Q9H0W9 (CK054_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ester hydrolase C11orf54
EC=3.1.-.-

Gene names

Name:	C11orf54
ORF Names:	LP4947, PTD012

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	315 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Exhibits ester hydrolase activity on the substrate p-nitrophenyl acetate. Ref.9
Subunit structure	Monomer. Ref.9
Subcellular location	Nucleus Ref.9.
Sequence caution	The sequence AAH07110.2 differs from that shown. Reason: Erroneous initiation. The sequence AAP34483.1 differs from that shown. Reason: Frameshift at position 125.

Ontologies

Keywords
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Ligand	Metal-binding Zinc
Molecular function	Hydrolase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	nucleus Inferred from direct assay. Source: LIFEdb
Molecular_function	hydrolase activity, acting on ester bonds Inferred from direct assay Ref.9. Source: FlyBase zinc ion binding Inferred from direct assay Ref.9. Source: FlyBase
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
TRIP13	Q15645	3	EBI-740204,EBI-358993

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9H0W9-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9H0W9-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-19: Missing.

Isoform 3 (identifier: Q9H0W9-3)

The sequence of this isoform differs from the canonical sequence as follows:
     170-219: Missing.

Note: Probably non-functional.

Isoform 4 (identifier: Q9H0W9-4)

The sequence of this isoform differs from the canonical sequence as follows:
     1-111: Missing.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 315

315

Ester hydrolase C11orf54

PRO_0000246029

Sites

Metal binding

266

1

Zinc; catalytic

Metal binding

268

1

Zinc; catalytic

Metal binding

278

1

Zinc; catalytic

Natural variations

Alternative sequence

1 – 111

111

Missing in isoform 4.

VSP_019817

Alternative sequence

1 – 19

19

Missing in isoform 2.

VSP_019818

Alternative sequence

170 – 219

50

Missing in isoform 3.

VSP_019821

Experimental info

Sequence conflict

10

1

V → A in CAG38569. Ref.3

Sequence conflict

184

1

F → L in CAG38569. Ref.3

Sequence conflict

304

1

D → G in AAD40375. Ref.1

Secondary structure

1

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315

Helix Strand Turn

Details...

Beta strand

4 – 7

4

Helix

13 – 27

15

Beta strand

28 – 37

10

Turn

44 – 46

3

Beta strand

49 – 51

3

Beta strand

54 – 56

3

Beta strand

58 – 63

6

Helix

65 – 67

3

Beta strand

69 – 71

3

Beta strand

77 – 79

3

Helix

80 – 86

7

Beta strand

93 – 99

7

Helix

102 – 105

4

Beta strand

110 – 112

3

Beta strand

114 – 116

3

Beta strand

129 – 133

5

Turn

135 – 137

3

Beta strand

140 – 144

5

Helix

145 – 148

4

Beta strand

153 – 163

11

Beta strand

169 – 178

10

Helix

184 – 196

13

Beta strand

202 – 218

17

Helix

231 – 237

7

Beta strand

238 – 245

8

Beta strand

247 – 256

10

Beta strand

264 – 270

7

Beta strand

272 – 274

3

Beta strand

277 – 283

7

Turn

285 – 287

3

Beta strand

289 – 296

8

Beta strand

298 – 304

7

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: F084671CC1E3F72A	FASTA	315	35,117
	10 20 30 40 50 60 MACAEFSFHV PSLEELAGVM QKGLKDNFAD VQVSVVDCPD LTKEPFTFPV KGICGKTRIA 70 80 90 100 110 120 EVGGVPYLLP LVNQKKVYDL NKIAKEIKLP GAFILGAGAG PFQTLGFNSE FMPVIQTESE 130 140 150 160 170 180 HKPPVNGSYF AHVNPADGGC LLEKYSEKCH DFQCALLANL FASEGQPGKV IEVKAKRRTG 190 200 210 220 230 240 PLNFVTCMRE TLEKHYGNKP IGMGGTFIIQ KGKVKSHIMP AEFSSCPLNS DEEVNKWLHF 250 260 270 280 290 300 YEMKAPLVCL PVFVSRDPGF DLRLEHTHFF SRHGEGGHYH YDTTPDIVEY LGYFLPAEFL 310 YRIDQPKETH SIGRD
	Isoform 2 [UniParc]. Checksum: 66962958EE2A4F17	FASTA	296	33,098
	10 20 30 40 50 60 MQKGLKDNFA DVQVSVVDCP DLTKEPFTFP VKGICGKTRI AEVGGVPYLL PLVNQKKVYD 70 80 90 100 110 120 LNKIAKEIKL PGAFILGAGA GPFQTLGFNS EFMPVIQTES EHKPPVNGSY FAHVNPADGG 130 140 150 160 170 180 CLLEKYSEKC HDFQCALLAN LFASEGQPGK VIEVKAKRRT GPLNFVTCMR ETLEKHYGNK 190 200 210 220 230 240 PIGMGGTFII QKGKVKSHIM PAEFSSCPLN SDEEVNKWLH FYEMKAPLVC LPVFVSRDPG 250 260 270 280 290 FDLRLEHTHF FSRHGEGGHY HYDTTPDIVE YLGYFLPAEF LYRIDQPKET HSIGRD
	Isoform 3 [UniParc]. Checksum: 82F671A9F47F0906	FASTA	265	29,492
	10 20 30 40 50 60 MACAEFSFHV PSLEELAGVM QKGLKDNFAD VQVSVVDCPD LTKEPFTFPV KGICGKTRIA 70 80 90 100 110 120 EVGGVPYLLP LVNQKKVYDL NKIAKEIKLP GAFILGAGAG PFQTLGFNSE FMPVIQTESE 130 140 150 160 170 180 HKPPVNGSYF AHVNPADGGC LLEKYSEKCH DFQCALLANL FASEGQPGKP AEFSSCPLNS 190 200 210 220 230 240 DEEVNKWLHF YEMKAPLVCL PVFVSRDPGF DLRLEHTHFF SRHGEGGHYH YDTTPDIVEY 250 260 LGYFLPAEFL YRIDQPKETH SIGRD
	Isoform 4 [UniParc]. Checksum: DBE4CAF56964ADFD	FASTA	204	23,173
	10 20 30 40 50 60 MPVIQTESEH KPPVNGSYFA HVNPADGGCL LEKYSEKCHD FQCALLANLF ASEGQPGKVI 70 80 90 100 110 120 EVKAKRRTGP LNFVTCMRET LEKHYGNKPI GMGGTFIIQK GKVKSHIMPA EFSSCPLNSD 130 140 150 160 170 180 EEVNKWLHFY EMKAPLVCLP VFVSRDPGFD LRLEHTHFFS RHGEGGHYHY DTTPDIVEYL 190 200 GYFLPAEFLY RIDQPKETHS IGRD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Human PTD012 mRNA, complete cds." Mao Y., Song H., Peng Y., Huang Q., Dai M., Zhang Q., Mao M., Fu G., Luo M., Chen J., Hu R. Submitted (SEP-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 4). Tissue: Pituitary tumor.
[2]	"Towards a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs." Wiemann S., Weil B., Wellenreuther R., Gassenhuber J., Glassl S., Ansorge W., Boecher M., Bloecker H., Bauersachs S., Blum H., Lauber J., Duesterhoeft A., Beyer A., Koehrer K., Strack N., Mewes H.-W., Ottenwaelder B., Obermaier B. , Tampe J., Heubner D., Wambutt R., Korn B., Klein M., Poustka A. Genome Res. 11:422-435(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Brain.
[3]	"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[4]	"Large-scale cDNA transfection screening for genes related to cancer development and progression." Wan D., Gong Y., Qin W., Zhang P., Li J., Wei L., Zhou X., Li H., Qiu X., Zhong F., He L., Yu J., Yao G., Jiang H., Qian L., Yu Y., Shu H., Chen X. , Xu H., Guo M., Pan Z., Chen Y., Ge C., Yang S., Gu J. Proc. Natl. Acad. Sci. U.S.A. 101:15724-15729(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[5]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Esophagus.
[6]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3). Tissue: Colon and Skeletal muscle.
[8]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[9]	"Crystal structure of Homo sapiens PTD012 reveals a zinc-containing hydrolase fold." Manjasetty B.A., Buessow K., Fieber-Erdmann M., Roske Y., Gobom J., Scheich C., Goetz F., Niesen F.H., Heinemann U. Protein Sci. 15:914-920(2006) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS) OF 2-315, FUNCTION, SUBUNIT, SUBCELLULAR LOCATION, ALTERNATIVE SPLICING.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF092133 mRNA. Translation: AAD40375.1.
AL136605 mRNA. Translation: CAB66540.1.
CR533538 mRNA. Translation: CAG38569.1.
AY203960 mRNA. Translation: AAP34483.1. Frameshift.
AK292215 mRNA. Translation: BAF84904.1.
CH471065 Genomic DNA. Translation: EAW66915.1.
BC007110 mRNA. Translation: AAH07110.2. Different initiation.
BC012298 mRNA. Translation: AAH12298.1.

IPI

IPI00061507.
IPI00072044.
IPI00760666.
IPI00761072.

RefSeq

NP_054758.2. NM_014039.2.

UniGene

Hs.8360.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1XCR	X-ray	1.70	A/B	2-315	[»]

ProteinModelPortal

Q9H0W9.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q9H0W9. 1 interaction.

MINT

MINT-1455099.

STRING

9606.ENSP00000331209.

PTM databases

PhosphoSite

Q9H0W9.

Polymorphism databases

DMDM

74718025.

Proteomic databases

PaxDb

Q9H0W9.

PRIDE

Q9H0W9.

Protocols and materials databases

DNASU

28970.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000331239; ENSP00000331209; ENSG00000182919.
ENST00000354421; ENSP00000346403; ENSG00000182919.
ENST00000524485; ENSP00000435107; ENSG00000182919.
ENST00000528099; ENSP00000435113; ENSG00000182919.
ENST00000528288; ENSP00000433721; ENSG00000182919.
ENST00000540113; ENSP00000442094; ENSG00000182919.

GeneID

28970.

KEGG

hsa:28970.

UCSC

uc001pef.3. human.
uc001peg.3. human.

Organism-specific databases

CTD

28970.

GeneCards

GC11P093474.

H-InvDB

HIX0010026.

HGNC

HGNC:30204. C11orf54.

HPA

HPA040088.
HPA040511.

neXtProt

NX_Q9H0W9.

PharmGKB

PA143485349.

GenAtlas

Search...

Phylogenomic databases

eggNOG

NOG330250.

HOVERGEN

HBG081215.

InParanoid

Q9H0W9.

OMA

HIMPAEF.

PhylomeDB

Q9H0W9.

Gene expression databases

ArrayExpress

Q9H0W9.

Bgee

Q9H0W9.

CleanEx

HS_C11orf54.

Genevestigator

Q9H0W9.

GermOnline

ENSG00000182919. Homo sapiens.

Family and domain databases

InterPro

IPR015021. DUF1907.
[Graphical view]

Pfam

PF08925. DUF1907. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

Q9H0W9.

GenomeRNAi

28970.

NextBio

51831.

Entry information

Entry name

CK054_HUMAN

Accession

Primary (citable) accession number: Q9H0W9
Secondary accession number(s): A8K850 , Q6FI88, Q6XYB0, Q96EI3, Q96IX1, Q9Y6B4

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 11, 2006
Last sequence update:	March 1, 2001
Last modified:	May 1, 2013
This is version 84 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.