1 .
AF358829 mRNA. Translation: AAK32724.1 .
AF318059 mRNA. Translation: AAL26906.1 .
AF317680 mRNA. Translation: AAK11563.1 . Frameshift.
BC011936 mRNA. Translation: AAH11936.1 .
CCDSi CCDS33963.1.
RefSeqi NP_079481.2. NM_025205.3.
UniGenei Hs.731966.

3D structure databases

ProteinModelPortali Q9H204.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 123219. 75 interactions.
IntActi Q9H204. 47 interactions.
MINTi MINT-1434492.
STRINGi 9606.ENSP00000237380.

PTM databases

PhosphoSitei Q9H204.

Polymorphism databases

DMDMi 59799838.

Proteomic databases

MaxQBi Q9H204.
PaxDbi Q9H204.
PRIDEi Q9H204.

Protocols and materials databases

DNASUi 80306.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000237380 ; ENSP00000237380 ; ENSG00000118579 .
GeneIDi 80306.
KEGGi hsa:80306.
UCSCi uc003gpi.1. human.

Organism-specific databases

CTDi 80306.
GeneCardsi GC04P017616.
HGNCi HGNC:24628. MED28.
HPAi HPA035900.
HPA035901.
MIMi 610311. gene.
neXtProti NX_Q9H204.
PharmGKBi PA134935853.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG78801.
HOGENOMi HOG000113540.
HOVERGENi HBG052448.
InParanoidi Q9H204.
KOi K15141.
OrthoDBi EOG70ZZPZ.
PhylomeDBi Q9H204.
TreeFami TF326988.

Miscellaneous databases

ChiTaRSi MED28. human.
GeneWikii MED28.
GenomeRNAii 80306.
NextBioi 70776.
PROi Q9H204.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q9H204.
Bgeei Q9H204.
CleanExi HS_MED28.
Genevestigatori Q9H204.

Family and domain databases

InterProi IPR021640. Mediator_Med28.
[Graphical view ]
Pfami PF11594. Med28. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA], INDUCTION.
  2. "Cloning of FKSG20, a novel gene expressed in adrenal gland tumor tissues."
    Wang Y.-G., Gong L.
    Submitted (NOV-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "A gene from tumor."
    Mai N., Liu C.
    Submitted (NOV-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Lung.
  5. "A set of consensus mammalian mediator subunits identified by multidimensional protein identification technology."
    Sato S., Tomomori-Sato C., Parmely T.J., Florens L., Zybailov B., Swanson S.K., Banks C.A.S., Jin J., Cai Y., Washburn M.P., Conaway J.W., Conaway R.C.
    Mol. Cell 14:685-691(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE MEDIATOR COMPLEX.
  6. "Magicin, a novel cytoskeletal protein associates with the NF2 tumor suppressor merlin and Grb2."
    Wiederhold T., Lee M.-F., James M., Neujahr R., Smith N., Murthy A., Hartwig J., Gusella J.F., Ramesh V.
    Oncogene 23:8815-8825(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN SIGNALING, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INTERACTION WITH ACTIN; NF2 AND GRB2.
  7. "MED1/TRAP220 exists predominantly in a TRAP/Mediator subpopulation enriched in RNA polymerase II and is required for ER-mediated transcription."
    Zhang X., Krutchinsky A., Fukuda A., Chen W., Yamamura S., Chait B.T., Roeder R.G.
    Mol. Cell 19:89-100(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH CCNC; MED1; MED6; MED12; MED13; MED16; MED17; MED20; MED21 AND MED24, IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE MEDIATOR COMPLEX, ASSOCIATION OF THE MEDIATOR COMPLEX WITH RNA POLYMERASE II.
  8. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiMED28_HUMAN
AccessioniPrimary (citable) accession number: Q9H204
Secondary accession number(s): Q9BZJ5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 2005
Last sequence update: March 1, 2001
Last modified: July 9, 2014
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 4
    Human chromosome 4: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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