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Protein

Rabenosyn-5

Gene

RBSN

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Rab4/Rab5 effector protein acting in early endocytic membrane fusion and membrane trafficking of recycling endosomes. Required for endosome fusion either homotypically or with clathrin coated vesicles. Plays a role in the lysosomal trafficking of CTSD/cathepsin D from the Golgi to lysosomes. Also promotes the recycling of transferrin directly from early endosomes to the plasma membrane. Binds phospholipid vesicles containing phosphatidylinositol 3-phosphate (PtdInsP3) (PubMed:11062261, PubMed:11788822, PubMed:15020713). Plays a role in the recycling of transferrin receptor to the plasma membrane (PubMed:22308388).4 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri14 – 37C2H2-typePROSITE-ProRule annotationAdd BLAST24
Zinc fingeri157 – 260FYVE-typePROSITE-ProRule annotationAdd BLAST104

GO - Molecular functioni

  • Rab GTPase binding Source: GO_Central
  • zinc ion binding Source: UniProtKB

GO - Biological processi

  • blood coagulation Source: Reactome
  • early endosome to Golgi transport Source: UniProtKB
  • endocytosis Source: GO_Central
  • endosomal transport Source: UniProtKB
  • endosomal vesicle fusion Source: GO_Central
  • Golgi to endosome transport Source: GO_Central
  • Golgi to lysosome transport Source: UniProtKB
  • Golgi to vacuole transport Source: GO_Central
  • protein transport Source: UniProtKB-KW
  • regulation of Golgi organization Source: UniProtKB
  • vacuole inheritance Source: GO_Central
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciZFISH:ENSG00000131381-MONOMER.
ReactomeiR-HSA-168138. Toll Like Receptor 9 (TLR9) Cascade.
R-HSA-983231. Factors involved in megakaryocyte development and platelet production.
SIGNORiQ9H1K0.

Names & Taxonomyi

Protein namesi
Recommended name:
Rabenosyn-51 Publication
Alternative name(s):
110 kDa protein
FYVE finger-containing Rab5 effector protein rabenosyn-5
RAB effector RBSNImported
Zinc finger FYVE domain-containing protein 20
Gene namesi
Name:RBSNImported
Synonyms:ZFYVE20Imported
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 3

Organism-specific databases

HGNCiHGNC:20759. RBSN.

Subcellular locationi

GO - Cellular componenti

  • cytoplasmic side of endosome membrane Source: GO_Central
  • cytosol Source: GOC
  • early endosome membrane Source: UniProtKB-SubCell
  • endosome Source: UniProtKB
  • endosome membrane Source: Reactome
  • extracellular exosome Source: UniProtKB
  • intracellular membrane-bounded organelle Source: HPA
  • plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Endosome, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi626 – 628NPF → APA: Reduces the interaction with EHD1. Abolishes the interaction with EHD1; when associated with 662-APA-664. 1 Publication3
Mutagenesisi662 – 664NPF → APA: Reduces the interaction with EHD1. Abolishes the interaction with EHD1; when associated with 626-APA-628. 1 Publication3

Organism-specific databases

DisGeNETi64145.
OpenTargetsiENSG00000131381.
PharmGKBiPA134959491.

Polymorphism and mutation databases

BioMutaiZFYVE20.
DMDMi108935884.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00000987112 – 784Rabenosyn-5Add BLAST783

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1
Modified residuei3PhosphoserineCombined sources1
Modified residuei215PhosphoserineBy similarity1
Modified residuei219PhosphoserineCombined sources1
Modified residuei226PhosphoserineCombined sources1
Modified residuei230PhosphoserineCombined sources1
Modified residuei684PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Lipoprotein, Phosphoprotein

Proteomic databases

EPDiQ9H1K0.
MaxQBiQ9H1K0.
PaxDbiQ9H1K0.
PeptideAtlasiQ9H1K0.
PRIDEiQ9H1K0.

PTM databases

iPTMnetiQ9H1K0.
PhosphoSitePlusiQ9H1K0.

Expressioni

Gene expression databases

BgeeiENSG00000131381.
CleanExiHS_ZFYVE20.
ExpressionAtlasiQ9H1K0. baseline and differential.
GenevisibleiQ9H1K0. HS.

Organism-specific databases

HPAiHPA044878.

Interactioni

Subunit structurei

Interacts with EHD1, RAB4A, RAB5A, RAB14, RAB22A, RAB24 and VPS45. Binds simultaneously to RAB4A and RAB5A in vitro. Interacts with RAB4A and RAB5A that has been activated by GTP binding.4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
EHD1Q9H4M93EBI-1105310,EBI-490691
VPS45Q9NRW73EBI-1105310,EBI-1782543

GO - Molecular functioni

Protein-protein interaction databases

BioGridi122084. 23 interactors.
IntActiQ9H1K0. 11 interactors.
MINTiMINT-2817361.
STRINGi9606.ENSP00000253699.

Structurei

Secondary structure

1784
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi458 – 475Combined sources18
Helixi479 – 500Combined sources22
Helixi736 – 755Combined sources20
Helixi759 – 779Combined sources21

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1YZMX-ray1.50A458-503[»]
1Z0JX-ray1.32B728-784[»]
1Z0KX-ray1.92B/D440-503[»]
ProteinModelPortaliQ9H1K0.
SMRiQ9H1K0.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9H1K0.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini496 – 515UIMCuratedAdd BLAST20

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni100 – 263Necessary for the correct targeting to endosomesAdd BLAST164
Regioni264 – 784Necessary for interaction with EHD11 PublicationAdd BLAST521
Regioni264 – 500Necessary for interaction with RAB4AAdd BLAST237
Regioni627 – 784Necessary for interaction with RAB5AAdd BLAST158

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili378 – 414Sequence analysisAdd BLAST37
Coiled coili472 – 531Sequence analysisAdd BLAST60

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi204 – 239Ser-richAdd BLAST36
Compositional biasi665 – 672Poly-Glu8

Sequence similaritiesi

Contains 1 C2H2-type zinc finger.PROSITE-ProRule annotation
Contains 1 FYVE-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri14 – 37C2H2-typePROSITE-ProRule annotationAdd BLAST24
Zinc fingeri157 – 260FYVE-typePROSITE-ProRule annotationAdd BLAST104

Keywords - Domaini

Coiled coil, Zinc-finger

Phylogenomic databases

eggNOGiKOG1842. Eukaryota.
ENOG410XP0U. LUCA.
GeneTreeiENSGT00390000007159.
HOGENOMiHOG000154082.
HOVERGENiHBG067237.
InParanoidiQ9H1K0.
KOiK12481.
OMAiKLRLCME.
OrthoDBiEOG091G06L4.
PhylomeDBiQ9H1K0.
TreeFamiTF106125.

Family and domain databases

Gene3Di3.30.40.10. 2 hits.
InterProiIPR021565. Rbsn_Rab_binding_dom.
IPR007087. Znf_C2H2.
IPR000306. Znf_FYVE.
IPR017455. Znf_FYVE-rel.
IPR011011. Znf_FYVE_PHD.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PfamiPF01363. FYVE. 1 hit.
PF11464. Rbsn. 2 hits.
[Graphical view]
SMARTiSM00064. FYVE. 1 hit.
[Graphical view]
SUPFAMiSSF57903. SSF57903. 2 hits.
PROSITEiPS50178. ZF_FYVE. 1 hit.
PS00028. ZINC_FINGER_C2H2_1. 1 hit.
PS50157. ZINC_FINGER_C2H2_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9H1K0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MASLDDPGEV REGFLCPLCL KDLQSFYQLH SHYEEEHSGE DRDVKGQIKS
60 70 80 90 100
LVQKAKKAKD RLLKREGDDR AESGTQGYES FSYGGVDPYM WEPQELGAVR
110 120 130 140 150
SHLSDFKKHR AARIDHYVVE VNKLIIRLEK LTAFDRTNTE SAKIRAIEKS
160 170 180 190 200
VVPWVNDQDV PFCPDCGNKF SIRNRRHHCR LCGSIMCKKC MELISLPLAN
210 220 230 240 250
KLTSASKESL STHTSPSQSP NSVHGSRRGS ISSMSSVSSV LDEKDDDRIR
260 270 280 290 300
CCTHCKDTLL KREQQIDEKE HTPDIVKLYE KLRLCMEKVD QKAPEYIRMA
310 320 330 340 350
ASLNAGETTY SLEHASDLRV EVQKVYELID ALSKKILTLG LNQDPPPHPS
360 370 380 390 400
NLRLQRMIRY SATLFVQEKL LGLMSLPTKE QFEELKKKRK EEMERKRAVE
410 420 430 440 450
RQAALESQRR LEERQSGLAS RAANGEVASL RRGPAPLRKA EGWLPLSGGQ
460 470 480 490 500
GQSEDSDPLL QQIHNITSFI RQAKAAGRMD EVRTLQENLR QLQDEYDQQQ
510 520 530 540 550
TEKAIELSRR QAEEEDLQRE QLQMLREREL EREREQFRVA SLHTRTRSLD
560 570 580 590 600
FREIGPFQLE PSREPRTHLA YALDLGSSPV PSSTAPKTPS LSSTQPTRVW
610 620 630 640 650
SGPPAVGQER LPQSSMPQQH EGPSLNPFDE EDLSSPMEEA TTGPPAAGVS
660 670 680 690 700
LDPSARILKE YNPFEEEDEE EEAVAGNPFI QPDSPAPNPF SEEDEHPQQR
710 720 730 740 750
LSSPLVPGNP FEEPTCINPF EMDSDSGPEA EEPIEEELLL QQIDNIKAYI
760 770 780
FDAKQCGRLD EVEVLTENLR ELKHTLAKQK GGTD
Length:784
Mass (Da):88,870
Last modified:June 13, 2006 - v2
Checksum:iD49E0E5A95B18616
GO
Isoform 2 (identifier: Q9H1K0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     200-208: NKLTSASKE → KITTLHGES
     209-784: Missing.

Note: No experimental confirmation available.
Show »
Length:208
Mass (Da):23,794
Checksum:iB416981A82661172
GO

Sequence cautioni

The sequence BAC03860 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAD92910 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti106F → L in AAG33246 (PubMed:11062261).Curated1
Sequence conflicti720F → L in AAG33246 (PubMed:11062261).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_072416425G → R Found in a patient with intractable epileptic encephalopathy, developmental delay and additional multi-organ symptoms; unknown pathological significance. 1 PublicationCorresponds to variant rs144008665dbSNPEnsembl.1
Natural variantiVAR_052982591L → P.Corresponds to variant rs9868848dbSNPEnsembl.1
Natural variantiVAR_052983641T → A.Corresponds to variant rs9851219dbSNPEnsembl.1
Natural variantiVAR_052984722M → I.Corresponds to variant rs9830744dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_056003200 – 208NKLTSASKE → KITTLHGES in isoform 2. 1 Publication9
Alternative sequenceiVSP_056004209 – 784Missing in isoform 2. 1 PublicationAdd BLAST576

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY009133 mRNA. Translation: AAG33246.1.
AK092312 mRNA. Translation: BAC03860.1. Different initiation.
AK301735 mRNA. Translation: BAG63200.1.
AC090954 Genomic DNA. No translation available.
BC106940 mRNA. Translation: AAI06941.1.
AB209673 mRNA. Translation: BAD92910.1. Different initiation.
CCDSiCCDS2623.1. [Q9H1K0-1]
RefSeqiNP_001289307.1. NM_001302378.1. [Q9H1K0-1]
NP_071735.2. NM_022340.3. [Q9H1K0-1]
XP_005265441.1. XM_005265384.4. [Q9H1K0-1]
XP_005265442.1. XM_005265385.4. [Q9H1K0-1]
XP_016862512.1. XM_017007023.1. [Q9H1K0-1]
UniGeneiHs.475565.
Hs.706478.

Genome annotation databases

EnsembliENST00000253699; ENSP00000253699; ENSG00000131381. [Q9H1K0-1]
ENST00000476527; ENSP00000422551; ENSG00000131381. [Q9H1K0-1]
GeneIDi64145.
KEGGihsa:64145.
UCSCiuc003bzm.1. human. [Q9H1K0-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY009133 mRNA. Translation: AAG33246.1.
AK092312 mRNA. Translation: BAC03860.1. Different initiation.
AK301735 mRNA. Translation: BAG63200.1.
AC090954 Genomic DNA. No translation available.
BC106940 mRNA. Translation: AAI06941.1.
AB209673 mRNA. Translation: BAD92910.1. Different initiation.
CCDSiCCDS2623.1. [Q9H1K0-1]
RefSeqiNP_001289307.1. NM_001302378.1. [Q9H1K0-1]
NP_071735.2. NM_022340.3. [Q9H1K0-1]
XP_005265441.1. XM_005265384.4. [Q9H1K0-1]
XP_005265442.1. XM_005265385.4. [Q9H1K0-1]
XP_016862512.1. XM_017007023.1. [Q9H1K0-1]
UniGeneiHs.475565.
Hs.706478.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1YZMX-ray1.50A458-503[»]
1Z0JX-ray1.32B728-784[»]
1Z0KX-ray1.92B/D440-503[»]
ProteinModelPortaliQ9H1K0.
SMRiQ9H1K0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi122084. 23 interactors.
IntActiQ9H1K0. 11 interactors.
MINTiMINT-2817361.
STRINGi9606.ENSP00000253699.

PTM databases

iPTMnetiQ9H1K0.
PhosphoSitePlusiQ9H1K0.

Polymorphism and mutation databases

BioMutaiZFYVE20.
DMDMi108935884.

Proteomic databases

EPDiQ9H1K0.
MaxQBiQ9H1K0.
PaxDbiQ9H1K0.
PeptideAtlasiQ9H1K0.
PRIDEiQ9H1K0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000253699; ENSP00000253699; ENSG00000131381. [Q9H1K0-1]
ENST00000476527; ENSP00000422551; ENSG00000131381. [Q9H1K0-1]
GeneIDi64145.
KEGGihsa:64145.
UCSCiuc003bzm.1. human. [Q9H1K0-1]

Organism-specific databases

CTDi64145.
DisGeNETi64145.
GeneCardsiRBSN.
HGNCiHGNC:20759. RBSN.
HPAiHPA044878.
MIMi609511. gene.
neXtProtiNX_Q9H1K0.
OpenTargetsiENSG00000131381.
PharmGKBiPA134959491.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1842. Eukaryota.
ENOG410XP0U. LUCA.
GeneTreeiENSGT00390000007159.
HOGENOMiHOG000154082.
HOVERGENiHBG067237.
InParanoidiQ9H1K0.
KOiK12481.
OMAiKLRLCME.
OrthoDBiEOG091G06L4.
PhylomeDBiQ9H1K0.
TreeFamiTF106125.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000131381-MONOMER.
ReactomeiR-HSA-168138. Toll Like Receptor 9 (TLR9) Cascade.
R-HSA-983231. Factors involved in megakaryocyte development and platelet production.
SIGNORiQ9H1K0.

Miscellaneous databases

ChiTaRSiZFYVE20. human.
EvolutionaryTraceiQ9H1K0.
GeneWikiiZFYVE20.
GenomeRNAii64145.
PROiQ9H1K0.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000131381.
CleanExiHS_ZFYVE20.
ExpressionAtlasiQ9H1K0. baseline and differential.
GenevisibleiQ9H1K0. HS.

Family and domain databases

Gene3Di3.30.40.10. 2 hits.
InterProiIPR021565. Rbsn_Rab_binding_dom.
IPR007087. Znf_C2H2.
IPR000306. Znf_FYVE.
IPR017455. Znf_FYVE-rel.
IPR011011. Znf_FYVE_PHD.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PfamiPF01363. FYVE. 1 hit.
PF11464. Rbsn. 2 hits.
[Graphical view]
SMARTiSM00064. FYVE. 1 hit.
[Graphical view]
SUPFAMiSSF57903. SSF57903. 2 hits.
PROSITEiPS50178. ZF_FYVE. 1 hit.
PS00028. ZINC_FINGER_C2H2_1. 1 hit.
PS50157. ZINC_FINGER_C2H2_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRBNS5_HUMAN
AccessioniPrimary (citable) accession number: Q9H1K0
Secondary accession number(s): B4DWY8
, C9J4P5, Q3KP30, Q59EY8, Q8NAQ1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2005
Last sequence update: June 13, 2006
Last modified: November 30, 2016
This is version 145 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 3
    Human chromosome 3: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.