MED28

Functionⁱ

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. May be part of a complex containing NF2/merlin that participates in cellular signaling to the actin cytoskeleton downstream of tyrosine kinase signaling pathways.1 Publication

GO - Molecular functionⁱ

actin binding Source: UniProtKB-KW

Complete GO annotation...

GO - Biological processⁱ

negative regulation of smooth muscle cell differentiation Source: Ensembl
regulation of transcription, DNA-templated Source: UniProtKB-KW
stem cell population maintenance Source: Ensembl
transcription, DNA-templated Source: UniProtKB-KW

Complete GO annotation...

Keywordsⁱ

Molecular function	Actin-binding, Activator
Biological process	Transcription, Transcription regulation

Enzyme and pathway databases

Reactomeⁱ	R-HSA-1989781. PPARA activates gene expression. R-HSA-381340. Transcriptional regulation of white adipocyte differentiation.
SIGNORⁱ	Q9H204.

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Mediator of RNA polymerase II transcription subunit 28 Alternative name(s): Endothelial-derived protein 1 Mediator complex subunit 28 Merlin and Grb2-interacting cytoskeletal protein Short name: Magicin Tumor angiogenesis marker EG-1
Gene namesⁱ	Name:MED28 Synonyms:EG1 ORF Names:FKSG20
Organismⁱ	Homo sapiens (Human)
Taxonomic identifierⁱ	9606 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo
Proteomesⁱ	UP000005640 Componentⁱ: Chromosome 4

Organism-specific databases

Human Gene Nomenclature Database More...HGNCⁱ	HGNC:24628. MED28.

HGNCⁱ

HGNC:24628. MED28.

Subcellular locationⁱ

Note:

According to PubMed:15467741, it is cytoplasmic and mainly membrane-associated.

GO - Cellular componentⁱ

cortical actin cytoskeleton Source: Ensembl
mediator complex Source: GO_Central
membrane Source: UniProtKB-SubCell
nucleoplasm Source: HPA

Complete GO annotation...

Keywords - Cellular componentⁱ

Cytoplasm, Membrane, Nucleus

Pathology & Biotechⁱ

Organism-specific databases

DisGeNET More...DisGeNETⁱ	80306.
Open Targets More...OpenTargetsⁱ	ENSG00000118579.
PharmGKBⁱ	PA134935853.

Polymorphism and mutation databases

BioMutaⁱ	MED28.
DMDMⁱ	59799838.

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000113981	1 – 178	Mediator of RNA polymerase II transcription subunit 28Add BLAST		178

Proteomic databases

EPDⁱ	Q9H204.
MaxQB - The MaxQuant DataBase More...MaxQBⁱ	Q9H204.
PaxDbⁱ	Q9H204.
PeptideAtlas More...PeptideAtlasⁱ	Q9H204.
PRIDEⁱ	Q9H204.

PTM databases

iPTMnetⁱ	Q9H204.
PhosphoSitePlusⁱ	Q9H204.

Expressionⁱ

Tissue specificityⁱ

Widely expressed. Highly expressed in vascular tissues such as placenta, testis and liver.1 Publication

Inductionⁱ

Up-regulated by endothelial cells when exposed to tumor conditional media.1 Publication

Gene expression databases

Bgeeⁱ	ENSG00000118579.
CleanExⁱ	HS_MED28.
ExpressionAtlasⁱ	Q9H204. baseline and differential.
Genevisibleⁱ	Q9H204. HS.

Organism-specific databases

Human Protein Atlas More...HPAⁱ	HPA035900. HPA035901.

HPAⁱ

HPA035900.
HPA035901.

Interactionⁱ

Subunit structureⁱ

Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP. Forms a ternary complex with NF2/merlin and GRB2. Binds to actin.2 Publications

Binary interactionsⁱ

With	Entry	#Exp.	IntAct	Notes
	P03255	2	EBI-514199,EBI-2603114	From Human adenovirus C serotype 5.
FYN	P06241	6	EBI-514199,EBI-515315
GRB2	P62993	3	EBI-514199,EBI-401755
LCK	P06239	4	EBI-514199,EBI-1348
MAGI3	Q5TCQ9	2	EBI-514199,EBI-310506
Med11	Q9D8C6	2	EBI-514199,EBI-6260909	From Mus musculus.
MED22	Q15528	2	EBI-514199,EBI-394687
MED29	Q9NX70	3	EBI-514199,EBI-394656
MED30	Q96HR3	6	EBI-514199,EBI-394659
Med30	Q9CQI9	2	EBI-514199,EBI-309220	From Mus musculus.
Med8	Q9D7W5	2	EBI-514199,EBI-7990252	From Mus musculus.
NF2	P35240	4	EBI-514199,EBI-1014472
NF2	P35240-1	2	EBI-514199,EBI-1014500
PRMT6	Q96LA8	2	EBI-514199,EBI-912440
PUF60	Q9UHX1	6	EBI-514199,EBI-1053259
SPERT	Q8NA61	3	EBI-514199,EBI-741724
SRC	P12931	3	EBI-514199,EBI-621482
YES1	P07947	2	EBI-514199,EBI-515331

Show more details

GO - Molecular functionⁱ

actin binding Source: UniProtKB-KW

Complete GO annotation...

Protein-protein interaction databases

BioGridⁱ	123219. 86 interactors.
IntActⁱ	Q9H204. 57 interactors.
Molecular INTeraction database More...MINTⁱ	MINT-1434492.
STRINGⁱ	9606.ENSP00000237380.

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	Q9H204.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Coiled coil

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Coiled coilⁱ	109 – 145	Sequence analysisAdd BLAST		37

Sequence similaritiesⁱ

Belongs to the Mediator complex subunit 28 family.Curated

Keywords - Domainⁱ

Coiled coil

Phylogenomic databases

eggNOGⁱ	ENOG410IETV. Eukaryota. ENOG4111HUA. LUCA.
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00390000006192.
HOGENOMⁱ	HOG000113540.
HOVERGENⁱ	HBG052448.
InParanoidⁱ	Q9H204.
KEGG Orthology (KO) More...KOⁱ	K15141.
PhylomeDBⁱ	Q9H204.
TreeFamⁱ	TF326988.

Family and domain databases

InterProⁱ	View protein in InterPro IPR021640. Mediator_Med28.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF11594. Med28. 1 hit.

Sequenceⁱ

Sequence statusⁱ: Complete.

Q9H204-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MAAPLGGMFS GQPPGPPQAP PGLPGQASLL QAAPGAPRPS SSTLVDELES 
        60         70         80         90        100
SFEACFASLV SQDYVNGTDQ EEIRTGVDQC IQKFLDIARQ TECFFLQKRL 
       110        120        130        140        150
QLSVQKPEQV IKEDVSELRN ELQRKDALVQ KHLTKLRHWQ QVLEDINVQH 
       160        170 
KKPADIPQGS LAYLEQASAN IPAPLKPT

Length:178

Mass (Da):19,520

Last modified:March 1, 2001 - v1

Checksum:ⁱ63DE023972908066

GO

Sequence cautionⁱ

The sequence AAK11563 differs from that shown. Reason: Frameshift at position 165.Curated

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AF321617 mRNA. Translation: AAG38612.1. AF358829 mRNA. Translation: AAK32724.1. AF318059 mRNA. Translation: AAL26906.1. AF317680 mRNA. Translation: AAK11563.1. Frameshift. BC011936 mRNA. Translation: AAH11936.1.
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS33963.1.
NCBI Reference Sequences More...RefSeqⁱ	NP_079481.2. NM_025205.4.
UniGeneⁱ	Hs.731966.

Genome annotation databases

Ensemblⁱ	ENST00000237380; ENSP00000237380; ENSG00000118579.
GeneIDⁱ	80306.
KEGGⁱ	hsa:80306.
UCSC genome browser More...UCSCⁱ	uc003gpk.2. human.

Similar proteinsⁱ

Entry	Cluster members	Organisms	Length	Cluster ID	Cluster name	Size
Q9H204	K7BCC3 G3QFM0 H2PCZ5 G1S3S5	Homo sapiens (Human) Pan troglodytes (Chimpanzee) Gorilla gorilla gorilla (Western lowland gorilla) Pongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii) Nomascus leucogenys (Northern white-cheeked gibbon) (Hylobates leucogenys)	178	UniRef100_Q9H204	Cluster: Mediator of RNA polymerase II transcription subunit 28	5

Entry	Cluster members	Organisms	Length	Cluster ID	Cluster name	Size
Q9H204	K7BCC3 G3QFM0 H2PCZ5 G1S3S5 A0A096NZI2 UPI00027F31A1 UPI00083BF560 UPI0005333AD5 M3ZB42 UPI0005334BB1 +2	Homo sapiens (Human) Pan troglodytes (Chimpanzee) Gorilla gorilla gorilla (Western lowland gorilla) Pongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii) Nomascus leucogenys (Northern white-cheeked gibbon) (Hylobates leucogenys) Papio anubis (Olive baboon) Rhinopithecus bieti (Black snub-nosed monkey) (Pygathrix bieti) Rhinopithecus roxellana (Golden snub-nosed monkey) (Pygathrix roxellana)	178	UniRef90_Q9H204	Cluster: Mediator of RNA polymerase II transcription subunit 28	13

Entry	Cluster members	Organisms	Length	Cluster ID	Cluster name	Size
Q9H204	K7BCC3 G3QFM0 H2PCZ5 G1S3S5 A0A096NZI2 UPI00027F31A1 UPI00083BF560 M3ZB42 UPI0005333AD5 UPI0005334BB1 +23	Homo sapiens (Human) Pan troglodytes (Chimpanzee) Gorilla gorilla gorilla (Western lowland gorilla) Pongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii) Nomascus leucogenys (Northern white-cheeked gibbon) (Hylobates leucogenys) Papio anubis (Olive baboon) Rhinopithecus bieti (Black snub-nosed monkey) (Pygathrix bieti) Rhinopithecus roxellana (Golden snub-nosed monkey) (Pygathrix roxellana) Cuculus canorus (common cuckoo) Manacus vitellinus (golden-collared manakin) And more	178	UniRef50_Q9H204	Cluster: Mediator of RNA polymerase II transcription subunit 28	34

Cross-referencesⁱ

Web resourcesⁱ

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AF321617 mRNA. Translation: AAG38612.1. AF358829 mRNA. Translation: AAK32724.1. AF318059 mRNA. Translation: AAL26906.1. AF317680 mRNA. Translation: AAK11563.1. Frameshift. BC011936 mRNA. Translation: AAH11936.1.
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS33963.1.
NCBI Reference Sequences More...RefSeqⁱ	NP_079481.2. NM_025205.4.
UniGeneⁱ	Hs.731966.

3D structure databases

ProteinModelPortalⁱ	Q9H204.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	123219. 86 interactors.
IntActⁱ	Q9H204. 57 interactors.
Molecular INTeraction database More...MINTⁱ	MINT-1434492.
STRINGⁱ	9606.ENSP00000237380.

PTM databases

iPTMnetⁱ	Q9H204.
PhosphoSitePlusⁱ	Q9H204.

Polymorphism and mutation databases

BioMutaⁱ	MED28.
DMDMⁱ	59799838.

Proteomic databases

EPDⁱ	Q9H204.
MaxQB - The MaxQuant DataBase More...MaxQBⁱ	Q9H204.
PaxDbⁱ	Q9H204.
PeptideAtlas More...PeptideAtlasⁱ	Q9H204.
PRIDEⁱ	Q9H204.

Protocols and materials databases

The DNASU plasmid repository More...DNASUⁱ	80306.
Structural Biology Knowledgebase	Search...

Genome annotation databases

Ensemblⁱ	ENST00000237380; ENSP00000237380; ENSG00000118579.
GeneIDⁱ	80306.
KEGGⁱ	hsa:80306.
UCSC genome browser More...UCSCⁱ	uc003gpk.2. human.

Organism-specific databases

CTDⁱ	80306.
DisGeNET More...DisGeNETⁱ	80306.
GeneCardsⁱ	MED28.
Human Gene Nomenclature Database More...HGNCⁱ	HGNC:24628. MED28.
Human Protein Atlas More...HPAⁱ	HPA035900. HPA035901.
MIMⁱ	610311. gene.
neXtProtⁱ	NX_Q9H204.
Open Targets More...OpenTargetsⁱ	ENSG00000118579.
PharmGKBⁱ	PA134935853.
GenAtlas: human gene database More...GenAtlasⁱ	Search...

Phylogenomic databases

eggNOGⁱ	ENOG410IETV. Eukaryota. ENOG4111HUA. LUCA.
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00390000006192.
HOGENOMⁱ	HOG000113540.
HOVERGENⁱ	HBG052448.
InParanoidⁱ	Q9H204.
KEGG Orthology (KO) More...KOⁱ	K15141.
PhylomeDBⁱ	Q9H204.
TreeFamⁱ	TF326988.

Enzyme and pathway databases

Reactomeⁱ	R-HSA-1989781. PPARA activates gene expression. R-HSA-381340. Transcriptional regulation of white adipocyte differentiation.
SIGNORⁱ	Q9H204.

Miscellaneous databases

ChiTaRSⁱ	MED28. human.
GeneWikiⁱ	MED28.
GenomeRNAiⁱ	80306.
Protein Ontology More...PROⁱ	PR:Q9H204.
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSG00000118579.
CleanExⁱ	HS_MED28.
ExpressionAtlasⁱ	Q9H204. baseline and differential.
Genevisibleⁱ	Q9H204. HS.

Family and domain databases

InterProⁱ	View protein in InterPro IPR021640. Mediator_Med28.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF11594. Med28. 1 hit.
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	MED28_HUMAN
Accessionⁱ	Q9H204Primary (citable) accession number: Q9H204 Secondary accession number(s): Q9BZJ5
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	February 1, 2005
	Last sequence update:	March 1, 2001
	Last modified:	August 30, 2017
	This is version 134 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program
Disclaimer	Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

Documents

Human chromosome 4
Human chromosome 4: entries, gene names and cross-references to MIM
MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
SIMILARITY comments
Index of protein domains and families

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - Q9H204 (MED28_HUMAN)

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Mediator of RNA polymerase II transcription subunit 28

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Organism-specific databases

Polymorphism and mutation databases

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

Organism-specific databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Coiled coil

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including length and molecular weight.<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the ‘Sequence’ section reports difference(s) between the protein sequence shown in the UniProtKB entry and other available protein sequences derived from the same gene.<p><a href='/help/sequence_caution' target='_top'>More...</a></p>Sequence cautioni

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

<p>This subsection of the <a href="http://www.uniprot.org/manual/cross_references_section">Cross-references</a> section provides links to various web resources that are relevant for a specific protein.<p><a href='/help/web_resource' target='_top'>More...</a></p>Web resourcesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Polymorphism and mutation databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Documents

Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

GO - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Expressionⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Sequenceⁱ

Sequence cautionⁱ

Cross-referencesⁱ

Web resourcesⁱ

Entry informationⁱ

Miscellaneousⁱ