Q9H0R6 (GATA_HUMAN) Reviewed, UniProtKB/Swiss-Prot
Last modified
January 25, 2012.
Version 74.
History...
Names·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Glutamyl-tRNA(Gln) amidotransferase subunit A, mitochondrial Short name=Glu-AdT subunit A EC=6.3.5.- Alternative name(s): Glutaminyl-tRNA synthase-like protein 1 | ||
| Gene names |
| ||
| Organism | Homo sapiens (Human) | ||
| Taxonomic identifier | 9606 [NCBI] | ||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Protein attributes
| Sequence length | 528 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at protein level |
General annotation (Comments)
| Function | Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in the mitochondria. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln). Ref.6 |
| Catalytic activity | ATP + L-glutamyl-tRNA(Gln) + L-glutamine = ADP + phosphate + L-glutaminyl-tRNA(Gln) + L-glutamate. |
| Subunit structure | Subunit of the heterotrimeric GatCAB amidotransferase (AdT) complex, composed of A (QRSL1), B (PET112) and C (GATC) subunits. |
| Subcellular location | |
| Sequence similarities | Belongs to the amidase family. GatA subfamily. |
| Sequence caution | The sequence CAH72094.1 differs from that shown. Reason: Erroneous gene model prediction. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Protein biosynthesis |
| Cellular component | Mitochondrion |
| Coding sequence diversity | Alternative splicing Polymorphism |
| Ligand | ATP-binding Nucleotide-binding |
| Molecular function | Ligase |
| Technical term | Complete proteome Reference proteome |
| Gene Ontology (GO) | |
| Biological process | glutaminyl-tRNAGln biosynthesis via transamidation Inferred from direct assay Ref.6. Source: UniProtKB mitochondrial translationInferred from mutant phenotype Ref.6. Source: UniProtKB |
| Cellular component | glutamyl-tRNA(Gln) amidotransferase complex Inferred from direct assay Ref.6. Source: UniProtKB mitochondrionInferred from direct assay Ref.6. Source: UniProtKB |
| Molecular function | ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutaminyl-tRNA synthase (glutamine-hydrolyzing) activityInferred from direct assay Ref.6. Source: UniProtKB |
| Complete GO annotation... | |
Alternative products
| This entry describes 2 isoforms produced by alternative splicing. [Align] [Select] | ||||||
| Isoform 1 (identifier: Q9H0R6-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. | ||||||
| Isoform 2 (identifier: Q9H0R6-2) The sequence of this isoform differs from the canonical sequence as follows: 284-304: EYLVPELSSEVQSLWSKAADL → VTFSFHYFTEILSSPIESTD 305-528: Missing. |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 528 | 528 | Glutamyl-tRNA(Gln) amidotransferase subunit A, mitochondrial | PRO_0000316767 | |||||
Sites | |||||||||
| Active site | 76 | 1 | Charge relay system By similarity | ||||||
| Active site | 171 | 1 | Charge relay system By similarity | ||||||
| Active site | 195 | 1 | Acyl-ester intermediate By similarity | ||||||
Natural variations | |||||||||
| Alternative sequence | 284 – 304 | 21 | EYLVP…KAADL → VTFSFHYFTEILSSPIESTD in isoform 2. | VSP_030773 | |||||
| Alternative sequence | 305 – 528 | 224 | Missing in isoform 2. | VSP_030774 | |||||
| Natural variant | 11 | 1 | A → V. Corresponds to variant rs36016898 [ dbSNP | Ensembl ]. | VAR_038389 | |||||
| Natural variant | 263 | 1 | N → S. Corresponds to variant rs34221917 [ dbSNP | Ensembl ]. | VAR_038390 | |||||
Experimental info | |||||||||
| Sequence conflict | 34 | 1 | T → A in CAB66614. Ref.1 | ||||||
| Sequence conflict | 147 | 1 | Q → R in CAB66614. Ref.1 | ||||||
| Sequence conflict | 172 | 1 | S → P in CAB66614. Ref.1 | ||||||
Sequences
| ||||||||||||||||||||||||
References
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | AL136679 mRNA. Translation: CAB66614.1. AK001851 mRNA. Translation: BAA91941.1. AK022251 mRNA. Translation: BAB13996.1. AK023509 mRNA. Translation: BAB14592.1. AL390074 Genomic DNA. Translation: CAH72093.1. AL390074 Genomic DNA. Translation: CAH72094.1. Sequence problems. CH471051 Genomic DNA. Translation: EAW48407.1. BC006084 mRNA. Translation: AAH06084.1. BC014389 mRNA. Translation: AAH14389.1. |
| IPI | IPI00090720. IPI00386916. |
| RefSeq | NP_060762.3. NM_018292.4. |
| UniGene | Hs.406917. |
3D structure databases | |
| HSSP | HSSP built from PDB template 2GI3 based on UniProtKB Q9X0Z9. |
| ProteinModelPortal | Q9H0R6. |
| SMR | Q9H0R6. Positions 1-505. |
| ModBase | Search... |
Protein-protein interaction databases | |
| DIP | DIP-48970N. |
| IntAct | Q9H0R6. 1 interaction. |
| STRING | Q9H0R6. |
Polymorphism databases | |
| DMDM | 167016573. |
Proteomic databases | |
| PRIDE | Q9H0R6. |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| Ensembl | ENST00000369046; ENSP00000358042; ENSG00000130348. |
| GeneID | 55278. |
| KEGG | hsa:55278. |
| UCSC | uc003prl.1. human. uc003prm.1. human. |
Organism-specific databases | |
| CTD | 55278. |
| GeneCards | GC06P107077. |
| HGNC | HGNC:21020. QRSL1. |
| HPA | HPA029585. HPA029587. |
| neXtProt | NX_Q9H0R6. |
| PharmGKB | PA128394680. |
| GenAtlas | Search... |
Phylogenomic databases | |
| eggNOG | prNOG14324. |
| GeneTree | ENSGT00550000074866. |
| HOGENOM | HBG481888. |
| HOVERGEN | HBG101134. |
| InParanoid | Q9H0R6. |
| OMA | TESSCYG. |
| OrthoDB | EOG4NZTT8. |
| PhylomeDB | Q9H0R6. |
Gene expression databases | |
| ArrayExpress | Q9H0R6. |
| Bgee | Q9H0R6. |
| CleanEx | HS_QRSL1. |
| Genevestigator | Q9H0R6. |
Family and domain databases | |
| InterPro | IPR000120. Amidase. IPR023631. Amidase_dom. IPR004412. GatA. [Graphical view] |
| Gene3D | G3DSA:3.90.1300.10. Amidase_sig_enz. 1 hit. |
| PANTHER | PTHR11895. Amidase. 1 hit. |
| Pfam | PF01425. Amidase. 1 hit. [Graphical view] |
| SUPFAM | SSF75304. Amidase_sig_enz. 1 hit. |
| TIGRFAMs | TIGR00132. GatA. 1 hit. |
| PROSITE | PS00571. AMIDASES. False negative. [Graphical view] |
| ProtoNet | Search... |
Other | |
| NextBio | 59416. |
Entry information
| Entry name | GATA_HUMAN | ||||||||
| Accession | Primary (citable) accession number: Q9H0R6 Secondary accession number(s): Q5VWJ4, Q9HA60, Q9NV19 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Chordata Protein Annotation Program | ||||||||
| Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. | ||||||||
Relevant documents
| Human chromosome 6 Human chromosome 6: entries, gene names and cross-references to MIM |
| Human entries with polymorphisms or disease mutations List of human entries with polymorphisms or disease mutations |
| Human polymorphisms and disease mutations Index of human polymorphisms and disease mutations |
| SIMILARITY comments Index of protein domains and families |

Clusters with