Q9H1Y3 (OPN3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Opsin-3

Alternative name(s):
Encephalopsin
Panopsin

Gene names

Name:	OPN3
Synonyms:	ECPN

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	402 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May play a role in encephalic photoreception.
Subcellular location	Membrane; Multi-pass membrane protein.
Tissue specificity	Strongly expressed in brain. Highly expressed in the preoptic area and paraventricular nucleus of the hypothalamus. Shows highly patterned expression in other regions of the brain, being enriched in selected regions of the cerebral cortex, cerebellar Purkinje cells, a subset of striatal neurons, selected thalamic nuclei, and a subset of interneurons in the ventral horn of the spinal cord.
Sequence similarities	Belongs to the G-protein coupled receptor 1 family. Opsin subfamily.

Ontologies

Keywords
Biological process	Sensory transduction
Cellular component	Membrane
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Transmembrane Transmembrane helix
Ligand	Chromophore
Molecular function	G-protein coupled receptor Photoreceptor protein Receptor Retinal protein Transducer
PTM	Disulfide bond Glycoprotein Lipoprotein Palmitate
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	phototransduction Non-traceable author statement Ref.1. Source: UniProtKB protein-chromophore linkage Inferred from electronic annotation. Source: UniProtKB-KW regulation of circadian rhythm Non-traceable author statement Ref.1. Source: UniProtKB visual perception Inferred from electronic annotation. Source: InterPro
Cellular_component	integral to plasma membrane Traceable author statement Ref.1. Source: ProtInc
Molecular_function	G-protein coupled photoreceptor activity Non-traceable author statement Ref.1. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9H1Y3-1)

Also known as: 1-4b-5-6;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9H1Y3-2)

Also known as: 1-2-5-6;

The sequence of this isoform differs from the canonical sequence as follows:
     126-231: IVSIATLTVL...YGHILYSIRM → WISQLQAATREARASMGPVQQGTICMQ

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 402	402	Opsin-3		PRO_0000197813
Regions
	Topological domain	1 – 40	40	Extracellular Potential
	Transmembrane	41 – 65	25	Helical; Name=1; Potential
	Topological domain	66 – 77	12	Cytoplasmic Potential
	Transmembrane	78 – 102	25	Helical; Name=2; Potential
	Topological domain	103 – 117	15	Extracellular Potential
	Transmembrane	118 – 137	20	Helical; Name=3; Potential
	Topological domain	138 – 153	16	Cytoplasmic Potential
	Transmembrane	154 – 177	24	Helical; Name=4; Potential
	Topological domain	178 – 201	24	Extracellular Potential
	Transmembrane	202 – 229	28	Helical; Name=5; Potential
	Topological domain	230 – 255	26	Cytoplasmic Potential
	Transmembrane	256 – 279	24	Helical; Name=6; Potential
	Topological domain	280 – 287	8	Extracellular Potential
	Transmembrane	288 – 312	25	Helical; Name=7; Potential
	Topological domain	313 – 402	90	Cytoplasmic Potential
Amino acid modifications
	Modified residue	299	1	N6-(retinylidene)lysine
	Lipidation	325	1	S-palmitoyl cysteine By similarity
	Glycosylation	5	1	N-linked (GlcNAc...) Potential
	Glycosylation	198	1	N-linked (GlcNAc...) Potential
	Disulfide bond	114 ↔ 188		By similarity
Natural variations
	Alternative sequence	126 – 231	106	IVSIA…YSIRM → WISQLQAATREARASMGPVQ QGTICMQ in isoform 2.		VSP_010209
	Natural variant	167	1	A → V. Corresponds to variant rs12072790 [ dbSNP | Ensembl ].		VAR_050613
	Natural variant	183	1	V → I. Corresponds to variant rs2273712 [ dbSNP | Ensembl ].		VAR_050614
Experimental info
	Sequence conflict	390 – 396	7	NGSKVDV → IGVQSLML in AAD32671. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (1-4b-5-6) [UniParc]. Last modified March 1, 2001. Version 1. Checksum: 370F64C19F834A71	FASTA	402	44,873
	10 20 30 40 50 60 MYSGNRSGGH GYWDGGGAAG AEGPAPAGTL SPAPLFSPGT YERLALLLGS IGLLGVGNNL 70 80 90 100 110 120 LVLVLYYKFQ RLRTPTHLLL VNISLSDLLV SLFGVTFTFV SCLRNGWVWD TVGCVWDGFS 130 140 150 160 170 180 GSLFGIVSIA TLTVLAYERY IRVVHARVIN FSWAWRAITY IWLYSLAWAG APLLGWNRYI 190 200 210 220 230 240 LDVHGLGCTV DWKSKDANDS SFVLFLFLGC LVVPLGVIAH CYGHILYSIR MLRCVEDLQT 250 260 270 280 290 300 IQVIKILKYE KKLAKMCFLM IFTFLVCWMP YIVICFLVVN GHGHLVTPTI SIVSYLFAKS 310 320 330 340 350 360 NTVYNPVIYV FMIRKFRRSL LQLLCLRLLR CQRPAKDLPA AGSEMQIRPI VMSQKDGDRP 370 380 390 400 KKKVTFNSSS IIFIITSDES LSVDDSDKTN GSKVDVIQVR PL
	Isoform 2 (1-2-5-6) [UniParc]. Checksum: BA9734383981FF2F	FASTA	323	35,730
	10 20 30 40 50 60 MYSGNRSGGH GYWDGGGAAG AEGPAPAGTL SPAPLFSPGT YERLALLLGS IGLLGVGNNL 70 80 90 100 110 120 LVLVLYYKFQ RLRTPTHLLL VNISLSDLLV SLFGVTFTFV SCLRNGWVWD TVGCVWDGFS 130 140 150 160 170 180 GSLFGWISQL QAATREARAS MGPVQQGTIC MQLRCVEDLQ TIQVIKILKY EKKLAKMCFL 190 200 210 220 230 240 MIFTFLVCWM PYIVICFLVV NGHGHLVTPT ISIVSYLFAK SNTVYNPVIY VFMIRKFRRS 250 260 270 280 290 300 LLQLLCLRLL RCQRPAKDLP AAGSEMQIRP IVMSQKDGDR PKKKVTFNSS SIIFIITSDE 310 320 SLSVDDSDKT NGSKVDVIQV RPL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Encephalopsin: a novel mammalian extraretinal opsin discretely localized in the brain." Blackshaw S., Snyder S.H. J. Neurosci. 19:3681-3690(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]	"Characterization of a novel human opsin gene with wide tissue expression and identification of embedded and flanking genes on chromosome 1q43." Halford S., Freedman M.S., Bellingham J., Inglis S.L., Poopalasundaram S., Soni B.G., Foster R.G., Hunt D.M. Genomics 72:203-208(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[3]	"Different structural organization of the encephalopsin gene in man and mouse." Kasper G., Taudien S., Staub E., Mennerich D., Rieder M., Hinzmann B., Dahl E., Schwidetzky U., Rosenthal A., Rump A. Gene 295:27-32(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], ALTERNATIVE SPLICING.
[4]	"The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R. Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Ovary.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF140242 mRNA. Translation: AAD32671.1. AF303588 mRNA. Translation: AAK37447.1. AF482426 Genomic DNA. Translation: AAO15715.1. AF482426 Genomic DNA. Translation: AAO15717.1. AL133390 Genomic DNA. No translation available. BC036773 mRNA. Translation: AAH36773.1.
IPI	IPI00217348. IPI00300717.
RefSeq	NP_055137.2. NM_014322.2.
UniGene	Hs.409081.
3D structure databases
ProteinModelPortal	Q9H1Y3.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000355512.
Protein family/group databases
GPCRDB	Search...
PTM databases
PhosphoSite	Q9H1Y3.
Polymorphism databases
DMDM	17380172.
Proteomic databases
PaxDb	Q9H1Y3.
PRIDE	Q9H1Y3.
Protocols and materials databases
DNASU	23596.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000331838; ENSP00000328018; ENSG00000054277. ENST00000366554; ENSP00000355512; ENSG00000054277.
GeneID	23596.
KEGG	hsa:23596.
UCSC	uc001hza.3. human.
Organism-specific databases
CTD	23596.
GeneCards	GC01M241753.
HGNC	HGNC:14007. OPN3.
HPA	CAB013682.
MIM	606695. gene.
neXtProt	NX_Q9H1Y3.
PharmGKB	PA31939.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG250907.
HOGENOM	HOG000029042.
HOVERGEN	HBG100337.
InParanoid	Q9H1Y3.
KO	K04256.
OMA	TFLICWM.
PhylomeDB	Q9H1Y3.
Enzyme and pathway databases
Reactome	REACT_111102. Signal Transduction.
Gene expression databases
Bgee	Q9H1Y3.
CleanEx	HS_OPN3.
Genevestigator	Q9H1Y3.
GermOnline	ENSG00000054277. Homo sapiens.
Family and domain databases
InterPro	IPR000276. GPCR_Rhodpsn. IPR017452. GPCR_Rhodpsn_7TM. IPR001760. Opsin. [Graphical view]
Pfam	PF00001. 7tm_1. 1 hit. [Graphical view]
PRINTS	PR00237. GPCRRHODOPSN.
PROSITE	PS00237. G_PROTEIN_RECEP_F1_1. 1 hit. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. PS00238. OPSIN. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	23596.
NextBio	46258.
SOURCE	Search...

Entry information

Entry name

OPN3_HUMAN

Accession

Primary (citable) accession number: Q9H1Y3
Secondary accession number(s): Q8IX08, Q9Y344

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 26, 2001
Last sequence update:	March 1, 2001
Last modified:	May 1, 2013
This is version 110 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.