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Protein

Inhibitor of growth protein 2

Gene

ING2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Seems to be involved in p53/TP53 activation and p53/TP53-dependent apoptotic pathways, probably by enhancing acetylation of p53/TP53. Component of a mSin3A-like corepressor complex, which is probably involved in deacetylation of nucleosomal histones. ING2 activity seems to be modulated by binding to phosphoinositides (PtdInsPs).2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei214Histone H3K4me3By similarity1
Metal bindingi215Zinc 1By similarity1
Metal bindingi217Zinc 1By similarity1
Binding sitei225Histone H3K4me3By similarity1
Metal bindingi228Zinc 2By similarity1
Binding sitei229Histone H3K4me3By similarity1
Metal bindingi233Zinc 2By similarity1
Binding sitei237Histone H3K4me3By similarity1
Metal bindingi239Zinc 1; via pros nitrogenBy similarity1
Metal bindingi242Zinc 1By similarity1
Metal bindingi255Zinc 2By similarity1
Metal bindingi258Zinc 2By similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri212 – 261PHD-typePROSITE-ProRule annotationAdd BLAST50

GO - Molecular functioni

  • chromatin binding Source: ProtInc
  • DNA binding Source: UniProtKB
  • methylated histone binding Source: UniProtKB
  • phosphatidylinositol binding Source: UniProtKB
  • protein complex binding Source: UniProtKB
  • zinc ion binding Source: InterPro

GO - Biological processi

  • chromatin modification Source: UniProtKB-KW
  • flagellated sperm motility Source: BHF-UCL
  • male germ-line stem cell asymmetric division Source: BHF-UCL
  • male meiosis I Source: BHF-UCL
  • negative regulation of cell proliferation Source: InterPro
  • negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator Source: BHF-UCL
  • positive regulation of histone deacetylation Source: BHF-UCL
  • positive regulation of transcription, DNA-templated Source: UniProtKB
  • positive regulation of transforming growth factor beta receptor signaling pathway Source: UniProtKB
  • regulation of cellular senescence Source: BHF-UCL
  • regulation of growth Source: UniProtKB-KW
  • regulation of response to DNA damage stimulus Source: BHF-UCL
  • regulation of transcription, DNA-templated Source: UniProtKB
  • seminiferous tubule development Source: BHF-UCL
  • signal transduction Source: ProtInc
  • spermatid development Source: BHF-UCL
  • spermatogenesis Source: BHF-UCL
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Chromatin regulator

Keywords - Biological processi

Growth regulation, Transcription, Transcription regulation

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-HSA-6811555. PI5P Regulates TP53 Acetylation.

Names & Taxonomyi

Protein namesi
Recommended name:
Inhibitor of growth protein 2
Alternative name(s):
Inhibitor of growth 1-like protein
Short name:
ING1Lp
p32
p33ING2
Gene namesi
Name:ING2
Synonyms:ING1L
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 4

Organism-specific databases

HGNCiHGNC:6063. ING2.

Subcellular locationi

GO - Cellular componenti

  • CCAAT-binding factor complex Source: UniProtKB
  • cytoplasm Source: HPA
  • Golgi apparatus Source: HPA
  • nucleoplasm Source: HPA
  • nucleus Source: UniProtKB
  • plasma membrane Source: ParkinsonsUK-UCL
  • Sin3 complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi3622.
OpenTargetsiENSG00000168556.
PharmGKBiPA29873.

Polymorphism and mutation databases

BioMutaiING2.
DMDMi59798471.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002126631 – 280Inhibitor of growth protein 2Add BLAST280

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki195Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1)1 Publication

Post-translational modificationi

Sumoylation enhances its association with SIN3A and is required for binding to some target gene promoters, this is the case for TMEM71.1 Publication

Keywords - PTMi

Isopeptide bond, Ubl conjugation

Proteomic databases

EPDiQ9H160.
MaxQBiQ9H160.
PaxDbiQ9H160.
PeptideAtlasiQ9H160.
PRIDEiQ9H160.

PTM databases

iPTMnetiQ9H160.
PhosphoSitePlusiQ9H160.

Expressioni

Tissue specificityi

Widely expressed. Higher expressed in colon-cancer tumor than in normal colon tissues.1 Publication

Inductioni

Induced by the DNA-damaging agents etoposide and neocarzinostatin.1 Publication

Gene expression databases

BgeeiENSG00000168556.
CleanExiHS_ING2.
ExpressionAtlasiQ9H160. baseline and differential.
GenevisibleiQ9H160. HS.

Organism-specific databases

HPAiHPA019486.
HPA021517.

Interactioni

Subunit structurei

Interacts with H3K4me3 and to a lesser extent with H3K4me2. Component of a mSin3A-like complex at least consisting of SIN3A, HDAC1, HDAC2, RBBP4/RbAp48, RBBP7/RbAp46, SAP30 and ING2.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
PCNAP120043EBI-389787,EBI-358311
SMURF1Q9HCE7-23EBI-389787,EBI-9845742

GO - Molecular functioni

  • methylated histone binding Source: UniProtKB
  • protein complex binding Source: UniProtKB

Protein-protein interaction databases

BioGridi109834. 44 interactors.
IntActiQ9H160. 12 interactors.
MINTiMINT-2830779.
STRINGi9606.ENSP00000307183.

Structurei

3D structure databases

ProteinModelPortaliQ9H160.
SMRiQ9H160.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni264 – 280PBR1 PublicationAdd BLAST17

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili48 – 120Sequence analysisAdd BLAST73

Domaini

The PHD-type zinc finger mediates the binding to H3K4me3.1 Publication
The polybasic region (PBR) is responsive to the binding to phosphoinositides (PtdInsPs), including phosphatidylinositol 5-phosphate (PtdIns5P).1 Publication

Sequence similaritiesi

Belongs to the ING family.Curated
Contains 1 PHD-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri212 – 261PHD-typePROSITE-ProRule annotationAdd BLAST50

Keywords - Domaini

Coiled coil, Zinc-finger

Phylogenomic databases

eggNOGiKOG1973. Eukaryota.
COG5034. LUCA.
GeneTreeiENSGT00550000074538.
HOGENOMiHOG000239724.
HOVERGENiHBG006607.
InParanoidiQ9H160.
KOiK19198.
OMAiLCHMTNG.
OrthoDBiEOG091G0J8Y.
PhylomeDBiQ9H160.
TreeFamiTF352014.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR028639. ING2.
IPR028651. ING_fam.
IPR024610. ING_N_histone_binding.
IPR019786. Zinc_finger_PHD-type_CS.
IPR011011. Znf_FYVE_PHD.
IPR001965. Znf_PHD.
IPR019787. Znf_PHD-finger.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PANTHERiPTHR10333. PTHR10333. 1 hit.
PTHR10333:SF37. PTHR10333:SF37. 1 hit.
PfamiPF12998. ING. 1 hit.
[Graphical view]
SMARTiSM01408. ING. 1 hit.
SM00249. PHD. 1 hit.
[Graphical view]
SUPFAMiSSF57903. SSF57903. 1 hit.
PROSITEiPS01359. ZF_PHD_1. 1 hit.
PS50016. ZF_PHD_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform ING2a (identifier: Q9H160-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLGQQQQQLY SSAALLTGER SRLLTCYVQD YLECVESLPH DMQRNVSVLR
60 70 80 90 100
ELDNKYQETL KEIDDVYEKY KKEDDLNQKK RLQQLLQRAL INSQELGDEK
110 120 130 140 150
IQIVTQMLEL VENRARQMEL HSQCFQDPAE SERASDKAKM DSSQPERSSR
160 170 180 190 200
RPRRQRTSES RDLCHMANGI EDCDDQPPKE KKSKSAKKKK RSKAKQEREA
210 220 230 240 250
SPVEFAIDPN EPTYCLCNQV SYGEMIGCDN EQCPIEWFHF SCVSLTYKPK
260 270 280
GKWYCPKCRG DNEKTMDKST EKTKKDRRSR
Length:280
Mass (Da):32,808
Last modified:February 15, 2005 - v2
Checksum:iDC85A6ECAF7A5D81
GO
Isoform ING2b (identifier: Q9H160-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-58: MLGQQQQQLY...LRELDNKYQE → MDQDGDQQLGPSRILAPQ

Note: Low expression except in testis, where it reaches half of ING2a levels.
Show »
Length:240
Mass (Da):27,984
Checksum:iA445AEC5BBFA3B35
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti21S → T in CAC20567 (Ref. 4) Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0478211 – 58MLGQQ…NKYQE → MDQDGDQQLGPSRILAPQ in isoform ING2b. 2 PublicationsAdd BLAST58

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB012853 mRNA. Translation: BAA36419.1.
AF053537 mRNA. Translation: AAG11395.1.
AF062748, AF062747 Genomic DNA. Translation: AAG11396.1.
AB196793 mRNA. Translation: BAF30476.1.
AJ006851 mRNA. Translation: CAC20567.1.
AK294310 mRNA. Translation: BAH11731.1.
AC107214 Genomic DNA. No translation available.
BC030128 mRNA. Translation: AAH30128.1.
CCDSiCCDS3833.1. [Q9H160-1]
RefSeqiNP_001278888.1. NM_001291959.1. [Q9H160-2]
NP_001555.1. NM_001564.3. [Q9H160-1]
UniGeneiHs.107153.

Genome annotation databases

EnsembliENST00000302327; ENSP00000307183; ENSG00000168556. [Q9H160-1]
GeneIDi3622.
KEGGihsa:3622.
UCSCiuc003ivs.2. human. [Q9H160-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Web resourcesi

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB012853 mRNA. Translation: BAA36419.1.
AF053537 mRNA. Translation: AAG11395.1.
AF062748, AF062747 Genomic DNA. Translation: AAG11396.1.
AB196793 mRNA. Translation: BAF30476.1.
AJ006851 mRNA. Translation: CAC20567.1.
AK294310 mRNA. Translation: BAH11731.1.
AC107214 Genomic DNA. No translation available.
BC030128 mRNA. Translation: AAH30128.1.
CCDSiCCDS3833.1. [Q9H160-1]
RefSeqiNP_001278888.1. NM_001291959.1. [Q9H160-2]
NP_001555.1. NM_001564.3. [Q9H160-1]
UniGeneiHs.107153.

3D structure databases

ProteinModelPortaliQ9H160.
SMRiQ9H160.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi109834. 44 interactors.
IntActiQ9H160. 12 interactors.
MINTiMINT-2830779.
STRINGi9606.ENSP00000307183.

PTM databases

iPTMnetiQ9H160.
PhosphoSitePlusiQ9H160.

Polymorphism and mutation databases

BioMutaiING2.
DMDMi59798471.

Proteomic databases

EPDiQ9H160.
MaxQBiQ9H160.
PaxDbiQ9H160.
PeptideAtlasiQ9H160.
PRIDEiQ9H160.

Protocols and materials databases

DNASUi3622.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000302327; ENSP00000307183; ENSG00000168556. [Q9H160-1]
GeneIDi3622.
KEGGihsa:3622.
UCSCiuc003ivs.2. human. [Q9H160-1]

Organism-specific databases

CTDi3622.
DisGeNETi3622.
GeneCardsiING2.
HGNCiHGNC:6063. ING2.
HPAiHPA019486.
HPA021517.
MIMi604215. gene.
neXtProtiNX_Q9H160.
OpenTargetsiENSG00000168556.
PharmGKBiPA29873.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1973. Eukaryota.
COG5034. LUCA.
GeneTreeiENSGT00550000074538.
HOGENOMiHOG000239724.
HOVERGENiHBG006607.
InParanoidiQ9H160.
KOiK19198.
OMAiLCHMTNG.
OrthoDBiEOG091G0J8Y.
PhylomeDBiQ9H160.
TreeFamiTF352014.

Enzyme and pathway databases

ReactomeiR-HSA-6811555. PI5P Regulates TP53 Acetylation.

Miscellaneous databases

ChiTaRSiING2. human.
GeneWikiiING2.
GenomeRNAii3622.
PROiQ9H160.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000168556.
CleanExiHS_ING2.
ExpressionAtlasiQ9H160. baseline and differential.
GenevisibleiQ9H160. HS.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR028639. ING2.
IPR028651. ING_fam.
IPR024610. ING_N_histone_binding.
IPR019786. Zinc_finger_PHD-type_CS.
IPR011011. Znf_FYVE_PHD.
IPR001965. Znf_PHD.
IPR019787. Znf_PHD-finger.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PANTHERiPTHR10333. PTHR10333. 1 hit.
PTHR10333:SF37. PTHR10333:SF37. 1 hit.
PfamiPF12998. ING. 1 hit.
[Graphical view]
SMARTiSM01408. ING. 1 hit.
SM00249. PHD. 1 hit.
[Graphical view]
SUPFAMiSSF57903. SSF57903. 1 hit.
PROSITEiPS01359. ZF_PHD_1. 1 hit.
PS50016. ZF_PHD_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiING2_HUMAN
AccessioniPrimary (citable) accession number: Q9H160
Secondary accession number(s): B6ZDS1, O95698
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 15, 2005
Last sequence update: February 15, 2005
Last modified: November 30, 2016
This is version 138 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 4
    Human chromosome 4: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.