Q9H1N7 (S35B3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Adenosine 3'-phospho 5'-phosphosulfate transporter 2

Alternative name(s):
3'-phosphoadenosine 5'-phosphosulfate transporter
PAPS transporter 2
Solute carrier family 35 member B3

Gene names

Name:	SLC35B3
Synonyms:	C6orf196, PAPST2
ORF Names:	CGI-19

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	401 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Mediates the transport of adenosine 3'-phospho 5'-phosphosulfate (PAPS), from cytosol into Golgi. PAPS is a universal sulfuryl donor for sulfation events that take place in the Golgi. Compensates for the insufficient expression of SLC35B2/PAPST1 during the synthesis of sulfated glycoconjugates in the colon. Ref.1
Subcellular location	Golgi apparatus membrane; Multi-pass membrane protein Ref.1.
Tissue specificity	Preferentially and highly expressed in colon. Ref.1
Sequence similarities	Belongs to the nucleotide-sugar transporter family. SLC35B subfamily.
Biophysicochemical properties	Kinetic parameters: KM=2.2 µM for PAPS Ref.1
Sequence caution	The sequence AAD27728.1 differs from that shown. Reason: Frameshift at several positions. The sequence CAE46041.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Transport
Cellular component	Golgi apparatus Membrane
Coding sequence diversity	Alternative splicing
Domain	Transmembrane Transmembrane helix
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	transmembrane transport Inferred from electronic annotation. Source: InterPro
Cellular_component	Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW mitochondrion Inferred from electronic annotation. Source: Compara
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9H1N7-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9H1N7-2)

The sequence of this isoform differs from the canonical sequence as follows:
     141-153: IPGKTYMIIAFLT → YVVCFYFLIFHLY
     154-401: Missing.

Note: No experimental confirmation available.

Isoform 3 (identifier: Q9H1N7-3)

The sequence of this isoform differs from the canonical sequence as follows:
     261-283: VLYSYSIGFVYILLGLTCTSGLG → NITTSSYSGVFIFPNKLKIPLVL
     284-401: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 401	401	Adenosine 3'-phospho 5'-phosphosulfate transporter 2		PRO_0000213379
Regions
	Transmembrane	78 – 98	21	Helical; Potential
	Transmembrane	114 – 134	21	Helical; Potential
	Transmembrane	147 – 167	21	Helical; Potential
	Transmembrane	170 – 190	21	Helical; Potential
	Transmembrane	196 – 216	21	Helical; Potential
	Transmembrane	223 – 243	21	Helical; Potential
	Transmembrane	267 – 287	21	Helical; Potential
	Transmembrane	298 – 317	20	Helical; Potential
	Transmembrane	324 – 346	23	Helical; Potential
	Transmembrane	349 – 369	21	Helical; Potential
Amino acid modifications
	Glycosylation	12	1	N-linked (GlcNAc...) Potential
	Glycosylation	71	1	N-linked (GlcNAc...) Potential
	Glycosylation	254	1	N-linked (GlcNAc...) Potential
Natural variations
	Alternative sequence	141 – 153	13	IPGKT…IAFLT → YVVCFYFLIFHLY in isoform 2.		VSP_016193
	Alternative sequence	154 – 401	248	Missing in isoform 2.		VSP_016194
	Alternative sequence	261 – 283	23	VLYSY…TSGLG → NITTSSYSGVFIFPNKLKIP LVL in isoform 3.		VSP_016195
	Alternative sequence	284 – 401	118	Missing in isoform 3.		VSP_016196

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: 2582EFD45ECA842C	FASTA	401	44,593
	10 20 30 40 50 60 MDLTQQAKDI QNITVQETNK NNSESIECSK ITMDLKFNNS RKYISITVPS KTQTMSPHIK 70 80 90 100 110 120 SVDDVVVLGM NLSKFNKLTQ FFICVAGVFV FYLIYGYLQE LIFSVEGFKS CGWYLTLVQF 130 140 150 160 170 180 AFYSIFGLIE LQLIQDKRRR IPGKTYMIIA FLTVGTMGLS NTSLGYLNYP TQVIFKCCKL 190 200 210 220 230 240 IPVMLGGVFI QGKRYNVADV SAAICMSLGL IWFTLADSTT APNFNLTGVV LISLALCADA 250 260 270 280 290 300 VIGNVQEKAM KLHNASNSEM VLYSYSIGFV YILLGLTCTS GLGPAVTFCA KNPVRTYGYA 310 320 330 340 350 360 FLFSLTGYFG ISFVLALIKI FGALIAVTVT TGRKAMTIVL SFIFFAKPFT FQYVWSGLLV 370 380 390 400 VLGIFLNVYS KNMDKIRLPS LYDLINKSVE ARKSRTLAQT V
	Isoform 2 [UniParc]. Checksum: AEF35780DBBDA8E2	FASTA	153	17,914
	10 20 30 40 50 60 MDLTQQAKDI QNITVQETNK NNSESIECSK ITMDLKFNNS RKYISITVPS KTQTMSPHIK 70 80 90 100 110 120 SVDDVVVLGM NLSKFNKLTQ FFICVAGVFV FYLIYGYLQE LIFSVEGFKS CGWYLTLVQF 130 140 150 AFYSIFGLIE LQLIQDKRRR YVVCFYFLIF HLY
	Isoform 3 [UniParc]. Checksum: 7A9DF4B6ACC02E39	FASTA	283	31,531
	10 20 30 40 50 60 MDLTQQAKDI QNITVQETNK NNSESIECSK ITMDLKFNNS RKYISITVPS KTQTMSPHIK 70 80 90 100 110 120 SVDDVVVLGM NLSKFNKLTQ FFICVAGVFV FYLIYGYLQE LIFSVEGFKS CGWYLTLVQF 130 140 150 160 170 180 AFYSIFGLIE LQLIQDKRRR IPGKTYMIIA FLTVGTMGLS NTSLGYLNYP TQVIFKCCKL 190 200 210 220 230 240 IPVMLGGVFI QGKRYNVADV SAAICMSLGL IWFTLADSTT APNFNLTGVV LISLALCADA 250 260 270 280 VIGNVQEKAM KLHNASNSEM NITTSSYSGV FIFPNKLKIP LVL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning and characterization of a novel 3'-phosphoadenosine 5'-phosphosulfate transporter, PAPST2." Kamiyama S., Sasaki N., Goda E., Ui-Tei K., Saigo K., Narimatsu H., Jigami Y., Kannagi R., Irimura T., Nishihara S. J. Biol. Chem. 281:10945-10953(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION, TISSUE SPECIFICITY. Tissue: Colon.
[2]	"Identification of novel human genes evolutionarily conserved in Caenorhabditis elegans by comparative proteomics." Lai C.-H., Chou C.-Y., Ch'ang L.-Y., Liu C.-S., Lin W.-C. Genome Res. 10:703-713(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[3]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 2-401 (ISOFORM 2). Tissue: Endometrial tumor and Liver.
[4]	"The DNA sequence and analysis of human chromosome 6." Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D. , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J., French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S. Nature 425:805-811(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Kidney.
+	Additional computationally mapped references.

Web resources

GGDB

GlycoGene database

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB231931 mRNA. Translation: BAE93015.1. AF132953 mRNA. Translation: AAD27728.1. Frameshift. BX538271 mRNA. Translation: CAD98078.1. BX641086 mRNA. Translation: CAE46041.1. Different initiation. AL355815 Genomic DNA. Translation: CAC19504.1. BC006973 mRNA. Translation: AAH06973.1.
IPI	IPI00152951. IPI00385333. IPI00425935.
RefSeq	NP_001136012.1. NM_001142540.1. NP_001136013.1. NM_001142541.1. NP_057032.2. NM_015948.3.
UniGene	Hs.285847.
3D structure databases
ProteinModelPortal	Q9H1N7.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000368981.
Protein family/group databases
TCDB	2.A.7.11.5. drug/metabolite transporter (DMT) superfamily.
PTM databases
PhosphoSite	Q9H1N7.
Polymorphism databases
DMDM	74752580.
Proteomic databases
PaxDb	Q9H1N7.
PRIDE	Q9H1N7.
Protocols and materials databases
DNASU	51000.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000339306; ENSP00000345902; ENSG00000124786. ENST00000379660; ENSP00000368981; ENSG00000124786.
GeneID	51000.
KEGG	hsa:51000.
UCSC	uc003myb.3. human.
Organism-specific databases
CTD	51000.
GeneCards	GC06M008358.
HGNC	HGNC:21601. SLC35B3.
MIM	610845. gene.
neXtProt	NX_Q9H1N7.
PharmGKB	PA134889889.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG0697.
HOGENOM	HOG000006136.
HOVERGEN	HBG066829.
InParanoid	Q9H1N7.
KO	K15277.
OMA	PVRTYGY.
OrthoDB	EOG4PG612.
PhylomeDB	Q9H1N7.
Gene expression databases
ArrayExpress	Q9H1N7.
Bgee	Q9H1N7.
CleanEx	HS_SLC35B3.
Genevestigator	Q9H1N7.
GermOnline	ENSG00000124786. Homo sapiens.
Family and domain databases
InterPro	IPR013657. UAA. [Graphical view]
Pfam	PF08449. UAA. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	SLC35B3. human.
GenomeRNAi	51000.
NextBio	53452.
SOURCE	Search...

Entry information

Entry name

S35B3_HUMAN

Accession

Primary (citable) accession number: Q9H1N7
Secondary accession number(s): A6NKX9 , Q1XH11, Q6MZJ0, Q7Z662, Q9Y308

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 22, 2005
Last sequence update:	March 1, 2001
Last modified:	April 3, 2013
This is version 90 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.