Q9H1K0 (RBNS5_HUMAN) Reviewed, UniProtKB/Swiss-Prot
Last modified
January 25, 2012.
Version 94.
History...
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Rabenosyn-5 Alternative name(s): 110 kDa protein FYVE finger-containing Rab5 effector protein rabenosyn-5 Zinc finger FYVE domain-containing protein 20 | ||
| Gene names |
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| Organism | Homo sapiens (Human) | ||
| Taxonomic identifier | 9606 [NCBI] | ||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Protein attributes
| Sequence length | 784 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level |
General annotation (Comments)
| Function | Rab4/Rab5 effector protein acting in early endocytic membrane fusion and membrane trafficking of recycling endosomes. Required for endosome fusion either homotypically or with clathrin coated vesicles. Plays a role in the lysosomal trafficking of CTSD/cathepsin D from the Golgi to lysosomes. Also promotes the recycling of transferrin directly from early endosomes to the plasma membrane. Binds phospholipid vesicles containing phosphatidylinositol 3-phosphate (PtdInsP3). Ref.1 Ref.5 Ref.6 |
| Subunit structure | Interacts with EHD1, RAB4A, RAB5A, RAB14, RAB22A, RAB24 and VPS45. Binds simultaneously to RAB4A and RAB5A in vitro. Interacts with RAB4A and RAB5A that has been activated by GTP binding. Ref.1 Ref.5 Ref.6 Ref.10 |
| Subcellular location | Cell membrane; Lipid-anchor; Cytoplasmic side. Early endosome membrane; Lipid-anchor Ref.1 Ref.5 Ref.6. |
| Sequence similarities | Contains 1 C2H2-type zinc finger. Contains 1 FYVE-type zinc finger. Contains 1 UIM (ubiquitin-interacting motif) repeat. |
| Sequence caution | The sequence BAC03860.1 differs from that shown. Reason: Erroneous initiation. The sequence BAD92910.1 differs from that shown. Reason: Erroneous initiation. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Protein transport Transport |
| Cellular component | Cell membrane Endosome Membrane |
| Coding sequence diversity | Polymorphism |
| Domain | Coiled coil Zinc-finger |
| Ligand | Metal-binding Zinc |
| PTM | Acetylation Lipoprotein Phosphoprotein |
| Technical term | 3D-structure Complete proteome Reference proteome |
| Gene Ontology (GO) | |
| Biological process | blood coagulation Traceable author statement. Source: Reactome endosome transportNon-traceable author statement Ref.1. Source: UniProtKB protein transportInferred from electronic annotation. Source: UniProtKB-KW |
| Cellular component | early endosome membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membraneInferred from electronic annotation. Source: UniProtKB-SubCell |
| Molecular function | protein binding Inferred from physical interaction Ref.6Ref.10. Source: UniProtKB zinc ion bindingNon-traceable author statement Ref.1. Source: UniProtKB |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||||||||||
Molecule processing | |||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Initiator methionine | 1 | 1 | Removed | ||||||||||||||
| Chain | 2 – 784 | 783 | Rabenosyn-5 | PRO_0000098711 | |||||||||||||
Regions | |||||||||||||||||
| Repeat | 496 – 515 | 20 | UIM | ||||||||||||||
| Zinc finger | 14 – 37 | 24 | C2H2-type | ||||||||||||||
| Zinc finger | 157 – 260 | 104 | FYVE-type | ||||||||||||||
| Region | 100 – 263 | 164 | Necessary for the correct targeting to endosomes | ||||||||||||||
| Region | 264 – 784 | 521 | Necessary for the interaction with EHD1 | ||||||||||||||
| Region | 264 – 500 | 237 | Necessary for the interaction with RAB4A | ||||||||||||||
| Region | 627 – 784 | 158 | Necessary for the interaction with RAB5A | ||||||||||||||
| Coiled coil | 378 – 414 | 37 | Potential | ||||||||||||||
| Coiled coil | 472 – 531 | 60 | Potential | ||||||||||||||
| Compositional bias | 204 – 239 | 36 | Ser-rich | ||||||||||||||
| Compositional bias | 665 – 672 | 8 | Poly-Glu | ||||||||||||||
Amino acid modifications | |||||||||||||||||
| Modified residue | 2 | 1 | N-acetylalanine Ref.9 | ||||||||||||||
| Modified residue | 214 | 1 | Phosphothreonine Ref.8 | ||||||||||||||
| Modified residue | 215 | 1 | Phosphoserine Ref.8 | ||||||||||||||
| Modified residue | 217 | 1 | Phosphoserine Ref.8 | ||||||||||||||
| Modified residue | 601 | 1 | Phosphoserine Ref.7 | ||||||||||||||
Natural variations | |||||||||||||||||
| Natural variant | 591 | 1 | L → P. Corresponds to variant rs9868848 [ dbSNP | Ensembl ]. | VAR_052982 | |||||||||||||
| Natural variant | 641 | 1 | T → A. Corresponds to variant rs9851219 [ dbSNP | Ensembl ]. | VAR_052983 | |||||||||||||
| Natural variant | 722 | 1 | M → I. Corresponds to variant rs9830744 [ dbSNP | Ensembl ]. | VAR_052984 | |||||||||||||
Experimental info | |||||||||||||||||
| Mutagenesis | 626 – 628 | 3 | NPF → APA: Reduces the interaction with EHD1. Abolishes the interaction with EHD1; when associated with 662-APA-664. Ref.6 | ||||||||||||||
| Mutagenesis | 662 – 664 | 3 | NPF → APA: Reduces the interaction with EHD1. Abolishes the interaction with EHD1; when associated with 626-APA-628. Ref.6 | ||||||||||||||
| Sequence conflict | 106 | 1 | F → L in AAG33246. Ref.1 | ||||||||||||||
| Sequence conflict | 720 | 1 | F → L in AAG33246. Ref.1 | ||||||||||||||
Secondary structure | |||||||||||||||||
Helix Strand Turn | |||||||||||||||||
| Helix | 458 – 475 | 18 | |||||||||||||||
| Helix | 479 – 500 | 22 | |||||||||||||||
| Helix | 736 – 755 | 20 | |||||||||||||||
| Helix | 759 – 779 | 21 | |||||||||||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Rabenosyn-5, a novel Rab5 effector, is complexed with hVPS45 and recruited to endosomes through a FYVE finger domain." Nielsen E., Christoforidis S., Uttenweiler-Joseph S., Miaczynska M., Dewitte F., Wilm M., Hoflack B., Zerial M. J. Cell Biol. 151:601-612(2000) [PubMed: 11062261] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION IN ENDOSOMAL RECYCLING, INTERACTION WITH PTDINSP3; GTP-BOUND RAB5A AND VPS45A, SUBCELLULAR LOCATION. Tissue: Cervix carcinoma. |
| [2] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. |
| [3] | Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S., Ohara O., Nagase T., Kikuno R.F. Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-755. Tissue: Brain. |
| [4] | "Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. Sugano S.Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 501-784. Tissue: Brain. |
| [5] | "Divalent Rab effectors regulate the sub-compartmental organization and sorting of early endosomes." de Renzis S., Soennichsen B., Zerial M. Nat. Cell Biol. 4:124-133(2002) [PubMed: 11788822] [Abstract] Cited for: FUNCTION IN ENDOSOMAL RECYCLING, INTERACTION WITH RAB4A AND RAB5A, SUBCELLULAR LOCATION. |
| [6] | "Rabenosyn-5 and EHD1 interact and sequentially regulate protein recycling to the plasma membrane." Naslavsky N., Boehm M., Backlund P.S. Jr., Caplan S. Mol. Biol. Cell 15:2410-2422(2004) [PubMed: 15020713] [Abstract] Cited for: FUNCTION IN ENDOSOMAL RECYCLING, INTERACTION WITH EHD1, MUTAGENESIS OF 626-ASN--PHE-628 AND 662-ASN--PHE-664, SUBCELLULAR LOCATION. |
| [7] | "A probability-based approach for high-throughput protein phosphorylation analysis and site localization." Beausoleil S.A., Villen J., Gerber S.A., Rush J., Gygi S.P. Nat. Biotechnol. 24:1285-1292(2006) [PubMed: 16964243] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-601, MASS SPECTROMETRY. Tissue: Cervix carcinoma. |
| [8] | "Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis." Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D., Yates J.R. III J. Proteome Res. 7:1346-1351(2008) [PubMed: 18220336] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-214; SER-215 AND SER-217, MASS SPECTROMETRY. Tissue: Cervix carcinoma. |
| [9] | "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach." Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S. Anal. Chem. 81:4493-4501(2009) [PubMed: 19413330] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, MASS SPECTROMETRY. Tissue: Embryonic kidney. |
| [10] | "Structural basis of family-wide Rab GTPase recognition by rabenosyn-5." Eathiraj S., Pan X., Ritacco C., Lambright D.G. Nature 436:415-419(2005) [PubMed: 16034420] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.32 ANGSTROMS) OF 458-503 AND 728-784 IN COMPLEX WITH RAB22A, INTERACTION WITH RAB4A; RAB5A; RAB14; RAB22A AND RAB24. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| EMBL GenBank DDBJ | AY009133 mRNA. Translation: AAG33246.1. BC106940 mRNA. Translation: AAI06941.1. AB209673 mRNA. Translation: BAD92910.1. Different initiation. AK092312 mRNA. Translation: BAC03860.1. Different initiation. | ||||||||||||||||||||||||
| IPI | IPI00007057. | ||||||||||||||||||||||||
| RefSeq | NP_071735.2. NM_022340.2. | ||||||||||||||||||||||||
| UniGene | Hs.475565. Hs.706478. | ||||||||||||||||||||||||
3D structure databases | |||||||||||||||||||||||||
| PDBe RCSB PDB PDBj |
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| ProteinModelPortal | Q9H1K0. | ||||||||||||||||||||||||
| SMR | Q9H1K0. Positions 11-38, 100-264, 441-501, 734-784. | ||||||||||||||||||||||||
| ModBase | Search... | ||||||||||||||||||||||||
Protein-protein interaction databases | |||||||||||||||||||||||||
| IntAct | Q9H1K0. 2 interactions. | ||||||||||||||||||||||||
| STRING | Q9H1K0. | ||||||||||||||||||||||||
PTM databases | |||||||||||||||||||||||||
| PhosphoSite | Q9H1K0. | ||||||||||||||||||||||||
Polymorphism databases | |||||||||||||||||||||||||
| DMDM | 108935884. | ||||||||||||||||||||||||
Proteomic databases | |||||||||||||||||||||||||
| PRIDE | Q9H1K0. | ||||||||||||||||||||||||
Protocols and materials databases | |||||||||||||||||||||||||
| StructuralBiologyKnowledgebase | Search... | ||||||||||||||||||||||||
Genome annotation databases | |||||||||||||||||||||||||
| Ensembl | ENST00000253699; ENSP00000253699; ENSG00000131381. | ||||||||||||||||||||||||
| GeneID | 64145. | ||||||||||||||||||||||||
| KEGG | hsa:64145. | ||||||||||||||||||||||||
| NMPDR | fig|9606.3.peg.22178. | ||||||||||||||||||||||||
| UCSC | uc003bzm.1. human. | ||||||||||||||||||||||||
Organism-specific databases | |||||||||||||||||||||||||
| CTD | 64145. | ||||||||||||||||||||||||
| GeneCards | GC03M015090. | ||||||||||||||||||||||||
| H-InvDB | HIX0003097. | ||||||||||||||||||||||||
| HGNC | HGNC:20759. ZFYVE20. | ||||||||||||||||||||||||
| HPA | HPA044878. | ||||||||||||||||||||||||
| MIM | 609511. gene. | ||||||||||||||||||||||||
| neXtProt | NX_Q9H1K0. | ||||||||||||||||||||||||
| PharmGKB | PA134959491. | ||||||||||||||||||||||||
| GenAtlas | Search... | ||||||||||||||||||||||||
Phylogenomic databases | |||||||||||||||||||||||||
| eggNOG | prNOG18274. | ||||||||||||||||||||||||
| GeneTree | ENSGT00390000007159. | ||||||||||||||||||||||||
| HOGENOM | HBG716169. | ||||||||||||||||||||||||
| HOVERGEN | HBG067237. | ||||||||||||||||||||||||
| InParanoid | Q9H1K0. | ||||||||||||||||||||||||
| OMA | YEKLRLC. | ||||||||||||||||||||||||
| OrthoDB | EOG4G1MG0. | ||||||||||||||||||||||||
| PhylomeDB | Q9H1K0. | ||||||||||||||||||||||||
Enzyme and pathway databases | |||||||||||||||||||||||||
| Reactome | REACT_604. Hemostasis. | ||||||||||||||||||||||||
Gene expression databases | |||||||||||||||||||||||||
| ArrayExpress | Q9H1K0. | ||||||||||||||||||||||||
| Bgee | Q9H1K0. | ||||||||||||||||||||||||
| CleanEx | HS_ZFYVE20. | ||||||||||||||||||||||||
| Genevestigator | Q9H1K0. | ||||||||||||||||||||||||
| GermOnline | ENSG00000131381. Homo sapiens. | ||||||||||||||||||||||||
Family and domain databases | |||||||||||||||||||||||||
| InterPro | IPR021565. Rbsn. IPR007087. Znf_C2H2. IPR015880. Znf_C2H2-like. IPR000306. Znf_FYVE. IPR017455. Znf_FYVE-rel. IPR011011. Znf_FYVE_PHD. IPR013083. Znf_RING/FYVE/PHD. [Graphical view] | ||||||||||||||||||||||||
| Gene3D | G3DSA:3.30.40.10. Znf_RING/FYVE/PHD. 2 hits. | ||||||||||||||||||||||||
| KO | K12481. | ||||||||||||||||||||||||
| Pfam | PF01363. FYVE. 1 hit. PF11464. Rbsn. 2 hits. [Graphical view] | ||||||||||||||||||||||||
| SMART | SM00064. FYVE. 1 hit. SM00355. ZnF_C2H2. 1 hit. [Graphical view] | ||||||||||||||||||||||||
| SUPFAM | SSF57903. FYVE_PHD_ZnF. 1 hit. | ||||||||||||||||||||||||
| PROSITE | PS50330. UIM. False negative. PS50178. ZF_FYVE. 1 hit. PS00028. ZINC_FINGER_C2H2_1. 1 hit. PS50157. ZINC_FINGER_C2H2_2. 1 hit. [Graphical view] | ||||||||||||||||||||||||
| ProtoNet | Search... | ||||||||||||||||||||||||
Other | |||||||||||||||||||||||||
| NextBio | 66042. | ||||||||||||||||||||||||
| SOURCE | Search... | ||||||||||||||||||||||||
Entry information
| Entry name | RBNS5_HUMAN | ||||||||
| Accession | Primary (citable) accession number: Q9H1K0 Secondary accession number(s): Q3KP30, Q59EY8, Q8NAQ1 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Chordata Protein Annotation Program | ||||||||
| Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. | ||||||||
Relevant documents
| Human chromosome 3 Human chromosome 3: entries, gene names and cross-references to MIM |
| Human entries with polymorphisms or disease mutations List of human entries with polymorphisms or disease mutations |
| Human polymorphisms and disease mutations Index of human polymorphisms and disease mutations |
| MIM cross-references Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot |
| PDB cross-references Index of Protein Data Bank (PDB) cross-references |
| SIMILARITY comments Index of protein domains and families |

Clusters with