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Q9H1N7 (S35B3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 101. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Adenosine 3'-phospho 5'-phosphosulfate transporter 2
Alternative name(s):
3'-phosphoadenosine 5'-phosphosulfate transporter
PAPS transporter 2
Solute carrier family 35 member B3
Gene names
Name:SLC35B3
Synonyms:C6orf196, PAPST2
ORF Names:CGI-19
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length401 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Mediates the transport of adenosine 3'-phospho 5'-phosphosulfate (PAPS), from cytosol into Golgi. PAPS is a universal sulfuryl donor for sulfation events that take place in the Golgi. Compensates for the insufficient expression of SLC35B2/PAPST1 during the synthesis of sulfated glycoconjugates in the colon. Ref.1

Subcellular location

Golgi apparatus membrane; Multi-pass membrane protein Ref.1.

Tissue specificity

Preferentially and highly expressed in colon. Ref.1

Sequence similarities

Belongs to the nucleotide-sugar transporter family. SLC35B subfamily.

Biophysicochemical properties

Kinetic parameters:

KM=2.2 µM for PAPS Ref.1

Sequence caution

The sequence AAD27728.1 differs from that shown. Reason: Frameshift at several positions.

The sequence CAE46041.1 differs from that shown. Reason: Erroneous initiation.

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9H1N7-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9H1N7-2)

The sequence of this isoform differs from the canonical sequence as follows:
     141-153: IPGKTYMIIAFLT → YVVCFYFLIFHLY
     154-401: Missing.
Note: No experimental confirmation available.
Isoform 3 (identifier: Q9H1N7-3)

The sequence of this isoform differs from the canonical sequence as follows:
     261-283: VLYSYSIGFVYILLGLTCTSGLG → NITTSSYSGVFIFPNKLKIPLVL
     284-401: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 401401Adenosine 3'-phospho 5'-phosphosulfate transporter 2
PRO_0000213379

Regions

Transmembrane78 – 9821Helical; Potential
Transmembrane114 – 13421Helical; Potential
Transmembrane147 – 16721Helical; Potential
Transmembrane170 – 19021Helical; Potential
Transmembrane196 – 21621Helical; Potential
Transmembrane223 – 24321Helical; Potential
Transmembrane267 – 28721Helical; Potential
Transmembrane298 – 31720Helical; Potential
Transmembrane324 – 34623Helical; Potential
Transmembrane349 – 36921Helical; Potential

Amino acid modifications

Glycosylation121N-linked (GlcNAc...) Potential
Glycosylation711N-linked (GlcNAc...) Potential
Glycosylation2541N-linked (GlcNAc...) Potential

Natural variations

Alternative sequence141 – 15313IPGKT…IAFLT → YVVCFYFLIFHLY in isoform 2.
VSP_016193
Alternative sequence154 – 401248Missing in isoform 2.
VSP_016194
Alternative sequence261 – 28323VLYSY…TSGLG → NITTSSYSGVFIFPNKLKIP LVL in isoform 3.
VSP_016195
Alternative sequence284 – 401118Missing in isoform 3.
VSP_016196

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified March 1, 2001. Version 1.
Checksum: 2582EFD45ECA842C

FASTA40144,593
        10         20         30         40         50         60 
MDLTQQAKDI QNITVQETNK NNSESIECSK ITMDLKFNNS RKYISITVPS KTQTMSPHIK 

        70         80         90        100        110        120 
SVDDVVVLGM NLSKFNKLTQ FFICVAGVFV FYLIYGYLQE LIFSVEGFKS CGWYLTLVQF 

       130        140        150        160        170        180 
AFYSIFGLIE LQLIQDKRRR IPGKTYMIIA FLTVGTMGLS NTSLGYLNYP TQVIFKCCKL 

       190        200        210        220        230        240 
IPVMLGGVFI QGKRYNVADV SAAICMSLGL IWFTLADSTT APNFNLTGVV LISLALCADA 

       250        260        270        280        290        300 
VIGNVQEKAM KLHNASNSEM VLYSYSIGFV YILLGLTCTS GLGPAVTFCA KNPVRTYGYA 

       310        320        330        340        350        360 
FLFSLTGYFG ISFVLALIKI FGALIAVTVT TGRKAMTIVL SFIFFAKPFT FQYVWSGLLV 

       370        380        390        400 
VLGIFLNVYS KNMDKIRLPS LYDLINKSVE ARKSRTLAQT V 

« Hide

Isoform 2 [UniParc].

Checksum: AEF35780DBBDA8E2
Show »

FASTA15317,914
Isoform 3 [UniParc].

Checksum: 7A9DF4B6ACC02E39
Show »

FASTA28331,531

References

« Hide 'large scale' references
[1]"Molecular cloning and characterization of a novel 3'-phosphoadenosine 5'-phosphosulfate transporter, PAPST2."
Kamiyama S., Sasaki N., Goda E., Ui-Tei K., Saigo K., Narimatsu H., Jigami Y., Kannagi R., Irimura T., Nishihara S.
J. Biol. Chem. 281:10945-10953(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
Tissue: Colon.
[2]"Identification of novel human genes evolutionarily conserved in Caenorhabditis elegans by comparative proteomics."
Lai C.-H., Chou C.-Y., Ch'ang L.-Y., Liu C.-S., Lin W.-C.
Genome Res. 10:703-713(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[3]"The full-ORF clone resource of the German cDNA consortium."
Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I.
BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 2-401 (ISOFORM 2).
Tissue: Endometrial tumor and Liver.
[4]"The DNA sequence and analysis of human chromosome 6."
Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D. expand/collapse author list , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J., French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S.
Nature 425:805-811(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Kidney.
[6]"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A., Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Web resources

GGDB

GlycoGene database

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB231931 mRNA. Translation: BAE93015.1.
AF132953 mRNA. Translation: AAD27728.1. Frameshift.
BX538271 mRNA. Translation: CAD98078.1.
BX641086 mRNA. Translation: CAE46041.1. Different initiation.
AL355815 Genomic DNA. Translation: CAC19504.1.
BC006973 mRNA. Translation: AAH06973.1.
CCDSCCDS4508.1. [Q9H1N7-1]
RefSeqNP_001136013.1. NM_001142541.2. [Q9H1N7-1]
NP_057032.2. NM_015948.4. [Q9H1N7-1]
XP_005249214.1. XM_005249157.1. [Q9H1N7-1]
XP_006715165.1. XM_006715102.1. [Q9H1N7-1]
UniGeneHs.285847.

3D structure databases

ProteinModelPortalQ9H1N7.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid119208. 1 interaction.
STRING9606.ENSP00000368981.

Protein family/group databases

TCDB2.A.7.11.5. the drug/metabolite transporter (dmt) superfamily.

PTM databases

PhosphoSiteQ9H1N7.

Polymorphism databases

DMDM74752580.

Proteomic databases

MaxQBQ9H1N7.
PaxDbQ9H1N7.
PRIDEQ9H1N7.

Protocols and materials databases

DNASU51000.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000339306; ENSP00000345902; ENSG00000124786. [Q9H1N7-3]
ENST00000379660; ENSP00000368981; ENSG00000124786. [Q9H1N7-1]
GeneID51000.
KEGGhsa:51000.
UCSCuc003myb.3. human. [Q9H1N7-1]

Organism-specific databases

CTD51000.
GeneCardsGC06M008358.
HGNCHGNC:21601. SLC35B3.
MIM610845. gene.
neXtProtNX_Q9H1N7.
PharmGKBPA134889889.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG0697.
HOGENOMHOG000006136.
HOVERGENHBG066829.
InParanoidQ9H1N7.
KOK15277.
OMAAMKLHNG.
OrthoDBEOG7B8S43.
PhylomeDBQ9H1N7.
TreeFamTF314523.

Enzyme and pathway databases

ReactomeREACT_111217. Metabolism.
REACT_116125. Disease.
REACT_15518. Transmembrane transport of small molecules.

Gene expression databases

ArrayExpressQ9H1N7.
BgeeQ9H1N7.
CleanExHS_SLC35B3.
GenevestigatorQ9H1N7.

Family and domain databases

InterProIPR013657. UAA.
[Graphical view]
PfamPF08449. UAA. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSSLC35B3. human.
GenomeRNAi51000.
NextBio53452.
PROQ9H1N7.
SOURCESearch...

Entry information

Entry nameS35B3_HUMAN
AccessionPrimary (citable) accession number: Q9H1N7
Secondary accession number(s): A6NKX9 expand/collapse secondary AC list , Q1XH11, Q6MZJ0, Q7Z662, Q9Y308
Entry history
Integrated into UniProtKB/Swiss-Prot: November 22, 2005
Last sequence update: March 1, 2001
Last modified: July 9, 2014
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 6

Human chromosome 6: entries, gene names and cross-references to MIM