Q9H1J5 (WNT8A_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein Wnt-8a

Alternative name(s):
Protein Wnt-8d

Gene names

Name:	WNT8A
Synonyms:	WNT8D

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	351 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Ligand for members of the frizzled family of seven transmembrane receptors. May play an important role in the development and differentiation of certain forebrain structures, notably the hippocampus.
Subcellular location	Secreted › extracellular space › extracellular matrix.
Sequence similarities	Belongs to the Wnt family.

Ontologies

Keywords
Biological process	Wnt signaling pathway
Cellular component	Extracellular matrix Secreted
Coding sequence diversity	Alternative splicing
Domain	Signal
Molecular function	Developmental protein
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	brain segmentation Inferred from Biological aspect of Ancestor. Source: RefGenome canonical Wnt receptor signaling pathway involved in cardiac muscle cell fate commitment Inferred from expression pattern PubMed 20559569. Source: BHF-UCL canonical Wnt receptor signaling pathway involved in neural crest cell differentiation Inferred from sequence or structural similarity. Source: BHF-UCL cell migration involved in gastrulation Inferred from Biological aspect of Ancestor. Source: RefGenome dorsal/ventral pattern formation Inferred from Biological aspect of Ancestor. Source: RefGenome ectoderm development Inferred from Biological aspect of Ancestor. Source: RefGenome endoderm development Inferred from Biological aspect of Ancestor. Source: RefGenome establishment of organ orientation Inferred from electronic annotation. Source: Compara eye development Inferred from Biological aspect of Ancestor. Source: RefGenome hindbrain development Inferred from Biological aspect of Ancestor. Source: RefGenome mesodermal cell fate commitment Inferred from Biological aspect of Ancestor. Source: RefGenome negative regulation of Wnt receptor signaling pathway Inferred from Biological aspect of Ancestor. Source: RefGenome neural crest cell fate commitment Inferred from sequence or structural similarity. Source: BHF-UCL neural plate pattern specification Inferred from Biological aspect of Ancestor. Source: RefGenome notochord development Inferred from Biological aspect of Ancestor. Source: RefGenome palate development Inferred from mutant phenotype PubMed 18413325. Source: BHF-UCL polarity specification of anterior/posterior axis Inferred from Biological aspect of Ancestor. Source: RefGenome polarity specification of proximal/distal axis Inferred from Biological aspect of Ancestor. Source: RefGenome positive regulation of fibroblast growth factor receptor signaling pathway Inferred from Biological aspect of Ancestor. Source: RefGenome regulation of protein localization Inferred from electronic annotation. Source: Compara regulation of transcription involved in anterior/posterior axis specification Inferred from sequence or structural similarity. Source: BHF-UCL response to retinoic acid Non-traceable author statement PubMed 11956596. Source: BHF-UCL somitogenesis Inferred from Biological aspect of Ancestor. Source: RefGenome spinal cord anterior/posterior patterning Inferred from Biological aspect of Ancestor. Source: RefGenome
Cellular_component	extracellular space Inferred from Biological aspect of Ancestor. Source: RefGenome plasma membrane Inferred from Biological aspect of Ancestor. Source: RefGenome proteinaceous extracellular matrix Inferred from sequence or structural similarity. Source: BHF-UCL
Molecular_function	frizzled binding Non-traceable author statement Ref.1. Source: BHF-UCL receptor agonist activity Non-traceable author statement Ref.1. Source: BHF-UCL
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9H1J5-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9H1J5-2)

The sequence of this isoform differs from the canonical sequence as follows:
     348-351: KGSA → RVWFGVYI

Note: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 24	24	Potential
	Chain	25 – 351	327	Protein Wnt-8a		PRO_0000041448
Amino acid modifications
	Glycosylation	103	1	N-linked (GlcNAc...) Potential
	Glycosylation	262	1	N-linked (GlcNAc...) Potential
	Glycosylation	281	1	N-linked (GlcNAc...) Potential
Natural variations
	Alternative sequence	348 – 351	4	KGSA → RVWFGVYI in isoform 2.		VSP_038706

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified February 9, 2010. Version 2. Checksum: B3432171301C17E5	FASTA	351	38,849
	10 20 30 40 50 60 MGNLFMLWAA LGICCAAFSA SAWSVNNFLI TGPKAYLTYT TSVALGAQSG IEECKFQFAW 70 80 90 100 110 120 ERWNCPENAL QLSTHNRLRS ATRETSFIHA ISSAGVMYII TKNCSMGDFE NCGCDGSNNG 130 140 150 160 170 180 KTGGHGWIWG GCSDNVEFGE RISKLFVDSL EKGKDARALM NLHNNRAGRL AVRATMKRTC 190 200 210 220 230 240 KCHGISGSCS IQTCWLQLAE FREMGDYLKA KYDQALKIEM DKRQLRAGNS AEGHWVPAEA 250 260 270 280 290 300 FLPSAEAELI FLEESPDYCT CNSSLGIYGT EGRECLQNSH NTSRWERRSC GRLCTECGLQ 310 320 330 340 350 VEERKTEVIS SCNCKFQWCC TVKCDQCRHV VSKYYCARSP GSAQSLGKGS A
	Isoform 2 [UniParc]. Checksum: 605B1D5DD755FBD6	FASTA	355	39,527
	10 20 30 40 50 60 MGNLFMLWAA LGICCAAFSA SAWSVNNFLI TGPKAYLTYT TSVALGAQSG IEECKFQFAW 70 80 90 100 110 120 ERWNCPENAL QLSTHNRLRS ATRETSFIHA ISSAGVMYII TKNCSMGDFE NCGCDGSNNG 130 140 150 160 170 180 KTGGHGWIWG GCSDNVEFGE RISKLFVDSL EKGKDARALM NLHNNRAGRL AVRATMKRTC 190 200 210 220 230 240 KCHGISGSCS IQTCWLQLAE FREMGDYLKA KYDQALKIEM DKRQLRAGNS AEGHWVPAEA 250 260 270 280 290 300 FLPSAEAELI FLEESPDYCT CNSSLGIYGT EGRECLQNSH NTSRWERRSC GRLCTECGLQ 310 320 330 340 350 VEERKTEVIS SCNCKFQWCC TVKCDQCRHV VSKYYCARSP GSAQSLGRVW FGVYI

References

[1]	"Molecular cloning and characterization of human WNT8A." Saitoh T., Katoh M. Int. J. Oncol. 19:123-127(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]	"Molecular cloning and characterization of six novel human WNT genes." Testa T.T., Mossakowska D.E., Carter P.S., Hu E., Zhu Y., Kelsell D.P., Murdock P.R., Herrity N.C., Lewis C.J., Cross D.A., Culbert A.A., Reith A.D., Barnes M.R. Submitted (AUG-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB057725 mRNA. Translation: BAB60960.1. AY009402 mRNA. Translation: AAG38662.1.
IPI	IPI00022222. IPI00926615.
RefSeq	NP_490645.1. NM_058244.2.
UniGene	Hs.591274.
3D structure databases
ProteinModelPortal	Q9H1J5.
SMR	Q9H1J5. Positions 31-337.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000381739.
PTM databases
PhosphoSite	Q9H1J5.
Polymorphism databases
DMDM	288558833.
2D gel databases
REPRODUCTION-2DPAGE	Q9H1J5.
Proteomic databases
PaxDb	Q9H1J5.
PRIDE	Q9H1J5.
Protocols and materials databases
DNASU	7478.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000361560; ENSP00000354726; ENSG00000061492. ENST00000398754; ENSP00000381739; ENSG00000061492.
GeneID	7478.
KEGG	hsa:7478.
UCSC	uc003lcd.1. human.
Organism-specific databases
CTD	7478.
GeneCards	GC05P137447.
HGNC	HGNC:12788. WNT8A.
HPA	HPA038539.
MIM	606360. gene.
neXtProt	NX_Q9H1J5.
PharmGKB	PA37389.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG277037.
HOGENOM	HOG000039529.
HOVERGEN	HBG001595.
InParanoid	Q9H1J5.
KO	K00714.
Enzyme and pathway databases
Reactome	REACT_111102. Signal Transduction.
Gene expression databases
ArrayExpress	Q9H1J5.
Bgee	Q9H1J5.
CleanEx	HS_WNT8A.
Genevestigator	Q9H1J5.
GermOnline	ENSG00000061492. Homo sapiens.
Family and domain databases
InterPro	IPR005817. Wnt. IPR013301. Wnt8. IPR018161. Wnt_CS. [Graphical view]
PANTHER	PTHR12027. PTHR12027. 1 hit. PTHR12027:SF6. PTHR12027:SF6. 1 hit.
Pfam	PF00110. wnt. 1 hit. [Graphical view]
PRINTS	PR01892. WNT8PROTEIN. PR01349. WNTPROTEIN.
SMART	SM00097. WNT1. 1 hit. [Graphical view]
PROSITE	PS00246. WNT1. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	7478.
NextBio	29292.
SOURCE	Search...

Entry information

Entry name

WNT8A_HUMAN

Accession

Primary (citable) accession number: Q9H1J5
Secondary accession number(s): Q96S51

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 20, 2001
Last sequence update:	February 9, 2010
Last modified:	April 3, 2013
This is version 100 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.