Q9H0X4 (ITFG3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein ITFG3

Gene names

Name:	ITFG3
Synonyms:	C16orf9

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	552 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Subcellular location	Membrane; Single-pass type II membrane protein Potential.
Sequence similarities	Belongs to the ITFG3 family.
Sequence caution	The sequence AAK61220.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Cellular component	Membrane
Coding sequence diversity	Alternative splicing
Domain	Signal-anchor Transmembrane Transmembrane helix
PTM	Glycoprotein Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9H0X4-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9H0X4-2)

The sequence of this isoform differs from the canonical sequence as follows:
     516-540: VRDLVPSSRVVRLGEGGPDSDQAIR → AHTGRQRRGSWTGQSSRRDLHVRVL
     541-552: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 552	552	Protein ITFG3		PRO_0000247993
Regions
	Topological domain	1 – 49	49	Cytoplasmic Potential
	Transmembrane	50 – 70	21	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	71 – 552	482	Extracellular Potential
Amino acid modifications
	Modified residue	21	1	Phosphoserine Ref.8
	Glycosylation	116	1	N-linked (GlcNAc...) Potential
	Glycosylation	314	1	N-linked (GlcNAc...) Ref.7
	Glycosylation	389	1	N-linked (GlcNAc...) Ref.6 Ref.7
	Glycosylation	473	1	N-linked (GlcNAc...) Ref.7
Natural variations
	Alternative sequence	516 – 540	25	VRDLV…DQAIR → AHTGRQRRGSWTGQSSRRDL HVRVL in isoform 2.		VSP_020121
	Alternative sequence	541 – 552	12	Missing in isoform 2.		VSP_020122
Experimental info
	Sequence conflict	20	1	K → E in BAB71374. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: AC81CB184EC3B043	FASTA	552	59,660
	10 20 30 40 50 60 MLDHKDLEAE IHPLKNEERK SQENLGNPSK NEDNVKSAPP QSRLSRCRAA AFFLSLFLCL 70 80 90 100 110 120 FVVFVVSFVI PCPDRPASQR MWRIDYSAAV IYDFLAVDDI NGDRIQDVLF LYKNTNSSNN 130 140 150 160 170 180 FSRSCVDEGF SSPCTFAAAV SGANGSTLWE RPVAQDVALV ECAVPQPRGS EAPSACILVG 190 200 210 220 230 240 RPSSFIAVNL FTGETLWNHS SSFSGNASIL SPLLQVPDVD GDGAPDLLVL TQEREEVSGH 250 260 270 280 290 300 LYSGSTGHQI GLRGSLGVDG ESGFLLHVTR TGAHYILFPC ASSLCGCSVK GLYEKVTGSG 310 320 330 340 350 360 GPFKSDPHWE SMLNATTRRM LSHSSGAVRY LMHVPGNAGA DVLLVGSEAF VLLDGQELTP 370 380 390 400 410 420 RWTPKAAHVL RKPIFGRYKP DTLAVAVENG TGTDRQILFL DLGTGAVLCS LALPSLPGGP 430 440 450 460 470 480 LSASLPTADH RSAFFFWGLH ELGSTSETET GEARHSLYMF HPTLPRVLLE LANVSTHIVA 490 500 510 520 530 540 FDAVLFEPSR HAAYILLTGP ADSEAPGLVS VIKHKVRDLV PSSRVVRLGE GGPDSDQAIR 550 DRFSRLRYQS EA
	Isoform 2 [UniParc]. Checksum: 4031F2348BA6637E	FASTA	540	58,388
	10 20 30 40 50 60 MLDHKDLEAE IHPLKNEERK SQENLGNPSK NEDNVKSAPP QSRLSRCRAA AFFLSLFLCL 70 80 90 100 110 120 FVVFVVSFVI PCPDRPASQR MWRIDYSAAV IYDFLAVDDI NGDRIQDVLF LYKNTNSSNN 130 140 150 160 170 180 FSRSCVDEGF SSPCTFAAAV SGANGSTLWE RPVAQDVALV ECAVPQPRGS EAPSACILVG 190 200 210 220 230 240 RPSSFIAVNL FTGETLWNHS SSFSGNASIL SPLLQVPDVD GDGAPDLLVL TQEREEVSGH 250 260 270 280 290 300 LYSGSTGHQI GLRGSLGVDG ESGFLLHVTR TGAHYILFPC ASSLCGCSVK GLYEKVTGSG 310 320 330 340 350 360 GPFKSDPHWE SMLNATTRRM LSHSSGAVRY LMHVPGNAGA DVLLVGSEAF VLLDGQELTP 370 380 390 400 410 420 RWTPKAAHVL RKPIFGRYKP DTLAVAVENG TGTDRQILFL DLGTGAVLCS LALPSLPGGP 430 440 450 460 470 480 LSASLPTADH RSAFFFWGLH ELGSTSETET GEARHSLYMF HPTLPRVLLE LANVSTHIVA 490 500 510 520 530 540 FDAVLFEPSR HAAYILLTGP ADSEAPGLVS VIKHKAHTGR QRRGSWTGQS SRRDLHVRVL

References

[1]	"Towards a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs." Wiemann S., Weil B., Wellenreuther R., Gassenhuber J., Glassl S., Ansorge W., Boecher M., Bloecker H., Bauersachs S., Blum H., Lauber J., Duesterhoeft A., Beyer A., Koehrer K., Strack N., Mewes H.-W., Ottenwaelder B., Obermaier B. , Tampe J., Heubner D., Wambutt R., Korn B., Klein M., Poustka A. Genome Res. 11:422-435(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Amygdala.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Stomach.
[3]	"Sequence, structure and pathology of the fully annotated terminal 2 Mb of the short arm of human chromosome 16." Daniels R.J., Peden J.F., Lloyd C., Horsley S.W., Clark K., Tufarelli C., Kearney L., Buckle V.J., Doggett N.A., Flint J., Higgs D.R. Hum. Mol. Genet. 10:339-352(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Colon, Glioblastoma and Ovary.
[6]	"Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry." Chen R., Jiang X., Sun D., Han G., Wang F., Ye M., Wang L., Zou H. J. Proteome Res. 8:651-661(2009) [PubMed] [Europe PMC] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-389, MASS SPECTROMETRY. Tissue: Liver.
[7]	"Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins." Wollscheid B., Bausch-Fluck D., Henderson C., O'Brien R., Bibel M., Schiess R., Aebersold R., Watts J.D. Nat. Biotechnol. 27:378-386(2009) [PubMed] [Europe PMC] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-314; ASN-389 AND ASN-473, MASS SPECTROMETRY. Tissue: Leukemic T-cell.
[8]	"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation." Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B. Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-21, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL136542 mRNA. Translation: CAB66477.1. AK057165 mRNA. Translation: BAB71374.1. AE006462 Genomic DNA. Translation: AAK61220.1. Sequence problems. CH471112 Genomic DNA. Translation: EAW85846.1. CH471112 Genomic DNA. Translation: EAW85847.1. BC010521 mRNA. Translation: AAH10521.1. BC013047 mRNA. Translation: AAH13047.1. BC032112 mRNA. Translation: AAH32112.1.
IPI	IPI00396658. IPI00845516.
RefSeq	NP_114428.1. NM_032039.2.
UniGene	Hs.513225.
3D structure databases
ProteinModelPortal	Q9H0X4.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9H0X4. 1 interaction.
STRING	9606.ENSP00000301678.
PTM databases
PhosphoSite	Q9H0X4.
Polymorphism databases
DMDM	74733531.
Proteomic databases
PaxDb	Q9H0X4.
PRIDE	Q9H0X4.
Protocols and materials databases
DNASU	83986.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000301678; ENSP00000301678; ENSG00000167930. ENST00000301679; ENSP00000301679; ENSG00000167930. ENST00000399932; ENSP00000382814; ENSG00000167930. ENST00000450082; ENSP00000411394; ENSG00000167930.
GeneID	83986.
KEGG	hsa:83986.
UCSC	uc002cgf.3. human. uc002cgg.2. human.
Organism-specific databases
CTD	83986.
GeneCards	GC16P000289.
HGNC	HGNC:14163. ITFG3.
HPA	HPA011747.
neXtProt	NX_Q9H0X4.
PharmGKB	PA25564.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG79285.
HOGENOM	HOG000069926.
HOVERGEN	HBG056541.
InParanoid	Q9H0X4.
OMA	YMFHPTL.
OrthoDB	EOG4GXFMJ.
PhylomeDB	Q9H0X4.
Gene expression databases
ArrayExpress	Q9H0X4.
Bgee	Q9H0X4.
CleanEx	HS_ITFG3.
Genevestigator	Q9H0X4.
GermOnline	ENSG00000167930. Homo sapiens.
Family and domain databases
InterPro	IPR011047. Quinonprotein_ADH-like_supfam. [Graphical view]
SUPFAM	SSF50998. Quin_alc_DH_like. 1 hit.
ProtoNet	Search...
Other
GenomeRNAi	83986.
NextBio	73118.

Entry information

Entry name

ITFG3_HUMAN

Accession

Primary (citable) accession number: Q9H0X4
Secondary accession number(s): D3DU45 , Q7L416, Q96FR1, Q96MC7, Q96S30

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 5, 2006
Last sequence update:	March 1, 2001
Last modified:	May 1, 2013
This is version 90 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.