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Protein

Adenosine 3'-phospho 5'-phosphosulfate transporter 2

Gene

SLC35B3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Mediates the transport of adenosine 3'-phospho 5'-phosphosulfate (PAPS), from cytosol into Golgi. PAPS is a universal sulfuryl donor for sulfation events that take place in the Golgi. Compensates for the insufficient expression of SLC35B2/PAPST1 during the synthesis of sulfated glycoconjugates in the colon.1 Publication

Kineticsi

  1. KM=2.2 µM for PAPS1 Publication

GO - Biological processi

  1. 3'-phosphoadenosine 5'-phosphosulfate biosynthetic process Source: Reactome
  2. 3'-phosphoadenosine 5'-phosphosulfate metabolic process Source: Reactome
  3. carbohydrate metabolic process Source: Reactome
  4. glycosaminoglycan metabolic process Source: Reactome
  5. pathogenesis Source: Reactome
  6. regulation of mitochondrion degradation Source: ParkinsonsUK-UCL
  7. small molecule metabolic process Source: Reactome
  8. transmembrane transport Source: Reactome
  9. xenobiotic metabolic process Source: Reactome
Complete GO annotation...

Keywords - Biological processi

Transport

Enzyme and pathway databases

ReactomeiREACT_22151. Transport of nucleotide sugars.
REACT_6840. Transport and synthesis of PAPS.

Protein family/group databases

TCDBi2.A.7.11.5. the drug/metabolite transporter (dmt) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Adenosine 3'-phospho 5'-phosphosulfate transporter 2
Alternative name(s):
3'-phosphoadenosine 5'-phosphosulfate transporter
PAPS transporter 2
Solute carrier family 35 member B3
Gene namesi
Name:SLC35B3
Synonyms:C6orf196, PAPST2
ORF Names:CGI-19
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 6

Organism-specific databases

HGNCiHGNC:21601. SLC35B3.

Subcellular locationi

  1. Golgi apparatus membrane 1 Publication; Multi-pass membrane protein 1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei78 – 9821HelicalSequence AnalysisAdd
BLAST
Transmembranei114 – 13421HelicalSequence AnalysisAdd
BLAST
Transmembranei147 – 16721HelicalSequence AnalysisAdd
BLAST
Transmembranei170 – 19021HelicalSequence AnalysisAdd
BLAST
Transmembranei196 – 21621HelicalSequence AnalysisAdd
BLAST
Transmembranei223 – 24321HelicalSequence AnalysisAdd
BLAST
Transmembranei267 – 28721HelicalSequence AnalysisAdd
BLAST
Transmembranei298 – 31720HelicalSequence AnalysisAdd
BLAST
Transmembranei324 – 34623HelicalSequence AnalysisAdd
BLAST
Transmembranei349 – 36921HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. Golgi apparatus Source: HPA
  2. Golgi membrane Source: Reactome
  3. integral component of membrane Source: UniProtKB-KW
  4. intracellular membrane-bounded organelle Source: HPA
  5. mitochondrion Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134889889.

Polymorphism and mutation databases

DMDMi74752580.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 401401Adenosine 3'-phospho 5'-phosphosulfate transporter 2PRO_0000213379Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi12 – 121N-linked (GlcNAc...)Sequence Analysis
Glycosylationi71 – 711N-linked (GlcNAc...)Sequence Analysis
Glycosylationi254 – 2541N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiQ9H1N7.
PaxDbiQ9H1N7.
PRIDEiQ9H1N7.

PTM databases

PhosphoSiteiQ9H1N7.

Expressioni

Tissue specificityi

Preferentially and highly expressed in colon.1 Publication

Gene expression databases

BgeeiQ9H1N7.
CleanExiHS_SLC35B3.
ExpressionAtlasiQ9H1N7. baseline and differential.
GenevestigatoriQ9H1N7.

Organism-specific databases

HPAiHPA054661.
HPA057801.

Interactioni

Protein-protein interaction databases

BioGridi119208. 1 interaction.
STRINGi9606.ENSP00000368981.

Structurei

3D structure databases

ProteinModelPortaliQ9H1N7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0697.
GeneTreeiENSGT00530000063090.
HOGENOMiHOG000006136.
HOVERGENiHBG066829.
KOiK15277.
OMAiRFNKLTQ.
OrthoDBiEOG7B8S43.
PhylomeDBiQ9H1N7.
TreeFamiTF314523.

Family and domain databases

InterProiIPR013657. UAA.
[Graphical view]
PfamiPF08449. UAA. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9H1N7-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDLTQQAKDI QNITVQETNK NNSESIECSK ITMDLKFNNS RKYISITVPS
60 70 80 90 100
KTQTMSPHIK SVDDVVVLGM NLSKFNKLTQ FFICVAGVFV FYLIYGYLQE
110 120 130 140 150
LIFSVEGFKS CGWYLTLVQF AFYSIFGLIE LQLIQDKRRR IPGKTYMIIA
160 170 180 190 200
FLTVGTMGLS NTSLGYLNYP TQVIFKCCKL IPVMLGGVFI QGKRYNVADV
210 220 230 240 250
SAAICMSLGL IWFTLADSTT APNFNLTGVV LISLALCADA VIGNVQEKAM
260 270 280 290 300
KLHNASNSEM VLYSYSIGFV YILLGLTCTS GLGPAVTFCA KNPVRTYGYA
310 320 330 340 350
FLFSLTGYFG ISFVLALIKI FGALIAVTVT TGRKAMTIVL SFIFFAKPFT
360 370 380 390 400
FQYVWSGLLV VLGIFLNVYS KNMDKIRLPS LYDLINKSVE ARKSRTLAQT

V
Length:401
Mass (Da):44,593
Last modified:March 1, 2001 - v1
Checksum:i2582EFD45ECA842C
GO
Isoform 2 (identifier: Q9H1N7-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     141-153: IPGKTYMIIAFLT → YVVCFYFLIFHLY
     154-401: Missing.

Note: No experimental confirmation available.

Show »
Length:153
Mass (Da):17,914
Checksum:iAEF35780DBBDA8E2
GO
Isoform 3 (identifier: Q9H1N7-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     261-283: VLYSYSIGFVYILLGLTCTSGLG → NITTSSYSGVFIFPNKLKIPLVL
     284-401: Missing.

Note: No experimental confirmation available.

Show »
Length:283
Mass (Da):31,531
Checksum:i7A9DF4B6ACC02E39
GO

Sequence cautioni

The sequence AAD27728.1 differs from that shown. Reason: Frameshift at several positions. Curated
The sequence CAE46041.1 differs from that shown. Reason: Erroneous initiation. Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei141 – 15313IPGKT…IAFLT → YVVCFYFLIFHLY in isoform 2. 1 PublicationVSP_016193Add
BLAST
Alternative sequencei154 – 401248Missing in isoform 2. 1 PublicationVSP_016194Add
BLAST
Alternative sequencei261 – 28323VLYSY…TSGLG → NITTSSYSGVFIFPNKLKIP LVL in isoform 3. 1 PublicationVSP_016195Add
BLAST
Alternative sequencei284 – 401118Missing in isoform 3. 1 PublicationVSP_016196Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB231931 mRNA. Translation: BAE93015.1.
AF132953 mRNA. Translation: AAD27728.1. Frameshift.
BX538271 mRNA. Translation: CAD98078.1.
BX641086 mRNA. Translation: CAE46041.1. Different initiation.
AL355815 Genomic DNA. Translation: CAC19504.1.
BC006973 mRNA. Translation: AAH06973.1.
CCDSiCCDS4508.1. [Q9H1N7-1]
RefSeqiNP_001136013.1. NM_001142541.2. [Q9H1N7-1]
NP_057032.2. NM_015948.4. [Q9H1N7-1]
XP_005249214.1. XM_005249157.1. [Q9H1N7-1]
XP_006715165.1. XM_006715102.2. [Q9H1N7-1]
UniGeneiHs.285847.

Genome annotation databases

EnsembliENST00000379660; ENSP00000368981; ENSG00000124786. [Q9H1N7-1]
GeneIDi51000.
KEGGihsa:51000.
UCSCiuc003myb.3. human. [Q9H1N7-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Web resourcesi

GGDB

GlycoGene database

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB231931 mRNA. Translation: BAE93015.1.
AF132953 mRNA. Translation: AAD27728.1. Frameshift.
BX538271 mRNA. Translation: CAD98078.1.
BX641086 mRNA. Translation: CAE46041.1. Different initiation.
AL355815 Genomic DNA. Translation: CAC19504.1.
BC006973 mRNA. Translation: AAH06973.1.
CCDSiCCDS4508.1. [Q9H1N7-1]
RefSeqiNP_001136013.1. NM_001142541.2. [Q9H1N7-1]
NP_057032.2. NM_015948.4. [Q9H1N7-1]
XP_005249214.1. XM_005249157.1. [Q9H1N7-1]
XP_006715165.1. XM_006715102.2. [Q9H1N7-1]
UniGeneiHs.285847.

3D structure databases

ProteinModelPortaliQ9H1N7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi119208. 1 interaction.
STRINGi9606.ENSP00000368981.

Protein family/group databases

TCDBi2.A.7.11.5. the drug/metabolite transporter (dmt) superfamily.

PTM databases

PhosphoSiteiQ9H1N7.

Polymorphism and mutation databases

DMDMi74752580.

Proteomic databases

MaxQBiQ9H1N7.
PaxDbiQ9H1N7.
PRIDEiQ9H1N7.

Protocols and materials databases

DNASUi51000.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000379660; ENSP00000368981; ENSG00000124786. [Q9H1N7-1]
GeneIDi51000.
KEGGihsa:51000.
UCSCiuc003myb.3. human. [Q9H1N7-1]

Organism-specific databases

CTDi51000.
GeneCardsiGC06M008358.
HGNCiHGNC:21601. SLC35B3.
HPAiHPA054661.
HPA057801.
MIMi610845. gene.
neXtProtiNX_Q9H1N7.
PharmGKBiPA134889889.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiCOG0697.
GeneTreeiENSGT00530000063090.
HOGENOMiHOG000006136.
HOVERGENiHBG066829.
KOiK15277.
OMAiRFNKLTQ.
OrthoDBiEOG7B8S43.
PhylomeDBiQ9H1N7.
TreeFamiTF314523.

Enzyme and pathway databases

ReactomeiREACT_22151. Transport of nucleotide sugars.
REACT_6840. Transport and synthesis of PAPS.

Miscellaneous databases

ChiTaRSiSLC35B3. human.
GenomeRNAii51000.
NextBioi53452.
PROiQ9H1N7.
SOURCEiSearch...

Gene expression databases

BgeeiQ9H1N7.
CleanExiHS_SLC35B3.
ExpressionAtlasiQ9H1N7. baseline and differential.
GenevestigatoriQ9H1N7.

Family and domain databases

InterProiIPR013657. UAA.
[Graphical view]
PfamiPF08449. UAA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning and characterization of a novel 3'-phosphoadenosine 5'-phosphosulfate transporter, PAPST2."
    Kamiyama S., Sasaki N., Goda E., Ui-Tei K., Saigo K., Narimatsu H., Jigami Y., Kannagi R., Irimura T., Nishihara S.
    J. Biol. Chem. 281:10945-10953(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    Tissue: Colon.
  2. "Identification of novel human genes evolutionarily conserved in Caenorhabditis elegans by comparative proteomics."
    Lai C.-H., Chou C.-Y., Ch'ang L.-Y., Liu C.-S., Lin W.-C.
    Genome Res. 10:703-713(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 2-401 (ISOFORM 2).
    Tissue: Endometrial tumor and Liver.
  4. "The DNA sequence and analysis of human chromosome 6."
    Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D.
    , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J., French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S.
    Nature 425:805-811(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Kidney.

Entry informationi

Entry nameiS35B3_HUMAN
AccessioniPrimary (citable) accession number: Q9H1N7
Secondary accession number(s): A6NKX9
, Q1XH11, Q6MZJ0, Q7Z662, Q9Y308
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 22, 2005
Last sequence update: March 1, 2001
Last modified: April 29, 2015
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 6
    Human chromosome 6: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.