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Q9H0Y0 (ATG10_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 101. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ubiquitin-like-conjugating enzyme ATG10

EC=6.3.2.-
Alternative name(s):
Autophagy-related protein 10
Short name=APG10-like
Gene names
Name:ATG10
Synonyms:APG10L
ORF Names:PP12616
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length220 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

E2-like enzyme involved in autophagy. Acts as an E2-like enzyme that catalyzes the conjugation of ATG12 to ATG5. ATG12 conjugation to ATG5 is required for autophagy. Likely serves as an ATG5-recognition molecule. Not involved in ATG12 conjugation to ATG3 By similarity. Plays a role in adenovirus-mediated cell lysis. Ref.5

Subunit structure

Interacts with MAP1LC3A. By interacting with MAP1LC3A, it plays a role in the conjugation of ATG12 to ATG5. Also able to directly interact either with ATG5 or ATG7 By similarity. Interacts with IRGM. Ref.6

Subcellular location

Cytoplasm Probable.

Sequence similarities

Belongs to the ATG10 family.

Sequence caution

The sequence BC004551 differs from that shown. Reason: Frameshift at position 26.

The sequence BC027718 differs from that shown. Reason: Frameshift at position 26.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9H0Y0-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9H0Y0-2)

The sequence of this isoform differs from the canonical sequence as follows:
     73-125: EAFELPLDDC...SFLDGRPLTL → VKSCSVTQAG...PLHLANFLYF
     126-220: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 220220Ubiquitin-like-conjugating enzyme ATG10
PRO_0000096183

Sites

Active site1661Glycyl thioester intermediate By similarity

Natural variations

Alternative sequence73 – 12553EAFEL…RPLTL → VKSCSVTQAGVQLRDLSSLQ PPPSGFKQFSCLSLPSNWDY RGSPLHLANFLYF in isoform 2.
VSP_013272
Alternative sequence126 – 22095Missing in isoform 2.
VSP_013273
Natural variant621S → P.
Corresponds to variant rs3734114 [ dbSNP | Ensembl ].
VAR_024370
Natural variant2121T → M. Ref.4
Corresponds to variant rs1864183 [ dbSNP | Ensembl ].
VAR_021562
Natural variant2201P → H. Ref.4
Corresponds to variant rs1864182 [ dbSNP | Ensembl ].
VAR_021563

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified March 1, 2001. Version 1.
Checksum: 1EDA80E385779B69

FASTA22025,279
        10         20         30         40         50         60 
MEEDEFIGEK TFQRYCAEFI KHSQQIGDSW EWRPSKDCSD GYMCKIHFQI KNGSVMSHLG 

        70         80         90        100        110        120 
ASTHGQTCLP MEEAFELPLD DCEVIETAAA SEVIKYEYHV LYSCSYQVPV LYFRASFLDG 

       130        140        150        160        170        180 
RPLTLKDIWE GVHECYKMRL LQGPWDTITQ QEHPILGQPF FVLHPCKTNE FMTPVLKNSQ 

       190        200        210        220 
KINKNVNYIT SWLSIVGPVV GLNLPLSYAK ATSQDERNVP 

« Hide

Isoform 2 [UniParc].

Checksum: 57CE9269957F02CA
Show »

FASTA12514,217

References

« Hide 'large scale' references
[1]"Large-scale cDNA transfection screening for genes related to cancer development and progression."
Wan D., Gong Y., Qin W., Zhang P., Li J., Wei L., Zhou X., Li H., Qiu X., Zhong F., He L., Yu J., Yao G., Jiang H., Qian L., Yu Y., Shu H., Chen X. expand/collapse author list , Xu H., Guo M., Pan Z., Chen Y., Ge C., Yang S., Gu J.
Proc. Natl. Acad. Sci. U.S.A. 101:15724-15729(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
[2]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Tissue: Thymus.
[3]"The full-ORF clone resource of the German cDNA consortium."
Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I.
BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Uterus.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANTS MET-212 AND HIS-220.
Tissue: Placenta and Testis.
[5]"Human adenovirus type 5 induces cell lysis through autophagy and autophagy-triggered caspase activity."
Jiang H., White E.J., Rios-Vicil C.I., Xu J., Gomez-Manzano C., Fueyo J.
J. Virol. 85:4720-4729(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[6]"IRGM is a common target of RNA viruses that subvert the autophagy network."
Gregoire I.P., Richetta C., Meyniel-Schicklin L., Borel S., Pradezynski F., Diaz O., Deloire A., Azocar O., Baguet J., Le Breton M., Mangeot P.E., Navratil V., Joubert P.E., Flacher M., Vidalain P.O., Andre P., Lotteau V., Biard-Piechaczyk M., Rabourdin-Combe C., Faure M.
PLoS Pathog. 7:E1002422-E1002422(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH IRGM.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF318326 mRNA. Translation: AAL55833.1.
AK024016 mRNA. Translation: BAB14778.1.
AK315752 mRNA. Translation: BAG38106.1.
AL136912 mRNA. Translation: CAB66846.1.
BC004551 mRNA. No translation available.
BC027718 mRNA. No translation available.
BC029268 mRNA. Translation: AAH29268.1.
RefSeqNP_001124500.1. NM_001131028.1.
NP_113670.1. NM_031482.4.
XP_005248667.1. XM_005248610.2.
XP_005248668.1. XM_005248611.2.
UniGeneHs.713698.

3D structure databases

ProteinModelPortalQ9H0Y0.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid123745. 12 interactions.
IntActQ9H0Y0. 15 interactions.
MINTMINT-4712407.
STRING9606.ENSP00000282185.

Polymorphism databases

DMDM62286633.

Proteomic databases

PaxDbQ9H0Y0.
PRIDEQ9H0Y0.

Protocols and materials databases

DNASU83734.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000282185; ENSP00000282185; ENSG00000152348. [Q9H0Y0-1]
ENST00000355178; ENSP00000347309; ENSG00000152348. [Q9H0Y0-2]
ENST00000458350; ENSP00000404938; ENSG00000152348. [Q9H0Y0-1]
ENST00000513443; ENSP00000425182; ENSG00000152348. [Q9H0Y0-2]
GeneID83734.
KEGGhsa:83734.
UCSCuc003khq.2. human. [Q9H0Y0-2]
uc003khr.3. human. [Q9H0Y0-1]

Organism-specific databases

CTD83734.
GeneCardsGC05P081267.
HGNCHGNC:20315. ATG10.
HPACAB037232.
HPA044163.
MIM610800. gene.
neXtProtNX_Q9H0Y0.
PharmGKBPA134872167.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG258154.
HOGENOMHOG000034127.
HOVERGENHBG080874.
InParanoidQ9H0Y0.
KOK17888.
OMALQQGPWD.
OrthoDBEOG7K3TN4.
PhylomeDBQ9H0Y0.
TreeFamTF314016.

Gene expression databases

ArrayExpressQ9H0Y0.
BgeeQ9H0Y0.
CleanExHS_ATG10.
GenevestigatorQ9H0Y0.

Family and domain databases

InterProIPR007135. Autophagy-rel_prot_3.
[Graphical view]
PfamPF03987. Autophagy_act_C. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiATG10.
GenomeRNAi83734.
NextBio72737.
PROQ9H0Y0.
SOURCESearch...

Entry information

Entry nameATG10_HUMAN
AccessionPrimary (citable) accession number: Q9H0Y0
Secondary accession number(s): B2RE09, Q6PIX1, Q9H842
Entry history
Integrated into UniProtKB/Swiss-Prot: March 29, 2005
Last sequence update: March 1, 2001
Last modified: April 16, 2014
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 5

Human chromosome 5: entries, gene names and cross-references to MIM