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Protein

Adenosine 3'-phospho 5'-phosphosulfate transporter 2

Gene

SLC35B3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Mediates the transport of adenosine 3'-phospho 5'-phosphosulfate (PAPS), from cytosol into Golgi. PAPS is a universal sulfuryl donor for sulfation events that take place in the Golgi. Compensates for the insufficient expression of SLC35B2/PAPST1 during the synthesis of sulfated glycoconjugates in the colon.1 Publication

Kineticsi

  1. KM=2.2 µM for PAPS1 Publication

    GO - Molecular functioni

    GO - Biological processi

    Complete GO annotation...

    Keywords - Biological processi

    Transport

    Enzyme and pathway databases

    BioCyciZFISH:ENSG00000124786-MONOMER.
    ReactomeiR-HSA-174362. Transport and synthesis of PAPS.
    R-HSA-727802. Transport of nucleotide sugars.

    Protein family/group databases

    TCDBi2.A.7.11.5. the drug/metabolite transporter (dmt) superfamily.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Adenosine 3'-phospho 5'-phosphosulfate transporter 2
    Alternative name(s):
    3'-phosphoadenosine 5'-phosphosulfate transporter
    PAPS transporter 2
    Solute carrier family 35 member B3
    Gene namesi
    Name:SLC35B3
    Synonyms:C6orf196, PAPST2
    ORF Names:CGI-19
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    Proteomesi
    • UP000005640 Componenti: Chromosome 6

    Organism-specific databases

    HGNCiHGNC:21601. SLC35B3.

    Subcellular locationi

    Topology

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Transmembranei78 – 98HelicalSequence analysisAdd BLAST21
    Transmembranei114 – 134HelicalSequence analysisAdd BLAST21
    Transmembranei147 – 167HelicalSequence analysisAdd BLAST21
    Transmembranei170 – 190HelicalSequence analysisAdd BLAST21
    Transmembranei196 – 216HelicalSequence analysisAdd BLAST21
    Transmembranei223 – 243HelicalSequence analysisAdd BLAST21
    Transmembranei267 – 287HelicalSequence analysisAdd BLAST21
    Transmembranei298 – 317HelicalSequence analysisAdd BLAST20
    Transmembranei324 – 346HelicalSequence analysisAdd BLAST23
    Transmembranei349 – 369HelicalSequence analysisAdd BLAST21

    GO - Cellular componenti

    Complete GO annotation...

    Keywords - Cellular componenti

    Golgi apparatus, Membrane

    Pathology & Biotechi

    Organism-specific databases

    DisGeNETi51000.
    OpenTargetsiENSG00000124786.
    PharmGKBiPA134889889.

    Polymorphism and mutation databases

    DMDMi74752580.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00002133791 – 401Adenosine 3'-phospho 5'-phosphosulfate transporter 2Add BLAST401

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Glycosylationi12N-linked (GlcNAc...)Sequence analysis1
    Glycosylationi71N-linked (GlcNAc...)Sequence analysis1
    Glycosylationi254N-linked (GlcNAc...)Sequence analysis1

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    EPDiQ9H1N7.
    MaxQBiQ9H1N7.
    PaxDbiQ9H1N7.
    PeptideAtlasiQ9H1N7.
    PRIDEiQ9H1N7.

    PTM databases

    iPTMnetiQ9H1N7.
    PhosphoSitePlusiQ9H1N7.

    Expressioni

    Tissue specificityi

    Preferentially and highly expressed in colon.1 Publication

    Gene expression databases

    BgeeiENSG00000124786.
    CleanExiHS_SLC35B3.
    ExpressionAtlasiQ9H1N7. baseline and differential.
    GenevisibleiQ9H1N7. HS.

    Organism-specific databases

    HPAiHPA054661.
    HPA057801.

    Interactioni

    Protein-protein interaction databases

    BioGridi119208. 2 interactors.
    IntActiQ9H1N7. 1 interactor.
    STRINGi9606.ENSP00000368981.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9H1N7.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiKOG1582. Eukaryota.
    COG0697. LUCA.
    GeneTreeiENSGT00530000063090.
    HOGENOMiHOG000006136.
    HOVERGENiHBG066829.
    KOiK15277.
    OMAiFTFQYIW.
    OrthoDBiEOG091G0CL7.
    PhylomeDBiQ9H1N7.
    TreeFamiTF314523.

    Family and domain databases

    InterProiIPR013657. UAA.
    [Graphical view]
    PfamiPF08449. UAA. 1 hit.
    [Graphical view]

    Sequences (3)i

    Sequence statusi: Complete.

    This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

    Isoform 1 (identifier: Q9H1N7-1) [UniParc]FASTAAdd to basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

            10         20         30         40         50
    MDLTQQAKDI QNITVQETNK NNSESIECSK ITMDLKFNNS RKYISITVPS
    60 70 80 90 100
    KTQTMSPHIK SVDDVVVLGM NLSKFNKLTQ FFICVAGVFV FYLIYGYLQE
    110 120 130 140 150
    LIFSVEGFKS CGWYLTLVQF AFYSIFGLIE LQLIQDKRRR IPGKTYMIIA
    160 170 180 190 200
    FLTVGTMGLS NTSLGYLNYP TQVIFKCCKL IPVMLGGVFI QGKRYNVADV
    210 220 230 240 250
    SAAICMSLGL IWFTLADSTT APNFNLTGVV LISLALCADA VIGNVQEKAM
    260 270 280 290 300
    KLHNASNSEM VLYSYSIGFV YILLGLTCTS GLGPAVTFCA KNPVRTYGYA
    310 320 330 340 350
    FLFSLTGYFG ISFVLALIKI FGALIAVTVT TGRKAMTIVL SFIFFAKPFT
    360 370 380 390 400
    FQYVWSGLLV VLGIFLNVYS KNMDKIRLPS LYDLINKSVE ARKSRTLAQT

    V
    Length:401
    Mass (Da):44,593
    Last modified:March 1, 2001 - v1
    Checksum:i2582EFD45ECA842C
    GO
    Isoform 2 (identifier: Q9H1N7-2) [UniParc]FASTAAdd to basket

    The sequence of this isoform differs from the canonical sequence as follows:
         141-153: IPGKTYMIIAFLT → YVVCFYFLIFHLY
         154-401: Missing.

    Note: No experimental confirmation available.
    Show »
    Length:153
    Mass (Da):17,914
    Checksum:iAEF35780DBBDA8E2
    GO
    Isoform 3 (identifier: Q9H1N7-3) [UniParc]FASTAAdd to basket

    The sequence of this isoform differs from the canonical sequence as follows:
         261-283: VLYSYSIGFVYILLGLTCTSGLG → NITTSSYSGVFIFPNKLKIPLVL
         284-401: Missing.

    Note: No experimental confirmation available.
    Show »
    Length:283
    Mass (Da):31,531
    Checksum:i7A9DF4B6ACC02E39
    GO

    Sequence cautioni

    The sequence AAD27728 differs from that shown. Reason: Frameshift at several positions.Curated
    The sequence CAE46041 differs from that shown. Reason: Erroneous initiation.Curated

    Alternative sequence

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Alternative sequenceiVSP_016193141 – 153IPGKT…IAFLT → YVVCFYFLIFHLY in isoform 2. 1 PublicationAdd BLAST13
    Alternative sequenceiVSP_016194154 – 401Missing in isoform 2. 1 PublicationAdd BLAST248
    Alternative sequenceiVSP_016195261 – 283VLYSY…TSGLG → NITTSSYSGVFIFPNKLKIP LVL in isoform 3. 1 PublicationAdd BLAST23
    Alternative sequenceiVSP_016196284 – 401Missing in isoform 3. 1 PublicationAdd BLAST118

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AB231931 mRNA. Translation: BAE93015.1.
    AF132953 mRNA. Translation: AAD27728.1. Frameshift.
    BX538271 mRNA. Translation: CAD98078.1.
    BX641086 mRNA. Translation: CAE46041.1. Different initiation.
    AL355815 Genomic DNA. Translation: CAC19504.1.
    BC006973 mRNA. Translation: AAH06973.1.
    CCDSiCCDS4508.1. [Q9H1N7-1]
    RefSeqiNP_001136013.1. NM_001142541.2. [Q9H1N7-1]
    NP_057032.2. NM_015948.4. [Q9H1N7-1]
    XP_005249214.1. XM_005249157.2. [Q9H1N7-1]
    XP_006715165.1. XM_006715102.3. [Q9H1N7-1]
    XP_016866399.1. XM_017010910.1. [Q9H1N7-1]
    XP_016866400.1. XM_017010911.1. [Q9H1N7-1]
    UniGeneiHs.285847.

    Genome annotation databases

    EnsembliENST00000379660; ENSP00000368981; ENSG00000124786. [Q9H1N7-1]
    GeneIDi51000.
    KEGGihsa:51000.
    UCSCiuc003myb.5. human. [Q9H1N7-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AB231931 mRNA. Translation: BAE93015.1.
    AF132953 mRNA. Translation: AAD27728.1. Frameshift.
    BX538271 mRNA. Translation: CAD98078.1.
    BX641086 mRNA. Translation: CAE46041.1. Different initiation.
    AL355815 Genomic DNA. Translation: CAC19504.1.
    BC006973 mRNA. Translation: AAH06973.1.
    CCDSiCCDS4508.1. [Q9H1N7-1]
    RefSeqiNP_001136013.1. NM_001142541.2. [Q9H1N7-1]
    NP_057032.2. NM_015948.4. [Q9H1N7-1]
    XP_005249214.1. XM_005249157.2. [Q9H1N7-1]
    XP_006715165.1. XM_006715102.3. [Q9H1N7-1]
    XP_016866399.1. XM_017010910.1. [Q9H1N7-1]
    XP_016866400.1. XM_017010911.1. [Q9H1N7-1]
    UniGeneiHs.285847.

    3D structure databases

    ProteinModelPortaliQ9H1N7.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi119208. 2 interactors.
    IntActiQ9H1N7. 1 interactor.
    STRINGi9606.ENSP00000368981.

    Protein family/group databases

    TCDBi2.A.7.11.5. the drug/metabolite transporter (dmt) superfamily.

    PTM databases

    iPTMnetiQ9H1N7.
    PhosphoSitePlusiQ9H1N7.

    Polymorphism and mutation databases

    DMDMi74752580.

    Proteomic databases

    EPDiQ9H1N7.
    MaxQBiQ9H1N7.
    PaxDbiQ9H1N7.
    PeptideAtlasiQ9H1N7.
    PRIDEiQ9H1N7.

    Protocols and materials databases

    DNASUi51000.
    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsembliENST00000379660; ENSP00000368981; ENSG00000124786. [Q9H1N7-1]
    GeneIDi51000.
    KEGGihsa:51000.
    UCSCiuc003myb.5. human. [Q9H1N7-1]

    Organism-specific databases

    CTDi51000.
    DisGeNETi51000.
    GeneCardsiSLC35B3.
    HGNCiHGNC:21601. SLC35B3.
    HPAiHPA054661.
    HPA057801.
    MIMi610845. gene.
    neXtProtiNX_Q9H1N7.
    OpenTargetsiENSG00000124786.
    PharmGKBiPA134889889.
    GenAtlasiSearch...

    Phylogenomic databases

    eggNOGiKOG1582. Eukaryota.
    COG0697. LUCA.
    GeneTreeiENSGT00530000063090.
    HOGENOMiHOG000006136.
    HOVERGENiHBG066829.
    KOiK15277.
    OMAiFTFQYIW.
    OrthoDBiEOG091G0CL7.
    PhylomeDBiQ9H1N7.
    TreeFamiTF314523.

    Enzyme and pathway databases

    BioCyciZFISH:ENSG00000124786-MONOMER.
    ReactomeiR-HSA-174362. Transport and synthesis of PAPS.
    R-HSA-727802. Transport of nucleotide sugars.

    Miscellaneous databases

    ChiTaRSiSLC35B3. human.
    GenomeRNAii51000.
    PROiQ9H1N7.
    SOURCEiSearch...

    Gene expression databases

    BgeeiENSG00000124786.
    CleanExiHS_SLC35B3.
    ExpressionAtlasiQ9H1N7. baseline and differential.
    GenevisibleiQ9H1N7. HS.

    Family and domain databases

    InterProiIPR013657. UAA.
    [Graphical view]
    PfamiPF08449. UAA. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Entry informationi

    Entry nameiS35B3_HUMAN
    AccessioniPrimary (citable) accession number: Q9H1N7
    Secondary accession number(s): A6NKX9
    , Q1XH11, Q6MZJ0, Q7Z662, Q9Y308
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 22, 2005
    Last sequence update: March 1, 2001
    Last modified: November 2, 2016
    This is version 123 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 6
      Human chromosome 6: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.