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Protein

Poly [ADP-ribose] polymerase 12

Gene

PARP12

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

NAD+ + (ADP-D-ribosyl)(n)-acceptor = nicotinamide + (ADP-D-ribosyl)(n+1)-acceptor.PROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri94 – 11926C3H1-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri150 – 17930C3H1-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri180 – 20223C3H1-type 3PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri270 – 29728C3H1-type 4PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri271 – 29626C3H1-type 3PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. NAD+ ADP-ribosyltransferase activity Source: UniProtKB-EC
  3. poly(A) RNA binding Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Ligandi

Metal-binding, NAD, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Poly [ADP-ribose] polymerase 12 (EC:2.4.2.30)
Short name:
PARP-12
Alternative name(s):
ADP-ribosyltransferase diphtheria toxin-like 12
Short name:
ARTD12
Zinc finger CCCH domain-containing protein 1
Gene namesi
Name:PARP12
Synonyms:ZC3HDC1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 7

Organism-specific databases

HGNCiHGNC:21919. PARP12.

Subcellular locationi

Nucleus Curated

GO - Cellular componenti

  1. nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134953063.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 701701Poly [ADP-ribose] polymerase 12PRO_0000211341Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei258 – 2581Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ9H0J9.
PaxDbiQ9H0J9.
PRIDEiQ9H0J9.

PTM databases

PhosphoSiteiQ9H0J9.

Expressioni

Gene expression databases

BgeeiQ9H0J9.
CleanExiHS_PARP12.
ExpressionAtlasiQ9H0J9. baseline and differential.
GenevestigatoriQ9H0J9.

Organism-specific databases

HPAiHPA003584.

Interactioni

Protein-protein interaction databases

BioGridi122274. 12 interactions.
IntActiQ9H0J9. 4 interactions.
STRINGi9606.ENSP00000263549.

Structurei

Secondary structure

1
701
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi499 – 5024Combined sources
Helixi508 – 51811Combined sources
Beta strandi524 – 53310Combined sources
Helixi535 – 55016Combined sources
Turni551 – 5544Combined sources
Beta strandi559 – 5657Combined sources
Helixi568 – 5703Combined sources
Helixi571 – 5777Combined sources
Turni581 – 5844Combined sources
Beta strandi593 – 6008Combined sources
Helixi601 – 6055Combined sources
Beta strandi611 – 62313Combined sources
Beta strandi626 – 6294Combined sources
Beta strandi649 – 6535Combined sources
Beta strandi655 – 6573Combined sources
Beta strandi660 – 6645Combined sources
Helixi666 – 6683Combined sources
Beta strandi669 – 67911Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2PQFX-ray2.20A/B/C/D/E/F489-684[»]
ProteinModelPortaliQ9H0J9.
SMRiQ9H0J9. Positions 496-680.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9H0J9.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini298 – 36164WWE 1PROSITE-ProRule annotationAdd
BLAST
Domaini364 – 45895WWE 2PROSITE-ProRule annotationAdd
BLAST
Domaini484 – 698215PARP catalyticPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi58 – 614Poly-Ala

Sequence similaritiesi

Contains 4 C3H1-type zinc fingers.PROSITE-ProRule annotation
Contains 1 PARP catalytic domain.PROSITE-ProRule annotation
Contains 2 WWE domains.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri94 – 11926C3H1-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri150 – 17930C3H1-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri180 – 20223C3H1-type 3PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri270 – 29728C3H1-type 4PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri271 – 29626C3H1-type 3PROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiNOG83866.
GeneTreeiENSGT00760000119084.
HOGENOMiHOG000236279.
HOVERGENiHBG050384.
InParanoidiQ9H0J9.
KOiK15259.
OMAiRWQFLDR.
OrthoDBiEOG7P5T0J.
PhylomeDBiQ9H0J9.
TreeFamiTF338389.

Family and domain databases

Gene3Di3.90.228.10. 1 hit.
InterProiIPR012317. Poly(ADP-ribose)pol_cat_dom.
IPR004170. WWE-dom.
IPR000571. Znf_CCCH.
[Graphical view]
PfamiPF00644. PARP. 1 hit.
PF00642. zf-CCCH. 1 hit.
[Graphical view]
SMARTiSM00356. ZnF_C3H1. 3 hits.
[Graphical view]
PROSITEiPS51059. PARP_CATALYTIC. 1 hit.
PS50918. WWE. 2 hits.
PS50103. ZF_C3H1. 4 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9H0J9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAQAGVVGEV TQVLCAAGGA LELPELRRRL RMGLSADALE RLLRQRGRFV
60 70 80 90 100
VAVRAGGAAA APERVVLAAS PLRLCRAHQG SKPGCVGLCA QLHLCRFMVY
110 120 130 140 150
GACKFLRAGK NCRNSHSLTT EHNLSVLRTH GVDHLSYNEL CQLLFQNDPW
160 170 180 190 200
LLPEICQHYN KGDGPHGSCA FQKQCIKLHI CQYFLQGECK FGTSCKRSHD
210 220 230 240 250
FSNSENLEKL EKLGMSSDLV SRLPTIYRNA HDIKNKSSAP SRVPPLFVPQ
260 270 280 290 300
GTSERKDSSG SVSPNTLSQE EGDQICLYHI RKSCSFQDKC HRVHFHLPYR
310 320 330 340 350
WQFLDRGKWE DLDNMELIEE AYCNPKIERI LCSESASTFH SHCLNFNAMT
360 370 380 390 400
YGATQARRLS TASSVTKPPH FILTTDWIWY WSDEFGSWQE YGRQGTVHPV
410 420 430 440 450
TTVSSSDVEK AYLAYCTPGS DGQAATLKFQ AGKHNYELDF KAFVQKNLVY
460 470 480 490 500
GTTKKVCRRP KYVSPQDVTT MQTCNTKFPG PKSIPDYWDS SALPDPGFQK
510 520 530 540 550
ITLSSSSEEY QKVWNLFNRT LPFYFVQKIE RVQNLALWEV YQWQKGQMQK
560 570 580 590 600
QNGGKAVDER QLFHGTSAIF VDAICQQNFD WRVCGVHGTS YGKGSYFARD
610 620 630 640 650
AAYSHHYSKS DTQTHTMFLA RVLVGEFVRG NASFVRPPAK EGWSNAFYDS
660 670 680 690 700
CVNSVSDPSI FVIFEKHQVY PEYVIQYTTS SKPSVTPSIL LALGSLFSSR

Q
Length:701
Mass (Da):79,064
Last modified:February 28, 2001 - v1
Checksum:i35152182F24CD215
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti444 – 4507VQKNLVY → MGGFGQH (PubMed:14702039).Curated
Sequence conflicti462 – 4621Y → C in BAB15457 (PubMed:14702039).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti293 – 2931V → I.
Corresponds to variant rs34111764 [ dbSNP | Ensembl ].
VAR_050463
Natural varianti463 – 4631V → M.
Corresponds to variant rs35456446 [ dbSNP | Ensembl ].
VAR_050464
Natural varianti620 – 6201A → V.
Corresponds to variant rs17161356 [ dbSNP | Ensembl ].
VAR_050465

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL136766 mRNA. Translation: CAB66700.1.
AL137255 mRNA. Translation: CAB70657.1.
AC004849 Genomic DNA. Translation: AAS00360.1.
AC025816 Genomic DNA. Translation: AAF66161.1.
AC004961 Genomic DNA. No translation available.
BC081541 mRNA. Translation: AAH81541.1.
AK026346 mRNA. Translation: BAB15457.1.
CCDSiCCDS5857.1.
PIRiT46327.
RefSeqiNP_073587.1. NM_022750.3.
UniGeneiHs.12646.
Hs.308710.

Genome annotation databases

EnsembliENST00000263549; ENSP00000263549; ENSG00000059378.
GeneIDi64761.
KEGGihsa:64761.
UCSCiuc003vvl.1. human.

Polymorphism databases

DMDMi47117630.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL136766 mRNA. Translation: CAB66700.1.
AL137255 mRNA. Translation: CAB70657.1.
AC004849 Genomic DNA. Translation: AAS00360.1.
AC025816 Genomic DNA. Translation: AAF66161.1.
AC004961 Genomic DNA. No translation available.
BC081541 mRNA. Translation: AAH81541.1.
AK026346 mRNA. Translation: BAB15457.1.
CCDSiCCDS5857.1.
PIRiT46327.
RefSeqiNP_073587.1. NM_022750.3.
UniGeneiHs.12646.
Hs.308710.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2PQFX-ray2.20A/B/C/D/E/F489-684[»]
ProteinModelPortaliQ9H0J9.
SMRiQ9H0J9. Positions 496-680.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi122274. 12 interactions.
IntActiQ9H0J9. 4 interactions.
STRINGi9606.ENSP00000263549.

Chemistry

ChEMBLiCHEMBL2429709.

PTM databases

PhosphoSiteiQ9H0J9.

Polymorphism databases

DMDMi47117630.

Proteomic databases

MaxQBiQ9H0J9.
PaxDbiQ9H0J9.
PRIDEiQ9H0J9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000263549; ENSP00000263549; ENSG00000059378.
GeneIDi64761.
KEGGihsa:64761.
UCSCiuc003vvl.1. human.

Organism-specific databases

CTDi64761.
GeneCardsiGC07M139723.
HGNCiHGNC:21919. PARP12.
HPAiHPA003584.
MIMi612481. gene.
neXtProtiNX_Q9H0J9.
PharmGKBiPA134953063.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG83866.
GeneTreeiENSGT00760000119084.
HOGENOMiHOG000236279.
HOVERGENiHBG050384.
InParanoidiQ9H0J9.
KOiK15259.
OMAiRWQFLDR.
OrthoDBiEOG7P5T0J.
PhylomeDBiQ9H0J9.
TreeFamiTF338389.

Miscellaneous databases

ChiTaRSiPARP12. human.
EvolutionaryTraceiQ9H0J9.
GeneWikiiPARP12.
GenomeRNAii64761.
NextBioi66743.
PROiQ9H0J9.
SOURCEiSearch...

Gene expression databases

BgeeiQ9H0J9.
CleanExiHS_PARP12.
ExpressionAtlasiQ9H0J9. baseline and differential.
GenevestigatoriQ9H0J9.

Family and domain databases

Gene3Di3.90.228.10. 1 hit.
InterProiIPR012317. Poly(ADP-ribose)pol_cat_dom.
IPR004170. WWE-dom.
IPR000571. Znf_CCCH.
[Graphical view]
PfamiPF00644. PARP. 1 hit.
PF00642. zf-CCCH. 1 hit.
[Graphical view]
SMARTiSM00356. ZnF_C3H1. 3 hits.
[Graphical view]
PROSITEiPS51059. PARP_CATALYTIC. 1 hit.
PS50918. WWE. 2 hits.
PS50103. ZF_C3H1. 4 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Testis.
  2. "The DNA sequence of human chromosome 7."
    Hillier L.W., Fulton R.S., Fulton L.A., Graves T.A., Pepin K.H., Wagner-McPherson C., Layman D., Maas J., Jaeger S., Walker R., Wylie K., Sekhon M., Becker M.C., O'Laughlin M.D., Schaller M.E., Fewell G.A., Delehaunty K.D., Miner T.L.
    , Nash W.E., Cordes M., Du H., Sun H., Edwards J., Bradshaw-Cordum H., Ali J., Andrews S., Isak A., Vanbrunt A., Nguyen C., Du F., Lamar B., Courtney L., Kalicki J., Ozersky P., Bielicki L., Scott K., Holmes A., Harkins R., Harris A., Strong C.M., Hou S., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Leonard S., Rohlfing T., Rock S.M., Tin-Wollam A.-M., Abbott A., Minx P., Maupin R., Strowmatt C., Latreille P., Miller N., Johnson D., Murray J., Woessner J.P., Wendl M.C., Yang S.-P., Schultz B.R., Wallis J.W., Spieth J., Bieri T.A., Nelson J.O., Berkowicz N., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Bedell J.A., Mardis E.R., Clifton S.W., Chissoe S.L., Marra M.A., Raymond C., Haugen E., Gillett W., Zhou Y., James R., Phelps K., Iadanoto S., Bubb K., Simms E., Levy R., Clendenning J., Kaul R., Kent W.J., Furey T.S., Baertsch R.A., Brent M.R., Keibler E., Flicek P., Bork P., Suyama M., Bailey J.A., Portnoy M.E., Torrents D., Chinwalla A.T., Gish W.R., Eddy S.R., McPherson J.D., Olson M.V., Eichler E.E., Green E.D., Waterston R.H., Wilson R.K.
    Nature 424:157-164(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Testis.
  4. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 444-701.
    Tissue: Small intestine.
  5. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  6. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
    Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
    Sci. Signal. 3:RA3-RA3(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  7. "Toward a unified nomenclature for mammalian ADP-ribosyltransferases."
    Hottiger M.O., Hassa P.O., Luscher B., Schuler H., Koch-Nolte F.
    Trends Biochem. Sci. 35:208-219(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NOMENCLATURE.
  8. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."
    Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L., Ye M., Zou H.
    J. Proteomics 96:253-262(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-258, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiPAR12_HUMAN
AccessioniPrimary (citable) accession number: Q9H0J9
Secondary accession number(s): Q9H610, Q9NP36, Q9NTI3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 9, 2004
Last sequence update: February 28, 2001
Last modified: March 3, 2015
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 7
    Human chromosome 7: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.