Reviewed, UniProtKB/Swiss-Prot Q9H1D9 (RPC6_HUMAN)

Names and origin

Protein names

Recommended name:
    DNA-directed RNA polymerase III subunit RPC6
      Short name=RNA polymerase III subunit C6
Alternative name(s):
    DNA-directed RNA polymerase III subunit F
    DNA-directed RNA polymerase III 39 kDa polypeptide
    RPC39

Gene names

Name:

POLR3F

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	316 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific peripheric component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. May direct RNA Pol III binding to the TFIIIB-DNA complex.
Subunit structure	Component of the RNA polymerase III (Pol III) complex consisting of 17 subunits. RPC3/POLR3C, RPC6/POLR3F and RPC7/POLR3G form a Pol III subcomplex By similarity. Interacts with TBP and TFIIIB90 and GTF3C4.
Subcellular location	Nucleus By similarity.
Sequence similarities	Belongs to the eukaryotic RPC34/RPC39 RNA polymerase subunit family.

Ontologies

Keywords
Biological process	Transcription
Cellular component	DNA-directed RNA polymerase Nucleus
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	regulation of transcription from RNA polymerase III promoter Ref.1 Non-traceable author statement. Source: UniProtKB transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	DNA-directed RNA polymerase III complex Non-traceable author statement. Source: UniProtKB
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed RNA polymerase activity Ref.1 Non-traceable author statement. Source: UniProtKB protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
PRPF4B	Q13523	1	EBI-710067,EBI-395940

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 316

316

DNA-directed RNA polymerase III subunit RPC6

PRO_0000073972

Experimental info

Sequence conflict

2

1

A → G in AAB63677. Ref.1

Sequence conflict

44 – 53

10

HIEAQQRAVA → QYRSPAAGSS in AAB63677. Ref.1

Sequence conflict

255 – 290

36

AKEGT…PCGLC → CKRRHSWQCRWTHETVQGSQ SNHPSHRFGPGHPVDSA Ref.1

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

316

Helix Strand Turn

Details...

Beta strand

73 – 76

4

Helix

92 – 102

11

Helix

109 – 115

7

Helix

120 – 132

13

Beta strand

135 – 139

5

Beta strand

149 – 155

7

Sequences

	Sequence		Length	Mass (Da)
	Q9H1D9-1 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: 49B1360AF8B365ED	FASTA	316	35,684
	10 20 30 40 50 60 MAEVKVKVQP PDADPVEIEN RIIELCHQFP HGITDQVIQN EMPHIEAQQR AVAINRLLSM 70 80 90 100 110 120 GQLDLLRSNT GLLYRIKDSQ NAGKMKGSDN QEKLVYQIIE DAGNKGIWSR DIRYKSNLPL 130 140 150 160 170 180 TEINKILKNL ESKKLIKAVK SVAASKKKVY MLYNLQPDRS VTGGAWYSDQ DFESEFVEVL 190 200 210 220 230 240 NQQCFKFLQS KAETARESKQ NPMIQRNSSF ASSHEVWKYI CELGISKVEL SMEDIETILN 250 260 270 280 290 300 TLIYDGKVEM TIIAAKEGTV GSVDGHMKLY RAVNPIIPPT GLVRAPCGLC PVFDDCHEGG 310 EISPSNCIYM TEWLEF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Three human RNA polymerase III-specific subunits form a subcomplex with a selective function in specific transcription initiation." Wang Z., Roeder R.G. Genes Dev. 11:1315-1326(1997) [PubMed: 9171375] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The DNA sequence and comparative analysis of human chromosome 20." Deloukas P., Matthews L.H., Ashurst J.L., Burton J., Gilbert J.G.R., Jones M., Stavrides G., Almeida J.P., Babbage A.K., Bagguley C.L., Bailey J., Barlow K.F., Bates K.N., Beard L.M., Beare D.M., Beasley O.P., Bird C.P., Blakey S.E. , Bridgeman A.M., Brown A.J., Buck D., Burrill W.D., Butler A.P., Carder C., Carter N.P., Chapman J.C., Clamp M., Clark G., Clark L.N., Clark S.Y., Clee C.M., Clegg S., Cobley V.E., Collier R.E., Connor R.E., Corby N.R., Coulson A., Coville G.J., Deadman R., Dhami P.D., Dunn M., Ellington A.G., Frankland J.A., Fraser A., French L., Garner P., Grafham D.V., Griffiths C., Griffiths M.N.D., Gwilliam R., Hall R.E., Hammond S., Harley J.L., Heath P.D., Ho S., Holden J.L., Howden P.J., Huckle E., Hunt A.R., Hunt S.E., Jekosch K., Johnson C.M., Johnson D., Kay M.P., Kimberley A.M., King A., Knights A., Laird G.K., Lawlor S., Lehvaeslaiho M.H., Leversha M.A., Lloyd C., Lloyd D.M., Lovell J.D., Marsh V.L., Martin S.L., McConnachie L.J., McLay K., McMurray A.A., Milne S.A., Mistry D., Moore M.J.F., Mullikin J.C., Nickerson T., Oliver K., Parker A., Patel R., Pearce T.A.V., Peck A.I., Phillimore B.J.C.T., Prathalingam S.R., Plumb R.W., Ramsay H., Rice C.M., Ross M.T., Scott C.E., Sehra H.K., Shownkeen R., Sims S., Skuce C.D., Smith M.L., Soderlund C., Steward C.A., Sulston J.E., Swann R.M., Sycamore N., Taylor R., Tee L., Thomas D.W., Thorpe A., Tracey A., Tromans A.C., Vaudin M., Wall M., Wallis J.M., Whitehead S.L., Whittaker P., Willey D.L., Williams L., Williams S.A., Wilming L., Wray P.W., Hubbard T., Durbin R.M., Bentley D.R., Beck S., Rogers J. Nature 414:865-871(2001) [PubMed: 11780052] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[4]	"The TFIIIC90 subunit of TFIIIC interacts with multiple components of the RNA polymerase III machinery and contains a histone-specific acetyltransferase activity." Hsieh Y.-J., Kundu T.K., Wang Z., Kovelman R., Roeder R.G. Mol. Cell. Biol. 19:7697-7704(1999) [PubMed: 10523658] [Abstract] Cited for: INTERACTION WITH GTF3C4.
[5]	"Characterization of human RNA polymerase III identifies orthologues for Saccharomyces cerevisiae RNA polymerase III subunits." Hu P., Wu S., Sun Y., Yuan C.-C., Kobayashi R., Myers M.P., Hernandez N. Mol. Cell. Biol. 22:8044-8055(2002) [PubMed: 12391170] [Abstract] Cited for: IDENTIFICATION IN THE RNA POL III COMPLEX, MASS SPECTROMETRY.
[6]	Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
[7]	"Solution structure of winged-helix domain in RNA polymerase III 39kDa polypeptide." RIKEN structural genomics initiative (RSGI) Submitted (OCT-2006) to the PDB data bank Cited for: STRUCTURE BY NMR OF 78-155.

Cross-references

Sequence databases

EMBLGenBankDDBJ

U93869 mRNA. Translation: AAB63677.1.
AL121893 Genomic DNA. Translation: CAC11110.1.
BC012588 mRNA. Translation: AAH12588.1.

IPI

IPI00100245.

RefSeq

NP_006457.2.

UniGene

Hs.472227

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2DK5	NMR	-	A	78-155	[»]
2YU3	NMR	-	A	59-143	[»]

SMR

Q9H1D9. Positions 11-79.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q9H1D9. 6 interactions.

PTM databases

PhosphoSite

Q9H1D9.

Proteomic databases

PRIDE

Q9H1D9.

Genome annotation databases

Ensembl

ENSG00000132664. Homo sapiens. [Contig view]

GeneID

10621.

KEGG

hsa:10621.

UCSC

uc002wqv.1. human.

Organism-specific databases

GeneCards

GC20P018395.

H-InvDB

HIX0015667.

HGNC

HGNC:15763. POLR3F.

PharmGKB

PA33520.

GenAtlas

Search...

Phylogenomic databases

HOGENOM

Q9H1D9.

HOVERGEN

Q9H1D9.

OMA

Q9H1D9. TPCGLCP.

Enzyme and pathway databases

Reactome

REACT_1788. Transcription.

Gene expression databases

ArrayExpress

Q9H1D9.

Bgee

Q9H1D9.

CleanEx

HS_POLR3F.

GermOnline

ENSG00000132664. Homo sapiens.

Family and domain databases

InterPro

IPR007832. RNA_pol_Rpc34.
IPR016049. RNA_pol_Rpc34-like.
[Graphical view]

PANTHER

PTHR12780. RNA_pol_Rpc34. 1 hit.

Pfam

PF05158. RNA_pol_Rpc34. 1 hit.
[Graphical view]

PIRSF

PIRSF028763. RNA_pol_Rpc34. 1 hit.

ProtoNet

Search...

Other Resources

NextBio

40356.

Entry information

Entry name

RPC6_HUMAN

Accession

Primary (citable) accession number: Q9H1D9
Secondary accession number(s): O15319

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 27, 2002
Last sequence update:	March 1, 2001
Last modified:	July 7, 2009
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)