ID   ATG10_HUMAN             Reviewed;         220 AA.
AC   Q9H0Y0; B2RE09; Q6PIX1; Q9H842;
DT   29-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   25-JAN-2012, entry version 81.
DE   RecName: Full=Ubiquitin-like-conjugating enzyme ATG10;
DE            EC=6.3.2.-;
DE   AltName: Full=Autophagy-related protein 10;
DE            Short=APG10-like;
GN   Name=ATG10; ORFNames=PP12616, APG10L;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC   Catarrhini; Hominidae; Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RX   PubMed=15498874; DOI=10.1073/pnas.0404089101;
RA   Wan D., Gong Y., Qin W., Zhang P., Li J., Wei L., Zhou X., Li H.,
RA   Qiu X., Zhong F., He L., Yu J., Yao G., Jiang H., Qian L., Yu Y.,
RA   Shu H., Chen X., Xu H., Guo M., Pan Z., Chen Y., Ge C., Yang S.,
RA   Gu J.;
RT   "Large-scale cDNA transfection screening for genes related to cancer
RT   development and progression.";
RL   Proc. Natl. Acad. Sci. U.S.A. 101:15724-15729(2004).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC   TISSUE=Thymus;
RX   PubMed=14702039; DOI=10.1038/ng1285;
RA   Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA   Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA   Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA   Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA   Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A.,
RA   Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M.,
RA   Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y.,
RA   Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M.,
RA   Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K.,
RA   Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S.,
RA   Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J.,
RA   Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y.,
RA   Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N.,
RA   Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S.,
RA   Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA   Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA   Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA   Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA   Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y.,
RA   Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T.,
RA   Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y.,
RA   Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S.,
RA   Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T.,
RA   Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M.,
RA   Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T.,
RA   Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K.,
RA   Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R.,
RA   Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.;
RT   "Complete sequencing and characterization of 21,243 full-length human
RT   cDNAs.";
RL   Nat. Genet. 36:40-45(2004).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC   TISSUE=Uterus;
RX   PubMed=17974005; DOI=10.1186/1471-2164-8-399;
RA   Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U.,
RA   Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H.,
RA   Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K.,
RA   Ottenwaelder B., Poustka A., Wiemann S., Schupp I.;
RT   "The full-ORF clone resource of the German cDNA consortium.";
RL   BMC Genomics 8:399-399(2007).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), AND VARIANTS
RP   MET-212 AND HIS-220.
RC   TISSUE=Placenta, and Testis;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA
RT   project: the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
CC   -!- FUNCTION: E2-like enzyme involved in autophagy. Acts as an E2-like
CC       enzyme that catalyzes the conjugation of ATG12 to ATG5. ATG12
CC       conjugation to ATG5 is required for autophagy. Likely serves as an
CC       ATG5-recognition molecule. Not involved in ATG12 conjugation to
CC       ATG3 (By similarity).
CC   -!- SUBUNIT: Interacts with MAP1LC3A. By interacting with MAP1LC3A, it
CC       plays a role in the conjugation of ATG12 to ATG5. Also able to
CC       directly interact either with ATG5 or ATG7 (By similarity).
CC   -!- INTERACTION:
CC       O94817:ATG12; NbExp=1; IntAct=EBI-1048913, EBI-746742;
CC       O95166:GABARAP; NbExp=1; IntAct=EBI-1048913, EBI-712001;
CC       Q9H0R8:GABARAPL1; NbExp=1; IntAct=EBI-1048913, EBI-746969;
CC       P60520:GABARAPL2; NbExp=1; IntAct=EBI-1048913, EBI-720116;
CC       Q9GZQ8:MAP1LC3B; NbExp=1; IntAct=EBI-1048913, EBI-373144;
CC       Q9BXW4:MAP1LC3C; NbExp=1; IntAct=EBI-1048913, EBI-2603996;
CC   -!- SUBCELLULAR LOCATION: Cytoplasm (Probable).
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=1;
CC         IsoId=Q9H0Y0-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=Q9H0Y0-2; Sequence=VSP_013272, VSP_013273;
CC   -!- SIMILARITY: Belongs to the ATG10 family.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=BC004551; Type=Frameshift; Positions=26;
CC       Sequence=BC027718; Type=Frameshift; Positions=26;
CC   -----------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution-NoDerivs License
CC   -----------------------------------------------------------------------
DR   EMBL; AF318326; AAL55833.1; -; mRNA.
DR   EMBL; AK024016; BAB14778.1; -; mRNA.
DR   EMBL; AK315752; BAG38106.1; -; mRNA.
DR   EMBL; AL136912; CAB66846.1; -; mRNA.
DR   EMBL; BC004551; -; NOT_ANNOTATED_CDS; mRNA.
DR   EMBL; BC027718; -; NOT_ANNOTATED_CDS; mRNA.
DR   EMBL; BC029268; AAH29268.1; -; mRNA.
DR   IPI; IPI00017456; -.
DR   IPI; IPI00073313; -.
DR   RefSeq; NP_001124500.1; NM_001131028.1.
DR   RefSeq; NP_113670.1; NM_031482.4.
DR   UniGene; Hs.713698; -.
DR   ProteinModelPortal; Q9H0Y0; -.
DR   IntAct; Q9H0Y0; 17.
DR   STRING; Q9H0Y0; -.
DR   DMDM; 62286633; -.
DR   PRIDE; Q9H0Y0; -.
DR   Ensembl; ENST00000282185; ENSP00000282185; ENSG00000152348.
DR   Ensembl; ENST00000458350; ENSP00000404938; ENSG00000152348.
DR   GeneID; 83734; -.
DR   KEGG; hsa:83734; -.
DR   UCSC; uc003khq.1; human.
DR   UCSC; uc003khr.1; human.
DR   CTD; 83734; -.
DR   GeneCards; GC05P081267; -.
DR   H-InvDB; HIX0022382; -.
DR   HGNC; HGNC:20315; ATG10.
DR   HPA; CAB037232; -.
DR   MIM; 610800; gene.
DR   neXtProt; NX_Q9H0Y0; -.
DR   PharmGKB; PA134872167; -.
DR   eggNOG; prNOG18412; -.
DR   GeneTree; ENSGT00390000000924; -.
DR   HOGENOM; HBG716469; -.
DR   HOVERGEN; HBG080874; -.
DR   InParanoid; Q9H0Y0; -.
DR   OMA; EWRTSKD; -.
DR   PhylomeDB; Q9H0Y0; -.
DR   NextBio; 72737; -.
DR   ArrayExpress; Q9H0Y0; -.
DR   Bgee; Q9H0Y0; -.
DR   CleanEx; HS_ATG10; -.
DR   Genevestigator; Q9H0Y0; -.
DR   GermOnline; ENSG00000152348; Homo sapiens.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0019777; F:Atg12 ligase activity; ISS:UniProtKB.
DR   GO; GO:0034263; P:autophagy in response to ER overload; IMP:UniProtKB.
DR   GO; GO:0031401; P:positive regulation of protein modification process; ISS:UniProtKB.
DR   GO; GO:0006497; P:protein lipidation; ISS:UniProtKB.
DR   GO; GO:0032446; P:protein modification by small protein conjugation; IDA:UniProtKB.
DR   GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR   InterPro; IPR007135; Autophagy-rel_prot_3.
DR   Pfam; PF03987; Autophagy_act_C; 1.
PE   1: Evidence at protein level;
KW   Alternative splicing; Autophagy; Complete proteome; Cytoplasm; Ligase;
KW   Polymorphism; Protein transport; Reference proteome; Transport;
KW   Ubl conjugation pathway.
FT   CHAIN         1    220       Ubiquitin-like-conjugating enzyme ATG10.
FT                                /FTId=PRO_0000096183.
FT   VAR_SEQ      73    125       EAFELPLDDCEVIETAAASEVIKYEYHVLYSCSYQVPVLYF
FT                                RASFLDGRPLTL -> VKSCSVTQAGVQLRDLSSLQPPPSG
FT                                FKQFSCLSLPSNWDYRGSPLHLANFLYF (in isoform
FT                                2).
FT                                /FTId=VSP_013272.
FT   VAR_SEQ     126    220       Missing (in isoform 2).
FT                                /FTId=VSP_013273.
FT   VARIANT      62     62       S -> P (in dbSNP:rs3734114).
FT                                /FTId=VAR_024370.
FT   VARIANT     212    212       T -> M (in dbSNP:rs1864183).
FT                                /FTId=VAR_021562.
FT   VARIANT     220    220       P -> H (in dbSNP:rs1864182).
FT                                /FTId=VAR_021563.
SQ   SEQUENCE   220 AA;  25279 MW;  1EDA80E385779B69 CRC64;
     MEEDEFIGEK TFQRYCAEFI KHSQQIGDSW EWRPSKDCSD GYMCKIHFQI KNGSVMSHLG
     ASTHGQTCLP MEEAFELPLD DCEVIETAAA SEVIKYEYHV LYSCSYQVPV LYFRASFLDG
     RPLTLKDIWE GVHECYKMRL LQGPWDTITQ QEHPILGQPF FVLHPCKTNE FMTPVLKNSQ
     KINKNVNYIT SWLSIVGPVV GLNLPLSYAK ATSQDERNVP
//