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Protein

DNA-directed RNA polymerase III subunit RPC6

Gene

POLR3F

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific peripheric component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. May direct RNA Pol III binding to the TFIIIB-DNA complex. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway. Preferentially binds double-stranded DNA (dsDNA) (PubMed:21358628).3 Publications

GO - Molecular functioni

  • DNA-directed 5'-3' RNA polymerase activity Source: UniProtKB
  • double-stranded DNA binding Source: UniProtKB

GO - Biological processi

  • defense response to virus Source: UniProtKB-KW
  • innate immune response Source: UniProtKB-KW
  • positive regulation of innate immune response Source: UniProtKB
  • positive regulation of interferon-beta production Source: UniProtKB
  • regulation of transcription by RNA polymerase III Source: UniProtKB
  • transcription by RNA polymerase III Source: InterPro

Keywordsi

Molecular functionDNA-binding
Biological processAntiviral defense, Immunity, Innate immunity, Transcription

Enzyme and pathway databases

ReactomeiR-HSA-1834949 Cytosolic sensors of pathogen-associated DNA
R-HSA-73780 RNA Polymerase III Chain Elongation
R-HSA-73980 RNA Polymerase III Transcription Termination
R-HSA-749476 RNA Polymerase III Abortive And Retractive Initiation
R-HSA-76061 RNA Polymerase III Transcription Initiation From Type 1 Promoter
R-HSA-76066 RNA Polymerase III Transcription Initiation From Type 2 Promoter
R-HSA-76071 RNA Polymerase III Transcription Initiation From Type 3 Promoter

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-directed RNA polymerase III subunit RPC6
Short name:
RNA polymerase III subunit C6
Alternative name(s):
DNA-directed RNA polymerase III subunit F
RNA polymerase III 39 kDa subunit
Short name:
RPC39
Gene namesi
Name:POLR3F
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 20

Organism-specific databases

EuPathDBiHostDB:ENSG00000132664.11
HGNCiHGNC:15763 POLR3F
MIMi617455 gene
neXtProtiNX_Q9H1D9

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

DNA-directed RNA polymerase, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi137 – 140KAVK → EAVE: Strongly impaired dsDNA-binding. No effect on interaction with POLR3C. 1 Publication4
Mutagenesisi173 – 175ESE → KSK: Strongly impaired dsDNA-binding. No effect on interaction with POLR3C. 1 Publication3

Organism-specific databases

OpenTargetsiENSG00000132664
PharmGKBiPA33520

Polymorphism and mutation databases

DMDMi20139728

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00000739722 – 316DNA-directed RNA polymerase III subunit RPC6Add BLAST315

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1
Cross-linki5Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki7Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources

Keywords - PTMi

Acetylation, Isopeptide bond, Ubl conjugation

Proteomic databases

EPDiQ9H1D9
MaxQBiQ9H1D9
PaxDbiQ9H1D9
PeptideAtlasiQ9H1D9
PRIDEiQ9H1D9

PTM databases

iPTMnetiQ9H1D9
PhosphoSitePlusiQ9H1D9

Expressioni

Gene expression databases

BgeeiENSG00000132664
CleanExiHS_POLR3F
GenevisibleiQ9H1D9 HS

Organism-specific databases

HPAiHPA049441
HPA050173
HPA072298

Interactioni

Subunit structurei

Component of the RNA polymerase III (Pol III) complex consisting of 17 subunits. RPC3/POLR3C, RPC6/POLR3F and RPC7/POLR3G form a Pol III subcomplex (By similarity). Directly interacts with POLR3C (PubMed:21358628). Interacts with TBP and TFIIIB90 and GTF3C4 (By similarity) (PubMed:10523658).By similarity3 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi115866, 41 interactors
DIPiDIP-34646N
IntActiQ9H1D9, 33 interactors
MINTiQ9H1D9
STRINGi9606.ENSP00000366828

Structurei

Secondary structure

1316
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi73 – 76Combined sources4
Beta strandi79 – 81Combined sources3
Helixi92 – 102Combined sources11
Helixi109 – 115Combined sources7
Helixi120 – 132Combined sources13
Beta strandi135 – 139Combined sources5
Beta strandi149 – 155Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2DK5NMR-A78-155[»]
2YU3NMR-A61-142[»]
ProteinModelPortaliQ9H1D9
SMRiQ9H1D9
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9H1D9

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG3233 Eukaryota
COG5111 LUCA
GeneTreeiENSGT00390000009679
HOGENOMiHOG000231688
HOVERGENiHBG006817
InParanoidiQ9H1D9
KOiK03025
OMAiCEYMRDW
OrthoDBiEOG091G0DUX
PhylomeDBiQ9H1D9
TreeFamiTF103051

Family and domain databases

Gene3Di1.10.10.10, 2 hits
InterProiView protein in InterPro
IPR007832 RNA_pol_Rpc34
IPR016049 RNA_pol_Rpc34-like
IPR036388 WH-like_DNA-bd_sf
IPR036390 WH_DNA-bd_sf
PANTHERiPTHR12780 PTHR12780, 1 hit
PfamiView protein in Pfam
PF05158 RNA_pol_Rpc34, 1 hit
PIRSFiPIRSF028763 RNA_pol_Rpc34, 1 hit
SUPFAMiSSF46785 SSF46785, 2 hits

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9H1D9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAEVKVKVQP PDADPVEIEN RIIELCHQFP HGITDQVIQN EMPHIEAQQR
60 70 80 90 100
AVAINRLLSM GQLDLLRSNT GLLYRIKDSQ NAGKMKGSDN QEKLVYQIIE
110 120 130 140 150
DAGNKGIWSR DIRYKSNLPL TEINKILKNL ESKKLIKAVK SVAASKKKVY
160 170 180 190 200
MLYNLQPDRS VTGGAWYSDQ DFESEFVEVL NQQCFKFLQS KAETARESKQ
210 220 230 240 250
NPMIQRNSSF ASSHEVWKYI CELGISKVEL SMEDIETILN TLIYDGKVEM
260 270 280 290 300
TIIAAKEGTV GSVDGHMKLY RAVNPIIPPT GLVRAPCGLC PVFDDCHEGG
310
EISPSNCIYM TEWLEF
Length:316
Mass (Da):35,684
Last modified:March 1, 2001 - v1
Checksum:i49B1360AF8B365ED
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti2A → G in AAB63677 (PubMed:9171375).Curated1
Sequence conflicti44 – 53HIEAQQRAVA → QYRSPAAGSS in AAB63677 (PubMed:9171375).Curated10
Sequence conflicti255 – 290AKEGT…PCGLC → CKRRHSWQCRWTHETVQGSQ SNHPSHRFGPGHPVDSA in AAB63677 (PubMed:9171375).CuratedAdd BLAST36

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U93869 mRNA Translation: AAB63677.1
AK290892 mRNA Translation: BAF83581.1
AL121893 Genomic DNA No translation available.
CH471133 Genomic DNA Translation: EAX10240.1
BC012588 mRNA Translation: AAH12588.1
CCDSiCCDS13135.1
RefSeqiNP_001269455.1, NM_001282526.1
NP_006457.2, NM_006466.3
UniGeneiHs.472227

Genome annotation databases

EnsembliENST00000377603; ENSP00000366828; ENSG00000132664
GeneIDi10621
KEGGihsa:10621
UCSCiuc002wqv.5 human

Similar proteinsi

Entry informationi

Entry nameiRPC6_HUMAN
AccessioniPrimary (citable) accession number: Q9H1D9
Secondary accession number(s): A8K4C7, O15319
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 27, 2002
Last sequence update: March 1, 2001
Last modified: May 23, 2018
This is version 162 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

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