Q9H0W8 (SMG9_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein SMG9

Alternative name(s):
Protein smg-9 homolog

Gene names

Name:	SMG9
Synonyms:	C19orf61

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	520 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in nonsense-mediated decay (NMD) of mRNAs containing premature stop codons. Is recruited by release factors to stalled ribosomes together with SMG1 and SMG8 (forming the SMG1C protein kinase complex) and, in the SMG1C complex, is required for the efficient association between SMG1 and SMG8. Ref.7
Subunit structure	Component of the SMG1C complex composed of SMG1, SMG8 and SMG9. Self-associates to form homodimers and forms heterodimers with SMG8; these assembly forms may represent SMG1C intermediate forms. Ref.7 Ref.12
Post-translational modification	Phosphorylated by SMG1. Ref.7
Sequence similarities	Belongs to the SMG9 family.
Sequence caution	The sequence AAC17932.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Nonsense-mediated mRNA decay
Coding sequence diversity	Alternative splicing
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	gene expression Traceable author statement. Source: Reactome nuclear-transcribed mRNA catabolic process, nonsense-mediated decay Inferred from mutant phenotype Ref.7. Source: UniProtKB
Cellular_component	cytosol Traceable author statement. Source: Reactome
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
itself		3	EBI-2872322,EBI-2872322
SMG8	Q8ND04	4	EBI-2872322,EBI-3903643

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9H0W8-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9H0W8-2)

The sequence of this isoform differs from the canonical sequence as follows:
     496-520: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.9
	Chain	2 – 520	519	Protein SMG9		PRO_0000289163
Regions
	Compositional bias	71 – 133	63	Pro-rich
Amino acid modifications
	Modified residue	2	1	N-acetylserine Ref.9 Ref.13
	Modified residue	7	1	Phosphoserine Ref.5 Ref.9 Ref.13
	Modified residue	32	1	Phosphoserine Ref.6 Ref.9 Ref.13
	Modified residue	53	1	Phosphoserine Ref.6 Ref.8
	Modified residue	451	1	Phosphoserine Ref.6
Natural variations
	Alternative sequence	496 – 520	25	Missing in isoform 2.		VSP_025946

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: AA68A473F7F6BBCC	FASTA	520	57,651
	10 20 30 40 50 60 MSESGHSQPG LYGIERRRRW KEPGSGGPQN LSGPGGRERD YIAPWERERR DASEETSTSV 70 80 90 100 110 120 MQKTPIILSK PPAERSKQPP PPTAPAAPPA PAPLEKPIVL MKPREEGKGP VAVTGASTPE 130 140 150 160 170 180 GTAPPPPAAP APPKGEKEGQ RPTQPVYQIQ NRGMGTAAPA AMDPVVGQAK LLPPERMKHS 190 200 210 220 230 240 IKLVDDQMNW CDSAIEYLLD QTDVLVVGVL GLQGTGKSMV MSLLSANTPE EDQRTYVFRA 250 260 270 280 290 300 QSAEMKERGG NQTSGIDFFI TQERIVFLDT QPILSPSILD HLINNDRKLP PEYNLPHTYV 310 320 330 340 350 360 EMQSLQIAAF LFTVCHVVIV VQDWFTDLSL YRFLQTAEMV KPSTPSPSHE SSSSSGSDEG 370 380 390 400 410 420 TEYYPHLVFL QNKARREDFC PRKLRQMHLM IDQLMAHSHL RYKGTLSMLQ CNVFPGLPPD 430 440 450 460 470 480 FLDSEVNLFL VPFMDSEAES ENPPRAGPGS SPLFSLLPGY RGHPSFQSLV SKLRSQVMSM 490 500 510 520 ARPQLSHTIL TEKNWFHYAA RIWDGVRKSS ALAEYSRLLA
	Isoform 2 [UniParc]. Checksum: 7831F793135ECF1D	FASTA	495	54,787
	10 20 30 40 50 60 MSESGHSQPG LYGIERRRRW KEPGSGGPQN LSGPGGRERD YIAPWERERR DASEETSTSV 70 80 90 100 110 120 MQKTPIILSK PPAERSKQPP PPTAPAAPPA PAPLEKPIVL MKPREEGKGP VAVTGASTPE 130 140 150 160 170 180 GTAPPPPAAP APPKGEKEGQ RPTQPVYQIQ NRGMGTAAPA AMDPVVGQAK LLPPERMKHS 190 200 210 220 230 240 IKLVDDQMNW CDSAIEYLLD QTDVLVVGVL GLQGTGKSMV MSLLSANTPE EDQRTYVFRA 250 260 270 280 290 300 QSAEMKERGG NQTSGIDFFI TQERIVFLDT QPILSPSILD HLINNDRKLP PEYNLPHTYV 310 320 330 340 350 360 EMQSLQIAAF LFTVCHVVIV VQDWFTDLSL YRFLQTAEMV KPSTPSPSHE SSSSSGSDEG 370 380 390 400 410 420 TEYYPHLVFL QNKARREDFC PRKLRQMHLM IDQLMAHSHL RYKGTLSMLQ CNVFPGLPPD 430 440 450 460 470 480 FLDSEVNLFL VPFMDSEAES ENPPRAGPGS SPLFSLLPGY RGHPSFQSLV SKLRSQVMSM 490 ARPQLSHTIL TEKNW

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Towards a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs." Wiemann S., Weil B., Wellenreuther R., Gassenhuber J., Glassl S., Ansorge W., Boecher M., Bloecker H., Bauersachs S., Blum H., Lauber J., Duesterhoeft A., Beyer A., Koehrer K., Strack N., Mewes H.-W., Ottenwaelder B., Obermaier B. , Tampe J., Heubner D., Wambutt R., Korn B., Klein M., Poustka A. Genome Res. 11:422-435(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Brain.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Teratocarcinoma.
[3]	"The DNA sequence and biology of human chromosome 19." Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V. , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M. Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Muscle.
[5]	"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle." Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M. Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-7, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[6]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-32; SER-53 AND SER-451, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[7]	"SMG-8 and SMG-9, two novel subunits of the SMG-1 complex, regulate remodeling of the mRNA surveillance complex during nonsense-mediated mRNA decay." Yamashita A., Izumi N., Kashima I., Ohnishi T., Saari B., Katsuhata Y., Muramatsu R., Morita T., Iwamatsu A., Hachiya T., Kurata R., Hirano H., Anderson P., Ohno S. Genes Dev. 23:1091-1105(2009) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, PHOSPHORYLATION, IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE SMG1C COMPLEX.
[8]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-53, MASS SPECTROMETRY. Tissue: Leukemic T-cell.
[9]	"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis." Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M. Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-7 AND SER-32, MASS SPECTROMETRY, CLEAVAGE OF INITIATOR METHIONINE. Tissue: Cervix carcinoma.
[10]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[11]	"The nonsense-mediated mRNA decay SMG-1 kinase is regulated by large-scale conformational changes controlled by SMG-8." Arias-Palomo E., Yamashita A., Fernandez I.S., Nunez-Ramirez R., Bamba Y., Izumi N., Ohno S., Llorca O. Genes Dev. 25:153-164(2011) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH SMG1 AND SMG8, ELECTRON MICROSCOPY OF THE SMG1C COMPLEX.
[12]	"Characterization of SMG-9, an essential component of the nonsense-mediated mRNA decay SMG1C complex." Fernandez I.S., Yamashita A., Arias-Palomo E., Bamba Y., Bartolome R.A., Canales M.A., Teixido J., Ohno S., Llorca O. Nucleic Acids Res. 39:347-358(2011) [PubMed] [Europe PMC] [Abstract] Cited for: SUBUNIT.
[13]	"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation." Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B. Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-7 AND SER-32, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL136606 mRNA. Translation: CAB66541.1. AK022948 mRNA. Translation: BAB14323.1. AC004780 Genomic DNA. Translation: AAC17932.1. Sequence problems. BC008869 mRNA. Translation: AAH08869.1.
IPI	IPI00012669. IPI00847553.
RefSeq	NP_061981.2. NM_019108.2.
UniGene	Hs.466875.
3D structure databases
ProteinModelPortal	Q9H0W8.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9H0W8. 5 interactions.
STRING	9606.ENSP00000270066.
PTM databases
PhosphoSite	Q9H0W8.
Polymorphism databases
DMDM	74733529.
Proteomic databases
PaxDb	Q9H0W8.
PRIDE	Q9H0W8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000270066; ENSP00000270066; ENSG00000105771. ENST00000601170; ENSP00000471398; ENSG00000105771.
GeneID	56006.
KEGG	hsa:56006.
UCSC	uc002oxj.2. human.
Organism-specific databases
CTD	56006.
GeneCards	GC19M044236.
HGNC	HGNC:25763. SMG9.
HPA	HPA041763. HPA042096.
MIM	613176. gene.
neXtProt	NX_Q9H0W8.
PharmGKB	PA162378734.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG255645.
HOGENOM	HOG000006915.
OMA	HSHLKYK.
OrthoDB	EOG4P5K91.
Enzyme and pathway databases
Reactome	REACT_21257. Metabolism of RNA. REACT_71. Gene Expression.
Gene expression databases
Bgee	Q9H0W8.
CleanEx	HS_C19orf61.
Genevestigator	Q9H0W8.
Family and domain databases
InterPro	IPR019354. Smg8/Smg9. [Graphical view]
Pfam	PF10220. DUF2146. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	56006.
NextBio	61462.
SOURCE	Search...

Entry information

Entry name

SMG9_HUMAN

Accession

Primary (citable) accession number: Q9H0W8
Secondary accession number(s): O60429, Q9H9A9

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 29, 2007
Last sequence update:	March 1, 2001
Last modified:	May 1, 2013
This is version 83 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.