Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Probable ATP-dependent RNA helicase DDX47

Gene

DDX47

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in apoptosis. May have a role in rRNA processing and mRNA splicing. Associates with pre-rRNA precursors.2 Publications

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi68 – 758ATP

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • ATP-dependent RNA helicase activity Source: GO_Central
  • poly(A) RNA binding Source: UniProtKB

GO - Biological processi

  • extrinsic apoptotic signaling pathway via death domain receptors Source: UniProtKB
  • mRNA processing Source: UniProtKB-KW
  • RNA secondary structure unwinding Source: GO_Central
  • RNA splicing Source: UniProtKB
  • rRNA processing Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Biological processi

Apoptosis, mRNA processing, mRNA splicing, rRNA processing

Keywords - Ligandi

ATP-binding, Nucleotide-binding, RNA-binding

Enzyme and pathway databases

ReactomeiR-HSA-6790901. rRNA modification in the nucleus.
R-HSA-6791226. Major pathway of rRNA processing in the nucleolus.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable ATP-dependent RNA helicase DDX47 (EC:3.6.4.13)
Alternative name(s):
DEAD box protein 47
Gene namesi
Name:DDX47
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 12

Organism-specific databases

HGNCiHGNC:18682. DDX47.

Subcellular locationi

GO - Cellular componenti

  • membrane Source: UniProtKB
  • nucleolus Source: UniProtKB
  • nucleoplasm Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134918403.

Polymorphism and mutation databases

BioMutaiDDX47.
DMDMi52782792.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedCombined sources1 Publication
Chaini2 – 455454Probable ATP-dependent RNA helicase DDX47PRO_0000055050Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineCombined sources1 Publication
Modified residuei9 – 91PhosphoserineCombined sources
Modified residuei149 – 1491PhosphothreonineCombined sources
Modified residuei424 – 4241PhosphoserineCombined sources

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9H0S4.
MaxQBiQ9H0S4.
PaxDbiQ9H0S4.
PeptideAtlasiQ9H0S4.
PRIDEiQ9H0S4.

2D gel databases

SWISS-2DPAGEQ9H0S4.

PTM databases

iPTMnetiQ9H0S4.
PhosphoSiteiQ9H0S4.
SwissPalmiQ9H0S4.

Expressioni

Gene expression databases

BgeeiENSG00000213782.
CleanExiHS_DDX47.
ExpressionAtlasiQ9H0S4. baseline and differential.
GenevisibleiQ9H0S4. HS.

Organism-specific databases

HPAiHPA014855.

Interactioni

Subunit structurei

Interacts with AGO1 and AGO2. Interacts with GABARAP. Interacts with NOL8; the interaction is RNA-dependent.4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
GABARAPO951663EBI-2515241,EBI-712001

Protein-protein interaction databases

BioGridi119375. 55 interactions.
IntActiQ9H0S4. 33 interactions.
MINTiMINT-3056694.
STRINGi9606.ENSP00000350698.

Structurei

Secondary structure

1
455
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi18 – 236Combined sources
Helixi27 – 293Combined sources
Helixi33 – 419Combined sources
Helixi49 – 5911Combined sources
Beta strandi64 – 674Combined sources
Helixi74 – 8815Combined sources
Beta strandi95 – 984Combined sources
Helixi102 – 11615Combined sources
Helixi117 – 1193Combined sources
Beta strandi123 – 1264Combined sources
Helixi132 – 1409Combined sources
Beta strandi144 – 1485Combined sources
Helixi150 – 15910Combined sources
Beta strandi170 – 1734Combined sources
Helixi176 – 1816Combined sources
Helixi185 – 1939Combined sources
Beta strandi197 – 20711Combined sources
Helixi210 – 21910Combined sources
Beta strandi224 – 2274Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3BERX-ray1.40A5-230[»]
ProteinModelPortaliQ9H0S4.
SMRiQ9H0S4. Positions 11-440.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9H0S4.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini55 – 226172Helicase ATP-bindingPROSITE-ProRule annotationAdd
BLAST
Domaini237 – 397161Helicase C-terminalPROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi24 – 5229Q motifAdd
BLAST
Motifi174 – 1774DEAD box

Sequence similaritiesi

Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0330. Eukaryota.
ENOG410XQU7. LUCA.
GeneTreeiENSGT00730000110914.
HOGENOMiHOG000268802.
HOVERGENiHBG100512.
InParanoidiQ9H0S4.
KOiK14777.
OMAiIFCGTCN.
OrthoDBiEOG091G07QH.
PhylomeDBiQ9H0S4.
TreeFamiTF105714.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR011545. DEAD/DEAH_box_helicase_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR000629. RNA-helicase_DEAD-box_CS.
IPR014014. RNA_helicase_DEAD_Q_motif.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF00271. Helicase_C. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00039. DEAD_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS51195. Q_MOTIF. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9H0S4-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAAPEEHDSP TEASQPIVEE EETKTFKDLG VTDVLCEACD QLGWTKPTKI
60 70 80 90 100
QIEAIPLALQ GRDIIGLAET GSGKTGAFAL PILNALLETP QRLFALVLTP
110 120 130 140 150
TRELAFQISE QFEALGSSIG VQSAVIVGGI DSMSQSLALA KKPHIIIATP
160 170 180 190 200
GRLIDHLENT KGFNLRALKY LVMDEADRIL NMDFETEVDK ILKVIPRDRK
210 220 230 240 250
TFLFSATMTK KVQKLQRAAL KNPVKCAVSS KYQTVEKLQQ YYIFIPSKFK
260 270 280 290 300
DTYLVYILNE LAGNSFMIFC STCNNTQRTA LLLRNLGFTA IPLHGQMSQS
310 320 330 340 350
KRLGSLNKFK AKARSILLAT DVASRGLDIP HVDVVVNFDI PTHSKDYIHR
360 370 380 390 400
VGRTARAGRS GKAITFVTQY DVELFQRIEH LIGKKLPGFP TQDDEVMMLT
410 420 430 440 450
ERVAEAQRFA RMELREHGEK KKRSREDAGD NDDTEGAIGV RNKVAGGKMK

KRKGR
Length:455
Mass (Da):50,647
Last modified:March 1, 2001 - v1
Checksum:i5F64F26AD2C11286
GO
Isoform 2 (identifier: Q9H0S4-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     251-299: Missing.

Note: No experimental confirmation available.
Show »
Length:406
Mass (Da):45,169
Checksum:iE6FFADA03AFAB679
GO

Sequence cautioni

The sequence AAF23354 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti55 – 551I → V in BAB70762 (PubMed:16541075).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei251 – 29949Missing in isoform 2. 1 PublicationVSP_045239Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL136666 mRNA. Translation: CAB66601.1.
AK054574 mRNA. Translation: BAB70762.1.
AK127712 mRNA. Translation: BAG54556.1.
AC007215 Genomic DNA. No translation available.
CH471094 Genomic DNA. Translation: EAW96277.1.
CH471094 Genomic DNA. Translation: EAW96283.1.
BC009379 mRNA. Translation: AAH09379.2.
BC068009 mRNA. Translation: AAH68009.1.
AF078843 mRNA. Translation: AAF23354.1. Different initiation.
CCDSiCCDS8655.1. [Q9H0S4-1]
CCDS8656.1. [Q9H0S4-2]
RefSeqiNP_057439.2. NM_016355.3. [Q9H0S4-1]
NP_957518.1. NM_201224.1. [Q9H0S4-2]
UniGeneiHs.719938.

Genome annotation databases

EnsembliENST00000352940; ENSP00000319578; ENSG00000213782. [Q9H0S4-2]
ENST00000358007; ENSP00000350698; ENSG00000213782. [Q9H0S4-1]
GeneIDi51202.
KEGGihsa:51202.
UCSCiuc001rax.4. human. [Q9H0S4-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL136666 mRNA. Translation: CAB66601.1.
AK054574 mRNA. Translation: BAB70762.1.
AK127712 mRNA. Translation: BAG54556.1.
AC007215 Genomic DNA. No translation available.
CH471094 Genomic DNA. Translation: EAW96277.1.
CH471094 Genomic DNA. Translation: EAW96283.1.
BC009379 mRNA. Translation: AAH09379.2.
BC068009 mRNA. Translation: AAH68009.1.
AF078843 mRNA. Translation: AAF23354.1. Different initiation.
CCDSiCCDS8655.1. [Q9H0S4-1]
CCDS8656.1. [Q9H0S4-2]
RefSeqiNP_057439.2. NM_016355.3. [Q9H0S4-1]
NP_957518.1. NM_201224.1. [Q9H0S4-2]
UniGeneiHs.719938.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3BERX-ray1.40A5-230[»]
ProteinModelPortaliQ9H0S4.
SMRiQ9H0S4. Positions 11-440.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi119375. 55 interactions.
IntActiQ9H0S4. 33 interactions.
MINTiMINT-3056694.
STRINGi9606.ENSP00000350698.

PTM databases

iPTMnetiQ9H0S4.
PhosphoSiteiQ9H0S4.
SwissPalmiQ9H0S4.

Polymorphism and mutation databases

BioMutaiDDX47.
DMDMi52782792.

2D gel databases

SWISS-2DPAGEQ9H0S4.

Proteomic databases

EPDiQ9H0S4.
MaxQBiQ9H0S4.
PaxDbiQ9H0S4.
PeptideAtlasiQ9H0S4.
PRIDEiQ9H0S4.

Protocols and materials databases

DNASUi51202.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000352940; ENSP00000319578; ENSG00000213782. [Q9H0S4-2]
ENST00000358007; ENSP00000350698; ENSG00000213782. [Q9H0S4-1]
GeneIDi51202.
KEGGihsa:51202.
UCSCiuc001rax.4. human. [Q9H0S4-1]

Organism-specific databases

CTDi51202.
GeneCardsiDDX47.
HGNCiHGNC:18682. DDX47.
HPAiHPA014855.
MIMi615428. gene.
neXtProtiNX_Q9H0S4.
PharmGKBiPA134918403.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0330. Eukaryota.
ENOG410XQU7. LUCA.
GeneTreeiENSGT00730000110914.
HOGENOMiHOG000268802.
HOVERGENiHBG100512.
InParanoidiQ9H0S4.
KOiK14777.
OMAiIFCGTCN.
OrthoDBiEOG091G07QH.
PhylomeDBiQ9H0S4.
TreeFamiTF105714.

Enzyme and pathway databases

ReactomeiR-HSA-6790901. rRNA modification in the nucleus.
R-HSA-6791226. Major pathway of rRNA processing in the nucleolus.

Miscellaneous databases

ChiTaRSiDDX47. human.
EvolutionaryTraceiQ9H0S4.
GeneWikiiDDX47.
GenomeRNAii51202.
PROiQ9H0S4.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000213782.
CleanExiHS_DDX47.
ExpressionAtlasiQ9H0S4. baseline and differential.
GenevisibleiQ9H0S4. HS.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR011545. DEAD/DEAH_box_helicase_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR000629. RNA-helicase_DEAD-box_CS.
IPR014014. RNA_helicase_DEAD_Q_motif.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF00271. Helicase_C. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00039. DEAD_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS51195. Q_MOTIF. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDDX47_HUMAN
AccessioniPrimary (citable) accession number: Q9H0S4
Secondary accession number(s): B3KXP4
, G5E955, Q96GM0, Q96NV8, Q9UI98
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 27, 2004
Last sequence update: March 1, 2001
Last modified: September 7, 2016
This is version 144 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 12
    Human chromosome 12: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.