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Protein

Epsin-3

Gene

EPN3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei8 – 81Phosphatidylinositol lipid headgroupBy similarity
Binding sitei11 – 111Phosphatidylinositol lipid headgroupBy similarity
Binding sitei25 – 251Phosphatidylinositol lipid headgroupBy similarity
Binding sitei30 – 301Phosphatidylinositol lipid headgroupBy similarity
Binding sitei63 – 631Phosphatidylinositol lipid headgroupBy similarity
Binding sitei73 – 731Phosphatidylinositol lipid headgroupBy similarity

GO - Molecular functioni

Complete GO annotation...

Keywords - Ligandi

Lipid-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Epsin-3
Alternative name(s):
EPS-15-interacting protein 3
Gene namesi
Name:EPN3
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 17

Organism-specific databases

HGNCiHGNC:18235. EPN3.

Subcellular locationi

GO - Cellular componenti

  • clathrin-coated vesicle Source: UniProtKB
  • coated pit Source: Ensembl
  • extracellular exosome Source: UniProtKB
  • extrinsic component of plasma membrane Source: UniProtKB
  • intracellular membrane-bounded organelle Source: HPA
  • nucleoplasm Source: HPA
  • nucleus Source: UniProtKB
  • perinuclear region of cytoplasm Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoplasmic vesicle, Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA38513.

Polymorphism and mutation databases

BioMutaiEPN3.
DMDMi41017054.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 632632Epsin-3PRO_0000074519Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei191 – 1911PhosphoserineBy similarity
Modified residuei192 – 1921PhosphoserineBy similarity
Modified residuei264 – 2641PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9H201.
MaxQBiQ9H201.
PaxDbiQ9H201.
PeptideAtlasiQ9H201.
PRIDEiQ9H201.

PTM databases

iPTMnetiQ9H201.
PhosphoSiteiQ9H201.

Expressioni

Tissue specificityi

Detected in migrating keratinocytes from wounded skin, but not in differentiating keratinocytes or in normal skin. Detected in chronic wounds, basal cell carcinoma and ulcerative colitis.1 Publication

Inductioni

In keratinocytes, by wounding or contact with collagen.1 Publication

Gene expression databases

BgeeiQ9H201.
CleanExiHS_EPN3.
ExpressionAtlasiQ9H201. baseline and differential.
GenevisibleiQ9H201. HS.

Organism-specific databases

HPAiHPA055546.

Interactioni

Protein-protein interaction databases

BioGridi120369. 30 interactions.
IntActiQ9H201. 2 interactions.
MINTiMINT-1199053.
STRINGi9606.ENSP00000268933.

Structurei

3D structure databases

ProteinModelPortaliQ9H201.
SMRiQ9H201. Positions 12-155.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini12 – 144133ENTHPROSITE-ProRule annotationAdd
BLAST
Domaini209 – 22820UIM 1PROSITE-ProRule annotationAdd
BLAST
Domaini236 – 25520UIM 2PROSITE-ProRule annotationAdd
BLAST
Repeati321 – 32331
Repeati344 – 34632
Repeati371 – 37333
Repeati387 – 38934
Repeati404 – 40635
Repeati524 – 52631
Repeati537 – 53932
Repeati629 – 63133

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni321 – 406865 X 3 AA repeats of [DE]-P-WAdd
BLAST
Regioni524 – 6311083 X 3 AA repeats of N-P-FAdd
BLAST

Sequence similaritiesi

Belongs to the epsin family.Curated
Contains 1 ENTH (epsin N-terminal homology) domain.PROSITE-ProRule annotation
Contains 2 UIM (ubiquitin-interacting motif) domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG2056. Eukaryota.
ENOG410XSM0. LUCA.
GeneTreeiENSGT00550000074611.
HOGENOMiHOG000008298.
HOVERGENiHBG006690.
InParanoidiQ9H201.
KOiK12471.
OMAiADPWSPI.
OrthoDBiEOG7F511Z.
PhylomeDBiQ9H201.
TreeFamiTF313361.

Family and domain databases

Gene3Di1.25.40.90. 1 hit.
InterProiIPR013809. ENTH.
IPR008942. ENTH_VHS.
IPR027318. Epsin-3_metazoa.
IPR003903. UIM_dom.
[Graphical view]
PANTHERiPTHR12276:SF16. PTHR12276:SF16. 1 hit.
PfamiPF01417. ENTH. 1 hit.
PF02809. UIM. 2 hits.
[Graphical view]
SMARTiSM00273. ENTH. 1 hit.
SM00726. UIM. 2 hits.
[Graphical view]
SUPFAMiSSF48464. SSF48464. 1 hit.
PROSITEiPS50942. ENTH. 1 hit.
PS50330. UIM. 2 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9H201-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTTSALRRQV KNIVHNYSEA EIKVREATSN DPWGPPSSLM SEIADLTFNT
60 70 80 90 100
VAFTEVMGML WRRLNDSGKN WRHVYKALTL LDYLLKTGSE RVAHQCRENL
110 120 130 140 150
YTIQTLKDFQ YIDRDGKDQG VNVREKVKQV MALLKDEERL RQERTHALKT
160 170 180 190 200
KERMALEGIG IGSGQLGFSR RYGEDYSRSR GSPSSYNSSS SSPRYTSDLE
210 220 230 240 250
QARPQTSGEE ELQLQLALAM SREEAEKPVP PASHRDEDLQ LQLALRLSRQ
260 270 280 290 300
EHEKEVRSWQ GDGSPMANGA GAVVHHQRDR EPEREERKEE EKLKTSQSSI
310 320 330 340 350
LDLADIFVPA LAPPSTHCSA DPWDIPGFRP NTEASGSSWG PSADPWSPIP
360 370 380 390 400
SGTVLSRSQP WDLTPMLSSS EPWGRTPVLP AGPPTTDPWA LNSPHHKLPS
410 420 430 440 450
TGADPWGASL ETSDTPGGAS TFDPFAKPPE STETKEGLEQ ALPSGKPSSP
460 470 480 490 500
VELDLFGDPS PSSKQNGTKE PDALDLGILG EALTQPSKEA RACRTPESFL
510 520 530 540 550
GPSASSLVNL DSLVKAPQVA KTRNPFLTGL SAPSPTNPFG AGEPGRPTLN
560 570 580 590 600
QMRTGSPALG LAGGPVGAPL GSMTYSASLP LPLSSVPAGL TLPASVSVFP
610 620 630
QAGAFAPQPL LPTPSSAGPR PPPPQTGTNP FL
Length:632
Mass (Da):68,222
Last modified:March 1, 2001 - v1
Checksum:i13940F2FBD6215D0
GO
Isoform 2 (identifier: Q9H201-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     188-208: SSSSSPRYTSDLEQARPQTSG → CPASDVRGRGTAAAAGPRHEP
     209-632: Missing.

Note: No experimental confirmation available. May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.
Show »
Length:208
Mass (Da):23,608
Checksum:i4B45EB0984ED8081
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti375 – 3751R → K in BAF85666 (PubMed:14702039).Curated
Sequence conflicti396 – 3961H → Y in BAA91378 (PubMed:14702039).Curated
Sequence conflicti417 – 4171G → S in BAF85666 (PubMed:14702039).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti544 – 5441P → T.1 Publication
Corresponds to variant rs4794159 [ dbSNP | Ensembl ].
VAR_059973

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei188 – 20821SSSSS…PQTSG → CPASDVRGRGTAAAAGPRHE P in isoform 2. 1 PublicationVSP_009158Add
BLAST
Alternative sequencei209 – 632424Missing in isoform 2. 1 PublicationVSP_009159Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF324241 mRNA. Translation: AAG45223.1.
AK000785 mRNA. Translation: BAA91378.1.
AK291658 mRNA. Translation: BAF84347.1.
AK292977 mRNA. Translation: BAF85666.1.
CH471109 Genomic DNA. Translation: EAW94607.1.
BC001038 mRNA. No translation available.
BC051365 mRNA. No translation available.
BC077722 mRNA. No translation available.
CCDSiCCDS11570.1. [Q9H201-1]
RefSeqiNP_060427.2. NM_017957.2. [Q9H201-1]
UniGeneiHs.670090.

Genome annotation databases

EnsembliENST00000268933; ENSP00000268933; ENSG00000049283. [Q9H201-1]
ENST00000510045; ENSP00000421933; ENSG00000049283. [Q9H201-2]
GeneIDi55040.
KEGGihsa:55040.
UCSCiuc002ira.5. human. [Q9H201-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF324241 mRNA. Translation: AAG45223.1.
AK000785 mRNA. Translation: BAA91378.1.
AK291658 mRNA. Translation: BAF84347.1.
AK292977 mRNA. Translation: BAF85666.1.
CH471109 Genomic DNA. Translation: EAW94607.1.
BC001038 mRNA. No translation available.
BC051365 mRNA. No translation available.
BC077722 mRNA. No translation available.
CCDSiCCDS11570.1. [Q9H201-1]
RefSeqiNP_060427.2. NM_017957.2. [Q9H201-1]
UniGeneiHs.670090.

3D structure databases

ProteinModelPortaliQ9H201.
SMRiQ9H201. Positions 12-155.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi120369. 30 interactions.
IntActiQ9H201. 2 interactions.
MINTiMINT-1199053.
STRINGi9606.ENSP00000268933.

PTM databases

iPTMnetiQ9H201.
PhosphoSiteiQ9H201.

Polymorphism and mutation databases

BioMutaiEPN3.
DMDMi41017054.

Proteomic databases

EPDiQ9H201.
MaxQBiQ9H201.
PaxDbiQ9H201.
PeptideAtlasiQ9H201.
PRIDEiQ9H201.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000268933; ENSP00000268933; ENSG00000049283. [Q9H201-1]
ENST00000510045; ENSP00000421933; ENSG00000049283. [Q9H201-2]
GeneIDi55040.
KEGGihsa:55040.
UCSCiuc002ira.5. human. [Q9H201-1]

Organism-specific databases

CTDi55040.
GeneCardsiEPN3.
HGNCiHGNC:18235. EPN3.
HPAiHPA055546.
MIMi607264. gene.
neXtProtiNX_Q9H201.
PharmGKBiPA38513.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2056. Eukaryota.
ENOG410XSM0. LUCA.
GeneTreeiENSGT00550000074611.
HOGENOMiHOG000008298.
HOVERGENiHBG006690.
InParanoidiQ9H201.
KOiK12471.
OMAiADPWSPI.
OrthoDBiEOG7F511Z.
PhylomeDBiQ9H201.
TreeFamiTF313361.

Miscellaneous databases

GenomeRNAii55040.
PROiQ9H201.
SOURCEiSearch...

Gene expression databases

BgeeiQ9H201.
CleanExiHS_EPN3.
ExpressionAtlasiQ9H201. baseline and differential.
GenevisibleiQ9H201. HS.

Family and domain databases

Gene3Di1.25.40.90. 1 hit.
InterProiIPR013809. ENTH.
IPR008942. ENTH_VHS.
IPR027318. Epsin-3_metazoa.
IPR003903. UIM_dom.
[Graphical view]
PANTHERiPTHR12276:SF16. PTHR12276:SF16. 1 hit.
PfamiPF01417. ENTH. 1 hit.
PF02809. UIM. 2 hits.
[Graphical view]
SMARTiSM00273. ENTH. 1 hit.
SM00726. UIM. 2 hits.
[Graphical view]
SUPFAMiSSF48464. SSF48464. 1 hit.
PROSITEiPS50942. ENTH. 1 hit.
PS50330. UIM. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Epsin 3 is a novel extracellular matrix-induced transcript specific to wounded epithelia."
    Spradling K.D., McDaniel A.E., Lohi J., Pilcher B.K.
    J. Biol. Chem. 276:29257-29267(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), INDUCTION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
    Tissue: Keratinocyte.
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT THR-544.
    Tissue: Colon, Placenta and Trachea.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Placenta.

Entry informationi

Entry nameiEPN3_HUMAN
AccessioniPrimary (citable) accession number: Q9H201
Secondary accession number(s): A8K6J3
, A8KAB2, Q9BVN6, Q9NWK2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 16, 2004
Last sequence update: March 1, 2001
Last modified: July 6, 2016
This is version 130 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.