Reviewed, UniProtKB/Swiss-Prot Q9H0R8 (GBRL1_HUMAN)

Names and origin

Protein names

Recommended name:
    Gamma-aminobutyric acid receptor-associated protein-like 1
Alternative name(s):
    GABA(A) receptor-associated protein-like 1
    Glandular epithelial cell protein 1
      Short name=GEC-1
    Early estrogen-regulated protein

Gene names

Name:	GABARAPL1
Synonyms:	GEC1

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	117 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Increases cell-surface expression of kappa-type opioid receptor through facilitating anterograde intracellular trafficking of the receptor. Ref.7
Subunit structure	Interacts with GABRG2 and beta-tubulin By similarity. Interacts with OPRK1.
Subcellular location	Cytoplasm. Membrane By similarity. Endoplasmic reticulum By similarity. Golgi apparatus By similarity.
Tissue specificity	Ubiquitous. Expressed at very high levels in the brain, heart, peripheral blood leukocytes, liver, kidney, placenta and skeletal muscle. Expressed at very low levels in thymus and small intestine. Ref.1 Ref.2
Sequence similarities	Belongs to the MAP1 LC3 family.

Ontologies

Keywords
Cellular component	Cytoplasm Endoplasmic reticulum Golgi apparatus Membrane Microtubule
Technical term	3D-structure
Gene Ontology (GO)
Cellular component	Golgi apparatus Inferred from electronic annotation. Source: UniProtKB-SubCell autophagic vacuole Inferred from direct assay. Source: MGI endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-SubCell membrane Inferred from electronic annotation. Source: UniProtKB-SubCell microtubule Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	GABA receptor binding Inferred from sequence or structural similarity. Source: UniProtKB beta-tubulin binding Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
NSF	P18708	1	EBI-746969,EBI-925742	From a different organism.
OPRK1	P41145	5	EBI-746969,EBI-925028

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 117

117

Gamma-aminobutyric acid receptor-associated protein-like 1

PRO_0000212369

Regions

Region

1 – 22

22

Interaction with beta-tubulin By similarity

Region

36 – 68

33

Interaction with GABRG2 By similarity

Secondary structure

1

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117

Helix Strand Turn

Details...

Helix

4 – 8

5

Helix

11 – 24

14

Beta strand

28 – 35

8

Beta strand

48 – 52

5

Helix

57 – 67

11

Beta strand

77 – 80

4

Helix

91 – 98

8

Beta strand

105 – 110

6

Sequences

	Sequence		Length	Mass (Da)
	Q9H0R8-1 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: 7F56E345255F564B	FASTA	117	14,044
	10 20 30 40 50 60 MKFQYKEDHP FEYRKKEGEK IRKKYPDRVP VIVEKAPKAR VPDLDKRKYL VPSDLTVGQF 70 80 90 100 110 YFLIRKRIHL RPEDALFFFV NNTIPPTSAT MGQLYEDNHE EDYFLYVAYS DESVYGK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A novel early estrogen-regulated gene gec1 encodes a protein related to GABARAP." Vernier-Magnin S., Muller S., Sallot M., Radom J., Musard J.-F., Adami P., Dulieu P., Remy-Martin J.-P., Jouvenot M., Fraichard A. Biochem. Biophys. Res. Commun. 284:118-125(2001) [PubMed: 11374880] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY. Tissue: Placenta.
[2]	"Cloning, expression patterns, and chromosome localization of three human and two mouse homologues of GABA(A) receptor-associated protein." Xin Y., Yu L., Chen Z., Zheng L., Fu Q., Jiang J., Zhang P., Gong R., Zhao S. Genomics 74:408-413(2001) [PubMed: 11414770] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
[3]	"Towards a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs." Wiemann S., Weil B., Wellenreuther R., Gassenhuber J., Glassl S., Ansorge W., Boecher M., Bloecker H., Bauersachs S., Blum H., Lauber J., Duesterhoeft A., Beyer A., Koehrer K., Strack N., Mewes H.-W., Ottenwaelder B., Obermaier B. , Tampe J., Heubner D., Wambutt R., Korn B., Klein M., Poustka A. Genome Res. 11:422-435(2001) [PubMed: 11230166] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[4]	"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain and Colon.
[7]	"GEC1 interacts with the kappa opioid receptor and enhances expression of the receptor." Chen C., Li J.-G., Chen Y., Huang P., Wang Y., Liu-Chen L.-Y. J. Biol. Chem. 281:7983-7993(2006) [PubMed: 16431922] [Abstract] Cited for: FUNCTION, INTERACTION WITH OPRK1.
[8]	Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
[9]	"Crystal structure of human gamma-aminobutyric acid receptor-associated protein-like 1 (GABARAP1)." Structural genomics consortium (SGC) Submitted (SEP-2007) to the PDB data bank Cited for: X-RAY CRYSTALLOGRAPHY (1.65 ANGSTROMS) OF 3-111.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

AF287012 mRNA. Translation: AAK55962.1.
AF087847 mRNA. Translation: AAK20399.1.
AL136676 mRNA. Translation: CAB66611.1.
CR533480 mRNA. Translation: CAG38511.1.
CH471094 Genomic DNA. Translation: EAW96164.1.
BC009309 mRNA. Translation: AAH09309.1.
BC028315 mRNA. Translation: AAH28315.1.

IPI

IPI00027733.

RefSeq

NP_113600.1.

UniGene

Hs.524250

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2R2Q	X-ray	1.65	A/B	3-111	[»]

ModBase

Search...

Protein-protein interaction databases

IntAct

Q9H0R8. 5 interactions.

PTM databases

PhosphoSite

Q9H0R8.

Proteomic databases

PRIDE

Q9H0R8.

Genome annotation databases

Ensembl

ENSG00000139112. Homo sapiens. [Contig view]

GeneID

23710.

KEGG

hsa:23710.

Organism-specific databases

GeneCards

GC12P010256.

H-InvDB

HIX0010424.
HIX0036758.

HGNC

HGNC:4068. GABARAPL1.

MIM

607420. gene.

PharmGKB

PA28481.

GenAtlas

Search...

Phylogenomic databases

HOGENOM

Q9H0R8.

HOVERGEN

Q9H0R8.

OMA

Q9H0R8. FIFVNDT.

Gene expression databases

ArrayExpress

Q9H0R8.

Bgee

Q9H0R8.

CleanEx

HS_GABARAPL1.

GermOnline

ENSG00000139112. Homo sapiens.

Family and domain databases

InterPro

IPR004241. MAP1_LC3.
[Graphical view]

PANTHER

PTHR10969. MAP1_LC3. 1 hit.

Pfam

PF02991. MAP1_LC3. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

46611.

PMAP-CutDB

Q9H0R8.

SOURCE

Search...

Entry information

Entry name

GBRL1_HUMAN

Accession

Primary (citable) accession number: Q9H0R8
Secondary accession number(s): Q6FIE6

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 2004
Last sequence update:	March 1, 2001
Last modified:	June 16, 2009
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)