Q9H1J7 (WNT5B_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein Wnt-5b

Gene names

Name:

WNT5B

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	359 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters By similarity.
Subunit structure	Interacts with PORCN By similarity.
Subcellular location	Secreted › extracellular space › extracellular matrix.
Post-translational modification	Palmitoylation at Ser-223 is required for efficient binding to frizzled receptors. It is also required for subsequent palmitoylation at Cys-83. Palmitoylation is necessary for proper trafficking to cell surface By similarity.
Sequence similarities	Belongs to the Wnt family.

Ontologies

Keywords
Biological process	Wnt signaling pathway
Cellular component	Extracellular matrix Secreted
Domain	Signal
Molecular function	Developmental protein
PTM	Glycoprotein Lipoprotein Palmitate
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	Wnt receptor signaling pathway, calcium modulating pathway Inferred from Biological aspect of Ancestor. Source: RefGenome angiogenesis Inferred from Biological aspect of Ancestor. Source: RefGenome anterior/posterior pattern specification Inferred from Biological aspect of Ancestor. Source: RefGenome cell migration involved in gastrulation Inferred from Biological aspect of Ancestor. Source: RefGenome cellular response to retinoic acid Inferred from sequence or structural similarity. Source: UniProtKB chondrocyte differentiation Inferred from expression pattern PubMed 15135146. Source: UniProtKB convergent extension involved in axis elongation Inferred from Biological aspect of Ancestor. Source: RefGenome convergent extension involved in gastrulation Inferred from Biological aspect of Ancestor. Source: RefGenome dorsal/ventral axis specification Inferred from Biological aspect of Ancestor. Source: RefGenome endocrine pancreas development Inferred from Biological aspect of Ancestor. Source: RefGenome fat cell differentiation Non-traceable author statement PubMed 19577541. Source: UniProtKB lens fiber cell development Inferred from sequence or structural similarity PubMed 16258938. Source: BHF-UCL negative regulation of canonical Wnt receptor signaling pathway Inferred from Biological aspect of Ancestor. Source: RefGenome neuron differentiation Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of cell migration Inferred from direct assay PubMed 19486338. Source: UniProtKB positive regulation of fat cell differentiation Inferred from Biological aspect of Ancestor. Source: RefGenome respiratory system development Inferred from Biological aspect of Ancestor. Source: RefGenome
Cellular_component	cell surface Inferred from electronic annotation. Source: Compara extracellular space Non-traceable author statement PubMed 15135146. Source: UniProtKB plasma membrane Inferred from Biological aspect of Ancestor. Source: RefGenome proteinaceous extracellular matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	frizzled-2 binding Inferred from Biological aspect of Ancestor. Source: RefGenome
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 17	17	Potential
	Chain	18 – 359	342	Protein Wnt-5b		PRO_0000041434
Amino acid modifications
	Lipidation	83	1	S-palmitoyl cysteine By similarity
	Lipidation	223	1	O-palmitoyl serine; by PORCN By similarity
	Glycosylation	93	1	N-linked (GlcNAc...) Potential
	Glycosylation	99	1	N-linked (GlcNAc...) Potential
	Glycosylation	291	1	N-linked (GlcNAc...) Potential
	Glycosylation	305	1	N-linked (GlcNAc...) Potential
Experimental info
	Sequence conflict	73	1	G → R in AAG38659. Ref.1
	Sequence conflict	88	1	R → P in AAG38659. Ref.1
	Sequence conflict	93	1	N → K in AAG38659. Ref.1
	Sequence conflict	134	1	R → S in AAG38659. Ref.1
	Sequence conflict	224	1	G → R in AAG38659. Ref.1
	Sequence conflict	227	1	S → R in AAG38659. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q9H1J7 [UniParc]. Last modified May 10, 2002. Version 2. Checksum: 6E35EE2B0AF1FD29	FASTA	359	40,323
	10 20 30 40 50 60 MPSLLLLFTA ALLSSWAQLL TDANSWWSLA LNPVQRPEMF IIGAQPVCSQ LPGLSPGQRK 70 80 90 100 110 120 LCQLYQEHMA YIGEGAKTGI KECQHQFRQR RWNCSTADNA SVFGRVMQIG SRETAFTHAV 130 140 150 160 170 180 SAAGVVNAIS RACREGELST CGCSRTARPK DLPRDWLWGG CGDNVEYGYR FAKEFVDARE 190 200 210 220 230 240 REKNFAKGSE EQGRVLMNLQ NNEAGRRAVY KMADVACKCH GVSGSCSLKT CWLQLAEFRK 250 260 270 280 290 300 VGDRLKEKYD SAAAMRVTRK GRLELVNSRF TQPTPEDLVY VDPSPDYCLR NESTGSLGTQ 310 320 330 340 350 GRLCNKTSEG MDGCELMCCG RGYNQFKSVQ VERCHCKFHW CCFVRCKKCT EIVDQYICK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning and characterization of six novel human WNT genes." Testa T.T., Mossakowska D.E., Carter P.S., Hu E., Zhu Y., Kelsell D.P., Murdock P.R., Herrity N.C., Lewis C.J., Cross D.A., Culbert A.A., Reith A.D., Barnes M.R. Submitted (AUG-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Molecular cloning and characterization of human WNT5B on chromosome 12p13.3 region." Saitoh T., Katoh M. Int. J. Oncol. 19:347-351(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Muscle.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY009399 mRNA. Translation: AAG38659.1. AB060966 mRNA. Translation: BAB62039.1. AK290430 mRNA. Translation: BAF83119.1. CH471116 Genomic DNA. Translation: EAW88925.1. CH471116 Genomic DNA. Translation: EAW88927.1. BC001749 mRNA. Translation: AAH01749.1.
IPI	IPI00022223.
RefSeq	NP_110402.2. NM_030775.2. NP_116031.1. NM_032642.2.
UniGene	Hs.306051.
3D structure databases
ProteinModelPortal	Q9H1J7.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9H1J7. 1 interaction.
MINT	MINT-1485183.
STRING	9606.ENSP00000308887.
PTM databases
PhosphoSite	Q9H1J7.
Polymorphism databases
DMDM	20532427.
Proteomic databases
PaxDb	Q9H1J7.
PRIDE	Q9H1J7.
Protocols and materials databases
DNASU	81029.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000310594; ENSP00000308887; ENSG00000111186. ENST00000397196; ENSP00000380379; ENSG00000111186. ENST00000537031; ENSP00000439312; ENSG00000111186.
GeneID	81029.
KEGG	hsa:81029.
UCSC	uc001qjj.3. human.
Organism-specific databases
CTD	81029.
GeneCards	GC12P001639.
HGNC	HGNC:16265. WNT5B.
MIM	606361. gene.
neXtProt	NX_Q9H1J7.
PharmGKB	PA38104.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG284879.
HOGENOM	HOG000039529.
HOVERGEN	HBG001595.
InParanoid	Q9H1J7.
KO	K00444.
OMA	WWSLALN.
OrthoDB	EOG4H19VP.
PhylomeDB	Q9H1J7.
Gene expression databases
ArrayExpress	Q9H1J7.
Bgee	Q9H1J7.
CleanEx	HS_WNT5B.
Genevestigator	Q9H1J7.
GermOnline	ENSG00000111186. Homo sapiens.
Family and domain databases
InterPro	IPR005817. Wnt. IPR026537. Wnt5b. IPR018161. Wnt_CS. [Graphical view]
PANTHER	PTHR12027. PTHR12027. 1 hit. PTHR12027:SF23. PTHR12027:SF23. 1 hit.
Pfam	PF00110. wnt. 1 hit. [Graphical view]
PRINTS	PR01349. WNTPROTEIN.
SMART	SM00097. WNT1. 1 hit. [Graphical view]
PROSITE	PS00246. WNT1. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	WNT5B. human.
GenomeRNAi	81029.
NextBio	71352.
SOURCE	Search...

Entry information

Entry name

WNT5B_HUMAN

Accession

Primary (citable) accession number: Q9H1J7
Secondary accession number(s): A8K315 , D3DUP9, Q96S49, Q9BV04

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 20, 2001
Last sequence update:	May 10, 2002
Last modified:	April 3, 2013
This is version 105 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.