Reviewed, UniProtKB/Swiss-Prot Q9H0Y0 (ATG10_HUMAN)

Names and origin

Protein names

Recommended name:
Autophagy-related protein 10

Alternative name(s):
APG10-like

Gene names

Name:	ATG10
ORF Names:	PP12616, APG10L

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	220 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Plays a role in autophagy By similarity. ATG12-conjugating enzyme (E2-like enzyme), likely serves as an ATG5-recognition molecule.
Subunit structure	Interacts with MAP1LC3A. By interacting with MAP1LC3A, it plays a role in the conjugation of ATG12 to ATG5. Also able to directly interact either with ATG5 or ATG7 By similarity.
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the ATG10 family.
Sequence caution	The sequence BC004551 differs from that shown. Reason: Frameshift at position 26. The sequence BC027718 differs from that shown. Reason: Frameshift at position 26.

Ontologies

Keywords
Biological process	Autophagy Protein transport Transport Ubl conjugation pathway
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing Polymorphism
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	autophagy in response to ER overload Inferred from mutant phenotype. Source: UniProtKB modification-dependent protein catabolic process Inferred from electronic annotation. Source: UniProtKB-KW positive regulation of protein modification process Inferred from sequence or structural similarity. Source: UniProtKB protein amino acid lipidation Inferred from sequence or structural similarity. Source: UniProtKB protein modification by small protein conjugation Inferred from direct assay. Source: UniProtKB protein transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
ATG12	O94817	1	EBI-1048913,EBI-746742

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9H0Y0-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9H0Y0-2)

The sequence of this isoform differs from the canonical sequence as follows:
     73-125: EAFELPLDDC...SFLDGRPLTL → VKSCSVTQAG...PLHLANFLYF
     126-220: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 220	220	Autophagy-related protein 10		PRO_0000096183
Natural variations
	Alternative sequence	73 – 125	53	EAFEL…RPLTL → VKSCSVTQAGVQLRDLSSLQ PPPSGFKQFSCLSLPSNWDY RGSPLHLANFLYF in isoform 2.		VSP_013272
	Alternative sequence	126 – 220	95	Missing in isoform 2.		VSP_013273
	Natural variant	62	1	S → P: dbSNP rs3734114.		VAR_024370
	Natural variant	212	1	T → M: dbSNP rs1864183. Ref.4		VAR_021562
	Natural variant	220	1	P → H: dbSNP rs1864182. Ref.4		VAR_021563

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: 1EDA80E385779B69	FASTA	220	25,279
	10 20 30 40 50 60 MEEDEFIGEK TFQRYCAEFI KHSQQIGDSW EWRPSKDCSD GYMCKIHFQI KNGSVMSHLG 70 80 90 100 110 120 ASTHGQTCLP MEEAFELPLD DCEVIETAAA SEVIKYEYHV LYSCSYQVPV LYFRASFLDG 130 140 150 160 170 180 RPLTLKDIWE GVHECYKMRL LQGPWDTITQ QEHPILGQPF FVLHPCKTNE FMTPVLKNSQ 190 200 210 220 KINKNVNYIT SWLSIVGPVV GLNLPLSYAK ATSQDERNVP
	Isoform 2. Checksum: 57CE9269957F02CA	FASTA	125	14,217
	10 20 30 40 50 60 MEEDEFIGEK TFQRYCAEFI KHSQQIGDSW EWRPSKDCSD GYMCKIHFQI KNGSVMSHLG 70 80 90 100 110 120 ASTHGQTCLP MEVKSCSVTQ AGVQLRDLSS LQPPPSGFKQ FSCLSLPSNW DYRGSPLHLA NFLYF

References

[1]	"Large-scale cDNA transfection screening for genes related to cancer development and progression." Wan D., Gong Y., Qin W., Zhang P., Li J., Wei L., Zhou X., Li H., Qiu X., Zhong F., He L., Yu J., Yao G., Jiang H., Qian L., Yu Y., Shu H., Chen X. , Xu H., Guo M., Pan Z., Chen Y., Ge C., Yang S., Gu J. Proc. Natl. Acad. Sci. U.S.A. 101:15724-15729(2004) [PubMed: 15498874] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Tissue: Thymus.
[3]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed: 17974005] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Uterus.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANTS MET-212 AND HIS-220. Tissue: Placenta and Testis.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF318326 mRNA. Translation: AAL55833.1. AK024016 mRNA. Translation: BAB14778.1. AK315752 mRNA. Translation: BAG38106.1. AL136912 mRNA. Translation: CAB66846.1. BC004551 mRNA. No translation available. BC027718 mRNA. No translation available. BC029268 mRNA. Translation: AAH29268.1.
IPI	IPI00017456. IPI00073313.
RefSeq	NP_001124500.1. NP_113670.1.
UniGene	Hs.713698
3D structure databases
SMR	Q9H0Y0. Positions 29-201.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9H0Y0. 1 interaction.
STRING	Q9H0Y0.
Genome annotation databases
Ensembl	ENST00000282185; ENSP00000282185; ENSG00000152348; Homo sapiens. [Genome view] ENST00000458350; ENSP00000404938; ENSG00000152348; Homo sapiens. [Genome view]
GeneID	83734.
KEGG	hsa:83734.
UCSC	uc003khq.1. human. uc003khr.1. human.
Organism-specific databases
CTD	83734.
GeneCards	GC05P081304.
HGNC	HGNC:20315. ATG10.
MIM	610800. gene.
PharmGKB	PA134872167.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG18412.
HOGENOM	HBG716469.
HOVERGEN	HBG080874.
InParanoid	Q9H0Y0.
OMA	ECYKTRL.
OrthoDB	EOG9SR0FZ.
PhylomeDB	Q9H0Y0.
Gene expression databases
ArrayExpress	Q9H0Y0.
Bgee	Q9H0Y0.
CleanEx	HS_ATG10.
Genevestigator	Q9H0Y0.
GermOnline	ENSG00000152348. Homo sapiens.
Family and domain databases
InterPro	IPR007135. Autophagy-rel_prot_3. [Graphical view]
Pfam	PF03987. Autophagy_act_C. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	72737.
SOURCE	Search...

Entry information

Entry name

ATG10_HUMAN

Accession

Primary (citable) accession number: Q9H0Y0
Secondary accession number(s): B2RE09, Q6PIX1, Q9H842

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 29, 2005
Last sequence update:	March 1, 2001
Last modified:	March 2, 2010
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.