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Q9H1N7

- S35B3_HUMAN

UniProt

Q9H1N7 - S35B3_HUMAN

Protein

Adenosine 3'-phospho 5'-phosphosulfate transporter 2

Gene

SLC35B3

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 103 (01 Oct 2014)
      Sequence version 1 (01 Mar 2001)
      Previous versions | rss
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    Functioni

    Mediates the transport of adenosine 3'-phospho 5'-phosphosulfate (PAPS), from cytosol into Golgi. PAPS is a universal sulfuryl donor for sulfation events that take place in the Golgi. Compensates for the insufficient expression of SLC35B2/PAPST1 during the synthesis of sulfated glycoconjugates in the colon.1 Publication

    Kineticsi

    1. KM=2.2 µM for PAPS1 Publication

    GO - Biological processi

    1. 3'-phosphoadenosine 5'-phosphosulfate biosynthetic process Source: Reactome
    2. 3'-phosphoadenosine 5'-phosphosulfate metabolic process Source: Reactome
    3. carbohydrate metabolic process Source: Reactome
    4. glycosaminoglycan metabolic process Source: Reactome
    5. small molecule metabolic process Source: Reactome
    6. transmembrane transport Source: Reactome
    7. xenobiotic metabolic process Source: Reactome

    Keywords - Biological processi

    Transport

    Enzyme and pathway databases

    ReactomeiREACT_22151. Transport of nucleotide sugars.
    REACT_6840. Transport and synthesis of PAPS.

    Protein family/group databases

    TCDBi2.A.7.11.5. the drug/metabolite transporter (dmt) superfamily.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Adenosine 3'-phospho 5'-phosphosulfate transporter 2
    Alternative name(s):
    3'-phosphoadenosine 5'-phosphosulfate transporter
    PAPS transporter 2
    Solute carrier family 35 member B3
    Gene namesi
    Name:SLC35B3
    Synonyms:C6orf196, PAPST2
    ORF Names:CGI-19
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 6

    Organism-specific databases

    HGNCiHGNC:21601. SLC35B3.

    Subcellular locationi

    Golgi apparatus membrane 1 Publication; Multi-pass membrane protein 1 Publication

    GO - Cellular componenti

    1. Golgi membrane Source: Reactome
    2. integral component of membrane Source: UniProtKB-KW
    3. mitochondrion Source: Ensembl

    Keywords - Cellular componenti

    Golgi apparatus, Membrane

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA134889889.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 401401Adenosine 3'-phospho 5'-phosphosulfate transporter 2PRO_0000213379Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi12 – 121N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi71 – 711N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi254 – 2541N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    MaxQBiQ9H1N7.
    PaxDbiQ9H1N7.
    PRIDEiQ9H1N7.

    PTM databases

    PhosphoSiteiQ9H1N7.

    Expressioni

    Tissue specificityi

    Preferentially and highly expressed in colon.1 Publication

    Gene expression databases

    ArrayExpressiQ9H1N7.
    BgeeiQ9H1N7.
    CleanExiHS_SLC35B3.
    GenevestigatoriQ9H1N7.

    Interactioni

    Protein-protein interaction databases

    BioGridi119208. 1 interaction.
    STRINGi9606.ENSP00000368981.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9H1N7.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei78 – 9821HelicalSequence AnalysisAdd
    BLAST
    Transmembranei114 – 13421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei147 – 16721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei170 – 19021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei196 – 21621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei223 – 24321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei267 – 28721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei298 – 31720HelicalSequence AnalysisAdd
    BLAST
    Transmembranei324 – 34623HelicalSequence AnalysisAdd
    BLAST
    Transmembranei349 – 36921HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0697.
    HOGENOMiHOG000006136.
    HOVERGENiHBG066829.
    InParanoidiQ9H1N7.
    KOiK15277.
    OMAiAMKLHNG.
    OrthoDBiEOG7B8S43.
    PhylomeDBiQ9H1N7.
    TreeFamiTF314523.

    Family and domain databases

    InterProiIPR013657. UAA.
    [Graphical view]
    PfamiPF08449. UAA. 1 hit.
    [Graphical view]

    Sequences (3)i

    Sequence statusi: Complete.

    This entry describes 3 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q9H1N7-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MDLTQQAKDI QNITVQETNK NNSESIECSK ITMDLKFNNS RKYISITVPS    50
    KTQTMSPHIK SVDDVVVLGM NLSKFNKLTQ FFICVAGVFV FYLIYGYLQE 100
    LIFSVEGFKS CGWYLTLVQF AFYSIFGLIE LQLIQDKRRR IPGKTYMIIA 150
    FLTVGTMGLS NTSLGYLNYP TQVIFKCCKL IPVMLGGVFI QGKRYNVADV 200
    SAAICMSLGL IWFTLADSTT APNFNLTGVV LISLALCADA VIGNVQEKAM 250
    KLHNASNSEM VLYSYSIGFV YILLGLTCTS GLGPAVTFCA KNPVRTYGYA 300
    FLFSLTGYFG ISFVLALIKI FGALIAVTVT TGRKAMTIVL SFIFFAKPFT 350
    FQYVWSGLLV VLGIFLNVYS KNMDKIRLPS LYDLINKSVE ARKSRTLAQT 400
    V 401
    Length:401
    Mass (Da):44,593
    Last modified:March 1, 2001 - v1
    Checksum:i2582EFD45ECA842C
    GO
    Isoform 2 (identifier: Q9H1N7-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         141-153: IPGKTYMIIAFLT → YVVCFYFLIFHLY
         154-401: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:153
    Mass (Da):17,914
    Checksum:iAEF35780DBBDA8E2
    GO
    Isoform 3 (identifier: Q9H1N7-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         261-283: VLYSYSIGFVYILLGLTCTSGLG → NITTSSYSGVFIFPNKLKIPLVL
         284-401: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:283
    Mass (Da):31,531
    Checksum:i7A9DF4B6ACC02E39
    GO

    Sequence cautioni

    The sequence AAD27728.1 differs from that shown. Reason: Frameshift at several positions.
    The sequence CAE46041.1 differs from that shown. Reason: Erroneous initiation.

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei141 – 15313IPGKT…IAFLT → YVVCFYFLIFHLY in isoform 2. 1 PublicationVSP_016193Add
    BLAST
    Alternative sequencei154 – 401248Missing in isoform 2. 1 PublicationVSP_016194Add
    BLAST
    Alternative sequencei261 – 28323VLYSY…TSGLG → NITTSSYSGVFIFPNKLKIP LVL in isoform 3. 1 PublicationVSP_016195Add
    BLAST
    Alternative sequencei284 – 401118Missing in isoform 3. 1 PublicationVSP_016196Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB231931 mRNA. Translation: BAE93015.1.
    AF132953 mRNA. Translation: AAD27728.1. Frameshift.
    BX538271 mRNA. Translation: CAD98078.1.
    BX641086 mRNA. Translation: CAE46041.1. Different initiation.
    AL355815 Genomic DNA. Translation: CAC19504.1.
    BC006973 mRNA. Translation: AAH06973.1.
    CCDSiCCDS4508.1. [Q9H1N7-1]
    RefSeqiNP_001136013.1. NM_001142541.2. [Q9H1N7-1]
    NP_057032.2. NM_015948.4. [Q9H1N7-1]
    XP_005249214.1. XM_005249157.1. [Q9H1N7-1]
    XP_006715165.1. XM_006715102.1. [Q9H1N7-1]
    UniGeneiHs.285847.

    Genome annotation databases

    EnsembliENST00000379660; ENSP00000368981; ENSG00000124786. [Q9H1N7-1]
    GeneIDi51000.
    KEGGihsa:51000.
    UCSCiuc003myb.3. human. [Q9H1N7-1]

    Polymorphism databases

    DMDMi74752580.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Web resourcesi

    GGDB

    GlycoGene database

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB231931 mRNA. Translation: BAE93015.1 .
    AF132953 mRNA. Translation: AAD27728.1 . Frameshift.
    BX538271 mRNA. Translation: CAD98078.1 .
    BX641086 mRNA. Translation: CAE46041.1 . Different initiation.
    AL355815 Genomic DNA. Translation: CAC19504.1 .
    BC006973 mRNA. Translation: AAH06973.1 .
    CCDSi CCDS4508.1. [Q9H1N7-1 ]
    RefSeqi NP_001136013.1. NM_001142541.2. [Q9H1N7-1 ]
    NP_057032.2. NM_015948.4. [Q9H1N7-1 ]
    XP_005249214.1. XM_005249157.1. [Q9H1N7-1 ]
    XP_006715165.1. XM_006715102.1. [Q9H1N7-1 ]
    UniGenei Hs.285847.

    3D structure databases

    ProteinModelPortali Q9H1N7.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 119208. 1 interaction.
    STRINGi 9606.ENSP00000368981.

    Protein family/group databases

    TCDBi 2.A.7.11.5. the drug/metabolite transporter (dmt) superfamily.

    PTM databases

    PhosphoSitei Q9H1N7.

    Polymorphism databases

    DMDMi 74752580.

    Proteomic databases

    MaxQBi Q9H1N7.
    PaxDbi Q9H1N7.
    PRIDEi Q9H1N7.

    Protocols and materials databases

    DNASUi 51000.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000379660 ; ENSP00000368981 ; ENSG00000124786 . [Q9H1N7-1 ]
    GeneIDi 51000.
    KEGGi hsa:51000.
    UCSCi uc003myb.3. human. [Q9H1N7-1 ]

    Organism-specific databases

    CTDi 51000.
    GeneCardsi GC06M008358.
    HGNCi HGNC:21601. SLC35B3.
    MIMi 610845. gene.
    neXtProti NX_Q9H1N7.
    PharmGKBi PA134889889.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG0697.
    HOGENOMi HOG000006136.
    HOVERGENi HBG066829.
    InParanoidi Q9H1N7.
    KOi K15277.
    OMAi AMKLHNG.
    OrthoDBi EOG7B8S43.
    PhylomeDBi Q9H1N7.
    TreeFami TF314523.

    Enzyme and pathway databases

    Reactomei REACT_22151. Transport of nucleotide sugars.
    REACT_6840. Transport and synthesis of PAPS.

    Miscellaneous databases

    ChiTaRSi SLC35B3. human.
    GenomeRNAii 51000.
    NextBioi 53452.
    PROi Q9H1N7.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q9H1N7.
    Bgeei Q9H1N7.
    CleanExi HS_SLC35B3.
    Genevestigatori Q9H1N7.

    Family and domain databases

    InterProi IPR013657. UAA.
    [Graphical view ]
    Pfami PF08449. UAA. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning and characterization of a novel 3'-phosphoadenosine 5'-phosphosulfate transporter, PAPST2."
      Kamiyama S., Sasaki N., Goda E., Ui-Tei K., Saigo K., Narimatsu H., Jigami Y., Kannagi R., Irimura T., Nishihara S.
      J. Biol. Chem. 281:10945-10953(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
      Tissue: Colon.
    2. "Identification of novel human genes evolutionarily conserved in Caenorhabditis elegans by comparative proteomics."
      Lai C.-H., Chou C.-Y., Ch'ang L.-Y., Liu C.-S., Lin W.-C.
      Genome Res. 10:703-713(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 2-401 (ISOFORM 2).
      Tissue: Endometrial tumor and Liver.
    4. "The DNA sequence and analysis of human chromosome 6."
      Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D.
      , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J., French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S.
      Nature 425:805-811(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Kidney.
    6. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiS35B3_HUMAN
    AccessioniPrimary (citable) accession number: Q9H1N7
    Secondary accession number(s): A6NKX9
    , Q1XH11, Q6MZJ0, Q7Z662, Q9Y308
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 22, 2005
    Last sequence update: March 1, 2001
    Last modified: October 1, 2014
    This is version 103 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 6
      Human chromosome 6: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3