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Protein

Adenosine 3'-phospho 5'-phosphosulfate transporter 2

Gene

SLC35B3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Mediates the transport of adenosine 3'-phospho 5'-phosphosulfate (PAPS), from cytosol into Golgi. PAPS is a universal sulfuryl donor for sulfation events that take place in the Golgi. Compensates for the insufficient expression of SLC35B2/PAPST1 during the synthesis of sulfated glycoconjugates in the colon.1 Publication

Kineticsi

  1. KM=2.2 µM for PAPS1 Publication

    GO - Molecular functioni

    GO - Biological processi

    Keywordsi

    Biological processTransport

    Enzyme and pathway databases

    ReactomeiR-HSA-174362 Transport and synthesis of PAPS
    R-HSA-727802 Transport of nucleotide sugars

    Protein family/group databases

    TCDBi2.A.7.11.5 the drug/metabolite transporter (dmt) superfamily

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Adenosine 3'-phospho 5'-phosphosulfate transporter 2
    Alternative name(s):
    3'-phosphoadenosine 5'-phosphosulfate transporter
    PAPS transporter 2
    Solute carrier family 35 member B3
    Gene namesi
    Name:SLC35B3
    Synonyms:C6orf196, PAPST2
    ORF Names:CGI-19
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    Proteomesi
    • UP000005640 Componenti: Chromosome 6

    Organism-specific databases

    EuPathDBiHostDB:ENSG00000124786.9
    HGNCiHGNC:21601 SLC35B3
    MIMi610845 gene
    neXtProtiNX_Q9H1N7

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

    Topology

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Transmembranei78 – 98HelicalSequence analysisAdd BLAST21
    Transmembranei114 – 134HelicalSequence analysisAdd BLAST21
    Transmembranei147 – 167HelicalSequence analysisAdd BLAST21
    Transmembranei170 – 190HelicalSequence analysisAdd BLAST21
    Transmembranei196 – 216HelicalSequence analysisAdd BLAST21
    Transmembranei223 – 243HelicalSequence analysisAdd BLAST21
    Transmembranei267 – 287HelicalSequence analysisAdd BLAST21
    Transmembranei298 – 317HelicalSequence analysisAdd BLAST20
    Transmembranei324 – 346HelicalSequence analysisAdd BLAST23
    Transmembranei349 – 369HelicalSequence analysisAdd BLAST21

    Keywords - Cellular componenti

    Golgi apparatus, Membrane

    Pathology & Biotechi

    Organism-specific databases

    DisGeNETi51000
    OpenTargetsiENSG00000124786
    PharmGKBiPA134889889

    Polymorphism and mutation databases

    DMDMi74752580

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00002133791 – 401Adenosine 3'-phospho 5'-phosphosulfate transporter 2Add BLAST401

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Glycosylationi12N-linked (GlcNAc...) asparagineSequence analysis1
    Glycosylationi71N-linked (GlcNAc...) asparagineSequence analysis1
    Glycosylationi254N-linked (GlcNAc...) asparagineSequence analysis1

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    EPDiQ9H1N7
    MaxQBiQ9H1N7
    PaxDbiQ9H1N7
    PeptideAtlasiQ9H1N7
    PRIDEiQ9H1N7

    PTM databases

    iPTMnetiQ9H1N7
    PhosphoSitePlusiQ9H1N7

    Expressioni

    Tissue specificityi

    Preferentially and highly expressed in colon.1 Publication

    Gene expression databases

    BgeeiENSG00000124786
    CleanExiHS_SLC35B3
    ExpressionAtlasiQ9H1N7 baseline and differential
    GenevisibleiQ9H1N7 HS

    Organism-specific databases

    HPAiHPA054661
    HPA057801

    Interactioni

    Protein-protein interaction databases

    BioGridi119208, 2 interactors
    IntActiQ9H1N7, 1 interactor
    STRINGi9606.ENSP00000368981

    Structurei

    3D structure databases

    ProteinModelPortaliQ9H1N7
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiKOG1582 Eukaryota
    COG0697 LUCA
    GeneTreeiENSGT00530000063090
    HOGENOMiHOG000006136
    HOVERGENiHBG066829
    KOiK15277
    OMAiYNRTTQF
    OrthoDBiEOG091G0CL7
    PhylomeDBiQ9H1N7
    TreeFamiTF314523

    Family and domain databases

    InterProiView protein in InterPro
    IPR013657 UAA
    PfamiView protein in Pfam
    PF08449 UAA, 1 hit

    Sequences (3)i

    Sequence statusi: Complete.

    This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

    Isoform 1 (identifier: Q9H1N7-1) [UniParc]FASTAAdd to basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

            10         20         30         40         50
    MDLTQQAKDI QNITVQETNK NNSESIECSK ITMDLKFNNS RKYISITVPS
    60 70 80 90 100
    KTQTMSPHIK SVDDVVVLGM NLSKFNKLTQ FFICVAGVFV FYLIYGYLQE
    110 120 130 140 150
    LIFSVEGFKS CGWYLTLVQF AFYSIFGLIE LQLIQDKRRR IPGKTYMIIA
    160 170 180 190 200
    FLTVGTMGLS NTSLGYLNYP TQVIFKCCKL IPVMLGGVFI QGKRYNVADV
    210 220 230 240 250
    SAAICMSLGL IWFTLADSTT APNFNLTGVV LISLALCADA VIGNVQEKAM
    260 270 280 290 300
    KLHNASNSEM VLYSYSIGFV YILLGLTCTS GLGPAVTFCA KNPVRTYGYA
    310 320 330 340 350
    FLFSLTGYFG ISFVLALIKI FGALIAVTVT TGRKAMTIVL SFIFFAKPFT
    360 370 380 390 400
    FQYVWSGLLV VLGIFLNVYS KNMDKIRLPS LYDLINKSVE ARKSRTLAQT

    V
    Length:401
    Mass (Da):44,593
    Last modified:March 1, 2001 - v1
    Checksum:i2582EFD45ECA842C
    GO
    Isoform 2 (identifier: Q9H1N7-2) [UniParc]FASTAAdd to basket

    The sequence of this isoform differs from the canonical sequence as follows:
         141-153: IPGKTYMIIAFLT → YVVCFYFLIFHLY
         154-401: Missing.

    Note: No experimental confirmation available.
    Show »
    Length:153
    Mass (Da):17,914
    Checksum:iAEF35780DBBDA8E2
    GO
    Isoform 3 (identifier: Q9H1N7-3) [UniParc]FASTAAdd to basket

    The sequence of this isoform differs from the canonical sequence as follows:
         261-283: VLYSYSIGFVYILLGLTCTSGLG → NITTSSYSGVFIFPNKLKIPLVL
         284-401: Missing.

    Note: No experimental confirmation available.
    Show »
    Length:283
    Mass (Da):31,531
    Checksum:i7A9DF4B6ACC02E39
    GO

    Sequence cautioni

    The sequence AAD27728 differs from that shown. Reason: Frameshift at several positions.Curated
    The sequence CAE46041 differs from that shown. Reason: Erroneous initiation.Curated

    Alternative sequence

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Alternative sequenceiVSP_016193141 – 153IPGKT…IAFLT → YVVCFYFLIFHLY in isoform 2. 1 PublicationAdd BLAST13
    Alternative sequenceiVSP_016194154 – 401Missing in isoform 2. 1 PublicationAdd BLAST248
    Alternative sequenceiVSP_016195261 – 283VLYSY…TSGLG → NITTSSYSGVFIFPNKLKIP LVL in isoform 3. 1 PublicationAdd BLAST23
    Alternative sequenceiVSP_016196284 – 401Missing in isoform 3. 1 PublicationAdd BLAST118

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AB231931 mRNA Translation: BAE93015.1
    AF132953 mRNA Translation: AAD27728.1 Frameshift.
    BX538271 mRNA Translation: CAD98078.1
    BX641086 mRNA Translation: CAE46041.1 Different initiation.
    AL355815 Genomic DNA No translation available.
    BC006973 mRNA Translation: AAH06973.1
    CCDSiCCDS4508.1 [Q9H1N7-1]
    RefSeqiNP_001136013.1, NM_001142541.2 [Q9H1N7-1]
    NP_057032.2, NM_015948.4 [Q9H1N7-1]
    XP_005249214.1, XM_005249157.2 [Q9H1N7-1]
    XP_006715165.1, XM_006715102.3 [Q9H1N7-1]
    XP_016866399.1, XM_017010910.1 [Q9H1N7-1]
    XP_016866400.1, XM_017010911.1 [Q9H1N7-1]
    UniGeneiHs.285847

    Genome annotation databases

    EnsembliENST00000379660; ENSP00000368981; ENSG00000124786 [Q9H1N7-1]
    GeneIDi51000
    KEGGihsa:51000
    UCSCiuc003myb.5 human [Q9H1N7-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Similar proteinsi

    Entry informationi

    Entry nameiS35B3_HUMAN
    AccessioniPrimary (citable) accession number: Q9H1N7
    Secondary accession number(s): A6NKX9
    , Q1XH11, Q6MZJ0, Q7Z662, Q9Y308
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 22, 2005
    Last sequence update: March 1, 2001
    Last modified: January 31, 2018
    This is version 129 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome
    UniProt is an ELIXIR core data resource
    Main funding by: National Institutes of Health