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Protein

Oxysterol-binding protein-related protein 5

Gene

OSBPL5

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Lipid transporter involved in lipid countertransport between the endoplasmic reticulum and the plasma membrane: specifically exchanges phosphatidylserine with phosphatidylinositol 4-phosphate (PI4P), delivering phosphatidylserine to the plasma membrane in exchange for PI4P, which is degraded by the SAC1/SACM1L phosphatase in the endoplasmic reticulum. Binds phosphatidylserine and PI4P in a mutually exclusive manner (PubMed:23934110, PubMed:26206935). May cooperate with NPC1 to mediate the exit of cholesterol from endosomes/lysosomes (PubMed:21220512). Binds 25-hydroxycholesterol and cholesterol (PubMed:17428193).4 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei449PhosphatidylserineBy similarity1
Binding sitei504PhosphatidylserineBy similarity1
Binding sitei670Phosphatidylinositol 4-phosphateBy similarity1
Binding sitei674Phosphatidylinositol 4-phosphateBy similarity1
Binding sitei678Phosphatidylinositol 4-phosphateBy similarity1

GO - Molecular functioni

  • cholesterol binding Source: BHF-UCL
  • oxysterol binding Source: UniProtKB
  • phosphatidylinositol-4-phosphate binding Source: UniProtKB
  • phosphatidylserine binding Source: UniProtKB
  • phospholipid transporter activity Source: UniProtKB

GO - Biological processi

  • cholesterol metabolic process Source: UniProtKB
  • cholesterol transport Source: UniProtKB
  • Golgi to plasma membrane transport Source: UniProtKB
  • phospholipid transport Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Lipid transport, Transport

Keywords - Ligandi

Lipid-binding

Enzyme and pathway databases

BioCyciZFISH:ENSG00000021762-MONOMER.
ReactomeiR-HSA-1482801. Acyl chain remodelling of PS.

Protein family/group databases

TCDBi2.D.1.1.1. the pi4p/ps counter transporter (p/p-ct) family.

Chemistry databases

SwissLipidsiSLP:000000527.

Names & Taxonomyi

Protein namesi
Recommended name:
Oxysterol-binding protein-related protein 5
Short name:
ORP-5
Short name:
OSBP-related protein 5
Alternative name(s):
Oxysterol-binding protein homolog 11 Publication
Gene namesi
Name:OSBPL5
Synonyms:KIAA1534, OBPH11 Publication, ORP5
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 11

Organism-specific databases

HGNCiHGNC:16392. OSBPL5.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei860 – 878HelicalSequence analysisAdd BLAST19

GO - Cellular componenti

  • cytosol Source: UniProtKB
  • endoplasmic reticulum membrane Source: UniProtKB-SubCell
  • integral component of membrane Source: UniProtKB-KW
  • membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi389L → D: Impaired lipid countertransport between the endoplasmic reticulum and the plasma membrane. 1 Publication1
Mutagenesisi478 – 479HH → AA: Impaired lipid countertransport between the endoplasmic reticulum and the plasma membrane. 1 Publication2

Organism-specific databases

DisGeNETi114879.
OpenTargetsiENSG00000021762.
PharmGKBiPA32829.

Polymorphism and mutation databases

BioMutaiOSBPL5.
DMDMi20139173.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001003731 – 879Oxysterol-binding protein-related protein 5Add BLAST879

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei12PhosphoserineBy similarity1
Modified residuei293PhosphoserineBy similarity1
Modified residuei301PhosphoserineBy similarity1
Modified residuei747PhosphoserineCombined sources1
Modified residuei777PhosphoserineBy similarity1
Modified residuei778PhosphoserineBy similarity1
Modified residuei780PhosphoserineBy similarity1
Modified residuei784PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9H0X9.
MaxQBiQ9H0X9.
PaxDbiQ9H0X9.
PeptideAtlasiQ9H0X9.
PRIDEiQ9H0X9.

PTM databases

iPTMnetiQ9H0X9.
PhosphoSitePlusiQ9H0X9.

Expressioni

Tissue specificityi

Ubiquitously expressed.2 Publications

Gene expression databases

BgeeiENSG00000021762.
CleanExiHS_OSBPL5.
ExpressionAtlasiQ9H0X9. baseline and differential.
GenevisibleiQ9H0X9. HS.

Organism-specific databases

HPAiHPA038712.
HPA058727.

Interactioni

Protein-protein interaction databases

BioGridi125380. 22 interactors.
DIPiDIP-52332N.
IntActiQ9H0X9. 15 interactors.
MINTiMINT-4722889.
STRINGi9606.ENSP00000263650.

Structurei

3D structure databases

ProteinModelPortaliQ9H0X9.
SMRiQ9H0X9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini126 – 243PHPROSITE-ProRule annotationAdd BLAST118

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni384 – 389Phosphatidylinositol 4-phosphate bindingBy similarity6
Regioni384 – 389Phosphatidylserine bindingBy similarity6
Regioni446 – 449Phosphatidylinositol 4-phosphate bindingBy similarity4
Regioni478 – 479Phosphatidylinositol 4-phosphate bindingBy similarity2

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili93 – 123Sequence analysisAdd BLAST31

Sequence similaritiesi

Belongs to the OSBP family.Curated
Contains 1 PH domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2210. Eukaryota.
ENOG410XRW6. LUCA.
GeneTreeiENSGT00550000074515.
HOGENOMiHOG000233870.
HOVERGENiHBG053375.
InParanoidiQ9H0X9.
KOiK20464.
OMAiFLTCQLF.
OrthoDBiEOG091G0A3R.
PhylomeDBiQ9H0X9.
TreeFamiTF312807.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
InterProiIPR000648. Oxysterol-bd.
IPR018494. Oxysterol-bd_CS.
IPR011993. PH_dom-like.
IPR001849. PH_domain.
[Graphical view]
PANTHERiPTHR10972. PTHR10972. 1 hit.
PfamiPF01237. Oxysterol_BP. 1 hit.
PF00169. PH. 1 hit.
[Graphical view]
SMARTiSM00233. PH. 1 hit.
[Graphical view]
SUPFAMiSSF50729. SSF50729. 1 hit.
PROSITEiPS01013. OSBP. 1 hit.
PS50003. PH_DOMAIN. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9H0X9-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKEEAFLRRR FSLCPPSSTP QKVDPRKLTR NLLLSGDNEL YPLSPGKDME
60 70 80 90 100
PNGPSLPRDE GPPTPSSATK VPPAEYRLCN GSDKECVSPT ARVTKKETLK
110 120 130 140 150
AQKENYRQEK KRATRQLLSA LTDPSVVIMA DSLKIRGTLK SWTKLWCVLK
160 170 180 190 200
PGVLLIYKTP KVGQWVGTVL LHCCELIERP SKKDGFCFKL FHPLDQSVWA
210 220 230 240 250
VKGPKGESVG SITQPLPSSY LIFRAASESD GRCWLDALEL ALRCSSLLRL
260 270 280 290 300
GTCKPGRDGE PGTSPDASPS SLCGLPASAT VHPDQDLFPL NGSSLENDAF
310 320 330 340 350
SDKSERENPE ESDTETQDHS RKTESGSDQS ETPGAPVRRG TTYVEQVQEE
360 370 380 390 400
LGELGEASQV ETVSEENKSL MWTLLKQLRP GMDLSRVVLP TFVLEPRSFL
410 420 430 440 450
NKLSDYYYHA DLLSRAAVEE DAYSRMKLVL RWYLSGFYKK PKGIKKPYNP
460 470 480 490 500
ILGETFRCCW FHPQTDSRTF YIAEQVSHHP PVSAFHVSNR KDGFCISGSI
510 520 530 540 550
TAKSRFYGNS LSALLDGKAT LTFLNRAEDY TLTMPYAHCK GILYGTMTLE
560 570 580 590 600
LGGKVTIECA KNNFQAQLEF KLKPFFGGST SINQISGKIT SGEEVLASLS
610 620 630 640 650
GHWDRDVFIK EEGSGSSALF WTPSGEVRRQ RLRQHTVPLE EQTELESERL
660 670 680 690 700
WQHVTRAISK GDQHRATQEK FALEEAQRQR ARERQESLMP WKPQLFHLDP
710 720 730 740 750
ITQEWHYRYE DHSPWDPLKD IAQFEQDGIL RTLQQEAVAR QTTFLGSPGP
760 770 780 790 800
RHERSGPDQR LRKASDQPSG HSQATESSGS TPESCPELSD EEQDGDFVPG
810 820 830 840 850
GESPCPRCRK EARRLQALHE AILSIREAQQ ELHRHLSAML SSTARAAQAP
860 870
TPGLLQSPRS WFLLCVFLAC QLFINHILK
Length:879
Mass (Da):98,616
Last modified:March 1, 2001 - v1
Checksum:i7EF06544347CC60A
GO
Isoform 2 (identifier: Q9H0X9-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     134-201: Missing.

Note: No experimental confirmation available.
Show »
Length:811
Mass (Da):90,794
Checksum:iECBEEE16DCF1B998
GO
Isoform 3 (identifier: Q9H0X9-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-48: Missing.
     161-201: Missing.

Note: No experimental confirmation available.
Show »
Length:790
Mass (Da):88,435
Checksum:i6DAA25EEFBC0CEB6
GO

Sequence cautioni

The sequence BAA96058 differs from that shown. Contaminating sequence. Sequence of unknown origin in the N-terminal part.Curated

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_06007990T → I.Corresponds to variant rs6578323dbSNPEnsembl.1
Natural variantiVAR_020414774A → T.Corresponds to variant rs2277301dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0574081 – 48Missing in isoform 3. 1 PublicationAdd BLAST48
Alternative sequenceiVSP_043071134 – 201Missing in isoform 2. 1 PublicationAdd BLAST68
Alternative sequenceiVSP_057409161 – 201Missing in isoform 3. 1 PublicationAdd BLAST41

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB074006 mRNA. Translation: BAB85686.1.
AF392453 mRNA. Translation: AAL40666.1.
AF410430 mRNA. Translation: AAK98617.1.
AL136918 mRNA. Translation: CAB66852.1.
AK056510 mRNA. Translation: BAG51735.1.
AK301003 mRNA. Translation: BAG62622.1.
AC108448 Genomic DNA. No translation available.
KC877382 Genomic DNA. No translation available.
KF455307 Genomic DNA. No translation available.
KF455310 Genomic DNA. No translation available.
CH471158 Genomic DNA. Translation: EAX02542.1.
CH471158 Genomic DNA. Translation: EAX02543.1.
BC032646 mRNA. Translation: AAH32646.2.
BC039579 mRNA. Translation: AAH39579.1.
AF331964 mRNA. Translation: AAG53417.1.
AB040967 mRNA. Translation: BAA96058.1. Sequence problems.
CCDSiCCDS31343.1. [Q9H0X9-2]
CCDS31344.1. [Q9H0X9-1]
RefSeqiNP_001137535.1. NM_001144063.1. [Q9H0X9-2]
NP_065947.1. NM_020896.3. [Q9H0X9-1]
NP_663613.1. NM_145638.2. [Q9H0X9-2]
XP_011518175.1. XM_011519873.2. [Q9H0X9-1]
XP_016872652.1. XM_017017163.1. [Q9H0X9-1]
UniGeneiHs.436166.

Genome annotation databases

EnsembliENST00000263650; ENSP00000263650; ENSG00000021762. [Q9H0X9-1]
ENST00000348039; ENSP00000302872; ENSG00000021762. [Q9H0X9-2]
ENST00000389989; ENSP00000374639; ENSG00000021762. [Q9H0X9-2]
ENST00000525498; ENSP00000433342; ENSG00000021762. [Q9H0X9-3]
GeneIDi114879.
KEGGihsa:114879.
UCSCiuc001lxk.3. human. [Q9H0X9-1]
uc010qxq.2. human.

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB074006 mRNA. Translation: BAB85686.1.
AF392453 mRNA. Translation: AAL40666.1.
AF410430 mRNA. Translation: AAK98617.1.
AL136918 mRNA. Translation: CAB66852.1.
AK056510 mRNA. Translation: BAG51735.1.
AK301003 mRNA. Translation: BAG62622.1.
AC108448 Genomic DNA. No translation available.
KC877382 Genomic DNA. No translation available.
KF455307 Genomic DNA. No translation available.
KF455310 Genomic DNA. No translation available.
CH471158 Genomic DNA. Translation: EAX02542.1.
CH471158 Genomic DNA. Translation: EAX02543.1.
BC032646 mRNA. Translation: AAH32646.2.
BC039579 mRNA. Translation: AAH39579.1.
AF331964 mRNA. Translation: AAG53417.1.
AB040967 mRNA. Translation: BAA96058.1. Sequence problems.
CCDSiCCDS31343.1. [Q9H0X9-2]
CCDS31344.1. [Q9H0X9-1]
RefSeqiNP_001137535.1. NM_001144063.1. [Q9H0X9-2]
NP_065947.1. NM_020896.3. [Q9H0X9-1]
NP_663613.1. NM_145638.2. [Q9H0X9-2]
XP_011518175.1. XM_011519873.2. [Q9H0X9-1]
XP_016872652.1. XM_017017163.1. [Q9H0X9-1]
UniGeneiHs.436166.

3D structure databases

ProteinModelPortaliQ9H0X9.
SMRiQ9H0X9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi125380. 22 interactors.
DIPiDIP-52332N.
IntActiQ9H0X9. 15 interactors.
MINTiMINT-4722889.
STRINGi9606.ENSP00000263650.

Chemistry databases

SwissLipidsiSLP:000000527.

Protein family/group databases

TCDBi2.D.1.1.1. the pi4p/ps counter transporter (p/p-ct) family.

PTM databases

iPTMnetiQ9H0X9.
PhosphoSitePlusiQ9H0X9.

Polymorphism and mutation databases

BioMutaiOSBPL5.
DMDMi20139173.

Proteomic databases

EPDiQ9H0X9.
MaxQBiQ9H0X9.
PaxDbiQ9H0X9.
PeptideAtlasiQ9H0X9.
PRIDEiQ9H0X9.

Protocols and materials databases

DNASUi114879.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000263650; ENSP00000263650; ENSG00000021762. [Q9H0X9-1]
ENST00000348039; ENSP00000302872; ENSG00000021762. [Q9H0X9-2]
ENST00000389989; ENSP00000374639; ENSG00000021762. [Q9H0X9-2]
ENST00000525498; ENSP00000433342; ENSG00000021762. [Q9H0X9-3]
GeneIDi114879.
KEGGihsa:114879.
UCSCiuc001lxk.3. human. [Q9H0X9-1]
uc010qxq.2. human.

Organism-specific databases

CTDi114879.
DisGeNETi114879.
GeneCardsiOSBPL5.
HGNCiHGNC:16392. OSBPL5.
HPAiHPA038712.
HPA058727.
MIMi606733. gene.
neXtProtiNX_Q9H0X9.
OpenTargetsiENSG00000021762.
PharmGKBiPA32829.
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2210. Eukaryota.
ENOG410XRW6. LUCA.
GeneTreeiENSGT00550000074515.
HOGENOMiHOG000233870.
HOVERGENiHBG053375.
InParanoidiQ9H0X9.
KOiK20464.
OMAiFLTCQLF.
OrthoDBiEOG091G0A3R.
PhylomeDBiQ9H0X9.
TreeFamiTF312807.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000021762-MONOMER.
ReactomeiR-HSA-1482801. Acyl chain remodelling of PS.

Miscellaneous databases

ChiTaRSiOSBPL5. human.
GeneWikiiOSBPL5.
GenomeRNAii114879.
PROiQ9H0X9.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000021762.
CleanExiHS_OSBPL5.
ExpressionAtlasiQ9H0X9. baseline and differential.
GenevisibleiQ9H0X9. HS.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
InterProiIPR000648. Oxysterol-bd.
IPR018494. Oxysterol-bd_CS.
IPR011993. PH_dom-like.
IPR001849. PH_domain.
[Graphical view]
PANTHERiPTHR10972. PTHR10972. 1 hit.
PfamiPF01237. Oxysterol_BP. 1 hit.
PF00169. PH. 1 hit.
[Graphical view]
SMARTiSM00233. PH. 1 hit.
[Graphical view]
SUPFAMiSSF50729. SSF50729. 1 hit.
PROSITEiPS01013. OSBP. 1 hit.
PS50003. PH_DOMAIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiOSBL5_HUMAN
AccessioniPrimary (citable) accession number: Q9H0X9
Secondary accession number(s): A6NDP0
, A6NJS8, B4DVB0, Q54A90, Q8N596, Q9BZB0, Q9P1Z4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 11, 2002
Last sequence update: March 1, 2001
Last modified: November 30, 2016
This is version 136 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Miscellaneous

According to a report, the gene is imprinted in placenta, where it is predominantly expressed from the maternal allele only. Not imprinted in other tissues (PubMed:12504849). According to another report, it is not imprinted in trophoblast stem cells (PubMed:20644730).2 Publications

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.