Reviewed, UniProtKB/Swiss-Prot Q9H0J9 (PAR12_HUMAN)

Names and origin

Protein names

Recommended name:
    Poly [ADP-ribose] polymerase 12
      Short name=PARP-12
    EC=2.4.2.30
Alternative name(s):
    Zinc finger CCCH domain-containing protein 1

Gene names

Name:	PARP12
Synonyms:	ZC3HDC1

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	701 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	NAD+ + (ADP-D-ribosyl)(n)-acceptor = nicotinamide + (ADP-D-ribosyl)(n+1)-acceptor.
Subcellular location	Nucleus Probable.
Sequence similarities	Contains 4 C3H1-type zinc fingers. Contains 1 PARP catalytic domain. Contains 2 WWE domains.

Ontologies

Keywords
Cellular component	Nucleus
Coding sequence diversity	Polymorphism
Domain	Repeat Zinc-finger
Ligand	Metal-binding NAD Zinc
Molecular function	Transferase
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NAD+ ADP-ribosyltransferase activity Inferred from electronic annotation. Source: EC nucleic acid binding Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 701

701

Poly [ADP-ribose] polymerase 12

PRO_0000211341

Regions

Domain

298 – 361

64

WWE 1

Domain

364 – 458

95

WWE 2

Domain

484 – 698

215

PARP catalytic

Zinc finger

94 – 119

26

C3H1-type 1

Zinc finger

150 – 179

30

C3H1-type 2

Zinc finger

180 – 202

23

C3H1-type 3

Zinc finger

270 – 297

28

C3H1-type 4

Zinc finger

271 – 296

26

C3H1-type 3

Compositional bias

58 – 61

4

Poly-Ala

Amino acid modifications

Modified residue

258

1

Phosphoserine Ref.5

Modified residue

263

1

Phosphoserine Ref.5

Natural variations

Natural variant

293

1

V → I: dbSNP rs34111764.

VAR_050463

Natural variant

463

1

V → M: dbSNP rs35456446.

VAR_050464

Natural variant

620

1

A → V: dbSNP rs17161356.

VAR_050465

Experimental info

Sequence conflict

444 – 450

7

VQKNLVY → MGGFGQH Ref.4

Sequence conflict

462

1

Y → C in BAB15457. Ref.4

Secondary structure

1

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701

Helix Strand Turn

Details...

Beta strand

499 – 502

4

Helix

508 – 518

11

Beta strand

524 – 533

10

Helix

535 – 550

16

Turn

551 – 554

4

Beta strand

559 – 565

7

Helix

568 – 570

3

Helix

571 – 577

7

Turn

581 – 584

4

Beta strand

593 – 600

8

Helix

601 – 605

5

Beta strand

611 – 623

13

Beta strand

626 – 629

4

Beta strand

649 – 653

5

Beta strand

655 – 657

3

Beta strand

660 – 664

5

Helix

666 – 668

3

Beta strand

669 – 679

11

Sequences

	Sequence		Length	Mass (Da)
	Q9H0J9-1 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: 35152182F24CD215	FASTA	701	79,064
	10 20 30 40 50 60 MAQAGVVGEV TQVLCAAGGA LELPELRRRL RMGLSADALE RLLRQRGRFV VAVRAGGAAA 70 80 90 100 110 120 APERVVLAAS PLRLCRAHQG SKPGCVGLCA QLHLCRFMVY GACKFLRAGK NCRNSHSLTT 130 140 150 160 170 180 EHNLSVLRTH GVDHLSYNEL CQLLFQNDPW LLPEICQHYN KGDGPHGSCA FQKQCIKLHI 190 200 210 220 230 240 CQYFLQGECK FGTSCKRSHD FSNSENLEKL EKLGMSSDLV SRLPTIYRNA HDIKNKSSAP 250 260 270 280 290 300 SRVPPLFVPQ GTSERKDSSG SVSPNTLSQE EGDQICLYHI RKSCSFQDKC HRVHFHLPYR 310 320 330 340 350 360 WQFLDRGKWE DLDNMELIEE AYCNPKIERI LCSESASTFH SHCLNFNAMT YGATQARRLS 370 380 390 400 410 420 TASSVTKPPH FILTTDWIWY WSDEFGSWQE YGRQGTVHPV TTVSSSDVEK AYLAYCTPGS 430 440 450 460 470 480 DGQAATLKFQ AGKHNYELDF KAFVQKNLVY GTTKKVCRRP KYVSPQDVTT MQTCNTKFPG 490 500 510 520 530 540 PKSIPDYWDS SALPDPGFQK ITLSSSSEEY QKVWNLFNRT LPFYFVQKIE RVQNLALWEV 550 560 570 580 590 600 YQWQKGQMQK QNGGKAVDER QLFHGTSAIF VDAICQQNFD WRVCGVHGTS YGKGSYFARD 610 620 630 640 650 660 AAYSHHYSKS DTQTHTMFLA RVLVGEFVRG NASFVRPPAK EGWSNAFYDS CVNSVSDPSI 670 680 690 700 FVIFEKHQVY PEYVIQYTTS SKPSVTPSIL LALGSLFSSR Q

References

[1]	"Towards a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs." Wiemann S., Weil B., Wellenreuther R., Gassenhuber J., Glassl S., Ansorge W., Boecher M., Bloecker H., Bauersachs S., Blum H., Lauber J., Duesterhoeft A., Beyer A., Koehrer K., Strack N., Mewes H.-W., Ottenwaelder B., Obermaier B. , Tampe J., Heubner D., Wambutt R., Korn B., Klein M., Poustka A. Genome Res. 11:422-435(2001) [PubMed: 11230166] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Testis.
[2]	"The DNA sequence of human chromosome 7." Hillier L.W., Fulton R.S., Fulton L.A., Graves T.A., Pepin K.H., Wagner-McPherson C., Layman D., Maas J., Jaeger S., Walker R., Wylie K., Sekhon M., Becker M.C., O'Laughlin M.D., Schaller M.E., Fewell G.A., Delehaunty K.D., Miner T.L. , Nash W.E., Cordes M., Du H., Sun H., Edwards J., Bradshaw-Cordum H., Ali J., Andrews S., Isak A., Vanbrunt A., Nguyen C., Du F., Lamar B., Courtney L., Kalicki J., Ozersky P., Bielicki L., Scott K., Holmes A., Harkins R., Harris A., Strong C.M., Hou S., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Leonard S., Rohlfing T., Rock S.M., Tin-Wollam A.-M., Abbott A., Minx P., Maupin R., Strowmatt C., Latreille P., Miller N., Johnson D., Murray J., Woessner J.P., Wendl M.C., Yang S.-P., Schultz B.R., Wallis J.W., Spieth J., Bieri T.A., Nelson J.O., Berkowicz N., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Bedell J.A., Mardis E.R., Clifton S.W., Chissoe S.L., Marra M.A., Raymond C., Haugen E., Gillett W., Zhou Y., James R., Phelps K., Iadanoto S., Bubb K., Simms E., Levy R., Clendenning J., Kaul R., Kent W.J., Furey T.S., Baertsch R.A., Brent M.R., Keibler E., Flicek P., Bork P., Suyama M., Bailey J.A., Portnoy M.E., Torrents D., Chinwalla A.T., Gish W.R., Eddy S.R., McPherson J.D., Olson M.V., Eichler E.E., Green E.D., Waterston R.H., Wilson R.K. Nature 424:157-164(2003) [PubMed: 12853948] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Testis.
[4]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 444-701. Tissue: Small intestine.
[5]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-258 AND SER-263, MASS SPECTROMETRY.

Cross-references

Sequence databases

EMBLGenBankDDBJ

AL136766 mRNA. Translation: CAB66700.1.
AL137255 mRNA. Translation: CAB70657.1.
AC004849 Genomic DNA. Translation: AAS00360.1.
AC025816 Genomic DNA. Translation: AAF66161.1.
AC004961 Genomic DNA. No translation available.
BC081541 mRNA. Translation: AAH81541.1.
AK026346 mRNA. Translation: BAB15457.1.

IPI

IPI00009355.

PIR

T46327.

RefSeq

NP_073587.1.

UniGene

Hs.12646
Hs.308710

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2PQF	X-ray	2.20	A/B/C/D/E/F	489-684	[»]

ModBase

Search...

Protein-protein interaction databases

STRING

Q9H0J9.

PTM databases

PhosphoSite

Q9H0J9.

Proteomic databases

PRIDE

Q9H0J9.

Genome annotation databases

Ensembl

ENST00000263549; ENSP00000263549; ENSG00000059378; Homo sapiens. [Genome view]

GeneID

64761.

KEGG

hsa:64761.

UCSC

uc003vvl.1. human.

Organism-specific databases

CTD

64761.

GeneCards

GC07M139370.

HGNC

HGNC:21919. PARP12.

HPA

HPA003584.

MIM

612481. gene.

PharmGKB

PA134953063.

GenAtlas

Search...

Phylogenomic databases

HOVERGEN

Q9H0J9.

OMA

CSFQKQC.

Enzyme and pathway databases

BRENDA

2.4.2.30. 247.

Gene expression databases

ArrayExpress

Q9H0J9.

Bgee

Q9H0J9.

CleanEx

HS_PARP12.

Genevestigator

Q9H0J9.

GermOnline

ENSG00000059378. Homo sapiens.

Family and domain databases

InterPro

IPR012317. PARP_catalytic.
IPR004170. WWE-dom.
IPR000571. Znf_CCCH.
[Graphical view]

Pfam

PF00644. PARP. 1 hit.
PF00642. zf-CCCH. 3 hits.
[Graphical view]

SMART

SM00356. ZnF_C3H1. 3 hits.
[Graphical view]

PROSITE

PS51059. PARP_CATALYTIC. 1 hit.
PS50918. WWE. 2 hits.
PS50103. ZF_C3H1. 4 hits.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

66743.

SOURCE

Search...

Entry information

Entry name

PAR12_HUMAN

Accession

Primary (citable) accession number: Q9H0J9
Secondary accession number(s): Q9H610, Q9NP36, Q9NTI3

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2004
Last sequence update:	March 1, 2001
Last modified:	November 3, 2009
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.