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Protein

Mediator of RNA polymerase II transcription subunit 28

Gene

MED28

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. May be part of a complex containing NF2/merlin that participates in cellular signaling to the actin cytoskeleton downstream of tyrosine kinase signaling pathways.1 Publication

GO - Biological processi

  1. negative regulation of smooth muscle cell differentiation Source: Ensembl
  2. regulation of transcription, DNA-templated Source: UniProtKB-KW
  3. stem cell maintenance Source: Ensembl
  4. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

Actin-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Mediator of RNA polymerase II transcription subunit 28
Alternative name(s):
Endothelial-derived protein 1
Mediator complex subunit 28
Merlin and Grb2-interacting cytoskeletal protein
Short name:
Magicin
Tumor angiogenesis marker EG-1
Gene namesi
Name:MED28
Synonyms:EG1
ORF Names:FKSG20
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 4

Organism-specific databases

HGNCiHGNC:24628. MED28.

Subcellular locationi

  1. Nucleus 1 Publication
  2. Cytoplasm 1 Publication
  3. Membrane 1 Publication; Peripheral membrane protein 1 Publication

  4. Note: According to PubMed:15467741, it is cytoplasmic and mainly membrane-associated.

GO - Cellular componenti

  1. cortical actin cytoskeleton Source: Ensembl
  2. mediator complex Source: Ensembl
  3. membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane, Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134935853.

Polymorphism and mutation databases

BioMutaiMED28.
DMDMi59799838.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 178178Mediator of RNA polymerase II transcription subunit 28PRO_0000113981Add
BLAST

Proteomic databases

MaxQBiQ9H204.
PaxDbiQ9H204.
PRIDEiQ9H204.

PTM databases

PhosphoSiteiQ9H204.

Expressioni

Tissue specificityi

Widely expressed. Highly expressed in vascular tissues such as placenta, testis and liver.1 Publication

Inductioni

Up-regulated by endothelial cells when exposed to tumor conditional media.1 Publication

Gene expression databases

BgeeiQ9H204.
CleanExiHS_MED28.
ExpressionAtlasiQ9H204. baseline and differential.
GenevestigatoriQ9H204.

Organism-specific databases

HPAiHPA035900.
HPA035901.

Interactioni

Subunit structurei

Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP. Forms a ternary complex with NF2/merlin and GRB2. Binds to actin.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
P032552EBI-514199,EBI-2603114From a different organism.
FYNP062416EBI-514199,EBI-515315
GRB2P629933EBI-514199,EBI-401755
LCKP062394EBI-514199,EBI-1348
MAGI3Q5TCQ92EBI-514199,EBI-310506
Med11Q9D8C62EBI-514199,EBI-6260909From a different organism.
MED22Q155282EBI-514199,EBI-394687
MED29Q9NX703EBI-514199,EBI-394656
Med30Q9CQI92EBI-514199,EBI-309220From a different organism.
Med8Q9D7W52EBI-514199,EBI-7990252From a different organism.
NF2P352404EBI-514199,EBI-1014472
NF2P35240-12EBI-514199,EBI-1014500
PRMT6Q96LA82EBI-514199,EBI-912440
PUF60Q9UHX16EBI-514199,EBI-1053259
SPERTQ8NA613EBI-514199,EBI-741724
SRCP129313EBI-514199,EBI-621482
YES1P079472EBI-514199,EBI-515331

Protein-protein interaction databases

BioGridi123219. 79 interactions.
IntActiQ9H204. 56 interactions.
MINTiMINT-1434492.
STRINGi9606.ENSP00000237380.

Structurei

3D structure databases

ProteinModelPortaliQ9H204.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili109 – 14537Sequence AnalysisAdd
BLAST

Sequence similaritiesi

Belongs to the Mediator complex subunit 28 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiNOG78801.
GeneTreeiENSGT00390000006192.
HOGENOMiHOG000113540.
HOVERGENiHBG052448.
InParanoidiQ9H204.
KOiK15141.
OrthoDBiEOG70ZZPZ.
PhylomeDBiQ9H204.
TreeFamiTF326988.

Family and domain databases

InterProiIPR021640. Mediator_Med28.
[Graphical view]
PfamiPF11594. Med28. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9H204-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAPLGGMFS GQPPGPPQAP PGLPGQASLL QAAPGAPRPS SSTLVDELES
60 70 80 90 100
SFEACFASLV SQDYVNGTDQ EEIRTGVDQC IQKFLDIARQ TECFFLQKRL
110 120 130 140 150
QLSVQKPEQV IKEDVSELRN ELQRKDALVQ KHLTKLRHWQ QVLEDINVQH
160 170
KKPADIPQGS LAYLEQASAN IPAPLKPT
Length:178
Mass (Da):19,520
Last modified:March 1, 2001 - v1
Checksum:i63DE023972908066
GO

Sequence cautioni

The sequence AAK11563.1 differs from that shown. Reason: Frameshift at position 165. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF321617 mRNA. Translation: AAG38612.1.
AF358829 mRNA. Translation: AAK32724.1.
AF318059 mRNA. Translation: AAL26906.1.
AF317680 mRNA. Translation: AAK11563.1. Frameshift.
BC011936 mRNA. Translation: AAH11936.1.
CCDSiCCDS33963.1.
RefSeqiNP_079481.2. NM_025205.4.
UniGeneiHs.731966.

Genome annotation databases

EnsembliENST00000237380; ENSP00000237380; ENSG00000118579.
GeneIDi80306.
KEGGihsa:80306.
UCSCiuc003gpi.1. human.

Polymorphism and mutation databases

BioMutaiMED28.

Cross-referencesi

Web resourcesi

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF321617 mRNA. Translation: AAG38612.1.
AF358829 mRNA. Translation: AAK32724.1.
AF318059 mRNA. Translation: AAL26906.1.
AF317680 mRNA. Translation: AAK11563.1. Frameshift.
BC011936 mRNA. Translation: AAH11936.1.
CCDSiCCDS33963.1.
RefSeqiNP_079481.2. NM_025205.4.
UniGeneiHs.731966.

3D structure databases

ProteinModelPortaliQ9H204.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi123219. 79 interactions.
IntActiQ9H204. 56 interactions.
MINTiMINT-1434492.
STRINGi9606.ENSP00000237380.

PTM databases

PhosphoSiteiQ9H204.

Polymorphism and mutation databases

BioMutaiMED28.
DMDMi59799838.

Proteomic databases

MaxQBiQ9H204.
PaxDbiQ9H204.
PRIDEiQ9H204.

Protocols and materials databases

DNASUi80306.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000237380; ENSP00000237380; ENSG00000118579.
GeneIDi80306.
KEGGihsa:80306.
UCSCiuc003gpi.1. human.

Organism-specific databases

CTDi80306.
GeneCardsiGC04P017616.
HGNCiHGNC:24628. MED28.
HPAiHPA035900.
HPA035901.
MIMi610311. gene.
neXtProtiNX_Q9H204.
PharmGKBiPA134935853.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG78801.
GeneTreeiENSGT00390000006192.
HOGENOMiHOG000113540.
HOVERGENiHBG052448.
InParanoidiQ9H204.
KOiK15141.
OrthoDBiEOG70ZZPZ.
PhylomeDBiQ9H204.
TreeFamiTF326988.

Miscellaneous databases

ChiTaRSiMED28. human.
GeneWikiiMED28.
GenomeRNAii80306.
NextBioi70776.
PROiQ9H204.
SOURCEiSearch...

Gene expression databases

BgeeiQ9H204.
CleanExiHS_MED28.
ExpressionAtlasiQ9H204. baseline and differential.
GenevestigatoriQ9H204.

Family and domain databases

InterProiIPR021640. Mediator_Med28.
[Graphical view]
PfamiPF11594. Med28. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA], INDUCTION.
  2. "Cloning of FKSG20, a novel gene expressed in adrenal gland tumor tissues."
    Wang Y.-G., Gong L.
    Submitted (NOV-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "A gene from tumor."
    Mai N., Liu C.
    Submitted (NOV-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Lung.
  5. "A set of consensus mammalian mediator subunits identified by multidimensional protein identification technology."
    Sato S., Tomomori-Sato C., Parmely T.J., Florens L., Zybailov B., Swanson S.K., Banks C.A.S., Jin J., Cai Y., Washburn M.P., Conaway J.W., Conaway R.C.
    Mol. Cell 14:685-691(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE MEDIATOR COMPLEX.
  6. "Magicin, a novel cytoskeletal protein associates with the NF2 tumor suppressor merlin and Grb2."
    Wiederhold T., Lee M.-F., James M., Neujahr R., Smith N., Murthy A., Hartwig J., Gusella J.F., Ramesh V.
    Oncogene 23:8815-8825(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN SIGNALING, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INTERACTION WITH ACTIN; NF2 AND GRB2.
  7. "MED1/TRAP220 exists predominantly in a TRAP/Mediator subpopulation enriched in RNA polymerase II and is required for ER-mediated transcription."
    Zhang X., Krutchinsky A., Fukuda A., Chen W., Yamamura S., Chait B.T., Roeder R.G.
    Mol. Cell 19:89-100(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH CCNC; MED1; MED6; MED12; MED13; MED16; MED17; MED20; MED21 AND MED24, IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE MEDIATOR COMPLEX, ASSOCIATION OF THE MEDIATOR COMPLEX WITH RNA POLYMERASE II.
  8. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiMED28_HUMAN
AccessioniPrimary (citable) accession number: Q9H204
Secondary accession number(s): Q9BZJ5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 2005
Last sequence update: March 1, 2001
Last modified: April 29, 2015
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 4
    Human chromosome 4: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.