Q9H0Y0 (ATG10_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ubiquitin-like-conjugating enzyme ATG10
EC=6.3.2.-

Alternative name(s):
Autophagy-related protein 10
Short name=APG10-like

Gene names

Name:	ATG10
ORF Names:	APG10L, PP12616

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	220 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	E2-like enzyme involved in autophagy. Acts as an E2-like enzyme that catalyzes the conjugation of ATG12 to ATG5. ATG12 conjugation to ATG5 is required for autophagy. Likely serves as an ATG5-recognition molecule. Not involved in ATG12 conjugation to ATG3 By similarity.
Subunit structure	Interacts with MAP1LC3A. By interacting with MAP1LC3A, it plays a role in the conjugation of ATG12 to ATG5. Also able to directly interact either with ATG5 or ATG7 By similarity.
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the ATG10 family.
Sequence caution	The sequence BC004551 differs from that shown. Reason: Frameshift at position 26. The sequence BC027718 differs from that shown. Reason: Frameshift at position 26.

Ontologies

Keywords
Biological process	Autophagy Protein transport Transport Ubl conjugation pathway
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing Polymorphism
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	autophagy in response to ER overload Inferred from mutant phenotype PubMed 17256008. Source: UniProtKB positive regulation of protein modification process Inferred from sequence or structural similarity. Source: UniProtKB protein lipidation Inferred from sequence or structural similarity. Source: UniProtKB protein modification by small protein conjugation Inferred from direct assay PubMed 16963840. Source: UniProtKB protein transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell intracellular Inferred from direct assay. Source: LIFEdb
Molecular_function	Atg12 ligase activity Inferred from sequence or structural similarity PubMed 20723759. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
ATG12	O94817	1	EBI-1048913,EBI-746742
GABARAP	O95166	1	EBI-1048913,EBI-712001
GABARAPL1	Q9H0R8	1	EBI-1048913,EBI-746969
GABARAPL2	P60520	1	EBI-1048913,EBI-720116
MAP1LC3B	Q9GZQ8	1	EBI-1048913,EBI-373144
MAP1LC3C	Q9BXW4	1	EBI-1048913,EBI-2603996

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9H0Y0-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9H0Y0-2)

The sequence of this isoform differs from the canonical sequence as follows:
     73-125: EAFELPLDDC...SFLDGRPLTL → VKSCSVTQAG...PLHLANFLYF
     126-220: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 220	220	Ubiquitin-like-conjugating enzyme ATG10		PRO_0000096183
Natural variations
	Alternative sequence	73 – 125	53	EAFEL…RPLTL → VKSCSVTQAGVQLRDLSSLQ PPPSGFKQFSCLSLPSNWDY RGSPLHLANFLYF in isoform 2.		VSP_013272
	Alternative sequence	126 – 220	95	Missing in isoform 2.		VSP_013273
	Natural variant	62	1	S → P. Corresponds to variant rs3734114 [ dbSNP | Ensembl ].		VAR_024370
	Natural variant	212	1	T → M. Ref.4 Corresponds to variant rs1864183 [ dbSNP | Ensembl ].		VAR_021562
	Natural variant	220	1	P → H. Ref.4 Corresponds to variant rs1864182 [ dbSNP | Ensembl ].		VAR_021563

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: 1EDA80E385779B69	FASTA	220	25,279
	10 20 30 40 50 60 MEEDEFIGEK TFQRYCAEFI KHSQQIGDSW EWRPSKDCSD GYMCKIHFQI KNGSVMSHLG 70 80 90 100 110 120 ASTHGQTCLP MEEAFELPLD DCEVIETAAA SEVIKYEYHV LYSCSYQVPV LYFRASFLDG 130 140 150 160 170 180 RPLTLKDIWE GVHECYKMRL LQGPWDTITQ QEHPILGQPF FVLHPCKTNE FMTPVLKNSQ 190 200 210 220 KINKNVNYIT SWLSIVGPVV GLNLPLSYAK ATSQDERNVP
	Isoform 2 [UniParc]. Checksum: 57CE9269957F02CA	FASTA	125	14,217
	10 20 30 40 50 60 MEEDEFIGEK TFQRYCAEFI KHSQQIGDSW EWRPSKDCSD GYMCKIHFQI KNGSVMSHLG 70 80 90 100 110 120 ASTHGQTCLP MEVKSCSVTQ AGVQLRDLSS LQPPPSGFKQ FSCLSLPSNW DYRGSPLHLA NFLYF

References

[1]	"Large-scale cDNA transfection screening for genes related to cancer development and progression." Wan D., Gong Y., Qin W., Zhang P., Li J., Wei L., Zhou X., Li H., Qiu X., Zhong F., He L., Yu J., Yao G., Jiang H., Qian L., Yu Y., Shu H., Chen X. , Xu H., Guo M., Pan Z., Chen Y., Ge C., Yang S., Gu J. Proc. Natl. Acad. Sci. U.S.A. 101:15724-15729(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Tissue: Thymus.
[3]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Uterus.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANTS MET-212 AND HIS-220. Tissue: Placenta and Testis.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF318326 mRNA. Translation: AAL55833.1. AK024016 mRNA. Translation: BAB14778.1. AK315752 mRNA. Translation: BAG38106.1. AL136912 mRNA. Translation: CAB66846.1. BC004551 mRNA. No translation available. BC027718 mRNA. No translation available. BC029268 mRNA. Translation: AAH29268.1.
IPI	IPI00017456. IPI00073313.
RefSeq	NP_001124500.1. NM_001131028.1. NP_113670.1. NM_031482.4.
UniGene	Hs.713698.
3D structure databases
ProteinModelPortal	Q9H0Y0.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9H0Y0. 15 interactions.
STRING	9606.ENSP00000282185.
Polymorphism databases
DMDM	62286633.
Proteomic databases
PaxDb	Q9H0Y0.
PRIDE	Q9H0Y0.
Protocols and materials databases
DNASU	83734.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000282185; ENSP00000282185; ENSG00000152348. ENST00000355178; ENSP00000347309; ENSG00000152348. ENST00000458350; ENSP00000404938; ENSG00000152348. ENST00000513443; ENSP00000425182; ENSG00000152348.
GeneID	83734.
KEGG	hsa:83734.
UCSC	uc003khq.2. human. uc003khr.3. human.
Organism-specific databases
CTD	83734.
GeneCards	GC05P081267.
HGNC	HGNC:20315. ATG10.
HPA	CAB037232.
MIM	610800. gene.
neXtProt	NX_Q9H0Y0.
PharmGKB	PA134872167.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG258154.
HOGENOM	HOG000034127.
HOVERGEN	HBG080874.
InParanoid	Q9H0Y0.
OMA	TSKDCSD.
PhylomeDB	Q9H0Y0.
Gene expression databases
ArrayExpress	Q9H0Y0.
Bgee	Q9H0Y0.
CleanEx	HS_ATG10.
Genevestigator	Q9H0Y0.
GermOnline	ENSG00000152348. Homo sapiens.
Family and domain databases
InterPro	IPR007135. Autophagy-rel_prot_3. [Graphical view]
Pfam	PF03987. Autophagy_act_C. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	83734.
NextBio	72737.
SOURCE	Search...

Entry information

Entry name

ATG10_HUMAN

Accession

Primary (citable) accession number: Q9H0Y0
Secondary accession number(s): B2RE09, Q6PIX1, Q9H842

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 29, 2005
Last sequence update:	March 1, 2001
Last modified:	April 3, 2013
This is version 91 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.