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Protein

Nuclear RNA export factor 5

Gene

NXF5

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Could be involved in the export of mRNA from the nucleus to the cytoplasm. Could also have a role in polarized cytoplasmic transport and localization of mRNA in neurons.

GO - Molecular functioni

  • nucleotide binding Source: InterPro
  • poly(A) RNA binding Source: GO_Central
  • RNA binding Source: UniProtKB

GO - Biological processi

  • mRNA export from nucleus Source: UniProtKB
  • multicellular organism development Source: UniProtKB
  • poly(A)+ mRNA export from nucleus Source: GO_Central
  • RNA transport Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

mRNA transport, Transport

Keywords - Ligandi

RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Nuclear RNA export factor 5
Alternative name(s):
TAP-like protein 1
Short name:
TAPL-1
Gene namesi
Name:NXF5
Synonyms:TAPL1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome X

Organism-specific databases

HGNCiHGNC:8075. NXF5.

Subcellular locationi

  • Cytoplasm
  • Nucleus

  • Note: Mainly localized in the cytoplasm of cells and more particularly in the cell body and neurites of hippocampal neurons. Although nuclear localization is also observed. Not detected at nuclear rim.

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Involvement in diseasei

A chromosomal aberration involving NXF5 has been observed in one patient with a syndromic form of mental retardation and short stature. Pericentric inversion inv(X)(p21.1;q22) that interrupts NXF5.

Organism-specific databases

PharmGKBiPA31862.

Polymorphism and mutation databases

BioMutaiNXF5.
DMDMi20978537.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 397397Nuclear RNA export factor 5PRO_0000220535Add
BLAST

Proteomic databases

PaxDbiQ9H1B4.
PRIDEiQ9H1B4.

PTM databases

PhosphoSiteiQ9H1B4.

Expressioni

Gene expression databases

BgeeiQ9H1B4.
CleanExiHS_NXF5.
GenevisibleiQ9H1B4. HS.

Organism-specific databases

HPAiHPA002079.

Interactioni

Subunit structurei

Interacts with NXT1 and NXT2.

Protein-protein interaction databases

BioGridi121023. 8 interactions.
STRINGi9606.ENSP00000426978.

Structurei

3D structure databases

ProteinModelPortaliQ9H1B4.
SMRiQ9H1B4. Positions 13-351.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini13 – 9280RRMAdd
BLAST
Repeati160 – 18526LRR 1Add
BLAST
Repeati186 – 20924LRR 2Add
BLAST
Repeati210 – 23728LRR 3Add
BLAST
Repeati238 – 26528LRR 4Add
BLAST
Domaini280 – 36788NTF2; truncatedAdd
BLAST

Domaini

The NTF2 domain heterodimerizes with NXT1 and NXT2.By similarity
The RNA-binding domain is a non-canonical RNP-type domain.

Sequence similaritiesi

Belongs to the NXF family.Curated
Contains 4 LRR (leucine-rich) repeats.Curated
Contains 1 NTF2 domain.Curated

Keywords - Domaini

Leucine-rich repeat, Repeat

Phylogenomic databases

eggNOGiKOG3763. Eukaryota.
ENOG410XR55. LUCA.
GeneTreeiENSGT00390000007539.
HOGENOMiHOG000236269.
HOVERGENiHBG013199.
InParanoidiQ9H1B4.
KOiK14284.
OMAiFTAPYSV.
OrthoDBiEOG7QZGB9.
PhylomeDBiQ9H1B4.
TreeFamiTF314566.

Family and domain databases

Gene3Di3.10.450.50. 1 hit.
3.30.70.330. 1 hit.
3.80.10.10. 1 hit.
InterProiIPR032675. L_dom-like.
IPR001611. Leu-rich_rpt.
IPR032710. NTF2-like_dom.
IPR012677. Nucleotide-bd_a/b_plait.
IPR030218. NXF5.
IPR030217. NXF_fam.
IPR015245. Tap_RNA-bd.
[Graphical view]
PANTHERiPTHR10662. PTHR10662. 1 hit.
PTHR10662:SF26. PTHR10662:SF26. 1 hit.
PfamiPF09162. Tap-RNA_bind. 1 hit.
[Graphical view]
ProDomiPD043466. Tap_RNA_bd. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF52058. SSF52058. 1 hit.
SSF54427. SSF54427. 1 hit.
SSF54928. SSF54928. 1 hit.
PROSITEiPS51450. LRR. 3 hits.
[Graphical view]

Sequences (6)i

Sequence statusi: Complete.

This entry describes 6 isoformsi produced by alternative splicing. AlignAdd to basket

Note: Additional isoforms seem to exist.

Isoform A (identifier: Q9H1B4-1) [UniParc]FASTAAdd to basket

Also known as: TAPL-1a

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRRNTQDENM RKWFKVTIPY GIKYDKAWLM NSIQSNCSVP FTPVDFHYIR
60 70 80 90 100
NRACFFVQVA SAASALKDVS YKIYDDENQK ICIFVSHFTA PYSVKNKLKP
110 120 130 140 150
GQMEMLKLTM NKRYNVSQQA LDLQNLRFDP DLMGRDIDII LNRRNCMAAT
160 170 180 190 200
LKITERNFPE LLSLNLCNNK LYQLDGLSDI TEKAPKVKTL NLSKNKLESA
210 220 230 240 250
WELGKVKGLK LEELWLEGNP LCSTFSDQSA YVSAIRDCFP KLLRLDGREL
260 270 280 290 300
SAPVIVDIDS SETMKPCKEN FTGSETLKHL VLQFLQQSNL CKYFKDSRNI
310 320 330 340 350
KILKDPYLQR KLLKHTKCPR NVDSLSALPE TQHDFTSILV DMWYQTVNTC
360 370 380 390
FLPRAGPESQ RWWCLLSLKW KDGLRVLILP SCGPSSLPLA AIPVCAS
Note: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.
Length:397
Mass (Da):45,628
Last modified:March 1, 2001 - v1
Checksum:i0D5E5EDCA850E3A6
GO
Isoform B (identifier: Q9H1B4-2) [UniParc]FASTAAdd to basket

Also known as: TAPL-1b

The sequence of this isoform differs from the canonical sequence as follows:
     361-368: RWWCLLSL → SGRMVSGF
     369-397: Missing.

Note: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.
Show »
Length:368
Mass (Da):42,417
Checksum:i19C121BB5CCAFD98
GO
Isoform C (identifier: Q9H1B4-3) [UniParc]FASTAAdd to basket

Also known as: TAPL-1c

The sequence of this isoform differs from the canonical sequence as follows:
     161-168: LLSLNLCN → RRLPKSRP
     169-397: Missing.

Note: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.
Show »
Length:168
Mass (Da):19,824
Checksum:i2E79418A641AE3FA
GO
Isoform D (identifier: Q9H1B4-4) [UniParc]FASTAAdd to basket

Also known as: TAPL-1d

The sequence of this isoform differs from the canonical sequence as follows:
     18-80: Missing.
     361-368: RWWCLLSL → SGRMVSGF
     369-397: Missing.

Note: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.
Show »
Length:305
Mass (Da):35,167
Checksum:i55160EF749700797
GO
Isoform E (identifier: Q9H1B4-5) [UniParc]FASTAAdd to basket

Also known as: TAPL-1e

The sequence of this isoform differs from the canonical sequence as follows:
     18-80: Missing.
     363-365: WCL → VGG
     366-397: Missing.

Note: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.
Show »
Length:302
Mass (Da):34,901
Checksum:i7DF796DC969D320C
GO
Isoform F (identifier: Q9H1B4-6) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     361-397: RWWCLLSLKWKDGLRVLILPSCGPSSLPLAAIPVCAS → SLRPL

Show »
Length:365
Mass (Da):42,162
Checksum:i3EAD3AF2C8555ADC
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei18 – 8063Missing in isoform D and isoform E. 1 PublicationVSP_003731Add
BLAST
Alternative sequencei161 – 1688LLSLNLCN → RRLPKSRP in isoform C. 1 PublicationVSP_003732
Alternative sequencei169 – 397229Missing in isoform C. 1 PublicationVSP_003733Add
BLAST
Alternative sequencei361 – 39737RWWCL…PVCAS → SLRPL in isoform F. 1 PublicationVSP_043896Add
BLAST
Alternative sequencei361 – 3688RWWCLLSL → SGRMVSGF in isoform B and isoform D. 1 PublicationVSP_003734
Alternative sequencei363 – 3653WCL → VGG in isoform E. 1 PublicationVSP_003735
Alternative sequencei366 – 39732Missing in isoform E. 1 PublicationVSP_003736Add
BLAST
Alternative sequencei369 – 39729Missing in isoform D and isoform B. 1 PublicationVSP_003737Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ277654 mRNA. Translation: CAC20428.1.
AJ277655 mRNA. Translation: CAC20429.1.
AJ277656 mRNA. Translation: CAC20430.1.
AJ277657 mRNA. Translation: CAC20431.1.
AJ277658 mRNA. Translation: CAC20432.1.
AL672207, Z70228 Genomic DNA. Translation: CAI42476.1.
AL672207, Z70228 Genomic DNA. Translation: CAI42478.1.
AL672207, Z70228 Genomic DNA. Translation: CAI42475.1.
AL672207, Z70228 Genomic DNA. Translation: CAI42477.1.
BC131708 mRNA. Translation: AAI31709.1.
CCDSiCCDS14491.2. [Q9H1B4-6]
RefSeqiNP_116564.2. NM_032946.2. [Q9H1B4-6]
XP_011529294.1. XM_011530992.1. [Q9H1B4-2]
UniGeneiHs.307077.

Genome annotation databases

EnsembliENST00000263032; ENSP00000263032; ENSG00000126952. [Q9H1B4-1]
ENST00000332614; ENSP00000331535; ENSG00000126952. [Q9H1B4-5]
ENST00000361330; ENSP00000354539; ENSG00000126952. [Q9H1B4-4]
ENST00000372803; ENSP00000361889; ENSG00000126952. [Q9H1B4-2]
ENST00000473265; ENSP00000426978; ENSG00000126952. [Q9H1B4-6]
ENST00000493509; ENSP00000422976; ENSG00000126952. [Q9H1B4-3]
ENST00000537026; ENSP00000442401; ENSG00000126952. [Q9H1B4-1]
GeneIDi55998.
KEGGihsa:55998.
UCSCiuc004eih.2. human. [Q9H1B4-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ277654 mRNA. Translation: CAC20428.1.
AJ277655 mRNA. Translation: CAC20429.1.
AJ277656 mRNA. Translation: CAC20430.1.
AJ277657 mRNA. Translation: CAC20431.1.
AJ277658 mRNA. Translation: CAC20432.1.
AL672207, Z70228 Genomic DNA. Translation: CAI42476.1.
AL672207, Z70228 Genomic DNA. Translation: CAI42478.1.
AL672207, Z70228 Genomic DNA. Translation: CAI42475.1.
AL672207, Z70228 Genomic DNA. Translation: CAI42477.1.
BC131708 mRNA. Translation: AAI31709.1.
CCDSiCCDS14491.2. [Q9H1B4-6]
RefSeqiNP_116564.2. NM_032946.2. [Q9H1B4-6]
XP_011529294.1. XM_011530992.1. [Q9H1B4-2]
UniGeneiHs.307077.

3D structure databases

ProteinModelPortaliQ9H1B4.
SMRiQ9H1B4. Positions 13-351.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi121023. 8 interactions.
STRINGi9606.ENSP00000426978.

PTM databases

PhosphoSiteiQ9H1B4.

Polymorphism and mutation databases

BioMutaiNXF5.
DMDMi20978537.

Proteomic databases

PaxDbiQ9H1B4.
PRIDEiQ9H1B4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000263032; ENSP00000263032; ENSG00000126952. [Q9H1B4-1]
ENST00000332614; ENSP00000331535; ENSG00000126952. [Q9H1B4-5]
ENST00000361330; ENSP00000354539; ENSG00000126952. [Q9H1B4-4]
ENST00000372803; ENSP00000361889; ENSG00000126952. [Q9H1B4-2]
ENST00000473265; ENSP00000426978; ENSG00000126952. [Q9H1B4-6]
ENST00000493509; ENSP00000422976; ENSG00000126952. [Q9H1B4-3]
ENST00000537026; ENSP00000442401; ENSG00000126952. [Q9H1B4-1]
GeneIDi55998.
KEGGihsa:55998.
UCSCiuc004eih.2. human. [Q9H1B4-1]

Organism-specific databases

CTDi55998.
GeneCardsiNXF5.
H-InvDBHIX0056181.
HGNCiHGNC:8075. NXF5.
HPAiHPA002079.
MIMi300319. gene.
neXtProtiNX_Q9H1B4.
PharmGKBiPA31862.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3763. Eukaryota.
ENOG410XR55. LUCA.
GeneTreeiENSGT00390000007539.
HOGENOMiHOG000236269.
HOVERGENiHBG013199.
InParanoidiQ9H1B4.
KOiK14284.
OMAiFTAPYSV.
OrthoDBiEOG7QZGB9.
PhylomeDBiQ9H1B4.
TreeFamiTF314566.

Miscellaneous databases

GenomeRNAii55998.
NextBioi61439.
PROiQ9H1B4.
SOURCEiSearch...

Gene expression databases

BgeeiQ9H1B4.
CleanExiHS_NXF5.
GenevisibleiQ9H1B4. HS.

Family and domain databases

Gene3Di3.10.450.50. 1 hit.
3.30.70.330. 1 hit.
3.80.10.10. 1 hit.
InterProiIPR032675. L_dom-like.
IPR001611. Leu-rich_rpt.
IPR032710. NTF2-like_dom.
IPR012677. Nucleotide-bd_a/b_plait.
IPR030218. NXF5.
IPR030217. NXF_fam.
IPR015245. Tap_RNA-bd.
[Graphical view]
PANTHERiPTHR10662. PTHR10662. 1 hit.
PTHR10662:SF26. PTHR10662:SF26. 1 hit.
PfamiPF09162. Tap-RNA_bind. 1 hit.
[Graphical view]
ProDomiPD043466. Tap_RNA_bd. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF52058. SSF52058. 1 hit.
SSF54427. SSF54427. 1 hit.
SSF54928. SSF54928. 1 hit.
PROSITEiPS51450. LRR. 3 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "NXF5, a novel member of the nuclear RNA export factor family, is lost in a male patient with a syndromic form of mental retardation."
    Jun L., Frints S., Duhamel H., Herold A., Abad-Rodrigues J., Dotti C., Izaurralde E., Marynen P., Froyen G.
    Curr. Biol. 11:1381-1391(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS A; B; C; D AND E), CHROMOSOMAL INVERSION.
    Tissue: Fetal brain.
  2. "The DNA sequence of the human X chromosome."
    Ross M.T., Grafham D.V., Coffey A.J., Scherer S., McLay K., Muzny D., Platzer M., Howell G.R., Burrows C., Bird C.P., Frankish A., Lovell F.L., Howe K.L., Ashurst J.L., Fulton R.S., Sudbrak R., Wen G., Jones M.C.
    , Hurles M.E., Andrews T.D., Scott C.E., Searle S., Ramser J., Whittaker A., Deadman R., Carter N.P., Hunt S.E., Chen R., Cree A., Gunaratne P., Havlak P., Hodgson A., Metzker M.L., Richards S., Scott G., Steffen D., Sodergren E., Wheeler D.A., Worley K.C., Ainscough R., Ambrose K.D., Ansari-Lari M.A., Aradhya S., Ashwell R.I., Babbage A.K., Bagguley C.L., Ballabio A., Banerjee R., Barker G.E., Barlow K.F., Barrett I.P., Bates K.N., Beare D.M., Beasley H., Beasley O., Beck A., Bethel G., Blechschmidt K., Brady N., Bray-Allen S., Bridgeman A.M., Brown A.J., Brown M.J., Bonnin D., Bruford E.A., Buhay C., Burch P., Burford D., Burgess J., Burrill W., Burton J., Bye J.M., Carder C., Carrel L., Chako J., Chapman J.C., Chavez D., Chen E., Chen G., Chen Y., Chen Z., Chinault C., Ciccodicola A., Clark S.Y., Clarke G., Clee C.M., Clegg S., Clerc-Blankenburg K., Clifford K., Cobley V., Cole C.G., Conquer J.S., Corby N., Connor R.E., David R., Davies J., Davis C., Davis J., Delgado O., Deshazo D., Dhami P., Ding Y., Dinh H., Dodsworth S., Draper H., Dugan-Rocha S., Dunham A., Dunn M., Durbin K.J., Dutta I., Eades T., Ellwood M., Emery-Cohen A., Errington H., Evans K.L., Faulkner L., Francis F., Frankland J., Fraser A.E., Galgoczy P., Gilbert J., Gill R., Gloeckner G., Gregory S.G., Gribble S., Griffiths C., Grocock R., Gu Y., Gwilliam R., Hamilton C., Hart E.A., Hawes A., Heath P.D., Heitmann K., Hennig S., Hernandez J., Hinzmann B., Ho S., Hoffs M., Howden P.J., Huckle E.J., Hume J., Hunt P.J., Hunt A.R., Isherwood J., Jacob L., Johnson D., Jones S., de Jong P.J., Joseph S.S., Keenan S., Kelly S., Kershaw J.K., Khan Z., Kioschis P., Klages S., Knights A.J., Kosiura A., Kovar-Smith C., Laird G.K., Langford C., Lawlor S., Leversha M., Lewis L., Liu W., Lloyd C., Lloyd D.M., Loulseged H., Loveland J.E., Lovell J.D., Lozado R., Lu J., Lyne R., Ma J., Maheshwari M., Matthews L.H., McDowall J., McLaren S., McMurray A., Meidl P., Meitinger T., Milne S., Miner G., Mistry S.L., Morgan M., Morris S., Mueller I., Mullikin J.C., Nguyen N., Nordsiek G., Nyakatura G., O'dell C.N., Okwuonu G., Palmer S., Pandian R., Parker D., Parrish J., Pasternak S., Patel D., Pearce A.V., Pearson D.M., Pelan S.E., Perez L., Porter K.M., Ramsey Y., Reichwald K., Rhodes S., Ridler K.A., Schlessinger D., Schueler M.G., Sehra H.K., Shaw-Smith C., Shen H., Sheridan E.M., Shownkeen R., Skuce C.D., Smith M.L., Sotheran E.C., Steingruber H.E., Steward C.A., Storey R., Swann R.M., Swarbreck D., Tabor P.E., Taudien S., Taylor T., Teague B., Thomas K., Thorpe A., Timms K., Tracey A., Trevanion S., Tromans A.C., d'Urso M., Verduzco D., Villasana D., Waldron L., Wall M., Wang Q., Warren J., Warry G.L., Wei X., West A., Whitehead S.L., Whiteley M.N., Wilkinson J.E., Willey D.L., Williams G., Williams L., Williamson A., Williamson H., Wilming L., Woodmansey R.L., Wray P.W., Yen J., Zhang J., Zhou J., Zoghbi H., Zorilla S., Buck D., Reinhardt R., Poustka A., Rosenthal A., Lehrach H., Meindl A., Minx P.J., Hillier L.W., Willard H.F., Wilson R.K., Waterston R.H., Rice C.M., Vaudin M., Coulson A., Nelson D.L., Weinstock G., Sulston J.E., Durbin R.M., Hubbard T., Gibbs R.A., Beck S., Rogers J., Bentley D.R.
    Nature 434:325-337(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM F).
  4. Cited for: SPLICE ISOFORM(S) THAT ARE POTENTIAL NMD TARGET(S).

Entry informationi

Entry nameiNXF5_HUMAN
AccessioniPrimary (citable) accession number: Q9H1B4
Secondary accession number(s): A2RRM0
, B1AV82, B1AV83, B1AV84, B1AV85, Q9H1B0, Q9H1B1, Q9H1B2, Q9H1B3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 15, 2002
Last sequence update: March 1, 2001
Last modified: May 11, 2016
This is version 136 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome X
    Human chromosome X: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.