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Q9H1J7 (WNT5B_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 114. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein Wnt-5b
Gene names
Name:WNT5B
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length359 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters By similarity.

Subunit structure

Interacts with PORCN By similarity.

Subcellular location

Secretedextracellular spaceextracellular matrix.

Post-translational modification

Palmitoylation at Ser-223 is required for efficient binding to frizzled receptors. It is also required for subsequent palmitoylation at Cys-83. Palmitoylation is necessary for proper trafficking to cell surface By similarity.

Sequence similarities

Belongs to the Wnt family.

Ontologies

Keywords
   Biological processWnt signaling pathway
   Cellular componentExtracellular matrix
Secreted
   DomainSignal
   Molecular functionDevelopmental protein
   PTMGlycoprotein
Lipoprotein
Palmitate
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processWnt signaling pathway

Inferred from direct assay PubMed 15731909. Source: UniProtKB

cell fate commitment

Inferred from Biological aspect of Ancestor. Source: RefGenome

cellular response to retinoic acid

Inferred from sequence or structural similarity. Source: UniProtKB

chondrocyte differentiation

Inferred from expression pattern PubMed 15135146. Source: UniProtKB

fat cell differentiation

Non-traceable author statement PubMed 19577541. Source: UniProtKB

lens fiber cell development

Inferred from sequence or structural similarity PubMed 16258938. Source: BHF-UCL

negative regulation of canonical Wnt signaling pathway

Inferred from electronic annotation. Source: Ensembl

neuron differentiation

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of cell migration

Inferred from direct assay PubMed 19486338. Source: UniProtKB

positive regulation of fat cell differentiation

Inferred from electronic annotation. Source: Ensembl

wound healing

Inferred from electronic annotation. Source: Ensembl

   Cellular_componentGolgi lumen

Traceable author statement. Source: Reactome

cell surface

Inferred from electronic annotation. Source: Ensembl

endoplasmic reticulum lumen

Traceable author statement. Source: Reactome

extracellular region

Traceable author statement. Source: Reactome

extracellular space

Non-traceable author statement PubMed 15135146. Source: UniProtKB

extracellular vesicular exosome

Inferred from direct assay PubMed 19056867. Source: UniProt

plasma membrane

Traceable author statement. Source: Reactome

proteinaceous extracellular matrix

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionfrizzled binding

Inferred from Biological aspect of Ancestor. Source: RefGenome

receptor binding

Inferred from physical interaction PubMed 19486338. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1717 Potential
Chain18 – 359342Protein Wnt-5b
PRO_0000041434

Amino acid modifications

Lipidation831S-palmitoyl cysteine By similarity
Lipidation2231O-palmitoyl serine; by PORCN By similarity
Glycosylation931N-linked (GlcNAc...) Potential
Glycosylation991N-linked (GlcNAc...) Potential
Glycosylation2911N-linked (GlcNAc...) Potential
Glycosylation3051N-linked (GlcNAc...) Potential

Experimental info

Sequence conflict731G → R in AAG38659. Ref.1
Sequence conflict881R → P in AAG38659. Ref.1
Sequence conflict931N → K in AAG38659. Ref.1
Sequence conflict1341R → S in AAG38659. Ref.1
Sequence conflict2241G → R in AAG38659. Ref.1
Sequence conflict2271S → R in AAG38659. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q9H1J7 [UniParc].

Last modified May 10, 2002. Version 2.
Checksum: 6E35EE2B0AF1FD29

FASTA35940,323
        10         20         30         40         50         60 
MPSLLLLFTA ALLSSWAQLL TDANSWWSLA LNPVQRPEMF IIGAQPVCSQ LPGLSPGQRK 

        70         80         90        100        110        120 
LCQLYQEHMA YIGEGAKTGI KECQHQFRQR RWNCSTADNA SVFGRVMQIG SRETAFTHAV 

       130        140        150        160        170        180 
SAAGVVNAIS RACREGELST CGCSRTARPK DLPRDWLWGG CGDNVEYGYR FAKEFVDARE 

       190        200        210        220        230        240 
REKNFAKGSE EQGRVLMNLQ NNEAGRRAVY KMADVACKCH GVSGSCSLKT CWLQLAEFRK 

       250        260        270        280        290        300 
VGDRLKEKYD SAAAMRVTRK GRLELVNSRF TQPTPEDLVY VDPSPDYCLR NESTGSLGTQ 

       310        320        330        340        350 
GRLCNKTSEG MDGCELMCCG RGYNQFKSVQ VERCHCKFHW CCFVRCKKCT EIVDQYICK 

« Hide

References

« Hide 'large scale' references
[1]"Molecular cloning and characterization of six novel human WNT genes."
Testa T.T., Mossakowska D.E., Carter P.S., Hu E., Zhu Y., Kelsell D.P., Murdock P.R., Herrity N.C., Lewis C.J., Cross D.A., Culbert A.A., Reith A.D., Barnes M.R.
Submitted (AUG-2000) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Molecular cloning and characterization of human WNT5B on chromosome 12p13.3 region."
Saitoh T., Katoh M.
Int. J. Oncol. 19:347-351(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Muscle.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY009399 mRNA. Translation: AAG38659.1.
AB060966 mRNA. Translation: BAB62039.1.
AK290430 mRNA. Translation: BAF83119.1.
CH471116 Genomic DNA. Translation: EAW88925.1.
CH471116 Genomic DNA. Translation: EAW88927.1.
BC001749 mRNA. Translation: AAH01749.1.
RefSeqNP_110402.2. NM_030775.2.
NP_116031.1. NM_032642.2.
UniGeneHs.306051.

3D structure databases

ProteinModelPortalQ9H1J7.
SMRQ9H1J7. Positions 73-288.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid123348. 2 interactions.
IntActQ9H1J7. 1 interaction.
MINTMINT-1485183.
STRING9606.ENSP00000308887.

PTM databases

PhosphoSiteQ9H1J7.

Polymorphism databases

DMDM20532427.

Proteomic databases

PaxDbQ9H1J7.
PRIDEQ9H1J7.

Protocols and materials databases

DNASU81029.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000310594; ENSP00000308887; ENSG00000111186.
ENST00000397196; ENSP00000380379; ENSG00000111186.
ENST00000537031; ENSP00000439312; ENSG00000111186.
GeneID81029.
KEGGhsa:81029.
UCSCuc001qjj.3. human.

Organism-specific databases

CTD81029.
GeneCardsGC12P001639.
HGNCHGNC:16265. WNT5B.
MIM606361. gene.
neXtProtNX_Q9H1J7.
PharmGKBPA38104.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG284879.
HOGENOMHOG000039529.
HOVERGENHBG001595.
InParanoidQ9H1J7.
KOK00444.
OMALTDANSW.
PhylomeDBQ9H1J7.
TreeFamTF105310.

Enzyme and pathway databases

ReactomeREACT_111102. Signal Transduction.

Gene expression databases

ArrayExpressQ9H1J7.
BgeeQ9H1J7.
CleanExHS_WNT5B.
GenevestigatorQ9H1J7.

Family and domain databases

InterProIPR005817. Wnt.
IPR026537. Wnt5b.
IPR018161. Wnt_CS.
[Graphical view]
PANTHERPTHR12027. PTHR12027. 1 hit.
PTHR12027:SF23. PTHR12027:SF23. 1 hit.
PfamPF00110. wnt. 1 hit.
[Graphical view]
PRINTSPR01349. WNTPROTEIN.
SMARTSM00097. WNT1. 1 hit.
[Graphical view]
PROSITEPS00246. WNT1. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSWNT5B. human.
GeneWikiWNT5B.
GenomeRNAi81029.
NextBio71352.
PROQ9H1J7.
SOURCESearch...

Entry information

Entry nameWNT5B_HUMAN
AccessionPrimary (citable) accession number: Q9H1J7
Secondary accession number(s): A8K315 expand/collapse secondary AC list , D3DUP9, Q96S49, Q9BV04
Entry history
Integrated into UniProtKB/Swiss-Prot: June 20, 2001
Last sequence update: May 10, 2002
Last modified: April 16, 2014
This is version 114 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 12

Human chromosome 12: entries, gene names and cross-references to MIM