Q9H0L4 (CSTFT_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cleavage stimulation factor subunit 2 tau variant

Alternative name(s):
CF-1 64 kDa subunit tau variant
Cleavage stimulation factor 64 kDa subunit tau variant
Short name=CSTF 64 kDa subunit tau variant
TauCstF-64

Gene names

Name:	CSTF2T
Synonyms:	KIAA0689

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	616 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May play a significant role in AAUAAA-independent mRNA polyadenylation in germ cells. Directly involved in the binding to pre-mRNAs By similarity.
Subcellular location	Nucleus By similarity.
Sequence similarities	Contains 1 RRM (RNA recognition motif) domain.

Ontologies

Keywords
Biological process	mRNA processing
Cellular component	Nucleus
Domain	Repeat
Ligand	RNA-binding
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	mRNA processing Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	intracellular Inferred from direct assay. Source: LIFEdb nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	RNA binding Inferred from electronic annotation. Source: UniProtKB-KW nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 616	616	Cleavage stimulation factor subunit 2 tau variant		PRO_0000081534
Regions
	Domain	16 – 94	79	RRM
	Repeat	418 – 422	5	1-1
	Repeat	423 – 427	5	1-2; approximate
	Repeat	428 – 432	5	1-3; approximate
	Repeat	433 – 437	5	1-4; approximate
	Repeat	438 – 442	5	1-5; approximate
	Repeat	443 – 447	5	1-6
	Repeat	448 – 452	5	1-7; approximate
	Repeat	453 – 457	5	1-8; approximate
	Repeat	458 – 462	5	1-9; approximate
	Repeat	505 – 509	5	2-1
	Repeat	510 – 514	5	2-2
	Repeat	515 – 519	5	2-3
	Repeat	520 – 524	5	2-4
	Repeat	525 – 529	5	2-5; approximate
	Repeat	530 – 534	5	2-6
	Repeat	535 – 539	5	2-7; approximate
	Repeat	540 – 544	5	2-8; approximate
	Repeat	545 – 549	5	2-9; approximate
	Region	418 – 462	45	9 X 5 AA tandem repeats of M-E-T-R-[AG]
	Region	505 – 549	45	9 X 5 AA tandem repeats of G-[AT]-G-[MI]-Q
	Compositional bias	260 – 556	297	Gly-rich
Experimental info
	Sequence conflict	22	1	N → S in BAD96294. Ref.4
	Sequence conflict	553	1	K → R in AAH28239. Ref.6

Sequences

	Sequence		Length	Mass (Da)
	Q9H0L4 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: 916BB9B0802AC847	FASTA	616	64,437
	10 20 30 40 50 60 MSSLAVRDPA MDRSLRSVFV GNIPYEATEE QLKDIFSEVG SVVSFRLVYD RETGKPKGYG 70 80 90 100 110 120 FCEYQDQETA LSAMRNLNGR EFSGRALRVD NAASEKNKEE LKSLGPAAPI IDSPYGDPID 130 140 150 160 170 180 PEDAPESITR AVASLPPEQM FELMKQMKLC VQNSHQEARN MLLQNPQLAY ALLQAQVVMR 190 200 210 220 230 240 IMDPEIALKI LHRKIHVTPL IPGKSQSVSV SGPGPGPGPG LCPGPNVLLN QQNPPAPQPQ 250 260 270 280 290 300 HLARRPVKDI PPLMQTPIQG GIPAPGPIPA AVPGAGPGSL TPGGAMQPQL GMPGVGPVPL 310 320 330 340 350 360 ERGQVQMSDP RAPIPRGPVT PGGLPPRGLL GDAPNDPRGG TLLSVTGEVE PRGYLGPPHQ 370 380 390 400 410 420 GPPMHHASGH DTRGPSSHEM RGGPLGDPRL LIGEPRGPMI DQRGLPMDGR GGRDSRAMET 430 440 450 460 470 480 RAMETEVLET RVMERRGMET CAMETRGMEA RGMDARGLEM RGPVPSSRGP MTGGIQGPGP 490 500 510 520 530 540 INIGAGGPPQ GPRQVPGISG VGNPGAGMQG TGIQGTGMQG AGIQGGGMQG AGIQGVSIQG 550 560 570 580 590 600 GGIQGGGIQG ASKQGGSQPS SFSPGQSQVT PQDQEKAALI MQVLQLTADQ IAMLPPEQRQ 610 SILILKEQIQ KSTGAS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The gene CSTF2T, encoding the human variant CstF-64 polyadenylation protein CstF-64, lacks introns and may be associated with male sterility." Dass B., McDaniel L., Schultz R.A., Attaya E., MacDonald C.C. Genomics 80:509-514(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Testis.
[2]	"Towards a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs." Wiemann S., Weil B., Wellenreuther R., Gassenhuber J., Glassl S., Ansorge W., Boecher M., Bloecker H., Bauersachs S., Blum H., Lauber J., Duesterhoeft A., Beyer A., Koehrer K., Strack N., Mewes H.-W., Ottenwaelder B., Obermaier B. , Tampe J., Heubner D., Wambutt R., Korn B., Klein M., Poustka A. Genome Res. 11:422-435(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Testis.
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Placenta.
[4]	Suzuki Y., Sugano S., Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S. Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Coronary artery.
[5]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[7]	"Prediction of the coding sequences of unidentified human genes. X. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro." Ishikawa K., Nagase T., Suyama M., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O. DNA Res. 5:169-176(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 70-616. Tissue: Brain.
[8]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Leukemic T-cell.
[9]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[10]	"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation." Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B. Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY130299 mRNA. Translation: AAN05429.1. AL136747 mRNA. Translation: CAB66681.1. AK222574 mRNA. Translation: BAD96294.1. AK314318 mRNA. Translation: BAG36966.1. CH471083 Genomic DNA. Translation: EAW54142.1. BC028239 mRNA. Translation: AAH28239.1. AB014589 mRNA. Translation: BAA31664.1.
IPI	IPI00550906.
RefSeq	NP_056050.1. NM_015235.2.
UniGene	Hs.591358.
3D structure databases
ProteinModelPortal	Q9H0L4.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9H0L4. 3 interactions.
MINT	MINT-1461400.
STRING	9606.ENSP00000332444.
PTM databases
PhosphoSite	Q9H0L4.
Polymorphism databases
DMDM	71153234.
2D gel databases
REPRODUCTION-2DPAGE	IPI00550906.
Proteomic databases
PaxDb	Q9H0L4.
PeptideAtlas	Q9H0L4.
PRIDE	Q9H0L4.
Protocols and materials databases
DNASU	23283.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000331173; ENSP00000332444; ENSG00000177613.
GeneID	23283.
KEGG	hsa:23283.
UCSC	uc001jjp.3. human.
Organism-specific databases
CTD	23283.
GeneCards	GC10M053455.
HGNC	HGNC:17086. CSTF2T.
HPA	HPA039216.
MIM	611968. gene.
neXtProt	NX_Q9H0L4.
PharmGKB	PA134933809.
HUGE	Search...
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG0724.
HOGENOM	HOG000214373.
HOVERGEN	HBG051145.
InParanoid	Q9H0L4.
KO	K14407.
OMA	METCAME.
OrthoDB	EOG4640BW.
PhylomeDB	Q9H0L4.
Gene expression databases
Bgee	Q9H0L4.
CleanEx	HS_CSTF2T.
Genevestigator	Q9H0L4.
GermOnline	ENSG00000177613. Homo sapiens.
Family and domain databases
Gene3D	3.30.70.330. 1 hit.
InterPro	IPR025742. CSTF2_hinge. IPR026896. CSTF_C. IPR012677. Nucleotide-bd_a/b_plait. IPR000504. RRM_dom. [Graphical view]
Pfam	PF14327. CSTF2_hinge. 1 hit. PF14304. CSTF_C. 1 hit. PF00076. RRM_1. 1 hit. [Graphical view]
SMART	SM00360. RRM. 1 hit. [Graphical view]
PROSITE	PS50102. RRM. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	23283.
NextBio	45080.
SOURCE	Search...

Entry information

Entry name

CSTFT_HUMAN

Accession

Primary (citable) accession number: Q9H0L4
Secondary accession number(s): B2RAR9 , O75174, Q53HK6, Q7LGE8, Q8N6T1

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2005
Last sequence update:	March 1, 2001
Last modified:	May 1, 2013
This is version 98 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.