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Protein

Protein Wnt-8a

Gene

WNT8A

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Ligand for members of the frizzled family of seven transmembrane receptors. May play an important role in the development and differentiation of certain forebrain structures, notably the hippocampus.

GO - Molecular functioni

  • frizzled binding Source: BHF-UCL
  • receptor agonist activity Source: BHF-UCL

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Wnt signaling pathway

Enzyme and pathway databases

ReactomeiREACT_163710. WNT ligand biogenesis and trafficking.
REACT_18372. Class B/2 (Secretin family receptors).
REACT_264034. disassembly of the destruction complex and recruitment of AXIN to the membrane.
REACT_264596. TCF dependent signaling in response to WNT.
SignaLinkiQ9H1J5.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein Wnt-8a
Alternative name(s):
Protein Wnt-8d
Gene namesi
Name:WNT8A
Synonyms:WNT8D
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 5

Organism-specific databases

HGNCiHGNC:12788. WNT8A.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Extracellular matrix, Secreted

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA37389.

Polymorphism and mutation databases

BioMutaiWNT8A.
DMDMi288558833.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2424Sequence AnalysisAdd
BLAST
Chaini25 – 351327Protein Wnt-8aPRO_0000041448Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi54 ↔ 65By similarity
Glycosylationi103 – 1031N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi104 ↔ 112By similarity
Disulfide bondi114 ↔ 132By similarity
Disulfide bondi180 ↔ 194By similarity
Disulfide bondi182 ↔ 189By similarity
Lipidationi186 – 1861O-palmitoleyl serineBy similarity
Disulfide bondi259 ↔ 297By similarity
Glycosylationi262 – 2621N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi275 ↔ 290By similarity
Glycosylationi281 – 2811N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi294 ↔ 336By similarity
Disulfide bondi312 ↔ 327By similarity
Disulfide bondi314 ↔ 324By similarity
Disulfide bondi319 ↔ 320By similarity

Post-translational modificationi

Palmitoleylation is required for efficient binding to frizzled receptors (By similarity). Depalmitoleylation leads to Wnt signaling pathway inhibition (By similarity).By similarity
Proteolytic processing by TIKI1 and TIKI2 promotes oxidation and formation of large disulfide-bond oligomers, leading to inactivation of WNT8A.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Lipoprotein

Proteomic databases

PaxDbiQ9H1J5.
PRIDEiQ9H1J5.

2D gel databases

REPRODUCTION-2DPAGEQ9H1J5.

PTM databases

PhosphoSiteiQ9H1J5.

Expressioni

Gene expression databases

BgeeiQ9H1J5.
CleanExiHS_WNT8A.
ExpressionAtlasiQ9H1J5. baseline and differential.
GenevisibleiQ9H1J5. HS.

Organism-specific databases

HPAiHPA038539.

Interactioni

Protein-protein interaction databases

BioGridi113315. 1 interaction.
STRINGi9606.ENSP00000381739.

Structurei

3D structure databases

ProteinModelPortaliQ9H1J5.
SMRiQ9H1J5. Positions 31-337.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the Wnt family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG277037.
GeneTreeiENSGT00690000101857.
HOGENOMiHOG000039529.
HOVERGENiHBG001595.
InParanoidiQ9H1J5.
KOiK00714.
OrthoDBiEOG7C8GJ8.
PhylomeDBiQ9H1J5.
TreeFamiTF105310.

Family and domain databases

InterProiIPR005817. Wnt.
IPR013301. Wnt8.
IPR018161. Wnt_CS.
[Graphical view]
PANTHERiPTHR12027. PTHR12027. 1 hit.
PTHR12027:SF92. PTHR12027:SF92. 1 hit.
PfamiPF00110. wnt. 1 hit.
[Graphical view]
PRINTSiPR01892. WNT8PROTEIN.
PR01349. WNTPROTEIN.
SMARTiSM00097. WNT1. 1 hit.
[Graphical view]
PROSITEiPS00246. WNT1. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9H1J5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGNLFMLWAA LGICCAAFSA SAWSVNNFLI TGPKAYLTYT TSVALGAQSG
60 70 80 90 100
IEECKFQFAW ERWNCPENAL QLSTHNRLRS ATRETSFIHA ISSAGVMYII
110 120 130 140 150
TKNCSMGDFE NCGCDGSNNG KTGGHGWIWG GCSDNVEFGE RISKLFVDSL
160 170 180 190 200
EKGKDARALM NLHNNRAGRL AVRATMKRTC KCHGISGSCS IQTCWLQLAE
210 220 230 240 250
FREMGDYLKA KYDQALKIEM DKRQLRAGNS AEGHWVPAEA FLPSAEAELI
260 270 280 290 300
FLEESPDYCT CNSSLGIYGT EGRECLQNSH NTSRWERRSC GRLCTECGLQ
310 320 330 340 350
VEERKTEVIS SCNCKFQWCC TVKCDQCRHV VSKYYCARSP GSAQSLGKGS

A
Length:351
Mass (Da):38,849
Last modified:February 9, 2010 - v2
Checksum:iB3432171301C17E5
GO
Isoform 2 (identifier: Q9H1J5-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     348-351: KGSA → RVWFGVYI

Note: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.
Show »
Length:355
Mass (Da):39,527
Checksum:i605B1D5DD755FBD6
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei348 – 3514KGSA → RVWFGVYI in isoform 2. 1 PublicationVSP_038706

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB057725 mRNA. Translation: BAB60960.1.
AY009402 mRNA. Translation: AAG38662.1.
CCDSiCCDS43368.1. [Q9H1J5-1]
RefSeqiNP_001287867.1. NM_001300938.1.
NP_001287868.1. NM_001300939.1.
NP_490645.1. NM_058244.3. [Q9H1J5-1]
UniGeneiHs.591274.

Genome annotation databases

EnsembliENST00000361560; ENSP00000354726; ENSG00000061492. [Q9H1J5-2]
ENST00000398754; ENSP00000381739; ENSG00000061492. [Q9H1J5-1]
GeneIDi7478.
KEGGihsa:7478.
UCSCiuc003lcd.1. human. [Q9H1J5-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB057725 mRNA. Translation: BAB60960.1.
AY009402 mRNA. Translation: AAG38662.1.
CCDSiCCDS43368.1. [Q9H1J5-1]
RefSeqiNP_001287867.1. NM_001300938.1.
NP_001287868.1. NM_001300939.1.
NP_490645.1. NM_058244.3. [Q9H1J5-1]
UniGeneiHs.591274.

3D structure databases

ProteinModelPortaliQ9H1J5.
SMRiQ9H1J5. Positions 31-337.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi113315. 1 interaction.
STRINGi9606.ENSP00000381739.

PTM databases

PhosphoSiteiQ9H1J5.

Polymorphism and mutation databases

BioMutaiWNT8A.
DMDMi288558833.

2D gel databases

REPRODUCTION-2DPAGEQ9H1J5.

Proteomic databases

PaxDbiQ9H1J5.
PRIDEiQ9H1J5.

Protocols and materials databases

DNASUi7478.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000361560; ENSP00000354726; ENSG00000061492. [Q9H1J5-2]
ENST00000398754; ENSP00000381739; ENSG00000061492. [Q9H1J5-1]
GeneIDi7478.
KEGGihsa:7478.
UCSCiuc003lcd.1. human. [Q9H1J5-1]

Organism-specific databases

CTDi7478.
GeneCardsiGC05P137447.
HGNCiHGNC:12788. WNT8A.
HPAiHPA038539.
MIMi606360. gene.
neXtProtiNX_Q9H1J5.
PharmGKBiPA37389.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG277037.
GeneTreeiENSGT00690000101857.
HOGENOMiHOG000039529.
HOVERGENiHBG001595.
InParanoidiQ9H1J5.
KOiK00714.
OrthoDBiEOG7C8GJ8.
PhylomeDBiQ9H1J5.
TreeFamiTF105310.

Enzyme and pathway databases

ReactomeiREACT_163710. WNT ligand biogenesis and trafficking.
REACT_18372. Class B/2 (Secretin family receptors).
REACT_264034. disassembly of the destruction complex and recruitment of AXIN to the membrane.
REACT_264596. TCF dependent signaling in response to WNT.
SignaLinkiQ9H1J5.

Miscellaneous databases

GenomeRNAii7478.
NextBioi29292.
PROiQ9H1J5.
SOURCEiSearch...

Gene expression databases

BgeeiQ9H1J5.
CleanExiHS_WNT8A.
ExpressionAtlasiQ9H1J5. baseline and differential.
GenevisibleiQ9H1J5. HS.

Family and domain databases

InterProiIPR005817. Wnt.
IPR013301. Wnt8.
IPR018161. Wnt_CS.
[Graphical view]
PANTHERiPTHR12027. PTHR12027. 1 hit.
PTHR12027:SF92. PTHR12027:SF92. 1 hit.
PfamiPF00110. wnt. 1 hit.
[Graphical view]
PRINTSiPR01892. WNT8PROTEIN.
PR01349. WNTPROTEIN.
SMARTiSM00097. WNT1. 1 hit.
[Graphical view]
PROSITEiPS00246. WNT1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Molecular cloning and characterization of human WNT8A."
    Saitoh T., Katoh M.
    Int. J. Oncol. 19:123-127(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
  2. "Molecular cloning and characterization of six novel human WNT genes."
    Testa T.T., Mossakowska D.E., Carter P.S., Hu E., Zhu Y., Kelsell D.P., Murdock P.R., Herrity N.C., Lewis C.J., Cross D.A., Culbert A.A., Reith A.D., Barnes M.R.
    Submitted (AUG-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).

Entry informationi

Entry nameiWNT8A_HUMAN
AccessioniPrimary (citable) accession number: Q9H1J5
Secondary accession number(s): Q96S51
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 20, 2001
Last sequence update: February 9, 2010
Last modified: June 24, 2015
This is version 122 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 5
    Human chromosome 5: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.